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Cytomegalovirus infection may be oncoprotective against neoplasms of B-lymphocyte lineage: single-institution experience and survey of global evidence

Janković, Marko; Knežević, Aleksandra; Todorović, Milena; Đunić, Irena; Mihaljević, Biljana; Soldatović, Ivan; Protić, Jelena; Miković, Nevenka; Stoiljković, Vera; Jovanović, Tanja

(BMC, 2022)

TY  - JOUR
AU  - Janković, Marko
AU  - Knežević, Aleksandra
AU  - Todorović, Milena
AU  - Đunić, Irena
AU  - Mihaljević, Biljana
AU  - Soldatović, Ivan
AU  - Protić, Jelena
AU  - Miković, Nevenka
AU  - Stoiljković, Vera
AU  - Jovanović, Tanja
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/629
AB  - Although cytomegalovirus (CMV) is not considered tumorigenic, there is evidence for its oncomodulatory effects and association with hematological neoplasms. Conversely, a number of experimental and clinical studies suggest its putative anti-tumour effect. We investigated the potential connection between chronic CMV infection in patients with B-lymphocyte (B-cell) malignancies in a retrospective single-center study and extracted relevant data on CMV prevalences and the incidences of B-cell cancers the world over.
PB  - BMC
T2  - Virology Journal
T1  - Cytomegalovirus infection may be oncoprotective against neoplasms of B-lymphocyte lineage: single-institution experience and survey of global evidence
IS  - 1
SP  - 155
VL  - 19
DO  - 10.1186/s12985-022-01884-1
ER  - 
@article{
author = "Janković, Marko and Knežević, Aleksandra and Todorović, Milena and Đunić, Irena and Mihaljević, Biljana and Soldatović, Ivan and Protić, Jelena and Miković, Nevenka and Stoiljković, Vera and Jovanović, Tanja",
year = "2022",
abstract = "Although cytomegalovirus (CMV) is not considered tumorigenic, there is evidence for its oncomodulatory effects and association with hematological neoplasms. Conversely, a number of experimental and clinical studies suggest its putative anti-tumour effect. We investigated the potential connection between chronic CMV infection in patients with B-lymphocyte (B-cell) malignancies in a retrospective single-center study and extracted relevant data on CMV prevalences and the incidences of B-cell cancers the world over.",
publisher = "BMC",
journal = "Virology Journal",
title = "Cytomegalovirus infection may be oncoprotective against neoplasms of B-lymphocyte lineage: single-institution experience and survey of global evidence",
number = "1",
pages = "155",
volume = "19",
doi = "10.1186/s12985-022-01884-1"
}
Janković, M., Knežević, A., Todorović, M., Đunić, I., Mihaljević, B., Soldatović, I., Protić, J., Miković, N., Stoiljković, V.,& Jovanović, T.. (2022). Cytomegalovirus infection may be oncoprotective against neoplasms of B-lymphocyte lineage: single-institution experience and survey of global evidence. in Virology Journal
BMC., 19(1), 155.
https://doi.org/10.1186/s12985-022-01884-1
Janković M, Knežević A, Todorović M, Đunić I, Mihaljević B, Soldatović I, Protić J, Miković N, Stoiljković V, Jovanović T. Cytomegalovirus infection may be oncoprotective against neoplasms of B-lymphocyte lineage: single-institution experience and survey of global evidence. in Virology Journal. 2022;19(1):155.
doi:10.1186/s12985-022-01884-1 .
Janković, Marko, Knežević, Aleksandra, Todorović, Milena, Đunić, Irena, Mihaljević, Biljana, Soldatović, Ivan, Protić, Jelena, Miković, Nevenka, Stoiljković, Vera, Jovanović, Tanja, "Cytomegalovirus infection may be oncoprotective against neoplasms of B-lymphocyte lineage: single-institution experience and survey of global evidence" in Virology Journal, 19, no. 1 (2022):155,
https://doi.org/10.1186/s12985-022-01884-1 . .

β-Adrenoceptor Blockade Moderates Neuroinflammation in Male and Female EAE Rats and Abrogates Sexual Dimorphisms in the Major Neuroinflammatory Pathways by Being More Efficient in Males

Pilipović, Ivan; Stojić-Vukanić, Zorica; Prijić, Ivana; Jasnić, Nebojša; Đorđević, Jelena; Leposavić, Gordana

(Springer, 2022)

TY  - JOUR
AU  - Pilipović, Ivan
AU  - Stojić-Vukanić, Zorica
AU  - Prijić, Ivana
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena
AU  - Leposavić, Gordana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4198
UR  - http://intor.torlakinstitut.com/handle/123456789/622
AB  - Our previous studies showed more severe experimental autoimmune encephalomyelitis (EAE) in male compared with female adult rats, and moderating effect of propranolol-induced β-adrenoceptor blockade on EAE in females, the effect associated with transcriptional stimulation of Nrf2/HO-1 axis in spinal cord microglia. This study examined putative sexual dimor- phism in propranolol action on EAE severity. Propranolol treatment beginning from the onset of clinical EAE mitigated EAE severity in rats of both sexes, but to a greater extent in males exhibiting higher noradrenaline levels and myeloid cell β 2 -adrenoceptor expression in spinal cord. This correlated with more prominent stimulatory effects of propranolol not only on CX3CL1/CX3CR1/Nrf2/HO-1 cascade, but also on Stat3/Socs3 signaling axis in spinal cord microglia/myeloid cells (mirrored in the decreased Stat3 and the increased Socs3 expression) from male rats compared with their female counterparts. Propranolol diminished the frequency of activated cells among microglia, increased their phagocyting/endocyting capacity, and shifted cytokine secretory profile of microglia/blood-borne myeloid cells towards an anti-inflammatory/neuroprotective phenotype. Additionally, it downregulated the expression of chemokines (CCL2, CCL19/21) driving T-cell/monocyte traf- ficking into spinal cord. Consequently, in propranolol-treated rats fewer activated CD4+ T cells and IL-17+ T cells, including CD4+IL17+ cells coexpressing IFN-γ/GM-CSF, were recovered from spinal cord of propranolol-treated rats compared with sex-matched saline-injected controls. All the effects of propranolol were more prominent in males. The study as a whole disclosed that sexual dimorphism in multiple molecular mechanisms implicated in EAE development may be responsible for greater severity of EAE in male rats and sexually dimorphic action of substances affecting them.
PB  - Springer
T2  - Cellular and Molecular Neurobiology
T1  - β-Adrenoceptor Blockade Moderates Neuroinflammation in Male and Female EAE Rats and Abrogates Sexual Dimorphisms in the Major Neuroinflammatory Pathways by Being More Efficient in Males
DO  - 10.1007/s10571-022-01246-z
ER  - 
@article{
author = "Pilipović, Ivan and Stojić-Vukanić, Zorica and Prijić, Ivana and Jasnić, Nebojša and Đorđević, Jelena and Leposavić, Gordana",
year = "2022",
abstract = "Our previous studies showed more severe experimental autoimmune encephalomyelitis (EAE) in male compared with female adult rats, and moderating effect of propranolol-induced β-adrenoceptor blockade on EAE in females, the effect associated with transcriptional stimulation of Nrf2/HO-1 axis in spinal cord microglia. This study examined putative sexual dimor- phism in propranolol action on EAE severity. Propranolol treatment beginning from the onset of clinical EAE mitigated EAE severity in rats of both sexes, but to a greater extent in males exhibiting higher noradrenaline levels and myeloid cell β 2 -adrenoceptor expression in spinal cord. This correlated with more prominent stimulatory effects of propranolol not only on CX3CL1/CX3CR1/Nrf2/HO-1 cascade, but also on Stat3/Socs3 signaling axis in spinal cord microglia/myeloid cells (mirrored in the decreased Stat3 and the increased Socs3 expression) from male rats compared with their female counterparts. Propranolol diminished the frequency of activated cells among microglia, increased their phagocyting/endocyting capacity, and shifted cytokine secretory profile of microglia/blood-borne myeloid cells towards an anti-inflammatory/neuroprotective phenotype. Additionally, it downregulated the expression of chemokines (CCL2, CCL19/21) driving T-cell/monocyte traf- ficking into spinal cord. Consequently, in propranolol-treated rats fewer activated CD4+ T cells and IL-17+ T cells, including CD4+IL17+ cells coexpressing IFN-γ/GM-CSF, were recovered from spinal cord of propranolol-treated rats compared with sex-matched saline-injected controls. All the effects of propranolol were more prominent in males. The study as a whole disclosed that sexual dimorphism in multiple molecular mechanisms implicated in EAE development may be responsible for greater severity of EAE in male rats and sexually dimorphic action of substances affecting them.",
publisher = "Springer",
journal = "Cellular and Molecular Neurobiology",
title = "β-Adrenoceptor Blockade Moderates Neuroinflammation in Male and Female EAE Rats and Abrogates Sexual Dimorphisms in the Major Neuroinflammatory Pathways by Being More Efficient in Males",
doi = "10.1007/s10571-022-01246-z"
}
Pilipović, I., Stojić-Vukanić, Z., Prijić, I., Jasnić, N., Đorđević, J.,& Leposavić, G.. (2022). β-Adrenoceptor Blockade Moderates Neuroinflammation in Male and Female EAE Rats and Abrogates Sexual Dimorphisms in the Major Neuroinflammatory Pathways by Being More Efficient in Males. in Cellular and Molecular Neurobiology
Springer..
https://doi.org/10.1007/s10571-022-01246-z
Pilipović I, Stojić-Vukanić Z, Prijić I, Jasnić N, Đorđević J, Leposavić G. β-Adrenoceptor Blockade Moderates Neuroinflammation in Male and Female EAE Rats and Abrogates Sexual Dimorphisms in the Major Neuroinflammatory Pathways by Being More Efficient in Males. in Cellular and Molecular Neurobiology. 2022;.
doi:10.1007/s10571-022-01246-z .
Pilipović, Ivan, Stojić-Vukanić, Zorica, Prijić, Ivana, Jasnić, Nebojša, Đorđević, Jelena, Leposavić, Gordana, "β-Adrenoceptor Blockade Moderates Neuroinflammation in Male and Female EAE Rats and Abrogates Sexual Dimorphisms in the Major Neuroinflammatory Pathways by Being More Efficient in Males" in Cellular and Molecular Neurobiology (2022),
https://doi.org/10.1007/s10571-022-01246-z . .

Predictors of Vancomycin-Resistant Enterococcus spp. Intestinal Carriage among High-Risk Patients in University Hospitals in Serbia

Janjušević, Ana; Ćirković, Ivana; Minić, Rajna; Stevanović, Goran; Soldatović, Ivan; Mihaljević, Biljana; Vidović, Ana; Marković-Denić, Ljiljana

(Multidisciplinary Digital Publishing Institute (MDPI), 2022)

TY  - JOUR
AU  - Janjušević, Ana
AU  - Ćirković, Ivana
AU  - Minić, Rajna
AU  - Stevanović, Goran
AU  - Soldatović, Ivan
AU  - Mihaljević, Biljana
AU  - Vidović, Ana
AU  - Marković-Denić, Ljiljana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1260
UR  - http://intor.torlakinstitut.com/handle/123456789/625
AB  - The predictors of intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high-risk patients in the counties of the Southeast Europe Region are insufficiently investigated, yet they could be of key importance in infection control. The aim of the study was to identify risk factors associated with fecal VRE colonization among high-risk inpatients in university hospitals in Serbia. The study comprised 268 inpatients from three university hospitals. Data on patient demographics and clinical characteristics, length of hospital stay, therapy, and procedures were obtained from medical records. Chi-squared tests and univariate and multivariate logistic regressions were performed. Compared to the hemodialysis departments, stay in the geriatric departments, ICUs, and haemato-oncology departments increased the risk for VRE colonization 7.6, 5.4, and 5.5 times, respectively. Compared to inpatients who were hospitalized 48 h before stool sampling for VRE isolation, inpatients hospitalized 3–7, 8–15, and longer than 16 days before sampling had 5.0-, 4.7-, and 6.6-fold higher risk for VRE colonization, respectively. The use of cephalosporins and fluoroquinolones increased the risk for VRE colonization by 2.2 and 1.9 times, respectively. The age ≥ 65 years increased the risk for VRE colonization 2.3 times. In comparison to the University Clinical Centre of Serbia, the hospital stays at Zemun and Zvezdara University Medical Centres were identified as a protector factors. The obtained results could be valuable in predicting the fecal VRE colonization status at patient admission and consequent implementation of infection control measures targeting at-risk inpatients where VRE screening is not routinely performed.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Antibiotics
T1  - Predictors of Vancomycin-Resistant Enterococcus spp. Intestinal Carriage among High-Risk Patients in University Hospitals in Serbia
IS  - 9
SP  - 1228
VL  - 11
DO  - 10.3390/antibiotics11091228
ER  - 
@article{
author = "Janjušević, Ana and Ćirković, Ivana and Minić, Rajna and Stevanović, Goran and Soldatović, Ivan and Mihaljević, Biljana and Vidović, Ana and Marković-Denić, Ljiljana",
year = "2022",
abstract = "The predictors of intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high-risk patients in the counties of the Southeast Europe Region are insufficiently investigated, yet they could be of key importance in infection control. The aim of the study was to identify risk factors associated with fecal VRE colonization among high-risk inpatients in university hospitals in Serbia. The study comprised 268 inpatients from three university hospitals. Data on patient demographics and clinical characteristics, length of hospital stay, therapy, and procedures were obtained from medical records. Chi-squared tests and univariate and multivariate logistic regressions were performed. Compared to the hemodialysis departments, stay in the geriatric departments, ICUs, and haemato-oncology departments increased the risk for VRE colonization 7.6, 5.4, and 5.5 times, respectively. Compared to inpatients who were hospitalized 48 h before stool sampling for VRE isolation, inpatients hospitalized 3–7, 8–15, and longer than 16 days before sampling had 5.0-, 4.7-, and 6.6-fold higher risk for VRE colonization, respectively. The use of cephalosporins and fluoroquinolones increased the risk for VRE colonization by 2.2 and 1.9 times, respectively. The age ≥ 65 years increased the risk for VRE colonization 2.3 times. In comparison to the University Clinical Centre of Serbia, the hospital stays at Zemun and Zvezdara University Medical Centres were identified as a protector factors. The obtained results could be valuable in predicting the fecal VRE colonization status at patient admission and consequent implementation of infection control measures targeting at-risk inpatients where VRE screening is not routinely performed.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Antibiotics",
title = "Predictors of Vancomycin-Resistant Enterococcus spp. Intestinal Carriage among High-Risk Patients in University Hospitals in Serbia",
number = "9",
pages = "1228",
volume = "11",
doi = "10.3390/antibiotics11091228"
}
Janjušević, A., Ćirković, I., Minić, R., Stevanović, G., Soldatović, I., Mihaljević, B., Vidović, A.,& Marković-Denić, L.. (2022). Predictors of Vancomycin-Resistant Enterococcus spp. Intestinal Carriage among High-Risk Patients in University Hospitals in Serbia. in Antibiotics
Multidisciplinary Digital Publishing Institute (MDPI)., 11(9), 1228.
https://doi.org/10.3390/antibiotics11091228
Janjušević A, Ćirković I, Minić R, Stevanović G, Soldatović I, Mihaljević B, Vidović A, Marković-Denić L. Predictors of Vancomycin-Resistant Enterococcus spp. Intestinal Carriage among High-Risk Patients in University Hospitals in Serbia. in Antibiotics. 2022;11(9):1228.
doi:10.3390/antibiotics11091228 .
Janjušević, Ana, Ćirković, Ivana, Minić, Rajna, Stevanović, Goran, Soldatović, Ivan, Mihaljević, Biljana, Vidović, Ana, Marković-Denić, Ljiljana, "Predictors of Vancomycin-Resistant Enterococcus spp. Intestinal Carriage among High-Risk Patients in University Hospitals in Serbia" in Antibiotics, 11, no. 9 (2022):1228,
https://doi.org/10.3390/antibiotics11091228 . .

