Schuerer, Nadine

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orcid::0000-0002-5894-2784
  • Schuerer, Nadine (10)
Projects

Author's Bibliography

Effects of chitosan and chitosan Nacetylcysteine solutions on conjunctival epithelial cells

Schuerer, Nadine; Stein, Elisabeth; Inić-Kanada, Aleksandra; Ghasemian, Ehsan; Stojanović, Marijana; Montanaro, Jacqueline; Bintner, Nora; Hohenadl, Christine; Sachsenhofer, Robert; Barisani-Asenbauer, Talin

(2018)

TY  - JOUR
AU  - Schuerer, Nadine
AU  - Stein, Elisabeth
AU  - Inić-Kanada, Aleksandra
AU  - Ghasemian, Ehsan
AU  - Stojanović, Marijana
AU  - Montanaro, Jacqueline
AU  - Bintner, Nora
AU  - Hohenadl, Christine
AU  - Sachsenhofer, Robert
AU  - Barisani-Asenbauer, Talin
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/506
T2  - Journal of EuCornea
T1  - Effects of chitosan and chitosan Nacetylcysteine solutions on conjunctival epithelial cells
EP  - 18
SP  - 12
VL  - 1
UR  - conv_666
ER  - 
@article{
author = "Schuerer, Nadine and Stein, Elisabeth and Inić-Kanada, Aleksandra and Ghasemian, Ehsan and Stojanović, Marijana and Montanaro, Jacqueline and Bintner, Nora and Hohenadl, Christine and Sachsenhofer, Robert and Barisani-Asenbauer, Talin",
year = "2018",
journal = "Journal of EuCornea",
title = "Effects of chitosan and chitosan Nacetylcysteine solutions on conjunctival epithelial cells",
pages = "18-12",
volume = "1",
url = "conv_666"
}
Schuerer, N., Stein, E., Inić-Kanada, A., Ghasemian, E., Stojanović, M., Montanaro, J., Bintner, N., Hohenadl, C., Sachsenhofer, R.,& Barisani-Asenbauer, T.. (2018). Effects of chitosan and chitosan Nacetylcysteine solutions on conjunctival epithelial cells. in Journal of EuCornea, 1, 12-18.
conv_666
Schuerer N, Stein E, Inić-Kanada A, Ghasemian E, Stojanović M, Montanaro J, Bintner N, Hohenadl C, Sachsenhofer R, Barisani-Asenbauer T. Effects of chitosan and chitosan Nacetylcysteine solutions on conjunctival epithelial cells. in Journal of EuCornea. 2018;1:12-18.
conv_666 .
Schuerer, Nadine, Stein, Elisabeth, Inić-Kanada, Aleksandra, Ghasemian, Ehsan, Stojanović, Marijana, Montanaro, Jacqueline, Bintner, Nora, Hohenadl, Christine, Sachsenhofer, Robert, Barisani-Asenbauer, Talin, "Effects of chitosan and chitosan Nacetylcysteine solutions on conjunctival epithelial cells" in Journal of EuCornea, 1 (2018):12-18,
conv_666 .

Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo

Inić-Kanada, Aleksandra; Stein, Elisabeth; Stojanović, Marijana; Schuerer, Nadine; Ghasemian, Ehsan; Filipović, Ana; Marinković, Emilija; Kosanović, Dejana; Barisani-Asenbauer, Talin

(Springer, Dordrecht, 2018)

