TY  - DATA
AU  - Kosanović, Dejana
AU  - Dyas, Maria
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/638
AB  - Table S1. Bacterial species used in this study with amplicon deposition number obtained from NCBI GeneBank; Figure S1. 16S rRNA gene analysis; Figure S2. Specific protein analysis- gyrB gene analysis; Figure S3a. Zone of inhibition test on NA plates104 T. aggressivum conidia per plate vs 10 μl of bacterial overnight culture incubated on 30°C; Figure S3b. Zone of inhibition test on YMEA and NA plates 104 T. aggressivum conidia per plate vs 10 μl of bacterial overnight culture incubated on 25°C; Figure S4. The effect of 25%v/v bacterial supernatants on T. aggressivum growth was assessed. B. subtilis R8.3 supernatant was found to inhibit growth of T. aggressivum by 37% and that was the maximum effect compared to other bacterial supernatants; Figure S5. PCA analysis. □ - Control group and ∆ - T. aggresivum + B. velezensis SN group; Figure S6. Protein abundance similarities of two sample group (T. aggressivum control and T. aggressivum treated with B. velezensis SN) based on hierarchical clustering; Figure S7; Figure S8. PCA analysis. □ - Control group and ∆ - A. bisporus + B. velezensis SN group.
PB  - Springer
T2  - European Journal of Plant Pathology
T1  - Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5.
IS  - 2
VL  - 160
VL  - 397
UR  - https://hdl.handle.net/21.15107/rcub_intor_638
ER  - 

@misc{
author = "Kosanović, Dejana and Dyas, Maria and Grogan, Helen and Kavanagh, Kevin",
year = "2021",
abstract = "Table S1. Bacterial species used in this study with amplicon deposition number obtained from NCBI GeneBank; Figure S1. 16S rRNA gene analysis; Figure S2. Specific protein analysis- gyrB gene analysis; Figure S3a. Zone of inhibition test on NA plates104 T. aggressivum conidia per plate vs 10 μl of bacterial overnight culture incubated on 30°C; Figure S3b. Zone of inhibition test on YMEA and NA plates 104 T. aggressivum conidia per plate vs 10 μl of bacterial overnight culture incubated on 25°C; Figure S4. The effect of 25%v/v bacterial supernatants on T. aggressivum growth was assessed. B. subtilis R8.3 supernatant was found to inhibit growth of T. aggressivum by 37% and that was the maximum effect compared to other bacterial supernatants; Figure S5. PCA analysis. □ - Control group and ∆ - T. aggresivum + B. velezensis SN group; Figure S6. Protein abundance similarities of two sample group (T. aggressivum control and T. aggressivum treated with B. velezensis SN) based on hierarchical clustering; Figure S7; Figure S8. PCA analysis. □ - Control group and ∆ - A. bisporus + B. velezensis SN group.",
publisher = "Springer",
journal = "European Journal of Plant Pathology",
title = "Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5.",
number = "2",
volume = "160, 397",
url = "https://hdl.handle.net/21.15107/rcub_intor_638"
}

Kosanović, D., Dyas, M., Grogan, H.,& Kavanagh, K.. (2021). Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5.. in European Journal of Plant Pathology
Springer., 160(2).
https://hdl.handle.net/21.15107/rcub_intor_638

Kosanović D, Dyas M, Grogan H, Kavanagh K. Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5.. in European Journal of Plant Pathology. 2021;160(2).
https://hdl.handle.net/21.15107/rcub_intor_638 .

Kosanović, Dejana, Dyas, Maria, Grogan, Helen, Kavanagh, Kevin, "Supplementary information for the article: Kosanović, D.; Dyas, M.; Grogan, H.; Kavanagh, K. Differential Proteomic Response of Agaricus Bisporus and Trichoderma Aggressivum f. Europaeum to Bacillus Velezensis Supernatant. European Journal of Plant Pathology 2021, 160 (2). https://doi.org/10.1007/s10658-021-02252-5." in European Journal of Plant Pathology, 160, no. 2 (2021),
https://hdl.handle.net/21.15107/rcub_intor_638 .

