TY  - JOUR
AU  - Šeatović, Saša S.
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Živković, Irena
AU  - Jankov, Ratko
AU  - Dimitrijević, Ljiljana
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/181
AB  - According to the recommendation of the World Health Organization (WHO), the use of an in vivo test for measuring of the potency of tetanus toxoid vaccine (TTdV) is still unavoidable, but the establishment of a convenient in vitro test would significantly improve the work in this field. A sandwich enzyme-linked immunosorbent assay (sELISA) was developed for a rapid and sensitive quantification of tetanus toxoid (TTd). We produced four monoclonal antibodies (MAbs) designated 41, 51, 62, and 71 that reacted with TTd and recognized, different antigenic determinants on TTd. We also used two of these antibodies for developing a sELISA, with MoAb 71 as an immobilized and MoAb 51 as a capture antibody. The measurement range of this assay was from 31-1000 ng/mL and the minimum detection limit for TTd was 31 ng/mL. This high sensitivity of this sELISA and its good reproducibility suggest that the developed method could be reliably used to estimate the concentration of TTd, which could be easily extrapolated to the estimation of vaccine potency.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Immunoassay & Immunochemistry
T1  - Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration
EP  - 44
IS  - 1
SP  - 31
VL  - 25
DO  - 10.1081/IAS-120027224
ER  - 

@article{
author = "Šeatović, Saša S. and Inić-Kanada, Aleksandra and Stojanović, Marijana and Živković, Irena and Jankov, Ratko and Dimitrijević, Ljiljana",
year = "2004",
abstract = "According to the recommendation of the World Health Organization (WHO), the use of an in vivo test for measuring of the potency of tetanus toxoid vaccine (TTdV) is still unavoidable, but the establishment of a convenient in vitro test would significantly improve the work in this field. A sandwich enzyme-linked immunosorbent assay (sELISA) was developed for a rapid and sensitive quantification of tetanus toxoid (TTd). We produced four monoclonal antibodies (MAbs) designated 41, 51, 62, and 71 that reacted with TTd and recognized, different antigenic determinants on TTd. We also used two of these antibodies for developing a sELISA, with MoAb 71 as an immobilized and MoAb 51 as a capture antibody. The measurement range of this assay was from 31-1000 ng/mL and the minimum detection limit for TTd was 31 ng/mL. This high sensitivity of this sELISA and its good reproducibility suggest that the developed method could be reliably used to estimate the concentration of TTd, which could be easily extrapolated to the estimation of vaccine potency.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Immunoassay & Immunochemistry",
title = "Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration",
pages = "44-31",
number = "1",
volume = "25",
doi = "10.1081/IAS-120027224"
}

Šeatović, S. S., Inić-Kanada, A., Stojanović, M., Živković, I., Jankov, R.,& Dimitrijević, L.. (2004). Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration. in Journal of Immunoassay & Immunochemistry
Taylor & Francis Inc, Philadelphia., 25(1), 31-44.
https://doi.org/10.1081/IAS-120027224

Šeatović SS, Inić-Kanada A, Stojanović M, Živković I, Jankov R, Dimitrijević L. Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration. in Journal of Immunoassay & Immunochemistry. 2004;25(1):31-44.
doi:10.1081/IAS-120027224 .

Šeatović, Saša S., Inić-Kanada, Aleksandra, Stojanović, Marijana, Živković, Irena, Jankov, Ratko, Dimitrijević, Ljiljana, "Development of sandwich enzyme-linked immunosorbent assay for determination of tetanus toxoid concentration" in Journal of Immunoassay & Immunochemistry, 25, no. 1 (2004):31-44,
https://doi.org/10.1081/IAS-120027224 . .

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Šeatović, Saša S.
AU  - Živković, Irena
AU  - Jankov, Ratko
AU  - Živančević-Simonović, Snežana
AU  - Dimitrijević, Ljiljana
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/154
AB  - Monoclonal antibodies (MoAb) are usually produced as the secretion products of cloned B lymphoblastoid cell lines. Advantages of MoAbs are that they could be selected to have desired specificity and produced in relatively large quantities of consistent quality and char acteristics. Interest in the production of re agents specific for human kappa (K) chain can be explained by the fact that many pathological conditions are accompanied by frequency of immunoglobulins with K light chain. The aim of this study was production of stable MoAb-se creating hybridoma with ability to secrete imunoglobulin specific for human K chain, immunochemical characterization of this MoAb and its possible application. Murine monoclonal antibody, as signed as MoAt 44, produced by hybridoma technology is specific for human kappa chain and fully immunochemically characterized. In this paper, we reported that MoAb 44 demonstrated excellent properties in most immunochemical techniques (immunoblot, dot-blot, immunofluorescence, double and radial immunodiffusion, immunoelectrophoresis...), which highly recommend this MoAb for the application as a tool for re search and immunodiagnostics.
AB  - Monoklonska antitela (MoAt) su sekretorni proizvodi kloniranih B ćelijskih limfoblastoidnih linija. Od poliklonskih antitela ih razlikuje: definisana specifičnost vezivanja, homogenost i mogućnost proizvodnje u velikoj količini. Budući da su brojna patološka stanja povezana sa sekrecijom imunoglobulina u čijem sastavu se nalazi kapa (k) tip lakog lanca, proizvodnja specifičnih monoklonskih antitela našla bi svoje mesto u njihovoj dijagnostici. Stoga je cilj našeg rada bio dobijanje stabilnog mišjeg hibridomskog klona koji bi sekretovao MoAt specifično za humani k lanac, imunohemijska karakterizacija tog MoAt i ispitivanje mogućnosti njegove primene. Mišje monoklonsko antitelo, označeno kao MoAt 44, proizvedeno hibridomskom tehnologijom, specifično je za k lanac humanih imunoglobulina i u potpunosti imunohemijski okarakterisano. MoAt 44 može da se koristi u većini imunohemijskih tehnika (imunoblot-u, dot-blot-u, imunofluorescenci, dvostrukoj i radijalnoj imunodifuziji, imunoelektroforezi...), što omogućava njegovu primenu u naučno-istraživačkom radu i imunodijagnostici.
PB  - Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac
T2  - Medicus
T1  - The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities
T1  - Mišje monoklonsko antitelo specifično za kapa lanac humanih imunoglobulina i mogućnosti njegove primene
EP  - 25
IS  - 1
SP  - 21
VL  - 3
UR  - https://hdl.handle.net/21.15107/rcub_intor_154
ER  - 