West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera

Vasić, Ana; Răileanu, Cristian; Körsten, Christin; Vojinović, Dragica; Manić, Marija; Urošević, Aleksandar; Nikolić, Nataša; Dulović, Olga; Tews, Birke Andrea; Petrović, Tamaš; Silaghi, Cornelia; Valčić, Miroslav; Gligić, Ana

(2022)

TY  - JOUR
AU  - Vasić, Ana
AU  - Răileanu, Cristian
AU  - Körsten, Christin
AU  - Vojinović, Dragica
AU  - Manić, Marija
AU  - Urošević, Aleksandar
AU  - Nikolić, Nataša
AU  - Dulović, Olga
AU  - Tews, Birke Andrea
AU  - Petrović, Tamaš
AU  - Silaghi, Cornelia
AU  - Valčić, Miroslav
AU  - Gligić, Ana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1238
UR  - http://intor.torlakinstitut.com/handle/123456789/623
AB  - West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.
T2  - Transboundary and Emerging Diseases
T1  - West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera
EP  - e2515
IS  - 5
SP  - e2506
VL  - 69
DO  - 10.1111/tbed.14593
ER  - 
@article{
author = "Vasić, Ana and Răileanu, Cristian and Körsten, Christin and Vojinović, Dragica and Manić, Marija and Urošević, Aleksandar and Nikolić, Nataša and Dulović, Olga and Tews, Birke Andrea and Petrović, Tamaš and Silaghi, Cornelia and Valčić, Miroslav and Gligić, Ana",
year = "2022",
abstract = "West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.",
journal = "Transboundary and Emerging Diseases",
title = "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera",
pages = "e2515-e2506",
number = "5",
volume = "69",
doi = "10.1111/tbed.14593"
}
Vasić, A., Răileanu, C., Körsten, C., Vojinović, D., Manić, M., Urošević, A., Nikolić, N., Dulović, O., Tews, B. A., Petrović, T., Silaghi, C., Valčić, M.,& Gligić, A.. (2022). West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases, 69(5), e2506-e2515.
https://doi.org/10.1111/tbed.14593
Vasić A, Răileanu C, Körsten C, Vojinović D, Manić M, Urošević A, Nikolić N, Dulović O, Tews BA, Petrović T, Silaghi C, Valčić M, Gligić A. West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases. 2022;69(5):e2506-e2515.
doi:10.1111/tbed.14593 .
Vasić, Ana, Răileanu, Cristian, Körsten, Christin, Vojinović, Dragica, Manić, Marija, Urošević, Aleksandar, Nikolić, Nataša, Dulović, Olga, Tews, Birke Andrea, Petrović, Tamaš, Silaghi, Cornelia, Valčić, Miroslav, Gligić, Ana, "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera" in Transboundary and Emerging Diseases, 69, no. 5 (2022):e2506-e2515,
https://doi.org/10.1111/tbed.14593 . .

B-cell response to seasonal influenza vaccine in mice is amenable to pharmacological modulation through β-adrenoceptor

Bufan, Biljana; Arsenović-Ranin, Nevena; Živković, Irena; Petrović, Raisa; Leposavić, Gordana

(Elsevier, 2022)

TY  - JOUR
AU  - Bufan, Biljana
AU  - Arsenović-Ranin, Nevena
AU  - Živković, Irena
AU  - Petrović, Raisa
AU  - Leposavić, Gordana
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/620
AB  - Aims: Given that deprivation of noradrenaline acting on lymphocytes through β-adrenoceptor influences antibody response, the effects of propranolol treatment beginning two days before immunization with quadrivalent inactivated influenza vaccine (QIV) on IgG response and underlying cellular molecular mechanism in mice were investigated.

Main methods: Twenty-one days post-immunization the total QIV antigen-specific IgG titer and IgG subclass titers in sera were determined using ELISA. Additionally, the total counts of germinal centre (GC) B cells, T follicular helper (Tfh) and T follicular regulatory (Tfr) cells in draining lymph nodes (dLNs) and spleens, in vitro proliferation of interacting B cells and Th cells and IL-21 synthesis in Th cells in response to QIV antigens and/or mitogen were attested using flow cytometry analysis. In QIV antigen-stimulated dLN cell and splenocyte cultures were also measured concentrations of INF-γ and IL-4, cytokines upregulating IgG2a and IgG1 synthesis, respectively.

Key findings: Propranolol decreased the total QIV antigen-specific IgG titer. This correlated with lower GC B cell count and the shift in Tfr/Tfh cell and Tfr/GC B cell ratio towards Tfr in propranolol-treated mice compared with controls. Consistently, QIV antigen-stimulated proliferation of B cells and Th cells from propranolol-treated mice in vitro was impaired. This correlated with the lower frequency of QIV antigen-specific IL-21-producing cells among Th cells. Additionally, in propranolol-treated mice, in accordance with the changes in INF-γ/IL-4 ratio in dLN cell/splenocyte cultures, serum IgG2a/IgG1 ratio was shifted towards IgG1 reflecting decreased IgG2a response.

Significance: The study indicates that chronic propranolol treatment may impair response to QIV.
PB  - Elsevier
T2  - Life Sciences
T1  - B-cell response to seasonal influenza vaccine in mice is amenable to pharmacological modulation through β-adrenoceptor
SP  - 120617
VL  - 301
DO  - 10.1016/j.lfs.2022.120617
ER  - 
@article{
author = "Bufan, Biljana and Arsenović-Ranin, Nevena and Živković, Irena and Petrović, Raisa and Leposavić, Gordana",
year = "2022",
abstract = "Aims: Given that deprivation of noradrenaline acting on lymphocytes through β-adrenoceptor influences antibody response, the effects of propranolol treatment beginning two days before immunization with quadrivalent inactivated influenza vaccine (QIV) on IgG response and underlying cellular molecular mechanism in mice were investigated.

Main methods: Twenty-one days post-immunization the total QIV antigen-specific IgG titer and IgG subclass titers in sera were determined using ELISA. Additionally, the total counts of germinal centre (GC) B cells, T follicular helper (Tfh) and T follicular regulatory (Tfr) cells in draining lymph nodes (dLNs) and spleens, in vitro proliferation of interacting B cells and Th cells and IL-21 synthesis in Th cells in response to QIV antigens and/or mitogen were attested using flow cytometry analysis. In QIV antigen-stimulated dLN cell and splenocyte cultures were also measured concentrations of INF-γ and IL-4, cytokines upregulating IgG2a and IgG1 synthesis, respectively.

Key findings: Propranolol decreased the total QIV antigen-specific IgG titer. This correlated with lower GC B cell count and the shift in Tfr/Tfh cell and Tfr/GC B cell ratio towards Tfr in propranolol-treated mice compared with controls. Consistently, QIV antigen-stimulated proliferation of B cells and Th cells from propranolol-treated mice in vitro was impaired. This correlated with the lower frequency of QIV antigen-specific IL-21-producing cells among Th cells. Additionally, in propranolol-treated mice, in accordance with the changes in INF-γ/IL-4 ratio in dLN cell/splenocyte cultures, serum IgG2a/IgG1 ratio was shifted towards IgG1 reflecting decreased IgG2a response.

Significance: The study indicates that chronic propranolol treatment may impair response to QIV.",
publisher = "Elsevier",
journal = "Life Sciences",
title = "B-cell response to seasonal influenza vaccine in mice is amenable to pharmacological modulation through β-adrenoceptor",
pages = "120617",
volume = "301",
doi = "10.1016/j.lfs.2022.120617"
}
Bufan, B., Arsenović-Ranin, N., Živković, I., Petrović, R.,& Leposavić, G.. (2022). B-cell response to seasonal influenza vaccine in mice is amenable to pharmacological modulation through β-adrenoceptor. in Life Sciences
Elsevier., 301, 120617.
https://doi.org/10.1016/j.lfs.2022.120617
Bufan B, Arsenović-Ranin N, Živković I, Petrović R, Leposavić G. B-cell response to seasonal influenza vaccine in mice is amenable to pharmacological modulation through β-adrenoceptor. in Life Sciences. 2022;301:120617.
doi:10.1016/j.lfs.2022.120617 .
Bufan, Biljana, Arsenović-Ranin, Nevena, Živković, Irena, Petrović, Raisa, Leposavić, Gordana, "B-cell response to seasonal influenza vaccine in mice is amenable to pharmacological modulation through β-adrenoceptor" in Life Sciences, 301 (2022):120617,
https://doi.org/10.1016/j.lfs.2022.120617 . .
2

Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans

Dragačević, Luka; Lopandić, Zorana; Gavrović-Jankulović, Marija; Živković, Irena; Blagojević, Veljko; Polović, Natalija; Minić, Rajna

(Springer, 2022)

TY  - JOUR
AU  - Dragačević, Luka
AU  - Lopandić, Zorana
AU  - Gavrović-Jankulović, Marija
AU  - Živković, Irena
AU  - Blagojević, Veljko
AU  - Polović, Natalija
AU  - Minić, Rajna
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/621
AB  - The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T1  - Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans
EP  - 2060
SP  - 2047
VL  - 194
DO  - 10.1007/s12010-021-03772-w
ER  - 
@article{
author = "Dragačević, Luka and Lopandić, Zorana and Gavrović-Jankulović, Marija and Živković, Irena and Blagojević, Veljko and Polović, Natalija and Minić, Rajna",
year = "2022",
abstract = "The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology",
title = "Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans",
pages = "2060-2047",
volume = "194",
doi = "10.1007/s12010-021-03772-w"
}
Dragačević, L., Lopandić, Z., Gavrović-Jankulović, M., Živković, I., Blagojević, V., Polović, N.,& Minić, R.. (2022). Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. in Applied Biochemistry and Biotechnology
Springer., 194, 2047-2060.
https://doi.org/10.1007/s12010-021-03772-w
Dragačević L, Lopandić Z, Gavrović-Jankulović M, Živković I, Blagojević V, Polović N, Minić R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. in Applied Biochemistry and Biotechnology. 2022;194:2047-2060.
doi:10.1007/s12010-021-03772-w .
Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, Minić, Rajna, "Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans" in Applied Biochemistry and Biotechnology, 194 (2022):2047-2060,
https://doi.org/10.1007/s12010-021-03772-w . .
1
1

Retrospective Analysis of Six Years of Acute Flaccid Paralysis Surveillance and Polio Vaccine Coverage Reported by Italy, Serbia, Bosnia and Herzegovina, Montenegro, Bulgaria, Kosovo, Albania, North Macedonia, Malta, and Greece

Fontana, Stefano; Buttinelli, Gabriele; Fiore, Stefano; Amato, Concetta; Pataracchia, Marco; Kota, Majlinda; Aćimović, Jela; Blažević, Mia; Mulaomerović, Mirsada; Nikolaeva-Glomb, Lubomira; Mentis, Andreas; Voulgari-Kokota, Androniki; Gashi, Luljeta; Kaçaniku-Gunga, Pranvera; Barbara, Christopher; Melillo, Jackie; Protic, Jelena; Filipović-Vignjević, Svetlana; O’Connor, Patrick M.; D’Alberto, Alessandra; Orioli, Riccardo; Siddu, Andrea; Saxentoff, Eugene; Stefanelli, Paola

(MDPI, 2022)

