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dc.creatorDimitrijević, Ljiljana
dc.creatorRadulović, M.I.
dc.creatorĆirić, B.P.
dc.creatorPetrićević, Marijana
dc.creatorInić, Aleksandra
dc.creatorNikolić, D.
dc.creatorApostolski, Slobodan
dc.date.accessioned2021-02-18T10:22:19Z
dc.date.available2021-02-18T10:22:19Z
dc.date.issued1999
dc.identifier.issn1093-2607
dc.identifier.urihttp://intor.torlakinstitut.com/handle/123456789/111
dc.description.abstractIn this study we tried to elucidate further the crossreactivity pattern and binding characteristics of human monoclonal IgM DJ which is an anti-DNA antibody and possesses Y7 natural idiotope. Isolated IgM DJ and its enzymatically obtained fragments Fab' and (Fab')2 were tested for binding to more than 26 antigens and nine bacteria in indirect ELISA. Inhibition of binding studies and examination of the stability of antigen-antibody complexes were also done in ELISA assay. IgM DJ bound to single stranded DNA and human lactic acid bacteria, such as L. acidophyllus, B. bifidum and L. plantarum. This binding was shown to be mediated through IgM DJ Fab' fragment. High avidity and low affinity of interactions was estimated from the binding curves of Fab', (Fab')2 fragments and whole IgM. The common epitopic motif on both antigens were negatively charged phosphodiester moieties. Complexes formed with ssDNA and B. bifidum were resistant to washing with high salt. This suggested that electrostatic attraction was not a strong component of the binding. A novel pattern of natural autoantibody reactivity in a human system related to cross-reactivity with DNA and LAB is described. Possible involvement of LAB in induction of natural anti-DNA antibodies is discussed.en
dc.publisherIOS Press
dc.rightsrestrictedAccess
dc.sourceHuman Antibodies
dc.subjectAnti-ssDNA antibodyen
dc.subjectHuman monoclonal IgMen
dc.subjectImmunityen
dc.subjectLactic acid bacteriaen
dc.subjectNatural autoantibodyen
dc.titleHuman monoclonal IgM DJ binds to ssDNA and human commensal bacteriaen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage45
dc.citation.issue1
dc.citation.other9(1): 37-45
dc.citation.spage37
dc.citation.volume9
dc.identifier.doi10.3233/hab-1999-9104
dc.identifier.pmid10331184
dc.identifier.rcubconv_532
dc.identifier.scopus2-s2.0-0033368919
dc.type.versionpublishedVersion


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