TY  - JOUR
AU  - Leposavić, Gordana
AU  - Karapetrović, B.
AU  - Obradović, S.
AU  - Danković, B.V.
AU  - Kosec, Duško
PY  - 1996
UR  - http://intor.torlakinstitut.com/handle/123456789/61
AB  - As an organ responsible for generation of T-cell repertoire the thymus occupies a central position in establishment of mature immune response. To assess the potential role of the gonadal steroids in development and maintenance of immunological sexual dimorphism, the effects of gonadectomy pre- and postpuberty on the thymocyte profile of male and female rats were examined. Rats aged 30 days or 75 days were gonadectomized; 30 days later the thymic cellularity was estimated and the expression of the cell surface antigens (CD4 and CD8) and the T-cell receptor (TCR) alpha beta was analyzed by now cytometry. Regardless of age at surgery, the thymus weight and total thymocyte yield were greater in sham-operated males than females; this sexual dimorphism in thymic cellularity persisted after gonadectomy. Sexual dimorphism in the composition of thymocyte subsets was also evident in sham-operated rats, with males expressing a higher percentage of CD4-8- cells, and remained after gonadectomy of adult rats. In male rats, gonadectomy at day 75 increased the percentage of CD4+8- single-positive and TCR alpha beta + cells. In contrast, in females, ovariectomy decreased the percentages of CD4+8- single-positive, CD4-CD8- double-negative, and TCR alpha beta + cells and increased the percentage of CD4+CD8+ double-positive cells. In the immature rats gonadectomy increased the percentages of CD4+8- single-positive and TCR alpha beta + thymocytes and decreased the percentages of double-positive and double-negative cells in males, while in the female it increased the percentage of CD4+8- single-positive thymocytes. Gonadectomy at that age abolished the sexual dimorphism in the expression of accessory molecules (i.e., CD4/CD8), but facilitated gender-specific expression of TCR alpha beta. In conclusion, the results suggest that the gonadal steroids are more important for the development than for the maintenance of the sexual dimorphism in the thymocyte composition.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Pharmacology Biochemistry and Behavior
T1  - Differential effects of gonadectomy on the thymocyte phenotypic profile in male and female rats
EP  - 276
IS  - 1
SP  - 269
VL  - 54
DO  - 10.1016/0091-3057(95)02165-5
ER  - 

@article{
author = "Leposavić, Gordana and Karapetrović, B. and Obradović, S. and Danković, B.V. and Kosec, Duško",
year = "1996",
abstract = "As an organ responsible for generation of T-cell repertoire the thymus occupies a central position in establishment of mature immune response. To assess the potential role of the gonadal steroids in development and maintenance of immunological sexual dimorphism, the effects of gonadectomy pre- and postpuberty on the thymocyte profile of male and female rats were examined. Rats aged 30 days or 75 days were gonadectomized; 30 days later the thymic cellularity was estimated and the expression of the cell surface antigens (CD4 and CD8) and the T-cell receptor (TCR) alpha beta was analyzed by now cytometry. Regardless of age at surgery, the thymus weight and total thymocyte yield were greater in sham-operated males than females; this sexual dimorphism in thymic cellularity persisted after gonadectomy. Sexual dimorphism in the composition of thymocyte subsets was also evident in sham-operated rats, with males expressing a higher percentage of CD4-8- cells, and remained after gonadectomy of adult rats. In male rats, gonadectomy at day 75 increased the percentage of CD4+8- single-positive and TCR alpha beta + cells. In contrast, in females, ovariectomy decreased the percentages of CD4+8- single-positive, CD4-CD8- double-negative, and TCR alpha beta + cells and increased the percentage of CD4+CD8+ double-positive cells. In the immature rats gonadectomy increased the percentages of CD4+8- single-positive and TCR alpha beta + thymocytes and decreased the percentages of double-positive and double-negative cells in males, while in the female it increased the percentage of CD4+8- single-positive thymocytes. Gonadectomy at that age abolished the sexual dimorphism in the expression of accessory molecules (i.e., CD4/CD8), but facilitated gender-specific expression of TCR alpha beta. In conclusion, the results suggest that the gonadal steroids are more important for the development than for the maintenance of the sexual dimorphism in the thymocyte composition.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Pharmacology Biochemistry and Behavior",
title = "Differential effects of gonadectomy on the thymocyte phenotypic profile in male and female rats",
pages = "276-269",
number = "1",
volume = "54",
doi = "10.1016/0091-3057(95)02165-5"
}

Leposavić, G., Karapetrović, B., Obradović, S., Danković, B.V.,& Kosec, D.. (1996). Differential effects of gonadectomy on the thymocyte phenotypic profile in male and female rats. in Pharmacology Biochemistry and Behavior
Pergamon-Elsevier Science Ltd, Oxford., 54(1), 269-276.
https://doi.org/10.1016/0091-3057(95)02165-5

Leposavić G, Karapetrović B, Obradović S, Danković B, Kosec D. Differential effects of gonadectomy on the thymocyte phenotypic profile in male and female rats. in Pharmacology Biochemistry and Behavior. 1996;54(1):269-276.
doi:10.1016/0091-3057(95)02165-5 .

