TY  - CONF
AU  - Stanković, Ivan
AU  - Michalickova, Danica
AU  - Minić, Rajna
AU  - Kotur-Stevuljević, Jelena
AU  - Miljković, Milica
AU  - Kostić-Vučićević, Marija
AU  - Dikić, Nenad
AU  - Anđelković, Marija
AU  - Baralić, Ivana
AU  - Ivanović, Nevena
AU  - Đorđević, Brižita
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/481
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Medicine and Science in Sports and Exercise
T1  - Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter
EP  - 457
IS  - 5
SP  - 457
VL  - 49
DO  - 10.1249/01.mss.0000518140.98604.f5
ER  - 

@conference{
author = "Stanković, Ivan and Michalickova, Danica and Minić, Rajna and Kotur-Stevuljević, Jelena and Miljković, Milica and Kostić-Vučićević, Marija and Dikić, Nenad and Anđelković, Marija and Baralić, Ivana and Ivanović, Nevena and Đorđević, Brižita",
year = "2017",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Medicine and Science in Sports and Exercise",
title = "Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter",
pages = "457-457",
number = "5",
volume = "49",
doi = "10.1249/01.mss.0000518140.98604.f5"
}

Stanković, I., Michalickova, D., Minić, R., Kotur-Stevuljević, J., Miljković, M., Kostić-Vučićević, M., Dikić, N., Anđelković, M., Baralić, I., Ivanović, N.,& Đorđević, B.. (2017). Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter. in Medicine and Science in Sports and Exercise
Lippincott Williams & Wilkins, Philadelphia., 49(5), 457-457.
https://doi.org/10.1249/01.mss.0000518140.98604.f5

Stanković I, Michalickova D, Minić R, Kotur-Stevuljević J, Miljković M, Kostić-Vučićević M, Dikić N, Anđelković M, Baralić I, Ivanović N, Đorđević B. Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter. in Medicine and Science in Sports and Exercise. 2017;49(5):457-457.
doi:10.1249/01.mss.0000518140.98604.f5 .

Stanković, Ivan, Michalickova, Danica, Minić, Rajna, Kotur-Stevuljević, Jelena, Miljković, Milica, Kostić-Vučićević, Marija, Dikić, Nenad, Anđelković, Marija, Baralić, Ivana, Ivanović, Nevena, Đorđević, Brižita, "Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter" in Medicine and Science in Sports and Exercise, 49, no. 5 (2017):457-457,
https://doi.org/10.1249/01.mss.0000518140.98604.f5 . .

TY  - CONF
AU  - Minić, Rajna
AU  - Ivanović, Nevena
AU  - Živković, Irena
AU  - Rajnpreht, Irena
AU  - Đorđević, Brižita
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2636
UR  - http://intor.torlakinstitut.com/handle/123456789/840
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Clinical Gastroenterology
T1  - Analysis of the Durability of the Beneficial Effects of Lactobacilli on Mice Fed High Fat Diet
EP  - S227
IS  - Supplement 2
SP  - S227
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_intor_840
ER  - 

@conference{
author = "Minić, Rajna and Ivanović, Nevena and Živković, Irena and Rajnpreht, Irena and Đorđević, Brižita",
year = "2016",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Clinical Gastroenterology",
title = "Analysis of the Durability of the Beneficial Effects of Lactobacilli on Mice Fed High Fat Diet",
pages = "S227-S227",
number = "Supplement 2",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_intor_840"
}

Minić, R., Ivanović, N., Živković, I., Rajnpreht, I.,& Đorđević, B.. (2016). Analysis of the Durability of the Beneficial Effects of Lactobacilli on Mice Fed High Fat Diet. in Journal of Clinical Gastroenterology
Lippincott Williams & Wilkins, Philadelphia., 50(Supplement 2), S227-S227.
https://hdl.handle.net/21.15107/rcub_intor_840

Minić R, Ivanović N, Živković I, Rajnpreht I, Đorđević B. Analysis of the Durability of the Beneficial Effects of Lactobacilli on Mice Fed High Fat Diet. in Journal of Clinical Gastroenterology. 2016;50(Supplement 2):S227-S227.
https://hdl.handle.net/21.15107/rcub_intor_840 .

