The aim of our current study was to investigate the effect of acute exposure to electric tail shock stress (ES) and to a stress witnessing procedure (SW), as models for physical and psychological stress paradigms, respectively, on phagocytosis and H2O2 production in peritoneal macrophages isolated from Albino Oxford (AO) and Dark Agouti (DA) rats. In addition, we studied the in vitro effects of methionine-enkephalin (ME) on phagocytosis and H2O2 production in peritoneal macrophages isolated from both AO and DA rats that had been exposed to ES and SW procedures. The results showed that peritoneal macrophages isolated from DA rats were less sensitive to the suppressive effects of ES and SW than macrophages isolated from AO rats. In vitro treatment of macrophages isolated from AO rats with ME mimicked to some extent the suppressive effects of ES and SW on phagocytosis and H2O2 production and additionally diminished H2O2 release in macrophages isolated from AO rats previously exposed to ES... or SW ME did not have any effect on phagocytosis in macrophages isolated from DA rats, but changed H2O2 production in a concentration-dependent manner. In macrophages isolated from DA rats previously exposed to stress the effect of ME was dependent on the macrophage function tested and the particular stress paradigm employed. Our results emphasise the fact that both beneficial and detrimental effects of stress on immune system functions could be attributed to the individual variations in the macrophage's response to stress mediators. (c) 2006 Elsevier Inc. All rights reserved.
Keywords:
Albino Oxford rats / Dark Agouti rats / electric tail shock stress / stress witnessing procedure / methionine-enkephalin / peritoneal macrophages / phagocytosis / hydrogen peroxide production
TY - JOUR
AU - Stanojević, Stanislava
AU - Mitić, Katarina
AU - Vujić, Vesna
AU - Kovačević-Jovanović, Vesna
AU - Dimitrijević, Mirjana
PY - 2007
UR - http://intor.torlakinstitut.com/handle/123456789/228
AB - The aim of our current study was to investigate the effect of acute exposure to electric tail shock stress (ES) and to a stress witnessing procedure (SW), as models for physical and psychological stress paradigms, respectively, on phagocytosis and H2O2 production in peritoneal macrophages isolated from Albino Oxford (AO) and Dark Agouti (DA) rats. In addition, we studied the in vitro effects of methionine-enkephalin (ME) on phagocytosis and H2O2 production in peritoneal macrophages isolated from both AO and DA rats that had been exposed to ES and SW procedures. The results showed that peritoneal macrophages isolated from DA rats were less sensitive to the suppressive effects of ES and SW than macrophages isolated from AO rats. In vitro treatment of macrophages isolated from AO rats with ME mimicked to some extent the suppressive effects of ES and SW on phagocytosis and H2O2 production and additionally diminished H2O2 release in macrophages isolated from AO rats previously exposed to ES or SW ME did not have any effect on phagocytosis in macrophages isolated from DA rats, but changed H2O2 production in a concentration-dependent manner. In macrophages isolated from DA rats previously exposed to stress the effect of ME was dependent on the macrophage function tested and the particular stress paradigm employed. Our results emphasise the fact that both beneficial and detrimental effects of stress on immune system functions could be attributed to the individual variations in the macrophage's response to stress mediators. (c) 2006 Elsevier Inc. All rights reserved.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Life Sciences
T1 - The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains
EP - 909
IS - 10
SP - 901
VL - 80
DO - 10.1016/j.lfs.2006.11.019
ER -
@article{
author = "Stanojević, Stanislava and Mitić, Katarina and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana",
year = "2007",
abstract = "The aim of our current study was to investigate the effect of acute exposure to electric tail shock stress (ES) and to a stress witnessing procedure (SW), as models for physical and psychological stress paradigms, respectively, on phagocytosis and H2O2 production in peritoneal macrophages isolated from Albino Oxford (AO) and Dark Agouti (DA) rats. In addition, we studied the in vitro effects of methionine-enkephalin (ME) on phagocytosis and H2O2 production in peritoneal macrophages isolated from both AO and DA rats that had been exposed to ES and SW procedures. The results showed that peritoneal macrophages isolated from DA rats were less sensitive to the suppressive effects of ES and SW than macrophages isolated from AO rats. In vitro treatment of macrophages isolated from AO rats with ME mimicked to some extent the suppressive effects of ES and SW on phagocytosis and H2O2 production and additionally diminished H2O2 release in macrophages isolated from AO rats previously exposed to ES or SW ME did not have any effect on phagocytosis in macrophages isolated from DA rats, but changed H2O2 production in a concentration-dependent manner. In macrophages isolated from DA rats previously exposed to stress the effect of ME was dependent on the macrophage function tested and the particular stress paradigm employed. Our results emphasise the fact that both beneficial and detrimental effects of stress on immune system functions could be attributed to the individual variations in the macrophage's response to stress mediators. (c) 2006 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains",
pages = "909-901",
number = "10",
volume = "80",
doi = "10.1016/j.lfs.2006.11.019"
}
Stanojević, S., Mitić, K., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2007). The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 80(10), 901-909.
https://doi.org/10.1016/j.lfs.2006.11.019
Stanojević S, Mitić K, Vujić V, Kovačević-Jovanović V, Dimitrijević M. The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains. in Life Sciences. 2007;80(10):901-909.
doi:10.1016/j.lfs.2006.11.019 .
Stanojević, Stanislava, Mitić, Katarina, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains" in Life Sciences, 80, no. 10 (2007):901-909,
https://doi.org/10.1016/j.lfs.2006.11.019 . .
