TY  - JOUR
AU  - Stenovec, Matjaz
AU  - Milošević, Milena
AU  - Petrušić, Vladimir
AU  - Potokar, Maja
AU  - Stević, Zorica
AU  - Prebil, Mateja
AU  - Kreft, Marko
AU  - Trkov, Saša
AU  - Anđus, Pavle
AU  - Zorec, Robert
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/331
AB  - Aim: We examined the effect of purified immunoglobulins G (IgG) from patients with amyotrophic lateral sclerosis (ALS) on the mobility and exocytotic release from Lysotracker-stained vesicles in cultured rat astrocytes. Methods: Time-lapse confocal images were acquired, and vesicle mobility was analysed before and after the application of ALS IgG. The vesicle counts were obtained to assess cargo exocytosis from stained organelles. Results: At rest, when mobility was monitored for 2 min in bath with Ca2+, two vesicle populations were discovered: (1) non-mobile vesicles (6.1%) with total track length (TL)  lt  1 mu m, averaging at 0.33 +/- 0.01 mu m (n = 1305) and (2) mobile vesicles (93.9%) with TL  gt  1 mu m, averaging at 3.03 +/- 0.01 mu m (n = 20 200). ALS IgG (0.1 mg mL(-1)) from 12 of 13 patients increased the TL of mobile vesicles by approx. 24% and maximal displacement (MD) by approx. 26% within 4 min, while the IgG from control group did not alter the vesicle mobility. The mobility enhancement by ALS IgG was reduced in extracellular solution devoid of Ca2+, indicating that ALS IgG vesicle mobility enhancement involves changes in Ca2+ homeostasis. To examine whether enhanced mobility relates to elevated Ca2+ activity, cells were stimulated by 1 mm ATP, a cytosolic Ca2+ increasing agent, in the presence (2 mm) and in the absence of extracellular Ca2+. ATP stimulation triggered an increase in TL by approx. 7% and 12% and a decrease in MD by approx. 11% and 1%, within 4 min respectively. Interestingly, none of the stimuli triggered the release of vesicle cargo. Conclusion: Amyotrophic lateral sclerosis-IgG-enhanced vesicle mobility in astrocytes engages changes in calcium homeostasis.
PB  - Wiley, Hoboken
T2  - Acta Physiologica
T1  - Amyotrophic lateral sclerosis immunoglobulins G enhance the mobility of Lysotracker-labelled vesicles in cultured rat astrocytes
EP  - 471
IS  - 4
SP  - 457
VL  - 203
DO  - 10.1111/j.1748-1716.2011.02337.x
ER  - 

@article{
author = "Stenovec, Matjaz and Milošević, Milena and Petrušić, Vladimir and Potokar, Maja and Stević, Zorica and Prebil, Mateja and Kreft, Marko and Trkov, Saša and Anđus, Pavle and Zorec, Robert",
year = "2011",
abstract = "Aim: We examined the effect of purified immunoglobulins G (IgG) from patients with amyotrophic lateral sclerosis (ALS) on the mobility and exocytotic release from Lysotracker-stained vesicles in cultured rat astrocytes. Methods: Time-lapse confocal images were acquired, and vesicle mobility was analysed before and after the application of ALS IgG. The vesicle counts were obtained to assess cargo exocytosis from stained organelles. Results: At rest, when mobility was monitored for 2 min in bath with Ca2+, two vesicle populations were discovered: (1) non-mobile vesicles (6.1%) with total track length (TL)  lt  1 mu m, averaging at 0.33 +/- 0.01 mu m (n = 1305) and (2) mobile vesicles (93.9%) with TL  gt  1 mu m, averaging at 3.03 +/- 0.01 mu m (n = 20 200). ALS IgG (0.1 mg mL(-1)) from 12 of 13 patients increased the TL of mobile vesicles by approx. 24% and maximal displacement (MD) by approx. 26% within 4 min, while the IgG from control group did not alter the vesicle mobility. The mobility enhancement by ALS IgG was reduced in extracellular solution devoid of Ca2+, indicating that ALS IgG vesicle mobility enhancement involves changes in Ca2+ homeostasis. To examine whether enhanced mobility relates to elevated Ca2+ activity, cells were stimulated by 1 mm ATP, a cytosolic Ca2+ increasing agent, in the presence (2 mm) and in the absence of extracellular Ca2+. ATP stimulation triggered an increase in TL by approx. 7% and 12% and a decrease in MD by approx. 11% and 1%, within 4 min respectively. Interestingly, none of the stimuli triggered the release of vesicle cargo. Conclusion: Amyotrophic lateral sclerosis-IgG-enhanced vesicle mobility in astrocytes engages changes in calcium homeostasis.",
publisher = "Wiley, Hoboken",
journal = "Acta Physiologica",
title = "Amyotrophic lateral sclerosis immunoglobulins G enhance the mobility of Lysotracker-labelled vesicles in cultured rat astrocytes",
pages = "471-457",
number = "4",
volume = "203",
doi = "10.1111/j.1748-1716.2011.02337.x"
}

Stenovec, M., Milošević, M., Petrušić, V., Potokar, M., Stević, Z., Prebil, M., Kreft, M., Trkov, S., Anđus, P.,& Zorec, R.. (2011). Amyotrophic lateral sclerosis immunoglobulins G enhance the mobility of Lysotracker-labelled vesicles in cultured rat astrocytes. in Acta Physiologica
Wiley, Hoboken., 203(4), 457-471.
https://doi.org/10.1111/j.1748-1716.2011.02337.x

Stenovec M, Milošević M, Petrušić V, Potokar M, Stević Z, Prebil M, Kreft M, Trkov S, Anđus P, Zorec R. Amyotrophic lateral sclerosis immunoglobulins G enhance the mobility of Lysotracker-labelled vesicles in cultured rat astrocytes. in Acta Physiologica. 2011;203(4):457-471.
doi:10.1111/j.1748-1716.2011.02337.x .

Stenovec, Matjaz, Milošević, Milena, Petrušić, Vladimir, Potokar, Maja, Stević, Zorica, Prebil, Mateja, Kreft, Marko, Trkov, Saša, Anđus, Pavle, Zorec, Robert, "Amyotrophic lateral sclerosis immunoglobulins G enhance the mobility of Lysotracker-labelled vesicles in cultured rat astrocytes" in Acta Physiologica, 203, no. 4 (2011):457-471,
https://doi.org/10.1111/j.1748-1716.2011.02337.x . .