In vitro stimulation of Balb/c and C57 BL/6 splenocytes by a recombinantly produced banana lectin isoform results in both a proliferation of T cells and an increased secretion of interferon-gamma
Само за регистроване кориснике
2010
Аутори
Stojanović, MarijanaŽivković, Irena
Petrušić, Vladimir
Kosec, Duško
Dimitrijević, Rajna
Jankov, Ratko
Dimitrijević, Ljiljana
Gavrović-Jankulović, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Lectins are widely used in many types of assay but some lectins such as banana lectin (BanLec) are recognised as potent immunostimulators Although BanLec's structure and binding characteristics are now familiar, its immunostimulatory potential has not yet been fully explored The synthesis by recombinant technology of a BanLec isoform (rBanLec) whose binding properties are similar to its natural counterpart has made it possible to overcome the twin problems of natural BanLec's microheterogeneity and low availability This study's aim is to explore the immunostimulatory potential of rBanLec in the murine model Analyses of the responses of Balb/c- and C57 BL/6-originated splenocytes to in vitro rBanLec stimulation were performed to examine the dependency of rBanLec's immunostimulatory potential upon the splenocytes' genetic background It is shown that the responses of Balb/c- and C57 BL/6-originated splenocytes to rBanLec stimulation differ both qualitatively and in intensity. The hallmark...s of the induced responses are T lymphocyte proliferation and intensive interferon-gamma secretion Both phenomena are more marked in Balb/c-originated cultures; Balb/c-originated lymphocytes produce interleukin (IL)-4 and IL-10 following rBanLec stimulation Out results demonstrate that any responses to rBanLec stimulation are highly dependent upon genetic background. they suggest that genetic background must be an important consideration in any further investigations using animal models or when exploring rBanLec's potential human applications (C) 2009 Elsevier B V. All rights reserved
Кључне речи:
Interferon-gamma / Murine lymphocytes / Proliferation / Recombinant banana lectinИзвор:
International Immunopharmacology, 2010, 10, 1, 120-129Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Испитивање структуре и функције биолошки важних макромолекула у физиолошким и патолошким стањима (RS-MESTD-MPN2006-2010-142020)
DOI: 10.1016/j.intimp.2009.10.007
ISSN: 1567-5769
PubMed: 19874914
WoS: 000274361700017
Scopus: 2-s2.0-73449101067
Институција/група
TorlakTY - JOUR AU - Stojanović, Marijana AU - Živković, Irena AU - Petrušić, Vladimir AU - Kosec, Duško AU - Dimitrijević, Rajna AU - Jankov, Ratko AU - Dimitrijević, Ljiljana AU - Gavrović-Jankulović, Marija PY - 2010 UR - http://intor.torlakinstitut.com/handle/123456789/300 AB - Lectins are widely used in many types of assay but some lectins such as banana lectin (BanLec) are recognised as potent immunostimulators Although BanLec's structure and binding characteristics are now familiar, its immunostimulatory potential has not yet been fully explored The synthesis by recombinant technology of a BanLec isoform (rBanLec) whose binding properties are similar to its natural counterpart has made it possible to overcome the twin problems of natural BanLec's microheterogeneity and low availability This study's aim is to explore the immunostimulatory potential of rBanLec in the murine model Analyses of the responses of Balb/c- and C57 BL/6-originated splenocytes to in vitro rBanLec stimulation were performed to examine the dependency of rBanLec's immunostimulatory potential upon the splenocytes' genetic background It is shown that the responses of Balb/c- and C57 BL/6-originated splenocytes to rBanLec stimulation differ both qualitatively and in intensity. The hallmarks of the induced responses are T lymphocyte proliferation and intensive interferon-gamma secretion Both phenomena are more marked in Balb/c-originated cultures; Balb/c-originated lymphocytes produce interleukin (IL)-4 and IL-10 following rBanLec stimulation Out results demonstrate that any responses to rBanLec stimulation are highly dependent upon genetic background. they suggest that genetic background must be an important consideration in any further investigations using animal models or when exploring rBanLec's potential human applications (C) 2009 Elsevier B V. All rights reserved PB - Elsevier, Amsterdam T2 - International Immunopharmacology T1 - In vitro stimulation of Balb/c and C57 BL/6 splenocytes by a recombinantly produced banana lectin isoform results in both a proliferation of T cells and an increased secretion of interferon-gamma EP - 129 IS - 1 SP - 120 VL - 10 DO - 10.1016/j.intimp.2009.10.007 ER -
