Kovačević-Jovanović, Vesna

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  • Kovačević-Jovanović, Vesna (37)
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Author's Bibliography

Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?

Blagojević, Veljko; Kovačević-Jovanović, Vesna; Ćuruvija, Ivana; Petrović, Raisa; Vujnović, Ivana; Vujić, Vesna; Stanojević, Stanislava

(Pergamon-Elsevier Science Ltd, Oxford, 2018)

TY  - JOUR
AU  - Blagojević, Veljko
AU  - Kovačević-Jovanović, Vesna
AU  - Ćuruvija, Ivana
AU  - Petrović, Raisa
AU  - Vujnović, Ivana
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/512
AB  - Aims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?
EP  - 157
SP  - 147
VL  - 197
DO  - 10.1016/j.lfs.2018.02.011
UR  - conv_426
ER  - 
@article{
author = "Blagojević, Veljko and Kovačević-Jovanović, Vesna and Ćuruvija, Ivana and Petrović, Raisa and Vujnović, Ivana and Vujić, Vesna and Stanojević, Stanislava",
year = "2018",
abstract = "Aims: Some gut commensals can be protective, whereas others are implicated as necessary for development of inflammatory/autoimmune diseases. Peritoneal immune cells may play an important role in promoting auto-immunity in response to gut microbiota. This study investigated the phenotype and the function of peritoneal immune cells in the autoimmunity-resistant Albino Oxford (AO), and the autoimmunity-prone Dark Agouti (DA) rat strains upon stimulation with their own colonic E. coli or Enterococcus. Main methods: Rats were intraperitoneally injected with their own E. coli or Enterococcus. Peritoneal cells isolated two days later were tested for nitric oxide (NO) and cytokine production, and for arginase and myeloperoxidase (MPO) activity. The phenotype of cells was determined using flow cytometry. Key findings: While the Enterococcus injection did not affect the composition of peritoneal cells in AO rats, the E. coli treatment increased the percentages of activated CD11b(int)HIS48(hi) neutrophils, and decreased the proportion of resident (CD11b(hi)HIS48(int/low), CD163+ CD86+) and anti-inflammatory CD68+ CD206+ macrophages. E. coli increased the production of NO and urea, but preserved their ratio in cells from AO rats. Conversely, both E. coli and Enterococcus diminished the proportion of resident and anti-inflammatory macrophages, increased the proportion of activated neutrophils, and induced inflammatory polarization of peritoneal cells in DA rats. However, injection of E. coli maintained the ratio of typical CD11b(int)HIS48(int) neutrophils in DA rats, which correlated with the sustained MPO activity. Significance: The rat strain differences in peritoneal cell response to own commensal microbiota may contribute to differential susceptibility to inflammatory/autoimmune diseases.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?",
pages = "157-147",
volume = "197",
doi = "10.1016/j.lfs.2018.02.011",
url = "conv_426"
}
Blagojević, V., Kovačević-Jovanović, V., Ćuruvija, I., Petrović, R., Vujnović, I., Vujić, V.,& Stanojević, S.. (2018). Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 197, 147-157.
https://doi.org/10.1016/j.lfs.2018.02.011
conv_426
Blagojević V, Kovačević-Jovanović V, Ćuruvija I, Petrović R, Vujnović I, Vujić V, Stanojević S. Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?. in Life Sciences. 2018;197:147-157.
doi:10.1016/j.lfs.2018.02.011
conv_426 .
Blagojević, Veljko, Kovačević-Jovanović, Vesna, Ćuruvija, Ivana, Petrović, Raisa, Vujnović, Ivana, Vujić, Vesna, Stanojević, Stanislava, "Rat strain differences in peritoneal immune cell response to selected gut microbiota: A crossroad between tolerance and autoimmunity?" in Life Sciences, 197 (2018):147-157,
https://doi.org/10.1016/j.lfs.2018.02.011 .,
conv_426 .
1
4
3
4

Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis

Stanojević, Stanislava; Blagojević, Veljko; Ćuruvija, Ivana; Veljović, Katarina; Soković-Bajić, Svetlana; Kotur-Stevuljević, Jelena; Bogdanović, Andrija; Petrović, Raisa; Vujnović, Ivana; Kovačević-Jovanović, Vesna

(Elsevier Science Bv, Amsterdam, 2018)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Veljović, Katarina
AU  - Soković-Bajić, Svetlana
AU  - Kotur-Stevuljević, Jelena
AU  - Bogdanović, Andrija
AU  - Petrović, Raisa
AU  - Vujnović, Ivana
AU  - Kovačević-Jovanović, Vesna
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/505
AB  - The current study investigated a potential modulating effect of orally applied Lactobacillus rhamnosus 64 (LB64) during the early postnatal period (day of life: similar to 3-30), during young adult period (day of life: 31-70) or throughout experiment, on parameters of trinitrobenzenesulfonic acid (TNBS)-induced colitis in adult rats. Treatment with LB64 during early postnatal, but not during young adult period reduced clinical damage score, neutrophil and macrophage infiltration into colon, the level of cytokine and myeloperoxidase (MPO) activity, but had no influence on other parameters of oxidative damage. Early postnatal treatment with LB64 also increased the diversity of fecal Bifidobacteria and Eubacteria, and improved maturation of ileal villi in 30-days old rats. When LB64 is applied during a critical period early in life, it affects immune system functioning of adults, probably by interactions with the mucosal immune system of the gastrointestinal tract that provides immune system maturation and shapes the overall immune response.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis
EP  - 105
SP  - 92
VL  - 48
DO  - 10.1016/j.jff.2018.07.014
UR  - conv_442
ER  - 
@article{
author = "Stanojević, Stanislava and Blagojević, Veljko and Ćuruvija, Ivana and Veljović, Katarina and Soković-Bajić, Svetlana and Kotur-Stevuljević, Jelena and Bogdanović, Andrija and Petrović, Raisa and Vujnović, Ivana and Kovačević-Jovanović, Vesna",
year = "2018",
abstract = "The current study investigated a potential modulating effect of orally applied Lactobacillus rhamnosus 64 (LB64) during the early postnatal period (day of life: similar to 3-30), during young adult period (day of life: 31-70) or throughout experiment, on parameters of trinitrobenzenesulfonic acid (TNBS)-induced colitis in adult rats. Treatment with LB64 during early postnatal, but not during young adult period reduced clinical damage score, neutrophil and macrophage infiltration into colon, the level of cytokine and myeloperoxidase (MPO) activity, but had no influence on other parameters of oxidative damage. Early postnatal treatment with LB64 also increased the diversity of fecal Bifidobacteria and Eubacteria, and improved maturation of ileal villi in 30-days old rats. When LB64 is applied during a critical period early in life, it affects immune system functioning of adults, probably by interactions with the mucosal immune system of the gastrointestinal tract that provides immune system maturation and shapes the overall immune response.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis",
pages = "105-92",
volume = "48",
doi = "10.1016/j.jff.2018.07.014",
url = "conv_442"
}
Stanojević, S., Blagojević, V., Ćuruvija, I., Veljović, K., Soković-Bajić, S., Kotur-Stevuljević, J., Bogdanović, A., Petrović, R., Vujnović, I.,& Kovačević-Jovanović, V.. (2018). Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 48, 92-105.
https://doi.org/10.1016/j.jff.2018.07.014
conv_442
Stanojević S, Blagojević V, Ćuruvija I, Veljović K, Soković-Bajić S, Kotur-Stevuljević J, Bogdanović A, Petrović R, Vujnović I, Kovačević-Jovanović V. Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis. in Journal of Functional Foods. 2018;48:92-105.
doi:10.1016/j.jff.2018.07.014
conv_442 .
Stanojević, Stanislava, Blagojević, Veljko, Ćuruvija, Ivana, Veljović, Katarina, Soković-Bajić, Svetlana, Kotur-Stevuljević, Jelena, Bogdanović, Andrija, Petrović, Raisa, Vujnović, Ivana, Kovačević-Jovanović, Vesna, "Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis" in Journal of Functional Foods, 48 (2018):92-105,
https://doi.org/10.1016/j.jff.2018.07.014 .,
conv_442 .
6
2
2
2

Resurgence of measles in Serbia 2010-2011 highlights the need for supplementary immunization activities

Nedeljković, Jasminka; Rakić-Adrović, Slavica; Tasić, G.; Kovačević-Jovanović, Vesna; Lončarević, Goranka; Huebschen, Judith M.; Muller, Claude P.

(Cambridge Univ Press, New York, 2016)

TY  - JOUR
AU  - Nedeljković, Jasminka
AU  - Rakić-Adrović, Slavica
AU  - Tasić, G.
AU  - Kovačević-Jovanović, Vesna
AU  - Lončarević, Goranka
AU  - Huebschen, Judith M.
AU  - Muller, Claude P.
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/468
AB  - Between December 2010 and August 2011 an outbreak of measles occurred in Serbia with 363 reported cases. Sera and/or nose/throat swabs were collected from 193 patients and tested for measles-specific IgM antibodies by ELISA and viral RNA by RT-PCR, respectively. Epidemiological data were obtained from the surveillance database of the Institute of Public Health of Serbia. Of the 363 cases involved in the outbreak, 113 were laboratory confirmed. More than one third of the patients were hospitalized (n = 130, 35.8%) and for 15 (4.1% of the reported outbreak cases) the infection was complicated by pneumonia. Mostly pre-school children aged  lt = 4 years (37.8%) and adults aged  gt = 30 years (27.3%) were affected. The majority of patients belonged to the Roma population with a preponderance of female cases (57.0%). Nearly 94% of the patients were either unvaccinated or of unknown vaccination status. The main outbreak virus was the D4-Hamburg strain. The outbreak in Serbia occurred after several years of very low measles incidence despite a high routine immunization coverage in the general population, suggesting that special efforts to identify and vaccinate susceptible population groups are required even in countries with apparently good disease control.
PB  - Cambridge Univ Press, New York
T2  - Epidemiology and Infection
T1  - Resurgence of measles in Serbia 2010-2011 highlights the need for supplementary immunization activities
EP  - 1128
IS  - 5
SP  - 1121
VL  - 144
DO  - 10.1017/S0950268815002277
UR  - conv_377
ER  - 
@article{
author = "Nedeljković, Jasminka and Rakić-Adrović, Slavica and Tasić, G. and Kovačević-Jovanović, Vesna and Lončarević, Goranka and Huebschen, Judith M. and Muller, Claude P.",
year = "2016",
abstract = "Between December 2010 and August 2011 an outbreak of measles occurred in Serbia with 363 reported cases. Sera and/or nose/throat swabs were collected from 193 patients and tested for measles-specific IgM antibodies by ELISA and viral RNA by RT-PCR, respectively. Epidemiological data were obtained from the surveillance database of the Institute of Public Health of Serbia. Of the 363 cases involved in the outbreak, 113 were laboratory confirmed. More than one third of the patients were hospitalized (n = 130, 35.8%) and for 15 (4.1% of the reported outbreak cases) the infection was complicated by pneumonia. Mostly pre-school children aged  lt = 4 years (37.8%) and adults aged  gt = 30 years (27.3%) were affected. The majority of patients belonged to the Roma population with a preponderance of female cases (57.0%). Nearly 94% of the patients were either unvaccinated or of unknown vaccination status. The main outbreak virus was the D4-Hamburg strain. The outbreak in Serbia occurred after several years of very low measles incidence despite a high routine immunization coverage in the general population, suggesting that special efforts to identify and vaccinate susceptible population groups are required even in countries with apparently good disease control.",
publisher = "Cambridge Univ Press, New York",
journal = "Epidemiology and Infection",
title = "Resurgence of measles in Serbia 2010-2011 highlights the need for supplementary immunization activities",
pages = "1128-1121",
number = "5",
volume = "144",
doi = "10.1017/S0950268815002277",
url = "conv_377"
}
Nedeljković, J., Rakić-Adrović, S., Tasić, G., Kovačević-Jovanović, V., Lončarević, G., Huebschen, J. M.,& Muller, C. P.. (2016). Resurgence of measles in Serbia 2010-2011 highlights the need for supplementary immunization activities. in Epidemiology and Infection
Cambridge Univ Press, New York., 144(5), 1121-1128.
https://doi.org/10.1017/S0950268815002277
conv_377
Nedeljković J, Rakić-Adrović S, Tasić G, Kovačević-Jovanović V, Lončarević G, Huebschen JM, Muller CP. Resurgence of measles in Serbia 2010-2011 highlights the need for supplementary immunization activities. in Epidemiology and Infection. 2016;144(5):1121-1128.
doi:10.1017/S0950268815002277
conv_377 .
Nedeljković, Jasminka, Rakić-Adrović, Slavica, Tasić, G., Kovačević-Jovanović, Vesna, Lončarević, Goranka, Huebschen, Judith M., Muller, Claude P., "Resurgence of measles in Serbia 2010-2011 highlights the need for supplementary immunization activities" in Epidemiology and Infection, 144, no. 5 (2016):1121-1128,
https://doi.org/10.1017/S0950268815002277 .,
conv_377 .
10
9
10

Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat

Stanojević, Stanislava; Kovačević-Jovanović, Vesna; Dimitrijević, Mirjana; Vujić, Vesna; Ćuruvija, Ivana; Blagojević, Veljko; Leposavić, Gordana

(Wiley, Hoboken, 2015)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Ćuruvija, Ivana
AU  - Blagojević, Veljko
AU  - Leposavić, Gordana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/423
AB  - Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance.
PB  - Wiley, Hoboken
T2  - American Journal of Reproductive Immunology
T1  - Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat
EP  - 456
IS  - 5
SP  - 445
VL  - 74
DO  - 10.1111/aji.12424
UR  - conv_373
ER  - 
@article{
author = "Stanojević, Stanislava and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana and Vujić, Vesna and Ćuruvija, Ivana and Blagojević, Veljko and Leposavić, Gordana",
year = "2015",
abstract = "Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance.",
publisher = "Wiley, Hoboken",
journal = "American Journal of Reproductive Immunology",
title = "Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat",
pages = "456-445",
number = "5",
volume = "74",
doi = "10.1111/aji.12424",
url = "conv_373"
}
Stanojević, S., Kovačević-Jovanović, V., Dimitrijević, M., Vujić, V., Ćuruvija, I., Blagojević, V.,& Leposavić, G.. (2015). Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat. in American Journal of Reproductive Immunology
Wiley, Hoboken., 74(5), 445-456.
https://doi.org/10.1111/aji.12424
conv_373
Stanojević S, Kovačević-Jovanović V, Dimitrijević M, Vujić V, Ćuruvija I, Blagojević V, Leposavić G. Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat. in American Journal of Reproductive Immunology. 2015;74(5):445-456.
doi:10.1111/aji.12424
conv_373 .
Stanojević, Stanislava, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, Vujić, Vesna, Ćuruvija, Ivana, Blagojević, Veljko, Leposavić, Gordana, "Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat" in American Journal of Reproductive Immunology, 74, no. 5 (2015):445-456,
https://doi.org/10.1111/aji.12424 .,
conv_373 .
1
3
2
3

Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats

Kovačević-Jovanović, Vesna; Miletić, Tatjana; Stanojević, Stanislava; Mitić, Katarina; Dimitrijević, Mirjana

(Akademiai Kiado Zrt, Budapest, 2015)

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/449
AB  - We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats
EP  - 19
IS  - 1
SP  - 1
VL  - 62
DO  - 10.1556/AMicr.62.2015.1.1
UR  - conv_359
ER  - 
@article{
author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2015",
abstract = "We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specific to proteins of Escherichia coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to Escherichia coli, especially of the IgG2b antibody class. Intramuscular administration of Escherichia coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3(+)CD26(+) cell percentage were found in AO rats. The observed correlation between the serologic response to commensal flora and rats' genetic background as a defining factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats",
pages = "19-1",
number = "1",
volume = "62",
doi = "10.1556/AMicr.62.2015.1.1",
url = "conv_359"
}
Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2015). Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Zrt, Budapest., 62(1), 1-19.
https://doi.org/10.1556/AMicr.62.2015.1.1
conv_359
Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats. in Acta Microbiologica et Immunologica Hungarica. 2015;62(1):1-19.
doi:10.1556/AMicr.62.2015.1.1
conv_359 .
Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Immune response to gut escherichia coli and susceptibility to adjuvant arthritis in the rats" in Acta Microbiologica et Immunologica Hungarica, 62, no. 1 (2015):1-19,
https://doi.org/10.1556/AMicr.62.2015.1.1 .,
conv_359 .
1
6
6
6

Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers

Inić-Kanada, Aleksandra; Stojanović, Marijana; Schlacher, Simone; Stein, Elisabeth; Belij-Rammerstorfer, Sandra; Marinković, Emilija; Lukić, Ivana; Montanaro, Jacqueline; Schuerer, Nadine; Bintner, Nora; Kovačević-Jovanović, Vesna; Krnjaja, Ognjen; Mayr, Ulrike Beate; Lubitz, Werner; Barisani-Asenbauer, Talin

(Public Library Science, San Francisco, 2015)

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Schlacher, Simone
AU  - Stein, Elisabeth
AU  - Belij-Rammerstorfer, Sandra
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Montanaro, Jacqueline
AU  - Schuerer, Nadine
AU  - Bintner, Nora
AU  - Kovačević-Jovanović, Vesna
AU  - Krnjaja, Ognjen
AU  - Mayr, Ulrike Beate
AU  - Lubitz, Werner
AU  - Barisani-Asenbauer, Talin
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/434
AB  - Trachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains the world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As the mechanisms engaged in the pathology of the disease are not fully understood, designing a subunit vaccine specific to chlamydial antigens could improve safety for human use. We propose the delivery of chlamydia-specific antigens to the ocular mucosa using particulate carriers, bacterial ghosts (BGs). We therefore characterized humoral and cellular immune responses after conjunctival and subcutaneous immunization with a N-terminal portion (amino acid 1-893) of the chlamydial polymorphic membrane protein C (PmpC) of Ct serovar B, expressed in probiotic Escherichia coli Nissle 1917 bacterial ghosts (EcN BGs) in BALB/cmice. Three immunizations were performed at two-week intervals, and the immune responses were evaluated two weeks after the final immunization in mice. In a guinea pig model of ocular infection animals were immunized in the same manner as the mice, and protection against challenge was assessed two weeks after the last immunization. N-PmpC was successfully expressed within BGs and delivery to the ocularmucosa was well tolerated without signs of inflammation. N-PmpC- specific mucosal IgA levels in tears yielded significantly increased levels in the group immunized via the conjunctiva compared with the subcutaneously immunized mice. Immunization with N-PmpC EcN BGs via both immunization routes prompted the establishment of an N-PmpC-specific IFN gamma immune response. Immunization via the conjunctiva resulted in a decrease in intensity of the transitional inflammatory reaction in conjunctiva of challenged guinea pigs compared with subcutaneously and non-immunized animals. The delivery of the chlamydial subunit vaccine to the ocular mucosa using a particulate carrier, such as BGs, induced both humoral and cellular immune responses. Further investigations are needed to improve the immunization scheme and dosage.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers
IS  - 12
VL  - 10
DO  - 10.1371/journal.pone.0144380
UR  - conv_375
ER  - 
@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Schlacher, Simone and Stein, Elisabeth and Belij-Rammerstorfer, Sandra and Marinković, Emilija and Lukić, Ivana and Montanaro, Jacqueline and Schuerer, Nadine and Bintner, Nora and Kovačević-Jovanović, Vesna and Krnjaja, Ognjen and Mayr, Ulrike Beate and Lubitz, Werner and Barisani-Asenbauer, Talin",
year = "2015",
abstract = "Trachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains the world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As the mechanisms engaged in the pathology of the disease are not fully understood, designing a subunit vaccine specific to chlamydial antigens could improve safety for human use. We propose the delivery of chlamydia-specific antigens to the ocular mucosa using particulate carriers, bacterial ghosts (BGs). We therefore characterized humoral and cellular immune responses after conjunctival and subcutaneous immunization with a N-terminal portion (amino acid 1-893) of the chlamydial polymorphic membrane protein C (PmpC) of Ct serovar B, expressed in probiotic Escherichia coli Nissle 1917 bacterial ghosts (EcN BGs) in BALB/cmice. Three immunizations were performed at two-week intervals, and the immune responses were evaluated two weeks after the final immunization in mice. In a guinea pig model of ocular infection animals were immunized in the same manner as the mice, and protection against challenge was assessed two weeks after the last immunization. N-PmpC was successfully expressed within BGs and delivery to the ocularmucosa was well tolerated without signs of inflammation. N-PmpC- specific mucosal IgA levels in tears yielded significantly increased levels in the group immunized via the conjunctiva compared with the subcutaneously immunized mice. Immunization with N-PmpC EcN BGs via both immunization routes prompted the establishment of an N-PmpC-specific IFN gamma immune response. Immunization via the conjunctiva resulted in a decrease in intensity of the transitional inflammatory reaction in conjunctiva of challenged guinea pigs compared with subcutaneously and non-immunized animals. The delivery of the chlamydial subunit vaccine to the ocular mucosa using a particulate carrier, such as BGs, induced both humoral and cellular immune responses. Further investigations are needed to improve the immunization scheme and dosage.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers",
number = "12",
volume = "10",
doi = "10.1371/journal.pone.0144380",
url = "conv_375"
}
Inić-Kanada, A., Stojanović, M., Schlacher, S., Stein, E., Belij-Rammerstorfer, S., Marinković, E., Lukić, I., Montanaro, J., Schuerer, N., Bintner, N., Kovačević-Jovanović, V., Krnjaja, O., Mayr, U. B., Lubitz, W.,& Barisani-Asenbauer, T.. (2015). Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers. in PLoS One
Public Library Science, San Francisco., 10(12).
https://doi.org/10.1371/journal.pone.0144380
conv_375
Inić-Kanada A, Stojanović M, Schlacher S, Stein E, Belij-Rammerstorfer S, Marinković E, Lukić I, Montanaro J, Schuerer N, Bintner N, Kovačević-Jovanović V, Krnjaja O, Mayr UB, Lubitz W, Barisani-Asenbauer T. Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers. in PLoS One. 2015;10(12).
doi:10.1371/journal.pone.0144380
conv_375 .
Inić-Kanada, Aleksandra, Stojanović, Marijana, Schlacher, Simone, Stein, Elisabeth, Belij-Rammerstorfer, Sandra, Marinković, Emilija, Lukić, Ivana, Montanaro, Jacqueline, Schuerer, Nadine, Bintner, Nora, Kovačević-Jovanović, Vesna, Krnjaja, Ognjen, Mayr, Ulrike Beate, Lubitz, Werner, Barisani-Asenbauer, Talin, "Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers" in PLoS One, 10, no. 12 (2015),
https://doi.org/10.1371/journal.pone.0144380 .,
conv_375 .
7
18
14
16

A Mumps Outbreak in Vojvodina, Serbia, in 2012 Underlines the Need for Additional Vaccination Opportunities for Young Adults

Nedeljković, Jasminka; Kovačević-Jovanović, Vesna; Milošević, Vesna; Šeguljev, Zorica; Petrović, Vladimir; Muller, Claude P.; Huebschen, Judith M.

(Public Library Science, San Francisco, 2015)

