TY  - DATA
AU  - Patiño-Guillén, Gerardo
AU  - Pešović, Jovan
AU  - Panić, Marko
AU  - Savić-Pavićević, Dušanka
AU  - Bošković, Filip
AU  - Keyser, Ulrich Felix
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/866
AB  - This PDF file includes: → Supplementary Figures 1 to 29 → Supplementary Tables 1 to 8
PB  - Nature
T2  - Nature Communications
T1  - Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8.
IS  - 1
VL  - 15
DO  - 10.17863/CAM.104528
ER  - 

@misc{
author = "Patiño-Guillén, Gerardo and Pešović, Jovan and Panić, Marko and Savić-Pavićević, Dušanka and Bošković, Filip and Keyser, Ulrich Felix",
year = "2024",
abstract = "This PDF file includes: → Supplementary Figures 1 to 29 → Supplementary Tables 1 to 8",
publisher = "Nature",
journal = "Nature Communications",
title = "Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8.",
number = "1",
volume = "15",
doi = "10.17863/CAM.104528"
}

Patiño-Guillén, G., Pešović, J., Panić, M., Savić-Pavićević, D., Bošković, F.,& Keyser, U. F.. (2024). Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8.. in Nature Communications
Nature., 15(1).
https://doi.org/10.17863/CAM.104528

Patiño-Guillén G, Pešović J, Panić M, Savić-Pavićević D, Bošković F, Keyser UF. Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8.. in Nature Communications. 2024;15(1).
doi:10.17863/CAM.104528 .

Patiño-Guillén, Gerardo, Pešović, Jovan, Panić, Marko, Savić-Pavićević, Dušanka, Bošković, Filip, Keyser, Ulrich Felix, "Supplementary Material for: Patiño-Guillén, G.; Pešović, J.; Panić, M.; Savić-Pavićević, D.; Bošković, F.; Keyser, U. F. Single-Molecule RNA Sizing Enables Quantitative Analysis of Alternative Transcription Termination. Nat Commun 2024, 15 (1), 1699. https://doi.org/10.1038/s41467-024-45968-8." in Nature Communications, 15, no. 1 (2024),
https://doi.org/10.17863/CAM.104528 . .

TY  - JOUR
AU  - Patiño-Guillén, Gerardo
AU  - Pešović, Jovan
AU  - Panić, Marko
AU  - Savić-Pavićević, Dušanka
AU  - Bošković, Filip
AU  - Keyser, Ulrich Felix
PY  - 2024
UR  - http://intor.torlakinstitut.com/handle/123456789/865
AB  - Transcription, a critical process in molecular biology, has found many applications in RNA synthesis, including mRNA vaccines and RNA therapeutics. However, current RNA characterization technologies suffer from amplification and enzymatic biases that lead to loss of native information. Here, we introduce a strategy to quantitatively study both transcription and RNA polymerase behaviour by sizing RNA with RNA nanotechnology and nanopores. To begin, we utilize T7 RNA polymerase to transcribe linear DNA lacking termination sequences. Surprisingly, we discover alternative transcription termination in the origin of replication sequence. Next, we employ circular DNA without transcription terminators to perform rolling circle transcription. This allows us to gain valuable insights into the processivity and transcription behaviour of RNA polymerase at the single-molecule level. Our work demonstrates how RNA nanotechnology and nanopores may be used in tandem for the direct and quantitative analysis of RNA transcripts. This methodology provides a promising pathway for accurate RNA structural mapping by enabling the study of full-length RNA transcripts at the single-molecule level.
PB  - Nature
T2  - Nature Communications
T1  - Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination
IS  - 1
SP  - 1699
VL  - 15
DO  - 10.1038/s41467-024-45968-8
ER  - 