Leposavić, Gordana, Karapetrović, B., Obradović, S., Danković, B.V., Kosec, Duško, "Differential effects of gonadectomy on the thymocyte phenotypic profile in male and female rats" in Pharmacology Biochemistry and Behavior, 54, no. 1 (1996):269-276,
https://doi.org/10.1016/0091-3057(95)02165-5 . .

TY  - JOUR
AU  - Karapetrović, B.
AU  - Kosec, Duško
AU  - Leposavić, Gordana
PY  - 1996
UR  - http://intor.torlakinstitut.com/handle/123456789/60
AB  - In order to assess the influence of gonadal hormones on the process of intrathymic T cell-maturation, peripubertal(30 days old) and young adult (60 days old) rats were castrated and the total thymic cellularity as well as the cellularity of the main thymocyte subpopulations defined by the expression of CD4 and CD8 molecules, and TCRab were analyzed in adult (75 days old) rats. The total yield of thymocytes was significantly increased in adults castrated at day 30 over the controls. Also, the total number of CD4+/CD8+ double positive (DP) thymocytes as well as the total number of both the CD4+/CD8- and CD4-/CD8+ single positive (SP) and TCR alpha beta positive thymocytes were significantly increased in these rats. However, castration performed in young adult rats affected neither the total thymic cellularity nor the cellularity of the main thymocyte subpopulations. Results clearly indicate that: a) castration performed before sexual maturity affects the process of intrathymic T cell maturation in adults and b) the process of intrathymic T cell development is not altered in adults of the same age castrated in very early adulthood.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Differential effects of castration in sexually immature and mature rats on the phenotypic profile of thymocytes
EP  - 275
IS  - 5-6
SP  - 271
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_intor_60
ER  - 

@article{
author = "Karapetrović, B. and Kosec, Duško and Leposavić, Gordana",
year = "1996",
abstract = "In order to assess the influence of gonadal hormones on the process of intrathymic T cell-maturation, peripubertal(30 days old) and young adult (60 days old) rats were castrated and the total thymic cellularity as well as the cellularity of the main thymocyte subpopulations defined by the expression of CD4 and CD8 molecules, and TCRab were analyzed in adult (75 days old) rats. The total yield of thymocytes was significantly increased in adults castrated at day 30 over the controls. Also, the total number of CD4+/CD8+ double positive (DP) thymocytes as well as the total number of both the CD4+/CD8- and CD4-/CD8+ single positive (SP) and TCR alpha beta positive thymocytes were significantly increased in these rats. However, castration performed in young adult rats affected neither the total thymic cellularity nor the cellularity of the main thymocyte subpopulations. Results clearly indicate that: a) castration performed before sexual maturity affects the process of intrathymic T cell maturation in adults and b) the process of intrathymic T cell development is not altered in adults of the same age castrated in very early adulthood.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Differential effects of castration in sexually immature and mature rats on the phenotypic profile of thymocytes",
pages = "275-271",
number = "5-6",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_intor_60"
}

Karapetrović, B., Kosec, D.,& Leposavić, G.. (1996). Differential effects of castration in sexually immature and mature rats on the phenotypic profile of thymocytes. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 46(5-6), 271-275.
https://hdl.handle.net/21.15107/rcub_intor_60

Karapetrović B, Kosec D, Leposavić G. Differential effects of castration in sexually immature and mature rats on the phenotypic profile of thymocytes. in Acta veterinaria - Beograd. 1996;46(5-6):271-275.
https://hdl.handle.net/21.15107/rcub_intor_60 .

Karapetrović, B., Kosec, Duško, Leposavić, Gordana, "Differential effects of castration in sexually immature and mature rats on the phenotypic profile of thymocytes" in Acta veterinaria - Beograd, 46, no. 5-6 (1996):271-275,
https://hdl.handle.net/21.15107/rcub_intor_60 .