TY  - JOUR
AU  - Fontana, Stefano
AU  - Buttinelli, Gabriele
AU  - Fiore, Stefano
AU  - Amato, Concetta
AU  - Pataracchia, Marco
AU  - Kota, Majlinda
AU  - Aćimović, Jela
AU  - Blažević, Mia
AU  - Mulaomerović, Mirsada
AU  - Nikolaeva-Glomb, Lubomira
AU  - Mentis, Andreas
AU  - Voulgari-Kokota, Androniki
AU  - Gashi, Luljeta
AU  - Kaçaniku-Gunga, Pranvera
AU  - Barbara, Christopher
AU  - Melillo, Jackie
AU  - Protic, Jelena
AU  - Filipović-Vignjević, Svetlana
AU  - O’Connor, Patrick M.
AU  - D’Alberto, Alessandra
AU  - Orioli, Riccardo
AU  - Siddu, Andrea
AU  - Saxentoff, Eugene
AU  - Stefanelli, Paola
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/618
AB  - Here we analyzed six years of acute flaccid paralysis (AFP) surveillance, from 2015 to 2020, of 10 countries linked to the WHO Regional Reference Laboratory, at the Istituto Superiore di Sanità, Italy. The analysis also comprises the polio vaccine coverage available (2015–2019) and enterovirus (EV) identification and typing data. Centralized Information System for Infectious Diseases and Laboratory Data Management System databases were used to obtain data on AFP indicators and laboratory performance and countries’ vaccine coverage from 2015 to 2019. EV isolation, identification, and typing were performed by each country according to WHO protocols. Overall, a general AFP underreporting was observed. Non-Polio Enterovirus (NPEV) typing showed a high heterogeneity: over the years, several genotypes of coxsackievirus and echovirus have been identified. The polio vaccine coverage, for the data available, differs among countries. This evaluation allows for the collection, for the first time, of data from the countries of the Balkan area regarding AFP surveillance and polio vaccine coverage. The need, for some countries, to enhance the surveillance systems and to promote the polio vaccine uptake, in order to maintain the polio-free status, is evident.
PB  - MDPI
T2  - Vaccines
T1  - Retrospective Analysis of Six Years of Acute Flaccid Paralysis Surveillance and Polio Vaccine Coverage Reported by Italy, Serbia, Bosnia and Herzegovina, Montenegro, Bulgaria, Kosovo, Albania, North Macedonia, Malta, and Greece
IS  - 1
SP  - 44
VL  - 10
DO  - 10.3390/vaccines10010044
ER  - 
@article{
author = "Fontana, Stefano and Buttinelli, Gabriele and Fiore, Stefano and Amato, Concetta and Pataracchia, Marco and Kota, Majlinda and Aćimović, Jela and Blažević, Mia and Mulaomerović, Mirsada and Nikolaeva-Glomb, Lubomira and Mentis, Andreas and Voulgari-Kokota, Androniki and Gashi, Luljeta and Kaçaniku-Gunga, Pranvera and Barbara, Christopher and Melillo, Jackie and Protic, Jelena and Filipović-Vignjević, Svetlana and O’Connor, Patrick M. and D’Alberto, Alessandra and Orioli, Riccardo and Siddu, Andrea and Saxentoff, Eugene and Stefanelli, Paola",
year = "2022",
abstract = "Here we analyzed six years of acute flaccid paralysis (AFP) surveillance, from 2015 to 2020, of 10 countries linked to the WHO Regional Reference Laboratory, at the Istituto Superiore di Sanità, Italy. The analysis also comprises the polio vaccine coverage available (2015–2019) and enterovirus (EV) identification and typing data. Centralized Information System for Infectious Diseases and Laboratory Data Management System databases were used to obtain data on AFP indicators and laboratory performance and countries’ vaccine coverage from 2015 to 2019. EV isolation, identification, and typing were performed by each country according to WHO protocols. Overall, a general AFP underreporting was observed. Non-Polio Enterovirus (NPEV) typing showed a high heterogeneity: over the years, several genotypes of coxsackievirus and echovirus have been identified. The polio vaccine coverage, for the data available, differs among countries. This evaluation allows for the collection, for the first time, of data from the countries of the Balkan area regarding AFP surveillance and polio vaccine coverage. The need, for some countries, to enhance the surveillance systems and to promote the polio vaccine uptake, in order to maintain the polio-free status, is evident.",
publisher = "MDPI",
journal = "Vaccines",
title = "Retrospective Analysis of Six Years of Acute Flaccid Paralysis Surveillance and Polio Vaccine Coverage Reported by Italy, Serbia, Bosnia and Herzegovina, Montenegro, Bulgaria, Kosovo, Albania, North Macedonia, Malta, and Greece",
number = "1",
pages = "44",
volume = "10",
doi = "10.3390/vaccines10010044"
}
Fontana, S., Buttinelli, G., Fiore, S., Amato, C., Pataracchia, M., Kota, M., Aćimović, J., Blažević, M., Mulaomerović, M., Nikolaeva-Glomb, L., Mentis, A., Voulgari-Kokota, A., Gashi, L., Kaçaniku-Gunga, P., Barbara, C., Melillo, J., Protic, J., Filipović-Vignjević, S., O’Connor, P. M., D’Alberto, A., Orioli, R., Siddu, A., Saxentoff, E.,& Stefanelli, P.. (2022). Retrospective Analysis of Six Years of Acute Flaccid Paralysis Surveillance and Polio Vaccine Coverage Reported by Italy, Serbia, Bosnia and Herzegovina, Montenegro, Bulgaria, Kosovo, Albania, North Macedonia, Malta, and Greece. in Vaccines
MDPI., 10(1), 44.
https://doi.org/10.3390/vaccines10010044
Fontana S, Buttinelli G, Fiore S, Amato C, Pataracchia M, Kota M, Aćimović J, Blažević M, Mulaomerović M, Nikolaeva-Glomb L, Mentis A, Voulgari-Kokota A, Gashi L, Kaçaniku-Gunga P, Barbara C, Melillo J, Protic J, Filipović-Vignjević S, O’Connor PM, D’Alberto A, Orioli R, Siddu A, Saxentoff E, Stefanelli P. Retrospective Analysis of Six Years of Acute Flaccid Paralysis Surveillance and Polio Vaccine Coverage Reported by Italy, Serbia, Bosnia and Herzegovina, Montenegro, Bulgaria, Kosovo, Albania, North Macedonia, Malta, and Greece. in Vaccines. 2022;10(1):44.
doi:10.3390/vaccines10010044 .
Fontana, Stefano, Buttinelli, Gabriele, Fiore, Stefano, Amato, Concetta, Pataracchia, Marco, Kota, Majlinda, Aćimović, Jela, Blažević, Mia, Mulaomerović, Mirsada, Nikolaeva-Glomb, Lubomira, Mentis, Andreas, Voulgari-Kokota, Androniki, Gashi, Luljeta, Kaçaniku-Gunga, Pranvera, Barbara, Christopher, Melillo, Jackie, Protic, Jelena, Filipović-Vignjević, Svetlana, O’Connor, Patrick M., D’Alberto, Alessandra, Orioli, Riccardo, Siddu, Andrea, Saxentoff, Eugene, Stefanelli, Paola, "Retrospective Analysis of Six Years of Acute Flaccid Paralysis Surveillance and Polio Vaccine Coverage Reported by Italy, Serbia, Bosnia and Herzegovina, Montenegro, Bulgaria, Kosovo, Albania, North Macedonia, Malta, and Greece" in Vaccines, 10, no. 1 (2022):44,
https://doi.org/10.3390/vaccines10010044 . .
1
1
1

Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers

Popović, Mina; Stojanović, Marijana; Veličković, Zlate; Kovačević, Ana; Miljković, Radmila; Mirković, Nemanja; Marinković, Aleksandar D.

(Elsevier, 2021)

TY  - JOUR
AU  - Popović, Mina
AU  - Stojanović, Marijana
AU  - Veličković, Zlate
AU  - Kovačević, Ana
AU  - Miljković, Radmila
AU  - Mirković, Nemanja
AU  - Marinković, Aleksandar D.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4594
UR  - http://intor.torlakinstitut.com/handle/123456789/626
AB  - In this study, Lactobacillus reuteri B2was isolated fromthe feces of C57BL/6 mice and assessed on probiotic activity.L. reuteri B2was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0was64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2showed maximumdiameters against Klebsiela oxytoca J7 (12.5±0.71mm).Wefurther hypothesized if L. reuteriB2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of theappropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulatedinto sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterizationmaterials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy(SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at lowpH from2.0 to 4.0and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobialactivity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices(SGJ).
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers
EP  - 434
SP  - 423
VL  - 183
DO  - 10.1016/j.ijbiomac.2021.04.177
ER  - 
@article{
author = "Popović, Mina and Stojanović, Marijana and Veličković, Zlate and Kovačević, Ana and Miljković, Radmila and Mirković, Nemanja and Marinković, Aleksandar D.",
year = "2021",
abstract = "In this study, Lactobacillus reuteri B2was isolated fromthe feces of C57BL/6 mice and assessed on probiotic activity.L. reuteri B2was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0was64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2showed maximumdiameters against Klebsiela oxytoca J7 (12.5±0.71mm).Wefurther hypothesized if L. reuteriB2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of theappropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulatedinto sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterizationmaterials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy(SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at lowpH from2.0 to 4.0and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobialactivity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices(SGJ).",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers",
pages = "434-423",
volume = "183",
doi = "10.1016/j.ijbiomac.2021.04.177"
}
Popović, M., Stojanović, M., Veličković, Z., Kovačević, A., Miljković, R., Mirković, N.,& Marinković, A. D.. (2021). Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. in International Journal of Biological Macromolecules
Elsevier., 183, 423-434.
https://doi.org/10.1016/j.ijbiomac.2021.04.177
Popović M, Stojanović M, Veličković Z, Kovačević A, Miljković R, Mirković N, Marinković AD. Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. in International Journal of Biological Macromolecules. 2021;183:423-434.
doi:10.1016/j.ijbiomac.2021.04.177 .
Popović, Mina, Stojanović, Marijana, Veličković, Zlate, Kovačević, Ana, Miljković, Radmila, Mirković, Nemanja, Marinković, Aleksandar D., "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers" in International Journal of Biological Macromolecules, 183 (2021):423-434,
https://doi.org/10.1016/j.ijbiomac.2021.04.177 . .
6
2
6

Supplementary material for: "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers"

Popović, Mina; Stojanović, Marijana; Veličković, Zlate; Kovačević, Ana; Miljković, Radmila; Mirković, Nemanja; Marinković, Aleksandar D.

(Elsevier, 2021)

TY  - DATA
AU  - Popović, Mina
AU  - Stojanović, Marijana
AU  - Veličković, Zlate
AU  - Kovačević, Ana
AU  - Miljković, Radmila
AU  - Mirković, Nemanja
AU  - Marinković, Aleksandar D.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4596
UR  - http://intor.torlakinstitut.com/handle/123456789/627
AB  - Preparation of materials for encapsulation (Materials, Laboratory isolation of ricinoleic acid, Laboratory preparation of starch maleate monoester, Characterization, Statistical analysis). Additional results (Antimicrobial activity, Optimization of encapsulation yield, Size distribution of alginate beads in acidic conditions). additional references
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Supplementary material for: "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers"
DO  - 10.1016/j.ijbiomac.2021.04.177
ER  - 
@misc{
author = "Popović, Mina and Stojanović, Marijana and Veličković, Zlate and Kovačević, Ana and Miljković, Radmila and Mirković, Nemanja and Marinković, Aleksandar D.",
year = "2021",
abstract = "Preparation of materials for encapsulation (Materials, Laboratory isolation of ricinoleic acid, Laboratory preparation of starch maleate monoester, Characterization, Statistical analysis). Additional results (Antimicrobial activity, Optimization of encapsulation yield, Size distribution of alginate beads in acidic conditions). additional references",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Supplementary material for: "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers"",
doi = "10.1016/j.ijbiomac.2021.04.177"
}
Popović, M., Stojanović, M., Veličković, Z., Kovačević, A., Miljković, R., Mirković, N.,& Marinković, A. D.. (2021). Supplementary material for: "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers". in International Journal of Biological Macromolecules
Elsevier..
https://doi.org/10.1016/j.ijbiomac.2021.04.177
Popović M, Stojanović M, Veličković Z, Kovačević A, Miljković R, Mirković N, Marinković AD. Supplementary material for: "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers". in International Journal of Biological Macromolecules. 2021;.
doi:10.1016/j.ijbiomac.2021.04.177 .
Popović, Mina, Stojanović, Marijana, Veličković, Zlate, Kovačević, Ana, Miljković, Radmila, Mirković, Nemanja, Marinković, Aleksandar D., "Supplementary material for: "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers"" in International Journal of Biological Macromolecules (2021),
https://doi.org/10.1016/j.ijbiomac.2021.04.177 . .
6
2

Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers

Popović, Mina; Stojanović, Marijana; Veličković, Zlate; Kovačević, Ana; Miljković, Radmila; Mirković, Nemanja; Marinković, Aleksandar D.

(Elsevier, 2021)

TY  - JOUR
AU  - Popović, Mina
AU  - Stojanović, Marijana
AU  - Veličković, Zlate
AU  - Kovačević, Ana
AU  - Miljković, Radmila
AU  - Mirković, Nemanja
AU  - Marinković, Aleksandar D.
PY  - 2021
UR  - https://cer.ihtm.bg.ac.rs/handle/123456789/4595
UR  - http://intor.torlakinstitut.com/handle/123456789/628
AB  - In this study, Lactobacillus reuteri B2was isolated fromthe feces of C57BL/6 mice and assessed on probiotic activity.L. reuteri B2was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0was64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2showed maximumdiameters against Klebsiela oxytoca J7 (12.5±0.71mm).Wefurther hypothesized if L. reuteriB2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of theappropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulatedinto sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterizationmaterials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy(SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at lowpH from2.0 to 4.0and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobialactivity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices(SGJ).
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers
EP  - 434
SP  - 423
VL  - 183
DO  - 10.1016/j.ijbiomac.2021.04.177
ER  - 
@article{
author = "Popović, Mina and Stojanović, Marijana and Veličković, Zlate and Kovačević, Ana and Miljković, Radmila and Mirković, Nemanja and Marinković, Aleksandar D.",
year = "2021",
abstract = "In this study, Lactobacillus reuteri B2was isolated fromthe feces of C57BL/6 mice and assessed on probiotic activity.L. reuteri B2was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0was64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2showed maximumdiameters against Klebsiela oxytoca J7 (12.5±0.71mm).Wefurther hypothesized if L. reuteriB2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of theappropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulatedinto sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterizationmaterials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy(SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at lowpH from2.0 to 4.0and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobialactivity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices(SGJ).",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers",
pages = "434-423",
volume = "183",
doi = "10.1016/j.ijbiomac.2021.04.177"
}
Popović, M., Stojanović, M., Veličković, Z., Kovačević, A., Miljković, R., Mirković, N.,& Marinković, A. D.. (2021). Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. in International Journal of Biological Macromolecules
Elsevier., 183, 423-434.
https://doi.org/10.1016/j.ijbiomac.2021.04.177
Popović M, Stojanović M, Veličković Z, Kovačević A, Miljković R, Mirković N, Marinković AD. Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. in International Journal of Biological Macromolecules. 2021;183:423-434.
doi:10.1016/j.ijbiomac.2021.04.177 .
Popović, Mina, Stojanović, Marijana, Veličković, Zlate, Kovačević, Ana, Miljković, Radmila, Mirković, Nemanja, Marinković, Aleksandar D., "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers" in International Journal of Biological Macromolecules, 183 (2021):423-434,
https://doi.org/10.1016/j.ijbiomac.2021.04.177 . .
6
2
6

Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases

Stojić-Vukanić, Zorica; Pilipović, Ivan; Arsenović-Ranin, Nevena; Dimitrijević, Mirjana; Leposavić, Gordana

