TY  - JOUR
AU  - Nedeljković, Jasminka
AU  - Jovanović, T.
AU  - Oker-Blom, C.
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/127
AB  - Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus proteins. Altogether, 47 well-characterised serum samples from both naturally infected patients and vaccines were studied. Results: after natural RV infection, IgG antibodies specific for the E1 protein were predominant not only in terms of levels, but also in terms of rate and magnitude of avidity maturation. The avidity development of the IgG antibodies was much slower in vaccines than in patients after a natural RV infection. Conclusions: together, our results indicate that IgG avidity determination in conjunction with immunoblot analysis is useful in the diagnosis of a RV infection. The recombinant proteins showed similar reactivity patterns in the immunoblot analyses as compared with the authentic viral structural proteins, suggesting suitability for serodiagnostics. (C) 2001 Elsevier Science B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Clinical Virology
T1  - Maturation of IgG avidity to individual rubella virus structural proteins
EP  - 54
IS  - 1
SP  - 47
VL  - 22
DO  - 10.1016/S1386-6532(01)00161-5
ER  - 

@article{
author = "Nedeljković, Jasminka and Jovanović, T. and Oker-Blom, C.",
year = "2001",
abstract = "Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus proteins. Altogether, 47 well-characterised serum samples from both naturally infected patients and vaccines were studied. Results: after natural RV infection, IgG antibodies specific for the E1 protein were predominant not only in terms of levels, but also in terms of rate and magnitude of avidity maturation. The avidity development of the IgG antibodies was much slower in vaccines than in patients after a natural RV infection. Conclusions: together, our results indicate that IgG avidity determination in conjunction with immunoblot analysis is useful in the diagnosis of a RV infection. The recombinant proteins showed similar reactivity patterns in the immunoblot analyses as compared with the authentic viral structural proteins, suggesting suitability for serodiagnostics. (C) 2001 Elsevier Science B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Clinical Virology",
title = "Maturation of IgG avidity to individual rubella virus structural proteins",
pages = "54-47",
number = "1",
volume = "22",
doi = "10.1016/S1386-6532(01)00161-5"
}

Nedeljković, J., Jovanović, T.,& Oker-Blom, C.. (2001). Maturation of IgG avidity to individual rubella virus structural proteins. in Journal of Clinical Virology
Elsevier, Amsterdam., 22(1), 47-54.
https://doi.org/10.1016/S1386-6532(01)00161-5

Nedeljković J, Jovanović T, Oker-Blom C. Maturation of IgG avidity to individual rubella virus structural proteins. in Journal of Clinical Virology. 2001;22(1):47-54.
doi:10.1016/S1386-6532(01)00161-5 .

Nedeljković, Jasminka, Jovanović, T., Oker-Blom, C., "Maturation of IgG avidity to individual rubella virus structural proteins" in Journal of Clinical Virology, 22, no. 1 (2001):47-54,
https://doi.org/10.1016/S1386-6532(01)00161-5 . .

TY  - JOUR
AU  - Nedeljković, Jasminka
AU  - Jovanović, T.
AU  - Mlađenović, S.
AU  - Hedman, K.
AU  - Peitsaro, N.
AU  - Oker-Blom, C.
PY  - 1999
UR  - http://intor.torlakinstitut.com/handle/123456789/108
AB  - Background: The three structural proteins of rubella virus (RV), the capsid protein C and the envelope glycoproteins E1 and E2, were produced individually in soluble form in Sf9 insect cells using the baculovirus system. All proteins were equipped with a polyhistidine tag at their C-terminal ends to enable gentle purification by metal ion affinity chromatography. In addition, the E2 and E2 proteins were engineered to display the FLAG epitope tag at their N-terminal ends. Study design: The diagnostic potential of the recombinant purified proteins was evaluated by immunoblot and enzyme immune assays (EIA) using a total of 57 well-characterised serum samples obtained at various time points after natural RV infection, congenital rubella syndrome (C:RS), MMR vaccination or from controls with past RV immunity. In addition, acute and convalescent phase serum pools from a total of 20 patients were evaluated. Authentic RV proteins were used as a reference. Results: The recombinant E1 and C proteins were predominant in eliciting the immune responce in both postnatal and vaccinal RV infections, being much weaker in the vaccinal ones. The IgG responce to the recombinant C protein was very strong after the first month post infection and decreased with time. The immune responce against the recombinant E2 protein, however, was generally poor, but notably stronger after congenital infection. Together, the results showed that the individual recombinant protein antigens could be suitable for diagnosis of RV infection and for study of the immune response to rubella vaccination. (C) 1999 Elsevier Science B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Clinical Virology
T1  - Immunoblot analysis of natural and vaccine-induced IgG responses to rubella virus proteins expressed in insect cells
EP  - 131
IS  - 2
SP  - 119
VL  - 14
DO  - 10.1016/S1386-6532(99)00048-7
ER  - 

