Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200015 (University of Belgrade, Institute for Medical Research)

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Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200015 (University of Belgrade, Institute for Medical Research) (en)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije, Ugovor br. 451-03-68/2020-14/200015 (Univerzitet u Beogradu, Institut za medicinska istraživanja) (sr_RS)
Министарство просвете, науке и технолошког развоја Републике Србије, Уговор бр. 451-03-68/2020-14/200015 (Универзитет у Београду, Институт за медицинска истраживања) (sr)
Authors

Publications

Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans

Nikodijevic, Slavomir; Blagojevic, Veljko; Curuvija, Ivana; Kosanovic, Dejana; Djukic, Tamara; Djordjevic, Brizita; Ilic, Vesna; Minic, Rajna

(The Scandinavian Foundation for Immunology, 2022)

TY  - JOUR
AU  - Nikodijevic, Slavomir
AU  - Blagojevic, Veljko
AU  - Curuvija, Ivana
AU  - Kosanovic, Dejana
AU  - Djukic, Tamara
AU  - Djordjevic, Brizita
AU  - Ilic, Vesna
AU  - Minic, Rajna
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/632
AB  - Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r = .70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r = .86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r = .88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.
PB  - The Scandinavian Foundation for Immunology
T2  - Scandinavian Journal of Immunology
T1  - Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans
VL  - 96
DO  - 10.1111/sji.13223
ER  - 
@article{
author = "Nikodijevic, Slavomir and Blagojevic, Veljko and Curuvija, Ivana and Kosanovic, Dejana and Djukic, Tamara and Djordjevic, Brizita and Ilic, Vesna and Minic, Rajna",
year = "2022",
abstract = "Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r = .70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r = .86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r = .88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.",
publisher = "The Scandinavian Foundation for Immunology",
journal = "Scandinavian Journal of Immunology",
title = "Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans",
volume = "96",
doi = "10.1111/sji.13223"
}
Nikodijevic, S., Blagojevic, V., Curuvija, I., Kosanovic, D., Djukic, T., Djordjevic, B., Ilic, V.,& Minic, R.. (2022). Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology
The Scandinavian Foundation for Immunology., 96.
https://doi.org/10.1111/sji.13223
Nikodijevic S, Blagojevic V, Curuvija I, Kosanovic D, Djukic T, Djordjevic B, Ilic V, Minic R. Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology. 2022;96.
doi:10.1111/sji.13223 .
Nikodijevic, Slavomir, Blagojevic, Veljko, Curuvija, Ivana, Kosanovic, Dejana, Djukic, Tamara, Djordjevic, Brizita, Ilic, Vesna, Minic, Rajna, "Selectivity of polyclonal repertoire of anti-microbial IgA and its subclasses in saliva and serum in humans" in Scandinavian Journal of Immunology, 96 (2022),
https://doi.org/10.1111/sji.13223 . .

West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera

Vasić, Ana; Răileanu, Cristian; Körsten, Christin; Vojinović, Dragica; Manić, Marija; Urošević, Aleksandar; Nikolić, Nataša; Dulović, Olga; Tews, Birke Andrea; Petrović, Tamaš; Silaghi, Cornelia; Valčić, Miroslav; Gligić, Ana

(2022)

TY  - JOUR
AU  - Vasić, Ana
AU  - Răileanu, Cristian
AU  - Körsten, Christin
AU  - Vojinović, Dragica
AU  - Manić, Marija
AU  - Urošević, Aleksandar
AU  - Nikolić, Nataša
AU  - Dulović, Olga
AU  - Tews, Birke Andrea
AU  - Petrović, Tamaš
AU  - Silaghi, Cornelia
AU  - Valčić, Miroslav
AU  - Gligić, Ana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1238
UR  - http://intor.torlakinstitut.com/handle/123456789/623
AB  - West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.
T2  - Transboundary and Emerging Diseases
T1  - West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera
EP  - e2515
IS  - 5
SP  - e2506
VL  - 69
DO  - 10.1111/tbed.14593
ER  - 
@article{
author = "Vasić, Ana and Răileanu, Cristian and Körsten, Christin and Vojinović, Dragica and Manić, Marija and Urošević, Aleksandar and Nikolić, Nataša and Dulović, Olga and Tews, Birke Andrea and Petrović, Tamaš and Silaghi, Cornelia and Valčić, Miroslav and Gligić, Ana",
year = "2022",
abstract = "West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.",
journal = "Transboundary and Emerging Diseases",
title = "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera",
pages = "e2515-e2506",
number = "5",
volume = "69",
doi = "10.1111/tbed.14593"
}
Vasić, A., Răileanu, C., Körsten, C., Vojinović, D., Manić, M., Urošević, A., Nikolić, N., Dulović, O., Tews, B. A., Petrović, T., Silaghi, C., Valčić, M.,& Gligić, A.. (2022). West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases, 69(5), e2506-e2515.
https://doi.org/10.1111/tbed.14593
Vasić A, Răileanu C, Körsten C, Vojinović D, Manić M, Urošević A, Nikolić N, Dulović O, Tews BA, Petrović T, Silaghi C, Valčić M, Gligić A. West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases. 2022;69(5):e2506-e2515.
doi:10.1111/tbed.14593 .
Vasić, Ana, Răileanu, Cristian, Körsten, Christin, Vojinović, Dragica, Manić, Marija, Urošević, Aleksandar, Nikolić, Nataša, Dulović, Olga, Tews, Birke Andrea, Petrović, Tamaš, Silaghi, Cornelia, Valčić, Miroslav, Gligić, Ana, "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera" in Transboundary and Emerging Diseases, 69, no. 5 (2022):e2506-e2515,
https://doi.org/10.1111/tbed.14593 . .