TY  - JOUR
AU  - Kosanović, Dejana
AU  - Dyas, Maria
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/613
AB  - Trichoderma aggressivum, a mycopathogen causing green mould disease, is a major problem in Agaricus bisporus cultivation due to crop loss, and resistance to chemical fungicides. There is an urgent need for novel biological ways to control mycopathogens without affecting the growth of A. bisporus. Bacteria from the mushroom-casing environment were identified and tested for antagonistic effect on T. aggressivum. Bacillus velezensis produced a large zone of inhibition and its supernatant inhibited the growth of T. aggressivum [−37%], and slightly stimulated A. bisporus growth [+2%]. Label free quantitative-proteomic (LFQ) analysis of changes in the abundance of T. aggressivum proteins following exposure to B. velezensis supernatant indicated increased abundance of proteins associated with catabolic processing of amino acids (40-fold), amino oxidase proteins (14-fold), oxidoreductase proteins (13-fold, 4-fold) and hydrolases (3-fold). Proteins that decreased in relative abundance were antioxidants (29-fold), NTF2 domain containing protein (17-fold), 60S ribosomal protein L-13 (14-fold), glucoamylase proteins (13-fold), proteasome subunit proteins (11-fold) and other ribosomal proteins (9-fold). LFQ analysis revealed that exposing A. bisporus to B. velezensis supernatant led to a decrease in: prohibitin (13-fold, 6-fold), proteasomal proteins (11-fold), cytosolic adaptor domain containing protein (5-fold), aldehyde dehydrogenase (4-fold), ribosomal proteins (4-fold), DLH domain-containing protein (4-fold) and PKS_ER domain containing protein (3-fold). The results indicate that A. bisporus was not under stress upon contact with B. velezensis. Whereas a detrimental effect of B. velezensis on T. aggressivum is shown by inhibition of growth and damage-preventing proteins and increased abundance of proteins associated with stress.
PB  - Springer
T2  - European Journal of Plant Pathology
T1  - Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant
IS  - 2
VL  - 160
VL  - 397
DO  - 10.1007/s10658-021-02252-5
ER  - 

@article{
author = "Kosanović, Dejana and Dyas, Maria and Grogan, Helen and Kavanagh, Kevin",
year = "2021",
abstract = "Trichoderma aggressivum, a mycopathogen causing green mould disease, is a major problem in Agaricus bisporus cultivation due to crop loss, and resistance to chemical fungicides. There is an urgent need for novel biological ways to control mycopathogens without affecting the growth of A. bisporus. Bacteria from the mushroom-casing environment were identified and tested for antagonistic effect on T. aggressivum. Bacillus velezensis produced a large zone of inhibition and its supernatant inhibited the growth of T. aggressivum [−37%], and slightly stimulated A. bisporus growth [+2%]. Label free quantitative-proteomic (LFQ) analysis of changes in the abundance of T. aggressivum proteins following exposure to B. velezensis supernatant indicated increased abundance of proteins associated with catabolic processing of amino acids (40-fold), amino oxidase proteins (14-fold), oxidoreductase proteins (13-fold, 4-fold) and hydrolases (3-fold). Proteins that decreased in relative abundance were antioxidants (29-fold), NTF2 domain containing protein (17-fold), 60S ribosomal protein L-13 (14-fold), glucoamylase proteins (13-fold), proteasome subunit proteins (11-fold) and other ribosomal proteins (9-fold). LFQ analysis revealed that exposing A. bisporus to B. velezensis supernatant led to a decrease in: prohibitin (13-fold, 6-fold), proteasomal proteins (11-fold), cytosolic adaptor domain containing protein (5-fold), aldehyde dehydrogenase (4-fold), ribosomal proteins (4-fold), DLH domain-containing protein (4-fold) and PKS_ER domain containing protein (3-fold). The results indicate that A. bisporus was not under stress upon contact with B. velezensis. Whereas a detrimental effect of B. velezensis on T. aggressivum is shown by inhibition of growth and damage-preventing proteins and increased abundance of proteins associated with stress.",
publisher = "Springer",
journal = "European Journal of Plant Pathology",
title = "Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant",
number = "2",
volume = "160, 397",
doi = "10.1007/s10658-021-02252-5"
}

Kosanović, D., Dyas, M., Grogan, H.,& Kavanagh, K.. (2021). Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant. in European Journal of Plant Pathology
Springer., 160(2).
https://doi.org/10.1007/s10658-021-02252-5

Kosanović D, Dyas M, Grogan H, Kavanagh K. Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant. in European Journal of Plant Pathology. 2021;160(2).
doi:10.1007/s10658-021-02252-5 .