Minić, Rajna, Ivanović, Nevena, Živković, Irena, Rajnpreht, Irena, Đorđević, Brižita, "Analysis of the Durability of the Beneficial Effects of Lactobacilli on Mice Fed High Fat Diet" in Journal of Clinical Gastroenterology, 50, no. Supplement 2 (2016):S227-S227,
https://hdl.handle.net/21.15107/rcub_intor_840 .

TY  - JOUR
AU  - Ivanović, Nevena
AU  - Minić, Rajna
AU  - Đuričić, Ivana
AU  - Radojević-Skodrić, Sanja
AU  - Živković, Irena
AU  - Šobajić, Slađana
AU  - Đorđević, Brižita
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/472
AB  - Western life style, and high calorie diet in particular is causing major health problems such as insulin resistance, hepatic steatosis and heart disease in the modern age. High fat diet (HFD) induces similar changes in mice, such as increased body weight, hypercholesterolemia and accumulation of triglycerides in the liver. These changes can be ameliorated by the administration of some Lactobacillus species. The focus of this study was to analyze the fatty acid content of liver, heart and brain tissues of mice fed HFD and administered with either Lactobacillus plantarum WCFS1 or Lactobacillus rhamnosus LA68, and to analyze the fatty acid content of these organs after a two months washout period. The fatty acid composition of mouse liver tissue changed significantly due to probiotic administration during a 12 weeks HFD regime and active Lactobacillus administration had a slightly reversing effect toward the standard mouse diet group, but after the washout period these changes disappeared. The fatty acid composition of the heart and brain tissues was significantly changed in the HFD regime but probiotic administration had no significant influence on the fatty acid profile of these two organs. Upon the 8 weeks washout period the only remaining beneficial effect was the significantly lower mouse weight in the supplemented groups compared to the HFD group.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime
EP  - 2848
IS  - 6
SP  - 2840
VL  - 7
DO  - 10.1039/c5fo01432h
ER  - 

@article{
author = "Ivanović, Nevena and Minić, Rajna and Đuričić, Ivana and Radojević-Skodrić, Sanja and Živković, Irena and Šobajić, Slađana and Đorđević, Brižita",
year = "2016",
abstract = "Western life style, and high calorie diet in particular is causing major health problems such as insulin resistance, hepatic steatosis and heart disease in the modern age. High fat diet (HFD) induces similar changes in mice, such as increased body weight, hypercholesterolemia and accumulation of triglycerides in the liver. These changes can be ameliorated by the administration of some Lactobacillus species. The focus of this study was to analyze the fatty acid content of liver, heart and brain tissues of mice fed HFD and administered with either Lactobacillus plantarum WCFS1 or Lactobacillus rhamnosus LA68, and to analyze the fatty acid content of these organs after a two months washout period. The fatty acid composition of mouse liver tissue changed significantly due to probiotic administration during a 12 weeks HFD regime and active Lactobacillus administration had a slightly reversing effect toward the standard mouse diet group, but after the washout period these changes disappeared. The fatty acid composition of the heart and brain tissues was significantly changed in the HFD regime but probiotic administration had no significant influence on the fatty acid profile of these two organs. Upon the 8 weeks washout period the only remaining beneficial effect was the significantly lower mouse weight in the supplemented groups compared to the HFD group.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime",
pages = "2848-2840",
number = "6",
volume = "7",
doi = "10.1039/c5fo01432h"
}

Ivanović, N., Minić, R., Đuričić, I., Radojević-Skodrić, S., Živković, I., Šobajić, S.,& Đorđević, B.. (2016). Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime. in Food & Function
Royal Soc Chemistry, Cambridge., 7(6), 2840-2848.
https://doi.org/10.1039/c5fo01432h

Ivanović N, Minić R, Đuričić I, Radojević-Skodrić S, Živković I, Šobajić S, Đorđević B. Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime. in Food & Function. 2016;7(6):2840-2848.
doi:10.1039/c5fo01432h .

Ivanović, Nevena, Minić, Rajna, Đuričić, Ivana, Radojević-Skodrić, Sanja, Živković, Irena, Šobajić, Slađana, Đorđević, Brižita, "Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime" in Food & Function, 7, no. 6 (2016):2840-2848,
https://doi.org/10.1039/c5fo01432h . .

