Đuričić, Bosiljka

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Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia

Đuričić, Bosiljka; Vasić, Ana; Rogožarski, Dragan; Vojinović, Dragica; Elezović-Radovanović, Milica; Manić, Marija; Marić, Jovan; Prokić, Nataša; Ilić, Živka; Novotny, N.; Gligić, Ana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2013)

TY  - JOUR
AU  - Đuričić, Bosiljka
AU  - Vasić, Ana
AU  - Rogožarski, Dragan
AU  - Vojinović, Dragica
AU  - Elezović-Radovanović, Milica
AU  - Manić, Marija
AU  - Marić, Jovan
AU  - Prokić, Nataša
AU  - Ilić, Živka
AU  - Novotny, N.
AU  - Gligić, Ana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/366
AB  - The disease caused by the West Nile virus (WNV) has been known since 1937 when it was described for the first time in Uganda. After spreading to Europe and the Middle East, the disease has changed its primary location. Today WN infection is a significant health problem in the world. As a result of the current epizootiological and epidemiological situation in Europe studies on the occurrence of WND were introduced in Serbia, also. The investigation on the presence of WNV antibodies was intensified in the period from 2008 to 2012. In this period a total of 3618 serum samples were tested from 18 localities (2736 animal sera from 8 different species and 882 human sera samples). The method applied was gel immunodiffusion and the representative samples were confirmed by the plaque reduction test (PRNT-90). Out of the total number of samples WNV antibodies were present in 3.97% horses, 0.93% dogs, 0.31% poultry and 1.36% man. In one horse serum sample there was a positive reaction with a positive control serum, thus indicating the presence of WNV antigen. The results have confirmed that WNV antibodies are present in 9 out of 18 tested locations in the Republic of Serbia. The percentage of seropositive samples varies from 0.42% in Pozarevac (horses and humans) up to 6.45% in Novi Pazar (dogs). Out of the investigated species the highest seropositivity was recorded in horses (3.97%), and lowest in poultry (0.31%). WNV is present and widespread in the Republic of Serbia, thus enabling distribution mapping.
AB  - Bolest koju izaziva virus Zapadnog Nila (WNV) je poznata još od 1937. kada je po prvi put opisana u Ugandi. Posle širenja virusa u Evropu i na Bliski istok, bolest je promenila prvobitnu lokaciju. Danas je WN infekcija značajan zdravstveni problem u svetu. Kao rezultat trenutne epizootiološko-epidemiološke situacije u Evropi, uvedena su istraživanja prisustva WNV i u Srbiji. Istraživanja prisustva antitela za WNV intenzivirana su u periodu od 2008. do 2012. godine. U ovom periodu ukupno je ispitano 3618 seruma sa 18 lokaliteta (2736 seruma životinja, od 8 različitih vrsta i 882 seruma ljudi). Korišćen je metod imunodifuzije u gelu a reprezentativni uzorak potvrđen je testom neutralizacije plaka (PRNT- 90). Od ukupnog broja uzoraka na prisustvo antitela za WNV bilo je pozitivno 3,97% konja, 0,93% pasa, 0,31% domaće živine i 1,36% ljudi. U serumu jednog konja pojavila se pozitivna reakcija sa pozitivnim kontrolnim serumom, ukazujući na prisustvo antigena WNV. Dobijeni rezultati su potvrdili da su antitela za WNV prisutna u 9 od 18 ispitivanih lokacija u Republici Srbiji. Procenat seropozitivnih varira od 0.42% u Požarevcu (konji i ljudi) do 6,45% u Novom Pazaru (psi). Od ispitivanih vrsta najveća seropozitivnost je registrovana kod konja (3,97%), a najniža kod živine (0,31%). Raspoloživi podaci ukazuju da je WNV prisutan i značajno raširen u Republici Srbiji, što je omogućilo i mapiranje njegovog širenja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia
T1  - Seroepizootiološko-epidemiološka istraživanja i mapiranje infekcije Zapadnog Nila u Republici Srbiji
EP  - 579
IS  - 5-6
SP  - 569
VL  - 63
DO  - 10.2298/AVB1306569D
ER  - 
@article{
author = "Đuričić, Bosiljka and Vasić, Ana and Rogožarski, Dragan and Vojinović, Dragica and Elezović-Radovanović, Milica and Manić, Marija and Marić, Jovan and Prokić, Nataša and Ilić, Živka and Novotny, N. and Gligić, Ana",
