TY  - JOUR
AU  - Kosanović, Dejana
AU  - Potočnik, Ivana
AU  - Vukojević, Jelena
AU  - Stajić, Mirjana
AU  - Rekanović, Emil
AU  - Stepanović, Milos
AU  - Todorović, Biljana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/441
AB  - Trichoderma species, the causal agents of green mould disease, induce great losses in Agaricus bisporus farms. Fungicides are widely used to control mushroom diseases although green mould control is encumbered with difficulties. The aims of this study were, therefore, to research in vitro toxicity of several commercial fungicides to Trichoderma isolates originating from Serbian and Bosnia-Herzegovina farms, and to evaluate the effects of pH and light on their growth. The majority of isolates demonstrated optimal growth at pH 5.0, and the rest at pH 6.0. A few isolates also grew well at pH 7. The weakest mycelial growth was noted at pH 8.0-9.0. Generally, light had an inhibitory effect on the growth of tested isolates. The isolates showed the highest susceptibility to chlorothalonil and carbendazim (ED50 less than 1mg L-1), and were less sensitive to iprodione (ED50 ranged 0.84-6.72mg L-1), weakly resistant to thiophanate-methyl (ED50 = 3.75-24.13mg L-1), and resistant to trifloxystrobin (ED50 = 10.25-178.23mg L-1). Considering the toxicity of fungicides to A. bisporus, carbendazim showed the best selective toxicity (0.02), iprodione and chlorothalonil moderate (0.16), and thiophanate-methyl the lowest (1.24), while trifloxystrobin toxicity to A. bisporus was not tested because of its inefficiency against Trichoderma isolates.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was
T1  - Fungicide sensitivity of Trichoderma spp. from Agaricus bisporus farms in Serbia
EP  - 613
IS  - 8
SP  - 607
VL  - 50
DO  - 10.1080/03601234.2015.1028849
ER  - 

@article{
author = "Kosanović, Dejana and Potočnik, Ivana and Vukojević, Jelena and Stajić, Mirjana and Rekanović, Emil and Stepanović, Milos and Todorović, Biljana",
year = "2015",
abstract = "Trichoderma species, the causal agents of green mould disease, induce great losses in Agaricus bisporus farms. Fungicides are widely used to control mushroom diseases although green mould control is encumbered with difficulties. The aims of this study were, therefore, to research in vitro toxicity of several commercial fungicides to Trichoderma isolates originating from Serbian and Bosnia-Herzegovina farms, and to evaluate the effects of pH and light on their growth. The majority of isolates demonstrated optimal growth at pH 5.0, and the rest at pH 6.0. A few isolates also grew well at pH 7. The weakest mycelial growth was noted at pH 8.0-9.0. Generally, light had an inhibitory effect on the growth of tested isolates. The isolates showed the highest susceptibility to chlorothalonil and carbendazim (ED50 less than 1mg L-1), and were less sensitive to iprodione (ED50 ranged 0.84-6.72mg L-1), weakly resistant to thiophanate-methyl (ED50 = 3.75-24.13mg L-1), and resistant to trifloxystrobin (ED50 = 10.25-178.23mg L-1). Considering the toxicity of fungicides to A. bisporus, carbendazim showed the best selective toxicity (0.02), iprodione and chlorothalonil moderate (0.16), and thiophanate-methyl the lowest (1.24), while trifloxystrobin toxicity to A. bisporus was not tested because of its inefficiency against Trichoderma isolates.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was",
title = "Fungicide sensitivity of Trichoderma spp. from Agaricus bisporus farms in Serbia",
pages = "613-607",
number = "8",
volume = "50",
doi = "10.1080/03601234.2015.1028849"
}

Kosanović, D., Potočnik, I., Vukojević, J., Stajić, M., Rekanović, E., Stepanović, M.,& Todorović, B.. (2015). Fungicide sensitivity of Trichoderma spp. from Agaricus bisporus farms in Serbia. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was
Taylor & Francis Inc, Philadelphia., 50(8), 607-613.
https://doi.org/10.1080/03601234.2015.1028849

Kosanović D, Potočnik I, Vukojević J, Stajić M, Rekanović E, Stepanović M, Todorović B. Fungicide sensitivity of Trichoderma spp. from Agaricus bisporus farms in Serbia. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was. 2015;50(8):607-613.
doi:10.1080/03601234.2015.1028849 .

Kosanović, Dejana, Potočnik, Ivana, Vukojević, Jelena, Stajić, Mirjana, Rekanović, Emil, Stepanović, Milos, Todorović, Biljana, "Fungicide sensitivity of Trichoderma spp. from Agaricus bisporus farms in Serbia" in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was, 50, no. 8 (2015):607-613,
https://doi.org/10.1080/03601234.2015.1028849 . .