TY  - CONF
AU  - Đorđević, Brižita
AU  - Marinković, Danica
AU  - Minić, Rajna
AU  - Ivanović, Nevena
AU  - Dikić, Nenad
AU  - Anđelković, Marija
AU  - Živković, Irena
AU  - Stanković, Ivan
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/452
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Medicine and Science in Sports and Exercise
T1  - Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes
EP  - 246
IS  - 5
SP  - 246
VL  - 48
DO  - 10.1249/01.mss.0000485740.06462.11
ER  - 

@conference{
author = "Đorđević, Brižita and Marinković, Danica and Minić, Rajna and Ivanović, Nevena and Dikić, Nenad and Anđelković, Marija and Živković, Irena and Stanković, Ivan",
year = "2016",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Medicine and Science in Sports and Exercise",
title = "Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes",
pages = "246-246",
number = "5",
volume = "48",
doi = "10.1249/01.mss.0000485740.06462.11"
}

Đorđević, B., Marinković, D., Minić, R., Ivanović, N., Dikić, N., Anđelković, M., Živković, I.,& Stanković, I.. (2016). Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes. in Medicine and Science in Sports and Exercise
Lippincott Williams & Wilkins, Philadelphia., 48(5), 246-246.
https://doi.org/10.1249/01.mss.0000485740.06462.11

Đorđević B, Marinković D, Minić R, Ivanović N, Dikić N, Anđelković M, Živković I, Stanković I. Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes. in Medicine and Science in Sports and Exercise. 2016;48(5):246-246.
doi:10.1249/01.mss.0000485740.06462.11 .

Đorđević, Brižita, Marinković, Danica, Minić, Rajna, Ivanović, Nevena, Dikić, Nenad, Anđelković, Marija, Živković, Irena, Stanković, Ivan, "Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes" in Medicine and Science in Sports and Exercise, 48, no. 5 (2016):246-246,
https://doi.org/10.1249/01.mss.0000485740.06462.11 . .

TY  - JOUR
AU  - Ivanović, Nevena
AU  - Minić, Rajna
AU  - Dimitrijević, Ljiljana
AU  - Radojević-Skodrić, Sanja
AU  - Živković, Irena
AU  - Đorđević, Brižita
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/445
AB  - In this study, two Lactobacillus strains (L. rhamnosus LA68 and L. plantarum WCFS1) were evaluated for their effects on high fat diet induced pathology in mice. The aim was to determine whether the administration of lactic acid bacteria had beneficial effects on ameliorating pathology. C57BL/6 mice fed a high fat diet were orally administered with the Lactobacillus strains. Both the metabolic and immunological parameters were analyzed. The administration of both of the strains had beneficial effects on mouse weight, serum cholesterol, TNF-alpha levels and liver histology. LA68 lowered the total cholesterol and HDL levels more prominently, whereas WCFS1 was more potent in lowering the TG and LDL levels. Leptin and adiponectin levels were increased in all experimental groups to different extents. The administration of L. plantarum WCFS1 led to a marked increase in leptin levels, as well as an increase in CD3+CD4+ and CD3+CD8+ cells, and a decrease of CD25+ cells, and had a lowering effect on IL-6 production and cell metabolic activity. In conclusion, active administration of both Lactobacillus strains had a positive effect on HFD-induced pathology. Although both of the tested strains had beneficial effects, oral administration of WCFS1 increased leptin levels and had a more prominent immunomodulatory effect, which should be taken into consideration in case of humane usage.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis
EP  - 565
IS  - 2
SP  - 558
VL  - 6
DO  - 10.1039/c4fo00843j
ER  - 