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stein, Elisabeth
AU  - Stojanović, Marijana
AU  - Schuerer, Nadine
AU  - Ghasemian, Ehsan
AU  - Filipović, Ana
AU  - Marinković, Emilija
AU  - Kosanović, Dejana
AU  - Barisani-Asenbauer, Talin
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/502
AB  - Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.
PB  - Springer, Dordrecht
T2  - Journal of Applied Phycology
T1  - Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo
EP  - 2610
IS  - 4
SP  - 2601
VL  - 30
DO  - 10.1007/s10811-018-1435-0
UR  - conv_438
ER  - 
@article{
author = "Inić-Kanada, Aleksandra and Stein, Elisabeth and Stojanović, Marijana and Schuerer, Nadine and Ghasemian, Ehsan and Filipović, Ana and Marinković, Emilija and Kosanović, Dejana and Barisani-Asenbauer, Talin",
year = "2018",
abstract = "Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.",
publisher = "Springer, Dordrecht",
journal = "Journal of Applied Phycology",
title = "Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo",
pages = "2610-2601",
number = "4",
volume = "30",
doi = "10.1007/s10811-018-1435-0",
url = "conv_438"
}
Inić-Kanada, A., Stein, E., Stojanović, M., Schuerer, N., Ghasemian, E., Filipović, A., Marinković, E., Kosanović, D.,& Barisani-Asenbauer, T.. (2018). Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo. in Journal of Applied Phycology
Springer, Dordrecht., 30(4), 2601-2610.
https://doi.org/10.1007/s10811-018-1435-0
conv_438
Inić-Kanada A, Stein E, Stojanović M, Schuerer N, Ghasemian E, Filipović A, Marinković E, Kosanović D, Barisani-Asenbauer T. Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo. in Journal of Applied Phycology. 2018;30(4):2601-2610.
doi:10.1007/s10811-018-1435-0
conv_438 .
Inić-Kanada, Aleksandra, Stein, Elisabeth, Stojanović, Marijana, Schuerer, Nadine, Ghasemian, Ehsan, Filipović, Ana, Marinković, Emilija, Kosanović, Dejana, Barisani-Asenbauer, Talin, "Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo" in Journal of Applied Phycology, 30, no. 4 (2018):2601-2610,
https://doi.org/10.1007/s10811-018-1435-0 .,
conv_438 .
4
14
11
13

The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection

Filipović, Ana; Ghasemian, Ehsan; Inić-Kanada, Aleksandra; Lukić, Ivana; Stein, Elisabeth; Marinković, Emilija; Đokić, Radmila; Kosanović, Dejana; Schuerer, Nadine; Chalabi, Hadeel; Belij-Rammerstorfer, Sandra; Stojanović, Marijana; Barisani-Asenbauer, Talin

(Public Library Science, San Francisco, 2017)

TY  - JOUR
AU  - Filipović, Ana
AU  - Ghasemian, Ehsan
AU  - Inić-Kanada, Aleksandra
AU  - Lukić, Ivana
AU  - Stein, Elisabeth
AU  - Marinković, Emilija
AU  - Đokić, Radmila
AU  - Kosanović, Dejana
AU  - Schuerer, Nadine
AU  - Chalabi, Hadeel
AU  - Belij-Rammerstorfer, Sandra
AU  - Stojanović, Marijana
AU  - Barisani-Asenbauer, Talin
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/477
AB  - Following infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1x10(2), 1x10(4), and 1x10(6) inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4(+) and CD8(+) cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4(+) and CD8(+) cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4(+) and CD8(+) cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1x10(4), and 1x10(6) IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection
IS  - 7
VL  - 12
DO  - 10.1371/journal.pone.0180551
UR  - conv_416
ER  - 
@article{
author = "Filipović, Ana and Ghasemian, Ehsan and Inić-Kanada, Aleksandra and Lukić, Ivana and Stein, Elisabeth and Marinković, Emilija and Đokić, Radmila and Kosanović, Dejana and Schuerer, Nadine and Chalabi, Hadeel and Belij-Rammerstorfer, Sandra and Stojanović, Marijana and Barisani-Asenbauer, Talin",
year = "2017",
abstract = "Following infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1x10(2), 1x10(4), and 1x10(6) inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4(+) and CD8(+) cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4(+) and CD8(+) cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4(+) and CD8(+) cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1x10(4), and 1x10(6) IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection",
number = "7",
volume = "12",
doi = "10.1371/journal.pone.0180551",
url = "conv_416"
}
Filipović, A., Ghasemian, E., Inić-Kanada, A., Lukić, I., Stein, E., Marinković, E., Đokić, R., Kosanović, D., Schuerer, N., Chalabi, H., Belij-Rammerstorfer, S., Stojanović, M.,& Barisani-Asenbauer, T.. (2017). The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection. in PLoS One
Public Library Science, San Francisco., 12(7).
https://doi.org/10.1371/journal.pone.0180551
conv_416
Filipović A, Ghasemian E, Inić-Kanada A, Lukić I, Stein E, Marinković E, Đokić R, Kosanović D, Schuerer N, Chalabi H, Belij-Rammerstorfer S, Stojanović M, Barisani-Asenbauer T. The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection. in PLoS One. 2017;12(7).
doi:10.1371/journal.pone.0180551
conv_416 .
Filipović, Ana, Ghasemian, Ehsan, Inić-Kanada, Aleksandra, Lukić, Ivana, Stein, Elisabeth, Marinković, Emilija, Đokić, Radmila, Kosanović, Dejana, Schuerer, Nadine, Chalabi, Hadeel, Belij-Rammerstorfer, Sandra, Stojanović, Marijana, Barisani-Asenbauer, Talin, "The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection" in PLoS One, 12, no. 7 (2017),
https://doi.org/10.1371/journal.pone.0180551 .,
conv_416 .
1
7
8
8

Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.

Inić-Kanada, Aleksandra; Lukić, Ivana; Stojanović, Marijana; Stein, Elisabeth; Marinković, Emilija; Filipović, Ana; Đokić, Radmila; Kosanović, Dejana; Schuerer, Nadine; Ghasemian, Ehsan; Barisani-Asenbauer, Talin

(Assoc Research Vision Ophthalmology Inc, Rockville, 2017)

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Lukić, Ivana
AU  - Stojanović, Marijana
AU  - Stein, Elisabeth
AU  - Marinković, Emilija
AU  - Filipović, Ana
AU  - Đokić, Radmila
AU  - Kosanović, Dejana
AU  - Schuerer, Nadine
AU  - Ghasemian, Ehsan
AU  - Barisani-Asenbauer, Talin
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/486
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.
IS  - 8
VL  - 58
UR  - conv_432
ER  - 
@conference{
author = "Inić-Kanada, Aleksandra and Lukić, Ivana and Stojanović, Marijana and Stein, Elisabeth and Marinković, Emilija and Filipović, Ana and Đokić, Radmila and Kosanović, Dejana and Schuerer, Nadine and Ghasemian, Ehsan and Barisani-Asenbauer, Talin",
year = "2017",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.",
number = "8",
volume = "58",
url = "conv_432"
}
Inić-Kanada, A., Lukić, I., Stojanović, M., Stein, E., Marinković, E., Filipović, A., Đokić, R., Kosanović, D., Schuerer, N., Ghasemian, E.,& Barisani-Asenbauer, T.. (2017). Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 58(8).
conv_432
Inić-Kanada A, Lukić I, Stojanović M, Stein E, Marinković E, Filipović A, Đokić R, Kosanović D, Schuerer N, Ghasemian E, Barisani-Asenbauer T. Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.. in Investigative Ophthalmology & Visual Science. 2017;58(8).
conv_432 .
Inić-Kanada, Aleksandra, Lukić, Ivana, Stojanović, Marijana, Stein, Elisabeth, Marinković, Emilija, Filipović, Ana, Đokić, Radmila, Kosanović, Dejana, Schuerer, Nadine, Ghasemian, Ehsan, Barisani-Asenbauer, Talin, "Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection." in Investigative Ophthalmology & Visual Science, 58, no. 8 (2017),
conv_432 .

Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection

Belij-Rammerstorfer, Sandra; Inić-Kanada, Aleksandra; Stojanović, Marijana; Marinković, Emilija; Lukić, Ivana; Stein, Elisabeth; Montanaro, Jacqueline; Bintner, Nora; Schuerer, Nadine; Ghasemian, Ehsan; Kundi, Michael; Barisani-Asenbauer, Talin

(Elsevier Science Bv, Amsterdam, 2016)