@article{
author = "Nedeljković, Jasminka and Jovanović, T. and Mlađenović, S. and Hedman, K. and Peitsaro, N. and Oker-Blom, C.",
year = "1999",
abstract = "Background: The three structural proteins of rubella virus (RV), the capsid protein C and the envelope glycoproteins E1 and E2, were produced individually in soluble form in Sf9 insect cells using the baculovirus system. All proteins were equipped with a polyhistidine tag at their C-terminal ends to enable gentle purification by metal ion affinity chromatography. In addition, the E2 and E2 proteins were engineered to display the FLAG epitope tag at their N-terminal ends. Study design: The diagnostic potential of the recombinant purified proteins was evaluated by immunoblot and enzyme immune assays (EIA) using a total of 57 well-characterised serum samples obtained at various time points after natural RV infection, congenital rubella syndrome (C:RS), MMR vaccination or from controls with past RV immunity. In addition, acute and convalescent phase serum pools from a total of 20 patients were evaluated. Authentic RV proteins were used as a reference. Results: The recombinant E1 and C proteins were predominant in eliciting the immune responce in both postnatal and vaccinal RV infections, being much weaker in the vaccinal ones. The IgG responce to the recombinant C protein was very strong after the first month post infection and decreased with time. The immune responce against the recombinant E2 protein, however, was generally poor, but notably stronger after congenital infection. Together, the results showed that the individual recombinant protein antigens could be suitable for diagnosis of RV infection and for study of the immune response to rubella vaccination. (C) 1999 Elsevier Science B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Clinical Virology",
title = "Immunoblot analysis of natural and vaccine-induced IgG responses to rubella virus proteins expressed in insect cells",
pages = "131-119",
number = "2",
volume = "14",
doi = "10.1016/S1386-6532(99)00048-7"
}

Nedeljković, J., Jovanović, T., Mlađenović, S., Hedman, K., Peitsaro, N.,& Oker-Blom, C.. (1999). Immunoblot analysis of natural and vaccine-induced IgG responses to rubella virus proteins expressed in insect cells. in Journal of Clinical Virology
Elsevier, Amsterdam., 14(2), 119-131.
https://doi.org/10.1016/S1386-6532(99)00048-7

Nedeljković J, Jovanović T, Mlađenović S, Hedman K, Peitsaro N, Oker-Blom C. Immunoblot analysis of natural and vaccine-induced IgG responses to rubella virus proteins expressed in insect cells. in Journal of Clinical Virology. 1999;14(2):119-131.
doi:10.1016/S1386-6532(99)00048-7 .

Nedeljković, Jasminka, Jovanović, T., Mlađenović, S., Hedman, K., Peitsaro, N., Oker-Blom, C., "Immunoblot analysis of natural and vaccine-induced IgG responses to rubella virus proteins expressed in insect cells" in Journal of Clinical Virology, 14, no. 2 (1999):119-131,
https://doi.org/10.1016/S1386-6532(99)00048-7 . .

