TY  - DATA
AU  - Stojanović, Marijana
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Kovačević, Ana
AU  - Miljković, Radmila
AU  - Tobias, Joshua
AU  - Schabussova, Irma
AU  - Zlatović, Mario
AU  - Barisani-Asenbauer, Talin
AU  - Wiedermann, Ursula
AU  - Inić-Kanada, Aleksandra
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/643
AB  - Table S1: Characteristics of selected anti-tetanus mAbs [35,36].
PB  - MDPI
T2  - Vaccines
T1  - Supplementary information for the article: Stojanović, M.; Lukić, I.; Marinković, E.; Kovačević, A.; Miljković, R.; Tobias, J.; Schabussova, I.; Zlatović, M.; Barisani-Asenbauer, T.; Wiedermann, U.; Inić-Kanada, A. Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia. Vaccines 2020, 8 (4), 719. https://doi.org/10.3390/vaccines8040719.
IS  - 4
SP  - 719
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_intor_643
ER  - 

@misc{
author = "Stojanović, Marijana and Lukić, Ivana and Marinković, Emilija and Kovačević, Ana and Miljković, Radmila and Tobias, Joshua and Schabussova, Irma and Zlatović, Mario and Barisani-Asenbauer, Talin and Wiedermann, Ursula and Inić-Kanada, Aleksandra",
year = "2020",
abstract = "Table S1: Characteristics of selected anti-tetanus mAbs [35,36].",
publisher = "MDPI",
journal = "Vaccines",
title = "Supplementary information for the article: Stojanović, M.; Lukić, I.; Marinković, E.; Kovačević, A.; Miljković, R.; Tobias, J.; Schabussova, I.; Zlatović, M.; Barisani-Asenbauer, T.; Wiedermann, U.; Inić-Kanada, A. Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia. Vaccines 2020, 8 (4), 719. https://doi.org/10.3390/vaccines8040719.",
number = "4",
pages = "719",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_intor_643"
}

Stojanović, M., Lukić, I., Marinković, E., Kovačević, A., Miljković, R., Tobias, J., Schabussova, I., Zlatović, M., Barisani-Asenbauer, T., Wiedermann, U.,& Inić-Kanada, A.. (2020). Supplementary information for the article: Stojanović, M.; Lukić, I.; Marinković, E.; Kovačević, A.; Miljković, R.; Tobias, J.; Schabussova, I.; Zlatović, M.; Barisani-Asenbauer, T.; Wiedermann, U.; Inić-Kanada, A. Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia. Vaccines 2020, 8 (4), 719. https://doi.org/10.3390/vaccines8040719.. in Vaccines
MDPI., 8(4), 719.
https://hdl.handle.net/21.15107/rcub_intor_643

Stojanović M, Lukić I, Marinković E, Kovačević A, Miljković R, Tobias J, Schabussova I, Zlatović M, Barisani-Asenbauer T, Wiedermann U, Inić-Kanada A. Supplementary information for the article: Stojanović, M.; Lukić, I.; Marinković, E.; Kovačević, A.; Miljković, R.; Tobias, J.; Schabussova, I.; Zlatović, M.; Barisani-Asenbauer, T.; Wiedermann, U.; Inić-Kanada, A. Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia. Vaccines 2020, 8 (4), 719. https://doi.org/10.3390/vaccines8040719.. in Vaccines. 2020;8(4):719.
https://hdl.handle.net/21.15107/rcub_intor_643 .

Stojanović, Marijana, Lukić, Ivana, Marinković, Emilija, Kovačević, Ana, Miljković, Radmila, Tobias, Joshua, Schabussova, Irma, Zlatović, Mario, Barisani-Asenbauer, Talin, Wiedermann, Ursula, Inić-Kanada, Aleksandra, "Supplementary information for the article: Stojanović, M.; Lukić, I.; Marinković, E.; Kovačević, A.; Miljković, R.; Tobias, J.; Schabussova, I.; Zlatović, M.; Barisani-Asenbauer, T.; Wiedermann, U.; Inić-Kanada, A. Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia. Vaccines 2020, 8 (4), 719. https://doi.org/10.3390/vaccines8040719." in Vaccines, 8, no. 4 (2020):719,
https://hdl.handle.net/21.15107/rcub_intor_643 .

