Minić, Rajna

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Authority KeyName Variants
orcid::0000-0003-2941-9246
  • Minić, Rajna (30)
  • Dimitrijević, Rajna (16)
Projects
Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market
Immune system plasticity during aging: Immunomodulatory capacity of oestrogens Develooment and utilization of novel and traditional technologies in production of competitive food products with added valued for national and global market - CREATING WEALTH FROM THE WEALTH OF SERBIA
Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima Molekularna genetika naslednih neurodegenerativnih i psihijatrijskih oboljenja
41021 Lallemand Health Solutions, Montreal, Canada
Charles University Project Progress Q25 Globvac program of the Research Council of Norway [196363, 234502]
Clinical-epidemiological research of diseases of public health importance in Serbia Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers
The pathogenetic mechanism in hematological malignancies Molecular determinants of innate immunity in autoimmunity and tumorogenesis
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200177 (Immunology Research Centre 'Branislav Janković' Torlak, Belgrade) Preclinical investigation of bioactive substances
Ministry of Education, Science and Technological Development of the Republic of Serbia 41021. Ministry of Education, Science and Technological Development of the Republic of Serbia, Grant 451-03-9/2021-14
Norwegian University of Life Sciences The study was supported by the Faculty of Medical Sciences, University of Kragujevac, Serbia (JP 06-12).

Author's Bibliography

Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans

Dragačević, Luka; Lopandić, Zorana; Gavrović-Jankulović, Marija; Živković, Irena; Blagojević, Veljko; Polović, Natalija; Minić, Rajna

(Springer, 2022)

TY  - JOUR
AU  - Dragačević, Luka
AU  - Lopandić, Zorana
AU  - Gavrović-Jankulović, Marija
AU  - Živković, Irena
AU  - Blagojević, Veljko
AU  - Polović, Natalija
AU  - Minić, Rajna
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/621
AB  - The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T1  - Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans
EP  - 2060
SP  - 2047
VL  - 194
DO  - 10.1007/s12010-021-03772-w
ER  - 
@article{
author = "Dragačević, Luka and Lopandić, Zorana and Gavrović-Jankulović, Marija and Živković, Irena and Blagojević, Veljko and Polović, Natalija and Minić, Rajna",
year = "2022",
abstract = "The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology",
title = "Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans",
pages = "2060-2047",
volume = "194",
doi = "10.1007/s12010-021-03772-w"
}
Dragačević, L., Lopandić, Z., Gavrović-Jankulović, M., Živković, I., Blagojević, V., Polović, N.,& Minić, R.. (2022). Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. in Applied Biochemistry and Biotechnology
Springer., 194, 2047-2060.
https://doi.org/10.1007/s12010-021-03772-w
Dragačević L, Lopandić Z, Gavrović-Jankulović M, Živković I, Blagojević V, Polović N, Minić R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. in Applied Biochemistry and Biotechnology. 2022;194:2047-2060.
doi:10.1007/s12010-021-03772-w .
Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, Minić, Rajna, "Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans" in Applied Biochemistry and Biotechnology, 194 (2022):2047-2060,
https://doi.org/10.1007/s12010-021-03772-w . .

MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation

Milovanović, Vladimir; Minić, Rajna; Vakić, J.; Ivanović, S.; Cupić, V.; Borozan, S.; Nešić, A.; Živković, Irena

(Elsevier Ltd, 2021)

TY  - JOUR
AU  - Milovanović, Vladimir
AU  - Minić, Rajna
AU  - Vakić, J.
AU  - Ivanović, S.
AU  - Cupić, V.
AU  - Borozan, S.
AU  - Nešić, A.
AU  - Živković, Irena
PY  - 2021
UR  - http://intor.torlakinstitut.com/handle/123456789/572
AB  - The MTT assay is routinely used to detect the activity of living cells. While working with Vipera ammodytes venom we detected the reduction of MTT without the presence of cells, in a concentration-dependent manner. By combining non-reducing PAGE, L-amino acid oxidase (LAAO) assays, and standard MTT assays, we established and confirmed that venom MTT reduction is catalyzed by only one enzyme, the LAAO. Even though it was previously known that the dimeric and tetrameric forms of LAAO are active, we conclude that the enzyme is also active in the monomeric form. Our results have led to the definition of a new MTT assay in a microtiter plate for in vitro testing of svLAAO activity i.e. from the venom of the V. ammodytes snake. Potentially, this method can be used for testing hemorrhagic venoms of other snakes as well as the LAAO neutralization capability of appropriate antivenoms.
PB  - Elsevier Ltd
T2  - Toxicon
T1  - MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation
EP  - 65
SP  - 57
VL  - 192
DO  - 10.1016/j.toxicon.2021.01.012
UR  - conv_586
ER  - 
@article{
author = "Milovanović, Vladimir and Minić, Rajna and Vakić, J. and Ivanović, S. and Cupić, V. and Borozan, S. and Nešić, A. and Živković, Irena",
year = "2021",
abstract = "The MTT assay is routinely used to detect the activity of living cells. While working with Vipera ammodytes venom we detected the reduction of MTT without the presence of cells, in a concentration-dependent manner. By combining non-reducing PAGE, L-amino acid oxidase (LAAO) assays, and standard MTT assays, we established and confirmed that venom MTT reduction is catalyzed by only one enzyme, the LAAO. Even though it was previously known that the dimeric and tetrameric forms of LAAO are active, we conclude that the enzyme is also active in the monomeric form. Our results have led to the definition of a new MTT assay in a microtiter plate for in vitro testing of svLAAO activity i.e. from the venom of the V. ammodytes snake. Potentially, this method can be used for testing hemorrhagic venoms of other snakes as well as the LAAO neutralization capability of appropriate antivenoms.",
publisher = "Elsevier Ltd",
journal = "Toxicon",
title = "MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation",
pages = "65-57",
volume = "192",
doi = "10.1016/j.toxicon.2021.01.012",
url = "conv_586"
}
Milovanović, V., Minić, R., Vakić, J., Ivanović, S., Cupić, V., Borozan, S., Nešić, A.,& Živković, I.. (2021). MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation. in Toxicon
Elsevier Ltd., 192, 57-65.
https://doi.org/10.1016/j.toxicon.2021.01.012
conv_586
Milovanović V, Minić R, Vakić J, Ivanović S, Cupić V, Borozan S, Nešić A, Živković I. MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation. in Toxicon. 2021;192:57-65.
doi:10.1016/j.toxicon.2021.01.012
conv_586 .
Milovanović, Vladimir, Minić, Rajna, Vakić, J., Ivanović, S., Cupić, V., Borozan, S., Nešić, A., Živković, Irena, "MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation" in Toxicon, 192 (2021):57-65,
https://doi.org/10.1016/j.toxicon.2021.01.012 .,
conv_586 .
1
1
1

Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa

Minić, Rajna; Josipović, Mirjana; Tomić-Spirić, Vesna; Gavrović-Jankulović, Marija; Perić-Popadić, Aleksandra; Prokopijević, Ivana; Ljubičić, Ana; Stamenković, Danijela; Burazer, Lidija

(MDPI, Basel, 2020)

TY  - JOUR
AU  - Minić, Rajna
AU  - Josipović, Mirjana
AU  - Tomić-Spirić, Vesna
AU  - Gavrović-Jankulović, Marija
AU  - Perić-Popadić, Aleksandra
AU  - Prokopijević, Ivana
AU  - Ljubičić, Ana
AU  - Stamenković, Danijela
AU  - Burazer, Lidija
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/565
AB  - Background and objectives: The relationship between air pollen quantity and the sensitization of allergic patients is crucial for both the diagnosis and treatment of allergic diseases. Weather conditions influence the distribution of allergenic pollen and increases in pollen concentration may negatively affect the health of allergic patients. The aim of this study was to analyze the implementation of allergen immunotherapy with regard to air pollen concentration. Material and Methods: Here we examined the relationship between Betula air pollen concentration and the usage of Betula verrucosa allergen immunotherapy in Serbia. Examination covered the period from 2015 to 2018. Measurement of airborne pollen concentration was performed with Lanzoni volumetric pollen traps. The evidence of the usage of sublingual allergen immunotherapy (SLIT) was gathered from patients with documented sensitization to specific pollen. Results: During this period tree pollens were represented with 58% +/- 21% of all measured air pollen species, while Betula pollen represented 15% +/- 8% of all tree pollens. Betula pollination peaked in April. Allergen immunotherapy to Betula verrucosa in Serbia is entirely conducted as sublingual immunotherapy and represents 47.1% +/- 1.4% of issued tree pollen SLIT. The use of pollen SLIT increased by 68% from 2015 to 2018, with an even greater increase in usage recorded for Betula SLIT-80%. Conclusions: This analysis shows a clear causative relationship between pollination and the type/prevalence of applied allergen immunotherapy. Information about the flowering seasons of allergenic plants is very important for people who suffer from allergy, for clinical allergologists, as well as for governing authorities. The presented data is of practical importance to the proper timing of immunotherapy initiation and of importance for urban landscaping. The obtained data can be the starting point for the instatement of a thorough epidemiological study and the inclusion of Serbia on the pollen map of Europe.
PB  - MDPI, Basel
T2  - Medicina-Lithuania
T1  - Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa
IS  - 2
VL  - 56
DO  - 10.3390/medicina56020059
UR  - conv_471
ER  - 
@article{
author = "Minić, Rajna and Josipović, Mirjana and Tomić-Spirić, Vesna and Gavrović-Jankulović, Marija and Perić-Popadić, Aleksandra and Prokopijević, Ivana and Ljubičić, Ana and Stamenković, Danijela and Burazer, Lidija",
year = "2020",
abstract = "Background and objectives: The relationship between air pollen quantity and the sensitization of allergic patients is crucial for both the diagnosis and treatment of allergic diseases. Weather conditions influence the distribution of allergenic pollen and increases in pollen concentration may negatively affect the health of allergic patients. The aim of this study was to analyze the implementation of allergen immunotherapy with regard to air pollen concentration. Material and Methods: Here we examined the relationship between Betula air pollen concentration and the usage of Betula verrucosa allergen immunotherapy in Serbia. Examination covered the period from 2015 to 2018. Measurement of airborne pollen concentration was performed with Lanzoni volumetric pollen traps. The evidence of the usage of sublingual allergen immunotherapy (SLIT) was gathered from patients with documented sensitization to specific pollen. Results: During this period tree pollens were represented with 58% +/- 21% of all measured air pollen species, while Betula pollen represented 15% +/- 8% of all tree pollens. Betula pollination peaked in April. Allergen immunotherapy to Betula verrucosa in Serbia is entirely conducted as sublingual immunotherapy and represents 47.1% +/- 1.4% of issued tree pollen SLIT. The use of pollen SLIT increased by 68% from 2015 to 2018, with an even greater increase in usage recorded for Betula SLIT-80%. Conclusions: This analysis shows a clear causative relationship between pollination and the type/prevalence of applied allergen immunotherapy. Information about the flowering seasons of allergenic plants is very important for people who suffer from allergy, for clinical allergologists, as well as for governing authorities. The presented data is of practical importance to the proper timing of immunotherapy initiation and of importance for urban landscaping. The obtained data can be the starting point for the instatement of a thorough epidemiological study and the inclusion of Serbia on the pollen map of Europe.",
publisher = "MDPI, Basel",
journal = "Medicina-Lithuania",
title = "Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa",
number = "2",
volume = "56",
doi = "10.3390/medicina56020059",
url = "conv_471"
}
Minić, R., Josipović, M., Tomić-Spirić, V., Gavrović-Jankulović, M., Perić-Popadić, A., Prokopijević, I., Ljubičić, A., Stamenković, D.,& Burazer, L.. (2020). Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa. in Medicina-Lithuania
MDPI, Basel., 56(2).
https://doi.org/10.3390/medicina56020059
conv_471
Minić R, Josipović M, Tomić-Spirić V, Gavrović-Jankulović M, Perić-Popadić A, Prokopijević I, Ljubičić A, Stamenković D, Burazer L. Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa. in Medicina-Lithuania. 2020;56(2).
doi:10.3390/medicina56020059
conv_471 .
Minić, Rajna, Josipović, Mirjana, Tomić-Spirić, Vesna, Gavrović-Jankulović, Marija, Perić-Popadić, Aleksandra, Prokopijević, Ivana, Ljubičić, Ana, Stamenković, Danijela, Burazer, Lidija, "Impact of Tree Pollen Distribution on Allergic Diseases in Serbia: Evidence of Implementation of Allergen Immunotherapy to Betula verrucosa" in Medicina-Lithuania, 56, no. 2 (2020),
https://doi.org/10.3390/medicina56020059 .,
conv_471 .
1
1
1

ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin

Dragačević, Luka; Đorđević, Brižita; Gavrović-Jankulović, Marija; Ilić, Vesna; Kanazir, Danijela; Minić, Rajna

(Springer, Dordrecht, 2020)

TY  - JOUR
AU  - Dragačević, Luka
AU  - Đorđević, Brižita
AU  - Gavrović-Jankulović, Marija
AU  - Ilić, Vesna
AU  - Kanazir, Danijela
AU  - Minić, Rajna
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/563
AB  - The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA(120) - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA. The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL. Among the tested lectins most selective binding was observed for RCA(120) which preferentially bound Lactobacillus casei DG. Recombinant banana lectin showed specific binding to all of the tested fungal species. The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ss-glucan rich surfaces. The binding of BL to S. boulardii could be inhibited with ss-glucan from yeast with IC50 1.81 mu g mL(-1) and to P. roqueforti with 1.10 mu g mL(-1). This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ss-glucan in solutions.
PB  - Springer, Dordrecht
T2  - Glycoconjugate Journal
T1  - ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin
EP  - 105
IS  - 1
SP  - 95
VL  - 37
DO  - 10.1007/s10719-019-09898-8
UR  - conv_468
ER  - 
@article{
author = "Dragačević, Luka and Đorđević, Brižita and Gavrović-Jankulović, Marija and Ilić, Vesna and Kanazir, Danijela and Minić, Rajna",
year = "2020",
abstract = "The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA(120) - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA. The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL. Among the tested lectins most selective binding was observed for RCA(120) which preferentially bound Lactobacillus casei DG. Recombinant banana lectin showed specific binding to all of the tested fungal species. The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ss-glucan rich surfaces. The binding of BL to S. boulardii could be inhibited with ss-glucan from yeast with IC50 1.81 mu g mL(-1) and to P. roqueforti with 1.10 mu g mL(-1). This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ss-glucan in solutions.",
publisher = "Springer, Dordrecht",
journal = "Glycoconjugate Journal",
title = "ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin",
pages = "105-95",
number = "1",
volume = "37",
doi = "10.1007/s10719-019-09898-8",
url = "conv_468"
}
Dragačević, L., Đorđević, B., Gavrović-Jankulović, M., Ilić, V., Kanazir, D.,& Minić, R.. (2020). ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin. in Glycoconjugate Journal
Springer, Dordrecht., 37(1), 95-105.
https://doi.org/10.1007/s10719-019-09898-8
conv_468
Dragačević L, Đorđević B, Gavrović-Jankulović M, Ilić V, Kanazir D, Minić R. ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin. in Glycoconjugate Journal. 2020;37(1):95-105.
doi:10.1007/s10719-019-09898-8
conv_468 .
Dragačević, Luka, Đorđević, Brižita, Gavrović-Jankulović, Marija, Ilić, Vesna, Kanazir, Danijela, Minić, Rajna, "ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin" in Glycoconjugate Journal, 37, no. 1 (2020):95-105,
https://doi.org/10.1007/s10719-019-09898-8 .,
conv_468 .
4
3
3