(Elsevier B.V., 2021)

TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Pilipović, Ivan
AU  - Arsenović-Ranin, Nevena
AU  - Dimitrijević, Mirjana
AU  - Leposavić, Gordana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3946
UR  - http://intor.torlakinstitut.com/handle/123456789/624
AB  - The incidence of multiple sclerosis (MS) and susceptibility of animals to experimental autoimmune encephalomyelitis (EAE), the most commonly used experimental model of MS, decrease with aging. Generally, autoimmune diseases develop as the ultimate outcome of an imbalance between damaging immune responses against self and regulatory immune responses (keeping the former under control). Thus, in this review the age-related changes possibly underlying this balance were discussed. Specifically, considering the central role of T cells in MS/EAE, the impact of aging on overall functional capacity (reflecting both overall count and individual functional cell properties) of self-reactive conventional T cells (Tcons) and FoxP3+ regulatory T cells (Tregs), as the most potent immunoregulatory/suppressive cells, was analyzed, as well. The analysis encompasses three distinct compartments: thymus (the primary lymphoid organ responsible for the elimination of self-reactive T cells – negative selection and the generation of Tregs, compensating for imperfections of the negative selection), peripheral blood/lymphoid tissues (“afferent” compartment), and brain/spinal cord tissues (“target” compartment). Given that the incidence of MS and susceptibility of animals to EAE are greater in women/females than in age-matched men/males, sex as independent variable was also considered. In conclusion, with aging, sex-specific alterations in the balance of self-reactive Tcons/Tregs are likely to occur not only in the thymus/”afferent” compartment, but also in the “target” compartment, reflecting multifaceted changes in both T-cell types. Their in depth understanding is important not only for envisaging effects of aging, but also for designing interventions to slow-down aging without any adverse effect on incidence of autoimmune diseases.
PB  - Elsevier B.V.
T2  - Immunology Letters
T1  - Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases
EP  - 59
SP  - 42
VL  - 239
DO  - 10.1016/j.imlet.2021.08.003
ER  - 
@article{
author = "Stojić-Vukanić, Zorica and Pilipović, Ivan and Arsenović-Ranin, Nevena and Dimitrijević, Mirjana and Leposavić, Gordana",
year = "2021",
abstract = "The incidence of multiple sclerosis (MS) and susceptibility of animals to experimental autoimmune encephalomyelitis (EAE), the most commonly used experimental model of MS, decrease with aging. Generally, autoimmune diseases develop as the ultimate outcome of an imbalance between damaging immune responses against self and regulatory immune responses (keeping the former under control). Thus, in this review the age-related changes possibly underlying this balance were discussed. Specifically, considering the central role of T cells in MS/EAE, the impact of aging on overall functional capacity (reflecting both overall count and individual functional cell properties) of self-reactive conventional T cells (Tcons) and FoxP3+ regulatory T cells (Tregs), as the most potent immunoregulatory/suppressive cells, was analyzed, as well. The analysis encompasses three distinct compartments: thymus (the primary lymphoid organ responsible for the elimination of self-reactive T cells – negative selection and the generation of Tregs, compensating for imperfections of the negative selection), peripheral blood/lymphoid tissues (“afferent” compartment), and brain/spinal cord tissues (“target” compartment). Given that the incidence of MS and susceptibility of animals to EAE are greater in women/females than in age-matched men/males, sex as independent variable was also considered. In conclusion, with aging, sex-specific alterations in the balance of self-reactive Tcons/Tregs are likely to occur not only in the thymus/”afferent” compartment, but also in the “target” compartment, reflecting multifaceted changes in both T-cell types. Their in depth understanding is important not only for envisaging effects of aging, but also for designing interventions to slow-down aging without any adverse effect on incidence of autoimmune diseases.",
publisher = "Elsevier B.V.",
journal = "Immunology Letters",
title = "Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases",
pages = "59-42",
volume = "239",
doi = "10.1016/j.imlet.2021.08.003"
}
Stojić-Vukanić, Z., Pilipović, I., Arsenović-Ranin, N., Dimitrijević, M.,& Leposavić, G.. (2021). Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases. in Immunology Letters
Elsevier B.V.., 239, 42-59.
https://doi.org/10.1016/j.imlet.2021.08.003
Stojić-Vukanić Z, Pilipović I, Arsenović-Ranin N, Dimitrijević M, Leposavić G. Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases. in Immunology Letters. 2021;239:42-59.
doi:10.1016/j.imlet.2021.08.003 .
Stojić-Vukanić, Zorica, Pilipović, Ivan, Arsenović-Ranin, Nevena, Dimitrijević, Mirjana, Leposavić, Gordana, "Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases" in Immunology Letters, 239 (2021):42-59,
https://doi.org/10.1016/j.imlet.2021.08.003 . .

Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs

Frohns, Antonia; Stojanović, Marijana; Barisani-Asenbauer, Talin; Kuratli, Jasmin; Borel, Nicole; Inić-Kanada, Aleksandra

(Elsevier, 2021)

TY  - JOUR
AU  - Frohns, Antonia
AU  - Stojanović, Marijana
AU  - Barisani-Asenbauer, Talin
AU  - Kuratli, Jasmin
AU  - Borel, Nicole
AU  - Inić-Kanada, Aleksandra
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/617
AB  - Water-filtered infrared A and visible light (wIRA/VIS), shown to reduce chlamydial infections in vitro and in vivo, might represent an innovative therapeutic approach against trachoma, a neglected tropical disease caused by ocular infection with the bacterium C. trachomatis. In this in vivo study, we assessed the impact of wIRA radiation in combination with VIS (wavelength range 595–1400 nm, intensity 2100 W/m2) on the retina and cornea in a guinea pig animal model of inclusion conjunctivitis. We investigated the effects 19 days after wIRA/VIS irradiation by comparing a single and double wIRA/VIS treatment with a sham control. By immunolabeling and western blot analyses of critical heat- and stress-responsive proteins, we could not detect wIRA/VIS-induced changes in their expression pattern. Also, immunolabeling of specific retinal marker proteins revealed no changes in their expression pattern caused by the treatment. Our preclinical study suggests wIRA/VIS as a promising and safe therapeutic tool to treat ocular chlamydial infections.
PB  - Elsevier
T2  - Journal of Photochemistry and Photobiology B: Biology
T1  - Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs
SP  - 112306
VL  - 224
DO  - 10.1016/j.jphotobiol.2021.112306
ER  - 
@article{
author = "Frohns, Antonia and Stojanović, Marijana and Barisani-Asenbauer, Talin and Kuratli, Jasmin and Borel, Nicole and Inić-Kanada, Aleksandra",
year = "2021",
abstract = "Water-filtered infrared A and visible light (wIRA/VIS), shown to reduce chlamydial infections in vitro and in vivo, might represent an innovative therapeutic approach against trachoma, a neglected tropical disease caused by ocular infection with the bacterium C. trachomatis. In this in vivo study, we assessed the impact of wIRA radiation in combination with VIS (wavelength range 595–1400 nm, intensity 2100 W/m2) on the retina and cornea in a guinea pig animal model of inclusion conjunctivitis. We investigated the effects 19 days after wIRA/VIS irradiation by comparing a single and double wIRA/VIS treatment with a sham control. By immunolabeling and western blot analyses of critical heat- and stress-responsive proteins, we could not detect wIRA/VIS-induced changes in their expression pattern. Also, immunolabeling of specific retinal marker proteins revealed no changes in their expression pattern caused by the treatment. Our preclinical study suggests wIRA/VIS as a promising and safe therapeutic tool to treat ocular chlamydial infections.",
publisher = "Elsevier",
journal = "Journal of Photochemistry and Photobiology B: Biology",
title = "Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs",
pages = "112306",
volume = "224",
doi = "10.1016/j.jphotobiol.2021.112306"
}
Frohns, A., Stojanović, M., Barisani-Asenbauer, T., Kuratli, J., Borel, N.,& Inić-Kanada, A.. (2021). Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs. in Journal of Photochemistry and Photobiology B: Biology
Elsevier., 224, 112306.
https://doi.org/10.1016/j.jphotobiol.2021.112306
Frohns A, Stojanović M, Barisani-Asenbauer T, Kuratli J, Borel N, Inić-Kanada A. Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs. in Journal of Photochemistry and Photobiology B: Biology. 2021;224:112306.
doi:10.1016/j.jphotobiol.2021.112306 .
Frohns, Antonia, Stojanović, Marijana, Barisani-Asenbauer, Talin, Kuratli, Jasmin, Borel, Nicole, Inić-Kanada, Aleksandra, "Effects of water-filtered infrared A and visible light (wIRA/VIS) radiation on heat- and stress-responsive proteins in the retina and cornea of guinea pigs" in Journal of Photochemistry and Photobiology B: Biology, 224 (2021):112306,
https://doi.org/10.1016/j.jphotobiol.2021.112306 . .
1
1
1

BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms

Lopandic, Zorana; Dragacevic, Luka; Popovic, Dragan; Andjelkovic, Uros; Minic, Rajna; Gavrovic-Jankulovic, Marija

(MDPI, 2021)

TY  - JOUR
AU  - Lopandic, Zorana
AU  - Dragacevic, Luka
AU  - Popovic, Dragan
AU  - Andjelkovic, Uros
AU  - Minic, Rajna
AU  - Gavrovic-Jankulovic, Marija
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/615
AB  - Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi. For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli. BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm). More than 65% of the primary structure was confirmed by mass spectrometry. Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant. BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate. BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms
PB  - MDPI
T2  - Biomolecules
T1  - BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms
IS  - 2
SP  - 180
VL  - 11(2)
VL  - 11
DO  - 10.3390/biom11020180
ER  - 
@article{
author = "Lopandic, Zorana and Dragacevic, Luka and Popovic, Dragan and Andjelkovic, Uros and Minic, Rajna and Gavrovic-Jankulovic, Marija",
year = "2021",
abstract = "Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi. For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli. BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm). More than 65% of the primary structure was confirmed by mass spectrometry. Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant. BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate. BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms",
publisher = "MDPI",
journal = "Biomolecules",
title = "BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms",
number = "2",
pages = "180",
volume = "11(2), 11",
doi = "10.3390/biom11020180"
}
Lopandic, Z., Dragacevic, L., Popovic, D., Andjelkovic, U., Minic, R.,& Gavrovic-Jankulovic, M.. (2021). BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms. in Biomolecules
MDPI., 11(2)(2), 180.
https://doi.org/10.3390/biom11020180
Lopandic Z, Dragacevic L, Popovic D, Andjelkovic U, Minic R, Gavrovic-Jankulovic M. BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms. in Biomolecules. 2021;11(2)(2):180.
doi:10.3390/biom11020180 .
Lopandic, Zorana, Dragacevic, Luka, Popovic, Dragan, Andjelkovic, Uros, Minic, Rajna, Gavrovic-Jankulovic, Marija, "BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms" in Biomolecules, 11(2), no. 2 (2021):180,
https://doi.org/10.3390/biom11020180 . .
4
2
4

Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report

Janjusevic, Ana; Markovic Denic, Ljiljana; Minic, Rajna; Anita, Grgurevic; Cirkovic, Ivana

(BMC, 2021)

TY  - JOUR
AU  - Janjusevic, Ana
AU  - Markovic Denic, Ljiljana
AU  - Minic, Rajna
AU  - Anita, Grgurevic
AU  - Cirkovic, Ivana
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/614
AB  - Background: The screening for intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high risk patients in the Balkan region and molecular epidemiology of VRE is insufficiently investigated, yet it could be of key importance in infection control. The aim of this study was to provide baseline data on VRE intestinal carriage among high-risk patients in Serbian university hospitals, to determine the phenotypic/genotypic profiles of the isolated VRE, to obtain knowledge of local resistance patterns and bridge the gaps in current VRE surveillance.

Methods: The VRE reservoir was investigated using stool samples from 268 inpatients. Characterization of isolated VRE stains consisted of BD Phoenix system, genotypic identification, glycopeptide and quinupristin-dalfopristin (Q-D) resistance probing, virulence gene (esp, hyl, efaA, asa1, gelE, cpd) detection and MLVA. Biofilm formation was evaluated by the microtiter plate method.

Results: VRE carriage prevalence among at-risk patients was 28.7%. All VRE strains were vanA positive multidrug-resistant Enterococcus faecium (VRfm), harboring ermB-1 (38.9%), esp (84%), efaA (71.2%), hyl (54.5%), asa1 (23.4%), gelE and cpd (11.6%) each. Ability of biofilm production was detected in 20.8%. Genetic relatedness of the isolates revealed 13 clusters, heterogeneous picture and 25 unique MTs profiles.

Conclusion: The obtained prevalence of VRE intestinal carriage among high-risk inpatients in Serbia is higher than the European average, with high percentage of multidrug resistance. The emergence of resistance to Q-D is of particular concern. Close monitoring of pattern of resistance and strict adherence to specific guidelines are urgently needed in Serbia.
PB  - BMC
T2  - Annals of Clinical Microbiology and Antimicrobials
T1  - Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report
SP  - 18
VL  - 20
DO  - 10.1186/s12941-021-00423-0
ER  - 
@article{
author = "Janjusevic, Ana and Markovic Denic, Ljiljana and Minic, Rajna and Anita, Grgurevic and Cirkovic, Ivana",
year = "2021",
abstract = "Background: The screening for intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high risk patients in the Balkan region and molecular epidemiology of VRE is insufficiently investigated, yet it could be of key importance in infection control. The aim of this study was to provide baseline data on VRE intestinal carriage among high-risk patients in Serbian university hospitals, to determine the phenotypic/genotypic profiles of the isolated VRE, to obtain knowledge of local resistance patterns and bridge the gaps in current VRE surveillance.

Methods: The VRE reservoir was investigated using stool samples from 268 inpatients. Characterization of isolated VRE stains consisted of BD Phoenix system, genotypic identification, glycopeptide and quinupristin-dalfopristin (Q-D) resistance probing, virulence gene (esp, hyl, efaA, asa1, gelE, cpd) detection and MLVA. Biofilm formation was evaluated by the microtiter plate method.

Results: VRE carriage prevalence among at-risk patients was 28.7%. All VRE strains were vanA positive multidrug-resistant Enterococcus faecium (VRfm), harboring ermB-1 (38.9%), esp (84%), efaA (71.2%), hyl (54.5%), asa1 (23.4%), gelE and cpd (11.6%) each. Ability of biofilm production was detected in 20.8%. Genetic relatedness of the isolates revealed 13 clusters, heterogeneous picture and 25 unique MTs profiles.