TY  - JOUR
AU  - Stojanović, Marijana
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Kovačević, Ana
AU  - Miljković, Radmila
AU  - Tobias, Joshua
AU  - Schabussova, Irma
AU  - Zlatović, Mario
AU  - Barisani-Asenbauer, Talin
AU  - Wiedermann, Ursula
AU  - Inić-Kanada, Aleksandra
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/552
AB  - Vaccines can have heterologous effects on the immune system, i.e., effects other than triggering an immune response against the disease targeted by the vaccine. We investigated whether monoclonal antibodies (mAbs) specific for tetanus could cross-react with Chlamydia and confer heterologous protection against chlamydial infection. The capability of two tetanus-specific mAbs, namely mAb26 and mAb51, to prevent chlamydial infection has been assessed: (i) in vitro, by performing a neutralization assay using human conjunctival epithelial (HCjE) cells infected with Chlamydia trachomatis serovar B, and (ii) in vivo, by using a guinea pig model of Chlamydia caviae-induced inclusion conjunctivitis. The mAb26 has been superior in comparison with mAb51 in the prevention of chlamydial infection in HCjE cells. The mAb26 has conferred approximate to 40% inhibition of the infection, compared to less than 5% inhibition in the presence of the mAb51. In vivo, mAb26 significantly diminished ocular pathology intensity in guinea pigs infected with C. caviae compared to either the mAb51-treated or sham-treated guinea pigs. Our data provide insights that tetanus immunization generates antibodies which induce heterologous chlamydial immunity and promote protection beyond the intended target pathogen.
PB  - MDPI, Basel
T2  - Vaccines
T1  - Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia
IS  - 4
SP  - 719
VL  - 8
DO  - 10.3390/vaccines8040719
ER  - 

@article{
author = "Stojanović, Marijana and Lukić, Ivana and Marinković, Emilija and Kovačević, Ana and Miljković, Radmila and Tobias, Joshua and Schabussova, Irma and Zlatović, Mario and Barisani-Asenbauer, Talin and Wiedermann, Ursula and Inić-Kanada, Aleksandra",
year = "2020",
abstract = "Vaccines can have heterologous effects on the immune system, i.e., effects other than triggering an immune response against the disease targeted by the vaccine. We investigated whether monoclonal antibodies (mAbs) specific for tetanus could cross-react with Chlamydia and confer heterologous protection against chlamydial infection. The capability of two tetanus-specific mAbs, namely mAb26 and mAb51, to prevent chlamydial infection has been assessed: (i) in vitro, by performing a neutralization assay using human conjunctival epithelial (HCjE) cells infected with Chlamydia trachomatis serovar B, and (ii) in vivo, by using a guinea pig model of Chlamydia caviae-induced inclusion conjunctivitis. The mAb26 has been superior in comparison with mAb51 in the prevention of chlamydial infection in HCjE cells. The mAb26 has conferred approximate to 40% inhibition of the infection, compared to less than 5% inhibition in the presence of the mAb51. In vivo, mAb26 significantly diminished ocular pathology intensity in guinea pigs infected with C. caviae compared to either the mAb51-treated or sham-treated guinea pigs. Our data provide insights that tetanus immunization generates antibodies which induce heterologous chlamydial immunity and promote protection beyond the intended target pathogen.",
publisher = "MDPI, Basel",
journal = "Vaccines",
title = "Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia",
number = "4",
pages = "719",
volume = "8",
doi = "10.3390/vaccines8040719"
}

Stojanović, M., Lukić, I., Marinković, E., Kovačević, A., Miljković, R., Tobias, J., Schabussova, I., Zlatović, M., Barisani-Asenbauer, T., Wiedermann, U.,& Inić-Kanada, A.. (2020). Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia. in Vaccines
MDPI, Basel., 8(4), 719.
https://doi.org/10.3390/vaccines8040719

Stojanović M, Lukić I, Marinković E, Kovačević A, Miljković R, Tobias J, Schabussova I, Zlatović M, Barisani-Asenbauer T, Wiedermann U, Inić-Kanada A. Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia. in Vaccines. 2020;8(4):719.
doi:10.3390/vaccines8040719 .

Stojanović, Marijana, Lukić, Ivana, Marinković, Emilija, Kovačević, Ana, Miljković, Radmila, Tobias, Joshua, Schabussova, Irma, Zlatović, Mario, Barisani-Asenbauer, Talin, Wiedermann, Ursula, Inić-Kanada, Aleksandra, "Cross-Reactive Effects of Vaccines: Heterologous Immunity between Tetanus and Chlamydia" in Vaccines, 8, no. 4 (2020):719,
https://doi.org/10.3390/vaccines8040719 . .