TY  - JOUR
AU  - Nedeljković, Jasminka
AU  - Kovačević-Jovanović, Vesna
AU  - Milošević, Vesna
AU  - Šeguljev, Zorica
AU  - Petrović, Vladimir
AU  - Muller, Claude P.
AU  - Huebschen, Judith M.
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/438
AB  - In 2012, mumps was introduced from Bosnia and Herzegovina to Vojvodina, causing an outbreak with 335 reported cases. The present manuscript analyses the epidemiological and laboratory characteristics of this outbreak, identifies its main causes and suggests potential future preventive measures. Sera of 133 patients were tested for mumps-specific antibodies by ELISA and 15 nose/throat swabs were investigated for mumps virus RNA by RT-PCR. IgG antibodies were found in 127 patients (95.5%). Mumps infection was laboratory-confirmed in 53 patients, including 44 IgM and 9 PCR positive cases. All other 282 cases were classified as epidemiologically-confirmed. More than half of the patients (n = 181, 54%) were 20-29 years old, followed by the 15-19 age bracket (n = 95, 28.4%). Twice as many males as females were affected (67% versus 33%). Disease complications were reported in 13 cases (3.9%), including 9 patients with orchitis and 4 with pancreatitis. According to medical records or anamnestic data, 190 patients (56.7%) were immunized with two doses and 35 (10.4%) with one dose of mumps-containing vaccine. The Serbian sequences corresponded to a minor genotype G variant detected during the 2011/2012 mumps outbreak in Bosnia and Herzegovina. Vaccine failures, the initial one-dose immunization policy and a vaccine shortage between 1999 and 2002 contributed to the outbreak. Additional vaccination opportunities should be offered to young adults during transition periods in their life trajectories.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - A Mumps Outbreak in Vojvodina, Serbia, in 2012 Underlines the Need for Additional Vaccination Opportunities for Young Adults
IS  - 10
VL  - 10
DO  - 10.1371/journal.pone.0139815
UR  - conv_371
ER  - 
@article{
author = "Nedeljković, Jasminka and Kovačević-Jovanović, Vesna and Milošević, Vesna and Šeguljev, Zorica and Petrović, Vladimir and Muller, Claude P. and Huebschen, Judith M.",
year = "2015",
abstract = "In 2012, mumps was introduced from Bosnia and Herzegovina to Vojvodina, causing an outbreak with 335 reported cases. The present manuscript analyses the epidemiological and laboratory characteristics of this outbreak, identifies its main causes and suggests potential future preventive measures. Sera of 133 patients were tested for mumps-specific antibodies by ELISA and 15 nose/throat swabs were investigated for mumps virus RNA by RT-PCR. IgG antibodies were found in 127 patients (95.5%). Mumps infection was laboratory-confirmed in 53 patients, including 44 IgM and 9 PCR positive cases. All other 282 cases were classified as epidemiologically-confirmed. More than half of the patients (n = 181, 54%) were 20-29 years old, followed by the 15-19 age bracket (n = 95, 28.4%). Twice as many males as females were affected (67% versus 33%). Disease complications were reported in 13 cases (3.9%), including 9 patients with orchitis and 4 with pancreatitis. According to medical records or anamnestic data, 190 patients (56.7%) were immunized with two doses and 35 (10.4%) with one dose of mumps-containing vaccine. The Serbian sequences corresponded to a minor genotype G variant detected during the 2011/2012 mumps outbreak in Bosnia and Herzegovina. Vaccine failures, the initial one-dose immunization policy and a vaccine shortage between 1999 and 2002 contributed to the outbreak. Additional vaccination opportunities should be offered to young adults during transition periods in their life trajectories.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "A Mumps Outbreak in Vojvodina, Serbia, in 2012 Underlines the Need for Additional Vaccination Opportunities for Young Adults",
number = "10",
volume = "10",
doi = "10.1371/journal.pone.0139815",
url = "conv_371"
}
Nedeljković, J., Kovačević-Jovanović, V., Milošević, V., Šeguljev, Z., Petrović, V., Muller, C. P.,& Huebschen, J. M.. (2015). A Mumps Outbreak in Vojvodina, Serbia, in 2012 Underlines the Need for Additional Vaccination Opportunities for Young Adults. in PLoS One
Public Library Science, San Francisco., 10(10).
https://doi.org/10.1371/journal.pone.0139815
conv_371
Nedeljković J, Kovačević-Jovanović V, Milošević V, Šeguljev Z, Petrović V, Muller CP, Huebschen JM. A Mumps Outbreak in Vojvodina, Serbia, in 2012 Underlines the Need for Additional Vaccination Opportunities for Young Adults. in PLoS One. 2015;10(10).
doi:10.1371/journal.pone.0139815
conv_371 .
Nedeljković, Jasminka, Kovačević-Jovanović, Vesna, Milošević, Vesna, Šeguljev, Zorica, Petrović, Vladimir, Muller, Claude P., Huebschen, Judith M., "A Mumps Outbreak in Vojvodina, Serbia, in 2012 Underlines the Need for Additional Vaccination Opportunities for Young Adults" in PLoS One, 10, no. 10 (2015),
https://doi.org/10.1371/journal.pone.0139815 .,
conv_371 .
1
8
8
7

Strain differences in the humoral immune response to commensal bacterial antigens in rats

Kovačević-Jovanović, Vesna; Miletić, Tatjana; Stanojević, Stanislava; Mitić, Katarina; Dimitrijević, Mirjana

(Akademiai Kiado Rt, Budapest, 2013)

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/386
AB  - We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Strain differences in the humoral immune response to commensal bacterial antigens in rats
EP  - 288
IS  - 3
SP  - 271
VL  - 60
DO  - 10.1556/AMicr.60.2013.3.4
UR  - conv_318
ER  - 
@article{
author = "Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2013",
abstract = "We have investigated the immune response to commensal bacterial species in the two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). The predominant Gram-negative aerobe in our rats' intestinal bacterial flora was Escherichia coli, while Proteus mirabilis was isolated only from DA rat strain. We report that sera from both DA and AO rat strains contain specific IgG against predominant intestinal flora. Intramuscular administration of commensal bacterial antigens provoked only Th1-type antibody response in AO rats while DA rats developed mixed Th1- and Th2-type antibody response to E. coli and Th1-type response to P. mirabilis antigens. Weaker antibody production to own E. coli and higher serum levels of natural IgG and IgA P. mirabilis-specific antibodies combined with higher CD3+ cells proliferation was found in AO rats. Strain difference in the pattern of antibody production and differential regulation of immune response to commensal bacteria may contribute to the marked differences in the immune reactivity of AO and DA rats.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Strain differences in the humoral immune response to commensal bacterial antigens in rats",
pages = "288-271",
number = "3",
volume = "60",
doi = "10.1556/AMicr.60.2013.3.4",
url = "conv_318"
}
Kovačević-Jovanović, V., Miletić, T., Stanojević, S., Mitić, K.,& Dimitrijević, M.. (2013). Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Rt, Budapest., 60(3), 271-288.
https://doi.org/10.1556/AMicr.60.2013.3.4
conv_318
Kovačević-Jovanović V, Miletić T, Stanojević S, Mitić K, Dimitrijević M. Strain differences in the humoral immune response to commensal bacterial antigens in rats. in Acta Microbiologica et Immunologica Hungarica. 2013;60(3):271-288.
doi:10.1556/AMicr.60.2013.3.4
conv_318 .
Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, Mitić, Katarina, Dimitrijević, Mirjana, "Strain differences in the humoral immune response to commensal bacterial antigens in rats" in Acta Microbiologica et Immunologica Hungarica, 60, no. 3 (2013):271-288,
https://doi.org/10.1556/AMicr.60.2013.3.4 .,
conv_318 .
3
3
4

Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors

Kuštrimović, Nataša; Mitić, Katarina; Dimitrijević, Mirjana; Vujić, Vesna; Kovačević-Jovanović, Vesna; Miletić, Tatjana; Stanojević, Stanislava

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2011)

TY  - JOUR
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Stanojević, Stanislava
PY  - 2011
UR  - http://intor.torlakinstitut.com/handle/123456789/326
AB  - The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection.
AB  - Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors
T1  - Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora
EP  - 132
IS  - 2-3
SP  - 119
VL  - 61
DO  - 10.2298/AVB1103119K
UR  - conv_58
ER  - 
@article{
author = "Kuštrimović, Nataša and Mitić, Katarina and Dimitrijević, Mirjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Stanojević, Stanislava",
year = "2011",
abstract = "The present study tests the hypothesis that the difference in the intensity of paw edema found between the Dark Agouti (DA) and Albino Oxford (AO) rat strains originates from the distinct participation of histamine, serotonin and their corresponding receptors in Concanavalin A (Con A)-induced inflammation. DA and AO male rats were intraplantarly injected with specific receptor antagonists prior to Con A, and the intensity of inflammation was determined by measuring the paw diameter. Our results have showed that histamine H1 and H2 receptor antagonists reduced the Con A-induced paw edema in DA rats, while serotonin 5HT3 receptor antagonist diminished the inflammation in both DA and AO rat strains. The calcium channel blocker did not change Con A-induced inflammation. Strain differences in the intensity and kinetics of inflammation observed between the DA and AO rats are most likely defined by the diversity of mediators released and their receptors activated upon Con A injection., Testirana je hipoteza da razlike u intenzitetu inflamatornog edema šape indukovanog konkanavalinom A u pacova Dark Agouti (DA) i Albino Oxford (AO) soja potiču od različitog doprinosa histamina i serotonina i njihovih odgovarajućih receptora. Mužjaci pacova DA i AO soja su intraplantarno tretirani antagonistima specifičnih receptora pre izazivanja inflamacije konkanavalinom A i intenzitet inflamacije je praćen merenjem dijametra šape. Naši rezultati su ukazali da antagonisti histaminskih H1 i H2 receptora smanjuju edem šape indukovan konkanavalinom A u DA pacova, dok antagonist serotoninskih 5HT3 receptora smanjuje edem šape u oba soja pacova. Blokator kalcijumskih kanala ne utiče na inflamaciju izazvanu konkanavalinom A. Razlike u intenzitetu i kinetici inflamatornog odgovora indukovanog konkanavalinom A između DA i AO sojeva su najverovatnije posledica razlika u oslobođ enim medijatorima i aktivaciji odgovarajućih receptora nakon injekcije konkanavalina A.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors, Razlike u edemu šape pacova indukovanom konkanavalinom a u zavisnosti od soja - uticaj histaminskih H1 i H2 receptora",
pages = "132-119",
number = "2-3",
volume = "61",
doi = "10.2298/AVB1103119K",
url = "conv_58"
}
Kuštrimović, N., Mitić, K., Dimitrijević, M., Vujić, V., Kovačević-Jovanović, V., Miletić, T.,& Stanojević, S.. (2011). Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 119-132.
https://doi.org/10.2298/AVB1103119K
conv_58
Kuštrimović N, Mitić K, Dimitrijević M, Vujić V, Kovačević-Jovanović V, Miletić T, Stanojević S. Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors. in Acta veterinaria - Beograd. 2011;61(2-3):119-132.
doi:10.2298/AVB1103119K
conv_58 .
Kuštrimović, Nataša, Mitić, Katarina, Dimitrijević, Mirjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Stanojević, Stanislava, "Strain differences in concanavalin a-induced paw edema in the rat: Involvement of histamine H1 and H2 receptors" in Acta veterinaria - Beograd, 61, no. 2-3 (2011):119-132,
https://doi.org/10.2298/AVB1103119K .,
conv_58 .
1
1
1

Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity

Dimitrijević, Mirjana; Stanojević, Stanislava; Mitić, Katarina; Kuštrimović, Nataša; Vujić, Vesna; Miletić, Tatjana; Kovačević-Jovanović, Vesna

(Elsevier Science Bv, Amsterdam, 2010)

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/298
AB  - It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Regulatory Peptides
T1  - Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity
EP  - 109
IS  - 1-3
SP  - 100
VL  - 159
DO  - 10.1016/j.regpep.2009.11.002
UR  - conv_245
ER  - 
@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2010",
abstract = "It has been acknowledged that aging exerts detrimental effects on cells of the innate immune system and that neuropeptides, including neuropeptide Y (NPY) and NPY-related peptides fine-tune the activity of these cells through a receptor specific mechanism. The present study investigated the age-dependent potential of peptide YY (PYY) to modulate different granulocyte functions. The PYY reduced the carrageenan-elicited granulocyte accumulation into the air-pouch of aged (24 months) rats, and markedly decreased the phagocytosis of zymosan, as well as the H(2)O(2) production, when applied in vivo (20 mu g/air-pouch). The anti-inflammatory effect of PYY was less prominent in adult (8 months) and young (3 months) rats. However, the proportions of granulocytes expressing Y1, Y2 and Y5 receptor subtypes were significantly lower in both aged and young rats when compared to adult rats. Furthermore, the aging was found to be associated with the diminished dipeptidyl peptidase 4 (DP4, an enzyme converting the NPY and PYY to Y2/Y5 receptor selective agonists) activity in plasma. In conclusion, the diverse age-related anti-inflammatory effect of PYY in rats originates from different expression levels of Y1, Y2, and Y5 receptor subtypes in addition to different plasma DP4 activity. (C) 2009 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Regulatory Peptides",
title = "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity",
pages = "109-100",
number = "1-3",
volume = "159",
doi = "10.1016/j.regpep.2009.11.002",
url = "conv_245"
}
Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2010). Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides
Elsevier Science Bv, Amsterdam., 159(1-3), 100-109.
https://doi.org/10.1016/j.regpep.2009.11.002
conv_245
Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity. in Regulatory Peptides. 2010;159(1-3):100-109.
doi:10.1016/j.regpep.2009.11.002
conv_245 .
Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "Modulation of granulocyte functions by peptide YY in the rat: Age-related differences in Y receptors expression and plasma dipeptidyl peptidase 4 activity" in Regulatory Peptides, 159, no. 1-3 (2010):100-109,
https://doi.org/10.1016/j.regpep.2009.11.002 .,
conv_245 .
16
14
16

Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction

Mitić, Katarina; Miletić, Tatjana; Kovačević-Jovanović, Vesna; Kuštrimović, Nataša; Kosec, Duško; Dimitrijević, Mirjana; Stanojević, Stanislava

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2010)

