Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins
Само за регистроване кориснике
1999
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Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic va...lues. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy.
Кључне речи:
allergoid / chemical modification / Dactylis glomerata / maleic anhydride / orchard grass / skin prick test / succinic anhydrideИзвор:
Allergy, 1999, 54, 2, 128-134Издавач:
- Wiley, Hoboken
DOI: 10.1034/j.1398-9995.1999.00778.x
ISSN: 0105-4538
PubMed: 10221435
WoS: 000079413400005
Scopus: 2-s2.0-0000670417
Институција/група
TorlakTY - JOUR AU - Ćirković, Tanja AU - Bukilica, Mirjana AU - Gavrović, Marija AU - Vujčić, Zoran AU - Petrović, S. AU - Jankov, Ratko PY - 1999 UR - http://intor.torlakinstitut.com/handle/123456789/104 AB - Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic values. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy. PB - Wiley, Hoboken T2 - Allergy T1 - Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins EP - 134 IS - 2 SP - 128 VL - 54 DO - 10.1034/j.1398-9995.1999.00778.x ER -
@article{ author = "Ćirković, Tanja and Bukilica, Mirjana and Gavrović, Marija and Vujčić, Zoran and Petrović, S. and Jankov, Ratko", year = "1999", abstract = "Background: Orchard grass (Dactylis glomerata) pollen proteins were chemically modified by means of acid anhydrides (maleic and succinic anhydride) to obtain low-molecular-weight allergoids. Chemical modification in both cases led to the replacement of one positive charge (epsilon amino group of Lys) by one negative charge, yielding proteins with changed physicochemical properties in comparison to the native orchard grass-pollen proteins. Methods: Physicochemical characterization of derivatives was done by gel chromatography, SDS-PAGE, and isoelectric focusing. To examine the IgE-binding properties of these derivatives, we carried out immunoblotting. To examine the ability of derivatives to induce IgG production, we immunized rabbits. Skin prick testing with the allergoids was performed on 15 individuals allergic to orchard grass pollens and on two healthy subjects. Results: II was shown that the modified proteins retain their original molecular weights, but change pi to more acidic values. In the case of allergoids, a strong reduction in IgE binding was found. Immunization of rabbits with allergoids showed that the derivatives retain the ability to induce IgG production, and that the antisera obtained in such a way react to native (unmodified) extract. The ability of derivatives to induce allergic reaction was significantly reduced. The patients (86.6%) included in our study exhibited less than 50% of native extract response. Among them, 53.3% had no response to one or both allergoids. Conclusions: These modification procedures yield allergoids with a reduced allergenic activity and preserved immunogenic potential suitable for use in immunotherapy.", publisher = "Wiley, Hoboken", journal = "Allergy", title = "Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins", pages = "134-128", number = "2", volume = "54", doi = "10.1034/j.1398-9995.1999.00778.x" }
Ćirković, T., Bukilica, M., Gavrović, M., Vujčić, Z., Petrović, S.,& Jankov, R.. (1999). Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins. in Allergy Wiley, Hoboken., 54(2), 128-134. https://doi.org/10.1034/j.1398-9995.1999.00778.x
Ćirković T, Bukilica M, Gavrović M, Vujčić Z, Petrović S, Jankov R. Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins. in Allergy. 1999;54(2):128-134. doi:10.1034/j.1398-9995.1999.00778.x .
Ćirković, Tanja, Bukilica, Mirjana, Gavrović, Marija, Vujčić, Zoran, Petrović, S., Jankov, Ratko, "Physicochemical and immunologic characterization of low molecular-weight allergoids of Dactylis glomerata pollen proteins" in Allergy, 54, no. 2 (1999):128-134, https://doi.org/10.1034/j.1398-9995.1999.00778.x . .