Phylogenetic anaysis and molecular evolution of highly variable viruses: coinfections, host-pathogene interactions

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Phylogenetic anaysis and molecular evolution of highly variable viruses: coinfections, host-pathogene interactions (en)
Филогенетски приступ анализи молекуларне еволуције високо варијабилних вируса - коинфекције, интеракција вируса и домаћина (sr)
Filogenetski pristup analizi molekularne evolucije visoko varijabilnih virusa - koinfekcije, interakcija virusa i domaćina (sr_RS)
Authors

Publications

Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011

Stanojević, Maja; Ćirković, Valentina; Siljić, Marina; Gligić, Ana; Stamenković, Gorana

(Elsevier Science London, London, 2019)

TY  - JOUR
AU  - Stanojević, Maja
AU  - Ćirković, Valentina
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stamenković, Gorana
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/530
AB  - Background: Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. Methods: Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral anti-bodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. Results: PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. Conclusions: Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation. (C) 2019 Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
PB  - Elsevier Science London, London
T2  - Journal of Infection and Public Health
T1  - Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011
EP  - 649
IS  - 5
SP  - 645
VL  - 12
DO  - 10.1016/j.jiph.2019.02.021
UR  - conv_460
ER  - 
@article{
author = "Stanojević, Maja and Ćirković, Valentina and Siljić, Marina and Gligić, Ana and Stamenković, Gorana",
year = "2019",
abstract = "Background: Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. Methods: Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral anti-bodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. Results: PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. Conclusions: Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation. (C) 2019 Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).",
publisher = "Elsevier Science London, London",
journal = "Journal of Infection and Public Health",
title = "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011",
pages = "649-645",
number = "5",
volume = "12",
doi = "10.1016/j.jiph.2019.02.021",
url = "conv_460"
}
Stanojević, M., Ćirković, V., Siljić, M., Gligić, A.,& Stamenković, G.. (2019). Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011. in Journal of Infection and Public Health
Elsevier Science London, London., 12(5), 645-649.
https://doi.org/10.1016/j.jiph.2019.02.021
conv_460
Stanojević M, Ćirković V, Siljić M, Gligić A, Stamenković G. Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011. in Journal of Infection and Public Health. 2019;12(5):645-649.
doi:10.1016/j.jiph.2019.02.021
conv_460 .
Stanojević, Maja, Ćirković, Valentina, Siljić, Marina, Gligić, Ana, Stamenković, Gorana, "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011" in Journal of Infection and Public Health, 12, no. 5 (2019):645-649,
https://doi.org/10.1016/j.jiph.2019.02.021 .,
conv_460 .
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Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia

Stanojević, Maja; Nikolić, V.; Stajković, Novica; Stamenković, Gorana; Božović, Bojana; Čekanac, Radovan; Marušić, Predrag; Gligić, Ana

(Cambridge Univ Press, New York, 2015)