Kosanović, Dejana, Dyas, Maria, Grogan, Helen, Kavanagh, Kevin, "Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant" in European Journal of Plant Pathology, 160, no. 2 (2021),
https://doi.org/10.1007/s10658-021-02252-5 . .

TY  - JOUR
AU  - Kosanović, Dejana
AU  - Sheehan, Gerard
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/559
AB  - Green mould disease is caused by Trichoderma aggressivum which colonizes mushroom compost and reduces yield. Two Pseudomonas species are associated with mushroom compost: Pseudomonas putida, which stimulates mushroom pinning, and Pseudomonas tolaasii which has a negative effect on crop production. The aim of this work was to characterize T. aggressivum – Pseudomonas interactions as these may be important factors in the development of green mould disease. P. tolaasii supernatant inhibited growth by 57% but P. putida stimulated growth of T.aggressivum by 44%. Tolaasin production was identified in P. tolaasii cultures with a peak at 96 h. Fluorescent microscopy examination of T. aggressivum hyphae revealed that exposure to P. tolaasii supernatant decreased mycelial formation while increasing the abundance of conidia. Label free proteomic analysis of changes in the abundance of T. aggressivum proteins indicated that exposure to P. tolaasii supernatant lead to an oxidative stress response and catabolic enzyme activation (mitochondrial import inner membrane translocase complex (5.7-fold), oxidoreductase (5.2-fold), glucoamylase (5.1-fold)). Exposure of T. aggressivum to P. putida supernatant lead to an increase in the abundance of proteins associated with growth and development (structural constituents of ribosome (20-fold), H/ACA ribonucleoprotein complex subunit (18-fold), DNA binding and nucleosome assembly protein (5.3-fold), and prefoldin (5-fold)). These results indicate that exposure to P. putida can stimulate the growth of T. aggressivum and this interaction may be an important factor in increasing green mould disease in mushroom crops and so reducing yield.
T2  - European Journal of Plant Pathology
T1  - Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum
EP  - 121
IS  - 1
SP  - 111
VL  - 156
DO  - 10.1007/s10658-019-01867-z
UR  - https://hdl.handle.net/21.15107/rcub_intor_559
ER  - 

@article{
author = "Kosanović, Dejana and Sheehan, Gerard and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Green mould disease is caused by Trichoderma aggressivum which colonizes mushroom compost and reduces yield. Two Pseudomonas species are associated with mushroom compost: Pseudomonas putida, which stimulates mushroom pinning, and Pseudomonas tolaasii which has a negative effect on crop production. The aim of this work was to characterize T. aggressivum – Pseudomonas interactions as these may be important factors in the development of green mould disease. P. tolaasii supernatant inhibited growth by 57% but P. putida stimulated growth of T.aggressivum by 44%. Tolaasin production was identified in P. tolaasii cultures with a peak at 96 h. Fluorescent microscopy examination of T. aggressivum hyphae revealed that exposure to P. tolaasii supernatant decreased mycelial formation while increasing the abundance of conidia. Label free proteomic analysis of changes in the abundance of T. aggressivum proteins indicated that exposure to P. tolaasii supernatant lead to an oxidative stress response and catabolic enzyme activation (mitochondrial import inner membrane translocase complex (5.7-fold), oxidoreductase (5.2-fold), glucoamylase (5.1-fold)). Exposure of T. aggressivum to P. putida supernatant lead to an increase in the abundance of proteins associated with growth and development (structural constituents of ribosome (20-fold), H/ACA ribonucleoprotein complex subunit (18-fold), DNA binding and nucleosome assembly protein (5.3-fold), and prefoldin (5-fold)). These results indicate that exposure to P. putida can stimulate the growth of T. aggressivum and this interaction may be an important factor in increasing green mould disease in mushroom crops and so reducing yield.",
journal = "European Journal of Plant Pathology",
title = "Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum",
pages = "121-111",
number = "1",
volume = "156",
doi = "10.1007/s10658-019-01867-z",
url = "https://hdl.handle.net/21.15107/rcub_intor_559"
}

Kosanović, D., Sheehan, G., Grogan, H.,& Kavanagh, K.. (2020). Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum. in European Journal of Plant Pathology, 156(1), 111-121.
https://doi.org/10.1007/s10658-019-01867-z
https://hdl.handle.net/21.15107/rcub_intor_559

Kosanović D, Sheehan G, Grogan H, Kavanagh K. Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum. in European Journal of Plant Pathology. 2020;156(1):111-121.
doi:10.1007/s10658-019-01867-z
https://hdl.handle.net/21.15107/rcub_intor_559 .

