Age-associated reshaping of the thymocyte differentiation model in male rats
Promene u modelu diferentovanja timocita u mužjaka pacova sa godinama
Апстракт
In order to elucidate the features of putative age-related changes in the intrathymic T cell maturation sequence, the thymus weight, thymocyte yield and relative proportions of thymocyte subsets were analyzed at distinct maturational stages (delineated by analysis of the expression of CD4/CD8 coreceptor molecules and TCR molecular complex) in male AO rats, from the peripubertal period (1 month of age) until 10 months of age. In 2-month-old rats both the thymus weight and lymphoid content were greater than in 1-month-old rats. The values of both parameters in 4-month-old rats were reduced to the corresponding values in 1-month-old rats. These levels were sustained until 10 months of age. However, the thymocyte composition was subjected to substantial changes during the whole period studied, probably due to alterations in action of both extrinsic and intrinsic factors which influence the ability of the thymus to support T cell maturation and/or feedback regulatory action of intrathymic C...D4+ T cells on thymocytopoiesis (between the age of 7 and 10 months).
U cilju definisanja karakteristika procesa sazrevanja T ćelija u timusu u funkciji uzrasta, u mužjaka pacova AO soja, različitog uzrasta (od 1 meseca do 10 meseci) posmatrane su: težina timusa, ukupan broj timocita, kao i relativna zastupljenost pojedinih timocitnih subpopulacija (koje su razdvojene na osnovu ekspresije CD4/8 koreceptornih molekula i antigena TCRαβ molekulskog kompleksa). U životinja uzrasta 2 meseca i težina timusa i ukupan broj timocita bili su značajno veći nego u životinja uzrasta od mesec dana. Međutim, u životinja uzrasta od 4 meseca vrednost ovih parametra nije se razlikovala od vrednosti odgovarajućih parametara sa mesec dana. Vrednosti oba ova parametra nisu se bitno menjale od 4. do 10. meseca života. Sa druge strane, naši rezultati su pokazali značajne promene u relativnom odnosu timocitnih subpopulacija u ispitivanom periodu. Ove promene se mogu objasniti promenama u delovanju, kako spoljašnjih tako i unutrašnjih, faktora koji modulišu sposobnost timusne mi...krosredine da podrži proces sazrevanja T ćelija i/ili utiču na efikasnost regulatornog delovanja intratimusnih CD4+ T ćelija, mehanizmom negativne povratne sprege, na proces sazrevanja T ćelija u timusu.
Кључне речи:
aging / male rats thymus / thymus size / T cell differentiationИзвор:
Acta veterinaria - Beograd, 2001, 51, 4, 203-217Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Институција/група
TorlakTY - JOUR AU - Leposavić, Gordana AU - Pejčić-Karapetrović, Branka AU - Kosec, Duško AU - Vidić-Danković, Biljana PY - 2001 UR - http://intor.torlakinstitut.com/handle/123456789/130 AB - In order to elucidate the features of putative age-related changes in the intrathymic T cell maturation sequence, the thymus weight, thymocyte yield and relative proportions of thymocyte subsets were analyzed at distinct maturational stages (delineated by analysis of the expression of CD4/CD8 coreceptor molecules and TCR molecular complex) in male AO rats, from the peripubertal period (1 month of age) until 10 months of age. In 2-month-old rats both the thymus weight and lymphoid content were greater than in 1-month-old rats. The values of both parameters in 4-month-old rats were reduced to the corresponding values in 1-month-old rats. These levels were sustained until 10 months of age. However, the thymocyte composition was subjected to substantial changes during the whole period studied, probably due to alterations in action of both extrinsic and intrinsic factors which influence the ability of the thymus to support T cell maturation and/or feedback regulatory action of intrathymic CD4+ T cells on thymocytopoiesis (between the age of 7 and 10 months). AB - U cilju definisanja karakteristika procesa sazrevanja T ćelija u timusu u funkciji uzrasta, u mužjaka pacova AO soja, različitog uzrasta (od 1 meseca do 10 meseci) posmatrane su: težina timusa, ukupan broj timocita, kao i relativna zastupljenost pojedinih timocitnih subpopulacija (koje su razdvojene na osnovu ekspresije CD4/8 koreceptornih molekula i antigena TCRαβ molekulskog kompleksa). U životinja uzrasta 2 meseca i težina timusa i ukupan broj timocita bili su značajno veći nego u životinja uzrasta od mesec dana. Međutim, u životinja uzrasta od 4 meseca vrednost ovih parametra nije se razlikovala od vrednosti odgovarajućih parametara sa mesec dana. Vrednosti oba ova parametra nisu se bitno menjale od 4. do 10. meseca života. Sa druge strane, naši rezultati su pokazali značajne promene u relativnom odnosu timocitnih subpopulacija u ispitivanom periodu. Ove promene se mogu objasniti promenama u delovanju, kako spoljašnjih tako i unutrašnjih, faktora koji modulišu sposobnost timusne mikrosredine da podrži proces sazrevanja T ćelija i/ili utiču na efikasnost regulatornog delovanja intratimusnih CD4+ T ćelija, mehanizmom negativne povratne sprege, na proces sazrevanja T ćelija u timusu. