TY  - JOUR
AU  - Dimitrijević, Rajna
AU  - Stojanović, Marijana
AU  - Micić, M.
AU  - Dimitrijević, Ljiljana
AU  - Gavrović-Jankulović, Marija
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/354
AB  - To explore the potential usage of recombinant banana lectin as an immunostimulator it was of interest to analyze the effect it exerted in vivo in mice, upon two different routes of exposure. By Western blotting analysis of the gastric and intestinal luminal content it was found that recombinant banana lectin is stable in vivo in the mouse digestive tract, where it specifically interacts with the mucosal surfaces which can be inhibited by the addition of glucose. Its attachment to mice jejunum has been confirmed by immunofluorescence. In addition, it was able to induce systemic immunity, evidenced by specific antibody production, by oral application, a property that might be exploited for the induction of systemic immune responses. (c) 2012 Elsevier Ltd. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Functional Foods
T1  - Recombinant banana lectin as mucosal immunostimulator
EP  - 641
IS  - 3
SP  - 636
VL  - 4
DO  - 10.1016/j.jff.2012.04.003
ER  - 

@article{
author = "Dimitrijević, Rajna and Stojanović, Marijana and Micić, M. and Dimitrijević, Ljiljana and Gavrović-Jankulović, Marija",
year = "2012",
abstract = "To explore the potential usage of recombinant banana lectin as an immunostimulator it was of interest to analyze the effect it exerted in vivo in mice, upon two different routes of exposure. By Western blotting analysis of the gastric and intestinal luminal content it was found that recombinant banana lectin is stable in vivo in the mouse digestive tract, where it specifically interacts with the mucosal surfaces which can be inhibited by the addition of glucose. Its attachment to mice jejunum has been confirmed by immunofluorescence. In addition, it was able to induce systemic immunity, evidenced by specific antibody production, by oral application, a property that might be exploited for the induction of systemic immune responses. (c) 2012 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Functional Foods",
title = "Recombinant banana lectin as mucosal immunostimulator",
pages = "641-636",
number = "3",
volume = "4",
doi = "10.1016/j.jff.2012.04.003"
}

Dimitrijević, R., Stojanović, M., Micić, M., Dimitrijević, L.,& Gavrović-Jankulović, M.. (2012). Recombinant banana lectin as mucosal immunostimulator. in Journal of Functional Foods
Elsevier, Amsterdam., 4(3), 636-641.
https://doi.org/10.1016/j.jff.2012.04.003

Dimitrijević R, Stojanović M, Micić M, Dimitrijević L, Gavrović-Jankulović M. Recombinant banana lectin as mucosal immunostimulator. in Journal of Functional Foods. 2012;4(3):636-641.
doi:10.1016/j.jff.2012.04.003 .

Dimitrijević, Rajna, Stojanović, Marijana, Micić, M., Dimitrijević, Ljiljana, Gavrović-Jankulović, Marija, "Recombinant banana lectin as mucosal immunostimulator" in Journal of Functional Foods, 4, no. 3 (2012):636-641,
https://doi.org/10.1016/j.jff.2012.04.003 . .

TY  - JOUR
AU  - Rakin, Ana
AU  - Kuštrimović, Nataša
AU  - Kosec, Duško
AU  - Živković, Irena
AU  - Janković, I.
AU  - Mićić, Mileva
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/335
AB  - To investigate the differences between thymocytes development in vivo and in vitro, thymus lobe fragments from 12-weeks old male Albino Oxford rats were cultivated over a 7-days period. In the controls and cultivated thymic lobes fragments were evaluated and the viability, apoptosis and cell cycle of thymocytes, as well as the histological characteristics of thymic tissue. Additionally, we analyzed the expression of CD4, CD8 and TCRαβ on thymocytes by flow cytometry. The obtained results showed that thymus cellularity decreased during cultivated time due to expanded apoptosis, decreased proliferation and the absence of progenitors reseeding thymus. The relative proportion of thymocyte subsets in the first 24 hours of culture remained similar as in the control. However, cultivation for 3 and 7 days modulated the relative proportions between thymoctye subsets. The percentage of DP TCRαβlow increased, DP TCRαβhi subset remained unchanged, both SP TCRαβhi subsets decreased while the same mature SP phenotype dominated in culture media. These results demonstrate that cultivated thymic fragments retain the capacity for T cell development, although cultivation modulates this process.
AB  - Sa namerom da ispitamo razlike između in vivo i in vitro sazrevanja timocita gajili smo fragmente lobusa timusa poreklom od mužjaka Albino Oksford pacova, starih dvanaest nedelja, u vremenskom periodu od sedam dana. Nakon kultivacije određivani su vijabilnost, apoptoza i ćelijski ciklus timocita, kao i histološke osobine timusnog tkiva. Takođe je analizirano ispoljavanje markera diferentovanja CD4, CD8 and TCRαβ na površini timocita metodom tečne citofluorometrije. Dobijeni rezultati su ukazali da sedmodnevna kultivacija dovodi do smanjenja broja ćelija u timusu usled povećane apoptoze, smanjene proliferacije i odsustva ulaska progenitora timocita. Tokom prvih 24 sata kultivacije ne dolazi do promena u odnosima timocitnih populacija. Međutim, duže vreme kultivacije - 3 i 7 dana moduliše relativne odnose između timocitnih subpopulacija - povećava se procenat DP TCRαβlow, procenat DP TCRαβhi timocita ostaje nepromenjen, dok su procenti ćelija oba subseta SP TCRαβhi smanjeni, mada je prisustvo pomenutih SP subsetova dominantno u medijumu za kultivaciju. Navedeni rezultati pokazuju da kultivisani fragmenti timusnog tkiva zadržavaju sposobnost da podrže sazrevanje timocita u jednostruko pozitivne T ćelije, mada je diferentovanje timocita donekle modulisano kultivacijom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Rat thymocytes differentiation in adult thymus organ culture
T1  - Diferentovanje timocita u organ kulturi timusa odraslih pacova
EP  - 478
IS  - 5-6
SP  - 461
VL  - 61
DO  - 10.2298/AVB1106461R
ER  - 