@article{
author = "Patiño-Guillén, Gerardo and Pešović, Jovan and Panić, Marko and Savić-Pavićević, Dušanka and Bošković, Filip and Keyser, Ulrich Felix",
year = "2024",
abstract = "Transcription, a critical process in molecular biology, has found many applications in RNA synthesis, including mRNA vaccines and RNA therapeutics. However, current RNA characterization technologies suffer from amplification and enzymatic biases that lead to loss of native information. Here, we introduce a strategy to quantitatively study both transcription and RNA polymerase behaviour by sizing RNA with RNA nanotechnology and nanopores. To begin, we utilize T7 RNA polymerase to transcribe linear DNA lacking termination sequences. Surprisingly, we discover alternative transcription termination in the origin of replication sequence. Next, we employ circular DNA without transcription terminators to perform rolling circle transcription. This allows us to gain valuable insights into the processivity and transcription behaviour of RNA polymerase at the single-molecule level. Our work demonstrates how RNA nanotechnology and nanopores may be used in tandem for the direct and quantitative analysis of RNA transcripts. This methodology provides a promising pathway for accurate RNA structural mapping by enabling the study of full-length RNA transcripts at the single-molecule level.",
publisher = "Nature",
journal = "Nature Communications",
title = "Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination",
number = "1",
pages = "1699",
volume = "15",
doi = "10.1038/s41467-024-45968-8"
}

Patiño-Guillén, G., Pešović, J., Panić, M., Savić-Pavićević, D., Bošković, F.,& Keyser, U. F.. (2024). Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination. in Nature Communications
Nature., 15(1), 1699.
https://doi.org/10.1038/s41467-024-45968-8

Patiño-Guillén G, Pešović J, Panić M, Savić-Pavićević D, Bošković F, Keyser UF. Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination. in Nature Communications. 2024;15(1):1699.
doi:10.1038/s41467-024-45968-8 .

Patiño-Guillén, Gerardo, Pešović, Jovan, Panić, Marko, Savić-Pavićević, Dušanka, Bošković, Filip, Keyser, Ulrich Felix, "Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination" in Nature Communications, 15, no. 1 (2024):1699,
https://doi.org/10.1038/s41467-024-45968-8 . .

TY  - JOUR
AU  - Kecmanović, Miljana
AU  - Dobričić, Valerija
AU  - Dimitrijević, Rajna
AU  - Keckarević, Dušan
AU  - Savić-Pavićević, Dušanka
AU  - Keckarević-Marković, Milica
AU  - Ivković, Maja
AU  - Romac, Stanka
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/312
AB  - Apolipoprotein E (APOE) gene variants are associated with alterations in brain function and increased risk of Alzheimer's disease (AD) and conflicting results have been reported in schizophrenia. Our results showed no significant differences in APOE allele or genotype frequencies between the Serbian schizophrenic patients and control individuals. However, we observed a possible association between particular subtypes of schizophrenia and APOE epsilon 3/epsilon 3 genotype (p = .01221) and epsilon 4 allele showed a tendency toward positive association with responding to typical neuroleptics. APOE genotypes have no major influence on risk of schizophrenia, treatment and response to conventional antipsychotics, and age of onset in schizophrenia.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Schizophrenia and Apolipoprotein E Gene Polymorphism in Serbian Population
EP  - 506
IS  - 7
SP  - 502
VL  - 120
DO  - 10.3109/00207451003765956
ER  - 

@article{
author = "Kecmanović, Miljana and Dobričić, Valerija and Dimitrijević, Rajna and Keckarević, Dušan and Savić-Pavićević, Dušanka and Keckarević-Marković, Milica and Ivković, Maja and Romac, Stanka",
year = "2010",
abstract = "Apolipoprotein E (APOE) gene variants are associated with alterations in brain function and increased risk of Alzheimer's disease (AD) and conflicting results have been reported in schizophrenia. Our results showed no significant differences in APOE allele or genotype frequencies between the Serbian schizophrenic patients and control individuals. However, we observed a possible association between particular subtypes of schizophrenia and APOE epsilon 3/epsilon 3 genotype (p = .01221) and epsilon 4 allele showed a tendency toward positive association with responding to typical neuroleptics. APOE genotypes have no major influence on risk of schizophrenia, treatment and response to conventional antipsychotics, and age of onset in schizophrenia.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Schizophrenia and Apolipoprotein E Gene Polymorphism in Serbian Population",
pages = "506-502",
number = "7",
volume = "120",
doi = "10.3109/00207451003765956"
}