Conclusion: The obtained prevalence of VRE intestinal carriage among high-risk inpatients in Serbia is higher than the European average, with high percentage of multidrug resistance. The emergence of resistance to Q-D is of particular concern. Close monitoring of pattern of resistance and strict adherence to specific guidelines are urgently needed in Serbia.",
publisher = "BMC",
journal = "Annals of Clinical Microbiology and Antimicrobials",
title = "Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report",
pages = "18",
volume = "20",
doi = "10.1186/s12941-021-00423-0"
}
Janjusevic, A., Markovic Denic, L., Minic, R., Anita, G.,& Cirkovic, I.. (2021). Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report. in Annals of Clinical Microbiology and Antimicrobials
BMC., 20, 18.
https://doi.org/10.1186/s12941-021-00423-0
Janjusevic A, Markovic Denic L, Minic R, Anita G, Cirkovic I. Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report. in Annals of Clinical Microbiology and Antimicrobials. 2021;20:18.
doi:10.1186/s12941-021-00423-0 .
Janjusevic, Ana, Markovic Denic, Ljiljana, Minic, Rajna, Anita, Grgurevic, Cirkovic, Ivana, "Intestinal carriage of vancomycin-resistant Enterococcus spp. among high-risk patients in university hospitals in Serbia: first surveillance report" in Annals of Clinical Microbiology and Antimicrobials, 20 (2021):18,
https://doi.org/10.1186/s12941-021-00423-0 . .
3
2
2

Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity

Stanojević, Stanislava; Blagojević, Veljko; Ćuruvija, Ivana; Vujić, Vesna

(Springer Nature, 2021)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Vujić, Vesna
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/611
AB  - Gut microbiota contribute to shaping the immune repertoire of the host, whereas probiotics may exert beneficial effects by modulating immune responses. Having in mind the differences in both the composition of gut microbiota and the immune response between rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains, we investigated if intraperitoneal (i.p.) injection of live Lactobacillus rhamnosus (LB) may influence peri-toneal cavity cell response to invitro treatments with selected microbiota in the rat strain-dependent manner. Peritoneal cavity cells from AO and DA rats were lavaged two (d2) and seven days (d7) following i.p. injection with LB and tested for NO, urea, and H2O2 release basally, or upon invitro stimulation with autologous E.coli and Enterococcus spp. Whereas the single i.p. injection of LB nearly depleted resident macrophages and increased the proportion of small inflammatory macrophages and monocytes on d2 in both rat strains, greater proportion of MHCIIhiCD163− and CCR7+ cells and increased NO/diminished H2O2 release in DA compared with AO rats suggest a more intense inflammatory prim-ing by LB in this rat strain. Even though E.coli- and/or Enterococcus spp.-induced rise in H2O2 release invitro was abrogated by LB in cells from both rat strains, LB prevented microbiota-induced increase in NO/urea ratio only in cells from AO and augmented it in cells from DA rats. Thus, the immunomodulatory properties may not be constant for particular probiotic bacteria, but shaped by innate immunity of the host.
PB  - Springer Nature
T2  - Inflammation
T1  - Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity
DO  - 10.1007/s10753-021-01513-z
ER  - 
@article{
author = "Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Vujić, Vesna",
year = "2021",
abstract = "Gut microbiota contribute to shaping the immune repertoire of the host, whereas probiotics may exert beneficial effects by modulating immune responses. Having in mind the differences in both the composition of gut microbiota and the immune response between rats of Albino Oxford (AO) and Dark Agouti (DA) rat strains, we investigated if intraperitoneal (i.p.) injection of live Lactobacillus rhamnosus (LB) may influence peri-toneal cavity cell response to invitro treatments with selected microbiota in the rat strain-dependent manner. Peritoneal cavity cells from AO and DA rats were lavaged two (d2) and seven days (d7) following i.p. injection with LB and tested for NO, urea, and H2O2 release basally, or upon invitro stimulation with autologous E.coli and Enterococcus spp. Whereas the single i.p. injection of LB nearly depleted resident macrophages and increased the proportion of small inflammatory macrophages and monocytes on d2 in both rat strains, greater proportion of MHCIIhiCD163− and CCR7+ cells and increased NO/diminished H2O2 release in DA compared with AO rats suggest a more intense inflammatory prim-ing by LB in this rat strain. Even though E.coli- and/or Enterococcus spp.-induced rise in H2O2 release invitro was abrogated by LB in cells from both rat strains, LB prevented microbiota-induced increase in NO/urea ratio only in cells from AO and augmented it in cells from DA rats. Thus, the immunomodulatory properties may not be constant for particular probiotic bacteria, but shaped by innate immunity of the host.",
publisher = "Springer Nature",
journal = "Inflammation",
title = "Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity",
doi = "10.1007/s10753-021-01513-z"
}
Stanojević, S., Blagojević, V., Ćuruvija, I.,& Vujić, V.. (2021). Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity. in Inflammation
Springer Nature..
https://doi.org/10.1007/s10753-021-01513-z
Stanojević S, Blagojević V, Ćuruvija I, Vujić V. Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity. in Inflammation. 2021;.
doi:10.1007/s10753-021-01513-z .
Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Vujić, Vesna, "Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity" in Inflammation (2021),
https://doi.org/10.1007/s10753-021-01513-z . .
1
1

MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation

Milovanović, Vladimir; Minić, Rajna; Vakić, J.; Ivanović, S.; Cupić, V.; Borozan, S.; Nešić, A.; Živković, Irena

(Elsevier Ltd, 2021)

TY  - JOUR
AU  - Milovanović, Vladimir
AU  - Minić, Rajna
AU  - Vakić, J.
AU  - Ivanović, S.
AU  - Cupić, V.
AU  - Borozan, S.
AU  - Nešić, A.
AU  - Živković, Irena
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/572
AB  - The MTT assay is routinely used to detect the activity of living cells. While working with Vipera ammodytes venom we detected the reduction of MTT without the presence of cells, in a concentration-dependent manner. By combining non-reducing PAGE, L-amino acid oxidase (LAAO) assays, and standard MTT assays, we established and confirmed that venom MTT reduction is catalyzed by only one enzyme, the LAAO. Even though it was previously known that the dimeric and tetrameric forms of LAAO are active, we conclude that the enzyme is also active in the monomeric form. Our results have led to the definition of a new MTT assay in a microtiter plate for in vitro testing of svLAAO activity i.e. from the venom of the V. ammodytes snake. Potentially, this method can be used for testing hemorrhagic venoms of other snakes as well as the LAAO neutralization capability of appropriate antivenoms.
PB  - Elsevier Ltd
T2  - Toxicon
T1  - MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation
EP  - 65
SP  - 57
VL  - 192
DO  - 10.1016/j.toxicon.2021.01.012
UR  - conv_586
ER  - 
@article{
author = "Milovanović, Vladimir and Minić, Rajna and Vakić, J. and Ivanović, S. and Cupić, V. and Borozan, S. and Nešić, A. and Živković, Irena",
year = "2021",
abstract = "The MTT assay is routinely used to detect the activity of living cells. While working with Vipera ammodytes venom we detected the reduction of MTT without the presence of cells, in a concentration-dependent manner. By combining non-reducing PAGE, L-amino acid oxidase (LAAO) assays, and standard MTT assays, we established and confirmed that venom MTT reduction is catalyzed by only one enzyme, the LAAO. Even though it was previously known that the dimeric and tetrameric forms of LAAO are active, we conclude that the enzyme is also active in the monomeric form. Our results have led to the definition of a new MTT assay in a microtiter plate for in vitro testing of svLAAO activity i.e. from the venom of the V. ammodytes snake. Potentially, this method can be used for testing hemorrhagic venoms of other snakes as well as the LAAO neutralization capability of appropriate antivenoms.",
publisher = "Elsevier Ltd",
journal = "Toxicon",
title = "MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation",
pages = "65-57",
volume = "192",
doi = "10.1016/j.toxicon.2021.01.012",
url = "conv_586"
}
Milovanović, V., Minić, R., Vakić, J., Ivanović, S., Cupić, V., Borozan, S., Nešić, A.,& Živković, I.. (2021). MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation. in Toxicon
Elsevier Ltd., 192, 57-65.
https://doi.org/10.1016/j.toxicon.2021.01.012
conv_586
Milovanović V, Minić R, Vakić J, Ivanović S, Cupić V, Borozan S, Nešić A, Živković I. MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation. in Toxicon. 2021;192:57-65.
doi:10.1016/j.toxicon.2021.01.012
conv_586 .
Milovanović, Vladimir, Minić, Rajna, Vakić, J., Ivanović, S., Cupić, V., Borozan, S., Nešić, A., Živković, Irena, "MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation" in Toxicon, 192 (2021):57-65,
https://doi.org/10.1016/j.toxicon.2021.01.012 .,
conv_586 .
1
1
1
1

Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant

Kosanovic, Dejana; Dyas, Maria; Grogan, Helen; Kavanagh, Kevin

(Springer, 2021)

TY  - JOUR
AU  - Kosanovic, Dejana
AU  - Dyas, Maria
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/613
AB  - Trichoderma aggressivum, a mycopathogen causing green mould disease, is a major problem in Agaricus bisporus cultivation due to crop loss, and resistance to chemical fungicides. There is an urgent need for novel biological ways to control mycopathogens without affecting the growth of A. bisporus. Bacteria from the mushroom-casing environment were identified and tested for antagonistic effect on T. aggressivum. Bacillus velezensis produced a large zone of inhibition and its supernatant inhibited the growth of T. aggressivum [−37%], and slightly stimulated A. bisporus growth [+2%]. Label free quantitative-proteomic (LFQ) analysis of changes in the abundance of T. aggressivum proteins following exposure to B. velezensis supernatant indicated increased abundance of proteins associated with catabolic processing of amino acids (40-fold), amino oxidase proteins (14-fold), oxidoreductase proteins (13-fold, 4-fold) and hydrolases (3-fold). Proteins that decreased in relative abundance were antioxidants (29-fold), NTF2 domain containing protein (17-fold), 60S ribosomal protein L-13 (14-fold), glucoamylase proteins (13-fold), proteasome subunit proteins (11-fold) and other ribosomal proteins (9-fold). LFQ analysis revealed that exposing A. bisporus to B. velezensis supernatant led to a decrease in: prohibitin (13-fold, 6-fold), proteasomal proteins (11-fold), cytosolic adaptor domain containing protein (5-fold), aldehyde dehydrogenase (4-fold), ribosomal proteins (4-fold), DLH domain-containing protein (4-fold) and PKS_ER domain containing protein (3-fold). The results indicate that A. bisporus was not under stress upon contact with B. velezensis. Whereas a detrimental effect of B. velezensis on T. aggressivum is shown by inhibition of growth and damage-preventing proteins and increased abundance of proteins associated with stress.
PB  - Springer
T2  - European Journal of Plant Pathology
T1  - Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant
IS  - 2
VL  - 160
VL  - 397
DO  - 10.1007/s10658-021-02252-5
ER  - 
@article{
author = "Kosanovic, Dejana and Dyas, Maria and Grogan, Helen and Kavanagh, Kevin",
year = "2021",
abstract = "Trichoderma aggressivum, a mycopathogen causing green mould disease, is a major problem in Agaricus bisporus cultivation due to crop loss, and resistance to chemical fungicides. There is an urgent need for novel biological ways to control mycopathogens without affecting the growth of A. bisporus. Bacteria from the mushroom-casing environment were identified and tested for antagonistic effect on T. aggressivum. Bacillus velezensis produced a large zone of inhibition and its supernatant inhibited the growth of T. aggressivum [−37%], and slightly stimulated A. bisporus growth [+2%]. Label free quantitative-proteomic (LFQ) analysis of changes in the abundance of T. aggressivum proteins following exposure to B. velezensis supernatant indicated increased abundance of proteins associated with catabolic processing of amino acids (40-fold), amino oxidase proteins (14-fold), oxidoreductase proteins (13-fold, 4-fold) and hydrolases (3-fold). Proteins that decreased in relative abundance were antioxidants (29-fold), NTF2 domain containing protein (17-fold), 60S ribosomal protein L-13 (14-fold), glucoamylase proteins (13-fold), proteasome subunit proteins (11-fold) and other ribosomal proteins (9-fold). LFQ analysis revealed that exposing A. bisporus to B. velezensis supernatant led to a decrease in: prohibitin (13-fold, 6-fold), proteasomal proteins (11-fold), cytosolic adaptor domain containing protein (5-fold), aldehyde dehydrogenase (4-fold), ribosomal proteins (4-fold), DLH domain-containing protein (4-fold) and PKS_ER domain containing protein (3-fold). The results indicate that A. bisporus was not under stress upon contact with B. velezensis. Whereas a detrimental effect of B. velezensis on T. aggressivum is shown by inhibition of growth and damage-preventing proteins and increased abundance of proteins associated with stress.",
publisher = "Springer",
journal = "European Journal of Plant Pathology",
title = "Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant",
number = "2",
volume = "160, 397",
doi = "10.1007/s10658-021-02252-5"
}
Kosanovic, D., Dyas, M., Grogan, H.,& Kavanagh, K.. (2021). Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant. in European Journal of Plant Pathology
Springer., 160(2).
https://doi.org/10.1007/s10658-021-02252-5
Kosanovic D, Dyas M, Grogan H, Kavanagh K. Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant. in European Journal of Plant Pathology. 2021;160(2).
doi:10.1007/s10658-021-02252-5 .
Kosanovic, Dejana, Dyas, Maria, Grogan, Helen, Kavanagh, Kevin, "Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant" in European Journal of Plant Pathology, 160, no. 2 (2021),
https://doi.org/10.1007/s10658-021-02252-5 . .
2
3
3

The effect of influenza vaccine immunization on natural antibodies

Zivkovic, Irena; Muhandes, Lina; Petrusic, Vladimir; Minic, Rajna; Dimitrijevic, Ljiljana

(Pharmaceutical Association of Serbia, 2021)