year = "2013",
abstract = "The disease caused by the West Nile virus (WNV) has been known since 1937 when it was described for the first time in Uganda. After spreading to Europe and the Middle East, the disease has changed its primary location. Today WN infection is a significant health problem in the world. As a result of the current epizootiological and epidemiological situation in Europe studies on the occurrence of WND were introduced in Serbia, also. The investigation on the presence of WNV antibodies was intensified in the period from 2008 to 2012. In this period a total of 3618 serum samples were tested from 18 localities (2736 animal sera from 8 different species and 882 human sera samples). The method applied was gel immunodiffusion and the representative samples were confirmed by the plaque reduction test (PRNT-90). Out of the total number of samples WNV antibodies were present in 3.97% horses, 0.93% dogs, 0.31% poultry and 1.36% man. In one horse serum sample there was a positive reaction with a positive control serum, thus indicating the presence of WNV antigen. The results have confirmed that WNV antibodies are present in 9 out of 18 tested locations in the Republic of Serbia. The percentage of seropositive samples varies from 0.42% in Pozarevac (horses and humans) up to 6.45% in Novi Pazar (dogs). Out of the investigated species the highest seropositivity was recorded in horses (3.97%), and lowest in poultry (0.31%). WNV is present and widespread in the Republic of Serbia, thus enabling distribution mapping., Bolest koju izaziva virus Zapadnog Nila (WNV) je poznata još od 1937. kada je po prvi put opisana u Ugandi. Posle širenja virusa u Evropu i na Bliski istok, bolest je promenila prvobitnu lokaciju. Danas je WN infekcija značajan zdravstveni problem u svetu. Kao rezultat trenutne epizootiološko-epidemiološke situacije u Evropi, uvedena su istraživanja prisustva WNV i u Srbiji. Istraživanja prisustva antitela za WNV intenzivirana su u periodu od 2008. do 2012. godine. U ovom periodu ukupno je ispitano 3618 seruma sa 18 lokaliteta (2736 seruma životinja, od 8 različitih vrsta i 882 seruma ljudi). Korišćen je metod imunodifuzije u gelu a reprezentativni uzorak potvrđen je testom neutralizacije plaka (PRNT- 90). Od ukupnog broja uzoraka na prisustvo antitela za WNV bilo je pozitivno 3,97% konja, 0,93% pasa, 0,31% domaće živine i 1,36% ljudi. U serumu jednog konja pojavila se pozitivna reakcija sa pozitivnim kontrolnim serumom, ukazujući na prisustvo antigena WNV. Dobijeni rezultati su potvrdili da su antitela za WNV prisutna u 9 od 18 ispitivanih lokacija u Republici Srbiji. Procenat seropozitivnih varira od 0.42% u Požarevcu (konji i ljudi) do 6,45% u Novom Pazaru (psi). Od ispitivanih vrsta najveća seropozitivnost je registrovana kod konja (3,97%), a najniža kod živine (0,31%). Raspoloživi podaci ukazuju da je WNV prisutan i značajno raširen u Republici Srbiji, što je omogućilo i mapiranje njegovog širenja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia, Seroepizootiološko-epidemiološka istraživanja i mapiranje infekcije Zapadnog Nila u Republici Srbiji",
pages = "579-569",
number = "5-6",
volume = "63",
doi = "10.2298/AVB1306569D"
}
Đuričić, B., Vasić, A., Rogožarski, D., Vojinović, D., Elezović-Radovanović, M., Manić, M., Marić, J., Prokić, N., Ilić, Ž., Novotny, N.,& Gligić, A.. (2013). Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 569-579.
https://doi.org/10.2298/AVB1306569D
Đuričić B, Vasić A, Rogožarski D, Vojinović D, Elezović-Radovanović M, Manić M, Marić J, Prokić N, Ilić Ž, Novotny N, Gligić A. Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia. in Acta veterinaria - Beograd. 2013;63(5-6):569-579.
doi:10.2298/AVB1306569D .
Đuričić, Bosiljka, Vasić, Ana, Rogožarski, Dragan, Vojinović, Dragica, Elezović-Radovanović, Milica, Manić, Marija, Marić, Jovan, Prokić, Nataša, Ilić, Živka, Novotny, N., Gligić, Ana, "Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia" in Acta veterinaria - Beograd, 63, no. 5-6 (2013):569-579,
https://doi.org/10.2298/AVB1306569D . .
5
3
4