@article{
author = "Ivanović, Nevena and Minić, Rajna and Dimitrijević, Ljiljana and Radojević-Skodrić, Sanja and Živković, Irena and Đorđević, Brižita",
year = "2015",
abstract = "In this study, two Lactobacillus strains (L. rhamnosus LA68 and L. plantarum WCFS1) were evaluated for their effects on high fat diet induced pathology in mice. The aim was to determine whether the administration of lactic acid bacteria had beneficial effects on ameliorating pathology. C57BL/6 mice fed a high fat diet were orally administered with the Lactobacillus strains. Both the metabolic and immunological parameters were analyzed. The administration of both of the strains had beneficial effects on mouse weight, serum cholesterol, TNF-alpha levels and liver histology. LA68 lowered the total cholesterol and HDL levels more prominently, whereas WCFS1 was more potent in lowering the TG and LDL levels. Leptin and adiponectin levels were increased in all experimental groups to different extents. The administration of L. plantarum WCFS1 led to a marked increase in leptin levels, as well as an increase in CD3+CD4+ and CD3+CD8+ cells, and a decrease of CD25+ cells, and had a lowering effect on IL-6 production and cell metabolic activity. In conclusion, active administration of both Lactobacillus strains had a positive effect on HFD-induced pathology. Although both of the tested strains had beneficial effects, oral administration of WCFS1 increased leptin levels and had a more prominent immunomodulatory effect, which should be taken into consideration in case of humane usage.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis",
pages = "565-558",
number = "2",
volume = "6",
doi = "10.1039/c4fo00843j"
}

Ivanović, N., Minić, R., Dimitrijević, L., Radojević-Skodrić, S., Živković, I.,& Đorđević, B.. (2015). Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis. in Food & Function
Royal Soc Chemistry, Cambridge., 6(2), 558-565.
https://doi.org/10.1039/c4fo00843j

Ivanović N, Minić R, Dimitrijević L, Radojević-Skodrić S, Živković I, Đorđević B. Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis. in Food & Function. 2015;6(2):558-565.
doi:10.1039/c4fo00843j .

Ivanović, Nevena, Minić, Rajna, Dimitrijević, Ljiljana, Radojević-Skodrić, Sanja, Živković, Irena, Đorđević, Brižita, "Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis" in Food & Function, 6, no. 2 (2015):558-565,
https://doi.org/10.1039/c4fo00843j . .

TY  - JOUR
AU  - Ivanović, Nevena
AU  - Minić, Rajna
AU  - Đuričić, Ivana
AU  - Dimitrijević, Ljiljana
AU  - Šobajić, Slađana
AU  - Živković, Irena
AU  - Đorđević, Brižita
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/443
AB  - Recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. There are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. This study was designed to test the effects of a particular Lactobacillus strain, Lactobacillus rhamnosus LA68 on fatty acid composition of the liver and the brain of C57BL/6 mice in the absence of an underlying pathological condition. Female mice were supplemented with live L. rhamnosus LA68 bacteria for the duration of 1 month. Serum biochemistry was analyzed and liver and brain fatty acid composition was assessed by gas-liquid chromatography. Significant changes in liver and brain fatty acid composition were detected. In the liver tissue we detected an increase in palmitoleic acid (p = 0.038), while in the brain compartment we found an increase in palmitic (p = 0.042), stearic (p = 0.017), arachidonic acid (p = 0.009) and docosahexaenoic acid (p = 0.004) for control versus experimental group. These results show discrete changes caused by LA68 strain consumption. Even short duration of administration of LA68 influences the fatty acid composition of the host which adds to the existing knowledge about Lactobacillus host interaction, and adds to the growing knowledge of metabolic intervention possibilities.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Food Sciences and Nutrition
T1  - Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68
EP  - 97
IS  - 1
SP  - 93
VL  - 66
DO  - 10.3109/09637486.2014.979313
ER  - 

@article{
author = "Ivanović, Nevena and Minić, Rajna and Đuričić, Ivana and Dimitrijević, Ljiljana and Šobajić, Slađana and Živković, Irena and Đorđević, Brižita",
year = "2015",
abstract = "Recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. There are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. This study was designed to test the effects of a particular Lactobacillus strain, Lactobacillus rhamnosus LA68 on fatty acid composition of the liver and the brain of C57BL/6 mice in the absence of an underlying pathological condition. Female mice were supplemented with live L. rhamnosus LA68 bacteria for the duration of 1 month. Serum biochemistry was analyzed and liver and brain fatty acid composition was assessed by gas-liquid chromatography. Significant changes in liver and brain fatty acid composition were detected. In the liver tissue we detected an increase in palmitoleic acid (p = 0.038), while in the brain compartment we found an increase in palmitic (p = 0.042), stearic (p = 0.017), arachidonic acid (p = 0.009) and docosahexaenoic acid (p = 0.004) for control versus experimental group. These results show discrete changes caused by LA68 strain consumption. Even short duration of administration of LA68 influences the fatty acid composition of the host which adds to the existing knowledge about Lactobacillus host interaction, and adds to the growing knowledge of metabolic intervention possibilities.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Food Sciences and Nutrition",
title = "Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68",
pages = "97-93",
number = "1",
volume = "66",
doi = "10.3109/09637486.2014.979313"
}