@article{ author = "Stojanović, Marijana and Živković, Irena and Petrušić, Vladimir and Kosec, Duško and Dimitrijević, Rajna and Jankov, Ratko and Dimitrijević, Ljiljana and Gavrović-Jankulović, Marija", year = "2010", abstract = "Lectins are widely used in many types of assay but some lectins such as banana lectin (BanLec) are recognised as potent immunostimulators Although BanLec's structure and binding characteristics are now familiar, its immunostimulatory potential has not yet been fully explored The synthesis by recombinant technology of a BanLec isoform (rBanLec) whose binding properties are similar to its natural counterpart has made it possible to overcome the twin problems of natural BanLec's microheterogeneity and low availability This study's aim is to explore the immunostimulatory potential of rBanLec in the murine model Analyses of the responses of Balb/c- and C57 BL/6-originated splenocytes to in vitro rBanLec stimulation were performed to examine the dependency of rBanLec's immunostimulatory potential upon the splenocytes' genetic background It is shown that the responses of Balb/c- and C57 BL/6-originated splenocytes to rBanLec stimulation differ both qualitatively and in intensity. The hallmarks of the induced responses are T lymphocyte proliferation and intensive interferon-gamma secretion Both phenomena are more marked in Balb/c-originated cultures; Balb/c-originated lymphocytes produce interleukin (IL)-4 and IL-10 following rBanLec stimulation Out results demonstrate that any responses to rBanLec stimulation are highly dependent upon genetic background. they suggest that genetic background must be an important consideration in any further investigations using animal models or when exploring rBanLec's potential human applications (C) 2009 Elsevier B V. All rights reserved", publisher = "Elsevier, Amsterdam", journal = "International Immunopharmacology", title = "In vitro stimulation of Balb/c and C57 BL/6 splenocytes by a recombinantly produced banana lectin isoform results in both a proliferation of T cells and an increased secretion of interferon-gamma", pages = "129-120", number = "1", volume = "10", doi = "10.1016/j.intimp.2009.10.007" }
Stojanović, M., Živković, I., Petrušić, V., Kosec, D., Dimitrijević, R., Jankov, R., Dimitrijević, L.,& Gavrović-Jankulović, M.. (2010). In vitro stimulation of Balb/c and C57 BL/6 splenocytes by a recombinantly produced banana lectin isoform results in both a proliferation of T cells and an increased secretion of interferon-gamma. in International Immunopharmacology Elsevier, Amsterdam., 10(1), 120-129. https://doi.org/10.1016/j.intimp.2009.10.007
Stojanović M, Živković I, Petrušić V, Kosec D, Dimitrijević R, Jankov R, Dimitrijević L, Gavrović-Jankulović M. In vitro stimulation of Balb/c and C57 BL/6 splenocytes by a recombinantly produced banana lectin isoform results in both a proliferation of T cells and an increased secretion of interferon-gamma. in International Immunopharmacology. 2010;10(1):120-129. doi:10.1016/j.intimp.2009.10.007 .
Stojanović, Marijana, Živković, Irena, Petrušić, Vladimir, Kosec, Duško, Dimitrijević, Rajna, Jankov, Ratko, Dimitrijević, Ljiljana, Gavrović-Jankulović, Marija, "In vitro stimulation of Balb/c and C57 BL/6 splenocytes by a recombinantly produced banana lectin isoform results in both a proliferation of T cells and an increased secretion of interferon-gamma" in International Immunopharmacology, 10, no. 1 (2010):120-129, https://doi.org/10.1016/j.intimp.2009.10.007 . .