TY  - CONF
AU  - Kovačević, Ana
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Miljković, Radmila
AU  - Inic-Kanada, Aleksandra
AU  - Spasojević, Dragica
AU  - Radotić, Ksenija
AU  - Stojanović, Marijana M.
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/869
AB  - The dehydrogenate polymer from coniferyl alcohol (DHP; a lignin model compound) in alginate hydrogel (ALG) has been shown to exert a strong antibacterial activity. To broadens a spectrum of potential DHP/ALG application, we aimed this study to evaluate the immunomodulatory activity of DHP/ALG. DHP and ALG were tested separately and in mixture (1:2 w/w) for their impact on in vitro production of cytokines (IL-6, IL-12, and IL-10) and reactive oxygen (ROS) and nitrogen (RNS) species by resident (RMs) and thioglycolate-elicited (TGMs) peritoneal macrophages of BALB/c mice. RMs and TGMs were stimulated (48h) with ALG and DHP in concentrations previously shown to be non-cytotoxic (up to 50 and 25 μg/ml, respectively). DHP/ALG promotes simultaneous production of inflammatory (IL-6, IL-12) and regulatory cytokines by RMs in a positive dose-dependent manner. Production of inflammatory cytokines was stimulated by ALG, while an increase in IL-10 production positively correlated to the concentration of DHP. ALG also stimulated the production of IL-12 by TGMs, which was mirrored in the outcome of ALG/DHP stimulation. The significant increase in the activity of myeloperoxidase (MPO) due to DHP and/or ALG stimulation was recorded in TGMs, while a slight increase in MPO activity in RMs was recorded only upon stimulation with the higher amount of ALG. ALG in a positive dose-dependent manner stimulated the production of ROS and RNS by both RMS and TGMs. In all cases, except ROS production by RMs, the impact of ALG stimulation was mirrored in the outcome of ALG/DHP stimulation. Our results suggest that DHP/ALG exerts an immunomodulatory activity that could complement already reported antimicrobial activity and warrants further investigation on the use of DHP/ALG in the treatment of infectious diseases.
PB  - Institute for Biological Research "Siniša Stanković"
C3  - Immunology at the confluence of multidisciplinary approaches
T1  - Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate
SP  - 129
ER  - 

@conference{
author = "Kovačević, Ana and Lukić, Ivana and Marinković, Emilija and Miljković, Radmila and Inic-Kanada, Aleksandra and Spasojević, Dragica and Radotić, Ksenija and Stojanović, Marijana M.",
year = "2019",
abstract = "The dehydrogenate polymer from coniferyl alcohol (DHP; a lignin model compound) in alginate hydrogel (ALG) has been shown to exert a strong antibacterial activity. To broadens a spectrum of potential DHP/ALG application, we aimed this study to evaluate the immunomodulatory activity of DHP/ALG. DHP and ALG were tested separately and in mixture (1:2 w/w) for their impact on in vitro production of cytokines (IL-6, IL-12, and IL-10) and reactive oxygen (ROS) and nitrogen (RNS) species by resident (RMs) and thioglycolate-elicited (TGMs) peritoneal macrophages of BALB/c mice. RMs and TGMs were stimulated (48h) with ALG and DHP in concentrations previously shown to be non-cytotoxic (up to 50 and 25 μg/ml, respectively). DHP/ALG promotes simultaneous production of inflammatory (IL-6, IL-12) and regulatory cytokines by RMs in a positive dose-dependent manner. Production of inflammatory cytokines was stimulated by ALG, while an increase in IL-10 production positively correlated to the concentration of DHP. ALG also stimulated the production of IL-12 by TGMs, which was mirrored in the outcome of ALG/DHP stimulation. The significant increase in the activity of myeloperoxidase (MPO) due to DHP and/or ALG stimulation was recorded in TGMs, while a slight increase in MPO activity in RMs was recorded only upon stimulation with the higher amount of ALG. ALG in a positive dose-dependent manner stimulated the production of ROS and RNS by both RMS and TGMs. In all cases, except ROS production by RMs, the impact of ALG stimulation was mirrored in the outcome of ALG/DHP stimulation. Our results suggest that DHP/ALG exerts an immunomodulatory activity that could complement already reported antimicrobial activity and warrants further investigation on the use of DHP/ALG in the treatment of infectious diseases.",
publisher = "Institute for Biological Research "Siniša Stanković"",
journal = "Immunology at the confluence of multidisciplinary approaches",
title = "Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate",
pages = "129"
}

