Neuropeptide Y and its receptor subtypes specifically modulate rat peritoneal macrophage functions in vitro: counter regulation through Y1 and Y2/5 receptors
Само за регистроване кориснике
2005
Аутори
Dimitrijević, MirjanaStanojević, Stanislava
Vujić, Vesna
Beck-Sickinger, A.
von Hoersten, Stephan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
It is well documented that neuropeptide Y (NPY) exerts a wide range of biological functions through at least five NPY Y receptor subtypes (Y1-Y5), but its immunological effects only recently came into focus. Using NPY family pepticles and NPY-related receptor-specific peptides as well as Y1 and Y2 receptor antagonists, we have tested which NPY Y receptors are involved in NPY-induced modulation of rat peritoneal macrophage function in vitro. NPY and PYY increased oxidative burst in phorbol myristate acetate (PMA)-stimulated macrophages involving activation of protein kinase C (PKC), and decreased it in zymosan-stimulated cells resembling inhibition of signaling pathways subsequent to binding of zymosan particles for the iC3b fragment receptor on macrophages. The combined treatment with NPY and NPY Y receptor antagonists revealed that NPY-induced potentiation of oxidative burst in PMA-stimulated cells is mediated through Y1 and Y2 receptors, while NPY-induced suppression in zymosan-stimu...lated cells is mediated through Y2 receptors only. NPY-related peptides differently modulated macrophage function, confirming involvement of NPY Y2 receptor in both potentiation and suppression of oxidative burst in these cells. Additionally, it was shown that NPY Y5 receptor mediated suppression of oxidative burst in PMA- and zymosan-stimulated macrophages. Taken together, the present data reveal an NPY Y1 and Y2/Y5 receptor interaction in NPY-induced modulation of macrophage functions related to inflammation. (C) 2004 Elsevier B.V. All rights reserved.
Кључне речи:
neuropeptide Y (NPY) / NPY receptors / macrophage / oxidative burst / dark agouti (DA) ratsИзвор:
Regulatory Peptides, 2005, 124, 1-3, 163-172Издавач:
- Elsevier, Amsterdam
DOI: 10.1016/j.regpep.2004.07.012
ISSN: 0167-0115
PubMed: 15544855
WoS: 000225638300021
Scopus: 2-s2.0-8444225461
Институција/група
TorlakTY - JOUR AU - Dimitrijević, Mirjana AU - Stanojević, Stanislava AU - Vujić, Vesna AU - Beck-Sickinger, A. AU - von Hoersten, Stephan PY - 2005 UR - http://intor.torlakinstitut.com/handle/123456789/200 AB - It is well documented that neuropeptide Y (NPY) exerts a wide range of biological functions through at least five NPY Y receptor subtypes (Y1-Y5), but its immunological effects only recently came into focus. Using NPY family pepticles and NPY-related receptor-specific peptides as well as Y1 and Y2 receptor antagonists, we have tested which NPY Y receptors are involved in NPY-induced modulation of rat peritoneal macrophage function in vitro. NPY and PYY increased oxidative burst in phorbol myristate acetate (PMA)-stimulated macrophages involving activation of protein kinase C (PKC), and decreased it in zymosan-stimulated cells resembling inhibition of signaling pathways subsequent to binding of zymosan particles for the iC3b fragment receptor on macrophages. The combined treatment with NPY and NPY Y receptor antagonists revealed that NPY-induced potentiation of oxidative burst in PMA-stimulated cells is mediated through Y1 and Y2 receptors, while NPY-induced suppression in zymosan-stimulated cells is mediated through Y2 receptors only. NPY-related peptides differently modulated macrophage function, confirming involvement of NPY Y2 receptor in both potentiation and suppression of oxidative burst in these cells. Additionally, it was shown that NPY Y5 receptor mediated suppression of oxidative burst in PMA- and zymosan-stimulated macrophages. Taken together, the present data reveal an NPY Y1 and Y2/Y5 receptor interaction in NPY-induced modulation of macrophage functions related to inflammation. (C) 2004 Elsevier B.V. All rights reserved. PB - Elsevier, Amsterdam T2 - Regulatory Peptides T1 - Neuropeptide Y and its receptor subtypes specifically modulate rat peritoneal macrophage functions in vitro: counter regulation through Y1 and Y2/5 receptors EP - 172 IS - 1-3 SP - 163 VL - 124 DO - 10.1016/j.regpep.2004.07.012 ER -
@article{ author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Vujić, Vesna and Beck-Sickinger, A. and von Hoersten, Stephan", year = "2005", abstract = "It is well documented that neuropeptide Y (NPY) exerts a wide range of biological functions through at least five NPY Y receptor subtypes (Y1-Y5), but its immunological effects only recently came into focus. Using NPY family pepticles and NPY-related receptor-specific peptides as well as Y1 and Y2 receptor antagonists, we have tested which NPY Y receptors are involved in NPY-induced modulation of rat peritoneal macrophage function in vitro. NPY and PYY increased oxidative burst in phorbol myristate acetate (PMA)-stimulated macrophages involving activation of protein kinase C (PKC), and decreased it in zymosan-stimulated cells resembling inhibition of signaling pathways subsequent to binding of zymosan particles for the iC3b fragment receptor on macrophages. The combined treatment with NPY and NPY Y receptor antagonists revealed that NPY-induced potentiation of oxidative burst in PMA-stimulated cells is mediated through Y1 and Y2 receptors, while NPY-induced suppression in zymosan-stimulated cells is mediated through Y2 receptors only. NPY-related peptides differently modulated macrophage function, confirming involvement of NPY Y2 receptor in both potentiation and suppression of oxidative burst in these cells. Additionally, it was shown that NPY Y5 receptor mediated suppression of oxidative burst in PMA- and zymosan-stimulated macrophages. Taken together, the present data reveal an NPY Y1 and Y2/Y5 receptor interaction in NPY-induced modulation of macrophage functions related to inflammation. (C) 2004 Elsevier B.V. All rights reserved.", publisher = "Elsevier, Amsterdam", journal = "Regulatory Peptides", title = "Neuropeptide Y and its receptor subtypes specifically modulate rat peritoneal macrophage functions in vitro: counter regulation through Y1 and Y2/5 receptors", pages = "172-163", number = "1-3", volume = "124", doi = "10.1016/j.regpep.2004.07.012" }
Dimitrijević, M., Stanojević, S., Vujić, V., Beck-Sickinger, A.,& von Hoersten, S.. (2005). Neuropeptide Y and its receptor subtypes specifically modulate rat peritoneal macrophage functions in vitro: counter regulation through Y1 and Y2/5 receptors. in Regulatory Peptides Elsevier, Amsterdam., 124(1-3), 163-172. https://doi.org/10.1016/j.regpep.2004.07.012
Dimitrijević M, Stanojević S, Vujić V, Beck-Sickinger A, von Hoersten S. Neuropeptide Y and its receptor subtypes specifically modulate rat peritoneal macrophage functions in vitro: counter regulation through Y1 and Y2/5 receptors. in Regulatory Peptides. 2005;124(1-3):163-172. doi:10.1016/j.regpep.2004.07.012 .
Dimitrijević, Mirjana, Stanojević, Stanislava, Vujić, Vesna, Beck-Sickinger, A., von Hoersten, Stephan, "Neuropeptide Y and its receptor subtypes specifically modulate rat peritoneal macrophage functions in vitro: counter regulation through Y1 and Y2/5 receptors" in Regulatory Peptides, 124, no. 1-3 (2005):163-172, https://doi.org/10.1016/j.regpep.2004.07.012 . .