@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Šeatović, Saša S. and Živković, Irena and Jankov, Ratko and Živančević-Simonović, Snežana and Dimitrijević, Ljiljana",
year = "2002",
abstract = "Monoclonal antibodies (MoAb) are usually produced as the secretion products of cloned B lymphoblastoid cell lines. Advantages of MoAbs are that they could be selected to have desired specificity and produced in relatively large quantities of consistent quality and char acteristics. Interest in the production of re agents specific for human kappa (K) chain can be explained by the fact that many pathological conditions are accompanied by frequency of immunoglobulins with K light chain. The aim of this study was production of stable MoAb-se creating hybridoma with ability to secrete imunoglobulin specific for human K chain, immunochemical characterization of this MoAb and its possible application. Murine monoclonal antibody, as signed as MoAt 44, produced by hybridoma technology is specific for human kappa chain and fully immunochemically characterized. In this paper, we reported that MoAb 44 demonstrated excellent properties in most immunochemical techniques (immunoblot, dot-blot, immunofluorescence, double and radial immunodiffusion, immunoelectrophoresis...), which highly recommend this MoAb for the application as a tool for re search and immunodiagnostics., Monoklonska antitela (MoAt) su sekretorni proizvodi kloniranih B ćelijskih limfoblastoidnih linija. Od poliklonskih antitela ih razlikuje: definisana specifičnost vezivanja, homogenost i mogućnost proizvodnje u velikoj količini. Budući da su brojna patološka stanja povezana sa sekrecijom imunoglobulina u čijem sastavu se nalazi kapa (k) tip lakog lanca, proizvodnja specifičnih monoklonskih antitela našla bi svoje mesto u njihovoj dijagnostici. Stoga je cilj našeg rada bio dobijanje stabilnog mišjeg hibridomskog klona koji bi sekretovao MoAt specifično za humani k lanac, imunohemijska karakterizacija tog MoAt i ispitivanje mogućnosti njegove primene. Mišje monoklonsko antitelo, označeno kao MoAt 44, proizvedeno hibridomskom tehnologijom, specifično je za k lanac humanih imunoglobulina i u potpunosti imunohemijski okarakterisano. MoAt 44 može da se koristi u većini imunohemijskih tehnika (imunoblot-u, dot-blot-u, imunofluorescenci, dvostrukoj i radijalnoj imunodifuziji, imunoelektroforezi...), što omogućava njegovu primenu u naučno-istraživačkom radu i imunodijagnostici.",
publisher = "Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac",
journal = "Medicus",
title = "The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities, Mišje monoklonsko antitelo specifično za kapa lanac humanih imunoglobulina i mogućnosti njegove primene",
pages = "25-21",
number = "1",
volume = "3",
url = "https://hdl.handle.net/21.15107/rcub_intor_154"
}

Inić-Kanada, A., Stojanović, M., Šeatović, S. S., Živković, I., Jankov, R., Živančević-Simonović, S.,& Dimitrijević, L.. (2002). The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities. in Medicus
Univerzitet u Kragujevcu - Fakultet medicinskih nauka, Kragujevac., 3(1), 21-25.
https://hdl.handle.net/21.15107/rcub_intor_154

Inić-Kanada A, Stojanović M, Šeatović SS, Živković I, Jankov R, Živančević-Simonović S, Dimitrijević L. The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities. in Medicus. 2002;3(1):21-25.
https://hdl.handle.net/21.15107/rcub_intor_154 .

Inić-Kanada, Aleksandra, Stojanović, Marijana, Šeatović, Saša S., Živković, Irena, Jankov, Ratko, Živančević-Simonović, Snežana, Dimitrijević, Ljiljana, "The murine monoclonal antibody specific for human kappa immunoglobuline chain and its application possibilities" in Medicus, 3, no. 1 (2002):21-25,
https://hdl.handle.net/21.15107/rcub_intor_154 .