Kecmanović, M., Dobričić, V., Dimitrijević, R., Keckarević, D., Savić-Pavićević, D., Keckarević-Marković, M., Ivković, M.,& Romac, S.. (2010). Schizophrenia and Apolipoprotein E Gene Polymorphism in Serbian Population. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 120(7), 502-506.
https://doi.org/10.3109/00207451003765956

Kecmanović M, Dobričić V, Dimitrijević R, Keckarević D, Savić-Pavićević D, Keckarević-Marković M, Ivković M, Romac S. Schizophrenia and Apolipoprotein E Gene Polymorphism in Serbian Population. in International Journal of Neuroscience. 2010;120(7):502-506.
doi:10.3109/00207451003765956 .

Kecmanović, Miljana, Dobričić, Valerija, Dimitrijević, Rajna, Keckarević, Dušan, Savić-Pavićević, Dušanka, Keckarević-Marković, Milica, Ivković, Maja, Romac, Stanka, "Schizophrenia and Apolipoprotein E Gene Polymorphism in Serbian Population" in International Journal of Neuroscience, 120, no. 7 (2010):502-506,
https://doi.org/10.3109/00207451003765956 . .

TY  - JOUR
AU  - Dimitrijević, Rajna
AU  - Cadez, Ivana
AU  - Keckarević-Marković, Milica
AU  - Keckarević, Dušan
AU  - Kecmanović, Miljana
AU  - Dobričić, Valerija
AU  - Savić-Pavićević, Dušanka
AU  - Brajusković, Goran
AU  - Romac, Stanka
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/299
AB  - Prion diseases are a group of etiologically heterogenous neurodegenerative disorders. We have analyzed the coding region of PRNP gene in 121 healthy citizens of Serbia to determine whether the frequencies of M129V, E219K, and octapeptide repeat number polymorphism. For Serbian population, polymorphism of PRNP gene at codon 129 does not differ from healthy European populations. Also codon 219 is monomorphic for the Glu allele both in Serbian population and other European populations. On the contrary, in Serbian population we did not detect any deletions or insertions in octapeptide repeat region, whereas deletions were detected in other European populations.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Polymorphisms of the Prion Protein Gene (PRNP) in a Serbian Population
EP  - 501
IS  - 7
SP  - 496
VL  - 120
DO  - 10.3109/00207451003765907
ER  - 

@article{
author = "Dimitrijević, Rajna and Cadez, Ivana and Keckarević-Marković, Milica and Keckarević, Dušan and Kecmanović, Miljana and Dobričić, Valerija and Savić-Pavićević, Dušanka and Brajusković, Goran and Romac, Stanka",
year = "2010",
abstract = "Prion diseases are a group of etiologically heterogenous neurodegenerative disorders. We have analyzed the coding region of PRNP gene in 121 healthy citizens of Serbia to determine whether the frequencies of M129V, E219K, and octapeptide repeat number polymorphism. For Serbian population, polymorphism of PRNP gene at codon 129 does not differ from healthy European populations. Also codon 219 is monomorphic for the Glu allele both in Serbian population and other European populations. On the contrary, in Serbian population we did not detect any deletions or insertions in octapeptide repeat region, whereas deletions were detected in other European populations.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Polymorphisms of the Prion Protein Gene (PRNP) in a Serbian Population",
pages = "501-496",
number = "7",
volume = "120",
doi = "10.3109/00207451003765907"
}

Dimitrijević, R., Cadez, I., Keckarević-Marković, M., Keckarević, D., Kecmanović, M., Dobričić, V., Savić-Pavićević, D., Brajusković, G.,& Romac, S.. (2010). Polymorphisms of the Prion Protein Gene (PRNP) in a Serbian Population. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 120(7), 496-501.
https://doi.org/10.3109/00207451003765907

Dimitrijević R, Cadez I, Keckarević-Marković M, Keckarević D, Kecmanović M, Dobričić V, Savić-Pavićević D, Brajusković G, Romac S. Polymorphisms of the Prion Protein Gene (PRNP) in a Serbian Population. in International Journal of Neuroscience. 2010;120(7):496-501.
doi:10.3109/00207451003765907 .