TY  - JOUR
AU  - Zivkovic, Irena
AU  - Muhandes, Lina
AU  - Petrusic, Vladimir
AU  - Minic, Rajna
AU  - Dimitrijevic, Ljiljana
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/612
AB  - Natural, polyreactive, low-affinity antibodies are known to play an important role not only
in the immediate defense against pathogens, but also in shaping the acquired immune response.
On the other hand, antigen specific, high-affinity antibodies can affect the balance of natural
antibodies and lead to autoimmune diseases. In this study we have analyzed the changes that occur
in the IgM and IgG pool of natural antibodies after immunization with split or whole virion
influenza vaccine. For this purpose, “in-house” developed ELISAs were used. The subjects were
divided, according to the vaccination status, into those who had been immunized with the
influenza vaccine in previous years and those who had been immunized for the first time. The
analysis indicated that the pool of natural antibodies was not impaired by the immunization,
evidenced by the lack of changes in any of the groups, and that certain fluctuations were induced
in order to maintain the homeostasis of the immune system.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - The effect of influenza vaccine immunization on natural antibodies
EP  - 223
SP  - 207
VL  - 71
DO  - 10.5937/arhfarm71‐31544
ER  - 
@article{
author = "Zivkovic, Irena and Muhandes, Lina and Petrusic, Vladimir and Minic, Rajna and Dimitrijevic, Ljiljana",
year = "2021",
abstract = "Natural, polyreactive, low-affinity antibodies are known to play an important role not only
in the immediate defense against pathogens, but also in shaping the acquired immune response.
On the other hand, antigen specific, high-affinity antibodies can affect the balance of natural
antibodies and lead to autoimmune diseases. In this study we have analyzed the changes that occur
in the IgM and IgG pool of natural antibodies after immunization with split or whole virion
influenza vaccine. For this purpose, “in-house” developed ELISAs were used. The subjects were
divided, according to the vaccination status, into those who had been immunized with the
influenza vaccine in previous years and those who had been immunized for the first time. The
analysis indicated that the pool of natural antibodies was not impaired by the immunization,
evidenced by the lack of changes in any of the groups, and that certain fluctuations were induced
in order to maintain the homeostasis of the immune system.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "The effect of influenza vaccine immunization on natural antibodies",
pages = "223-207",
volume = "71",
doi = "10.5937/arhfarm71‐31544"
}
Zivkovic, I., Muhandes, L., Petrusic, V., Minic, R.,& Dimitrijevic, L.. (2021). The effect of influenza vaccine immunization on natural antibodies. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 71, 207-223.
https://doi.org/10.5937/arhfarm71‐31544
Zivkovic I, Muhandes L, Petrusic V, Minic R, Dimitrijevic L. The effect of influenza vaccine immunization on natural antibodies. in Arhiv za farmaciju. 2021;71:207-223.
doi:10.5937/arhfarm71‐31544 .
Zivkovic, Irena, Muhandes, Lina, Petrusic, Vladimir, Minic, Rajna, Dimitrijevic, Ljiljana, "The effect of influenza vaccine immunization on natural antibodies" in Arhiv za farmaciju, 71 (2021):207-223,
https://doi.org/10.5937/arhfarm71‐31544 . .

Safety and immunogenicity of a seasonal trivalent inactivated split influenza vaccine: a double blind, phase III randomized clinical trial in healthy Serbian adults

Stevanović, Goran; Obradović, Aleksandar; Ristić, Snezana; Petrović, Dragan; Milenković, Branislava; Mitrović, Danilo; Vignjević Filipović, Svetlana; Ilić, Katarina; Stoiljković, Vera; Lavadinović, Lidija; Pelemis, Mijomir; Petrović, Svetlana; Vidmanić, Ana; Popović, Olga; Eremić, Natasa; Sparrow, Erin; Torelli, Guido; Socquet, Muriel; Holt, Renée; Ilieva-Borisova, Yordanka; Tang, Yuxiao; Scorza, Francesco Berlanda; Flores, Jorge; Rathi, Niraj

(SAGE Publications, 2020)

TY  - JOUR
AU  - Stevanović, Goran
AU  - Obradović, Aleksandar
AU  - Ristić, Snezana
AU  - Petrović, Dragan
AU  - Milenković, Branislava
AU  - Mitrović, Danilo
AU  - Vignjević Filipović, Svetlana
AU  - Ilić, Katarina
AU  - Stoiljković, Vera
AU  - Lavadinović, Lidija
AU  - Pelemis, Mijomir
AU  - Petrović, Svetlana
AU  - Vidmanić, Ana
AU  - Popović, Olga
AU  - Eremić, Natasa
AU  - Sparrow, Erin
AU  - Torelli, Guido
AU  - Socquet, Muriel
AU  - Holt, Renée
AU  - Ilieva-Borisova, Yordanka
AU  - Tang, Yuxiao
AU  - Scorza, Francesco Berlanda
AU  - Flores, Jorge
AU  - Rathi, Niraj
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/616
AB  - This study was a phase III, multicenter, double-blind, randomized, placebo-controlled trial to evaluate the safety and immunogenicity of a seasonal trivalent split, inactivated influenza vaccine (TIV) in healthy Serbian adults between the ages of 18 and 65 years. This egg-based vaccine was manufactured by the Institute of Virology, Vaccines and Sera, Torlak, Belgrade, Serbia. A total of 480 participants were assigned randomly in a ratio of 2:1 to receive a single intramuscular dose (0.5 ml) of the vaccine (15 µg of hemagglutinin per strain) or placebo (phosphate-buffered saline). Participants were monitored for safety, including solicited and unsolicited adverse events (AEs) and serious adverse events (SAEs). No SAEs related to vaccination were reported. Injection site pain (51.3%), injection site tenderness (40.4%), tiredness (17.0%), and headache (15.1%) were the most commonly reported solicited events in the vaccine group. Incidence of related unsolicited AEs was low (1.3%) among vaccinees. Hemagglutinin inhibition (HAI) titers were measured before and 21 days after vaccination in 151 participants. Overall, HAI seroconversion rates to H1 and H3 were observed in 90.1% and 76.2% of vaccinees, respectively. For B antigen, it was 51.5%, likely due to high pre-vaccination titers. Post-vaccination seroprotection rates were in the range of 78.2–95.0% for the three antigens. Post-vaccination geometric mean titers (GMT) were at least 3.8 times higher than baseline levels for all the three strains among vaccinees. Overall, the study showed that the vaccine was safe and well tolerated, and induced a robust immune response against all three vaccine strains., ClinicalTrials.gov identifier: NCT02935192, October 17, 2016
PB  - SAGE Publications
T2  - Therapeutic Advances in Vaccines and Immunotherapy
T1  - Safety and immunogenicity of a seasonal trivalent inactivated split influenza vaccine: a double blind, phase III randomized clinical trial in healthy Serbian adults
VL  - 8
DO  - 10.1177/2515135520925336
ER  - 
@article{
author = "Stevanović, Goran and Obradović, Aleksandar and Ristić, Snezana and Petrović, Dragan and Milenković, Branislava and Mitrović, Danilo and Vignjević Filipović, Svetlana and Ilić, Katarina and Stoiljković, Vera and Lavadinović, Lidija and Pelemis, Mijomir and Petrović, Svetlana and Vidmanić, Ana and Popović, Olga and Eremić, Natasa and Sparrow, Erin and Torelli, Guido and Socquet, Muriel and Holt, Renée and Ilieva-Borisova, Yordanka and Tang, Yuxiao and Scorza, Francesco Berlanda and Flores, Jorge and Rathi, Niraj",
year = "2020",
abstract = "This study was a phase III, multicenter, double-blind, randomized, placebo-controlled trial to evaluate the safety and immunogenicity of a seasonal trivalent split, inactivated influenza vaccine (TIV) in healthy Serbian adults between the ages of 18 and 65 years. This egg-based vaccine was manufactured by the Institute of Virology, Vaccines and Sera, Torlak, Belgrade, Serbia. A total of 480 participants were assigned randomly in a ratio of 2:1 to receive a single intramuscular dose (0.5 ml) of the vaccine (15 µg of hemagglutinin per strain) or placebo (phosphate-buffered saline). Participants were monitored for safety, including solicited and unsolicited adverse events (AEs) and serious adverse events (SAEs). No SAEs related to vaccination were reported. Injection site pain (51.3%), injection site tenderness (40.4%), tiredness (17.0%), and headache (15.1%) were the most commonly reported solicited events in the vaccine group. Incidence of related unsolicited AEs was low (1.3%) among vaccinees. Hemagglutinin inhibition (HAI) titers were measured before and 21 days after vaccination in 151 participants. Overall, HAI seroconversion rates to H1 and H3 were observed in 90.1% and 76.2% of vaccinees, respectively. For B antigen, it was 51.5%, likely due to high pre-vaccination titers. Post-vaccination seroprotection rates were in the range of 78.2–95.0% for the three antigens. Post-vaccination geometric mean titers (GMT) were at least 3.8 times higher than baseline levels for all the three strains among vaccinees. Overall, the study showed that the vaccine was safe and well tolerated, and induced a robust immune response against all three vaccine strains., ClinicalTrials.gov identifier: NCT02935192, October 17, 2016",
publisher = "SAGE Publications",
journal = "Therapeutic Advances in Vaccines and Immunotherapy",
title = "Safety and immunogenicity of a seasonal trivalent inactivated split influenza vaccine: a double blind, phase III randomized clinical trial in healthy Serbian adults",
volume = "8",
doi = "10.1177/2515135520925336"
}
Stevanović, G., Obradović, A., Ristić, S., Petrović, D., Milenković, B., Mitrović, D., Vignjević Filipović, S., Ilić, K., Stoiljković, V., Lavadinović, L., Pelemis, M., Petrović, S., Vidmanić, A., Popović, O., Eremić, N., Sparrow, E., Torelli, G., Socquet, M., Holt, R., Ilieva-Borisova, Y., Tang, Y., Scorza, F. B., Flores, J.,& Rathi, N.. (2020). Safety and immunogenicity of a seasonal trivalent inactivated split influenza vaccine: a double blind, phase III randomized clinical trial in healthy Serbian adults. in Therapeutic Advances in Vaccines and Immunotherapy
SAGE Publications., 8.
https://doi.org/10.1177/2515135520925336
Stevanović G, Obradović A, Ristić S, Petrović D, Milenković B, Mitrović D, Vignjević Filipović S, Ilić K, Stoiljković V, Lavadinović L, Pelemis M, Petrović S, Vidmanić A, Popović O, Eremić N, Sparrow E, Torelli G, Socquet M, Holt R, Ilieva-Borisova Y, Tang Y, Scorza FB, Flores J, Rathi N. Safety and immunogenicity of a seasonal trivalent inactivated split influenza vaccine: a double blind, phase III randomized clinical trial in healthy Serbian adults. in Therapeutic Advances in Vaccines and Immunotherapy. 2020;8.
doi:10.1177/2515135520925336 .
Stevanović, Goran, Obradović, Aleksandar, Ristić, Snezana, Petrović, Dragan, Milenković, Branislava, Mitrović, Danilo, Vignjević Filipović, Svetlana, Ilić, Katarina, Stoiljković, Vera, Lavadinović, Lidija, Pelemis, Mijomir, Petrović, Svetlana, Vidmanić, Ana, Popović, Olga, Eremić, Natasa, Sparrow, Erin, Torelli, Guido, Socquet, Muriel, Holt, Renée, Ilieva-Borisova, Yordanka, Tang, Yuxiao, Scorza, Francesco Berlanda, Flores, Jorge, Rathi, Niraj, "Safety and immunogenicity of a seasonal trivalent inactivated split influenza vaccine: a double blind, phase III randomized clinical trial in healthy Serbian adults" in Therapeutic Advances in Vaccines and Immunotherapy, 8 (2020),
https://doi.org/10.1177/2515135520925336 . .

Role of the End-Point Mediators of Sympathoadrenal and Sympathoneural Stress Axes in the Pathogenesis of Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis

Pilipović, Ivan; Stojić-Vukanić, Zorica; Prijić, Ivana; Leposavić, Gordana

(Frontiers Media Sa, Lausanne, 2020)

TY  - JOUR
AU  - Pilipović, Ivan
AU  - Stojić-Vukanić, Zorica
AU  - Prijić, Ivana
AU  - Leposavić, Gordana
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/571
AB  - The role of stress effector systems in the initiation and progression of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE), the most commonly used experimental model of MS, has strongly been suggested. To corroborate this notion, alterations in activity of the sympathoadrenal and sympathoneural axes of sympathoadrenal system (a major communication pathway between the central nervous system and the immune system), mirrored in altered release of their end-point mediators (adrenaline and noradrenaline, respectively), are shown to precede (in MS) and/or occur during development of MS and EAE in response to immune cell activation (in early phase of disease) and disease-related damage of sympathoadrenal system neurons and their projections (in late phase of disease). To add to the complexity, innate immunity cells and T-lymphocytes synthesize noradrenaline that may be implicated in a local autocrine/paracrine self-amplifying feed-forward loop to enhance myeloid-cell synthesis of proinflammatory cytokines and inflammatory injury. Furthermore, experimental manipulations targeting noradrenaline/adrenaline action are shown to influence clinical outcome of EAE, in a disease phase-specific manner. This is partly related to the fact that virtually all types of cells involved in the instigation and progression of autoimmune inflammation and target tissue damage in EAE/MS express functional adrenoceptors. Although catecholamines exert majority of immunomodulatory effects through beta(2)-adrenoceptor, a role for alpha-adrenoceptors in EAE pathogenesis has also been indicated. In this review, we summarize all aforementioned aspects of immunopathogenetic action of catecholamines in EAE/MS as possibly important for designing new strategies targeting their action to prevent/mitigate autoimmune neuroinflammation and tissue damage.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Endocrinology
T1  - Role of the End-Point Mediators of Sympathoadrenal and Sympathoneural Stress Axes in the Pathogenesis of Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis
VL  - 10
DO  - 10.3389/fendo.2019.00921
UR  - conv_467
ER  - 
@article{
author = "Pilipović, Ivan and Stojić-Vukanić, Zorica and Prijić, Ivana and Leposavić, Gordana",
year = "2020",
abstract = "The role of stress effector systems in the initiation and progression of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE), the most commonly used experimental model of MS, has strongly been suggested. To corroborate this notion, alterations in activity of the sympathoadrenal and sympathoneural axes of sympathoadrenal system (a major communication pathway between the central nervous system and the immune system), mirrored in altered release of their end-point mediators (adrenaline and noradrenaline, respectively), are shown to precede (in MS) and/or occur during development of MS and EAE in response to immune cell activation (in early phase of disease) and disease-related damage of sympathoadrenal system neurons and their projections (in late phase of disease). To add to the complexity, innate immunity cells and T-lymphocytes synthesize noradrenaline that may be implicated in a local autocrine/paracrine self-amplifying feed-forward loop to enhance myeloid-cell synthesis of proinflammatory cytokines and inflammatory injury. Furthermore, experimental manipulations targeting noradrenaline/adrenaline action are shown to influence clinical outcome of EAE, in a disease phase-specific manner. This is partly related to the fact that virtually all types of cells involved in the instigation and progression of autoimmune inflammation and target tissue damage in EAE/MS express functional adrenoceptors. Although catecholamines exert majority of immunomodulatory effects through beta(2)-adrenoceptor, a role for alpha-adrenoceptors in EAE pathogenesis has also been indicated. In this review, we summarize all aforementioned aspects of immunopathogenetic action of catecholamines in EAE/MS as possibly important for designing new strategies targeting their action to prevent/mitigate autoimmune neuroinflammation and tissue damage.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Endocrinology",
title = "Role of the End-Point Mediators of Sympathoadrenal and Sympathoneural Stress Axes in the Pathogenesis of Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis",
volume = "10",
doi = "10.3389/fendo.2019.00921",
url = "conv_467"
}
Pilipović, I., Stojić-Vukanić, Z., Prijić, I.,& Leposavić, G.. (2020). Role of the End-Point Mediators of Sympathoadrenal and Sympathoneural Stress Axes in the Pathogenesis of Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis. in Frontiers in Endocrinology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fendo.2019.00921
conv_467
Pilipović I, Stojić-Vukanić Z, Prijić I, Leposavić G. Role of the End-Point Mediators of Sympathoadrenal and Sympathoneural Stress Axes in the Pathogenesis of Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis. in Frontiers in Endocrinology. 2020;10.
doi:10.3389/fendo.2019.00921
conv_467 .
Pilipović, Ivan, Stojić-Vukanić, Zorica, Prijić, Ivana, Leposavić, Gordana, "Role of the End-Point Mediators of Sympathoadrenal and Sympathoneural Stress Axes in the Pathogenesis of Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis" in Frontiers in Endocrinology, 10 (2020),
https://doi.org/10.3389/fendo.2019.00921 .,
conv_467 .
1
1
1
1