Ivanović, N., Minić, R., Đuričić, I., Dimitrijević, L., Šobajić, S., Živković, I.,& Đorđević, B.. (2015). Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68. in International Journal of Food Sciences and Nutrition
Taylor & Francis Ltd, Abingdon., 66(1), 93-97.
https://doi.org/10.3109/09637486.2014.979313

Ivanović N, Minić R, Đuričić I, Dimitrijević L, Šobajić S, Živković I, Đorđević B. Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68. in International Journal of Food Sciences and Nutrition. 2015;66(1):93-97.
doi:10.3109/09637486.2014.979313 .

Ivanović, Nevena, Minić, Rajna, Đuričić, Ivana, Dimitrijević, Ljiljana, Šobajić, Slađana, Živković, Irena, Đorđević, Brižita, "Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68" in International Journal of Food Sciences and Nutrition, 66, no. 1 (2015):93-97,
https://doi.org/10.3109/09637486.2014.979313 . .

TY  - JOUR
AU  - Dimitrijević, Rajna
AU  - Ivanović, Nevena
AU  - Mathiesen, Geir
AU  - Petrušić, Vladimir
AU  - Živković, Irena
AU  - Đorđević, Brižita
AU  - Dimitrijević, Ljiljana
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/398
AB  - Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-gamma, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-gamma and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.
PB  - Cambridge Univ Press, New York
T2  - Journal of Dairy Research
T1  - Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration
EP  - 207
IS  - 2
SP  - 202
VL  - 81
DO  - 10.1017/S0022029914000028
ER  - 

@article{
author = "Dimitrijević, Rajna and Ivanović, Nevena and Mathiesen, Geir and Petrušić, Vladimir and Živković, Irena and Đorđević, Brižita and Dimitrijević, Ljiljana",
year = "2014",
abstract = "Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-gamma, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-gamma and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.",
publisher = "Cambridge Univ Press, New York",
journal = "Journal of Dairy Research",
title = "Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration",
pages = "207-202",
number = "2",
volume = "81",
doi = "10.1017/S0022029914000028"
}

Dimitrijević, R., Ivanović, N., Mathiesen, G., Petrušić, V., Živković, I., Đorđević, B.,& Dimitrijević, L.. (2014). Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration. in Journal of Dairy Research
Cambridge Univ Press, New York., 81(2), 202-207.
https://doi.org/10.1017/S0022029914000028

Dimitrijević R, Ivanović N, Mathiesen G, Petrušić V, Živković I, Đorđević B, Dimitrijević L. Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration. in Journal of Dairy Research. 2014;81(2):202-207.
doi:10.1017/S0022029914000028 .

Dimitrijević, Rajna, Ivanović, Nevena, Mathiesen, Geir, Petrušić, Vladimir, Živković, Irena, Đorđević, Brižita, Dimitrijević, Ljiljana, "Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration" in Journal of Dairy Research, 81, no. 2 (2014):202-207,
https://doi.org/10.1017/S0022029914000028 . .

