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In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones

Authorized Users Only
2001
Authors
Leposavić, Gordana
Obradović, S.
Kosec, Duško
Pejčić-Karapetrović, Branka
Vidić-Danković, Biljana
Article (Published version)
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Abstract
This study examined the effects of the principal ovarian steroids, 17β-estradiol (E) and progesterone (P), on the thymic structure and on the intrathymic development of T-cells. Adult female rats were ovariectomized (OVX) and treated for 14 days with physiological doses of either E or P; controls received an equivalent volume of vehicle. Ovariectomy produced a marked increase (vs. sham-operated controls) in thymus weight, which was associated with an increase in the volume and cellularity of both the medulla and cortex. Treatment of OVX rats with E reduced the thymic weight to value, which was significantly lower than that of sham-operated controls decreasing the volume of cortex below level in sham-OVX rats, and reversing the effect of ovariectomy on the volume of medulla. P only prevented the increases in thymus weight and cortical volume induced by OVX. However, unlike E, it had no discernable effect on the medullary volume. E treatment reduced the cellularity of the cortex and medu...lla to values, which were lower than those of sham-OVX rats, while P only reversed the effects of OVX on the cellularity of both the compartments. Ovariectomy also had a profound effect on the thymocyte profile, increasing the proportion of CD4 + 8 + TCRαβ - cells and producing a corresponding decrease in the relative proportions of all TCR αβ high cell subsets. The decrease in the latter was opposed by treatment with E or P. However, the sensitivity of the less mature cells (except CD4 - 8 - TCR αβ - , the percentage of which was reduced by both hormones) to the two hormones differed. E reduced the relative proportion of CD4 - 8 + TCRαβ - , CD4 - 8 + TCRαβ low and CD4 + 8 + TCRαβ - cells, while P increased the percentage of CD4 - 8 + TCRαβ low cells. The results suggest that E and P affect both the lymphoid and nonlymphoid compartments of the thymus, and that while P increases the volume of the nonlymphoid component of the medulla, E has the opposite effect. The finding that ovariectomy decreased while E and P increased the relative proportion of the most mature thymocytes, which include CD4 - 8 - TCRαβ high cells that are believed to harbour potentially autoreactive cell clones, is particularly interesting and may relate to the high propensity of autoimmune diseases in females.

Keywords:
Estrogens / Ovariectomy / Progesterone / Rat thymus / T-cell maturation
Source:
International Immunopharmacology, 2001, 1, 1, 1-12
Publisher:
  • Elsevier