TY  - JOUR
AU  - Stanojević, Maja
AU  - Nikolić, V.
AU  - Stajković, Novica
AU  - Stamenković, Gorana
AU  - Božović, Bojana
AU  - Čekanac, Radovan
AU  - Marušić, Predrag
AU  - Gligić, Ana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/439
AB  - Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution of DOBV in its reservoir hosts in Serbia.
PB  - Cambridge Univ Press, New York
T2  - Epidemiology and Infection
T1  - Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia
EP  - 404
IS  - 2
SP  - 400
VL  - 143
DO  - 10.1017/S0950268814001010
UR  - conv_352
ER  - 
@article{
author = "Stanojević, Maja and Nikolić, V. and Stajković, Novica and Stamenković, Gorana and Božović, Bojana and Čekanac, Radovan and Marušić, Predrag and Gligić, Ana",
year = "2015",
abstract = "Hantaviruses are endemic in the Balkans, particularly in Serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. Here, we present genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences in wild rodents trapped in central Serbia. All the animals were pre-screened serologically by indirect immunofluorescence (IF) test and only those with a positive finding of hantaviral antigens were further tested by polymerase chain reaction. Of the total of 104 trapped animals, 20 were found to be IF positive and of those three were positive for hantaviral RNA: one Microtus arvalis for Tula virus, and one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic analysis of the obtained sequences implies putative DOBV spillover infection of A. agrarius and G. glis from Apodemus flavicollis. However, future investigations should help to identify the most common natural host and geographical distribution of DOBV in its reservoir hosts in Serbia.",
publisher = "Cambridge Univ Press, New York",
journal = "Epidemiology and Infection",
title = "Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia",
pages = "404-400",
number = "2",
volume = "143",
doi = "10.1017/S0950268814001010",
url = "conv_352"
}
Stanojević, M., Nikolić, V., Stajković, N., Stamenković, G., Božović, B., Čekanac, R., Marušić, P.,& Gligić, A.. (2015). Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia. in Epidemiology and Infection
Cambridge Univ Press, New York., 143(2), 400-404.
https://doi.org/10.1017/S0950268814001010
conv_352
Stanojević M, Nikolić V, Stajković N, Stamenković G, Božović B, Čekanac R, Marušić P, Gligić A. Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia. in Epidemiology and Infection. 2015;143(2):400-404.
doi:10.1017/S0950268814001010
conv_352 .
Stanojević, Maja, Nikolić, V., Stajković, Novica, Stamenković, Gorana, Božović, Bojana, Čekanac, Radovan, Marušić, Predrag, Gligić, Ana, "Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse (Glis glis) in central Serbia" in Epidemiology and Infection, 143, no. 2 (2015):400-404,
https://doi.org/10.1017/S0950268814001010 .,
conv_352 .
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Evidence of recombination in Tula virus strains from Serbia

Nikolić, Valentina; Stajković, Novica; Stamenković, Gorana; Čekanac, Radovan; Marušić, Predrag; Siljić, Marina; Gligić, Ana; Stanojević, Maja

(Elsevier, Amsterdam, 2014)

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Stajković, Novica
AU  - Stamenković, Gorana
AU  - Čekanac, Radovan
AU  - Marušić, Predrag
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stanojević, Maja
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/414
AB  - Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Infection Genetics and Evolution
T1  - Evidence of recombination in Tula virus strains from Serbia
EP  - 478
SP  - 472
VL  - 21
DO  - 10.1016/j.meegid.2013.08.020
UR  - conv_334
ER  - 
@article{
author = "Nikolić, Valentina and Stajković, Novica and Stamenković, Gorana and Čekanac, Radovan and Marušić, Predrag and Siljić, Marina and Gligić, Ana and Stanojević, Maja",
year = "2014",
abstract = "Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Infection Genetics and Evolution",
title = "Evidence of recombination in Tula virus strains from Serbia",
pages = "478-472",
volume = "21",
doi = "10.1016/j.meegid.2013.08.020",
url = "conv_334"
}
Nikolić, V., Stajković, N., Stamenković, G., Čekanac, R., Marušić, P., Siljić, M., Gligić, A.,& Stanojević, M.. (2014). Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution
Elsevier, Amsterdam., 21, 472-478.
https://doi.org/10.1016/j.meegid.2013.08.020
conv_334
Nikolić V, Stajković N, Stamenković G, Čekanac R, Marušić P, Siljić M, Gligić A, Stanojević M. Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution. 2014;21:472-478.
doi:10.1016/j.meegid.2013.08.020
conv_334 .
Nikolić, Valentina, Stajković, Novica, Stamenković, Gorana, Čekanac, Radovan, Marušić, Predrag, Siljić, Marina, Gligić, Ana, Stanojević, Maja, "Evidence of recombination in Tula virus strains from Serbia" in Infection Genetics and Evolution, 21 (2014):472-478,
https://doi.org/10.1016/j.meegid.2013.08.020 .,
conv_334 .
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