Kovačević, A., Lukić, I., Marinković, E., Miljković, R., Inic-Kanada, A., Spasojević, D., Radotić, K.,& Stojanović, M. M.. (2019). Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate. in Immunology at the confluence of multidisciplinary approaches
Institute for Biological Research "Siniša Stanković"., 129.

Kovačević A, Lukić I, Marinković E, Miljković R, Inic-Kanada A, Spasojević D, Radotić K, Stojanović MM. Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate. in Immunology at the confluence of multidisciplinary approaches. 2019;:129..

Kovačević, Ana, Lukić, Ivana, Marinković, Emilija, Miljković, Radmila, Inic-Kanada, Aleksandra, Spasojević, Dragica, Radotić, Ksenija, Stojanović, Marijana M., "Modulation of functional characteristics of murine peritoneal macrophages by dehydrogenate polymer from coniferyl alcohol and alginate" in Immunology at the confluence of multidisciplinary approaches (2019):129.

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Glamoclija, Jasmina
AU  - Nikolić, Milos
AU  - Soković, Marina
AU  - Milošević, Verica
AU  - Jarić, Ivana
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Barisani-Asenbauer, Talin
AU  - Prodanović, Radivoje
AU  - Jovanović, Miloš
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/467
AB  - Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP-Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - International Journal of Antimicrobial Agents
T1  - Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment
EP  - 735
IS  - 6
SP  - 732
VL  - 48
DO  - 10.1016/j.ijantimicag.2016.08.014
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Glamoclija, Jasmina and Nikolić, Milos and Soković, Marina and Milošević, Verica and Jarić, Ivana and Stojanović, Marijana and Marinković, Emilija and Barisani-Asenbauer, Talin and Prodanović, Radivoje and Jovanović, Miloš and Radotić, Ksenija",
year = "2016",
abstract = "Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP-Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Antimicrobial Agents",
title = "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment",
pages = "735-732",
number = "6",
volume = "48",
doi = "10.1016/j.ijantimicag.2016.08.014"
}

Spasojević, D., Zmejkoski, D., Glamoclija, J., Nikolić, M., Soković, M., Milošević, V., Jarić, I., Stojanović, M., Marinković, E., Barisani-Asenbauer, T., Prodanović, R., Jovanović, M.,& Radotić, K.. (2016). Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. in International Journal of Antimicrobial Agents
Elsevier, Amsterdam., 48(6), 732-735.
https://doi.org/10.1016/j.ijantimicag.2016.08.014

Spasojević D, Zmejkoski D, Glamoclija J, Nikolić M, Soković M, Milošević V, Jarić I, Stojanović M, Marinković E, Barisani-Asenbauer T, Prodanović R, Jovanović M, Radotić K. Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. in International Journal of Antimicrobial Agents. 2016;48(6):732-735.
doi:10.1016/j.ijantimicag.2016.08.014 .

Spasojević, Dragica, Zmejkoski, Danica, Glamoclija, Jasmina, Nikolić, Milos, Soković, Marina, Milošević, Verica, Jarić, Ivana, Stojanović, Marijana, Marinković, Emilija, Barisani-Asenbauer, Talin, Prodanović, Radivoje, Jovanović, Miloš, Radotić, Ksenija, "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment" in International Journal of Antimicrobial Agents, 48, no. 6 (2016):732-735,
https://doi.org/10.1016/j.ijantimicag.2016.08.014 . .