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dc.creatorPolović, Natalija
dc.creatorBlanuša, Milan
dc.creatorGavrović-Jankulović, Marija
dc.creatorAtanasković-Marković, Marina
dc.creatorBurazer, Lidija
dc.creatorJankov, Ratko
dc.creatorVeličković, Tanja
dc.date.accessioned2021-02-18T10:30:08Z
dc.date.available2021-02-18T10:30:08Z
dc.date.issued2007
dc.identifier.issn0954-7894
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/225
dc.description.abstractBackground: It is a general belief that a food allergen should be stable to gastric digestion. Various acidic plant polysaccharides, including pectin, are ubiquitous in fruit matrixes and can form hydrogels under low-pH conditions. Objective: The purpose of this study was to investigate the effect of hydrogel forming polysaccharide-rich fruit matrixes on in vivo gastric and in vitro pepsic digestion of fruit allergens. Methods: Fruit extract proteins (kiwi, banana, apple and cherry) and a purified major kiwi allergen Act c 2 were digested with simulated gastric fluid in accordance with the US Pharmacopeia. In vivo experiments on kiwi fruit digestion were performed on four healthy non-atopic volunteers by examining the gastric content 1 h after ingestion of kiwi fruit. The Act c 2 and kiwi proteins were detected in immunoblots using monoclonal anti-Act c 2 antibodies and rabbit polyclonal antisera. Results: Crude fruit extracts were resistant to digestion by pepsin when compared with commonly prepared extracts. In the gastric content of all volunteers, following kiwi fruit ingestion and immunoblotting, intact Act c 2 was detected with anti-Act c 2 monoclonal antibodies, while kiwi proteins of higher molecular weights were detected using rabbit polyclonal antisera. Addition of apple fruit pectin (1.5% and 3%) to the purified kiwi allergen was able to protect it from pepsin digestion in vitro. Conclusion: The matrix effect in pectin-rich fruits can influence the digestibility of food proteins and thereby the process of allergic sensitization in atopic individuals.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142020/RS//
dc.rightsrestrictedAccess
dc.sourceClinical and Experimental Allergy
dc.subjectAct c 2en
dc.subjectDigestibility assayen
dc.subjectFood allergyen
dc.subjectMatrix effecten
dc.subjectPectinen
dc.subjectThaumatin-like proteinen
dc.titleA matrix effect in pectin-rich fruits hampers digestion of allergen by pepsin in vivo and in vitroen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage771
dc.citation.issue5
dc.citation.other37(5): 764-771
dc.citation.rankM21
dc.citation.spage764
dc.citation.volume37
dc.identifier.doi10.1111/j.1365-2222.2007.02703.x
dc.identifier.pmid17456224
dc.identifier.rcubconv_508
dc.identifier.scopus2-s2.0-34247376603
dc.identifier.wos000245939700016
dc.type.versionpublishedVersion


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