TY  - JOUR
AU  - Mitić, Katarina
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Kuštrimović, Nataša
AU  - Kosec, Duško
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
PY  - 2010
UR  - http://intor.torlakinstitut.com/handle/123456789/311
AB  - We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity.
AB  - Ispitivan je fenotip peritonealnih ćelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne ćelije su ispitivane metodom protočne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze čestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale čestice zimozana i imale nižu sposobnost produkcije H2O2 nego ćelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do povećanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veću sposobnost fagocitoze u DA pacova prati i značajno veća zastupljenost ED1+ ćelija (koje čine uglavnom makrofage i dendritične ćelije) nasuprot većoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na ćelijama pacova AO soja. Imunizacija encefalitogenom je takođe dovela do povećanja ekspresije CD11b molekula na nerezidentnim ED2- ćelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim ćelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj različitoj osetljivosti prema indukciji autoimunskih oboljenja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction
T1  - Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova različite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).
EP  - 121
IS  - 2-3
SP  - 105
VL  - 60
DO  - 10.2298/AVB1003105M
UR  - conv_56
ER  - 
@article{
author = "Mitić, Katarina and Miletić, Tatjana and Kovačević-Jovanović, Vesna and Kuštrimović, Nataša and Kosec, Duško and Dimitrijević, Mirjana and Stanojević, Stanislava",
year = "2010",
abstract = "We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyzes and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2-macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity., Ispitivan je fenotip peritonealnih ćelija, kao i funkcije peritonealnih makrofaga, izolovanih od pacova Dark Agouti (DA) soja osetljivog na indukciju eksperimentalnog autoimunskog encefalomijelitisa (EAE) i pacova Albino Oxford (AO) soja koji je rezistentan prema EAE-u, 1, 3. i 7. dana nakon imunizacije encefalitogenom. Rezidentne peritonealne ćelije su ispitivane metodom protočne citofluorometrije, a zatim je nakon adherence testirana njihova sposobnost fagocitoze čestica zimozana i kapacitet produkcije vodonik peroksida (H2O2) i azot monoksida (NO). U neimunizovanih pacova makrofage DA soja su intenzivnije fagocitovale čestice zimozana i imale nižu sposobnost produkcije H2O2 nego ćelije pacova AO soja, ali nije bilo sojnih razlika u sposobnosti produkcije NO. Imunizacija je dovela do povećanja fagocitne sposobnosti makrofaga DA pacova, ali i do smanjenja fagocitoze i produkcije H2O2 makrofaga pacova AO soja. Generalno veću sposobnost fagocitoze u DA pacova prati i značajno veća zastupljenost ED1+ ćelija (koje čine uglavnom makrofage i dendritične ćelije) nasuprot većoj zastupljenosti ED2 antigena (marker rezidentnih makrofaga) na ćelijama pacova AO soja. Imunizacija encefalitogenom je takođe dovela do povećanja ekspresije CD11b molekula na nerezidentnim ED2- ćelijama pacova DA, ali ne i AO soja. Rane i diskretne fenotipske promene na peritonealnim ćelijama pacova oba soja verovatno odslikavaju mehanizme koji doprinose njhovoj različitoj osetljivosti prema indukciji autoimunskih oboljenja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction, Fenotipske promene izazvane imunizacijom encefalitogenom menjaju funkcije peritonealnih makrofaga u dva soja pacova različite osetljivosti prema indukciji eksperimentalnog autoimunskog encefalomijelitisa (EAE).",
pages = "121-105",
number = "2-3",
volume = "60",
doi = "10.2298/AVB1003105M",
url = "conv_56"
}
Mitić, K., Miletić, T., Kovačević-Jovanović, V., Kuštrimović, N., Kosec, D., Dimitrijević, M.,& Stanojević, S.. (2010). Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 105-121.
https://doi.org/10.2298/AVB1003105M
conv_56
Mitić K, Miletić T, Kovačević-Jovanović V, Kuštrimović N, Kosec D, Dimitrijević M, Stanojević S. Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction. in Acta veterinaria - Beograd. 2010;60(2-3):105-121.
doi:10.2298/AVB1003105M
conv_56 .
Mitić, Katarina, Miletić, Tatjana, Kovačević-Jovanović, Vesna, Kuštrimović, Nataša, Kosec, Duško, Dimitrijević, Mirjana, Stanojević, Stanislava, "Phenotype changes induced by immunization with encephalitogen affected the functions of peritoneal macrophages in two rat strains with different sensitivity to experimental autoimmune encephalomyelitis (EAE) induction" in Acta veterinaria - Beograd, 60, no. 2-3 (2010):105-121,
https://doi.org/10.2298/AVB1003105M .,
conv_56 .
1
1
1

Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria

Kovačević-Jovanović, Vesna; Mitić, Katarina; Stanojević, Stanislava; Miletić, Tatjana; Vujić, Vesna; Dimitrijević, Mirjana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2009)

TY  - JOUR
AU  - Kovačević-Jovanović, Vesna
AU  - Mitić, Katarina
AU  - Stanojević, Stanislava
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/290
AB  - The importance of commensal bacteria in the immune system development and its involvement in the etiopatogenetic mechanisms of complex multifactorial and multigenic diseases is well documented. The aim of the present study was to compare the levels of hydrogen peroxide (H2O2) and nitric oxide (NO) produced by resident peritoneal macrophages from the autoimmune disease susceptible Dark Agouti (DA) rats vs. resistant Albino Oxford (AO) rat strain, under basal conditions and subsequent to in vitro stimulation with gut commensals. Following the stimulation with phorbol myristil acetate (PMA), E. coli/PMA or P. mirabilis/PMA, AO rats macrophages have produced significantly higher levels of H2O2 compared to the cells from DA rats. Strain differences in NO production were not detected under basal conditions and after the stimulation with lipopolysaccharide and P. mirabilis. However, after the in vitro stimulation with E. coli, AO rats macrophages have produced higher levels of NO compared to DA rats macrophages. Our results demonstrated that macrophages from AO rats have higher potential to produce H2O2 and NO in response to specific commensal bacteria when compared to DA rats. A possible relationship between the macrophage activity in response to commensal bacteria and the susceptibility to induction of autoimmune/inflammatory diseases in AO and DA rat strains is suggested.
AB  - Poznato je da komensalna crevna flora ima značajnu ulogu u razvoju imunskog sistema kao i u etiopatogenezi kompleksnih multifaktorijalnih i multigenetskih bolesti. Cilj ovog rada bio je da se uporedi produkcija vodonik peroksida (H2O2) i azot monoksida (NO) peritonealnih makrofaga dva inbredna soja pacova, od kojih je jedan osetljiv (Dark Agouti, DA), a drugi rezistentan (Albino Oxford, AO) na indukciju autoimunskih bolesti, kako u bazalnim uslovima tako i nakon in vitro stimulacije makrofaga sa crevnim komensalima. Nakon stimulacije sa forbol miristil acetatom (PMA), E. coli/PMA and P. mirabilis/PMA makrofage AO pacova su produkovale značajno više H2O2 u poređenju sa makrofagama DA pacova. Nisu detektovane sojne razlike u produkciji NO u bazalnim uslovima, kao ni posle stimulacije sa lipopolisaharidom i P. mirabilis. Međutim, nakon in vitro stimulacije sa E. coli makrofage AO pacova su produkovale više NO u odnosu na makrofage DA pacova. Naši rezultati su ukazali da makrofage AO pacova imaju veći potencijal za produkciju H2O2 i NO u odgovoru na specifične komensalne bakterije. Ova različita aktivnost makrofaga može biti u vezi sa različitom osetljivošću na indukciju autoimunskih/inflamatornih bolesti kod DA i AO soja pacova.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria
T1  - Produkcija H2O2 i NO peritonealnih makrofaga pacova u odgovoru na crevne komensalne bakterije
EP  - 122
IS  - 2-3
SP  - 111
VL  - 59
DO  - 10.2298/AVB0903111K
UR  - conv_55
ER  - 
@article{
author = "Kovačević-Jovanović, Vesna and Mitić, Katarina and Stanojević, Stanislava and Miletić, Tatjana and Vujić, Vesna and Dimitrijević, Mirjana",
year = "2009",
abstract = "The importance of commensal bacteria in the immune system development and its involvement in the etiopatogenetic mechanisms of complex multifactorial and multigenic diseases is well documented. The aim of the present study was to compare the levels of hydrogen peroxide (H2O2) and nitric oxide (NO) produced by resident peritoneal macrophages from the autoimmune disease susceptible Dark Agouti (DA) rats vs. resistant Albino Oxford (AO) rat strain, under basal conditions and subsequent to in vitro stimulation with gut commensals. Following the stimulation with phorbol myristil acetate (PMA), E. coli/PMA or P. mirabilis/PMA, AO rats macrophages have produced significantly higher levels of H2O2 compared to the cells from DA rats. Strain differences in NO production were not detected under basal conditions and after the stimulation with lipopolysaccharide and P. mirabilis. However, after the in vitro stimulation with E. coli, AO rats macrophages have produced higher levels of NO compared to DA rats macrophages. Our results demonstrated that macrophages from AO rats have higher potential to produce H2O2 and NO in response to specific commensal bacteria when compared to DA rats. A possible relationship between the macrophage activity in response to commensal bacteria and the susceptibility to induction of autoimmune/inflammatory diseases in AO and DA rat strains is suggested., Poznato je da komensalna crevna flora ima značajnu ulogu u razvoju imunskog sistema kao i u etiopatogenezi kompleksnih multifaktorijalnih i multigenetskih bolesti. Cilj ovog rada bio je da se uporedi produkcija vodonik peroksida (H2O2) i azot monoksida (NO) peritonealnih makrofaga dva inbredna soja pacova, od kojih je jedan osetljiv (Dark Agouti, DA), a drugi rezistentan (Albino Oxford, AO) na indukciju autoimunskih bolesti, kako u bazalnim uslovima tako i nakon in vitro stimulacije makrofaga sa crevnim komensalima. Nakon stimulacije sa forbol miristil acetatom (PMA), E. coli/PMA and P. mirabilis/PMA makrofage AO pacova su produkovale značajno više H2O2 u poređenju sa makrofagama DA pacova. Nisu detektovane sojne razlike u produkciji NO u bazalnim uslovima, kao ni posle stimulacije sa lipopolisaharidom i P. mirabilis. Međutim, nakon in vitro stimulacije sa E. coli makrofage AO pacova su produkovale više NO u odnosu na makrofage DA pacova. Naši rezultati su ukazali da makrofage AO pacova imaju veći potencijal za produkciju H2O2 i NO u odgovoru na specifične komensalne bakterije. Ova različita aktivnost makrofaga može biti u vezi sa različitom osetljivošću na indukciju autoimunskih/inflamatornih bolesti kod DA i AO soja pacova.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria, Produkcija H2O2 i NO peritonealnih makrofaga pacova u odgovoru na crevne komensalne bakterije",
pages = "122-111",
number = "2-3",
volume = "59",
doi = "10.2298/AVB0903111K",
url = "conv_55"
}
Kovačević-Jovanović, V., Mitić, K., Stanojević, S., Miletić, T., Vujić, V.,& Dimitrijević, M.. (2009). Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(2-3), 111-122.
https://doi.org/10.2298/AVB0903111K
conv_55
Kovačević-Jovanović V, Mitić K, Stanojević S, Miletić T, Vujić V, Dimitrijević M. Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria. in Acta veterinaria - Beograd. 2009;59(2-3):111-122.
doi:10.2298/AVB0903111K
conv_55 .
Kovačević-Jovanović, Vesna, Mitić, Katarina, Stanojević, Stanislava, Miletić, Tatjana, Vujić, Vesna, Dimitrijević, Mirjana, "Production of H2O2 and NO by rat peritoneal macrophages in response to gut commensal bacteria" in Acta veterinaria - Beograd, 59, no. 2-3 (2009):111-122,
https://doi.org/10.2298/AVB0903111K .,
conv_55 .
2
2
2

The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age

Dimitrijević, Mirjana; Stanojević, Stanislava; Mitić, Katarina; Kuštrimović, Nataša; Vujić, Vesna; Miletić, Tatjana; Kovačević-Jovanović, Vesna

(Elsevier Science Inc, New York, 2008)