TY  - CONF
AU  - Dimitrijević, Rajna
AU  - Ivanović, Nevena
AU  - Mathiesen, Geir
AU  - Petrušić, Vladimir
AU  - Živković, Irena
AU  - Đorđević, Brižita
AU  - Dimitrijević, Ljiljana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/712
AB  - Uvod: Probiotici se koriste vekovima kao deo humane ishrane i u poslednje vreme pridaje im se sve vise značaja. Cilj ovog rada bio je ispitivanje imunoloških aspekata konzumiranja soja Lactobacillus rhamnosus LA68.
Metodologija: Soj LA68 davan je miševima oralno, gavažom četiri puta nedeljno tokom
mesec dana. Korišćena je sveža prekonoćna kultura i jedna doza sastojala se od 2x109
CFU. Nakon isteka eksperimentalnog perioda izolovani su mišji splenociti i analizirani
pomoću protocne citometrije. Meren je i odgovor splenocita u vidu sekrecije citokina
(IFN-y, IL-6, IL-10 and IL-17) kao i proliferacija nakon stimulacije lipopolisaharidom i peptidoglikanom. Analizirani su i citokini u serumu eksperimentalnih miševa. Zdravstveno
stanje životinja praćeno je merenjem težine i osnovnih biohemijskih parametara.
Rezultati: Oralna aplikacija soja LA68 dovodi do značajnog smanjenja zastupljenosti
CD3+, CD25+ i CD19+ celija i do povecanja CD11b+ and CD16/CD32+ ćelija u slezini
eksperimentalnih miševa. Detektovana je i povećana osetljivost na stimulaciju merenjem ćelijske proliferacije i sekrecije IL-6. U serumu eksperimentalnih miševa detektovan
je poviseni nivo IFN-y i smanjenje IL-10. Težina životinja i biohemijski parametri nisu se
razlikovali među grupama i ulazili su u opseg normalnih vrednosti za ovu vrstu miša.
Zaključak: Dobijeni rezultati ukazuju na povećanu osetljivost splenocita na stimulaciju,
aktivaciju Th1 tipa imunskog odgovora i pomeranje leukocitne populacije ka monocitno/granulocitnoj populaciji. Detektovane promene mogu pomoći organizmu u odbrani
protiv određenih vrsta infekcija i umanjiti simtome alergije.
AB  - Introduction: Probiotic bacteria have been used in human nutrition for centuries and
are attracting more attention in recent years. The aim of this study was to evaluate the
effects of Lactobacillus rhamnosus LA68 on the immune system of mice.
Methodology: In order to examine the immunological aspects of probiotic consumption LA68 was orally administrated via gavage to C57 BL/6 mice. Gavage was performed
four times per week for the duration of one month. Fresh overnight culture was used
and one dose consisted of 2x109 CFU. Upon completion mouse splenocytes were isolated and analyzed by flow cytometry. The magnitude of splenocyte proliferation upon
stimulation with LPS and peptidoglycan and cytokine levels (IFN-y, IL-6, IL-10 and IL-17)
were assessed. Cytokine levels in the serum were also analyzed. Mouse well being was
monitored with weight measurement and basic blood biochemisty.
Results: Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+
and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations
in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6
secretion was detected. Increased serum IFN-y and decreased IL-10 levels were found.
Animal weight and biochemical parameters did not differ between the groups and were
within the normal values for this mouse strain.
Conclusion: Our results show increased responsiveness of splenocytes, activation of
the Th1 type of immune response, and a shift of leukocyte populations toward monocyte/granulocyte populations. Changes detected consist of a boost in defense mechanisms against certain types of bacteria and ameliorate allergy symptoms.
PB  - Udruženje za medicinu sporta Srbije
PB  - Univerzitet u Beogradu, Farmaceutski fakultet - Institut za bromatologiju
C3  - Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija
T1  - Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa
T1  - Effects of l.rhamnosus la68 on the immune system of c57 bl/6 mice upon oral administration
SP  - 12
UR  - https://hdl.handle.net/21.15107/rcub_intor_712
ER  - 