A Forgotten Episode of Marburg Virus Disease: Belgrade, Yugoslavia, 1967

Ristanović, Elizabeta; Kokoskov, Nenad S.; Crozier, Ian; Kuhn, Jens H.; Gligić, Ana

(Amer Soc Microbiology, Washington, 2020)

TY  - JOUR
AU  - Ristanović, Elizabeta
AU  - Kokoskov, Nenad S.
AU  - Crozier, Ian
AU  - Kuhn, Jens H.
AU  - Gligić, Ana
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/570
AB  - In 1967, several workers involved in poliomyelitis vaccine development and production fell ill at three different locations in Europe with a severe and often lethal novel disease associated with grivets (Chlorocebus aethiops) imported from Uganda. This disease was named Marburg virus disease (MVD) after the West German town of Marburg an der Lahn, where most human infections and deaths had been recorded. Consequently, the Marburg episode received the most scientific and media attention. Cases that occurred in Frankfurt am Main, West Germany, were also described in commonly accessible scientific literature, although they were less frequently cited than those pertaining to the Marburg infections. However, two infections occurring in a third location, in Belgrade, Yugoslavia, have seemingly been all but forgotten. Due in part to their absence in commonly used databases and in part to the fact that they were written in languages other than English, the important articles describing this part of the outbreak are very rarely cited. Here, we summarize this literature and correct published inaccuracies to remind a younger generation of scientists focusing on Marburg virus and its closest filoviral relatives of this important historical context. Importantly, and unfortunately, the three episodes of infection of 1967 still represent the best in-depth clinical look at MVD in general and in the context of "modern" medicine (fully resourced versus less-resourced capacity) in particular. Hence, each individual case of these episodes holds crucial information for health care providers who may be confronted with MVD today.
PB  - Amer Soc Microbiology, Washington
T2  - Microbiology and Molecular Biology Reviews
T1  - A Forgotten Episode of Marburg Virus Disease: Belgrade, Yugoslavia, 1967
IS  - 2
VL  - 84
DO  - 10.1128/MMBR.00095-19
UR  - conv_476
ER  - 
@article{
author = "Ristanović, Elizabeta and Kokoskov, Nenad S. and Crozier, Ian and Kuhn, Jens H. and Gligić, Ana",
year = "2020",
abstract = "In 1967, several workers involved in poliomyelitis vaccine development and production fell ill at three different locations in Europe with a severe and often lethal novel disease associated with grivets (Chlorocebus aethiops) imported from Uganda. This disease was named Marburg virus disease (MVD) after the West German town of Marburg an der Lahn, where most human infections and deaths had been recorded. Consequently, the Marburg episode received the most scientific and media attention. Cases that occurred in Frankfurt am Main, West Germany, were also described in commonly accessible scientific literature, although they were less frequently cited than those pertaining to the Marburg infections. However, two infections occurring in a third location, in Belgrade, Yugoslavia, have seemingly been all but forgotten. Due in part to their absence in commonly used databases and in part to the fact that they were written in languages other than English, the important articles describing this part of the outbreak are very rarely cited. Here, we summarize this literature and correct published inaccuracies to remind a younger generation of scientists focusing on Marburg virus and its closest filoviral relatives of this important historical context. Importantly, and unfortunately, the three episodes of infection of 1967 still represent the best in-depth clinical look at MVD in general and in the context of "modern" medicine (fully resourced versus less-resourced capacity) in particular. Hence, each individual case of these episodes holds crucial information for health care providers who may be confronted with MVD today.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Microbiology and Molecular Biology Reviews",
title = "A Forgotten Episode of Marburg Virus Disease: Belgrade, Yugoslavia, 1967",
number = "2",
volume = "84",
doi = "10.1128/MMBR.00095-19",
url = "conv_476"
}
Ristanović, E., Kokoskov, N. S., Crozier, I., Kuhn, J. H.,& Gligić, A.. (2020). A Forgotten Episode of Marburg Virus Disease: Belgrade, Yugoslavia, 1967. in Microbiology and Molecular Biology Reviews
Amer Soc Microbiology, Washington., 84(2).
https://doi.org/10.1128/MMBR.00095-19
conv_476
Ristanović E, Kokoskov NS, Crozier I, Kuhn JH, Gligić A. A Forgotten Episode of Marburg Virus Disease: Belgrade, Yugoslavia, 1967. in Microbiology and Molecular Biology Reviews. 2020;84(2).
doi:10.1128/MMBR.00095-19
conv_476 .
Ristanović, Elizabeta, Kokoskov, Nenad S., Crozier, Ian, Kuhn, Jens H., Gligić, Ana, "A Forgotten Episode of Marburg Virus Disease: Belgrade, Yugoslavia, 1967" in Microbiology and Molecular Biology Reviews, 84, no. 2 (2020),
https://doi.org/10.1128/MMBR.00095-19 .,
conv_476 .
13
18
4
14

Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner

Lukić, Jovanka; Stanisavljević, Nemanja; Vukotić, Goran; Kosanović, Dejana; Terzić-Vidojević, Amarela; Begović, Jelena; Golić, Nataša; Jeney, Galina; Ljubobratović, Uroš

(Elsevier, Amsterdam, 2020)

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kosanović, Dejana
AU  - Terzić-Vidojević, Amarela
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Jeney, Galina
AU  - Ljubobratović, Uroš
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/569
AB  - This study aimed to analyze an impact of Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 supplemented to Anemia franciscana cultivation medium on biochemical profile of hatched nauplii. Impacts of different BGHO1:BGGO6-55 ratios (75:25, 50:50 and 25:75) and their total concentrations (250 and 500 g/kg of Anemia cysts) at different timings of bacteria application (pre- and post-hatching) were examined. The effects were evaluated by quantification of naupliar peptide, soluble protein, phospho-, neutral and unsaturated lipid content. Uni and multifactorial analysis of variance (ANOVA) were used to estimate the effects of treatments relative to control and to model factor interactions, respectively Statistical analysis indicated that posthatching application of 75:25 strain ratio at high dose was associated with an increase of neutral lipid amount. Furthermore, factor interaction profiling identified positive correlation of lacto-bacilli concentration with the level of free amino acids/short peptides, phospho-, neutral and unsaturated lipids, but only at 50:50 strain ratio combination. Application of lower lactobacilli dose at 75:25 strain ratio caused an increase of soluble protein and phospholipid amount. Hypothetically, graded response of Artemia nauplii to lactobacilli supplementation was induced with different BGHO1 doses. It assumedly ranged from stress-response protein synthesis at lower doses to membrane permeability alterations and triglyceride-mediated defense mechanism activation at higher BGHO1 doses. In contrast to lactobacilli supplementation after Anemia hatching, pre-hatching application of lactobacilli was not associated with an increase of Anemia nutritive profile, though again there was a positive association of lactobacilli concentration with nutrient amount. This implicates interference of lactobacilli with the hatching process. Results presented here facilitate the design of future studies aiming to modify Artemia nutritive profile in accordance with nutritional demands of cultivated fish species.
PB  - Elsevier, Amsterdam
T2  - Animal Feed Science and Technology
T1  - Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner
VL  - 259
DO  - 10.1016/j.anifeedsci.2019.114356
UR  - conv_475
ER  - 
@article{
author = "Lukić, Jovanka and Stanisavljević, Nemanja and Vukotić, Goran and Kosanović, Dejana and Terzić-Vidojević, Amarela and Begović, Jelena and Golić, Nataša and Jeney, Galina and Ljubobratović, Uroš",
year = "2020",
abstract = "This study aimed to analyze an impact of Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 supplemented to Anemia franciscana cultivation medium on biochemical profile of hatched nauplii. Impacts of different BGHO1:BGGO6-55 ratios (75:25, 50:50 and 25:75) and their total concentrations (250 and 500 g/kg of Anemia cysts) at different timings of bacteria application (pre- and post-hatching) were examined. The effects were evaluated by quantification of naupliar peptide, soluble protein, phospho-, neutral and unsaturated lipid content. Uni and multifactorial analysis of variance (ANOVA) were used to estimate the effects of treatments relative to control and to model factor interactions, respectively Statistical analysis indicated that posthatching application of 75:25 strain ratio at high dose was associated with an increase of neutral lipid amount. Furthermore, factor interaction profiling identified positive correlation of lacto-bacilli concentration with the level of free amino acids/short peptides, phospho-, neutral and unsaturated lipids, but only at 50:50 strain ratio combination. Application of lower lactobacilli dose at 75:25 strain ratio caused an increase of soluble protein and phospholipid amount. Hypothetically, graded response of Artemia nauplii to lactobacilli supplementation was induced with different BGHO1 doses. It assumedly ranged from stress-response protein synthesis at lower doses to membrane permeability alterations and triglyceride-mediated defense mechanism activation at higher BGHO1 doses. In contrast to lactobacilli supplementation after Anemia hatching, pre-hatching application of lactobacilli was not associated with an increase of Anemia nutritive profile, though again there was a positive association of lactobacilli concentration with nutrient amount. This implicates interference of lactobacilli with the hatching process. Results presented here facilitate the design of future studies aiming to modify Artemia nutritive profile in accordance with nutritional demands of cultivated fish species.",
publisher = "Elsevier, Amsterdam",
journal = "Animal Feed Science and Technology",
title = "Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner",
volume = "259",
doi = "10.1016/j.anifeedsci.2019.114356",
url = "conv_475"
}
Lukić, J., Stanisavljević, N., Vukotić, G., Kosanović, D., Terzić-Vidojević, A., Begović, J., Golić, N., Jeney, G.,& Ljubobratović, U.. (2020). Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner. in Animal Feed Science and Technology
Elsevier, Amsterdam., 259.
https://doi.org/10.1016/j.anifeedsci.2019.114356
conv_475
Lukić J, Stanisavljević N, Vukotić G, Kosanović D, Terzić-Vidojević A, Begović J, Golić N, Jeney G, Ljubobratović U. Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner. in Animal Feed Science and Technology. 2020;259.
doi:10.1016/j.anifeedsci.2019.114356
conv_475 .
Lukić, Jovanka, Stanisavljević, Nemanja, Vukotić, Goran, Kosanović, Dejana, Terzić-Vidojević, Amarela, Begović, Jelena, Golić, Nataša, Jeney, Galina, Ljubobratović, Uroš, "Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner" in Animal Feed Science and Technology, 259 (2020),
https://doi.org/10.1016/j.anifeedsci.2019.114356 .,
conv_475 .
3
2
3

Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells

Obradović, Ana; Matić, Miloš; Ognjanović, Branka; Đurđević, Predrag; Marinković, Emilija; Ušćumlić, Gordana; Božić, Bojan; Božić-Nedeljković, Biljana

(Elsevier, Amsterdam, 2020)