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Vujić, Vesna
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/243
AB  - Neuropeptide Y (NPY)-induced modulation of the immune and inflammatory responses is regulated by tissue-specific expression of different receptor subtypes (Y1-Y6) and the activity of the enzyme dipeptidyl peptidase 4 (DP4, CD26) which terminates the action of NPY on Y1 receptor subtype. The present study investigated the age-dependent effect of NPY on inflammatory paw edema and macrophage nitric oxide production in Dark Agouti rats exhibiting a high-plasma DP4 activity, as acknowledged earlier. The results showed that NPY suppressed paw edema in adult and aged, but not in young rats. Furthermore, plasma DP4 activity decreased, while macrophage DP4 activity, as well as macrophage CD26 expression increased with aging. The use of NPY-related peptides and Y receptor-specific antagonists revealed that anti-inflammatory effect of NPY is mediated via Y1 and Y5 receptors. NPY-induced suppression of paw edema in young rats following inhibition of DP4 additionally emphasized the role for Y1 receptor in the anti-inflammatory action of NPY. In contrast to the in vivo situation, NPY stimulated macrophage nitric oxide production in vitro only in young rats, and this effect was mediated via Y1 and Y2 receptors. It can be concluded that age-dependant modulation of inflammatory reactions by NPY is determined by plasma, but not macrophage DP4 activity at different ages. (c) 2008 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age
EP  - 2187
IS  - 12
SP  - 2179
VL  - 29
DO  - 10.1016/j.peptides.2008.08.017
UR  - conv_111
ER  - 
@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Mitić, Katarina and Kuštrimović, Nataša and Vujić, Vesna and Miletić, Tatjana and Kovačević-Jovanović, Vesna",
year = "2008",
abstract = "Neuropeptide Y (NPY)-induced modulation of the immune and inflammatory responses is regulated by tissue-specific expression of different receptor subtypes (Y1-Y6) and the activity of the enzyme dipeptidyl peptidase 4 (DP4, CD26) which terminates the action of NPY on Y1 receptor subtype. The present study investigated the age-dependent effect of NPY on inflammatory paw edema and macrophage nitric oxide production in Dark Agouti rats exhibiting a high-plasma DP4 activity, as acknowledged earlier. The results showed that NPY suppressed paw edema in adult and aged, but not in young rats. Furthermore, plasma DP4 activity decreased, while macrophage DP4 activity, as well as macrophage CD26 expression increased with aging. The use of NPY-related peptides and Y receptor-specific antagonists revealed that anti-inflammatory effect of NPY is mediated via Y1 and Y5 receptors. NPY-induced suppression of paw edema in young rats following inhibition of DP4 additionally emphasized the role for Y1 receptor in the anti-inflammatory action of NPY. In contrast to the in vivo situation, NPY stimulated macrophage nitric oxide production in vitro only in young rats, and this effect was mediated via Y1 and Y2 receptors. It can be concluded that age-dependant modulation of inflammatory reactions by NPY is determined by plasma, but not macrophage DP4 activity at different ages. (c) 2008 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age",
pages = "2187-2179",
number = "12",
volume = "29",
doi = "10.1016/j.peptides.2008.08.017",
url = "conv_111"
}
Dimitrijević, M., Stanojević, S., Mitić, K., Kuštrimović, N., Vujić, V., Miletić, T.,& Kovačević-Jovanović, V.. (2008). The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age. in Peptides
Elsevier Science Inc, New York., 29(12), 2179-2187.
https://doi.org/10.1016/j.peptides.2008.08.017
conv_111
Dimitrijević M, Stanojević S, Mitić K, Kuštrimović N, Vujić V, Miletić T, Kovačević-Jovanović V. The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age. in Peptides. 2008;29(12):2179-2187.
doi:10.1016/j.peptides.2008.08.017
conv_111 .
Dimitrijević, Mirjana, Stanojević, Stanislava, Mitić, Katarina, Kuštrimović, Nataša, Vujić, Vesna, Miletić, Tatjana, Kovačević-Jovanović, Vesna, "The anti-inflammatory effect of neuropeptide Y (NPY) in rats is dependent on dipeptidyl peptidase 4 (DP4) activity and age" in Peptides, 29, no. 12 (2008):2179-2187,
https://doi.org/10.1016/j.peptides.2008.08.017 .,
conv_111 .
40
37
40

Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors

Stanojević, Stanislava; Vujić, Vesna; Mitić, Katarina; Kuštrimović, Nataša; Kovačević-Jovanović, Vesna; Miletić, Tatjana; Dimitrijević, Mirjana

(Churchill Livingstone, Edinburgh, 2008)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Mitić, Katarina
AU  - Kuštrimović, Nataša
AU  - Kovačević-Jovanović, Vesna
AU  - Miletić, Tatjana
AU  - Dimitrijević, Mirjana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/259
AB  - We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.
PB  - Churchill Livingstone, Edinburgh
T2  - Neuropeptides
T1  - Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors
EP  - 158
IS  - 2
SP  - 147
VL  - 42
DO  - 10.1016/j.npep.2007.12.004
UR  - conv_206
ER  - 
@article{
author = "Stanojević, Stanislava and Vujić, Vesna and Mitić, Katarina and Kuštrimović, Nataša and Kovačević-Jovanović, Vesna and Miletić, Tatjana and Dimitrijević, Mirjana",
year = "2008",
abstract = "We investigated the involvement of specific types of opioid receptors in methionine-enkephalin (MET)-induced modulation of hydrogen peroxide (H2O2) release by rat macrophages primed with sub-optimal concentrations of phorbol myristate acetate (PMA). Peritoneal macrophages in vitro treated with different concentrations of MET were tested for H2O2 release in phenol red assay. In the antagonistic study macrophages were treated with MET and one opioid receptor antagonist, or combination of MET and two or three opioid receptor antagonists. MET decreased H2O2 release in eight individual macrophage samples, and increased it in 10 samples. The increase of H2O2 release induced by MET in macrophages was blocked with combination of opioid receptor antagonists specific delta(1,2) and mu receptors, as well as with combination of antagonists specific for delta(1,2) and kappa opioid receptors. MET-induced decrease of the H2O2 release in macrophages was prevented by opioid receptor antagonists specific for delta(1,2) or mu receptors, and also with combination of two or three opioid receptor antagonists. MET-induced enhancement of H2O2 release was mediated via delta(1) or delta(2) opioid receptor subtypes, or by mu-kappa opioid receptor functional interactions, while MET-induced suppression involved functional interactions between delta(1) and mu, delta(2) and mu, or delta(1) and kappa opioid receptors. It is possible that individual differences in basal or induced macrophage capacity to produce H2O2 might shape the repertoire of opioid receptors expression and in that way pre-determine the direction of MET-induced changes after the in vitro treatment. (c) 2007 Elsevier Ltd. All rights reserved.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Neuropeptides",
title = "Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors",
pages = "158-147",
number = "2",
volume = "42",
doi = "10.1016/j.npep.2007.12.004",
url = "conv_206"
}
Stanojević, S., Vujić, V., Mitić, K., Kuštrimović, N., Kovačević-Jovanović, V., Miletić, T.,& Dimitrijević, M.. (2008). Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors. in Neuropeptides
Churchill Livingstone, Edinburgh., 42(2), 147-158.
https://doi.org/10.1016/j.npep.2007.12.004
conv_206
Stanojević S, Vujić V, Mitić K, Kuštrimović N, Kovačević-Jovanović V, Miletić T, Dimitrijević M. Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors. in Neuropeptides. 2008;42(2):147-158.
doi:10.1016/j.npep.2007.12.004
conv_206 .
Stanojević, Stanislava, Vujić, Vesna, Mitić, Katarina, Kuštrimović, Nataša, Kovačević-Jovanović, Vesna, Miletić, Tatjana, Dimitrijević, Mirjana, "Methionine-enkephalin modulation of hydrogen peroxide (H2O2) release by rat peritoneal macrophages involves different types of opioid receptors" in Neuropeptides, 42, no. 2 (2008):147-158,
https://doi.org/10.1016/j.npep.2007.12.004 .,
conv_206 .
18
19
18

The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress

Stanojević, Stanislava; Kuštrimović, Nataša; Mitić, Katarina; Miletić, Tatjana; Vujić, Vesna; Kovačević-Jovanović, Vesna; Dimitrijević, Mirjana

(Karger, Basel, 2008)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Kuštrimović, Nataša
AU  - Mitić, Katarina
AU  - Miletić, Tatjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/260
AB  - Background: Given that stressful experiences can change the reaction to a subsequent exposure to stress, we tested the in vitro effects of the stress mediator corticosterone and the opioid peptide beta-endorphin on the function of macrophages isolated from control rats and from rats exposed to electric tail shock stress (ES) or a stress-witnessing procedure (SW) 24 h earlier. Methods: Peritoneal macrophages isolated from control and stressed rats of the Dark Agouti (DA) strain were treated in vitro with corticosterone or beta-endorphin and tested for adherence, phagocytosis and hydrogen peroxide release. Results: ES diminished adherence and SW decreased phagocytosis. The suppressive effect of corticosterone on phagocytosis was absent in rats exposed to ES and SW, while the suppressive effect of beta-endorphin on adherence was not observed in rats exposed to SW. ES and SW did not affect H2O2 release, neither directly nor indirectly by changing macrophage response to corticosterone and beta-endorphin in this test. Conclusions: In DA rats early macrophage activation steps, i.e. adherence and phagocytosis, were more sensitive to stress than their effector function, corresponding to H2O2 production. We suggest that neuroendocrine mediators of stress that converge on macrophages might have changed specific macrophage receptors or postreceptor events and alter their response to artificial stressors, represented by corticosterone and beta-endorphin in vitro. Copyright (C) 2008 S. Karger AG, Basel.
PB  - Karger, Basel
T2  - Neuroimmunomodulation
T1  - The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress
EP  - 116
IS  - 2
SP  - 108
VL  - 15
DO  - 10.1159/000148193
UR  - conv_220
ER  - 
@article{
author = "Stanojević, Stanislava and Kuštrimović, Nataša and Mitić, Katarina and Miletić, Tatjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana",
year = "2008",
abstract = "Background: Given that stressful experiences can change the reaction to a subsequent exposure to stress, we tested the in vitro effects of the stress mediator corticosterone and the opioid peptide beta-endorphin on the function of macrophages isolated from control rats and from rats exposed to electric tail shock stress (ES) or a stress-witnessing procedure (SW) 24 h earlier. Methods: Peritoneal macrophages isolated from control and stressed rats of the Dark Agouti (DA) strain were treated in vitro with corticosterone or beta-endorphin and tested for adherence, phagocytosis and hydrogen peroxide release. Results: ES diminished adherence and SW decreased phagocytosis. The suppressive effect of corticosterone on phagocytosis was absent in rats exposed to ES and SW, while the suppressive effect of beta-endorphin on adherence was not observed in rats exposed to SW. ES and SW did not affect H2O2 release, neither directly nor indirectly by changing macrophage response to corticosterone and beta-endorphin in this test. Conclusions: In DA rats early macrophage activation steps, i.e. adherence and phagocytosis, were more sensitive to stress than their effector function, corresponding to H2O2 production. We suggest that neuroendocrine mediators of stress that converge on macrophages might have changed specific macrophage receptors or postreceptor events and alter their response to artificial stressors, represented by corticosterone and beta-endorphin in vitro. Copyright (C) 2008 S. Karger AG, Basel.",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress",
pages = "116-108",
number = "2",
volume = "15",
doi = "10.1159/000148193",
url = "conv_220"
}
Stanojević, S., Kuštrimović, N., Mitić, K., Miletić, T., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2008). The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress. in Neuroimmunomodulation
Karger, Basel., 15(2), 108-116.
https://doi.org/10.1159/000148193
conv_220
Stanojević S, Kuštrimović N, Mitić K, Miletić T, Vujić V, Kovačević-Jovanović V, Dimitrijević M. The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress. in Neuroimmunomodulation. 2008;15(2):108-116.
doi:10.1159/000148193
conv_220 .
Stanojević, Stanislava, Kuštrimović, Nataša, Mitić, Katarina, Miletić, Tatjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "The effects of corticosterone and beta-endorphin on adherence, phagocytosis and hydrogen peroxide production of macrophages isolated from Dark Agouti rats exposed to acute stress" in Neuroimmunomodulation, 15, no. 2 (2008):108-116,
https://doi.org/10.1159/000148193 .,
conv_220 .
8
9
9

The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains

Stanojević, Stanislava; Mitić, Katarina; Vujić, Vesna; Kovačević-Jovanović, Vesna; Dimitrijević, Mirjana