TY  - JOUR
AU  - Kosanović, Dejana
AU  - Potočnik, Ivana
AU  - Duduk, Bojan
AU  - Vukojević, Jelena
AU  - Stajić, Mirjana
AU  - Rekanović, Emil
AU  - Milijašević-Marčić, Svetlana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/384
AB  - Twenty Trichoderma isolates were collected on 13 Serbian Agaricus bisporus farms and one in Bosnia and Herzegovina during 2006–2010. Twelve isolates were classified into five species by standard mycological studies and ITS1/ITS4 sequence analyses, namely Trichoderma atroviride, Trichoderma koningii, Trichoderma virens, Trichoderma aggressivum f. europaeum and Trichoderma harzianum. Eight isolates were not identified to the species level but were shown to be related to T. harzianum. The isolates of T. harzianum exhibited the highest virulence to the harvested A. bisporus pilei and T. virens and T. aggressivum f. europaeum the lowest. Antifungal activity of two biofungicides based on Bacillus subtilis and tea tree oil and the fungicide prochloraz manganese were tested in vitro to all Trichoderma isolates. Prochloraz manganese and B. subtilis were highly toxic to all tested Trichoderma isolates, their ED50 values were below 0.3 and 1.3 mg L−1, respectively. Tea tree oil did not exhibit a significant antifungal activity (ED50 = 11.9–370.8 mg L−1). The effectiveness of biofungicides was evaluated against T. harzianum in a mushroom growing room, and they were applied alone or in combination with the fungicide at a respective proportion of 20:80%. Prochloraz manganese showed higher effectiveness than both tested biofungicides or their respective mixtures. The biofungicide based on B. subtilis demonstrated greater effectiveness in preventing disease symptoms than tea tree oil. B. subtilis combined with the fungicide revealed less antagonism in effectiveness against pathogen than tea tree oil.
T2  - Ann Appl Biol
T1  - Trichoderma species on Agaricus bisporus farms in Serbia and their biocontrol
EP  - 230
SP  - 218
VL  - 63
DO  - 10.1111/aab.12048
UR  - https://hdl.handle.net/21.15107/rcub_intor_384
ER  - 

@article{
author = "Kosanović, Dejana and Potočnik, Ivana and Duduk, Bojan and Vukojević, Jelena and Stajić, Mirjana and Rekanović, Emil and Milijašević-Marčić, Svetlana",
year = "2013",
abstract = "Twenty Trichoderma isolates were collected on 13 Serbian Agaricus bisporus farms and one in Bosnia and Herzegovina during 2006–2010. Twelve isolates were classified into five species by standard mycological studies and ITS1/ITS4 sequence analyses, namely Trichoderma atroviride, Trichoderma koningii, Trichoderma virens, Trichoderma aggressivum f. europaeum and Trichoderma harzianum. Eight isolates were not identified to the species level but were shown to be related to T. harzianum. The isolates of T. harzianum exhibited the highest virulence to the harvested A. bisporus pilei and T. virens and T. aggressivum f. europaeum the lowest. Antifungal activity of two biofungicides based on Bacillus subtilis and tea tree oil and the fungicide prochloraz manganese were tested in vitro to all Trichoderma isolates. Prochloraz manganese and B. subtilis were highly toxic to all tested Trichoderma isolates, their ED50 values were below 0.3 and 1.3 mg L−1, respectively. Tea tree oil did not exhibit a significant antifungal activity (ED50 = 11.9–370.8 mg L−1). The effectiveness of biofungicides was evaluated against T. harzianum in a mushroom growing room, and they were applied alone or in combination with the fungicide at a respective proportion of 20:80%. Prochloraz manganese showed higher effectiveness than both tested biofungicides or their respective mixtures. The biofungicide based on B. subtilis demonstrated greater effectiveness in preventing disease symptoms than tea tree oil. B. subtilis combined with the fungicide revealed less antagonism in effectiveness against pathogen than tea tree oil.",
journal = "Ann Appl Biol",
title = "Trichoderma species on Agaricus bisporus farms in Serbia and their biocontrol",
pages = "230-218",
volume = "63",
doi = "10.1111/aab.12048",
url = "https://hdl.handle.net/21.15107/rcub_intor_384"
}

Kosanović, D., Potočnik, I., Duduk, B., Vukojević, J., Stajić, M., Rekanović, E.,& Milijašević-Marčić, S.. (2013). Trichoderma species on Agaricus bisporus farms in Serbia and their biocontrol. in Ann Appl Biol, 63, 218-230.
https://doi.org/10.1111/aab.12048
https://hdl.handle.net/21.15107/rcub_intor_384

Kosanović D, Potočnik I, Duduk B, Vukojević J, Stajić M, Rekanović E, Milijašević-Marčić S. Trichoderma species on Agaricus bisporus farms in Serbia and their biocontrol. in Ann Appl Biol. 2013;63:218-230.
doi:10.1111/aab.12048
https://hdl.handle.net/21.15107/rcub_intor_384 .

Kosanović, Dejana, Potočnik, Ivana, Duduk, Bojan, Vukojević, Jelena, Stajić, Mirjana, Rekanović, Emil, Milijašević-Marčić, Svetlana, "Trichoderma species on Agaricus bisporus farms in Serbia and their biocontrol" in Ann Appl Biol, 63 (2013):218-230,
https://doi.org/10.1111/aab.12048 .,
https://hdl.handle.net/21.15107/rcub_intor_384 .