The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction

Mihaljević, Olgica; Živančević-Simonović, Snežana; Lučić-Tomić, Aleksandra; Živković, Irena; Minić, Rajna; Mijatović-Teodorović, Ljiljana; Jovanović, Zorica; Anđelković, Marija; Stanojević-Pirković, Marijana

(Društvo medicinskih biohemičara Srbije, Beograd i Versita, 2020)

TY  - JOUR
AU  - Mihaljević, Olgica
AU  - Živančević-Simonović, Snežana
AU  - Lučić-Tomić, Aleksandra
AU  - Živković, Irena
AU  - Minić, Rajna
AU  - Mijatović-Teodorović, Ljiljana
AU  - Jovanović, Zorica
AU  - Anđelković, Marija
AU  - Stanojević-Pirković, Marijana
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/558
AB  - Background: The aim of this study was to compare serum sclerostin concentrations in patients with thyroid dysfunction with euthyroid control subjects and to assess the relationship between sclerostin and markers of bone metabolism (osteocalcin and beta-cross-laps). Methods: The study included 30 patients with thyroid dysfunction (hypothyroidism, hyperthyroidism and subclinical hyperthyroidism) and ten euthyroid controls. Free thyroxine (FT4) was measured by radioimmunoassay, while thyroid stimulating hormone (TSH) concentration was determined immunoradiometrically. We used an ELISA kit to determine the sclerostin level. The electrochemiluminescence method was applied for measuring the bone markers. Results: Sclerostin levels were significantly lower in hypothyroid patients (p=0.009) and significantly elevated in hyperthyroid patients (p=0.008) compared to control values. Hyperthyroid patients also had higher sclerostin than patients with subclinical hyperthyroidism (p=0.013). Sclerostin concentrations were negatively correlated with TSH levels (r=-0.746, p lt 0.001), but positively with FT4 (r=0.696, p  lt  0.001). Moreover, sclerostin was positively associated with osteocalcin (r=0.605, p=0.005) and beta-cross-laps levels (r=0.573, p=0.008) in all thyroid patients. Conclusions: Serum sclerostin is significantly affected in subjects with thyroid dysfunction. Both sclerostin and thyroid status affect bone homeostasis, which is reflected through the significant correlations with osteocalcin and beta-cross-laps.
AB  - Uvod: Cilj ove studije bio je da uporedimo serumske koncentracije sklerostina kod pacijenata sa disfunkcijom štitaste žlezde u odnosu na eutiroidne kontrolne ispitanike, i da procenimo odnos između sklerostina i markera koštanog metabolizma (osteokalcina i beta-cross-lapsa) u istoj populaciji. Metode: Studijom je obuhvaćeno 30 pacijenata sa disfunkcijom štitaste žlezde (hipotireozom, hipertireozom i supkliničkom hipertireozom) i 10 eutiroidnih kontrola. Slobodni tiroksin (FT4) meren je radioimunološkom metodom, dok je koncentracija tireostimulišućeg hormona (TSH) određivana imunoradiometrijski. Za merenje nivoa sklerostina koristili smo ELISA-test. Metoda elektrohemiluminiscencije primenjena je za merenje koncentracije koštanih markera. Rezultati: Nivo sklerostina bio je značajno niži kod bolesnika sa hipotireozom (p = 0,009) odnosno značajno viši kod bolesnika sa hipertireozom (p = 0,008) u poređenju sa vrednostima kod eutiroidnih kontrolnih ispitanika. Pacijenti sa hipertireozom su takođe imali statistički značajno viši nivo sklerostina u odnosu na bolesnike sa supkliničkom hipertireozom (p = 0,013). Pokazana je negativna korelacija koncentracije sklerostina i TSH (r = -0,746, p  lt  0,001), odnosno pozitivna korelacija sa FT4 (r = 0,696, p  lt  0,001) kod pacijenata sa tireoidnom disfunkcijom. Štaviše, sklerostin pozitivno korelira sa koncentracijom osteokalcina (r = 0,605, p = 0,005) i beta-cross-lapsa (r = 0,573, p = 0,008) kod ovih pacijenata. Zaključak: Serumski nivoi sklerostina izmenjeni su kod osoba sa disfunkcijom štitaste žlezde. Sklerostin zajedno sa tireoidnim statusom utiče na koštani metabolizam, što se ogleda u njegovoj značajnoj korelaciji sa osteokalcinom i beta-cross-lapsom.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction
T1  - Veza cirkulišućeg nivoa sklerostina sa markerima metabolizma kostiju kod pacijenata sa poremećajem rada štitaste žlezde
EP  - 443
IS  - 4
SP  - 436
VL  - 39
DO  - 10.5937/jomb0-24943
UR  - conv_72
ER  - 
@article{
author = "Mihaljević, Olgica and Živančević-Simonović, Snežana and Lučić-Tomić, Aleksandra and Živković, Irena and Minić, Rajna and Mijatović-Teodorović, Ljiljana and Jovanović, Zorica and Anđelković, Marija and Stanojević-Pirković, Marijana",
year = "2020",
abstract = "Background: The aim of this study was to compare serum sclerostin concentrations in patients with thyroid dysfunction with euthyroid control subjects and to assess the relationship between sclerostin and markers of bone metabolism (osteocalcin and beta-cross-laps). Methods: The study included 30 patients with thyroid dysfunction (hypothyroidism, hyperthyroidism and subclinical hyperthyroidism) and ten euthyroid controls. Free thyroxine (FT4) was measured by radioimmunoassay, while thyroid stimulating hormone (TSH) concentration was determined immunoradiometrically. We used an ELISA kit to determine the sclerostin level. The electrochemiluminescence method was applied for measuring the bone markers. Results: Sclerostin levels were significantly lower in hypothyroid patients (p=0.009) and significantly elevated in hyperthyroid patients (p=0.008) compared to control values. Hyperthyroid patients also had higher sclerostin than patients with subclinical hyperthyroidism (p=0.013). Sclerostin concentrations were negatively correlated with TSH levels (r=-0.746, p lt 0.001), but positively with FT4 (r=0.696, p  lt  0.001). Moreover, sclerostin was positively associated with osteocalcin (r=0.605, p=0.005) and beta-cross-laps levels (r=0.573, p=0.008) in all thyroid patients. Conclusions: Serum sclerostin is significantly affected in subjects with thyroid dysfunction. Both sclerostin and thyroid status affect bone homeostasis, which is reflected through the significant correlations with osteocalcin and beta-cross-laps., Uvod: Cilj ove studije bio je da uporedimo serumske koncentracije sklerostina kod pacijenata sa disfunkcijom štitaste žlezde u odnosu na eutiroidne kontrolne ispitanike, i da procenimo odnos između sklerostina i markera koštanog metabolizma (osteokalcina i beta-cross-lapsa) u istoj populaciji. Metode: Studijom je obuhvaćeno 30 pacijenata sa disfunkcijom štitaste žlezde (hipotireozom, hipertireozom i supkliničkom hipertireozom) i 10 eutiroidnih kontrola. Slobodni tiroksin (FT4) meren je radioimunološkom metodom, dok je koncentracija tireostimulišućeg hormona (TSH) određivana imunoradiometrijski. Za merenje nivoa sklerostina koristili smo ELISA-test. Metoda elektrohemiluminiscencije primenjena je za merenje koncentracije koštanih markera. Rezultati: Nivo sklerostina bio je značajno niži kod bolesnika sa hipotireozom (p = 0,009) odnosno značajno viši kod bolesnika sa hipertireozom (p = 0,008) u poređenju sa vrednostima kod eutiroidnih kontrolnih ispitanika. Pacijenti sa hipertireozom su takođe imali statistički značajno viši nivo sklerostina u odnosu na bolesnike sa supkliničkom hipertireozom (p = 0,013). Pokazana je negativna korelacija koncentracije sklerostina i TSH (r = -0,746, p  lt  0,001), odnosno pozitivna korelacija sa FT4 (r = 0,696, p  lt  0,001) kod pacijenata sa tireoidnom disfunkcijom. Štaviše, sklerostin pozitivno korelira sa koncentracijom osteokalcina (r = 0,605, p = 0,005) i beta-cross-lapsa (r = 0,573, p = 0,008) kod ovih pacijenata. Zaključak: Serumski nivoi sklerostina izmenjeni su kod osoba sa disfunkcijom štitaste žlezde. Sklerostin zajedno sa tireoidnim statusom utiče na koštani metabolizam, što se ogleda u njegovoj značajnoj korelaciji sa osteokalcinom i beta-cross-lapsom.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction, Veza cirkulišućeg nivoa sklerostina sa markerima metabolizma kostiju kod pacijenata sa poremećajem rada štitaste žlezde",
pages = "443-436",
number = "4",
volume = "39",
doi = "10.5937/jomb0-24943",
url = "conv_72"
}
Mihaljević, O., Živančević-Simonović, S., Lučić-Tomić, A., Živković, I., Minić, R., Mijatović-Teodorović, L., Jovanović, Z., Anđelković, M.,& Stanojević-Pirković, M.. (2020). The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 39(4), 436-443.
https://doi.org/10.5937/jomb0-24943
conv_72
Mihaljević O, Živančević-Simonović S, Lučić-Tomić A, Živković I, Minić R, Mijatović-Teodorović L, Jovanović Z, Anđelković M, Stanojević-Pirković M. The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction. in Journal of Medical Biochemistry. 2020;39(4):436-443.
doi:10.5937/jomb0-24943
conv_72 .
Mihaljević, Olgica, Živančević-Simonović, Snežana, Lučić-Tomić, Aleksandra, Živković, Irena, Minić, Rajna, Mijatović-Teodorović, Ljiljana, Jovanović, Zorica, Anđelković, Marija, Stanojević-Pirković, Marijana, "The association of circulating sclerostin level with markers of bone metabolism in patients with thyroid dysfunction" in Journal of Medical Biochemistry, 39, no. 4 (2020):436-443,
https://doi.org/10.5937/jomb0-24943 .,
conv_72 .
1
2

Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter

Michalickova, Danica; Minić, Rajna; Kotur-Stevuljević, Jelena; Anđelković, Marija; Dikić, Nenad; Kostić-Vučićević, Marija; Slanar, Ondrej; Đorđević, Brižita

(Lippincott Williams & Wilkins, Philadelphia, 2020)