Kosanović, Dejana, Sheehan, Gerard, Grogan, Helen, Kavanagh, Kevin, "Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum" in European Journal of Plant Pathology, 156, no. 1 (2020):111-121,
https://doi.org/10.1007/s10658-019-01867-z .,
https://hdl.handle.net/21.15107/rcub_intor_559 .

TY  - DATA
AU  - Kosanović, Dejana
AU  - Sheehan, Gerard
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/641
AB  - Figure S1. Pathogenicity assay on mushroom caps. Top of the pilei were inoculated with 20 μl of Pseudomonas tolaasii (1×1013 CFU ml−1). Sterile water was used as a negative control. Brown blotch on mushroom caps, 48h after inoculation (b), no blotch on a control (a); Figure S2. Zones of Inhibition due to P. putida (a, b) and P. tolaasii (c, d) supernatants on PDA (b, d) and ME (a, c) plates inoculated with with 104 T. aggressivum conidia and wells filled with 50 µl of 96h SN, after 48h, 30 °C; Figure S3. Comparison of superimposed P. putida and P. tolaasii supernatant chromatographs. Tolaasin Rt = 17.418 min. detected only in 96- and 120-hours P. tolaasii supernatants; Figure S4. PCA analysis. ◦ - P. putida group, ∆ - P. tolaasii group and ▫- Control group; Figure S5. Gene Ontology analysis by Blast2GO software tool. Biological process, molecular function, and cellular components significantly enriched within the proteome of P. putida/tolaasii treated T. aggressivum.
T2  - European Journal of Plant Pathology
T1  - Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.
EP  - 121
IS  - 1
SP  - 111
VL  - 156
UR  - https://hdl.handle.net/21.15107/rcub_intor_641
ER  - 

@misc{
author = "Kosanović, Dejana and Sheehan, Gerard and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Figure S1. Pathogenicity assay on mushroom caps. Top of the pilei were inoculated with 20 μl of Pseudomonas tolaasii (1×1013 CFU ml−1). Sterile water was used as a negative control. Brown blotch on mushroom caps, 48h after inoculation (b), no blotch on a control (a); Figure S2. Zones of Inhibition due to P. putida (a, b) and P. tolaasii (c, d) supernatants on PDA (b, d) and ME (a, c) plates inoculated with with 104 T. aggressivum conidia and wells filled with 50 µl of 96h SN, after 48h, 30 °C; Figure S3. Comparison of superimposed P. putida and P. tolaasii supernatant chromatographs. Tolaasin Rt = 17.418 min. detected only in 96- and 120-hours P. tolaasii supernatants; Figure S4. PCA analysis. ◦ - P. putida group, ∆ - P. tolaasii group and ▫- Control group; Figure S5. Gene Ontology analysis by Blast2GO software tool. Biological process, molecular function, and cellular components significantly enriched within the proteome of P. putida/tolaasii treated T. aggressivum.",
journal = "European Journal of Plant Pathology",
title = "Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.",
pages = "121-111",
number = "1",
volume = "156",
url = "https://hdl.handle.net/21.15107/rcub_intor_641"
}

Kosanović, D., Sheehan, G., Grogan, H.,& Kavanagh, K.. (2020). Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.. in European Journal of Plant Pathology, 156(1), 111-121.
https://hdl.handle.net/21.15107/rcub_intor_641

Kosanović D, Sheehan G, Grogan H, Kavanagh K. Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.. in European Journal of Plant Pathology. 2020;156(1):111-121.
https://hdl.handle.net/21.15107/rcub_intor_641 .

Kosanović, Dejana, Sheehan, Gerard, Grogan, Helen, Kavanagh, Kevin, "Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z." in European Journal of Plant Pathology, 156, no. 1 (2020):111-121,
https://hdl.handle.net/21.15107/rcub_intor_641 .