Beta-endorphin differentially affects inflammation in two inbred rat strains
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2006
Authors
Stanojević, StanislavaMitić, Katarina
Vujić, Vesna
Kovačević-Jovanović, Vesna
Dimitrijević, Mirjana
Article (Published version)
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Show full item recordAbstract
It has been shown that inflammation of rat paws elicits accumulation of opioid peptide is-endorphin-containing immune cells in the inflamed subcutaneous tissue, contributing to immumocyte-produced pain suppression. However, the possible mechanisms involved in the pharmacological application of beta-endorphin in rat paw inflammation have not been investigated. The present study was set up to explore the effects of intraplantar injection of beta-endorphin on Concanavalin A-induced paw edema in two inbred rat strains, Albino Oxford (AO) and Dark Agouti (DA). Both high dose-induced suppression and low dose-induced potentiation of edema development in AO and DA rats, respectively, were blocked with antagonists specific for 6 (naltrindole) and K (nor-binaltorphimine) opioid receptors. beta-endorphin in vitro decreased phagocytosis and increased nitric oxide (NO) production in air pouch granulocytes obtained from AD rats. However, in cells from DA rat strain beta-endorphin modulated both phag...ocytosis and NO production in a concentration-dependent manner. It could be concluded that the strain-dependent opposing effects of endorphin on paw inflammation are mediated through 6 and K opioid receptors and probably involve changes in the production of reactive oxygen species by inflammatory cells. Our results point to the importance of genotype for pharmacological manipulations and the development of inflammation. (c) 2006 Elsevier B.V. All rights reserved.
Keywords:
paw edema / beta-endorphin / granulocytes / phagocytosis / NO (nitric oxide) production / mu, delta, kappa opioid receptors / AO (Albino Oxford) rat / DA (Dark Agouti) ratSource:
European Journal of Pharmacology, 2006, 549, 1-3, 157-165Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Neuroendokrina modulacija imunskog odgovora: uloga simpato-adrenomedularnog sistema (RS-MESTD-MPN2006-2010-145049)
DOI: 10.1016/j.ejphar.2006.08.012
ISSN: 0014-2999
PubMed: 16978600
WoS: 000241225700021
Scopus: 2-s2.0-33748961109
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TorlakTY - JOUR AU - Stanojević, Stanislava AU - Mitić, Katarina AU - Vujić, Vesna AU - Kovačević-Jovanović, Vesna AU - Dimitrijević, Mirjana PY - 2006 UR - http://intor.torlakinstitut.com/handle/123456789/212 AB - It has been shown that inflammation of rat paws elicits accumulation of opioid peptide is-endorphin-containing immune cells in the inflamed subcutaneous tissue, contributing to immumocyte-produced pain suppression. However, the possible mechanisms involved in the pharmacological application of beta-endorphin in rat paw inflammation have not been investigated. The present study was set up to explore the effects of intraplantar injection of beta-endorphin on Concanavalin A-induced paw edema in two inbred rat strains, Albino Oxford (AO) and Dark Agouti (DA). Both high dose-induced suppression and low dose-induced potentiation of edema development in AO and DA rats, respectively, were blocked with antagonists specific for 6 (naltrindole) and K (nor-binaltorphimine) opioid receptors. beta-endorphin in vitro decreased phagocytosis and increased nitric oxide (NO) production in air pouch granulocytes obtained from AD rats. However, in cells from DA rat strain beta-endorphin modulated both phagocytosis and NO production in a concentration-dependent manner. It could be concluded that the strain-dependent opposing effects of endorphin on paw inflammation are mediated through 6 and K opioid receptors and probably involve changes in the production of reactive oxygen species by inflammatory cells. Our results point to the importance of genotype for pharmacological manipulations and the development of inflammation. (c) 2006 Elsevier B.V. All rights reserved. PB - Elsevier, Amsterdam T2 - European Journal of Pharmacology T1 - Beta-endorphin differentially affects inflammation in two inbred rat strains EP - 165 IS - 1-3 SP - 157 VL - 549 DO - 10.1016/j.ejphar.2006.08.012 ER -
@article{ author = "Stanojević, Stanislava and Mitić, Katarina and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana", year = "2006", abstract = "It has been shown that inflammation of rat paws elicits accumulation of opioid peptide is-endorphin-containing immune cells in the inflamed subcutaneous tissue, contributing to immumocyte-produced pain suppression. However, the possible mechanisms involved in the pharmacological application of beta-endorphin in rat paw inflammation have not been investigated. The present study was set up to explore the effects of intraplantar injection of beta-endorphin on Concanavalin A-induced paw edema in two inbred rat strains, Albino Oxford (AO) and Dark Agouti (DA). Both high dose-induced suppression and low dose-induced potentiation of edema development in AO and DA rats, respectively, were blocked with antagonists specific for 6 (naltrindole) and K (nor-binaltorphimine) opioid receptors. beta-endorphin in vitro decreased phagocytosis and increased nitric oxide (NO) production in air pouch granulocytes obtained from AD rats. However, in cells from DA rat strain beta-endorphin modulated both phagocytosis and NO production in a concentration-dependent manner. It could be concluded that the strain-dependent opposing effects of endorphin on paw inflammation are mediated through 6 and K opioid receptors and probably involve changes in the production of reactive oxygen species by inflammatory cells. Our results point to the importance of genotype for pharmacological manipulations and the development of inflammation. (c) 2006 Elsevier B.V. All rights reserved.", publisher = "Elsevier, Amsterdam", journal = "European Journal of Pharmacology", title = "Beta-endorphin differentially affects inflammation in two inbred rat strains", pages = "165-157", number = "1-3", volume = "549", doi = "10.1016/j.ejphar.2006.08.012" }
Stanojević, S., Mitić, K., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2006). Beta-endorphin differentially affects inflammation in two inbred rat strains. in European Journal of Pharmacology Elsevier, Amsterdam., 549(1-3), 157-165. https://doi.org/10.1016/j.ejphar.2006.08.012
Stanojević S, Mitić K, Vujić V, Kovačević-Jovanović V, Dimitrijević M. Beta-endorphin differentially affects inflammation in two inbred rat strains. in European Journal of Pharmacology. 2006;549(1-3):157-165. doi:10.1016/j.ejphar.2006.08.012 .
Stanojević, Stanislava, Mitić, Katarina, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "Beta-endorphin differentially affects inflammation in two inbred rat strains" in European Journal of Pharmacology, 549, no. 1-3 (2006):157-165, https://doi.org/10.1016/j.ejphar.2006.08.012 . .