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dc.creatorStanojević, Stanislava
dc.creatorVujić, Vesna
dc.creatorMitić, Katarina
dc.creatorKuštrimović, Nataša
dc.creatorKovačević-Jovanović, Vesna
dc.creatorMiletić, Tatjana
dc.creatorDimitrijević, Mirjana
dc.date.accessioned2021-02-18T10:32:38Z
dc.date.available2021-02-18T10:32:38Z
dc.date.issued2008
dc.identifier.issn0143-4179
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/259
dc.description.abstractWe investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.en
dc.publisherChurchill Livingstone, Edinburgh
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/145049/RS//
dc.rightsrestrictedAccess
dc.sourceNeuropeptides
dc.subjectbeta-funaltrexamine (beta FNA)en
dc.subjectbenzylidenenaltrexone (BNTX)en
dc.subjecthydrogen peroxide (H2O2) releaseen
dc.subjectmethionine-enkephalin (MET)en
dc.subjectnaltriben (NTB)en
dc.subjectnaltrindole (NTI)en
dc.subjectnor-binaltorphimine (norBNI)en
dc.subjectmu, delta(1), delta(2), kappa opioid receptorsen
dc.subjectrat peritoneal macrophagesen
dc.titleMethionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptorsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage158
dc.citation.issue2
dc.citation.other42(2): 147-158
dc.citation.rankM22
dc.citation.spage147
dc.citation.volume42
dc.identifier.doi10.1016/j.npep.2007.12.004
dc.identifier.pmid18237778
dc.identifier.rcubconv_206
dc.identifier.scopus2-s2.0-39949085768
dc.identifier.wos000254779400003
dc.type.versionpublishedVersion


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