TY  - JOUR
AU  - Belij-Rammerstorfer, Sandra
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Stein, Elisabeth
AU  - Montanaro, Jacqueline
AU  - Bintner, Nora
AU  - Schuerer, Nadine
AU  - Ghasemian, Ehsan
AU  - Kundi, Michael
AU  - Barisani-Asenbauer, Talin
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/470
AB  - The aim of this study was to determine whether infectious dose of Chlamydia caviae after repeated infections influences the immunological responses and subsequent clearance of pathogen at the ocular surface of guinea pigs. Animals were infected three times via the conjunctiva at six- and twelve-week intervals by applying either 1 x 10(4) or 1 x 10(6) inclusion-forming units (IFUs) of C. caviae. Ocular pathology, infection course, C. caviae-specific serum IgG levels and their capacity to bind and neutralize infection ex vivo were assessed. Animals infected with 1 x 10(4) IFUs had completely diminished ocular infection and pathology after the 2nd infection with increased levels of C. caviae-specific serum IgG and their effective capacity to bind and neutralize C. caviae. Only partial protection was observed in animals infected with 1 x 10(6) IFUs after the 2nd and 3rd infections. Our findings show that full protection was observed in animals repeatedly infected with the lower dose. The lower dose appeared not to compromise the host immune system, thereby enabling fast clearance of the pathogen and the establishment of competent neutralizing antibodies. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Microbes and Infection
T1  - Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection
EP  - 262
IS  - 4
SP  - 254
VL  - 18
DO  - 10.1016/j.micinf.2015.12.001
UR  - conv_380
ER  - 
@article{
author = "Belij-Rammerstorfer, Sandra and Inić-Kanada, Aleksandra and Stojanović, Marijana and Marinković, Emilija and Lukić, Ivana and Stein, Elisabeth and Montanaro, Jacqueline and Bintner, Nora and Schuerer, Nadine and Ghasemian, Ehsan and Kundi, Michael and Barisani-Asenbauer, Talin",
year = "2016",
abstract = "The aim of this study was to determine whether infectious dose of Chlamydia caviae after repeated infections influences the immunological responses and subsequent clearance of pathogen at the ocular surface of guinea pigs. Animals were infected three times via the conjunctiva at six- and twelve-week intervals by applying either 1 x 10(4) or 1 x 10(6) inclusion-forming units (IFUs) of C. caviae. Ocular pathology, infection course, C. caviae-specific serum IgG levels and their capacity to bind and neutralize infection ex vivo were assessed. Animals infected with 1 x 10(4) IFUs had completely diminished ocular infection and pathology after the 2nd infection with increased levels of C. caviae-specific serum IgG and their effective capacity to bind and neutralize C. caviae. Only partial protection was observed in animals infected with 1 x 10(6) IFUs after the 2nd and 3rd infections. Our findings show that full protection was observed in animals repeatedly infected with the lower dose. The lower dose appeared not to compromise the host immune system, thereby enabling fast clearance of the pathogen and the establishment of competent neutralizing antibodies. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Microbes and Infection",
title = "Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection",
pages = "262-254",
number = "4",
volume = "18",
doi = "10.1016/j.micinf.2015.12.001",
url = "conv_380"
}
Belij-Rammerstorfer, S., Inić-Kanada, A., Stojanović, M., Marinković, E., Lukić, I., Stein, E., Montanaro, J., Bintner, N., Schuerer, N., Ghasemian, E., Kundi, M.,& Barisani-Asenbauer, T.. (2016). Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection. in Microbes and Infection
Elsevier Science Bv, Amsterdam., 18(4), 254-262.
https://doi.org/10.1016/j.micinf.2015.12.001
conv_380
Belij-Rammerstorfer S, Inić-Kanada A, Stojanović M, Marinković E, Lukić I, Stein E, Montanaro J, Bintner N, Schuerer N, Ghasemian E, Kundi M, Barisani-Asenbauer T. Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection. in Microbes and Infection. 2016;18(4):254-262.
doi:10.1016/j.micinf.2015.12.001
conv_380 .
Belij-Rammerstorfer, Sandra, Inić-Kanada, Aleksandra, Stojanović, Marijana, Marinković, Emilija, Lukić, Ivana, Stein, Elisabeth, Montanaro, Jacqueline, Bintner, Nora, Schuerer, Nadine, Ghasemian, Ehsan, Kundi, Michael, Barisani-Asenbauer, Talin, "Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection" in Microbes and Infection, 18, no. 4 (2016):254-262,
https://doi.org/10.1016/j.micinf.2015.12.001 .,
conv_380 .
6
7
7
8

A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C

Inić-Kanada, Aleksandra; Stojanović, Marijana; Marinković, Emilija; Becker, Elisabeth; Stein, Elisabeth; Lukić, Ivana; Đokić, Radmila; Schuerer, Nadine; Hegemann, Johannes H.; Barisani-Asenbauer, Talin

