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Proliferacija naivnih i aktiviranih T limfocita u prisustvu tkivno specifičnih makrofaga

dc.creatorŽivančević-Simonović, Snežana
dc.creatorInić-Kanada, Aleksandra
dc.creatorStojanović, Marijana
dc.creatorDimitrijević, Ljiljana
dc.date.accessioned2021-02-18T10:27:31Z
dc.date.available2021-02-18T10:27:31Z
dc.date.issued2004
dc.identifier.issn0567-8315
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/187
dc.description.abstractIn this study the antigen-presenting ability of tissue specific macrophages isolated from bone marrow, spleen, peritoneal cavity and lungs was analyzed. Murine macrophages were isolated by a one-step adherence procedure (for 24 hours) and pretreated with mytomycin C. The antigen-presenting ability of the macrophages was tested in T cell proliferation assays. The ability of macrophages to support antigenspecific proliferation of T lymphoblasts was investigated when sheep red blood cell (SRBC)-specific T blasts were stimulated in vitro by antigen in the presence of different numbers of tissue specific macrophages. On the other hand, the abilities of macrophages to induce proliferation of naïve T cells were analyzed in allogeneic and syngeneic mixed leukocyte reactions (MLRs). It was demonstrated that tissue specific macrophages supported antigen specific proliferation of T lymphoblasts in vitro. They also induced the activation of allogeneic and syngeneic T cells. Increasing the number of macrophages co-cultured with T cells, led to a certain inhibitory effect on T cell proliferation.en
dc.description.abstractU ovom radu je ispitivana sposobnost mišjih makrofaga izolovanih iz kostne srži, slezine, peritonealne šupljine i alveola da prezentuju antigen i indukuju proliferaciju T limfocita. Makrofazi su izolovani adherencijom tokom 24-časovne kulture i pretretirani mitomicinom C. Da bi se ispitala sposobnost makrofaga da indukuju proliferaciju limfoblasta, T limfociti specifični za ovčje eritrocite izolovani iz limfnih čvorova imunizovanih miševa u in vitro uslovima, su restimulisani antigenom u prisustvu tkivnih makrofaga izolovanih iz različitih tkiva. Pored toga, sposobnost makrofaga da indukuju proliferaciju in vivo naivnih T limfocita ispitivana je u mešanoj kulturi tkivnih makrofaga i alogenih ili singenih limfocita. Dokazano je da tkivni makrofazi izolovani iz kostne srži, slezine, peritonealne šupljine i alveola mogu da potpomognu antigen-specifičnu proliferaciju T limfoblasta u in vitro uslovima, kao i da aktiviraju naivne alogene i singene T limfocite. Povećanje broja makrofaga dovelo je do smanjenja T limfocitne proliferacije.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectmacrophageen
dc.subjectantigen-presenting cellsen
dc.subjectsheep redblood cells (SRBC)en
dc.subjectmixed leukocyte reaction (MLR)en
dc.subjectmouseen
dc.titleProliferation of naive T lymphocytes and T lymphoblasts in the presence of tissue specific macrophagesen
dc.titleProliferacija naivnih i aktiviranih T limfocita u prisustvu tkivno specifičnih makrofagasr
dc.typearticle
dc.rights.licenseARR
dc.citation.epage335
dc.citation.issue5-6
dc.citation.other54(5-6): 327-335
dc.citation.rankM23
dc.citation.spage327
dc.citation.volume54
dc.identifier.doi10.2298/AVB0406327Z
dc.identifier.fulltexthttp://intor.torlakinstitut.com/bitstream/id/61/184.pdf
dc.identifier.rcubconv_50
dc.identifier.scopus2-s2.0-13244297001
dc.identifier.wos000226086100001
dc.type.versionpublishedVersion


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