TY  - JOUR
AU  - Michalickova, Danica
AU  - Minić, Rajna
AU  - Kotur-Stevuljević, Jelena
AU  - Anđelković, Marija
AU  - Dikić, Nenad
AU  - Kostić-Vučićević, Marija
AU  - Slanar, Ondrej
AU  - Đorđević, Brižita
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/545
AB  - Michalickova, D, Minic, R, Kotur-Stevuljevic, J, Andjelkovic, M, Dikic, N, Kostic-Vucicevic, M, Slanar, O, and Djordjevic, B. Changes in parameters of oxidative stress, immunity, and behavior in endurance athletes during a preparation period in winter.J Strength Cond Res34(10): 2965-2973, 2020-The current study monitored markers of immunological and oxidative status in 9 male elite endurance athletes: V?o(2)max: 68 +/- 11 ml center dot kg(-1)center dot min(-1), age: 24 +/- 2.5 years, and training loads: 128 +/- 21 metabolic equivalents-h center dot wk(-1)during a 3-month preparation period in winter (January-March). Self-rated state of moods evaluation (by Profile of Mood States questionnaire) was performed, and blood samples were collected at the beginning and end of the study. Spectrophotometric methods and enzyme-linked immunosorbent assay were used for parameters' determination. The level of concanavalin A (ConA)-stimulated interferon-gamma (IFN-gamma) from peripheral blood mononuclear cells (PBMCs) was increased (562 [147-852] vs. 1,097 [451-1842] pg center dot ml(-1),p= 0.013). Also, the level of transforming growth factor-1 (TGF-beta 1) in serum was elevated (2.5 [1.4-5.1] vs. 7.2 [4.9-8.2] ng center dot ml(-1),p= 0.015). There was no change in the level of peptidoglycan (PGN)-stimulated interleukin (IL)-10 from PBMCs. There were no significant changes in PBMCs proliferation/viability on stimulation with ConA and PGN during the study. No changes in superoxide dismutase, prooxidative-antioxidative balance, total oxidant status (TOS), and thiobarbituric acid reactive substances were observed along the study. Total antioxidant status (TAS) was increased (910 +/- 174 vs. 1,090 +/- 102 mu mol center dot L-1,p= 0.018), and activity of paraoxonase (PON1) was decreased (523 +/- 295 vs. 335 +/- 183 U center dot L-1,p= 0.003) at the end of the study. Advanced oxidation protein products were increased (25 +/- 7.9 vs. 42 +/- 7.6 mu mol center dot L-1,p= 0.011). The self-rated sense of vigor significantly declined (20 +/- 2.1 vs. 14 +/- 3.4,p= 0.045). In conclusion, 3 months of regular training in winter induced prominent changes in cytokines, biomarkers of oxidative stress, and antioxidative enzyme activity. These changes might increase susceptibility of athletes to disease and muscle damage and consequently lead to performance reduction.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Strength and Conditioning Research
T1  - Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter
EP  - 2973
IS  - 10
SP  - 2965
VL  - 34
DO  - 10.1519/JSC.0000000000002780
UR  - conv_483
ER  - 
@article{
author = "Michalickova, Danica and Minić, Rajna and Kotur-Stevuljević, Jelena and Anđelković, Marija and Dikić, Nenad and Kostić-Vučićević, Marija and Slanar, Ondrej and Đorđević, Brižita",
year = "2020",
abstract = "Michalickova, D, Minic, R, Kotur-Stevuljevic, J, Andjelkovic, M, Dikic, N, Kostic-Vucicevic, M, Slanar, O, and Djordjevic, B. Changes in parameters of oxidative stress, immunity, and behavior in endurance athletes during a preparation period in winter.J Strength Cond Res34(10): 2965-2973, 2020-The current study monitored markers of immunological and oxidative status in 9 male elite endurance athletes: V?o(2)max: 68 +/- 11 ml center dot kg(-1)center dot min(-1), age: 24 +/- 2.5 years, and training loads: 128 +/- 21 metabolic equivalents-h center dot wk(-1)during a 3-month preparation period in winter (January-March). Self-rated state of moods evaluation (by Profile of Mood States questionnaire) was performed, and blood samples were collected at the beginning and end of the study. Spectrophotometric methods and enzyme-linked immunosorbent assay were used for parameters' determination. The level of concanavalin A (ConA)-stimulated interferon-gamma (IFN-gamma) from peripheral blood mononuclear cells (PBMCs) was increased (562 [147-852] vs. 1,097 [451-1842] pg center dot ml(-1),p= 0.013). Also, the level of transforming growth factor-1 (TGF-beta 1) in serum was elevated (2.5 [1.4-5.1] vs. 7.2 [4.9-8.2] ng center dot ml(-1),p= 0.015). There was no change in the level of peptidoglycan (PGN)-stimulated interleukin (IL)-10 from PBMCs. There were no significant changes in PBMCs proliferation/viability on stimulation with ConA and PGN during the study. No changes in superoxide dismutase, prooxidative-antioxidative balance, total oxidant status (TOS), and thiobarbituric acid reactive substances were observed along the study. Total antioxidant status (TAS) was increased (910 +/- 174 vs. 1,090 +/- 102 mu mol center dot L-1,p= 0.018), and activity of paraoxonase (PON1) was decreased (523 +/- 295 vs. 335 +/- 183 U center dot L-1,p= 0.003) at the end of the study. Advanced oxidation protein products were increased (25 +/- 7.9 vs. 42 +/- 7.6 mu mol center dot L-1,p= 0.011). The self-rated sense of vigor significantly declined (20 +/- 2.1 vs. 14 +/- 3.4,p= 0.045). In conclusion, 3 months of regular training in winter induced prominent changes in cytokines, biomarkers of oxidative stress, and antioxidative enzyme activity. These changes might increase susceptibility of athletes to disease and muscle damage and consequently lead to performance reduction.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Strength and Conditioning Research",
title = "Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter",
pages = "2973-2965",
number = "10",
volume = "34",
doi = "10.1519/JSC.0000000000002780",
url = "conv_483"
}
Michalickova, D., Minić, R., Kotur-Stevuljević, J., Anđelković, M., Dikić, N., Kostić-Vučićević, M., Slanar, O.,& Đorđević, B.. (2020). Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter. in Journal of Strength and Conditioning Research
Lippincott Williams & Wilkins, Philadelphia., 34(10), 2965-2973.
https://doi.org/10.1519/JSC.0000000000002780
conv_483
Michalickova D, Minić R, Kotur-Stevuljević J, Anđelković M, Dikić N, Kostić-Vučićević M, Slanar O, Đorđević B. Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter. in Journal of Strength and Conditioning Research. 2020;34(10):2965-2973.
doi:10.1519/JSC.0000000000002780
conv_483 .
Michalickova, Danica, Minić, Rajna, Kotur-Stevuljević, Jelena, Anđelković, Marija, Dikić, Nenad, Kostić-Vučićević, Marija, Slanar, Ondrej, Đorđević, Brižita, "Changes in Parameters of Oxidative Stress, Immunity, and Behavior in Endurance Athletes During a Preparation Period in Winter" in Journal of Strength and Conditioning Research, 34, no. 10 (2020):2965-2973,
https://doi.org/10.1519/JSC.0000000000002780 .,
conv_483 .
5
4
4

Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults

Dragačević, Luka; Minić, Rajna; Michalickova, Danica; Ilić, Vesna; Đorđević, Brižita

(Lippincott Williams & Wilkins, Philadelphia, 2020)

TY  - CONF
AU  - Dragačević, Luka
AU  - Minić, Rajna
AU  - Michalickova, Danica
AU  - Ilić, Vesna
AU  - Đorđević, Brižita
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/568
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Clinical Gastroenterology
T1  - Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults
EP  - S18
SP  - S18
VL  - 54
UR  - conv_474
ER  - 
@conference{
author = "Dragačević, Luka and Minić, Rajna and Michalickova, Danica and Ilić, Vesna and Đorđević, Brižita",
year = "2020",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Clinical Gastroenterology",
title = "Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults",
pages = "S18-S18",
volume = "54",
url = "conv_474"
}
Dragačević, L., Minić, R., Michalickova, D., Ilić, V.,& Đorđević, B.. (2020). Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults. in Journal of Clinical Gastroenterology
Lippincott Williams & Wilkins, Philadelphia., 54, S18-S18.
conv_474
Dragačević L, Minić R, Michalickova D, Ilić V, Đorđević B. Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults. in Journal of Clinical Gastroenterology. 2020;54:S18-S18.
conv_474 .
Dragačević, Luka, Minić, Rajna, Michalickova, Danica, Ilić, Vesna, Đorđević, Brižita, "Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults" in Journal of Clinical Gastroenterology, 54 (2020):S18-S18,
conv_474 .

Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa

Dragačević, Luka; Đorđević, Brižita; Gavrović-Jankulović, Marija; Ilić, Vesna; Minić, Rajna

(Lippincott Williams & Wilkins, Philadelphia, 2020)

TY  - CONF
AU  - Dragačević, Luka
AU  - Đorđević, Brižita
AU  - Gavrović-Jankulović, Marija
AU  - Ilić, Vesna
AU  - Minić, Rajna
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/567
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Clinical Gastroenterology
T1  - Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa
EP  - S13
SP  - S13
VL  - 54
UR  - conv_473
ER  - 
@conference{
author = "Dragačević, Luka and Đorđević, Brižita and Gavrović-Jankulović, Marija and Ilić, Vesna and Minić, Rajna",
year = "2020",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Clinical Gastroenterology",
title = "Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa",
pages = "S13-S13",
volume = "54",
url = "conv_473"
}
Dragačević, L., Đorđević, B., Gavrović-Jankulović, M., Ilić, V.,& Minić, R.. (2020). Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa. in Journal of Clinical Gastroenterology
Lippincott Williams & Wilkins, Philadelphia., 54, S13-S13.
conv_473
Dragačević L, Đorđević B, Gavrović-Jankulović M, Ilić V, Minić R. Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa. in Journal of Clinical Gastroenterology. 2020;54:S13-S13.
conv_473 .
Dragačević, Luka, Đorđević, Brižita, Gavrović-Jankulović, Marija, Ilić, Vesna, Minić, Rajna, "Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa" in Journal of Clinical Gastroenterology, 54 (2020):S13-S13,
conv_473 .

Antimicrobial immunoglobulin prophylaxis and therapy

Živković, Irena; Minić, Rajna; Dimitrijević, Ljiljana

(Bentham Science Publishers, 2020)

TY  - CHAP
AU  - Živković, Irena
AU  - Minić, Rajna
AU  - Dimitrijević, Ljiljana
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/549
AB  - Immunoglobulins, either natural or induced, represent powerful means for eliminating microbial threats. For decades, vaccines have been used which function by inducing antibodies for the successful elimination of microbes, preventing disease and mortality. Polyclonal immunoglobulin products from human plasma were used for the first time to treat immune deficiency during the 1950s. Nowadays, the administration of immunoglobulin, either intravenous or subcutaneous, is the cornerstone of the treatment of primary immunodeficiency affecting the humoral immune system. The therapy can help people with weakened or underdeveloped immune system fight off infections. This is also the treatment of choice for certain autoimmune and neurological diseases. The polyclonal antibody preparation consists of both natural antibodies and antigen-induced antibodies and reflects the history of antigen experience of the donor population. Natural antibodies are germline-encoded, polyspecific, low-affinity antibodies that also have a regulatory role in maintaining the immune system homeostasis. Antibody preparations, enriched for certain desired specificities, or hyperimmune human globulins are used for prophylaxis and treatment of infectious diseases with a high mortality rate. It was shown that lymphocytes from survivors of viral infections, without available vaccines, could be used for protective monoclonal antibodies production. This chapter provides insight into the current knowledge of the usage of antibody preparations, efficacy and mechanism of action, with respect to specific diseases, including the treatment of infectious diseases and future prospects.
PB  - Bentham Science Publishers
T2  - Frontiers in Clinical Drug Research - Anti Infectives
T1  - Antimicrobial immunoglobulin prophylaxis and therapy
EP  - 205
IS  - 1
SP  - 165
VL  - 6
DO  - 10.2174/9789811425745120060007
UR  - conv_588
ER  - 
@inbook{
author = "Živković, Irena and Minić, Rajna and Dimitrijević, Ljiljana",
year = "2020",
abstract = "Immunoglobulins, either natural or induced, represent powerful means for eliminating microbial threats. For decades, vaccines have been used which function by inducing antibodies for the successful elimination of microbes, preventing disease and mortality. Polyclonal immunoglobulin products from human plasma were used for the first time to treat immune deficiency during the 1950s. Nowadays, the administration of immunoglobulin, either intravenous or subcutaneous, is the cornerstone of the treatment of primary immunodeficiency affecting the humoral immune system. The therapy can help people with weakened or underdeveloped immune system fight off infections. This is also the treatment of choice for certain autoimmune and neurological diseases. The polyclonal antibody preparation consists of both natural antibodies and antigen-induced antibodies and reflects the history of antigen experience of the donor population. Natural antibodies are germline-encoded, polyspecific, low-affinity antibodies that also have a regulatory role in maintaining the immune system homeostasis. Antibody preparations, enriched for certain desired specificities, or hyperimmune human globulins are used for prophylaxis and treatment of infectious diseases with a high mortality rate. It was shown that lymphocytes from survivors of viral infections, without available vaccines, could be used for protective monoclonal antibodies production. This chapter provides insight into the current knowledge of the usage of antibody preparations, efficacy and mechanism of action, with respect to specific diseases, including the treatment of infectious diseases and future prospects.",
publisher = "Bentham Science Publishers",
journal = "Frontiers in Clinical Drug Research - Anti Infectives",
booktitle = "Antimicrobial immunoglobulin prophylaxis and therapy",
pages = "205-165",
number = "1",
volume = "6",
doi = "10.2174/9789811425745120060007",
url = "conv_588"
}
Živković, I., Minić, R.,& Dimitrijević, L.. (2020). Antimicrobial immunoglobulin prophylaxis and therapy. in Frontiers in Clinical Drug Research - Anti Infectives
Bentham Science Publishers., 6(1), 165-205.
https://doi.org/10.2174/9789811425745120060007
conv_588
Živković I, Minić R, Dimitrijević L. Antimicrobial immunoglobulin prophylaxis and therapy. in Frontiers in Clinical Drug Research - Anti Infectives. 2020;6(1):165-205.
doi:10.2174/9789811425745120060007
conv_588 .
Živković, Irena, Minić, Rajna, Dimitrijević, Ljiljana, "Antimicrobial immunoglobulin prophylaxis and therapy" in Frontiers in Clinical Drug Research - Anti Infectives, 6, no. 1 (2020):165-205,
https://doi.org/10.2174/9789811425745120060007 .,
conv_588 .
1

Optimization, Validation and Standardization of ELISA

Živković, Irena; Minić, Rajna

(IntechOpen, 2020)

TY  - CHAP
AU  - Živković, Irena
AU  - Minić, Rajna
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/548
PB  - IntechOpen
T2  - ELISA Test-Perspectives and Applications
T1  - Optimization, Validation and Standardization of ELISA
DO  - 10.5772/intechopen.94338
UR  - conv_661
ER  - 
@inbook{
author = "Živković, Irena and Minić, Rajna",
year = "2020",
publisher = "IntechOpen",
journal = "ELISA Test-Perspectives and Applications",
booktitle = "Optimization, Validation and Standardization of ELISA",
doi = "10.5772/intechopen.94338",
url = "conv_661"
}
Živković, I.,& Minić, R.. (2020). Optimization, Validation and Standardization of ELISA. in ELISA Test-Perspectives and Applications
IntechOpen..
https://doi.org/10.5772/intechopen.94338
conv_661
Živković I, Minić R. Optimization, Validation and Standardization of ELISA. in ELISA Test-Perspectives and Applications. 2020;.
doi:10.5772/intechopen.94338
conv_661 .
Živković, Irena, Minić, Rajna, "Optimization, Validation and Standardization of ELISA" in ELISA Test-Perspectives and Applications (2020),
https://doi.org/10.5772/intechopen.94338 .,
conv_661 .
5

Profiling of microorganism-binding serum antibody specificities in professional athletes

Minić, Rajna; Papić, Zlatko; Đorđević, Brižita; Michalickova, Danica; Ilić, Vesna; Mathiesen, Geir; Živković, Irena; Pantić, Visnja; Dimitrijević, Ljiljana

(Public Library Science, San Francisco, 2018)

