IgE cross-reactivity between meadow fescue pollen and kiwi fruit in patients' sera with sensitivity to both extracts
Апстракт
Background: The presence of IgE reactivity to kiwi fruit and grass pollen allergens which could be caused by cross-reactivity has been detected in many patients with allergy. Proper identification of allergens as well cross-reactive components is essential for understanding fruit- and pollen-associated hypersensitivity. Methods: Using the sera from the polysensitized patients with specific IgE to grass pollen and kiwi fruit we tested reactivity to both allergen sources. IgE reactivity was exhibited in 8 serum samples by immunoblot. A serum pool formed by 8 individual sera was used for the investigation of IgE crossreactivity. SDS-PAGE immunoblot-inhibition assay was performed, by preincubation of the sera with meadow fescue pollen, kiwi fruit extract, and isolated 24 kDa kiwi protein. To determine the allergens of kiwi fruit extract, we performed 2D PAGE immunoblot. In order to detect the crossreactive components between two allergen sources, a specific IgE for the 24 kDa kiwi allergen... was purified. Results: SDS-PAGE immunoblot meadow fescue pollen showed allergens ranging from 94 to 16 kDa, and kiwi-fruit had 12 allergens ranging from 94 to 17 kDa. 2D-PAGE analysis revealed at least 15 spots in the kiwi extract and about 10 allergens. The most prominent allergen in 2D PAGE immunoblot was protein with 24 kDa and pI 9.4-9.5. Using an affinity-purified specific IgE we found that the 24 kDa kiwi allergen shared IgE-reactive epitopes with the meadow fescue group 4 and allergen about 36 kDa. Crossreactivity between isolated 24 kDa kiwi allergen and Fes p 4 was confirmed by anti-grass group 4 moab 2D8. Conclusion: Our findings showed that fescue meadow pollen cross-sensitize to kiwi fruits. A 24 kDa kiwi glycoprotein represent potential major allergen, which share common epitopes with Fes p 4 and 36 kDa meadow fescue allergen.
Кључне речи:
Food allergy / Grass pollen allergy / IgE crossreactivity / Kiwi allergens / Meadow grass / Monoclonal antibodyИзвор:
Journal of Investigational Allergology and Clinical Immunology, 2002, 12, 4, 279-286Издавач:
- Hogefre and Huber Publishers
Институција/група
TorlakTY - JOUR AU - Gavrović-Jankulović, Marija AU - Ćirković, Tanja AU - Burazer, Lidija AU - Vučković, Olga AU - Jankov, Ratko PY - 2002 UR - http://intor.torlakinstitut.com/handle/123456789/145 AB - Background: The presence of IgE reactivity to kiwi fruit and grass pollen allergens which could be caused by cross-reactivity has been detected in many patients with allergy. Proper identification of allergens as well cross-reactive components is essential for understanding fruit- and pollen-associated hypersensitivity. Methods: Using the sera from the polysensitized patients with specific IgE to grass pollen and kiwi fruit we tested reactivity to both allergen sources. IgE reactivity was exhibited in 8 serum samples by immunoblot. A serum pool formed by 8 individual sera was used for the investigation of IgE crossreactivity. SDS-PAGE immunoblot-inhibition assay was performed, by preincubation of the sera with meadow fescue pollen, kiwi fruit extract, and isolated 24 kDa kiwi protein. To determine the allergens of kiwi fruit extract, we performed 2D PAGE immunoblot. In order to detect the crossreactive components between two allergen sources, a specific IgE for the 24 kDa kiwi allergen was purified. Results: SDS-PAGE immunoblot meadow fescue pollen showed allergens ranging from 94 to 16 kDa, and kiwi-fruit had 12 allergens ranging from 94 to 17 kDa. 2D-PAGE analysis revealed at least 15 spots in the kiwi extract and about 10 allergens. The most prominent allergen in 2D PAGE immunoblot was protein with 24 kDa and pI 9.4-9.5. Using an affinity-purified specific IgE we found that the 24 kDa kiwi allergen shared IgE-reactive epitopes with the meadow fescue group 4 and allergen about 36 kDa. Crossreactivity between isolated 24 kDa kiwi