(Pergamon-Elsevier Science Ltd, Oxford, 2007)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/228
AB  - The aim of our current study was to investigate the effect of acute exposure to electric tail shock stress (ES) and to a stress witnessing procedure (SW), as models for physical and psychological stress paradigms, respectively, on phagocytosis and H2O2 production in peritoneal macrophages isolated from Albino Oxford (AO) and Dark Agouti (DA) rats. In addition, we studied the in vitro effects of methionine-enkephalin (ME) on phagocytosis and H2O2 production in peritoneal macrophages isolated from both AO and DA rats that had been exposed to ES and SW procedures. The results showed that peritoneal macrophages isolated from DA rats were less sensitive to the suppressive effects of ES and SW than macrophages isolated from AO rats. In vitro treatment of macrophages isolated from AO rats with ME mimicked to some extent the suppressive effects of ES and SW on phagocytosis and H2O2 production and additionally diminished H2O2 release in macrophages isolated from AO rats previously exposed to ES or SW ME did not have any effect on phagocytosis in macrophages isolated from DA rats, but changed H2O2 production in a concentration-dependent manner. In macrophages isolated from DA rats previously exposed to stress the effect of ME was dependent on the macrophage function tested and the particular stress paradigm employed. Our results emphasise the fact that both beneficial and detrimental effects of stress on immune system functions could be attributed to the individual variations in the macrophage's response to stress mediators. (c) 2006 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains
EP  - 909
IS  - 10
SP  - 901
VL  - 80
DO  - 10.1016/j.lfs.2006.11.019
UR  - conv_190
ER  - 
@article{
author = "Stanojević, Stanislava and Mitić, Katarina and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana",
year = "2007",
abstract = "The aim of our current study was to investigate the effect of acute exposure to electric tail shock stress (ES) and to a stress witnessing procedure (SW), as models for physical and psychological stress paradigms, respectively, on phagocytosis and H2O2 production in peritoneal macrophages isolated from Albino Oxford (AO) and Dark Agouti (DA) rats. In addition, we studied the in vitro effects of methionine-enkephalin (ME) on phagocytosis and H2O2 production in peritoneal macrophages isolated from both AO and DA rats that had been exposed to ES and SW procedures. The results showed that peritoneal macrophages isolated from DA rats were less sensitive to the suppressive effects of ES and SW than macrophages isolated from AO rats. In vitro treatment of macrophages isolated from AO rats with ME mimicked to some extent the suppressive effects of ES and SW on phagocytosis and H2O2 production and additionally diminished H2O2 release in macrophages isolated from AO rats previously exposed to ES or SW ME did not have any effect on phagocytosis in macrophages isolated from DA rats, but changed H2O2 production in a concentration-dependent manner. In macrophages isolated from DA rats previously exposed to stress the effect of ME was dependent on the macrophage function tested and the particular stress paradigm employed. Our results emphasise the fact that both beneficial and detrimental effects of stress on immune system functions could be attributed to the individual variations in the macrophage's response to stress mediators. (c) 2006 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains",
pages = "909-901",
number = "10",
volume = "80",
doi = "10.1016/j.lfs.2006.11.019",
url = "conv_190"
}
Stanojević, S., Mitić, K., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2007). The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 80(10), 901-909.
https://doi.org/10.1016/j.lfs.2006.11.019
conv_190
Stanojević S, Mitić K, Vujić V, Kovačević-Jovanović V, Dimitrijević M. The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains. in Life Sciences. 2007;80(10):901-909.
doi:10.1016/j.lfs.2006.11.019
conv_190 .
Stanojević, Stanislava, Mitić, Katarina, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "The influence of stress and methionine-enkephalin on macrophage functions in two inbred rat strains" in Life Sciences, 80, no. 10 (2007):901-909,
https://doi.org/10.1016/j.lfs.2006.11.019 .,
conv_190 .
3
17
17
18

Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats

Miletić, Tatjana; Kovačević-Jovanović, Vesna; Vujić, Vesna; Stanojević, Stanislava; Mitić, Katarina; Lazarević-Macanović, Miriana; Dimitrijević, Mirjana

(Elsevier Gmbh, Munich, 2007)

TY  - JOUR
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Vujić, Vesna
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Lazarević-Macanović, Miriana
AU  - Dimitrijević, Mirjana
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/229
AB  - There is extensive evidence for the critical role of reactive oxygen species (ROS) and nitric oxide (NO) produced by phagocytes in development of inflammatory processes and pathogenesis of numerous diseases, including rheumatoid arthritis (RA). Apart from their function as mediators of inflammation and tissue damage, recent research supports their role as signaling and regulatory molecules. In the present study we have investigated the production of ROS and NO over the course of adjuvant arthritis (AA) and oil-induced arthritis (OIA), by resident peritoneal macrophages of two rat strains: Dark Agouti (DA), susceptible, and Albino Oxford (AO), resistant to induction of AA and OIA. We have compared levels of ROS and NO produced by susceptible vs. resistant rat strain, and investigated their relevancy for arthritis development and severity. In addition, we have stimulated macrophages in vitro with Mycobacterium bovis BCG, and two heat shock proteins (HSP): endogenous HSP47 and mycobacterial HSP71 (rnHSP71). Our results suggest a possible contribution of increased ROS production to arthritis resistance of AO rats. The ROS production in AO rats is potentiated by endogenous HSP47, but not with mycobacterial cell and mHSP71, suggesting HSP47 participates in AA control. We have found no fundamental relationship between the magnitude of NO production and AA and OIA susceptibility and severity, suggesting that NO has no effector role in AA and OIA. Our results advocate a regulatory type action of NO molecule aught be more significant in arthritis development. (c) 2006 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Munich
T2  - Immunobiology
T1  - Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats
EP  - 105
IS  - 2
SP  - 95
VL  - 212
DO  - 10.1016/j.imbio.2006.11.012
UR  - conv_193
ER  - 
@article{
author = "Miletić, Tatjana and Kovačević-Jovanović, Vesna and Vujić, Vesna and Stanojević, Stanislava and Mitić, Katarina and Lazarević-Macanović, Miriana and Dimitrijević, Mirjana",
year = "2007",
abstract = "There is extensive evidence for the critical role of reactive oxygen species (ROS) and nitric oxide (NO) produced by phagocytes in development of inflammatory processes and pathogenesis of numerous diseases, including rheumatoid arthritis (RA). Apart from their function as mediators of inflammation and tissue damage, recent research supports their role as signaling and regulatory molecules. In the present study we have investigated the production of ROS and NO over the course of adjuvant arthritis (AA) and oil-induced arthritis (OIA), by resident peritoneal macrophages of two rat strains: Dark Agouti (DA), susceptible, and Albino Oxford (AO), resistant to induction of AA and OIA. We have compared levels of ROS and NO produced by susceptible vs. resistant rat strain, and investigated their relevancy for arthritis development and severity. In addition, we have stimulated macrophages in vitro with Mycobacterium bovis BCG, and two heat shock proteins (HSP): endogenous HSP47 and mycobacterial HSP71 (rnHSP71). Our results suggest a possible contribution of increased ROS production to arthritis resistance of AO rats. The ROS production in AO rats is potentiated by endogenous HSP47, but not with mycobacterial cell and mHSP71, suggesting HSP47 participates in AA control. We have found no fundamental relationship between the magnitude of NO production and AA and OIA susceptibility and severity, suggesting that NO has no effector role in AA and OIA. Our results advocate a regulatory type action of NO molecule aught be more significant in arthritis development. (c) 2006 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Munich",
journal = "Immunobiology",
title = "Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats",
pages = "105-95",
number = "2",
volume = "212",
doi = "10.1016/j.imbio.2006.11.012",
url = "conv_193"
}
Miletić, T., Kovačević-Jovanović, V., Vujić, V., Stanojević, S., Mitić, K., Lazarević-Macanović, M.,& Dimitrijević, M.. (2007). Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats. in Immunobiology
Elsevier Gmbh, Munich., 212(2), 95-105.
https://doi.org/10.1016/j.imbio.2006.11.012
conv_193
Miletić T, Kovačević-Jovanović V, Vujić V, Stanojević S, Mitić K, Lazarević-Macanović M, Dimitrijević M. Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats. in Immunobiology. 2007;212(2):95-105.
doi:10.1016/j.imbio.2006.11.012
conv_193 .
Miletić, Tatjana, Kovačević-Jovanović, Vesna, Vujić, Vesna, Stanojević, Stanislava, Mitić, Katarina, Lazarević-Macanović, Miriana, Dimitrijević, Mirjana, "Reactive oxygen species (ROS), but not nitric oxide (NO), contribute to strain differences in the susceptibility to experimental arthritis in rats" in Immunobiology, 212, no. 2 (2007):95-105,
https://doi.org/10.1016/j.imbio.2006.11.012 .,
conv_193 .
14
14
15

Exposure to acute physical and psychological stress alters the response of rat macrophages to corticosterone, neuropeptide Y and beta-endorphin

Stanojević, Stanislava; Mitić, Katarina; Vujić, Vesna; Kovačević-Jovanović, Vesna; Dimitrijević, Mirjana

(Taylor & Francis Ltd, Abingdon, 2007)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/238
AB  - The objective of the present study was to investigate the effect of acute exposure to electric tail shock stress (ES) and a stress witnessing procedure ( SW), as models for physical and psychological stress paradigms, respectively on adherence, phagocytosis and hydrogen peroxide (H2O2) release from rat peritoneal macrophages. In addition, we studied the in vitro effects of corticosterone (CORT), neuropeptide Y (NPY) and beta-endorphin (BE) on adherence, phagocytosis and H2O2 release from macrophages isolated from control rats and from rats that had been exposed to ES or SW procedures 24 h earlier. ES and SW comparably diminished phagocytosis and H2O2 release, but did not influence macrophage adherence. In vitro treatment with CORT and NPY notably suppressed phagocytosis and potentiated H2O2 release from macrophages. BE suppressed both phagocytosis and H2O2 release from macrophages. Previous exposure to ES and SW altered the responsiveness of the isolated macrophages to their in vitro treatment with mediators of stress, making the cells less sensitive to the influence of CORT and NPY and to a lesser extent to BE. It could be concluded that changes in the local macrophage milieu induced by ES and SW 24 h earlier modify macrophage responses to subsequent in vitro exposure to the stress mimics, CORT, NPY and BE.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Stress-The International Journal on the Biology of Stress
T1  - Exposure to acute physical and psychological stress alters the response of rat macrophages to corticosterone, neuropeptide Y and beta-endorphin
EP  - 73
IS  - 1
SP  - 65
VL  - 10
DO  - 10.1080/10253890601181289
UR  - conv_195
ER  - 
@article{
author = "Stanojević, Stanislava and Mitić, Katarina and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana",
year = "2007",
abstract = "The objective of the present study was to investigate the effect of acute exposure to electric tail shock stress (ES) and a stress witnessing procedure ( SW), as models for physical and psychological stress paradigms, respectively on adherence, phagocytosis and hydrogen peroxide (H2O2) release from rat peritoneal macrophages. In addition, we studied the in vitro effects of corticosterone (CORT), neuropeptide Y (NPY) and beta-endorphin (BE) on adherence, phagocytosis and H2O2 release from macrophages isolated from control rats and from rats that had been exposed to ES or SW procedures 24 h earlier. ES and SW comparably diminished phagocytosis and H2O2 release, but did not influence macrophage adherence. In vitro treatment with CORT and NPY notably suppressed phagocytosis and potentiated H2O2 release from macrophages. BE suppressed both phagocytosis and H2O2 release from macrophages. Previous exposure to ES and SW altered the responsiveness of the isolated macrophages to their in vitro treatment with mediators of stress, making the cells less sensitive to the influence of CORT and NPY and to a lesser extent to BE. It could be concluded that changes in the local macrophage milieu induced by ES and SW 24 h earlier modify macrophage responses to subsequent in vitro exposure to the stress mimics, CORT, NPY and BE.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Stress-The International Journal on the Biology of Stress",
title = "Exposure to acute physical and psychological stress alters the response of rat macrophages to corticosterone, neuropeptide Y and beta-endorphin",
pages = "73-65",
number = "1",
volume = "10",
doi = "10.1080/10253890601181289",
url = "conv_195"
}
Stanojević, S., Mitić, K., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2007). Exposure to acute physical and psychological stress alters the response of rat macrophages to corticosterone, neuropeptide Y and beta-endorphin. in Stress-The International Journal on the Biology of Stress
Taylor & Francis Ltd, Abingdon., 10(1), 65-73.
https://doi.org/10.1080/10253890601181289
conv_195
Stanojević S, Mitić K, Vujić V, Kovačević-Jovanović V, Dimitrijević M. Exposure to acute physical and psychological stress alters the response of rat macrophages to corticosterone, neuropeptide Y and beta-endorphin. in Stress-The International Journal on the Biology of Stress. 2007;10(1):65-73.
doi:10.1080/10253890601181289
conv_195 .
Stanojević, Stanislava, Mitić, Katarina, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "Exposure to acute physical and psychological stress alters the response of rat macrophages to corticosterone, neuropeptide Y and beta-endorphin" in Stress-The International Journal on the Biology of Stress, 10, no. 1 (2007):65-73,
https://doi.org/10.1080/10253890601181289 .,
conv_195 .
11
12
13