@conference{
author = "Dimitrijević, Rajna and Ivanović, Nevena and Mathiesen, Geir and Petrušić, Vladimir and Živković, Irena and Đorđević, Brižita and Dimitrijević, Ljiljana",
year = "2013",
abstract = "Uvod: Probiotici se koriste vekovima kao deo humane ishrane i u poslednje vreme pridaje im se sve vise značaja. Cilj ovog rada bio je ispitivanje imunoloških aspekata konzumiranja soja Lactobacillus rhamnosus LA68.
Metodologija: Soj LA68 davan je miševima oralno, gavažom četiri puta nedeljno tokom
mesec dana. Korišćena je sveža prekonoćna kultura i jedna doza sastojala se od 2x109
CFU. Nakon isteka eksperimentalnog perioda izolovani su mišji splenociti i analizirani
pomoću protocne citometrije. Meren je i odgovor splenocita u vidu sekrecije citokina
(IFN-y, IL-6, IL-10 and IL-17) kao i proliferacija nakon stimulacije lipopolisaharidom i peptidoglikanom. Analizirani su i citokini u serumu eksperimentalnih miševa. Zdravstveno
stanje životinja praćeno je merenjem težine i osnovnih biohemijskih parametara.
Rezultati: Oralna aplikacija soja LA68 dovodi do značajnog smanjenja zastupljenosti
CD3+, CD25+ i CD19+ celija i do povecanja CD11b+ and CD16/CD32+ ćelija u slezini
eksperimentalnih miševa. Detektovana je i povećana osetljivost na stimulaciju merenjem ćelijske proliferacije i sekrecije IL-6. U serumu eksperimentalnih miševa detektovan
je poviseni nivo IFN-y i smanjenje IL-10. Težina životinja i biohemijski parametri nisu se
razlikovali među grupama i ulazili su u opseg normalnih vrednosti za ovu vrstu miša.
Zaključak: Dobijeni rezultati ukazuju na povećanu osetljivost splenocita na stimulaciju,
aktivaciju Th1 tipa imunskog odgovora i pomeranje leukocitne populacije ka monocitno/granulocitnoj populaciji. Detektovane promene mogu pomoći organizmu u odbrani
protiv određenih vrsta infekcija i umanjiti simtome alergije., Introduction: Probiotic bacteria have been used in human nutrition for centuries and
are attracting more attention in recent years. The aim of this study was to evaluate the
effects of Lactobacillus rhamnosus LA68 on the immune system of mice.
Methodology: In order to examine the immunological aspects of probiotic consumption LA68 was orally administrated via gavage to C57 BL/6 mice. Gavage was performed
four times per week for the duration of one month. Fresh overnight culture was used
and one dose consisted of 2x109 CFU. Upon completion mouse splenocytes were isolated and analyzed by flow cytometry. The magnitude of splenocyte proliferation upon
stimulation with LPS and peptidoglycan and cytokine levels (IFN-y, IL-6, IL-10 and IL-17)
were assessed. Cytokine levels in the serum were also analyzed. Mouse well being was
monitored with weight measurement and basic blood biochemisty.
Results: Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+
and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations
in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6
secretion was detected. Increased serum IFN-y and decreased IL-10 levels were found.
Animal weight and biochemical parameters did not differ between the groups and were
within the normal values for this mouse strain.
Conclusion: Our results show increased responsiveness of splenocytes, activation of
the Th1 type of immune response, and a shift of leukocyte populations toward monocyte/granulocyte populations. Changes detected consist of a boost in defense mechanisms against certain types of bacteria and ameliorate allergy symptoms.",
publisher = "Udruženje za medicinu sporta Srbije, Univerzitet u Beogradu, Farmaceutski fakultet - Institut za bromatologiju",
journal = "Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija",
title = "Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa, Effects of l.rhamnosus la68 on the immune system of c57 bl/6 mice upon oral administration",
pages = "12",
url = "https://hdl.handle.net/21.15107/rcub_intor_712"
}

Dimitrijević, R., Ivanović, N., Mathiesen, G., Petrušić, V., Živković, I., Đorđević, B.,& Dimitrijević, L.. (2013). Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa. in Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija
Udruženje za medicinu sporta Srbije., 12.
https://hdl.handle.net/21.15107/rcub_intor_712

Dimitrijević R, Ivanović N, Mathiesen G, Petrušić V, Živković I, Đorđević B, Dimitrijević L. Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa. in Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija. 2013;:12.
https://hdl.handle.net/21.15107/rcub_intor_712 .

Dimitrijević, Rajna, Ivanović, Nevena, Mathiesen, Geir, Petrušić, Vladimir, Živković, Irena, Đorđević, Brižita, Dimitrijević, Ljiljana, "Efekti oralne primene l. Rhamnosus la68 na imuni sistem c57 bl/6 miševa" in Četvrti kongres o dietetskim suplementima sa međunarodnim učešćem, Suplementacija zasnovana na dokazima, 24-25. oktobar 2013. Beograd, Srbija (2013):12,
https://hdl.handle.net/21.15107/rcub_intor_712 .