(Public Library Science, San Francisco, 2016)

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Becker, Elisabeth
AU  - Stein, Elisabeth
AU  - Lukić, Ivana
AU  - Đokić, Radmila
AU  - Schuerer, Nadine
AU  - Hegemann, Johannes H.
AU  - Barisani-Asenbauer, Talin
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/455
AB  - Recent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving the way for new possibilities to oppose the societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate the risks associated with the use of a whole-cell vaccine. Our rationale for the design of an efficient subunit vaccine against Chlamydia trachomatis (Ct) is based on the membrane proteins involved in the initial Ct-host cell contact and on the route of immunization that mimics the natural infection process (i.e., via the ocular mucosa). The first aim of our study was to characterize the specific conjunctival and vaginal immune responses following eye drop immunization in BALB/c mice, using the N-terminal portion of the Ct serovar E polymorphic membrane protein C (N-PmpC) as the subunit vaccine antigen. Second, we aimed to examine the adjuvant properties of the probiotic Lactobacillus rhamnosus (LB) when formulated with N-PmpC. N-PmpC applied alone stimulated the production of N-PmpC-and Ct serovar B-specific antibodies in serum, tears and vaginal washes, whereas the combination with LB significantly enhanced these responses. The N-PmpC/LB combination initiated a T cell response characterized by an elevated percentage of CD25+ T cells and CD8+ effector T cells, enhanced CD4+ T-helper 1 skewing, and increased regulatory T cell responses. Together, these results show that eye drop vaccination with combined use of N-PmpC and a live probiotic LB stimulates specific cellular and humoral immune responses, not only locally in the conjunctiva but also in the vaginal mucosa, which could be a promising approach in Ct vaccine development.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0157875
UR  - conv_393
ER  - 
@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Marinković, Emilija and Becker, Elisabeth and Stein, Elisabeth and Lukić, Ivana and Đokić, Radmila and Schuerer, Nadine and Hegemann, Johannes H. and Barisani-Asenbauer, Talin",
year = "2016",
abstract = "Recent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving the way for new possibilities to oppose the societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate the risks associated with the use of a whole-cell vaccine. Our rationale for the design of an efficient subunit vaccine against Chlamydia trachomatis (Ct) is based on the membrane proteins involved in the initial Ct-host cell contact and on the route of immunization that mimics the natural infection process (i.e., via the ocular mucosa). The first aim of our study was to characterize the specific conjunctival and vaginal immune responses following eye drop immunization in BALB/c mice, using the N-terminal portion of the Ct serovar E polymorphic membrane protein C (N-PmpC) as the subunit vaccine antigen. Second, we aimed to examine the adjuvant properties of the probiotic Lactobacillus rhamnosus (LB) when formulated with N-PmpC. N-PmpC applied alone stimulated the production of N-PmpC-and Ct serovar B-specific antibodies in serum, tears and vaginal washes, whereas the combination with LB significantly enhanced these responses. The N-PmpC/LB combination initiated a T cell response characterized by an elevated percentage of CD25+ T cells and CD8+ effector T cells, enhanced CD4+ T-helper 1 skewing, and increased regulatory T cell responses. Together, these results show that eye drop vaccination with combined use of N-PmpC and a live probiotic LB stimulates specific cellular and humoral immune responses, not only locally in the conjunctiva but also in the vaginal mucosa, which could be a promising approach in Ct vaccine development.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0157875",
url = "conv_393"
}
Inić-Kanada, A., Stojanović, M., Marinković, E., Becker, E., Stein, E., Lukić, I., Đokić, R., Schuerer, N., Hegemann, J. H.,& Barisani-Asenbauer, T.. (2016). A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C. in PLoS One
Public Library Science, San Francisco., 11(9).
https://doi.org/10.1371/journal.pone.0157875
conv_393
Inić-Kanada A, Stojanović M, Marinković E, Becker E, Stein E, Lukić I, Đokić R, Schuerer N, Hegemann JH, Barisani-Asenbauer T. A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C. in PLoS One. 2016;11(9).
doi:10.1371/journal.pone.0157875
conv_393 .
Inić-Kanada, Aleksandra, Stojanović, Marijana, Marinković, Emilija, Becker, Elisabeth, Stein, Elisabeth, Lukić, Ivana, Đokić, Radmila, Schuerer, Nadine, Hegemann, Johannes H., Barisani-Asenbauer, Talin, "A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C" in PLoS One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0157875 .,
conv_393 .
13
9
12

Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization

Inić-Kanada, Aleksandra; Stojanović, Marijana; Marinković, Emilija; Stein, Elisabeth; Lukić, Ivana; Belij, Sandra; Schuerer, Nadine; Montanaro, Jacqueline; Ghasemian, Ehsan; Đokić, Radmila; Barisani-Asenbauer, Talin

(Assoc Research Vision Ophthalmology Inc, Rockville, 2016)

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stein, Elisabeth
AU  - Lukić, Ivana
AU  - Belij, Sandra
AU  - Schuerer, Nadine
AU  - Montanaro, Jacqueline
AU  - Ghasemian, Ehsan
AU  - Đokić, Radmila
AU  - Barisani-Asenbauer, Talin
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/459
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization
IS  - 12
VL  - 57
UR  - conv_405
ER  - 
@conference{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Marinković, Emilija and Stein, Elisabeth and Lukić, Ivana and Belij, Sandra and Schuerer, Nadine and Montanaro, Jacqueline and Ghasemian, Ehsan and Đokić, Radmila and Barisani-Asenbauer, Talin",
year = "2016",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization",
number = "12",
volume = "57",
url = "conv_405"
}
Inić-Kanada, A., Stojanović, M., Marinković, E., Stein, E., Lukić, I., Belij, S., Schuerer, N., Montanaro, J., Ghasemian, E., Đokić, R.,& Barisani-Asenbauer, T.. (2016). Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 57(12).
conv_405
Inić-Kanada A, Stojanović M, Marinković E, Stein E, Lukić I, Belij S, Schuerer N, Montanaro J, Ghasemian E, Đokić R, Barisani-Asenbauer T. Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization. in Investigative Ophthalmology & Visual Science. 2016;57(12).
conv_405 .
Inić-Kanada, Aleksandra, Stojanović, Marijana, Marinković, Emilija, Stein, Elisabeth, Lukić, Ivana, Belij, Sandra, Schuerer, Nadine, Montanaro, Jacqueline, Ghasemian, Ehsan, Đokić, Radmila, Barisani-Asenbauer, Talin, "Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization" in Investigative Ophthalmology & Visual Science, 57, no. 12 (2016),
conv_405 .

Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo

Schuerer, Nadine; Stein, Elisabeth; Inić-Kanada, Aleksandra; Belij, Sandra; Stojanović, Marijana; Montanaro, Jacqueline; Ghasemian, Ehsan; Marinković, Emilija; Filipović, Ana; Barisani-Asenbauer, Talin

(Assoc Research Vision Ophthalmology Inc, Rockville, 2016)

TY  - CONF
AU  - Schuerer, Nadine
AU  - Stein, Elisabeth
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Montanaro, Jacqueline
AU  - Ghasemian, Ehsan
AU  - Marinković, Emilija
AU  - Filipović, Ana
AU  - Barisani-Asenbauer, Talin
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/458
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo
IS  - 12
VL  - 57
UR  - conv_404
ER  - 
@conference{
author = "Schuerer, Nadine and Stein, Elisabeth and Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Montanaro, Jacqueline and Ghasemian, Ehsan and Marinković, Emilija and Filipović, Ana and Barisani-Asenbauer, Talin",
year = "2016",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo",
number = "12",
volume = "57",
url = "conv_404"
}
Schuerer, N., Stein, E., Inić-Kanada, A., Belij, S., Stojanović, M., Montanaro, J., Ghasemian, E., Marinković, E., Filipović, A.,& Barisani-Asenbauer, T.. (2016). Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 57(12).
conv_404
Schuerer N, Stein E, Inić-Kanada A, Belij S, Stojanović M, Montanaro J, Ghasemian E, Marinković E, Filipović A, Barisani-Asenbauer T. Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo. in Investigative Ophthalmology & Visual Science. 2016;57(12).
conv_404 .
Schuerer, Nadine, Stein, Elisabeth, Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Montanaro, Jacqueline, Ghasemian, Ehsan, Marinković, Emilija, Filipović, Ana, Barisani-Asenbauer, Talin, "Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo" in Investigative Ophthalmology & Visual Science, 57, no. 12 (2016),
conv_404 .

Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers

Inić-Kanada, Aleksandra; Stojanović, Marijana; Schlacher, Simone; Stein, Elisabeth; Belij-Rammerstorfer, Sandra; Marinković, Emilija; Lukić, Ivana; Montanaro, Jacqueline; Schuerer, Nadine; Bintner, Nora; Kovačević-Jovanović, Vesna; Krnjaja, Ognjen; Mayr, Ulrike Beate; Lubitz, Werner; Barisani-Asenbauer, Talin

(Public Library Science, San Francisco, 2015)

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Schlacher, Simone
AU  - Stein, Elisabeth
AU  - Belij-Rammerstorfer, Sandra
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Montanaro, Jacqueline
AU  - Schuerer, Nadine
AU  - Bintner, Nora
AU  - Kovačević-Jovanović, Vesna
AU  - Krnjaja, Ognjen
AU  - Mayr, Ulrike Beate
AU  - Lubitz, Werner
AU  - Barisani-Asenbauer, Talin
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/434
AB  - Trachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains the world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As the mechanisms engaged in the pathology of the disease are not fully understood, designing a subunit vaccine specific to chlamydial antigens could improve safety for human use. We propose the delivery of chlamydia-specific antigens to the ocular mucosa using particulate carriers, bacterial ghosts (BGs). We therefore characterized humoral and cellular immune responses after conjunctival and subcutaneous immunization with a N-terminal portion (amino acid 1-893) of the chlamydial polymorphic membrane protein C (PmpC) of Ct serovar B, expressed in probiotic Escherichia coli Nissle 1917 bacterial ghosts (EcN BGs) in BALB/cmice. Three immunizations were performed at two-week intervals, and the immune responses were evaluated two weeks after the final immunization in mice. In a guinea pig model of ocular infection animals were immunized in the same manner as the mice, and protection against challenge was assessed two weeks after the last immunization. N-PmpC was successfully expressed within BGs and delivery to the ocularmucosa was well tolerated without signs of inflammation. N-PmpC- specific mucosal IgA levels in tears yielded significantly increased levels in the group immunized via the conjunctiva compared with the subcutaneously immunized mice. Immunization with N-PmpC EcN BGs via both immunization routes prompted the establishment of an N-PmpC-specific IFN gamma immune response. Immunization via the conjunctiva resulted in a decrease in intensity of the transitional inflammatory reaction in conjunctiva of challenged guinea pigs compared with subcutaneously and non-immunized animals. The delivery of the chlamydial subunit vaccine to the ocular mucosa using a particulate carrier, such as BGs, induced both humoral and cellular immune responses. Further investigations are needed to improve the immunization scheme and dosage.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers
IS  - 12
VL  - 10
DO  - 10.1371/journal.pone.0144380
UR  - conv_375
ER  - 
@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Schlacher, Simone and Stein, Elisabeth and Belij-Rammerstorfer, Sandra and Marinković, Emilija and Lukić, Ivana and Montanaro, Jacqueline and Schuerer, Nadine and Bintner, Nora and Kovačević-Jovanović, Vesna and Krnjaja, Ognjen and Mayr, Ulrike Beate and Lubitz, Werner and Barisani-Asenbauer, Talin",
year = "2015",
abstract = "Trachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains the world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As the mechanisms engaged in the pathology of the disease are not fully understood, designing a subunit vaccine specific to chlamydial antigens could improve safety for human use. We propose the delivery of chlamydia-specific antigens to the ocular mucosa using particulate carriers, bacterial ghosts (BGs). We therefore characterized humoral and cellular immune responses after conjunctival and subcutaneous immunization with a N-terminal portion (amino acid 1-893) of the chlamydial polymorphic membrane protein C (PmpC) of Ct serovar B, expressed in probiotic Escherichia coli Nissle 1917 bacterial ghosts (EcN BGs) in BALB/cmice. Three immunizations were performed at two-week intervals, and the immune responses were evaluated two weeks after the final immunization in mice. In a guinea pig model of ocular infection animals were immunized in the same manner as the mice, and protection against challenge was assessed two weeks after the last immunization. N-PmpC was successfully expressed within BGs and delivery to the ocularmucosa was well tolerated without signs of inflammation. N-PmpC- specific mucosal IgA levels in tears yielded significantly increased levels in the group immunized via the conjunctiva compared with the subcutaneously immunized mice. Immunization with N-PmpC EcN BGs via both immunization routes prompted the establishment of an N-PmpC-specific IFN gamma immune response. Immunization via the conjunctiva resulted in a decrease in intensity of the transitional inflammatory reaction in conjunctiva of challenged guinea pigs compared with subcutaneously and non-immunized animals. The delivery of the chlamydial subunit vaccine to the ocular mucosa using a particulate carrier, such as BGs, induced both humoral and cellular immune responses. Further investigations are needed to improve the immunization scheme and dosage.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers",
number = "12",
volume = "10",
doi = "10.1371/journal.pone.0144380",
url = "conv_375"
}
Inić-Kanada, A., Stojanović, M., Schlacher, S., Stein, E., Belij-Rammerstorfer, S., Marinković, E., Lukić, I., Montanaro, J., Schuerer, N., Bintner, N., Kovačević-Jovanović, V., Krnjaja, O., Mayr, U. B., Lubitz, W.,& Barisani-Asenbauer, T.. (2015). Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers. in PLoS One
Public Library Science, San Francisco., 10(12).
https://doi.org/10.1371/journal.pone.0144380
conv_375
Inić-Kanada A, Stojanović M, Schlacher S, Stein E, Belij-Rammerstorfer S, Marinković E, Lukić I, Montanaro J, Schuerer N, Bintner N, Kovačević-Jovanović V, Krnjaja O, Mayr UB, Lubitz W, Barisani-Asenbauer T. Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers. in PLoS One. 2015;10(12).
doi:10.1371/journal.pone.0144380
conv_375 .
Inić-Kanada, Aleksandra, Stojanović, Marijana, Schlacher, Simone, Stein, Elisabeth, Belij-Rammerstorfer, Sandra, Marinković, Emilija, Lukić, Ivana, Montanaro, Jacqueline, Schuerer, Nadine, Bintner, Nora, Kovačević-Jovanović, Vesna, Krnjaja, Ognjen, Mayr, Ulrike Beate, Lubitz, Werner, Barisani-Asenbauer, Talin, "Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers" in PLoS One, 10, no. 12 (2015),
https://doi.org/10.1371/journal.pone.0144380 .,
conv_375 .
7
18
14
16

Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis

Inić-Kanada, Aleksandra; Belij, Sandra; Stojanović, Marijana; Marinković, Emilija; Stojičević, Ivana; Montanaro, Jacqueline; Stein, Elisabeth; Bintner, Nora; Schuerer, Nadine; Ladurner, Angela; Barisani-Asenbauer, Talin

(Wiley-Blackwell, Hoboken, 2014)

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Montanaro, Jacqueline
AU  - Stein, Elisabeth
AU  - Bintner, Nora
AU  - Schuerer, Nadine
AU  - Ladurner, Angela
AU  - Barisani-Asenbauer, Talin
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/404
PB  - Wiley-Blackwell, Hoboken
C3  - Immunology
T1  - Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis
EP  - 25
SP  - 25
VL  - 143
UR  - conv_344
ER  - 
@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Montanaro, Jacqueline and Stein, Elisabeth and Bintner, Nora and Schuerer, Nadine and Ladurner, Angela and Barisani-Asenbauer, Talin",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Immunology",
title = "Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis",
pages = "25-25",
volume = "143",
url = "conv_344"
}
Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Montanaro, J., Stein, E., Bintner, N., Schuerer, N., Ladurner, A.,& Barisani-Asenbauer, T.. (2014). Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis. in Immunology
Wiley-Blackwell, Hoboken., 143, 25-25.
conv_344
Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Montanaro J, Stein E, Bintner N, Schuerer N, Ladurner A, Barisani-Asenbauer T. Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis. in Immunology. 2014;143:25-25.
conv_344 .
Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Montanaro, Jacqueline, Stein, Elisabeth, Bintner, Nora, Schuerer, Nadine, Ladurner, Angela, Barisani-Asenbauer, Talin, "Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis" in Immunology, 143 (2014):25-25,
conv_344 .