allergen and Fes p 4 was confirmed by anti-grass group 4 moab 2D8. Conclusion: Our findings showed that fescue meadow pollen cross-sensitize to kiwi fruits. A 24 kDa kiwi glycoprotein represent potential major allergen, which share common epitopes with Fes p 4 and 36 kDa meadow fescue allergen. PB - Hogefre and Huber Publishers T2 - Journal of Investigational Allergology and Clinical Immunology T1 - IgE cross-reactivity between meadow fescue pollen and kiwi fruit in patients' sera with sensitivity to both extracts EP - 286 IS - 4 SP - 279 VL - 12 UR - https://hdl.handle.net/21.15107/rcub_intor_145 ER -
@article{ author = "Gavrović-Jankulović, Marija and Ćirković, Tanja and Burazer, Lidija and Vučković, Olga and Jankov, Ratko", year = "2002", abstract = "Background: The presence of IgE reactivity to kiwi fruit and grass pollen allergens which could be caused by cross-reactivity has been detected in many patients with allergy. Proper identification of allergens as well cross-reactive components is essential for understanding fruit- and pollen-associated hypersensitivity. Methods: Using the sera from the polysensitized patients with specific IgE to grass pollen and kiwi fruit we tested reactivity to both allergen sources. IgE reactivity was exhibited in 8 serum samples by immunoblot. A serum pool formed by 8 individual sera was used for the investigation of IgE crossreactivity. SDS-PAGE immunoblot-inhibition assay was performed, by preincubation of the sera with meadow fescue pollen, kiwi fruit extract, and isolated 24 kDa kiwi protein. To determine the allergens of kiwi fruit extract, we performed 2D PAGE immunoblot. In order to detect the crossreactive components between two allergen sources, a specific IgE for the 24 kDa kiwi allergen was purified. Results: SDS-PAGE immunoblot meadow fescue pollen showed allergens ranging from 94 to 16 kDa, and kiwi-fruit had 12 allergens ranging from 94 to 17 kDa. 2D-PAGE analysis revealed at least 15 spots in the kiwi extract and about 10 allergens. The most prominent allergen in 2D PAGE immunoblot was protein with 24 kDa and pI 9.4-9.5. Using an affinity-purified specific IgE we found that the 24 kDa kiwi allergen shared IgE-reactive epitopes with the meadow fescue group 4 and allergen about 36 kDa. Crossreactivity between isolated 24 kDa kiwi allergen and Fes p 4 was confirmed by anti-grass group 4 moab 2D8. Conclusion: Our findings showed that fescue meadow pollen cross-sensitize to kiwi fruits. A 24 kDa kiwi glycoprotein represent potential major allergen, which share common epitopes with Fes p 4 and 36 kDa meadow fescue allergen.", publisher = "Hogefre and Huber Publishers", journal = "Journal of Investigational Allergology and Clinical Immunology", title = "IgE cross-reactivity between meadow fescue pollen and kiwi fruit in patients' sera with sensitivity to both extracts", pages = "286-279", number = "4", volume = "12", url = "https://hdl.handle.net/21.15107/rcub_intor_145" }
Gavrović-Jankulović, M., Ćirković, T., Burazer, L., Vučković, O.,& Jankov, R.. (2002). IgE cross-reactivity between meadow fescue pollen and kiwi fruit in patients' sera with sensitivity to both extracts. in Journal of Investigational Allergology and Clinical Immunology Hogefre and Huber Publishers., 12(4), 279-286. https://hdl.handle.net/21.15107/rcub_intor_145
Gavrović-Jankulović M, Ćirković T, Burazer L, Vučković O, Jankov R. IgE cross-reactivity between meadow fescue pollen and kiwi fruit in patients' sera with sensitivity to both extracts. in Journal of Investigational Allergology and Clinical Immunology. 2002;12(4):279-286. https://hdl.handle.net/21.15107/rcub_intor_145 .
Gavrović-Jankulović, Marija, Ćirković, Tanja, Burazer, Lidija, Vučković, Olga, Jankov, Ratko, "IgE cross-reactivity between meadow fescue pollen and kiwi fruit in patients' sera with sensitivity to both extracts" in Journal of Investigational Allergology and Clinical Immunology, 12, no. 4 (2002):279-286, https://hdl.handle.net/21.15107/rcub_intor_145 .