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta veterinaria - Beograd T1 - Age-associated reshaping of the thymocyte differentiation model in male rats T1 - Promene u modelu diferentovanja timocita u mužjaka pacova sa godinama EP - 217 IS - 4 SP - 203 VL - 51 UR - https://hdl.handle.net/21.15107/rcub_intor_130 ER -
@article{ author = "Leposavić, Gordana and Pejčić-Karapetrović, Branka and Kosec, Duško and Vidić-Danković, Biljana", year = "2001", abstract = "In order to elucidate the features of putative age-related changes in the intrathymic T cell maturation sequence, the thymus weight, thymocyte yield and relative proportions of thymocyte subsets were analyzed at distinct maturational stages (delineated by analysis of the expression of CD4/CD8 coreceptor molecules and TCR molecular complex) in male AO rats, from the peripubertal period (1 month of age) until 10 months of age. In 2-month-old rats both the thymus weight and lymphoid content were greater than in 1-month-old rats. The values of both parameters in 4-month-old rats were reduced to the corresponding values in 1-month-old rats. These levels were sustained until 10 months of age. However, the thymocyte composition was subjected to substantial changes during the whole period studied, probably due to alterations in action of both extrinsic and intrinsic factors which influence the ability of the thymus to support T cell maturation and/or feedback regulatory action of intrathymic CD4+ T cells on thymocytopoiesis (between the age of 7 and 10 months)., U cilju definisanja karakteristika procesa sazrevanja T ćelija u timusu u funkciji uzrasta, u mužjaka pacova AO soja, različitog uzrasta (od 1 meseca do 10 meseci) posmatrane su: težina timusa, ukupan broj timocita, kao i relativna zastupljenost pojedinih timocitnih subpopulacija (koje su razdvojene na osnovu ekspresije CD4/8 koreceptornih molekula i antigena TCRαβ molekulskog kompleksa). U životinja uzrasta 2 meseca i težina timusa i ukupan broj timocita bili su značajno veći nego u životinja uzrasta od mesec dana. Međutim, u životinja uzrasta od 4 meseca vrednost ovih parametra nije se razlikovala od vrednosti odgovarajućih parametara sa mesec dana. Vrednosti oba ova parametra nisu se bitno menjale od 4. do 10. meseca života. Sa druge strane, naši rezultati su pokazali značajne promene u relativnom odnosu timocitnih subpopulacija u ispitivanom periodu. Ove promene se mogu objasniti promenama u delovanju, kako spoljašnjih tako i unutrašnjih, faktora koji modulišu sposobnost timusne mikrosredine da podrži proces sazrevanja T ćelija i/ili utiču na efikasnost regulatornog delovanja intratimusnih CD4+ T ćelija, mehanizmom negativne povratne sprege, na proces sazrevanja T ćelija u timusu.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta veterinaria - Beograd", title = "Age-associated reshaping of the thymocyte differentiation model in male rats, Promene u modelu diferentovanja timocita u mužjaka pacova sa godinama", pages = "217-203", number = "4", volume = "51", url = "https://hdl.handle.net/21.15107/rcub_intor_130" }
Leposavić, G., Pejčić-Karapetrović, B., Kosec, D.,& Vidić-Danković, B.. (2001). Age-associated reshaping of the thymocyte differentiation model in male rats. in Acta veterinaria - Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 51(4), 203-217. https://hdl.handle.net/21.15107/rcub_intor_130
Leposavić G, Pejčić-Karapetrović B, Kosec D, Vidić-Danković B. Age-associated reshaping of the thymocyte differentiation model in male rats. in Acta veterinaria - Beograd. 2001;51(4):203-217. https://hdl.handle.net/21.15107/rcub_intor_130 .
Leposavić, Gordana, Pejčić-Karapetrović, Branka, Kosec, Duško, Vidić-Danković, Biljana, "Age-associated reshaping of the thymocyte differentiation model in male rats" in Acta veterinaria - Beograd, 51, no. 4 (2001):203-217, https://hdl.handle.net/21.15107/rcub_intor_130 .