TY  - JOUR
AU  - Minić, Rajna
AU  - Papić, Zlatko
AU  - Đorđević, Brižita
AU  - Michalickova, Danica
AU  - Ilić, Vesna
AU  - Mathiesen, Geir
AU  - Živković, Irena
AU  - Pantić, Visnja
AU  - Dimitrijević, Ljiljana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/508
AB  - The goal of this work was to elucidate similarities between microorganisms from the perspective of the humoral immune system reactivity in professional athletes. The reactivity of serum IgG of 14 young, individuals was analyzed to 23 selected microorganisms as antigens by use of the in house ELISA. Serum IgM and IgA reactivity was also analyzed and a control group of sex and age matched individuals was used for comparison. The obtained absorbance levels were used as a string of values to correlate the reactivity to different microorganisms. IgM was found to be the most cross reactive antibody class, Pearson's r = 0.7 +/- 0.92, for very distant bacterial species such as Lactobacillus and E. coli. High correlation in IgG levels was found for Gammaproteobacteria and LPS (from E. coli) (r = 0.77 for LPS vs. P. aeruginosa to r = 0.98 for LPS vs. E. coli), whereas this correlation was lower in the control group (r = 0.49 for LPS vs. P. aeruginosa to r = 0.66 for LPS vs. E. coli). The correlation was also analyzed between total IgG and IgG subclasses specific for the same microorganism, and IgG2 was identified as the main subclass recognising different microorganisms, as well as recognising LPS. Upon correlation of IgG with IgA for the same microorganism absence of or negative correlation was found between bacteria-specific IgA and IgG in case of Lactobacillus and Staphylococcusgeni, whereas correlation was absent or positive for Candida albicans, Enterococcusfaecalis, Streptococcus species tested in professional athletes. Opposite results were obtained for the control group. Outlined here is a simple experimental procedure and data analysis which yields functional significance and which can be used for determining the similarities between microorganisms from the aspect of the humoral immune system, for determining the main IgG subclass involved in an immune response as well as for the analysis of different target populations.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Profiling of microorganism-binding serum antibody specificities in professional athletes
IS  - 9
VL  - 13
DO  - 10.1371/journal.pone.0203665
UR  - conv_441
ER  - 
@article{
author = "Minić, Rajna and Papić, Zlatko and Đorđević, Brižita and Michalickova, Danica and Ilić, Vesna and Mathiesen, Geir and Živković, Irena and Pantić, Visnja and Dimitrijević, Ljiljana",
year = "2018",
abstract = "The goal of this work was to elucidate similarities between microorganisms from the perspective of the humoral immune system reactivity in professional athletes. The reactivity of serum IgG of 14 young, individuals was analyzed to 23 selected microorganisms as antigens by use of the in house ELISA. Serum IgM and IgA reactivity was also analyzed and a control group of sex and age matched individuals was used for comparison. The obtained absorbance levels were used as a string of values to correlate the reactivity to different microorganisms. IgM was found to be the most cross reactive antibody class, Pearson's r = 0.7 +/- 0.92, for very distant bacterial species such as Lactobacillus and E. coli. High correlation in IgG levels was found for Gammaproteobacteria and LPS (from E. coli) (r = 0.77 for LPS vs. P. aeruginosa to r = 0.98 for LPS vs. E. coli), whereas this correlation was lower in the control group (r = 0.49 for LPS vs. P. aeruginosa to r = 0.66 for LPS vs. E. coli). The correlation was also analyzed between total IgG and IgG subclasses specific for the same microorganism, and IgG2 was identified as the main subclass recognising different microorganisms, as well as recognising LPS. Upon correlation of IgG with IgA for the same microorganism absence of or negative correlation was found between bacteria-specific IgA and IgG in case of Lactobacillus and Staphylococcusgeni, whereas correlation was absent or positive for Candida albicans, Enterococcusfaecalis, Streptococcus species tested in professional athletes. Opposite results were obtained for the control group. Outlined here is a simple experimental procedure and data analysis which yields functional significance and which can be used for determining the similarities between microorganisms from the aspect of the humoral immune system, for determining the main IgG subclass involved in an immune response as well as for the analysis of different target populations.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Profiling of microorganism-binding serum antibody specificities in professional athletes",
number = "9",
volume = "13",
doi = "10.1371/journal.pone.0203665",
url = "conv_441"
}
Minić, R., Papić, Z., Đorđević, B., Michalickova, D., Ilić, V., Mathiesen, G., Živković, I., Pantić, V.,& Dimitrijević, L.. (2018). Profiling of microorganism-binding serum antibody specificities in professional athletes. in PLoS One
Public Library Science, San Francisco., 13(9).
https://doi.org/10.1371/journal.pone.0203665
conv_441
Minić R, Papić Z, Đorđević B, Michalickova D, Ilić V, Mathiesen G, Živković I, Pantić V, Dimitrijević L. Profiling of microorganism-binding serum antibody specificities in professional athletes. in PLoS One. 2018;13(9).
doi:10.1371/journal.pone.0203665
conv_441 .
Minić, Rajna, Papić, Zlatko, Đorđević, Brižita, Michalickova, Danica, Ilić, Vesna, Mathiesen, Geir, Živković, Irena, Pantić, Visnja, Dimitrijević, Ljiljana, "Profiling of microorganism-binding serum antibody specificities in professional athletes" in PLoS One, 13, no. 9 (2018),
https://doi.org/10.1371/journal.pone.0203665 .,
conv_441 .
9
5
4
5

Selected Microorganisms from an Antibody Binding Perspective in Professional Athletes

Minić, Rajna; Papić, Zlatko; Đorđević, Brižita; Michalickova, Danica; Ilić, Vesna; Mathiesen, Geir; Živković, Irena; Dimitrijević, Ljiljana

(Lippincott Williams & Wilkins, Philadelphia, 2018)

TY  - CONF
AU  - Minić, Rajna
AU  - Papić, Zlatko
AU  - Đorđević, Brižita
AU  - Michalickova, Danica
AU  - Ilić, Vesna
AU  - Mathiesen, Geir
AU  - Živković, Irena
AU  - Dimitrijević, Ljiljana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/507
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Clinical Gastroenterology
T1  - Selected Microorganisms from an Antibody Binding Perspective in Professional Athletes
EP  - S99
SP  - S99
VL  - 52
UR  - conv_445
ER  - 
@conference{
author = "Minić, Rajna and Papić, Zlatko and Đorđević, Brižita and Michalickova, Danica and Ilić, Vesna and Mathiesen, Geir and Živković, Irena and Dimitrijević, Ljiljana",
year = "2018",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Clinical Gastroenterology",
title = "Selected Microorganisms from an Antibody Binding Perspective in Professional Athletes",
pages = "S99-S99",
volume = "52",
url = "conv_445"
}
Minić, R., Papić, Z., Đorđević, B., Michalickova, D., Ilić, V., Mathiesen, G., Živković, I.,& Dimitrijević, L.. (2018). Selected Microorganisms from an Antibody Binding Perspective in Professional Athletes. in Journal of Clinical Gastroenterology
Lippincott Williams & Wilkins, Philadelphia., 52, S99-S99.
conv_445
Minić R, Papić Z, Đorđević B, Michalickova D, Ilić V, Mathiesen G, Živković I, Dimitrijević L. Selected Microorganisms from an Antibody Binding Perspective in Professional Athletes. in Journal of Clinical Gastroenterology. 2018;52:S99-S99.
conv_445 .
Minić, Rajna, Papić, Zlatko, Đorđević, Brižita, Michalickova, Danica, Ilić, Vesna, Mathiesen, Geir, Živković, Irena, Dimitrijević, Ljiljana, "Selected Microorganisms from an Antibody Binding Perspective in Professional Athletes" in Journal of Clinical Gastroenterology, 52 (2018):S99-S99,
conv_445 .

Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type

Živković, Irena; Petrović, Raisa; Arsenović-Ranin, Nevena; Petrušić, Vladimir; Minić, Rajna; Bufan, Biljana; Popović, Olga; Leposavić, Gordana

(Academic Press Ltd- Elsevier Science Ltd, London, 2018)

TY  - JOUR
AU  - Živković, Irena
AU  - Petrović, Raisa
AU  - Arsenović-Ranin, Nevena
AU  - Petrušić, Vladimir
AU  - Minić, Rajna
AU  - Bufan, Biljana
AU  - Popović, Olga
AU  - Leposavić, Gordana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/522
AB  - The study explored influence of biological sex on development of humoral immune response to seasonal trivalent whole inactivated virus (WIV) and split virus (SV) influenza vaccines in outbred Swiss mouse model. To this end, mice of both sexes were immunized with WIV (WIV mice) and SV vaccines (SV mice) and examined for specific antibody response. Irrespective of sex, total IgG and neutralizing antibody responses to distinct virus strains were weaker in SV than in WIV mice. In WIV mice of both sexes, irrespective of strain specificity, IgG isotype response was dominated by IgG2a antibodies, while in SV mice nearly equal representation of IgG2a and IgG1 antibodies was found. The analyses of sex differences showed higher titers of H1N1-specific and both H1N1- and H3N2-specific total IgG and neutralizing antibodies in female WIV and SV mice, respectively. Additionally, sexual dimorphism in IgG subclass profile depended on vaccine type. Specifically, compared with males, in females WIV shifted IgG2a/IgG1 antibody ratio towards IgG2a isotype on the account of weaker IgG1 response, whereas in SV mice, irrespective of virus strain, IgG2a and IgG1 isotypes were equally represented in both sexes. These findings indicate the vaccine type-dependent sex bias in antibody response to inactivated influenza vaccines.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Biologicals
T1  - Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type
EP  - 24
SP  - 18
VL  - 52
DO  - 10.1016/j.biologicals.2018.01.007
UR  - conv_431
ER  - 
@article{
author = "Živković, Irena and Petrović, Raisa and Arsenović-Ranin, Nevena and Petrušić, Vladimir and Minić, Rajna and Bufan, Biljana and Popović, Olga and Leposavić, Gordana",
year = "2018",
abstract = "The study explored influence of biological sex on development of humoral immune response to seasonal trivalent whole inactivated virus (WIV) and split virus (SV) influenza vaccines in outbred Swiss mouse model. To this end, mice of both sexes were immunized with WIV (WIV mice) and SV vaccines (SV mice) and examined for specific antibody response. Irrespective of sex, total IgG and neutralizing antibody responses to distinct virus strains were weaker in SV than in WIV mice. In WIV mice of both sexes, irrespective of strain specificity, IgG isotype response was dominated by IgG2a antibodies, while in SV mice nearly equal representation of IgG2a and IgG1 antibodies was found. The analyses of sex differences showed higher titers of H1N1-specific and both H1N1- and H3N2-specific total IgG and neutralizing antibodies in female WIV and SV mice, respectively. Additionally, sexual dimorphism in IgG subclass profile depended on vaccine type. Specifically, compared with males, in females WIV shifted IgG2a/IgG1 antibody ratio towards IgG2a isotype on the account of weaker IgG1 response, whereas in SV mice, irrespective of virus strain, IgG2a and IgG1 isotypes were equally represented in both sexes. These findings indicate the vaccine type-dependent sex bias in antibody response to inactivated influenza vaccines.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Biologicals",
title = "Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type",
pages = "24-18",
volume = "52",
doi = "10.1016/j.biologicals.2018.01.007",
url = "conv_431"
}
Živković, I., Petrović, R., Arsenović-Ranin, N., Petrušić, V., Minić, R., Bufan, B., Popović, O.,& Leposavić, G.. (2018). Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type. in Biologicals
Academic Press Ltd- Elsevier Science Ltd, London., 52, 18-24.
https://doi.org/10.1016/j.biologicals.2018.01.007
conv_431
Živković I, Petrović R, Arsenović-Ranin N, Petrušić V, Minić R, Bufan B, Popović O, Leposavić G. Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type. in Biologicals. 2018;52:18-24.
doi:10.1016/j.biologicals.2018.01.007
conv_431 .
Živković, Irena, Petrović, Raisa, Arsenović-Ranin, Nevena, Petrušić, Vladimir, Minić, Rajna, Bufan, Biljana, Popović, Olga, Leposavić, Gordana, "Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type" in Biologicals, 52 (2018):18-24,
https://doi.org/10.1016/j.biologicals.2018.01.007 .,
conv_431 .
18
15
18

Mouse strain and sex as determinants of immune response to trivalent influenza vaccine

Petrović, Raisa; Bufan, Biljana; Arsenović-Ranin, Nevena; Živković, Irena; Minić, Rajna; Radojević, Katarina; Leposavić, Gordana

(Pergamon-Elsevier Science Ltd, Oxford, 2018)

