Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors
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2008
Authors
Stanojević, StanislavaVujić, Vesna
Mitić, Katarina
Kuštrimović, Nataša

Kovačević-Jovanović, Vesna
Miletić, Tatjana
Dimitrijević, Mirjana

Article (Published version)

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We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specifi...c for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.
Keywords:
beta-funaltrexamine (beta FNA) / benzylidenenaltrexone (BNTX) / hydrogen peroxide (H2O2) release / methionine-enkephalin (MET) / naltriben (NTB) / naltrindole (NTI) / nor-binaltorphimine (norBNI) / mu, delta(1), delta(2), kappa opioid receptors / rat peritoneal macrophagesSource:
Neuropeptides, 2008, 42, 2, 147-158Publisher:
- Churchill Livingstone, Edinburgh
Funding / projects:
DOI: 10.1016/j.npep.2007.12.004
ISSN: 0143-4179
PubMed: 18237778
WoS: 000254779400003
Scopus: 2-s2.0-39949085768
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TorlakTY - JOUR AU - Stanojević, Stanislava AU - Vujić, Vesna AU - Mitić, Katarina AU - Kuštrimović, Nataša AU - Kovačević-Jovanović, Vesna AU - Miletić, Tatjana AU - Dimitrijević, Mirjana PY - 2008 UR - http://intor.torlakinstitut.com/handle/123456789/259 AB - We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved. PB - Churchill Livingstone, Edinburgh T2 - Neuropeptides T1 - Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors EP - 158 IS - 2 SP - 147 VL - 42 DO - 10.1016/j.npep.2007.12.004 ER -
@article{ author = "Stanojević, Stanislava and Vujić, Vesna and Mitić, Katarina and Kuštrimović, Nataša and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Dimitrijević, Mirjana", year = "2008", abstract = "We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.", publisher = "Churchill Livingstone, Edinburgh", journal = "Neuropeptides", title = "Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors", pages = "158-147", number = "2", volume = "42", doi = "10.1016/j.npep.2007.12.004" }
Stanojević, S., Vujić, V., Mitić, K., Kuštrimović, N., Kovačević-Jovanović, V., Miletić, T.,& Dimitrijević, M.. (2008). Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors. in Neuropeptides Churchill Livingstone, Edinburgh., 42(2), 147-158. https://doi.org/10.1016/j.npep.2007.12.004
Stanojević S, Vujić V, Mitić K, Kuštrimović N, Kovačević-Jovanović V, Miletić T, Dimitrijević M. Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors. in Neuropeptides. 2008;42(2):147-158. doi:10.1016/j.npep.2007.12.004 .
Stanojević, Stanislava, Vujić, Vesna, Mitić, Katarina, Kuštrimović, Nataša, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Dimitrijević, Mirjana, "Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors" in Neuropeptides, 42, no. 2 (2008):147-158, https://doi.org/10.1016/j.npep.2007.12.004 . .