Arboviruses in Serbia and in the world

Gligić, Ana; Đuričić, Bosiljka

(Most Art D.O.O., Beograd, 2011)

TY  - JOUR
AU  - Gligić, Ana
AU  - Đuričić, Bosiljka
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/336
AB  - Arboviruses are the most numerous group of viruses with 534 registered members. With a few exceptions, most of arboviruses are RNA viruses. Mostly they have zoonotic characteristics and are transmissible by vectors- arthropods (ticks, mosquitoes, phlebothomines etc.) on human and animal population. Arboviruses induced epidemics and epizooties have massive occurrence with huge economic loses. Primarily, arboviruses are neurotropic viruses that cause damage on central nervous system with lethality rate 5-80%, and are considered to be very dangerous- biohazard. Beside vector transmission interhuman transmission can occur (Crimean-Congo hemorrhagic fever, Ebola, Lasso, Marburg). According to their characteristics, some of arboviruses took place in the List of biological weapons used against humans, animals and even plants. Investigation of Arboviruses in the Republic of Serbia has been started in 1960s, resulting with isolation of 5 arboviruses - 4 were isolated from vectors samples and one from human material sample. Two of them under the name of Jug Bogdanovac and Dubrava/Belgrade took place in the International catalogue of viruses as a new isolated arboviruses. The formation of distribution map of Arboviruses in Serbia was started by isolations and seroepidemiological investigations as well as overview of their impact to human public and veterinary medicine health.
AB  - Arbovirusi su najbrojnija grupa virusa sa više od 534 registrovana člana. Sa malim izuzetkom to su RNK virusi. Većina su zoonoznog karaktera, a na ljude i životinje prenose se posredstvom artropoda (krpelja, komaraca, flebotomina). Epidemije i epizootije koje izazivaju su masovne sa velikim ekonomskim gubicima u zdravstvu i ekonomiji zemlje. Primarno su neurotropni i izazivaju oštećenja centralnog nervnog sistema sa letalitetom od 5-80%. Neki su veoma opasni - biohazardni i pored vektorskog prenošenja prenose se i intrahumano (Krimska - Kongo HG, Ebola, Lassa, Marburg i druge). Zbog svojih osobina neki arbovirusi su i na listi biološkog oružja protiv ljudi, životinja, pa čak i bilja. Istraživanja arbovirusa u Srbiji započeta su 1960 godine, a rezultirala su izolacijom 5 arbovirusa i to 4 iz vektora (Krpelja, komaraca i flebotomina), od kojih je jedan kao nov pod imenom Jug Bogdanovac ušao u međunarodni katalog, i jedan iz humanog materijala koji je pod imenom Dobrava/Belgrade takođe registrovan u međunarodnom katalogu arbovirusa. Izolacijama virusa i seroepidemiološkim istraživanjima započeto je formiranje karte rasprostranjenosti arbovirusa u Srbiji i sagledavanje njihove uloge u zdravstvu i veterinarskoj medicine.
PB  - Most Art D.O.O., Beograd
T2  - MD - Medical data
T1  - Arboviruses in Serbia and in the world
T1  - Arbovirusi kod nas i u svetu
EP  - 381
IS  - 4
SP  - 379
VL  - 3
UR  - https://hdl.handle.net/21.15107/rcub_intor_336
ER  - 
@article{
author = "Gligić, Ana and Đuričić, Bosiljka",
year = "2011",