TY  - JOUR
AU  - Petrović, Raisa
AU  - Bufan, Biljana
AU  - Arsenović-Ranin, Nevena
AU  - Živković, Irena
AU  - Minić, Rajna
AU  - Radojević, Katarina
AU  - Leposavić, Gordana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/501
AB  - Aims: The study examined the influence of sex and mouse strain on germinal center (GC) reaction and antibody responses to seasonal split trivalent influenza vaccine (TIV). Main methods: C57BL/6 and BALB/c mice of both sexes were immunized with TIV and examined for specific antibody response by ELISA. Splenic T follicular regulatory (Tfr), T follicular helper (Tfh) and GC B cells are detected by flow cytometry. The proliferative response of splenocytes, and concentrations of IFN-gamma and IL-4 upon restimulation with vaccine antigens were examined by 7-AAD staining and ELISA, respectively. Key findings: BALB/c mice developed more robust IgG responses to vaccine type A antigens than their sexmatched C57BL/6 counterparts, while that to B antigen did not differ between strains. In both strains IgG responses against type A vaccine antigens were greater in females than in males. The greater IgG responses correlated with lower splenic Tfr/Tfh and Tfr/GC B cell ratios and greater vaccine antigen-specific proliferative responses of CD4+ and B cells in splenocyte cultures. In both mouse strains IgG2a(c)/IgG1 ratios were comparable between sexes, but lower in BALB/c than in C57BL/6 mice indicating a shift in Th1/Th2 balance towards Th2 response in BALB/c ones. Consistently, splenocytes from BALB/c mice produced more IL-4 and less IFN-gamma than those from C57BL/6 mice. Significance: The study indicated that magnitude of humoral response to influenza type A haemagglutinins depends on mouse strain and sex, and thereby set background for the vaccination strategies taking into account biological sex, and in a longterm perspective individual differences in immune reactivity.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Mouse strain and sex as determinants of immune response to trivalent influenza vaccine
EP  - 126
SP  - 117
VL  - 207
DO  - 10.1016/j.lfs.2018.05.056
UR  - conv_437
ER  - 
@article{
author = "Petrović, Raisa and Bufan, Biljana and Arsenović-Ranin, Nevena and Živković, Irena and Minić, Rajna and Radojević, Katarina and Leposavić, Gordana",
year = "2018",
abstract = "Aims: The study examined the influence of sex and mouse strain on germinal center (GC) reaction and antibody responses to seasonal split trivalent influenza vaccine (TIV). Main methods: C57BL/6 and BALB/c mice of both sexes were immunized with TIV and examined for specific antibody response by ELISA. Splenic T follicular regulatory (Tfr), T follicular helper (Tfh) and GC B cells are detected by flow cytometry. The proliferative response of splenocytes, and concentrations of IFN-gamma and IL-4 upon restimulation with vaccine antigens were examined by 7-AAD staining and ELISA, respectively. Key findings: BALB/c mice developed more robust IgG responses to vaccine type A antigens than their sexmatched C57BL/6 counterparts, while that to B antigen did not differ between strains. In both strains IgG responses against type A vaccine antigens were greater in females than in males. The greater IgG responses correlated with lower splenic Tfr/Tfh and Tfr/GC B cell ratios and greater vaccine antigen-specific proliferative responses of CD4+ and B cells in splenocyte cultures. In both mouse strains IgG2a(c)/IgG1 ratios were comparable between sexes, but lower in BALB/c than in C57BL/6 mice indicating a shift in Th1/Th2 balance towards Th2 response in BALB/c ones. Consistently, splenocytes from BALB/c mice produced more IL-4 and less IFN-gamma than those from C57BL/6 mice. Significance: The study indicated that magnitude of humoral response to influenza type A haemagglutinins depends on mouse strain and sex, and thereby set background for the vaccination strategies taking into account biological sex, and in a longterm perspective individual differences in immune reactivity.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Mouse strain and sex as determinants of immune response to trivalent influenza vaccine",
pages = "126-117",
volume = "207",
doi = "10.1016/j.lfs.2018.05.056",
url = "conv_437"
}
Petrović, R., Bufan, B., Arsenović-Ranin, N., Živković, I., Minić, R., Radojević, K.,& Leposavić, G.. (2018). Mouse strain and sex as determinants of immune response to trivalent influenza vaccine. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 207, 117-126.
https://doi.org/10.1016/j.lfs.2018.05.056
conv_437
Petrović R, Bufan B, Arsenović-Ranin N, Živković I, Minić R, Radojević K, Leposavić G. Mouse strain and sex as determinants of immune response to trivalent influenza vaccine. in Life Sciences. 2018;207:117-126.
doi:10.1016/j.lfs.2018.05.056
conv_437 .
Petrović, Raisa, Bufan, Biljana, Arsenović-Ranin, Nevena, Živković, Irena, Minić, Rajna, Radojević, Katarina, Leposavić, Gordana, "Mouse strain and sex as determinants of immune response to trivalent influenza vaccine" in Life Sciences, 207 (2018):117-126,
https://doi.org/10.1016/j.lfs.2018.05.056 .,
conv_437 .
11
9
12

Application of the 3r concept in the production of European antiviperinum on horses - multisite, low volumes immunization protocol and Elisa

Milovanović, Vladimir; Dimitrijević, Ljiljana; Petrušić, Vladimir; Kadrić, Jasminka; Minić, Rajna; Živković, Irena

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2018)

TY  - JOUR
AU  - Milovanović, Vladimir
AU  - Dimitrijević, Ljiljana
AU  - Petrušić, Vladimir
AU  - Kadrić, Jasminka
AU  - Minić, Rajna
AU  - Živković, Irena
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/500
AB  - During time, both professionals and general public became aware of the importance of animal welfare. This term not only covers endangered wild animal species, animals used in food industry, pets and experimental animals, but also animals used in production of biologics. The implementation of the 3R concept (Replacement, Reduction and Refinement) is especially important in this type of production. In this article, we describe for the first time the low dose, low volume and multi-site immunization protocol, as well as appropriate ELISA we developed for production of European anti-viper (V. ammodytes, long horned) antivenom in horses, which can help to significantly improve the welfare of the used animals.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Application of the 3r concept in the production of European antiviperinum on horses - multisite, low volumes immunization protocol and Elisa
EP  - 419
IS  - 4
SP  - 401
VL  - 68
DO  - 10.2478/acve-2018-0033
UR  - conv_446
ER  - 
@article{
author = "Milovanović, Vladimir and Dimitrijević, Ljiljana and Petrušić, Vladimir and Kadrić, Jasminka and Minić, Rajna and Živković, Irena",
year = "2018",
abstract = "During time, both professionals and general public became aware of the importance of animal welfare. This term not only covers endangered wild animal species, animals used in food industry, pets and experimental animals, but also animals used in production of biologics. The implementation of the 3R concept (Replacement, Reduction and Refinement) is especially important in this type of production. In this article, we describe for the first time the low dose, low volume and multi-site immunization protocol, as well as appropriate ELISA we developed for production of European anti-viper (V. ammodytes, long horned) antivenom in horses, which can help to significantly improve the welfare of the used animals.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Application of the 3r concept in the production of European antiviperinum on horses - multisite, low volumes immunization protocol and Elisa",
pages = "419-401",
number = "4",
volume = "68",
doi = "10.2478/acve-2018-0033",
url = "conv_446"
}
Milovanović, V., Dimitrijević, L., Petrušić, V., Kadrić, J., Minić, R.,& Živković, I.. (2018). Application of the 3r concept in the production of European antiviperinum on horses - multisite, low volumes immunization protocol and Elisa. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(4), 401-419.
https://doi.org/10.2478/acve-2018-0033
conv_446
Milovanović V, Dimitrijević L, Petrušić V, Kadrić J, Minić R, Živković I. Application of the 3r concept in the production of European antiviperinum on horses - multisite, low volumes immunization protocol and Elisa. in Acta veterinaria - Beograd. 2018;68(4):401-419.
doi:10.2478/acve-2018-0033
conv_446 .
Milovanović, Vladimir, Dimitrijević, Ljiljana, Petrušić, Vladimir, Kadrić, Jasminka, Minić, Rajna, Živković, Irena, "Application of the 3r concept in the production of European antiviperinum on horses - multisite, low volumes immunization protocol and Elisa" in Acta veterinaria - Beograd, 68, no. 4 (2018):401-419,
https://doi.org/10.2478/acve-2018-0033 .,
conv_446 .
7
3
4

Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy

Mrkić, Ivan; Minić, Rajna; Popović, Dragan; Živković, Irena; Gavrović-Jankulović, Marija

(Pergamon-Elsevier Science Ltd, Oxford, 2018)

TY  - JOUR
AU  - Mrkić, Ivan
AU  - Minić, Rajna
AU  - Popović, Dragan
AU  - Živković, Irena
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/515
AB  - Aim To investigate the immunomodulatory potential of a chimera composed of the receptor-binding domain of hemagglutinin 1 (H1s) from Influenza virus and Der p 2 (D2) allergen for allergen-specific immunotherapy of house-dust mite allergy (HDM). Main methods: H1sD2 chimera and D2 allergen were produced by genetic engineering in E. coli. Recombinant antigens were extracted from inclusion bodies by urea, then refolded and purified by immobilized-metal affinity chromatography (IMAC). Purity was verified by 2D-PAGE and secondary structures were assessed by CD spectroscopy. IgE reactivity of H1sD2 and D2 was tested in western blot with sera from 8 persons with clinical history of HDM allergy. Immunogenicity of H1sD2 and D2 were analyzed in Balb/c mice. Cytokine profile was analyzed by ELISA after stimulation of mouse spleen cells with H1sD2 and D2. Leukocyte population abundance of cells isolated from spleen and lymph node was assessed by flow cytometry. Key findings: Purified recombinant proteins H1sD2 (42 kDa) and D2 (15 kDa) revealed well defined secondary structures, and preserved IgE reactive epitopes. Analysis of supernatants of mouse spleen cells after stimulation with H1sD2 and D2, revealed a qualitatively different cytokine profile from H1sD2 immunized mouse cells (increase in IL10). CD8+ cells were decreased in the lymph node of D2 immunized mice, whereas H1sD2 immunization led to an increase of CD8+ cells in both the lymph node and the spleen. Significance: H1sD2 chimera attenuates Der p 2-inherent Th2 response and directs the immune response toward Th1 and Treg phenotype.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy
EP  - 165
SP  - 158
VL  - 213
DO  - 10.1016/j.lfs.2018.10.036
UR  - conv_443
ER  - 
@article{
author = "Mrkić, Ivan and Minić, Rajna and Popović, Dragan and Živković, Irena and Gavrović-Jankulović, Marija",
year = "2018",
abstract = "Aim To investigate the immunomodulatory potential of a chimera composed of the receptor-binding domain of hemagglutinin 1 (H1s) from Influenza virus and Der p 2 (D2) allergen for allergen-specific immunotherapy of house-dust mite allergy (HDM). Main methods: H1sD2 chimera and D2 allergen were produced by genetic engineering in E. coli. Recombinant antigens were extracted from inclusion bodies by urea, then refolded and purified by immobilized-metal affinity chromatography (IMAC). Purity was verified by 2D-PAGE and secondary structures were assessed by CD spectroscopy. IgE reactivity of H1sD2 and D2 was tested in western blot with sera from 8 persons with clinical history of HDM allergy. Immunogenicity of H1sD2 and D2 were analyzed in Balb/c mice. Cytokine profile was analyzed by ELISA after stimulation of mouse spleen cells with H1sD2 and D2. Leukocyte population abundance of cells isolated from spleen and lymph node was assessed by flow cytometry. Key findings: Purified recombinant proteins H1sD2 (42 kDa) and D2 (15 kDa) revealed well defined secondary structures, and preserved IgE reactive epitopes. Analysis of supernatants of mouse spleen cells after stimulation with H1sD2 and D2, revealed a qualitatively different cytokine profile from H1sD2 immunized mouse cells (increase in IL10). CD8+ cells were decreased in the lymph node of D2 immunized mice, whereas H1sD2 immunization led to an increase of CD8+ cells in both the lymph node and the spleen. Significance: H1sD2 chimera attenuates Der p 2-inherent Th2 response and directs the immune response toward Th1 and Treg phenotype.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy",
pages = "165-158",
volume = "213",
doi = "10.1016/j.lfs.2018.10.036",
url = "conv_443"
}
Mrkić, I., Minić, R., Popović, D., Živković, I.,& Gavrović-Jankulović, M.. (2018). Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 213, 158-165.
https://doi.org/10.1016/j.lfs.2018.10.036
conv_443
Mrkić I, Minić R, Popović D, Živković I, Gavrović-Jankulović M. Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy. in Life Sciences. 2018;213:158-165.
doi:10.1016/j.lfs.2018.10.036
conv_443 .
Mrkić, Ivan, Minić, Rajna, Popović, Dragan, Živković, Irena, Gavrović-Jankulović, Marija, "Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy" in Life Sciences, 213 (2018):158-165,
https://doi.org/10.1016/j.lfs.2018.10.036 .,
conv_443 .
2
2
2

Lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial

Michalickova, Danica; Kostić-Vučićević, Marija; Vukašinović-Vesić, Milica; Stojmenović, Tamara; Dikić, Nenad; Anđelković, Marija; Đorđević, Brižita; Tanasković, Bojana P.; Minić, Rajna

(Lippincott Williams & Wilkins, Philadelphia, 2017)

TY  - JOUR
AU  - Michalickova, Danica
AU  - Kostić-Vučićević, Marija
AU  - Vukašinović-Vesić, Milica
AU  - Stojmenović, Tamara
AU  - Dikić, Nenad
AU  - Anđelković, Marija
AU  - Đorđević, Brižita
AU  - Tanasković, Bojana P.
AU  - Minić, Rajna
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/491
AB  - To test the influence of probiotic supplementation on humoral immune response, a double-blind, placebo-controlled trial was conducted. Thirty athletes (24 males and 6 females, females: (V)over dotO(2)max 38.2 +/- 4.9 ml.kg(-1).min(-1), age 23.2 +/- 1.4 years; males: (V)over dotO(2)max 57.5 +/- 9.2 ml.kg(-1).min(-1), age 24.0 +/- 2.4 years, mean +/- SD) were randomized either to the probiotic group (Lactobacillus helveticus Lafti L10, 2 x 10(10) colony-forming units) or to the placebo group. Serum and saliva samples were collected at the baseline and after 14 weeks. Total and specific antibacterial antibody levels of IgM, IgG, and IgA classes were determined for different bacteria in the serum, and in saliva, total and specific antibacterial IgA levels were examined. Total IgM was elevated in both probiotic (18%, 15-20%; mean, 90% confidence interval; p = 0.02) and placebo group (35%, 22-47%; p = 0.02), without observed differences in changes between the groups. No significant changes in IgM levels specific for tested bacteria were found. Total IgG level was constant in both groups. A significant (16%, 22.8 to 35%, p = 0.04) reduction of anti-Enterococcus faecalis IgG was noted in the placebo group, in comparison with the probiotic group. There was a substantial decrease in total IgA level in the placebo group, when measured either in serum (15%, 12-18%, p = 0.04) or in saliva (35%, -1.4 to 53%, p = 0.03). Significantly reduced levels of serum anti-lactic acid bacteria IgA antibodies in the placebo group compared with the probiotic group were detected for Lactobacillus rhamnosus LA68 (24%, 5.8-42%, p = 0.02) and for L. rhamnosus LB64 (15%, 2.7-27%, p = 0.02). Probiotic administration could have beneficial effects on systemic humoral and mucosal immune responses.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Strength and Conditioning Research
T1  - Lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial
EP  - 70
IS  - 1
SP  - 62
VL  - 31
DO  - 10.1519/JSC.0000000000001456
UR  - conv_400
ER  - 
@article{
author = "Michalickova, Danica and Kostić-Vučićević, Marija and Vukašinović-Vesić, Milica and Stojmenović, Tamara and Dikić, Nenad and Anđelković, Marija and Đorđević, Brižita and Tanasković, Bojana P. and Minić, Rajna",
year = "2017",
abstract = "To test the influence of probiotic supplementation on humoral immune response, a double-blind, placebo-controlled trial was conducted. Thirty athletes (24 males and 6 females, females: (V)over dotO(2)max 38.2 +/- 4.9 ml.kg(-1).min(-1), age 23.2 +/- 1.4 years; males: (V)over dotO(2)max 57.5 +/- 9.2 ml.kg(-1).min(-1), age 24.0 +/- 2.4 years, mean +/- SD) were randomized either to the probiotic group (Lactobacillus helveticus Lafti L10, 2 x 10(10) colony-forming units) or to the placebo group. Serum and saliva samples were collected at the baseline and after 14 weeks. Total and specific antibacterial antibody levels of IgM, IgG, and IgA classes were determined for different bacteria in the serum, and in saliva, total and specific antibacterial IgA levels were examined. Total IgM was elevated in both probiotic (18%, 15-20%; mean, 90% confidence interval; p = 0.02) and placebo group (35%, 22-47%; p = 0.02), without observed differences in changes between the groups. No significant changes in IgM levels specific for tested bacteria were found. Total IgG level was constant in both groups. A significant (16%, 22.8 to 35%, p = 0.04) reduction of anti-Enterococcus faecalis IgG was noted in the placebo group, in comparison with the probiotic group. There was a substantial decrease in total IgA level in the placebo group, when measured either in serum (15%, 12-18%, p = 0.04) or in saliva (35%, -1.4 to 53%, p = 0.03). Significantly reduced levels of serum anti-lactic acid bacteria IgA antibodies in the placebo group compared with the probiotic group were detected for Lactobacillus rhamnosus LA68 (24%, 5.8-42%, p = 0.02) and for L. rhamnosus LB64 (15%, 2.7-27%, p = 0.02). Probiotic administration could have beneficial effects on systemic humoral and mucosal immune responses.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Strength and Conditioning Research",
title = "Lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial",
pages = "70-62",
number = "1",
volume = "31",
doi = "10.1519/JSC.0000000000001456",
url = "conv_400"
}
Michalickova, D., Kostić-Vučićević, M., Vukašinović-Vesić, M., Stojmenović, T., Dikić, N., Anđelković, M., Đorđević, B., Tanasković, B. P.,& Minić, R.. (2017). Lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial. in Journal of Strength and Conditioning Research
Lippincott Williams & Wilkins, Philadelphia., 31(1), 62-70.
https://doi.org/10.1519/JSC.0000000000001456
conv_400
Michalickova D, Kostić-Vučićević M, Vukašinović-Vesić M, Stojmenović T, Dikić N, Anđelković M, Đorđević B, Tanasković BP, Minić R. Lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial. in Journal of Strength and Conditioning Research. 2017;31(1):62-70.
doi:10.1519/JSC.0000000000001456
conv_400 .
Michalickova, Danica, Kostić-Vučićević, Marija, Vukašinović-Vesić, Milica, Stojmenović, Tamara, Dikić, Nenad, Anđelković, Marija, Đorđević, Brižita, Tanasković, Bojana P., Minić, Rajna, "Lactobacillus helveticus lafti l10 supplementation modulates mucosal and humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial" in Journal of Strength and Conditioning Research, 31, no. 1 (2017):62-70,
https://doi.org/10.1519/JSC.0000000000001456 .,
conv_400 .
20
17
15
15

Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter

Stanković, Ivan; Michalickova, Danica; Minić, Rajna; Kotur-Stevuljević, Jelena; Miljković, Milica; Kostić-Vučićević, Marija; Dikić, Nenad; Anđelković, Marija; Baralić, Ivana; Ivanović, Nevena; Đorđević, Brižita

(Lippincott Williams & Wilkins, Philadelphia, 2017)

TY  - CONF
AU  - Stanković, Ivan
AU  - Michalickova, Danica
AU  - Minić, Rajna
AU  - Kotur-Stevuljević, Jelena
AU  - Miljković, Milica
AU  - Kostić-Vučićević, Marija
AU  - Dikić, Nenad
AU  - Anđelković, Marija
AU  - Baralić, Ivana
AU  - Ivanović, Nevena
AU  - Đorđević, Brižita
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/481
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Medicine and Science in Sports and Exercise
T1  - Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter
EP  - 457
IS  - 5
SP  - 457
VL  - 49
DO  - 10.1249/01.mss.0000518140.98604.f5
UR  - conv_420
ER  - 
@conference{
author = "Stanković, Ivan and Michalickova, Danica and Minić, Rajna and Kotur-Stevuljević, Jelena and Miljković, Milica and Kostić-Vučićević, Marija and Dikić, Nenad and Anđelković, Marija and Baralić, Ivana and Ivanović, Nevena and Đorđević, Brižita",
year = "2017",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Medicine and Science in Sports and Exercise",
title = "Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter",
pages = "457-457",
number = "5",
volume = "49",
doi = "10.1249/01.mss.0000518140.98604.f5",
url = "conv_420"
}
Stanković, I., Michalickova, D., Minić, R., Kotur-Stevuljević, J., Miljković, M., Kostić-Vučićević, M., Dikić, N., Anđelković, M., Baralić, I., Ivanović, N.,& Đorđević, B.. (2017). Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter. in Medicine and Science in Sports and Exercise
Lippincott Williams & Wilkins, Philadelphia., 49(5), 457-457.
https://doi.org/10.1249/01.mss.0000518140.98604.f5
conv_420
Stanković I, Michalickova D, Minić R, Kotur-Stevuljević J, Miljković M, Kostić-Vučićević M, Dikić N, Anđelković M, Baralić I, Ivanović N, Đorđević B. Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter. in Medicine and Science in Sports and Exercise. 2017;49(5):457-457.
doi:10.1249/01.mss.0000518140.98604.f5
conv_420 .
Stanković, Ivan, Michalickova, Danica, Minić, Rajna, Kotur-Stevuljević, Jelena, Miljković, Milica, Kostić-Vučićević, Marija, Dikić, Nenad, Anđelković, Marija, Baralić, Ivana, Ivanović, Nevena, Đorđević, Brižita, "Changes In Parameters Of Immunological And Oxidative Status In Elite Athletes During Winter" in Medicine and Science in Sports and Exercise, 49, no. 5 (2017):457-457,
https://doi.org/10.1249/01.mss.0000518140.98604.f5 .,
conv_420 .

Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens

Kuczkowska, Katarzyna; Kleiveland, Charlotte R.; Minić, Rajna; Moen, Lars F.; Overland, Lise; Tjaland, Rannei; Carlsen, Harald; Lea, Tor; Mathiesen, Geir; Eijsink, Vincent G.H.

(Amer Soc Microbiology, Washington, 2017)

TY  - JOUR
AU  - Kuczkowska, Katarzyna
AU  - Kleiveland, Charlotte R.
AU  - Minić, Rajna
AU  - Moen, Lars F.
AU  - Overland, Lise
AU  - Tjaland, Rannei
AU  - Carlsen, Harald
AU  - Lea, Tor
AU  - Mathiesen, Geir
AU  - Eijsink, Vincent G.H.
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/492
AB  - Tuberculosis (TB) remains among the most deadly diseases in the world. The only available vaccine against tuberculosis is the bacille Calmette-Guerin (BCG) vaccine, which does not ensure full protection in adults. There is a global urgency for the development of an effective vaccine for preventing disease transmission, and it requires novel approaches. We are exploring the use of lactic acid bacteria (LAB) as a vector for antigen delivery to mucosal sites. Here, we demonstrate the successful expression and surface display of a Mycobacterium tuberculosis fusion antigen (comprising Ag85B and ESAT-6, referred to as AgE6) on Lactobacillus plantarum. The AgE6 fusion antigen was targeted to the bacterial surface using two different anchors, a lipoprotein anchor directing the protein to the cell membrane and a covalent cell wall anchor. AgE6-producing L. plantarum strains using each of the two anchors induced antigen-specific proliferative responses in lymphocytes purified from TB-positive donors. Similarly, both strains induced immune responses in mice after nasal or oral immunization. The impact of the anchoring strategies was reflected in dissimilarities in the immune responses generated by the two L. plantarum strains in vivo. The present study comprises an initial step toward the development of L. plantarum as a vector for M. tuberculosis antigen delivery. IMPORTANCE This work presents the development of Lactobacillus plantarum as a candidate mucosal vaccine against tuberculosis. Tuberculosis remains one of the top infectious diseases worldwide, and the only available vaccine, bacille Calmette-Guerin (BCG), fails to protect adults and adolescents. Direct antigen delivery to mucosal sites is a promising strategy in tuberculosis vaccine development, and lactic acid bacteria potentially provide easy, safe, and low-cost delivery vehicles for mucosal immunization. We have engineered L. plantarum strains to produce a Mycobacterium tuberculosis fusion antigen and to anchor this antigen to the bacterial cell wall or to the cell membrane. The recombinant strains elicited proliferative antigenspecific T-cell responses in white blood cells from tuberculosis-positive humans and induced specific immune responses after nasal and oral administrations in mice.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens
IS  - 2
VL  - 83
DO  - 10.1128/AEM.02782-16
UR  - conv_403
ER  - 
@article{
author = "Kuczkowska, Katarzyna and Kleiveland, Charlotte R. and Minić, Rajna and Moen, Lars F. and Overland, Lise and Tjaland, Rannei and Carlsen, Harald and Lea, Tor and Mathiesen, Geir and Eijsink, Vincent G.H.",
year = "2017",
abstract = "Tuberculosis (TB) remains among the most deadly diseases in the world. The only available vaccine against tuberculosis is the bacille Calmette-Guerin (BCG) vaccine, which does not ensure full protection in adults. There is a global urgency for the development of an effective vaccine for preventing disease transmission, and it requires novel approaches. We are exploring the use of lactic acid bacteria (LAB) as a vector for antigen delivery to mucosal sites. Here, we demonstrate the successful expression and surface display of a Mycobacterium tuberculosis fusion antigen (comprising Ag85B and ESAT-6, referred to as AgE6) on Lactobacillus plantarum. The AgE6 fusion antigen was targeted to the bacterial surface using two different anchors, a lipoprotein anchor directing the protein to the cell membrane and a covalent cell wall anchor. AgE6-producing L. plantarum strains using each of the two anchors induced antigen-specific proliferative responses in lymphocytes purified from TB-positive donors. Similarly, both strains induced immune responses in mice after nasal or oral immunization. The impact of the anchoring strategies was reflected in dissimilarities in the immune responses generated by the two L. plantarum strains in vivo. The present study comprises an initial step toward the development of L. plantarum as a vector for M. tuberculosis antigen delivery. IMPORTANCE This work presents the development of Lactobacillus plantarum as a candidate mucosal vaccine against tuberculosis. Tuberculosis remains one of the top infectious diseases worldwide, and the only available vaccine, bacille Calmette-Guerin (BCG), fails to protect adults and adolescents. Direct antigen delivery to mucosal sites is a promising strategy in tuberculosis vaccine development, and lactic acid bacteria potentially provide easy, safe, and low-cost delivery vehicles for mucosal immunization. We have engineered L. plantarum strains to produce a Mycobacterium tuberculosis fusion antigen and to anchor this antigen to the bacterial cell wall or to the cell membrane. The recombinant strains elicited proliferative antigenspecific T-cell responses in white blood cells from tuberculosis-positive humans and induced specific immune responses after nasal and oral administrations in mice.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens",
number = "2",
volume = "83",
doi = "10.1128/AEM.02782-16",
url = "conv_403"
}
Kuczkowska, K., Kleiveland, C. R., Minić, R., Moen, L. F., Overland, L., Tjaland, R., Carlsen, H., Lea, T., Mathiesen, G.,& Eijsink, V. G.H.. (2017). Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 83(2).
https://doi.org/10.1128/AEM.02782-16
conv_403
Kuczkowska K, Kleiveland CR, Minić R, Moen LF, Overland L, Tjaland R, Carlsen H, Lea T, Mathiesen G, Eijsink VG. Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens. in Applied and Environmental Microbiology. 2017;83(2).
doi:10.1128/AEM.02782-16
conv_403 .
Kuczkowska, Katarzyna, Kleiveland, Charlotte R., Minić, Rajna, Moen, Lars F., Overland, Lise, Tjaland, Rannei, Carlsen, Harald, Lea, Tor, Mathiesen, Geir, Eijsink, Vincent G.H., "Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens" in Applied and Environmental Microbiology, 83, no. 2 (2017),
https://doi.org/10.1128/AEM.02782-16 .,
conv_403 .
3
30
24
27

Modulation of the specific immune response in Balb/cmice by intranasal application of recombinant H1D2 chimera

Mrkić, Ivan; Minić, Rajna; Bulat, Tanja; Aradska, Jana; Atanasković-Marković, Marina; Drakulić, Branko; Gavrović-Jankulović, Marija

(Wiley, Hoboken, 2017)