Dimitrijević, Rajna, Cadez, Ivana, Keckarević-Marković, Milica, Keckarević, Dušan, Kecmanović, Miljana, Dobričić, Valerija, Savić-Pavićević, Dušanka, Brajusković, Goran, Romac, Stanka, "Polymorphisms of the Prion Protein Gene (PRNP) in a Serbian Population" in International Journal of Neuroscience, 120, no. 7 (2010):496-501,
https://doi.org/10.3109/00207451003765907 . .

TY  - JOUR
AU  - Janković, N.
AU  - Kemanović, M.
AU  - Dimitrijević, Rajna
AU  - Keckarević-Marković, Milica
AU  - Dobričić, Valerija
AU  - Savić-Pavićević, Dušanka
AU  - Romac, Stanka
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/251
AB  - Saitohin (STH) is located in the intron of the human gene for microtubule-associated protein tau. Q7R polymorphism has been identified in the STH gene. Some neurodegenerative disorders were found to be associated with the presence of certain STH allele. This study genotyped 37 subjects with diagnosis of Huntington's disease, but lacking mutations in HD, PRNP, JPH-3, and FTL genes for STH polymorphism. It was determined that Q allele of STH gene was over-represented in a tested group of patients (P  gt  Pt). Over-representation of Q allele in a group of patients might be considered as genetic risk factor for HD like diseases.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - HD phenocopies - Possible role of saitohin gene
EP  - 397
IS  - 3
SP  - 391
VL  - 118
DO  - 10.1080/00207450701593103
ER  - 

@article{
author = "Janković, N. and Kemanović, M. and Dimitrijević, Rajna and Keckarević-Marković, Milica and Dobričić, Valerija and Savić-Pavićević, Dušanka and Romac, Stanka",
year = "2008",
abstract = "Saitohin (STH) is located in the intron of the human gene for microtubule-associated protein tau. Q7R polymorphism has been identified in the STH gene. Some neurodegenerative disorders were found to be associated with the presence of certain STH allele. This study genotyped 37 subjects with diagnosis of Huntington's disease, but lacking mutations in HD, PRNP, JPH-3, and FTL genes for STH polymorphism. It was determined that Q allele of STH gene was over-represented in a tested group of patients (P  gt  Pt). Over-representation of Q allele in a group of patients might be considered as genetic risk factor for HD like diseases.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "HD phenocopies - Possible role of saitohin gene",
pages = "397-391",
number = "3",
volume = "118",
doi = "10.1080/00207450701593103"
}

Janković, N., Kemanović, M., Dimitrijević, R., Keckarević-Marković, M., Dobričić, V., Savić-Pavićević, D.,& Romac, S.. (2008). HD phenocopies - Possible role of saitohin gene. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 118(3), 391-397.
https://doi.org/10.1080/00207450701593103

Janković N, Kemanović M, Dimitrijević R, Keckarević-Marković M, Dobričić V, Savić-Pavićević D, Romac S. HD phenocopies - Possible role of saitohin gene. in International Journal of Neuroscience. 2008;118(3):391-397.
doi:10.1080/00207450701593103 .

Janković, N., Kemanović, M., Dimitrijević, Rajna, Keckarević-Marković, Milica, Dobričić, Valerija, Savić-Pavićević, Dušanka, Romac, Stanka, "HD phenocopies - Possible role of saitohin gene" in International Journal of Neuroscience, 118, no. 3 (2008):391-397,
https://doi.org/10.1080/00207450701593103 . .