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dc.creatorPlećaš-Solarović, Bosiljka
dc.creatorHristić-Živković, I.
dc.creatorRadojević, Katarina
dc.creatorKosec, Duško
dc.creatorLeposavić, Gordana
dc.date.accessioned2021-02-18T10:27:48Z
dc.date.available2021-02-18T10:27:48Z
dc.date.issued2005
dc.identifier.issn0213-3911
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/191
dc.description.abstractIn order to examine the influence of chronic alpha(1)-adrenergic receptor (alpha(1)-AR) blockade on the thymus structure and T-cell maturation, peripubertal and adult male rats were treated with urapidil (0.20 mg/kg BW/d; s.c.) over 15 consecutive days. Thymic structure and phenotypic characteristics of the thymocytes were assessed by stereological and flow cytometry analysis, respectively. In immature rats, treatment with urapidil reduced the body weight gain and, affecting the volume of cortical compartment and its cellularity decreased the organ size and the total number of thymocytes compared to acre-matched saline-injected controls. The percentage of CD4+8- single positive (SP) thymocytes was decreased, while that of CD4-8+ was increased suggesting, most likely, a disregulation in final steps of the positively selected cells maturation. However alpha(1)-AR blockade in adult rats increased the thymus weight as a consequence of increase in the cortical size and cellularity. The increased percentage of most immature CD4-8- double negative (DN) cells associated with decreased percentage of immature CD4+8+ double positive (DP) thymocytes suggests a decelerated transition from DN to DP stage of T-cell development. As in immature rats, the treatment in adult rats evoked changes in the relative numbers of SP cells, but contrary to immature animals, favoring the maturation of CD4+8- over CD4-8+ thymocytes. These results demonstrate that: i) chronic blockade of alpha(1)-ARs affects both the thymus structure and thymocyte differentiation, ii) these effects are age-dependent, pointing out to pharmacological manipulation of alpha(1)-AR-mediated signaling as potential means for modulation of the intrathymic T-cell maturation.en
dc.publisherF Hernandez, Murcia
dc.rightsrestrictedAccess
dc.sourceHistology and Histopathology
dc.subjectalpha(1)-adrenoceptor blockadeen
dc.subjecturapidilen
dc.subjectraten
dc.subjectthymus structureen
dc.subjectthymocyte developmenten
dc.titleChronic alpha(1)-adrenoreceptor blockade produces age-dependent changes in rat thymus structure and thymocyte differentiationen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage841
dc.citation.issue3
dc.citation.other20(3): 833-841
dc.citation.rankM22
dc.citation.spage833
dc.citation.volume20
dc.identifier.pmid15944933
dc.identifier.rcubconv_175
dc.identifier.scopus2-s2.0-21744442797
dc.identifier.wos000230287100020
dc.type.versionpublishedVersion


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