abstract = "Arboviruses are the most numerous group of viruses with 534 registered members. With a few exceptions, most of arboviruses are RNA viruses. Mostly they have zoonotic characteristics and are transmissible by vectors- arthropods (ticks, mosquitoes, phlebothomines etc.) on human and animal population. Arboviruses induced epidemics and epizooties have massive occurrence with huge economic loses. Primarily, arboviruses are neurotropic viruses that cause damage on central nervous system with lethality rate 5-80%, and are considered to be very dangerous- biohazard. Beside vector transmission interhuman transmission can occur (Crimean-Congo hemorrhagic fever, Ebola, Lasso, Marburg). According to their characteristics, some of arboviruses took place in the List of biological weapons used against humans, animals and even plants. Investigation of Arboviruses in the Republic of Serbia has been started in 1960s, resulting with isolation of 5 arboviruses - 4 were isolated from vectors samples and one from human material sample. Two of them under the name of Jug Bogdanovac and Dubrava/Belgrade took place in the International catalogue of viruses as a new isolated arboviruses. The formation of distribution map of Arboviruses in Serbia was started by isolations and seroepidemiological investigations as well as overview of their impact to human public and veterinary medicine health., Arbovirusi su najbrojnija grupa virusa sa više od 534 registrovana člana. Sa malim izuzetkom to su RNK virusi. Većina su zoonoznog karaktera, a na ljude i životinje prenose se posredstvom artropoda (krpelja, komaraca, flebotomina). Epidemije i epizootije koje izazivaju su masovne sa velikim ekonomskim gubicima u zdravstvu i ekonomiji zemlje. Primarno su neurotropni i izazivaju oštećenja centralnog nervnog sistema sa letalitetom od 5-80%. Neki su veoma opasni - biohazardni i pored vektorskog prenošenja prenose se i intrahumano (Krimska - Kongo HG, Ebola, Lassa, Marburg i druge). Zbog svojih osobina neki arbovirusi su i na listi biološkog oružja protiv ljudi, životinja, pa čak i bilja. Istraživanja arbovirusa u Srbiji započeta su 1960 godine, a rezultirala su izolacijom 5 arbovirusa i to 4 iz vektora (Krpelja, komaraca i flebotomina), od kojih je jedan kao nov pod imenom Jug Bogdanovac ušao u međunarodni katalog, i jedan iz humanog materijala koji je pod imenom Dobrava/Belgrade takođe registrovan u međunarodnom katalogu arbovirusa. Izolacijama virusa i seroepidemiološkim istraživanjima započeto je formiranje karte rasprostranjenosti arbovirusa u Srbiji i sagledavanje njihove uloge u zdravstvu i veterinarskoj medicine.",
publisher = "Most Art D.O.O., Beograd",
journal = "MD - Medical data",
title = "Arboviruses in Serbia and in the world, Arbovirusi kod nas i u svetu",
pages = "381-379",
number = "4",
volume = "3",
url = "https://hdl.handle.net/21.15107/rcub_intor_336"
}
Gligić, A.,& Đuričić, B.. (2011). Arboviruses in Serbia and in the world. in MD - Medical data
Most Art D.O.O., Beograd., 3(4), 379-381.
https://hdl.handle.net/21.15107/rcub_intor_336
Gligić A, Đuričić B. Arboviruses in Serbia and in the world. in MD - Medical data. 2011;3(4):379-381.
https://hdl.handle.net/21.15107/rcub_intor_336 .
Gligić, Ana, Đuričić, Bosiljka, "Arboviruses in Serbia and in the world" in MD - Medical data, 3, no. 4 (2011):379-381,
https://hdl.handle.net/21.15107/rcub_intor_336 .