Age-related effect of peptide YY (PYY) on paw edema in the rat: The function of Y1 receptors on inflammatory cells

Stanojević, Stanislava; Vujić, Vesna; Kovačević-Jovanović, Vesna; Mitić, Katarina; Kosec, Duško; von Hoersten, Stephan; Dimitrijević, Mirjana

(Pergamon-Elsevier Science Ltd, Oxford, 2006)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Mitić, Katarina
AU  - Kosec, Duško
AU  - von Hoersten, Stephan
AU  - Dimitrijević, Mirjana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/211
AB  - It is well documented that neuropeptides participate in local inflammatory reaction and modulate functions of inflammatory cells. The aim of the study was to determine a link between in vivo and in vitro effects of NPY-related peptides on inflammatory response with respect to ageing. Peptide YY (PYY) intraplantarly applied decreases concanavalin A-induced paw edema in 3 and 8 months, but not in 24 months old male rats of Albino Oxford strain. The use of NPY-related receptor-specific peptides and Y1 receptor antagonist revealed that anti-inflammatory effect of PYY is mediated via NPY Y1 receptors. PYY in vitro decreases adherence of macrophages from 8 months, but not from 3 and 24 months old rats and this effect is also mediated via NPY Y1 receptor. Additionally, PYY (10(-6) M) decreases NBT reduction in macrophages from 3 and 8 months old rats, and suppresses NO production in cells from 24 months old rats, albeit regardless of absence of in vivo effect of PYY on inflammation in aged rats. It is concluded that aged rats are less responsive to anti-inflammatory action of PYY compared to adult and young rats, and that ageing is associated with altered NPY Y1 receptor functioning. (c) 2006 Elsevier Inc. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - Age-related effect of peptide YY (PYY) on paw edema in the rat: The function of Y1 receptors on inflammatory cells
EP  - 799
IS  - 8
SP  - 793
VL  - 41
DO  - 10.1016/j.exger.2006.05.012
UR  - conv_182
ER  - 
@article{
author = "Stanojević, Stanislava and Vujić, Vesna and Kovačević-Jovanović, Vesna and Mitić, Katarina and Kosec, Duško and von Hoersten, Stephan and Dimitrijević, Mirjana",
year = "2006",
abstract = "It is well documented that neuropeptides participate in local inflammatory reaction and modulate functions of inflammatory cells. The aim of the study was to determine a link between in vivo and in vitro effects of NPY-related peptides on inflammatory response with respect to ageing. Peptide YY (PYY) intraplantarly applied decreases concanavalin A-induced paw edema in 3 and 8 months, but not in 24 months old male rats of Albino Oxford strain. The use of NPY-related receptor-specific peptides and Y1 receptor antagonist revealed that anti-inflammatory effect of PYY is mediated via NPY Y1 receptors. PYY in vitro decreases adherence of macrophages from 8 months, but not from 3 and 24 months old rats and this effect is also mediated via NPY Y1 receptor. Additionally, PYY (10(-6) M) decreases NBT reduction in macrophages from 3 and 8 months old rats, and suppresses NO production in cells from 24 months old rats, albeit regardless of absence of in vivo effect of PYY on inflammation in aged rats. It is concluded that aged rats are less responsive to anti-inflammatory action of PYY compared to adult and young rats, and that ageing is associated with altered NPY Y1 receptor functioning. (c) 2006 Elsevier Inc. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "Age-related effect of peptide YY (PYY) on paw edema in the rat: The function of Y1 receptors on inflammatory cells",
pages = "799-793",
number = "8",
volume = "41",
doi = "10.1016/j.exger.2006.05.012",
url = "conv_182"
}
Stanojević, S., Vujić, V., Kovačević-Jovanović, V., Mitić, K., Kosec, D., von Hoersten, S.,& Dimitrijević, M.. (2006). Age-related effect of peptide YY (PYY) on paw edema in the rat: The function of Y1 receptors on inflammatory cells. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 41(8), 793-799.
https://doi.org/10.1016/j.exger.2006.05.012
conv_182
Stanojević S, Vujić V, Kovačević-Jovanović V, Mitić K, Kosec D, von Hoersten S, Dimitrijević M. Age-related effect of peptide YY (PYY) on paw edema in the rat: The function of Y1 receptors on inflammatory cells. in Experimental Gerontology. 2006;41(8):793-799.
doi:10.1016/j.exger.2006.05.012
conv_182 .
Stanojević, Stanislava, Vujić, Vesna, Kovačević-Jovanović, Vesna, Mitić, Katarina, Kosec, Duško, von Hoersten, Stephan, Dimitrijević, Mirjana, "Age-related effect of peptide YY (PYY) on paw edema in the rat: The function of Y1 receptors on inflammatory cells" in Experimental Gerontology, 41, no. 8 (2006):793-799,
https://doi.org/10.1016/j.exger.2006.05.012 .,
conv_182 .
16
17
17

beta-endorphin differentially affects inflammation in two inbred rat strains

Stanojević, Stanislava; Mitić, Katarina; Vujić, Vesna; Kovačević-Jovanović, Vesna; Dimitrijević, Mirjana

(Elsevier, Amsterdam, 2006)

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Mitić, Katarina
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/212
AB  - It has been shown that inflammation of rat paws elicits accumulation of opioid peptide is-endorphin-containing immune cells in the inflamed subcutaneous tissue, contributing to immumocyte-produced pain suppression. However, the possible mechanisms involved in the pharmacological application of beta-endorphin in rat paw inflammation have not been investigated. The present study was set up to explore the effects of intraplantar injection of beta-endorphin on Concanavalin A-induced paw edema in two inbred rat strains, Albino Oxford (AO) and Dark Agouti (DA). Both high dose-induced suppression and low dose-induced potentiation of edema development in AO and DA rats, respectively, were blocked with antagonists specific for 6 (naltrindole) and K (nor-binaltorphimine) opioid receptors. beta-endorphin in vitro decreased phagocytosis and increased nitric oxide (NO) production in air pouch granulocytes obtained from AD rats. However, in cells from DA rat strain beta-endorphin modulated both phagocytosis and NO production in a concentration-dependent manner. It could be concluded that the strain-dependent opposing effects of endorphin on paw inflammation are mediated through 6 and K opioid receptors and probably involve changes in the production of reactive oxygen species by inflammatory cells. Our results point to the importance of genotype for pharmacological manipulations and the development of inflammation. (c) 2006 Elsevier B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - European Journal of Pharmacology
T1  - beta-endorphin differentially affects inflammation in two inbred rat strains
EP  - 165
IS  - 1-3
SP  - 157
VL  - 549
DO  - 10.1016/j.ejphar.2006.08.012
UR  - conv_183
ER  - 
@article{
author = "Stanojević, Stanislava and Mitić, Katarina and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana",
year = "2006",
abstract = "It has been shown that inflammation of rat paws elicits accumulation of opioid peptide is-endorphin-containing immune cells in the inflamed subcutaneous tissue, contributing to immumocyte-produced pain suppression. However, the possible mechanisms involved in the pharmacological application of beta-endorphin in rat paw inflammation have not been investigated. The present study was set up to explore the effects of intraplantar injection of beta-endorphin on Concanavalin A-induced paw edema in two inbred rat strains, Albino Oxford (AO) and Dark Agouti (DA). Both high dose-induced suppression and low dose-induced potentiation of edema development in AO and DA rats, respectively, were blocked with antagonists specific for 6 (naltrindole) and K (nor-binaltorphimine) opioid receptors. beta-endorphin in vitro decreased phagocytosis and increased nitric oxide (NO) production in air pouch granulocytes obtained from AD rats. However, in cells from DA rat strain beta-endorphin modulated both phagocytosis and NO production in a concentration-dependent manner. It could be concluded that the strain-dependent opposing effects of endorphin on paw inflammation are mediated through 6 and K opioid receptors and probably involve changes in the production of reactive oxygen species by inflammatory cells. Our results point to the importance of genotype for pharmacological manipulations and the development of inflammation. (c) 2006 Elsevier B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "European Journal of Pharmacology",
title = "beta-endorphin differentially affects inflammation in two inbred rat strains",
pages = "165-157",
number = "1-3",
volume = "549",
doi = "10.1016/j.ejphar.2006.08.012",
url = "conv_183"
}
Stanojević, S., Mitić, K., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2006). beta-endorphin differentially affects inflammation in two inbred rat strains. in European Journal of Pharmacology
Elsevier, Amsterdam., 549(1-3), 157-165.
https://doi.org/10.1016/j.ejphar.2006.08.012
conv_183
Stanojević S, Mitić K, Vujić V, Kovačević-Jovanović V, Dimitrijević M. beta-endorphin differentially affects inflammation in two inbred rat strains. in European Journal of Pharmacology. 2006;549(1-3):157-165.
doi:10.1016/j.ejphar.2006.08.012
conv_183 .
Stanojević, Stanislava, Mitić, Katarina, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "beta-endorphin differentially affects inflammation in two inbred rat strains" in European Journal of Pharmacology, 549, no. 1-3 (2006):157-165,
https://doi.org/10.1016/j.ejphar.2006.08.012 .,
conv_183 .
14
15
16

Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats

Miletić, Tatjana; Kovačević-Jovanović, Vesna; Stanojević, Stanislava; Vujić, Vesna; Kosec, Duško; Mitić, Katarina; Dimitrijević, Mirjana

(Wiley, Hoboken, 2006)

TY  - JOUR
AU  - Miletić, Tatjana
AU  - Kovačević-Jovanović, Vesna
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Kosec, Duško
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/213
AB  - Because of high sequence homology between microbial and endogenous heat shock proteins (HSP), immunological cross-reactivity to microbial HSP has been suggested as a possible cause of the development of autoimmune diseases, such as rheumatoid arthritis. The present study aimed to determine a potential role of HSP47, a molecular chaperone involved in the synthesis and assembly of collagen molecules, and microbial HSP71 (mHSP71) in adjuvant arthritis (AA) in two rat strains: Dark Agouti (DA), susceptible to AA induction and Albino Oxford (AO), which is resistant to AA induction. Immunization with complete Freund's adjuvant (CFA) induced an increased expression of HSP47 in joints of DA rats, which exhibited severe clinical signs of AA at the time of disease peak, while this protein was not detectable in joints of AO rats. In contrast, no strain differences in HSP72 (rat analogue of mHSP71) expressions in joints were observed. The increased levels of anti-HSP47 antibodies were detected in sera of DA rats during the AA peak, while the immunization with CFA increased levels of anti-mHSP71 antibodies in sera of AO rats. HSP47 and mHSP71 reduced proliferation of draining inguinal lymph node cells (LNC) in resistant AO rat strain, leading to a hypothesis that both HSP participated in AA control. Finally, mHSP71 potentiated the apoptotic response of LNC in susceptible DA rat strain. In conclusion, our findings indicate involvement of HSP47 in the development of AA in the rat, and point out to the regulatory role for both HSP47 and mHSP71.
PB  - Wiley, Hoboken
T2  - Scandinavian Journal of Immunology
T1  - Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats
EP  - 632
IS  - 6
SP  - 623
VL  - 64
DO  - 10.1111/j.1365-3083.2006.01852.x
UR  - conv_184
ER  - 
@article{
author = "Miletić, Tatjana and Kovačević-Jovanović, Vesna and Stanojević, Stanislava and Vujić, Vesna and Kosec, Duško and Mitić, Katarina and Dimitrijević, Mirjana",
year = "2006",
abstract = "Because of high sequence homology between microbial and endogenous heat shock proteins (HSP), immunological cross-reactivity to microbial HSP has been suggested as a possible cause of the development of autoimmune diseases, such as rheumatoid arthritis. The present study aimed to determine a potential role of HSP47, a molecular chaperone involved in the synthesis and assembly of collagen molecules, and microbial HSP71 (mHSP71) in adjuvant arthritis (AA) in two rat strains: Dark Agouti (DA), susceptible to AA induction and Albino Oxford (AO), which is resistant to AA induction. Immunization with complete Freund's adjuvant (CFA) induced an increased expression of HSP47 in joints of DA rats, which exhibited severe clinical signs of AA at the time of disease peak, while this protein was not detectable in joints of AO rats. In contrast, no strain differences in HSP72 (rat analogue of mHSP71) expressions in joints were observed. The increased levels of anti-HSP47 antibodies were detected in sera of DA rats during the AA peak, while the immunization with CFA increased levels of anti-mHSP71 antibodies in sera of AO rats. HSP47 and mHSP71 reduced proliferation of draining inguinal lymph node cells (LNC) in resistant AO rat strain, leading to a hypothesis that both HSP participated in AA control. Finally, mHSP71 potentiated the apoptotic response of LNC in susceptible DA rat strain. In conclusion, our findings indicate involvement of HSP47 in the development of AA in the rat, and point out to the regulatory role for both HSP47 and mHSP71.",
publisher = "Wiley, Hoboken",
journal = "Scandinavian Journal of Immunology",
title = "Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats",
pages = "632-623",
number = "6",
volume = "64",
doi = "10.1111/j.1365-3083.2006.01852.x",
url = "conv_184"
}
Miletić, T., Kovačević-Jovanović, V., Stanojević, S., Vujić, V., Kosec, D., Mitić, K.,& Dimitrijević, M.. (2006). Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats. in Scandinavian Journal of Immunology
Wiley, Hoboken., 64(6), 623-632.
https://doi.org/10.1111/j.1365-3083.2006.01852.x
conv_184
Miletić T, Kovačević-Jovanović V, Stanojević S, Vujić V, Kosec D, Mitić K, Dimitrijević M. Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats. in Scandinavian Journal of Immunology. 2006;64(6):623-632.
doi:10.1111/j.1365-3083.2006.01852.x
conv_184 .
Miletić, Tatjana, Kovačević-Jovanović, Vesna, Stanojević, Stanislava, Vujić, Vesna, Kosec, Duško, Mitić, Katarina, Dimitrijević, Mirjana, "Strain differences and the role for HSP47 and HSP70 in adjuvant arthritis in rats" in Scandinavian Journal of Immunology, 64, no. 6 (2006):623-632,
https://doi.org/10.1111/j.1365-3083.2006.01852.x .,
conv_184 .
10
9
10