@article{
author = "Rakin, Ana and Kuštrimović, Nataša and Kosec, Duško and Živković, Irena and Janković, I. and Mićić, Mileva",
year = "2011",
abstract = "To investigate the differences between thymocytes development in vivo and in vitro, thymus lobe fragments from 12-weeks old male Albino Oxford rats were cultivated over a 7-days period. In the controls and cultivated thymic lobes fragments were evaluated and the viability, apoptosis and cell cycle of thymocytes, as well as the histological characteristics of thymic tissue. Additionally, we analyzed the expression of CD4, CD8 and TCRαβ on thymocytes by flow cytometry. The obtained results showed that thymus cellularity decreased during cultivated time due to expanded apoptosis, decreased proliferation and the absence of progenitors reseeding thymus. The relative proportion of thymocyte subsets in the first 24 hours of culture remained similar as in the control. However, cultivation for 3 and 7 days modulated the relative proportions between thymoctye subsets. The percentage of DP TCRαβlow increased, DP TCRαβhi subset remained unchanged, both SP TCRαβhi subsets decreased while the same mature SP phenotype dominated in culture media. These results demonstrate that cultivated thymic fragments retain the capacity for T cell development, although cultivation modulates this process., Sa namerom da ispitamo razlike između in vivo i in vitro sazrevanja timocita gajili smo fragmente lobusa timusa poreklom od mužjaka Albino Oksford pacova, starih dvanaest nedelja, u vremenskom periodu od sedam dana. Nakon kultivacije određivani su vijabilnost, apoptoza i ćelijski ciklus timocita, kao i histološke osobine timusnog tkiva. Takođe je analizirano ispoljavanje markera diferentovanja CD4, CD8 and TCRαβ na površini timocita metodom tečne citofluorometrije. Dobijeni rezultati su ukazali da sedmodnevna kultivacija dovodi do smanjenja broja ćelija u timusu usled povećane apoptoze, smanjene proliferacije i odsustva ulaska progenitora timocita. Tokom prvih 24 sata kultivacije ne dolazi do promena u odnosima timocitnih populacija. Međutim, duže vreme kultivacije - 3 i 7 dana moduliše relativne odnose između timocitnih subpopulacija - povećava se procenat DP TCRαβlow, procenat DP TCRαβhi timocita ostaje nepromenjen, dok su procenti ćelija oba subseta SP TCRαβhi smanjeni, mada je prisustvo pomenutih SP subsetova dominantno u medijumu za kultivaciju. Navedeni rezultati pokazuju da kultivisani fragmenti timusnog tkiva zadržavaju sposobnost da podrže sazrevanje timocita u jednostruko pozitivne T ćelije, mada je diferentovanje timocita donekle modulisano kultivacijom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Rat thymocytes differentiation in adult thymus organ culture, Diferentovanje timocita u organ kulturi timusa odraslih pacova",
pages = "478-461",
number = "5-6",
volume = "61",
doi = "10.2298/AVB1106461R"
}

Rakin, A., Kuštrimović, N., Kosec, D., Živković, I., Janković, I.,& Mićić, M.. (2011). Rat thymocytes differentiation in adult thymus organ culture. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(5-6), 461-478.
https://doi.org/10.2298/AVB1106461R

Rakin A, Kuštrimović N, Kosec D, Živković I, Janković I, Mićić M. Rat thymocytes differentiation in adult thymus organ culture. in Acta veterinaria - Beograd. 2011;61(5-6):461-478.
doi:10.2298/AVB1106461R .

Rakin, Ana, Kuštrimović, Nataša, Kosec, Duško, Živković, Irena, Janković, I., Mićić, Mileva, "Rat thymocytes differentiation in adult thymus organ culture" in Acta veterinaria - Beograd, 61, no. 5-6 (2011):461-478,
https://doi.org/10.2298/AVB1106461R . .