DOI: 10.1016/S1567-5769(00)00006-0

ISSN: 1567-5769

PubMed: 11367507

WoS: 000168328700001

Scopus: 2-s2.0-0035127781
[ Google Scholar ]
23
21
URI
http://intor.torlakinstitut.com/handle/123456789/129
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Torlak
TY  - JOUR
AU  - Leposavić, Gordana
AU  - Obradović, S.
AU  - Kosec, Duško
AU  - Pejčić-Karapetrović, Branka
AU  - Vidić-Danković, Biljana
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/129
AB  - This study examined the effects of the principal ovarian steroids, 17β-estradiol (E) and progesterone (P), on the thymic structure and on the intrathymic development of T-cells. Adult female rats were ovariectomized (OVX) and treated for 14 days with physiological doses of either E or P; controls received an equivalent volume of vehicle. Ovariectomy produced a marked increase (vs. sham-operated controls) in thymus weight, which was associated with an increase in the volume and cellularity of both the medulla and cortex. Treatment of OVX rats with E reduced the thymic weight to value, which was significantly lower than that of sham-operated controls decreasing the volume of cortex below level in sham-OVX rats, and reversing the effect of ovariectomy on the volume of medulla. P only prevented the increases in thymus weight and cortical volume induced by OVX. However, unlike E, it had no discernable effect on the medullary volume. E treatment reduced the cellularity of the cortex and medulla to values, which were lower than those of sham-OVX rats, while P only reversed the effects of OVX on the cellularity of both the compartments. Ovariectomy also had a profound effect on the thymocyte profile, increasing the proportion of CD4 + 8 + TCRαβ - cells and producing a corresponding decrease in the relative proportions of all TCR αβ high cell subsets. The decrease in the latter was opposed by treatment with E or P. However, the sensitivity of the less mature cells (except CD4 - 8 - TCR αβ - , the percentage of which was reduced by both hormones) to the two hormones differed. E reduced the relative proportion of CD4 - 8 + TCRαβ - , CD4 - 8 + TCRαβ low and CD4 + 8 + TCRαβ - cells, while P increased the percentage of CD4 - 8 + TCRαβ low cells. The results suggest that E and P affect both the lymphoid and nonlymphoid compartments of the thymus, and that while P increases the volume of the nonlymphoid component of the medulla, E has the opposite effect. The finding that ovariectomy decreased while E and P increased the relative proportion of the most mature thymocytes, which include CD4 - 8 - TCRαβ high cells that are believed to harbour potentially autoreactive cell clones, is particularly interesting and may relate to the high propensity of autoimmune diseases in females.
PB  - Elsevier
T2  - International Immunopharmacology
T1  - In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones
EP  - 12
IS  - 1
SP  - 1
VL  - 1
DO  - 10.1016/S1567-5769(00)00006-0
ER  - 
@article{
author = "Leposavić, Gordana and Obradović, S. and Kosec, Duško and Pejčić-Karapetrović, Branka and Vidić-Danković, Biljana",
year = "2001",
abstract = "This study examined the effects of the principal ovarian steroids, 17β-estradiol (E) and progesterone (P), on the thymic structure and on the intrathymic development of T-cells. Adult female rats were ovariectomized (OVX) and treated for 14 days with physiological doses of either E or P; controls received an equivalent volume of vehicle. Ovariectomy produced a marked increase (vs. sham-operated controls) in thymus weight, which was associated with an increase in the volume and cellularity of both the medulla and cortex. Treatment of OVX rats with E reduced the thymic weight to value, which was significantly lower than that of sham-operated controls decreasing the volume of cortex below level in sham-OVX rats, and reversing the effect of ovariectomy on the volume of medulla. P only prevented the increases in thymus weight and cortical volume induced by OVX. However, unlike E, it had no discernable effect on the medullary volume. E treatment reduced the cellularity of the cortex and medulla to values, which were lower than those of sham-OVX rats, while P only reversed the effects of OVX on the cellularity of both the compartments. Ovariectomy also had a profound effect on the thymocyte profile, increasing the proportion of CD4 + 8 + TCRαβ - cells and producing a corresponding decrease in the relative proportions of all TCR αβ high cell subsets. The decrease in the latter was opposed by treatment with E or P. However, the sensitivity of the less mature cells (except CD4 - 8 - TCR αβ - , the percentage of which was reduced by both hormones) to the two hormones differed. E reduced the relative proportion of CD4 - 8 + TCRαβ - , CD4 - 8 + TCRαβ low and CD4 + 8 + TCRαβ - cells, while P increased the percentage of CD4 - 8 + TCRαβ low cells. The results suggest that E and P affect both the lymphoid and nonlymphoid compartments of the thymus, and that while P increases the volume of the nonlymphoid component of the medulla, E has the opposite effect. The finding that ovariectomy decreased while E and P increased the relative proportion of the most mature thymocytes, which include CD4 - 8 - TCRαβ high cells that are believed to harbour potentially autoreactive cell clones, is particularly interesting and may relate to the high propensity of autoimmune diseases in females.",
publisher = "Elsevier",
journal = "International Immunopharmacology",
title = "In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones",
pages = "12-1",
number = "1",
volume = "1",
doi = "10.1016/S1567-5769(00)00006-0"
}
Leposavić, G., Obradović, S., Kosec, D., Pejčić-Karapetrović, B.,& Vidić-Danković, B.. (2001). In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones. in International Immunopharmacology
Elsevier., 1(1), 1-12.
https://doi.org/10.1016/S1567-5769(00)00006-0
Leposavić G, Obradović S, Kosec D, Pejčić-Karapetrović B, Vidić-Danković B. In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones. in International Immunopharmacology. 2001;1(1):1-12.
doi:10.1016/S1567-5769(00)00006-0 .
Leposavić, Gordana, Obradović, S., Kosec, Duško, Pejčić-Karapetrović, Branka, Vidić-Danković, Biljana, "In vivo modulation of the distribution of thymocyte subsets by female sex steroid hormones" in International Immunopharmacology, 1, no. 1 (2001):1-12,
https://doi.org/10.1016/S1567-5769(00)00006-0 . .

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