TY  - JOUR
AU  - Mrkić, Ivan
AU  - Minić, Rajna
AU  - Bulat, Tanja
AU  - Aradska, Jana
AU  - Atanasković-Marković, Marina
AU  - Drakulić, Branko
AU  - Gavrović-Jankulović, Marija
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/485
AB  - BACKGROUND: Group 1 and group 2 allergens from house dust mite are the major elicitors of respiratory allergic diseases and the main candidates for immunotherapy. RESULTS: The potential therapeutic role of a chimera composed of recombinant Der p 2 (D2) linked to Influenza A virus hemagglutinin 1 (H1) for intranasal application was created, expressed and tested in a mouse model. H1D2 and D2 were produced by genetic engineering in Escherichia coli and their primary structure was confirmed by mass fingerprint. Both antigens preserved IgE reactivity in immunoblot with serum from seven house dust mite allergic persons. Balb/c mice were sensitized with D2 allergen in alum and subsequently received H1D2 or D2, intranasally. The reduced levels of serum D2 specific IgE, together with the increased serum specific IgG and IgA were detected in both groups which received H1D2 and D2 intranasally. A higher level of effector CD4+CD25+ spleen lymphocytes was found only in the group of mice which received i.n. H1D2. CONCLUSION: H1D2 chimera can have therapeutic potential in Der p 2 allergic persons as dual vaccine which, beside protective allergen specific, can provide protective antibodies against Influenza A virus hemagglutinin 1. (C) 2016 Society of Chemical Industry
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Modulation of the specific immune response in Balb/cmice by intranasal application of recombinant H1D2 chimera
EP  - 1335
IS  - 6
SP  - 1328
VL  - 92
DO  - 10.1002/jctb.5127
UR  - conv_413
ER  - 
@article{
author = "Mrkić, Ivan and Minić, Rajna and Bulat, Tanja and Aradska, Jana and Atanasković-Marković, Marina and Drakulić, Branko and Gavrović-Jankulović, Marija",
year = "2017",
abstract = "BACKGROUND: Group 1 and group 2 allergens from house dust mite are the major elicitors of respiratory allergic diseases and the main candidates for immunotherapy. RESULTS: The potential therapeutic role of a chimera composed of recombinant Der p 2 (D2) linked to Influenza A virus hemagglutinin 1 (H1) for intranasal application was created, expressed and tested in a mouse model. H1D2 and D2 were produced by genetic engineering in Escherichia coli and their primary structure was confirmed by mass fingerprint. Both antigens preserved IgE reactivity in immunoblot with serum from seven house dust mite allergic persons. Balb/c mice were sensitized with D2 allergen in alum and subsequently received H1D2 or D2, intranasally. The reduced levels of serum D2 specific IgE, together with the increased serum specific IgG and IgA were detected in both groups which received H1D2 and D2 intranasally. A higher level of effector CD4+CD25+ spleen lymphocytes was found only in the group of mice which received i.n. H1D2. CONCLUSION: H1D2 chimera can have therapeutic potential in Der p 2 allergic persons as dual vaccine which, beside protective allergen specific, can provide protective antibodies against Influenza A virus hemagglutinin 1. (C) 2016 Society of Chemical Industry",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Modulation of the specific immune response in Balb/cmice by intranasal application of recombinant H1D2 chimera",
pages = "1335-1328",
number = "6",
volume = "92",
doi = "10.1002/jctb.5127",
url = "conv_413"
}
Mrkić, I., Minić, R., Bulat, T., Aradska, J., Atanasković-Marković, M., Drakulić, B.,& Gavrović-Jankulović, M.. (2017). Modulation of the specific immune response in Balb/cmice by intranasal application of recombinant H1D2 chimera. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 92(6), 1328-1335.
https://doi.org/10.1002/jctb.5127
conv_413
Mrkić I, Minić R, Bulat T, Aradska J, Atanasković-Marković M, Drakulić B, Gavrović-Jankulović M. Modulation of the specific immune response in Balb/cmice by intranasal application of recombinant H1D2 chimera. in Journal of Chemical Technology and Biotechnology. 2017;92(6):1328-1335.
doi:10.1002/jctb.5127
conv_413 .
Mrkić, Ivan, Minić, Rajna, Bulat, Tanja, Aradska, Jana, Atanasković-Marković, Marina, Drakulić, Branko, Gavrović-Jankulović, Marija, "Modulation of the specific immune response in Balb/cmice by intranasal application of recombinant H1D2 chimera" in Journal of Chemical Technology and Biotechnology, 92, no. 6 (2017):1328-1335,
https://doi.org/10.1002/jctb.5127 .,
conv_413 .
2
2
2

Characterization of Intor:Swiss albino mice adopted in the Institute of virology, vaccines and sera: Torlak, Belgrade in the early twentieth century

Živković, Irena; Rajnpreht, Irena; Minić, Rajna; Mitić, Katarina; Aleksić, Iva; Kadrić, Jasminka; Petrušić, Vladimir

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2016)

TY  - JOUR
AU  - Živković, Irena
AU  - Rajnpreht, Irena
AU  - Minić, Rajna
AU  - Mitić, Katarina
AU  - Aleksić, Iva
AU  - Kadrić, Jasminka
AU  - Petrušić, Vladimir
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/461
AB  - The Institute of Virology, Vaccines and Sera Torlak was established in 1927, while the first vaccine was produced in the Institute in 1930. Vaccines production implies using experimental animals, including mice, in in-process controls. The laboratory mice which have been in use in Torlak Institute from the very beginning belong to Swiss albino outbred stock. This stock, which has been in use for more than 80 years contains a large number of mice maintained at all times, was recently named Intor:Swiss. Biological characteristics of Intor:Swiss stock, are presented in this paper for the first time. Taking into account the presented characteristics, the Institute Torlak's Swiss mice are suitable for use in pharmaceutical studies, vaccine development research and basic research, as well as in toxicological studies. The publication of data on the Intor:Swiss mice represents a contribution to the international scientific community, since it offers the possibility for obtaining an additional outbred mouse stock for research.
AB  - Institut za Virusologiju, vakcine i serume Torlak, osnovan je 1927., a prva vakcina u Institutu proizvedena je 1930. Proizvodnja vakcina je složen proces koji između ostalog podrazumeva i korišćenje eksperimentalnih životinja u kontroli samog procesa. Laboratorijski miševi koji su od samog početka bili u upotrebi u Institutu Torlak, pripadaju Swiss albino outbred soju. Ova kolonija je u upotrebi više od 80 godina i sve vreme se sastoji od velikog broja jedinki što omogućava očuvanje genetske raznolikosti, pa samim tim i outbred karakteristika. Ovi miševi su odnedavno registrovani pod imenom Intor:Swiss, i njihove biološke osobine su u ovom radu prikazane po prvi put. Swiss miševi Instituta Torlak pogodni su za upotrebu u farmaceutskim studijama, za razvojno istraživanje vakcina, osnovna istraživanja i toksikološka ispitivanja. Zbog svega navedenog Intor:Swiss miševi predstavljaju još jedan pogodan animalni model za ispitivanje lekova i vakcina.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Characterization of Intor:Swiss albino mice adopted in the Institute of virology, vaccines and sera: Torlak, Belgrade in the early twentieth century
T1  - Karakterizacija Intor:Swiss soja albino miševa donetog u Institut za virusologiju, vakcine i serume - Torlak početkom XX veka
EP  - 293
IS  - 3
SP  - 279
VL  - 66
DO  - 10.1515/acve-2016-0025
UR  - conv_63
ER  - 
@article{
author = "Živković, Irena and Rajnpreht, Irena and Minić, Rajna and Mitić, Katarina and Aleksić, Iva and Kadrić, Jasminka and Petrušić, Vladimir",
year = "2016",
abstract = "The Institute of Virology, Vaccines and Sera Torlak was established in 1927, while the first vaccine was produced in the Institute in 1930. Vaccines production implies using experimental animals, including mice, in in-process controls. The laboratory mice which have been in use in Torlak Institute from the very beginning belong to Swiss albino outbred stock. This stock, which has been in use for more than 80 years contains a large number of mice maintained at all times, was recently named Intor:Swiss. Biological characteristics of Intor:Swiss stock, are presented in this paper for the first time. Taking into account the presented characteristics, the Institute Torlak's Swiss mice are suitable for use in pharmaceutical studies, vaccine development research and basic research, as well as in toxicological studies. The publication of data on the Intor:Swiss mice represents a contribution to the international scientific community, since it offers the possibility for obtaining an additional outbred mouse stock for research., Institut za Virusologiju, vakcine i serume Torlak, osnovan je 1927., a prva vakcina u Institutu proizvedena je 1930. Proizvodnja vakcina je složen proces koji između ostalog podrazumeva i korišćenje eksperimentalnih životinja u kontroli samog procesa. Laboratorijski miševi koji su od samog početka bili u upotrebi u Institutu Torlak, pripadaju Swiss albino outbred soju. Ova kolonija je u upotrebi više od 80 godina i sve vreme se sastoji od velikog broja jedinki što omogućava očuvanje genetske raznolikosti, pa samim tim i outbred karakteristika. Ovi miševi su odnedavno registrovani pod imenom Intor:Swiss, i njihove biološke osobine su u ovom radu prikazane po prvi put. Swiss miševi Instituta Torlak pogodni su za upotrebu u farmaceutskim studijama, za razvojno istraživanje vakcina, osnovna istraživanja i toksikološka ispitivanja. Zbog svega navedenog Intor:Swiss miševi predstavljaju još jedan pogodan animalni model za ispitivanje lekova i vakcina.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Characterization of Intor:Swiss albino mice adopted in the Institute of virology, vaccines and sera: Torlak, Belgrade in the early twentieth century, Karakterizacija Intor:Swiss soja albino miševa donetog u Institut za virusologiju, vakcine i serume - Torlak početkom XX veka",
pages = "293-279",
number = "3",
volume = "66",
doi = "10.1515/acve-2016-0025",
url = "conv_63"
}
Živković, I., Rajnpreht, I., Minić, R., Mitić, K., Aleksić, I., Kadrić, J.,& Petrušić, V.. (2016). Characterization of Intor:Swiss albino mice adopted in the Institute of virology, vaccines and sera: Torlak, Belgrade in the early twentieth century. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 279-293.
https://doi.org/10.1515/acve-2016-0025
conv_63
Živković I, Rajnpreht I, Minić R, Mitić K, Aleksić I, Kadrić J, Petrušić V. Characterization of Intor:Swiss albino mice adopted in the Institute of virology, vaccines and sera: Torlak, Belgrade in the early twentieth century. in Acta veterinaria - Beograd. 2016;66(3):279-293.
doi:10.1515/acve-2016-0025
conv_63 .
Živković, Irena, Rajnpreht, Irena, Minić, Rajna, Mitić, Katarina, Aleksić, Iva, Kadrić, Jasminka, Petrušić, Vladimir, "Characterization of Intor:Swiss albino mice adopted in the Institute of virology, vaccines and sera: Torlak, Belgrade in the early twentieth century" in Acta veterinaria - Beograd, 66, no. 3 (2016):279-293,
https://doi.org/10.1515/acve-2016-0025 .,
conv_63 .
2
2
1

Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial

Michalickova, Danica; Minić, Rajna; Dikić, Nenad; Anđelković, Marija; Kostić-Vučićević, Marija; Stojmenović, Tamara; Nikolić, Ivan; Đorđević, Brižita

(Canadian Science Publishing, Ottawa, 2016)

TY  - JOUR
AU  - Michalickova, Danica
AU  - Minić, Rajna
AU  - Dikić, Nenad
AU  - Anđelković, Marija
AU  - Kostić-Vučićević, Marija
AU  - Stojmenović, Tamara
AU  - Nikolić, Ivan
AU  - Đorđević, Brižita
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/476
AB  - A randomized, double-blind, placebo-controlled study was conducted to evaluate if Lactobacillus helveticus Lafti L10 (Lallemand Health Solutions, Montreal, Que., Canada) supplementation during 14 weeks in winter can influence the duration, severity, and incidence of upper respiratory tract illness (URTI), as well as to monitor different immune parameters in the population of elite athletes. Before and after the treatment, cardiopulmonary testing and self-rated state of moods evaluation (by Profile of Mood States questionnaire) were performed and blood samples were collected. Thirty-nine elite athletes were randomized either to the placebo (n = 19) or the probiotic (n = 20) group. The probiotic group received L. helveticus Lafti L10, 2 x 10(10) Colony Forming Units. Lafti L10 significantly shortened the URTI episode duration (7.25 +/- 2.90 vs. 10.64 +/- 4.67 days, p = 0.047) and decreased the number of symptoms in the probiotic group (4.92 +/- 1.96 vs. 6.91 +/- 1.22, p = 0.035). Severity and incidence of URTI did not differ between the treatments. There were no significant changes in leukocyte subpopulation abundance, transforming growth factor-beta serum levels, level of interleukin-10 secreted from peptidoglican stimulated peripheral blood mononuclear cells (PBMCs), interferon-gamma level secreted from concanavalin A-stimulated PBMCs or viability/proliferation of PBMCs upon antigen stimulation. Group effect for CD4+/CD8+ ratio was significant (F-[1,F-37] = 6.99, p = 0.020, eta(2) = 0.350); this difference was not significant at baseline, but was evident after 14 weeks (p = 0.02). A significant interaction effect was noted for self-rated sense of vigor (F-[1,F-37] = 11.76, p = 0.009, eta(2) = 0.595). Self-rated sense of vigor increased in the probiotic group (18.5 +/- 4.1 vs. 21.0 +/- 2.6, p = 0.012). Probiotic strain Lafti L10 can be a beneficial nutritional supplement for the reduction of URTI length in elite athletes.
PB  - Canadian Science Publishing, Ottawa
T2  - Applied Physiology Nutrition and Metabolism
T1  - Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial
EP  - 789
IS  - 7
SP  - 782
VL  - 41
DO  - 10.1139/apnm-2015-0541
UR  - conv_390
ER  - 
@article{
author = "Michalickova, Danica and Minić, Rajna and Dikić, Nenad and Anđelković, Marija and Kostić-Vučićević, Marija and Stojmenović, Tamara and Nikolić, Ivan and Đorđević, Brižita",
year = "2016",
abstract = "A randomized, double-blind, placebo-controlled study was conducted to evaluate if Lactobacillus helveticus Lafti L10 (Lallemand Health Solutions, Montreal, Que., Canada) supplementation during 14 weeks in winter can influence the duration, severity, and incidence of upper respiratory tract illness (URTI), as well as to monitor different immune parameters in the population of elite athletes. Before and after the treatment, cardiopulmonary testing and self-rated state of moods evaluation (by Profile of Mood States questionnaire) were performed and blood samples were collected. Thirty-nine elite athletes were randomized either to the placebo (n = 19) or the probiotic (n = 20) group. The probiotic group received L. helveticus Lafti L10, 2 x 10(10) Colony Forming Units. Lafti L10 significantly shortened the URTI episode duration (7.25 +/- 2.90 vs. 10.64 +/- 4.67 days, p = 0.047) and decreased the number of symptoms in the probiotic group (4.92 +/- 1.96 vs. 6.91 +/- 1.22, p = 0.035). Severity and incidence of URTI did not differ between the treatments. There were no significant changes in leukocyte subpopulation abundance, transforming growth factor-beta serum levels, level of interleukin-10 secreted from peptidoglican stimulated peripheral blood mononuclear cells (PBMCs), interferon-gamma level secreted from concanavalin A-stimulated PBMCs or viability/proliferation of PBMCs upon antigen stimulation. Group effect for CD4+/CD8+ ratio was significant (F-[1,F-37] = 6.99, p = 0.020, eta(2) = 0.350); this difference was not significant at baseline, but was evident after 14 weeks (p = 0.02). A significant interaction effect was noted for self-rated sense of vigor (F-[1,F-37] = 11.76, p = 0.009, eta(2) = 0.595). Self-rated sense of vigor increased in the probiotic group (18.5 +/- 4.1 vs. 21.0 +/- 2.6, p = 0.012). Probiotic strain Lafti L10 can be a beneficial nutritional supplement for the reduction of URTI length in elite athletes.",
publisher = "Canadian Science Publishing, Ottawa",
journal = "Applied Physiology Nutrition and Metabolism",
title = "Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial",
pages = "789-782",
number = "7",
volume = "41",
doi = "10.1139/apnm-2015-0541",
url = "conv_390"
}
Michalickova, D., Minić, R., Dikić, N., Anđelković, M., Kostić-Vučićević, M., Stojmenović, T., Nikolić, I.,& Đorđević, B.. (2016). Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial. in Applied Physiology Nutrition and Metabolism
Canadian Science Publishing, Ottawa., 41(7), 782-789.
https://doi.org/10.1139/apnm-2015-0541
conv_390
Michalickova D, Minić R, Dikić N, Anđelković M, Kostić-Vučićević M, Stojmenović T, Nikolić I, Đorđević B. Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial. in Applied Physiology Nutrition and Metabolism. 2016;41(7):782-789.
doi:10.1139/apnm-2015-0541
conv_390 .
Michalickova, Danica, Minić, Rajna, Dikić, Nenad, Anđelković, Marija, Kostić-Vučićević, Marija, Stojmenović, Tamara, Nikolić, Ivan, Đorđević, Brižita, "Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial" in Applied Physiology Nutrition and Metabolism, 41, no. 7 (2016):782-789,
https://doi.org/10.1139/apnm-2015-0541 .,
conv_390 .
37
47
36
40

Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial

Michalickova, Danica; Minić, Rajna; Dikić, Nenad; Anđelković, Marija; Kostić-Vučićević, Marija; Stojmenović, Tamara; Nikolić, Ivan; Đorđević, Brižita

(Canadian Science Publishing, Ottawa, 2016)

TY  - JOUR
AU  - Michalickova, Danica
AU  - Minić, Rajna
AU  - Dikić, Nenad
AU  - Anđelković, Marija
AU  - Kostić-Vučićević, Marija
AU  - Stojmenović, Tamara
AU  - Nikolić, Ivan
AU  - Đorđević, Brižita
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/607
AB  - A randomized, double-blind, placebo-controlled study was conducted to evaluate if Lactobacillus helveticus Lafti L10 (Lallemand Health Solutions, Montreal, Que., Canada) supplementation during 14 weeks in winter can influence the duration, severity, and incidence of upper respiratory tract illness (URTI), as well as to monitor different immune parameters in the population of elite athletes. Before and after the treatment, cardiopulmonary testing and self-rated state of moods evaluation (by Profile of Mood States questionnaire) were performed and blood samples were collected. Thirty-nine elite athletes were randomized either to the placebo (n = 19) or the probiotic (n = 20) group. The probiotic group received L. helveticus Lafti L10, 2 x 10(10) Colony Forming Units. Lafti L10 significantly shortened the URTI episode duration (7.25 +/- 2.90 vs. 10.64 +/- 4.67 days, p = 0.047) and decreased the number of symptoms in the probiotic group (4.92 +/- 1.96 vs. 6.91 +/- 1.22, p = 0.035). Severity and incidence of URTI did not differ between the treatments. There were no significant changes in leukocyte subpopulation abundance, transforming growth factor-beta serum levels, level of interleukin-10 secreted from peptidoglican stimulated peripheral blood mononuclear cells (PBMCs), interferon-gamma level secreted from concanavalin A-stimulated PBMCs or viability/proliferation of PBMCs upon antigen stimulation. Group effect for CD4+/CD8+ ratio was significant (F-[1,F-37] = 6.99, p = 0.020, eta(2) = 0.350); this difference was not significant at baseline, but was evident after 14 weeks (p = 0.02). A significant interaction effect was noted for self-rated sense of vigor (F-[1,F-37] = 11.76, p = 0.009, eta(2) = 0.595). Self-rated sense of vigor increased in the probiotic group (18.5 +/- 4.1 vs. 21.0 +/- 2.6, p = 0.012). Probiotic strain Lafti L10 can be a beneficial nutritional supplement for the reduction of URTI length in elite athletes.
PB  - Canadian Science Publishing, Ottawa
T2  - Applied Physiology Nutrition and Metabolism
T1  - Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial
EP  - 789
IS  - 7
SP  - 782
VL  - 41
DO  - 10.1139/apnm-2015-0541
ER  - 
@article{
author = "Michalickova, Danica and Minić, Rajna and Dikić, Nenad and Anđelković, Marija and Kostić-Vučićević, Marija and Stojmenović, Tamara and Nikolić, Ivan and Đorđević, Brižita",
year = "2016",
abstract = "A randomized, double-blind, placebo-controlled study was conducted to evaluate if Lactobacillus helveticus Lafti L10 (Lallemand Health Solutions, Montreal, Que., Canada) supplementation during 14 weeks in winter can influence the duration, severity, and incidence of upper respiratory tract illness (URTI), as well as to monitor different immune parameters in the population of elite athletes. Before and after the treatment, cardiopulmonary testing and self-rated state of moods evaluation (by Profile of Mood States questionnaire) were performed and blood samples were collected. Thirty-nine elite athletes were randomized either to the placebo (n = 19) or the probiotic (n = 20) group. The probiotic group received L. helveticus Lafti L10, 2 x 10(10) Colony Forming Units. Lafti L10 significantly shortened the URTI episode duration (7.25 +/- 2.90 vs. 10.64 +/- 4.67 days, p = 0.047) and decreased the number of symptoms in the probiotic group (4.92 +/- 1.96 vs. 6.91 +/- 1.22, p = 0.035). Severity and incidence of URTI did not differ between the treatments. There were no significant changes in leukocyte subpopulation abundance, transforming growth factor-beta serum levels, level of interleukin-10 secreted from peptidoglican stimulated peripheral blood mononuclear cells (PBMCs), interferon-gamma level secreted from concanavalin A-stimulated PBMCs or viability/proliferation of PBMCs upon antigen stimulation. Group effect for CD4+/CD8+ ratio was significant (F-[1,F-37] = 6.99, p = 0.020, eta(2) = 0.350); this difference was not significant at baseline, but was evident after 14 weeks (p = 0.02). A significant interaction effect was noted for self-rated sense of vigor (F-[1,F-37] = 11.76, p = 0.009, eta(2) = 0.595). Self-rated sense of vigor increased in the probiotic group (18.5 +/- 4.1 vs. 21.0 +/- 2.6, p = 0.012). Probiotic strain Lafti L10 can be a beneficial nutritional supplement for the reduction of URTI length in elite athletes.",
publisher = "Canadian Science Publishing, Ottawa",
journal = "Applied Physiology Nutrition and Metabolism",
title = "Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial",
pages = "789-782",
number = "7",
volume = "41",
doi = "10.1139/apnm-2015-0541"
}
Michalickova, D., Minić, R., Dikić, N., Anđelković, M., Kostić-Vučićević, M., Stojmenović, T., Nikolić, I.,& Đorđević, B.. (2016). Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial. in Applied Physiology Nutrition and Metabolism
Canadian Science Publishing, Ottawa., 41(7), 782-789.
https://doi.org/10.1139/apnm-2015-0541
Michalickova D, Minić R, Dikić N, Anđelković M, Kostić-Vučićević M, Stojmenović T, Nikolić I, Đorđević B. Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial. in Applied Physiology Nutrition and Metabolism. 2016;41(7):782-789.
doi:10.1139/apnm-2015-0541 .
Michalickova, Danica, Minić, Rajna, Dikić, Nenad, Anđelković, Marija, Kostić-Vučićević, Marija, Stojmenović, Tamara, Nikolić, Ivan, Đorđević, Brižita, "Lactobacillus helveticus Lafti L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized, double-blind, placebo-controlled trial" in Applied Physiology Nutrition and Metabolism, 41, no. 7 (2016):782-789,
https://doi.org/10.1139/apnm-2015-0541 . .
47
36
40

Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime

Ivanović, Nevena; Minić, Rajna; Đuričić, Ivana; Radojević-Skodrić, Sanja; Živković, Irena; Šobajić, Slađana; Đorđević, Brižita

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Ivanović, Nevena
AU  - Minić, Rajna
AU  - Đuričić, Ivana
AU  - Radojević-Skodrić, Sanja
AU  - Živković, Irena
AU  - Šobajić, Slađana
AU  - Đorđević, Brižita
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/472
AB  - Western life style, and high calorie diet in particular is causing major health problems such as insulin resistance, hepatic steatosis and heart disease in the modern age. High fat diet (HFD) induces similar changes in mice, such as increased body weight, hypercholesterolemia and accumulation of triglycerides in the liver. These changes can be ameliorated by the administration of some Lactobacillus species. The focus of this study was to analyze the fatty acid content of liver, heart and brain tissues of mice fed HFD and administered with either Lactobacillus plantarum WCFS1 or Lactobacillus rhamnosus LA68, and to analyze the fatty acid content of these organs after a two months washout period. The fatty acid composition of mouse liver tissue changed significantly due to probiotic administration during a 12 weeks HFD regime and active Lactobacillus administration had a slightly reversing effect toward the standard mouse diet group, but after the washout period these changes disappeared. The fatty acid composition of the heart and brain tissues was significantly changed in the HFD regime but probiotic administration had no significant influence on the fatty acid profile of these two organs. Upon the 8 weeks washout period the only remaining beneficial effect was the significantly lower mouse weight in the supplemented groups compared to the HFD group.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime
EP  - 2848
IS  - 6
SP  - 2840
VL  - 7
DO  - 10.1039/c5fo01432h
UR  - conv_389
ER  - 
@article{
author = "Ivanović, Nevena and Minić, Rajna and Đuričić, Ivana and Radojević-Skodrić, Sanja and Živković, Irena and Šobajić, Slađana and Đorđević, Brižita",
year = "2016",
abstract = "Western life style, and high calorie diet in particular is causing major health problems such as insulin resistance, hepatic steatosis and heart disease in the modern age. High fat diet (HFD) induces similar changes in mice, such as increased body weight, hypercholesterolemia and accumulation of triglycerides in the liver. These changes can be ameliorated by the administration of some Lactobacillus species. The focus of this study was to analyze the fatty acid content of liver, heart and brain tissues of mice fed HFD and administered with either Lactobacillus plantarum WCFS1 or Lactobacillus rhamnosus LA68, and to analyze the fatty acid content of these organs after a two months washout period. The fatty acid composition of mouse liver tissue changed significantly due to probiotic administration during a 12 weeks HFD regime and active Lactobacillus administration had a slightly reversing effect toward the standard mouse diet group, but after the washout period these changes disappeared. The fatty acid composition of the heart and brain tissues was significantly changed in the HFD regime but probiotic administration had no significant influence on the fatty acid profile of these two organs. Upon the 8 weeks washout period the only remaining beneficial effect was the significantly lower mouse weight in the supplemented groups compared to the HFD group.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime",
pages = "2848-2840",
number = "6",
volume = "7",
doi = "10.1039/c5fo01432h",
url = "conv_389"
}
Ivanović, N., Minić, R., Đuričić, I., Radojević-Skodrić, S., Živković, I., Šobajić, S.,& Đorđević, B.. (2016). Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime. in Food & Function
Royal Soc Chemistry, Cambridge., 7(6), 2840-2848.
https://doi.org/10.1039/c5fo01432h
conv_389
Ivanović N, Minić R, Đuričić I, Radojević-Skodrić S, Živković I, Šobajić S, Đorđević B. Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime. in Food & Function. 2016;7(6):2840-2848.
doi:10.1039/c5fo01432h
conv_389 .
Ivanović, Nevena, Minić, Rajna, Đuričić, Ivana, Radojević-Skodrić, Sanja, Živković, Irena, Šobajić, Slađana, Đorđević, Brižita, "Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime" in Food & Function, 7, no. 6 (2016):2840-2848,
https://doi.org/10.1039/c5fo01432h .,
conv_389 .
1
7
7
8

Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes

Đorđević, Brižita; Marinković, Danica; Minić, Rajna; Ivanović, Nevena; Dikić, Nenad; Anđelković, Marija; Živković, Irena; Stanković, Ivan

(Lippincott Williams & Wilkins, Philadelphia, 2016)

TY  - CONF
AU  - Đorđević, Brižita
AU  - Marinković, Danica
AU  - Minić, Rajna
AU  - Ivanović, Nevena
AU  - Dikić, Nenad
AU  - Anđelković, Marija
AU  - Živković, Irena
AU  - Stanković, Ivan
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/452
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Medicine and Science in Sports and Exercise
T1  - Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes
EP  - 246
IS  - 5
SP  - 246
VL  - 48
DO  - 10.1249/01.mss.0000485740.06462.11
UR  - conv_418
ER  - 
@conference{
author = "Đorđević, Brižita and Marinković, Danica and Minić, Rajna and Ivanović, Nevena and Dikić, Nenad and Anđelković, Marija and Živković, Irena and Stanković, Ivan",
year = "2016",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Medicine and Science in Sports and Exercise",
title = "Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes",
pages = "246-246",
number = "5",
volume = "48",
doi = "10.1249/01.mss.0000485740.06462.11",
url = "conv_418"
}
Đorđević, B., Marinković, D., Minić, R., Ivanović, N., Dikić, N., Anđelković, M., Živković, I.,& Stanković, I.. (2016). Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes. in Medicine and Science in Sports and Exercise
Lippincott Williams & Wilkins, Philadelphia., 48(5), 246-246.
https://doi.org/10.1249/01.mss.0000485740.06462.11
conv_418
Đorđević B, Marinković D, Minić R, Ivanović N, Dikić N, Anđelković M, Živković I, Stanković I. Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes. in Medicine and Science in Sports and Exercise. 2016;48(5):246-246.
doi:10.1249/01.mss.0000485740.06462.11
conv_418 .
Đorđević, Brižita, Marinković, Danica, Minić, Rajna, Ivanović, Nevena, Dikić, Nenad, Anđelković, Marija, Živković, Irena, Stanković, Ivan, "Lactobacillus Helveticus L10 Supplementation Modulates Immunity Parameters in Elite Athletes" in Medicine and Science in Sports and Exercise, 48, no. 5 (2016):246-246,
https://doi.org/10.1249/01.mss.0000485740.06462.11 .,
conv_418 .