TY  - JOUR
AU  - Obradović, Ana
AU  - Matić, Miloš
AU  - Ognjanović, Branka
AU  - Đurđević, Predrag
AU  - Marinković, Emilija
AU  - Ušćumlić, Gordana
AU  - Božić, Bojan
AU  - Božić-Nedeljković, Biljana
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/566
AB  - In this study, a series of synthesized 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives as a potential antiproliferative and antimigratory agents were investigated. The possible antitumor mechanisms of investigated hydantoin derivatives were examined on human breast cancer cell line MDA-MB-231. The cells were treated with different concentrations of compounds (from 0.01 mu M to 100 mu M) during 24 h and 72 h. The proliferation index, nitric oxide production, apoptosis rate, and migration capacity were measured. The cell invasion potential was examined by measuring the level of MMP-9 and COX-2 gene expression. All tested compounds expressed antiproliferative activity and induced dose- and time-dependent increase in the level of nitrites. The investigated molecules significantly decreased cell survival rate, migration capacity and the expression levels of genes included in the process of tumor invasion. Obtained data suggest that the tested hydantoin derivatives express considerable antitumor activity by reducing cell division rate, elevating apoptosis level, and inhibiting the motility and invasiveness of breast cancer cells. The results obtained in this study indicate that investigated compounds express potential as a novel chemotherapeutic agents against breast cancer growth and progression. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
PB  - Elsevier, Amsterdam
T2  - Saudi Pharmaceutical Journal
T1  - Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells
EP  - 254
IS  - 3
SP  - 246
VL  - 28
DO  - 10.1016/j.jsps.2020.01.003
UR  - conv_472
ER  - 
@article{
author = "Obradović, Ana and Matić, Miloš and Ognjanović, Branka and Đurđević, Predrag and Marinković, Emilija and Ušćumlić, Gordana and Božić, Bojan and Božić-Nedeljković, Biljana",
year = "2020",
abstract = "In this study, a series of synthesized 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives as a potential antiproliferative and antimigratory agents were investigated. The possible antitumor mechanisms of investigated hydantoin derivatives were examined on human breast cancer cell line MDA-MB-231. The cells were treated with different concentrations of compounds (from 0.01 mu M to 100 mu M) during 24 h and 72 h. The proliferation index, nitric oxide production, apoptosis rate, and migration capacity were measured. The cell invasion potential was examined by measuring the level of MMP-9 and COX-2 gene expression. All tested compounds expressed antiproliferative activity and induced dose- and time-dependent increase in the level of nitrites. The investigated molecules significantly decreased cell survival rate, migration capacity and the expression levels of genes included in the process of tumor invasion. Obtained data suggest that the tested hydantoin derivatives express considerable antitumor activity by reducing cell division rate, elevating apoptosis level, and inhibiting the motility and invasiveness of breast cancer cells. The results obtained in this study indicate that investigated compounds express potential as a novel chemotherapeutic agents against breast cancer growth and progression. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Pharmaceutical Journal",
title = "Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells",
pages = "254-246",
number = "3",
volume = "28",
doi = "10.1016/j.jsps.2020.01.003",
url = "conv_472"
}
Obradović, A., Matić, M., Ognjanović, B., Đurđević, P., Marinković, E., Ušćumlić, G., Božić, B.,& Božić-Nedeljković, B.. (2020). Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells. in Saudi Pharmaceutical Journal
Elsevier, Amsterdam., 28(3), 246-254.
https://doi.org/10.1016/j.jsps.2020.01.003
conv_472
Obradović A, Matić M, Ognjanović B, Đurđević P, Marinković E, Ušćumlić G, Božić B, Božić-Nedeljković B. Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells. in Saudi Pharmaceutical Journal. 2020;28(3):246-254.
doi:10.1016/j.jsps.2020.01.003
conv_472 .
Obradović, Ana, Matić, Miloš, Ognjanović, Branka, Đurđević, Predrag, Marinković, Emilija, Ušćumlić, Gordana, Božić, Bojan, Božić-Nedeljković, Biljana, "Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells" in Saudi Pharmaceutical Journal, 28, no. 3 (2020):246-254,
https://doi.org/10.1016/j.jsps.2020.01.003 .,
conv_472 .
3
3
3

Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa

Minić, Rajna; Josipović, Mirjana; Tomić-Spirić, Vesna; Gavrović-Jankulović, Marija; Perić-Popadić, Aleksandra; Prokopijević, Ivana; Ljubičić, Ana; Stamenković, Danijela; Burazer, Lidija

(MDPI, Basel, 2020)

TY  - JOUR
AU  - Minić, Rajna
AU  - Josipović, Mirjana
AU  - Tomić-Spirić, Vesna
AU  - Gavrović-Jankulović, Marija
AU  - Perić-Popadić, Aleksandra
AU  - Prokopijević, Ivana
AU  - Ljubičić, Ana
AU  - Stamenković, Danijela
AU  - Burazer, Lidija
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/565
AB  - Background and objectives: The relationship between air pollen quantity and the sensitization of allergic patients is crucial for both the diagnosis and treatment of allergic diseases. Weather conditions influence the distribution of allergenic pollen and increases in pollen concentration may negatively affect the health of allergic patients. The aim of this study was to analyze the implementation of allergen immunotherapy with regard to air pollen concentration. Material and Methods: Here we examined the relationship between Betula air pollen concentration and the usage of Betula verrucosa allergen immunotherapy in Serbia. Examination covered the period from 2015 to 2018. Measurement of airborne pollen concentration was performed with Lanzoni volumetric pollen traps. The evidence of the usage of sublingual allergen immunotherapy (SLIT) was gathered from patients with documented sensitization to specific pollen. Results: During this period tree pollens were represented with 58% +/- 21% of all measured air pollen species, while Betula pollen represented 15% +/- 8% of all tree pollens. Betula pollination peaked in April. Allergen immunotherapy to Betula verrucosa in Serbia is entirely conducted as sublingual immunotherapy and represents 47.1% +/- 1.4% of issued tree pollen SLIT. The use of pollen SLIT increased by 68% from 2015 to 2018, with an even greater increase in usage recorded for Betula SLIT-80%. Conclusions: This analysis shows a clear causative relationship between pollination and the type/prevalence of applied allergen immunotherapy. Information about the flowering seasons of allergenic plants is very important for people who suffer from allergy, for clinical allergologists, as well as for governing authorities. The presented data is of practical importance to the proper timing of immunotherapy initiation and of importance for urban landscaping. The obtained data can be the starting point for the instatement of a thorough epidemiological study and the inclusion of Serbia on the pollen map of Europe.
PB  - MDPI, Basel
T2  - Medicina-Lithuania
T1  - Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa
IS  - 2
VL  - 56
DO  - 10.3390/medicina56020059
UR  - conv_471
ER  - 
@article{
author = "Minić, Rajna and Josipović, Mirjana and Tomić-Spirić, Vesna and Gavrović-Jankulović, Marija and Perić-Popadić, Aleksandra and Prokopijević, Ivana and Ljubičić, Ana and Stamenković, Danijela and Burazer, Lidija",
year = "2020",
abstract = "Background and objectives: The relationship between air pollen quantity and the sensitization of allergic patients is crucial for both the diagnosis and treatment of allergic diseases. Weather conditions influence the distribution of allergenic pollen and increases in pollen concentration may negatively affect the health of allergic patients. The aim of this study was to analyze the implementation of allergen immunotherapy with regard to air pollen concentration. Material and Methods: Here we examined the relationship between Betula air pollen concentration and the usage of Betula verrucosa allergen immunotherapy in Serbia. Examination covered the period from 2015 to 2018. Measurement of airborne pollen concentration was performed with Lanzoni volumetric pollen traps. The evidence of the usage of sublingual allergen immunotherapy (SLIT) was gathered from patients with documented sensitization to specific pollen. Results: During this period tree pollens were represented with 58% +/- 21% of all measured air pollen species, while Betula pollen represented 15% +/- 8% of all tree pollens. Betula pollination peaked in April. Allergen immunotherapy to Betula verrucosa in Serbia is entirely conducted as sublingual immunotherapy and represents 47.1% +/- 1.4% of issued tree pollen SLIT. The use of pollen SLIT increased by 68% from 2015 to 2018, with an even greater increase in usage recorded for Betula SLIT-80%. Conclusions: This analysis shows a clear causative relationship between pollination and the type/prevalence of applied allergen immunotherapy. Information about the flowering seasons of allergenic plants is very important for people who suffer from allergy, for clinical allergologists, as well as for governing authorities. The presented data is of practical importance to the proper timing of immunotherapy initiation and of importance for urban landscaping. The obtained data can be the starting point for the instatement of a thorough epidemiological study and the inclusion of Serbia on the pollen map of Europe.",
publisher = "MDPI, Basel",
journal = "Medicina-Lithuania",
title = "Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa",
number = "2",
volume = "56",
doi = "10.3390/medicina56020059",
url = "conv_471"
}
Minić, R., Josipović, M., Tomić-Spirić, V., Gavrović-Jankulović, M., Perić-Popadić, A., Prokopijević, I., Ljubičić, A., Stamenković, D.,& Burazer, L.. (2020). Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa. in Medicina-Lithuania
MDPI, Basel., 56(2).
https://doi.org/10.3390/medicina56020059
conv_471
Minić R, Josipović M, Tomić-Spirić V, Gavrović-Jankulović M, Perić-Popadić A, Prokopijević I, Ljubičić A, Stamenković D, Burazer L. Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa. in Medicina-Lithuania. 2020;56(2).
doi:10.3390/medicina56020059
conv_471 .
Minić, Rajna, Josipović, Mirjana, Tomić-Spirić, Vesna, Gavrović-Jankulović, Marija, Perić-Popadić, Aleksandra, Prokopijević, Ivana, Ljubičić, Ana, Stamenković, Danijela, Burazer, Lidija, "Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa" in Medicina-Lithuania, 56, no. 2 (2020),
https://doi.org/10.3390/medicina56020059 .,
conv_471 .
1
1
1
1

Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis

Inić-Kanada, Aleksandra; Stojanović, Marijana; Miljković, Radmila; Stein, Elisabeth; Filipović, Ana; Frohns, Antonia; Zoeller, Nadja; Kuratli, Jasmin; Barisani-Asenbauer, Talin; Borel, Nicole

(Elsevier Science Sa, Lausanne, 2020)

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Miljković, Radmila
AU  - Stein, Elisabeth
AU  - Filipović, Ana
AU  - Frohns, Antonia
AU  - Zoeller, Nadja
AU  - Kuratli, Jasmin
AU  - Barisani-Asenbauer, Talin
AU  - Borel, Nicole
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/564
AB  - Trachoma is a devastating neglected tropical disease caused by Chlamydia trachomatis and the leading global cause of infectious blindness. Although antibiotic treatment against trachoma is efficient (SAFE strategy), additional affordable therapeutic strategies are of high interest. Water-filtered infrared A and visible light (wIRA/VIS) irradiation has proven to reduce chlamydial infectivity in vitro and ex vivo. The aim of this study was to evaluate whether wIRA/VIS can reduce chlamydial infection load and/or ocular pathology in vivo, in a guinea pig model of inclusion conjunctivitis. Guinea pigs were infected with 1 x 10(6) inclusion-forming units/eye of Chlamydia caviae via the ocular conjunctiva on day 0. In infected animals, wIRA/VIS irradiation (2100 W/m(2)) was applied on day 2 (single treatment) and on days 2 and 4 (double treatment) post-infection (pi). wIRA/VIS reduced the clinical pathology score on days 7 and 14 pi and the conjunctival chlamydial load on days 2, 4, 7, and 14 pi in comparison with C. caviae-infected, not irradiated, controls. Furthermore, numbers of chlamydial inclusions were decreased in wIRA/VIS treated C. caviae-infected guinea pigs on day 21 pi compared to C. caviae-infected, non-irradiated, controls. Double treatment with wIRA/VIS (days 2 and 4 pi) was more efficient than a single treatment on day 2 pi. wIRA/VIS treatment did neither induce macroscopic nor histologic changes in ocular tissues. Our results indicate that wIRA/VIS shows promising efficacy to reduce chlamydial infectivity in vivo without causing irradiation related pathologies in the follow-up period. wIRA/VIS irradiation is a promising approach to reduce trachoma transmission and pathology of ocular chlamydial infection.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry and Photobiology B-Biology
T1  - Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis
VL  - 209
DO  - 10.1016/j.jphotobiol.2020.111953
UR  - conv_477
ER  - 
@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Miljković, Radmila and Stein, Elisabeth and Filipović, Ana and Frohns, Antonia and Zoeller, Nadja and Kuratli, Jasmin and Barisani-Asenbauer, Talin and Borel, Nicole",
year = "2020",
abstract = "Trachoma is a devastating neglected tropical disease caused by Chlamydia trachomatis and the leading global cause of infectious blindness. Although antibiotic treatment against trachoma is efficient (SAFE strategy), additional affordable therapeutic strategies are of high interest. Water-filtered infrared A and visible light (wIRA/VIS) irradiation has proven to reduce chlamydial infectivity in vitro and ex vivo. The aim of this study was to evaluate whether wIRA/VIS can reduce chlamydial infection load and/or ocular pathology in vivo, in a guinea pig model of inclusion conjunctivitis. Guinea pigs were infected with 1 x 10(6) inclusion-forming units/eye of Chlamydia caviae via the ocular conjunctiva on day 0. In infected animals, wIRA/VIS irradiation (2100 W/m(2)) was applied on day 2 (single treatment) and on days 2 and 4 (double treatment) post-infection (pi). wIRA/VIS reduced the clinical pathology score on days 7 and 14 pi and the conjunctival chlamydial load on days 2, 4, 7, and 14 pi in comparison with C. caviae-infected, not irradiated, controls. Furthermore, numbers of chlamydial inclusions were decreased in wIRA/VIS treated C. caviae-infected guinea pigs on day 21 pi compared to C. caviae-infected, non-irradiated, controls. Double treatment with wIRA/VIS (days 2 and 4 pi) was more efficient than a single treatment on day 2 pi. wIRA/VIS treatment did neither induce macroscopic nor histologic changes in ocular tissues. Our results indicate that wIRA/VIS shows promising efficacy to reduce chlamydial infectivity in vivo without causing irradiation related pathologies in the follow-up period. wIRA/VIS irradiation is a promising approach to reduce trachoma transmission and pathology of ocular chlamydial infection.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry and Photobiology B-Biology",
title = "Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis",
volume = "209",
doi = "10.1016/j.jphotobiol.2020.111953",
url = "conv_477"
}
Inić-Kanada, A., Stojanović, M., Miljković, R., Stein, E., Filipović, A., Frohns, A., Zoeller, N., Kuratli, J., Barisani-Asenbauer, T.,& Borel, N.. (2020). Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis. in Journal of Photochemistry and Photobiology B-Biology
Elsevier Science Sa, Lausanne., 209.
https://doi.org/10.1016/j.jphotobiol.2020.111953
conv_477
Inić-Kanada A, Stojanović M, Miljković R, Stein E, Filipović A, Frohns A, Zoeller N, Kuratli J, Barisani-Asenbauer T, Borel N. Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis. in Journal of Photochemistry and Photobiology B-Biology. 2020;209.
doi:10.1016/j.jphotobiol.2020.111953
conv_477 .
Inić-Kanada, Aleksandra, Stojanović, Marijana, Miljković, Radmila, Stein, Elisabeth, Filipović, Ana, Frohns, Antonia, Zoeller, Nadja, Kuratli, Jasmin, Barisani-Asenbauer, Talin, Borel, Nicole, "Water-filtered Infrared A and visible light (wIRA/VIS) treatment reduces Chlamydia caviae-induced ocular inflammation and infectious load in a Guinea pig model of inclusion conjunctivitis" in Journal of Photochemistry and Photobiology B-Biology, 209 (2020),
https://doi.org/10.1016/j.jphotobiol.2020.111953 .,
conv_477 .
7
3
4