TY  - JOUR
AU  - Peco-Antić, A.
AU  - Popović-Rolović, M.
AU  - Gligić, Ana
AU  - Popović, D.
AU  - Jovanović, O.
AU  - Kostić, M.
PY  - 1992
UR  - http://intor.torlakinstitut.com/handle/123456789/52
AB  - From January 1988 to September 1989, seven patients (4 girls and 3 boys, aged 3-12 years) with haemorrhagic fever with renal syndrome (HFRS) were hospitalised at the University Children's Hospital in Belgrade. In four patients the disease appeared as a family outbreak, the others were sporadic cases. In six patients the clinical presentation was suggestive of HFRS, as they had fever with headache, myalgia, sore throat and gastrointestinal illness followed by renal abnormalities. However, severe haemorrhagic syndrome with petechia, haematoma, haematemesis and melaena was present in one patient only. Renal disease presented as nephritic syndrome and/or acute renal failure. Five patients recovered after 2-3 weeks without sequellae, one patient had decreased renal function 17 months after the start of the disease and the remaining patient died. In six patients the diagnosis of HFRS was confirmed serologically by a significant rise in antibody titres against hantaviruses, while in the patient with the fatal and fulminant course of the disease, the diagnosis was established on the basis of epidemiological and autopsy findings. We suggest that children living in endemic areas who develop an ill-defined, febrile and gastrointestinal disease with renal dysfunction should be evaluated for HFRS.
PB  - Springer-Verlag
T2  - Pediatric Nephrology
T1  - Clinical characteristics of haemorrhagic fever with renal syndrome in children
EP  - 338
IS  - 4
SP  - 335
VL  - 6
DO  - 10.1007/BF00869727
ER  - 

@article{
author = "Peco-Antić, A. and Popović-Rolović, M. and Gligić, Ana and Popović, D. and Jovanović, O. and Kostić, M.",
year = "1992",
abstract = "From January 1988 to September 1989, seven patients (4 girls and 3 boys, aged 3-12 years) with haemorrhagic fever with renal syndrome (HFRS) were hospitalised at the University Children's Hospital in Belgrade. In four patients the disease appeared as a family outbreak, the others were sporadic cases. In six patients the clinical presentation was suggestive of HFRS, as they had fever with headache, myalgia, sore throat and gastrointestinal illness followed by renal abnormalities. However, severe haemorrhagic syndrome with petechia, haematoma, haematemesis and melaena was present in one patient only. Renal disease presented as nephritic syndrome and/or acute renal failure. Five patients recovered after 2-3 weeks without sequellae, one patient had decreased renal function 17 months after the start of the disease and the remaining patient died. In six patients the diagnosis of HFRS was confirmed serologically by a significant rise in antibody titres against hantaviruses, while in the patient with the fatal and fulminant course of the disease, the diagnosis was established on the basis of epidemiological and autopsy findings. We suggest that children living in endemic areas who develop an ill-defined, febrile and gastrointestinal disease with renal dysfunction should be evaluated for HFRS.",
publisher = "Springer-Verlag",
journal = "Pediatric Nephrology",
title = "Clinical characteristics of haemorrhagic fever with renal syndrome in children",
pages = "338-335",
number = "4",
volume = "6",
doi = "10.1007/BF00869727"
}

Peco-Antić, A., Popović-Rolović, M., Gligić, A., Popović, D., Jovanović, O.,& Kostić, M.. (1992). Clinical characteristics of haemorrhagic fever with renal syndrome in children. in Pediatric Nephrology
Springer-Verlag., 6(4), 335-338.
https://doi.org/10.1007/BF00869727

Peco-Antić A, Popović-Rolović M, Gligić A, Popović D, Jovanović O, Kostić M. Clinical characteristics of haemorrhagic fever with renal syndrome in children. in Pediatric Nephrology. 1992;6(4):335-338.
doi:10.1007/BF00869727 .

Peco-Antić, A., Popović-Rolović, M., Gligić, Ana, Popović, D., Jovanović, O., Kostić, M., "Clinical characteristics of haemorrhagic fever with renal syndrome in children" in Pediatric Nephrology, 6, no. 4 (1992):335-338,
https://doi.org/10.1007/BF00869727 . .