Neuropeptide Y (NPY) modulates oxidative burst and nitric oxide production in carrageenan-elicited granulocytes from rat air pouch

Dimitrijević, Mirjana; Stanojević, Stanislava; Micić, Stana; Vujić, Vesna; Kovačević-Jovanović, Vesna; Mitić, Katarina; von Hoersten, Stephan; Kosec, Duško

(Elsevier Science Inc, New York, 2006)

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Stanojević, Stanislava
AU  - Micić, Stana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Mitić, Katarina
AU  - von Hoersten, Stephan
AU  - Kosec, Duško
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/218
AB  - We studied the effects of neuropeptide Y (NPY) and NPY-related receptor specific peptides on functions of carrageenan-elicited granulocytes in vitro and ability of NPY to modulate carrageenan-induced air pouch inflammation in rats in vivo. Anti-inflammatory effect of NPY comprises reduced granulocyte accumulation into the air pouch, to some extent attenuation of phagocytosis, attained via Y1 receptor, and considerable decrease in peroxide production, albeit mediated via Y2 and Y5 receptors activation. Conversely, NPY increases nitric oxide production and this potentiation is mediated via Y1 receptor. It is concluded that NPY Y1 and Y2/Y5 receptors' interaction participates in NPY-induced modulation of granulocyte functions related to inflammation. (c) 2006 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - Neuropeptide Y (NPY) modulates oxidative burst and nitric oxide production in carrageenan-elicited granulocytes from rat air pouch
EP  - 3215
IS  - 12
SP  - 3208
VL  - 27
DO  - 10.1016/j.peptides.2006.08.018
UR  - conv_109
ER  - 
@article{
author = "Dimitrijević, Mirjana and Stanojević, Stanislava and Micić, Stana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Mitić, Katarina and von Hoersten, Stephan and Kosec, Duško",
year = "2006",
abstract = "We studied the effects of neuropeptide Y (NPY) and NPY-related receptor specific peptides on functions of carrageenan-elicited granulocytes in vitro and ability of NPY to modulate carrageenan-induced air pouch inflammation in rats in vivo. Anti-inflammatory effect of NPY comprises reduced granulocyte accumulation into the air pouch, to some extent attenuation of phagocytosis, attained via Y1 receptor, and considerable decrease in peroxide production, albeit mediated via Y2 and Y5 receptors activation. Conversely, NPY increases nitric oxide production and this potentiation is mediated via Y1 receptor. It is concluded that NPY Y1 and Y2/Y5 receptors' interaction participates in NPY-induced modulation of granulocyte functions related to inflammation. (c) 2006 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "Neuropeptide Y (NPY) modulates oxidative burst and nitric oxide production in carrageenan-elicited granulocytes from rat air pouch",
pages = "3215-3208",
number = "12",
volume = "27",
doi = "10.1016/j.peptides.2006.08.018",
url = "conv_109"
}
Dimitrijević, M., Stanojević, S., Micić, S., Vujić, V., Kovačević-Jovanović, V., Mitić, K., von Hoersten, S.,& Kosec, D.. (2006). Neuropeptide Y (NPY) modulates oxidative burst and nitric oxide production in carrageenan-elicited granulocytes from rat air pouch. in Peptides
Elsevier Science Inc, New York., 27(12), 3208-3215.
https://doi.org/10.1016/j.peptides.2006.08.018
conv_109
Dimitrijević M, Stanojević S, Micić S, Vujić V, Kovačević-Jovanović V, Mitić K, von Hoersten S, Kosec D. Neuropeptide Y (NPY) modulates oxidative burst and nitric oxide production in carrageenan-elicited granulocytes from rat air pouch. in Peptides. 2006;27(12):3208-3215.
doi:10.1016/j.peptides.2006.08.018
conv_109 .
Dimitrijević, Mirjana, Stanojević, Stanislava, Micić, Stana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Mitić, Katarina, von Hoersten, Stephan, Kosec, Duško, "Neuropeptide Y (NPY) modulates oxidative burst and nitric oxide production in carrageenan-elicited granulocytes from rat air pouch" in Peptides, 27, no. 12 (2006):3208-3215,
https://doi.org/10.1016/j.peptides.2006.08.018 .,
conv_109 .
27
26
28

Age-related effect of peptide YY(PYY) on paw edema in rat: The function of Y1 receptors on inflammatory

Stanojević, Stanislava; Vujić, Vesna; Kovačević-Jovanović, Vesna; Mitić, Katarina; Kosec, Duško; von Hoersten, Stephan; Dimitrijević, Mirjana

(Savez farmaceutskih udruženja Srbije, Beograd, 2006)

TY  - CONF
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Mitić, Katarina
AU  - Kosec, Duško
AU  - von Hoersten, Stephan
AU  - Dimitrijević, Mirjana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/222
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Age-related effect of peptide YY(PYY) on paw edema in rat: The function of Y1 receptors on inflammatory
T1  - Efekat peptida YY(PYY) na edem šape pacova u starenju - uloga Y1 receptora na inflamatornim ćelijama
EP  - 401
IS  - 4
SP  - 400
VL  - 56
UR  - conv_3
ER  - 
@conference{
author = "Stanojević, Stanislava and Vujić, Vesna and Kovačević-Jovanović, Vesna and Mitić, Katarina and Kosec, Duško and von Hoersten, Stephan and Dimitrijević, Mirjana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Age-related effect of peptide YY(PYY) on paw edema in rat: The function of Y1 receptors on inflammatory, Efekat peptida YY(PYY) na edem šape pacova u starenju - uloga Y1 receptora na inflamatornim ćelijama",
pages = "401-400",
number = "4",
volume = "56",
url = "conv_3"
}
Stanojević, S., Vujić, V., Kovačević-Jovanović, V., Mitić, K., Kosec, D., von Hoersten, S.,& Dimitrijević, M.. (2006). Age-related effect of peptide YY(PYY) on paw edema in rat: The function of Y1 receptors on inflammatory. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(4), 400-401.
conv_3
Stanojević S, Vujić V, Kovačević-Jovanović V, Mitić K, Kosec D, von Hoersten S, Dimitrijević M. Age-related effect of peptide YY(PYY) on paw edema in rat: The function of Y1 receptors on inflammatory. in Arhiv za farmaciju. 2006;56(4):400-401.
conv_3 .
Stanojević, Stanislava, Vujić, Vesna, Kovačević-Jovanović, Vesna, Mitić, Katarina, Kosec, Duško, von Hoersten, Stephan, Dimitrijević, Mirjana, "Age-related effect of peptide YY(PYY) on paw edema in rat: The function of Y1 receptors on inflammatory" in Arhiv za farmaciju, 56, no. 4 (2006):400-401,
conv_3 .

The effect of beta-endorphin on rat paw edema involves changes in functions of inflammatory cells

Mitić, Katarina; Stanojević, Stanislava; Vujić, Vesna; Kovačević-Jovanović, Vesna; Dimitrijević, Mirjana

(Savez farmaceutskih udruženja Srbije, Beograd, 2006)

TY  - CONF
AU  - Mitić, Katarina
AU  - Stanojević, Stanislava
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Dimitrijević, Mirjana
PY  - 2006
UR  - http://intor.torlakinstitut.com/handle/123456789/223
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - The effect of beta-endorphin on rat paw edema involves changes in functions of inflammatory cells
T1  - Uticaj beta-endorfina na edem šape i funkcije inflamatornih ćelija pacova
EP  - 403
IS  - 4
SP  - 402
VL  - 56
UR  - conv_4
ER  - 
@conference{
author = "Mitić, Katarina and Stanojević, Stanislava and Vujić, Vesna and Kovačević-Jovanović, Vesna and Dimitrijević, Mirjana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "The effect of beta-endorphin on rat paw edema involves changes in functions of inflammatory cells, Uticaj beta-endorfina na edem šape i funkcije inflamatornih ćelija pacova",
pages = "403-402",
number = "4",
volume = "56",
url = "conv_4"
}
Mitić, K., Stanojević, S., Vujić, V., Kovačević-Jovanović, V.,& Dimitrijević, M.. (2006). The effect of beta-endorphin on rat paw edema involves changes in functions of inflammatory cells. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(4), 402-403.
conv_4
Mitić K, Stanojević S, Vujić V, Kovačević-Jovanović V, Dimitrijević M. The effect of beta-endorphin on rat paw edema involves changes in functions of inflammatory cells. in Arhiv za farmaciju. 2006;56(4):402-403.
conv_4 .
Mitić, Katarina, Stanojević, Stanislava, Vujić, Vesna, Kovačević-Jovanović, Vesna, Dimitrijević, Mirjana, "The effect of beta-endorphin on rat paw edema involves changes in functions of inflammatory cells" in Arhiv za farmaciju, 56, no. 4 (2006):402-403,
conv_4 .

The influence of restraint stress and beta-endorphin on inflammatory edema and macrophage function in AO rats

Mitić, Katarina; Dimitrijević, Mirjana; Vujić, Vesna; Kovačević-Jovanović, Vesna; Stanojević, Stanislava

(Elsevier, Amsterdam, 2004)

TY  - CONF
AU  - Mitić, Katarina
AU  - Dimitrijević, Mirjana
AU  - Vujić, Vesna
AU  - Kovačević-Jovanović, Vesna
AU  - Stanojević, Stanislava
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/166
PB  - Elsevier, Amsterdam
C3  - Journal of Neuroimmunology
T1  - The influence of restraint stress and beta-endorphin on inflammatory edema and macrophage function in AO rats
EP  - 140
IS  - 1-2
SP  - 140
VL  - 154
UR  - conv_158
ER  - 
@conference{
author = "Mitić, Katarina and Dimitrijević, Mirjana and Vujić, Vesna and Kovačević-Jovanović, Vesna and Stanojević, Stanislava",
year = "2004",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "The influence of restraint stress and beta-endorphin on inflammatory edema and macrophage function in AO rats",
pages = "140-140",
number = "1-2",
volume = "154",
url = "conv_158"
}
Mitić, K., Dimitrijević, M., Vujić, V., Kovačević-Jovanović, V.,& Stanojević, S.. (2004). The influence of restraint stress and beta-endorphin on inflammatory edema and macrophage function in AO rats. in Journal of Neuroimmunology
Elsevier, Amsterdam., 154(1-2), 140-140.
conv_158
Mitić K, Dimitrijević M, Vujić V, Kovačević-Jovanović V, Stanojević S. The influence of restraint stress and beta-endorphin on inflammatory edema and macrophage function in AO rats. in Journal of Neuroimmunology. 2004;154(1-2):140-140.
conv_158 .
Mitić, Katarina, Dimitrijević, Mirjana, Vujić, Vesna, Kovačević-Jovanović, Vesna, Stanojević, Stanislava, "The influence of restraint stress and beta-endorphin on inflammatory edema and macrophage function in AO rats" in Journal of Neuroimmunology, 154, no. 1-2 (2004):140-140,
conv_158 .