TY  - JOUR
AU  - Obradović, Ana
AU  - Matić, Miloš
AU  - Ognjanović, Branka
AU  - Đurđević, Predrag
AU  - Marinković, Emilija
AU  - Ušćumlić, Gordana
AU  - Božić, Bojan
AU  - Božić-Nedeljković, Biljana
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/566
AB  - In this study, a series of synthesized 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives as a potential antiproliferative and antimigratory agents were investigated. The possible antitumor mechanisms of investigated hydantoin derivatives were examined on human breast cancer cell line MDA-MB-231. The cells were treated with different concentrations of compounds (from 0.01 mu M to 100 mu M) during 24 h and 72 h. The proliferation index, nitric oxide production, apoptosis rate, and migration capacity were measured. The cell invasion potential was examined by measuring the level of MMP-9 and COX-2 gene expression. All tested compounds expressed antiproliferative activity and induced dose- and time-dependent increase in the level of nitrites. The investigated molecules significantly decreased cell survival rate, migration capacity and the expression levels of genes included in the process of tumor invasion. Obtained data suggest that the tested hydantoin derivatives express considerable antitumor activity by reducing cell division rate, elevating apoptosis level, and inhibiting the motility and invasiveness of breast cancer cells. The results obtained in this study indicate that investigated compounds express potential as a novel chemotherapeutic agents against breast cancer growth and progression. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
PB  - Elsevier, Amsterdam
T2  - Saudi Pharmaceutical Journal
T1  - Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells
EP  - 254
IS  - 3
SP  - 246
VL  - 28
DO  - 10.1016/j.jsps.2020.01.003
ER  - 

@article{
author = "Obradović, Ana and Matić, Miloš and Ognjanović, Branka and Đurđević, Predrag and Marinković, Emilija and Ušćumlić, Gordana and Božić, Bojan and Božić-Nedeljković, Biljana",
year = "2020",
abstract = "In this study, a series of synthesized 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives as a potential antiproliferative and antimigratory agents were investigated. The possible antitumor mechanisms of investigated hydantoin derivatives were examined on human breast cancer cell line MDA-MB-231. The cells were treated with different concentrations of compounds (from 0.01 mu M to 100 mu M) during 24 h and 72 h. The proliferation index, nitric oxide production, apoptosis rate, and migration capacity were measured. The cell invasion potential was examined by measuring the level of MMP-9 and COX-2 gene expression. All tested compounds expressed antiproliferative activity and induced dose- and time-dependent increase in the level of nitrites. The investigated molecules significantly decreased cell survival rate, migration capacity and the expression levels of genes included in the process of tumor invasion. Obtained data suggest that the tested hydantoin derivatives express considerable antitumor activity by reducing cell division rate, elevating apoptosis level, and inhibiting the motility and invasiveness of breast cancer cells. The results obtained in this study indicate that investigated compounds express potential as a novel chemotherapeutic agents against breast cancer growth and progression. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Pharmaceutical Journal",
title = "Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells",
pages = "254-246",
number = "3",
volume = "28",
doi = "10.1016/j.jsps.2020.01.003"
}

Obradović, A., Matić, M., Ognjanović, B., Đurđević, P., Marinković, E., Ušćumlić, G., Božić, B.,& Božić-Nedeljković, B.. (2020). Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells. in Saudi Pharmaceutical Journal
Elsevier, Amsterdam., 28(3), 246-254.
https://doi.org/10.1016/j.jsps.2020.01.003

Obradović A, Matić M, Ognjanović B, Đurđević P, Marinković E, Ušćumlić G, Božić B, Božić-Nedeljković B. Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells. in Saudi Pharmaceutical Journal. 2020;28(3):246-254.
doi:10.1016/j.jsps.2020.01.003 .

Obradović, Ana, Matić, Miloš, Ognjanović, Branka, Đurđević, Predrag, Marinković, Emilija, Ušćumlić, Gordana, Božić, Bojan, Božić-Nedeljković, Biljana, "Antiproliferative and antimigratory effects of 3-(4-substituted benzyl)-5-isopropyl-5-phenylhydantoin derivatives in human breast cancer cells" in Saudi Pharmaceutical Journal, 28, no. 3 (2020):246-254,
https://doi.org/10.1016/j.jsps.2020.01.003 . .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Filipović, Ana
AU  - Miljković, Radmila
AU  - Barisani-Asenbauer, Talin
AU  - Wiedermann, Ursula
AU  - Stojanović, Marijana
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/528
PB  - Wiley, Hoboken
C3  - European Journal of Immunology
T1  - Beneficial heterologous effects of a tetanus vaccination: the role of molecular mimicry and/or trained immunity
EP  - 1694
SP  - 1693
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_intor_528
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Lukić, Ivana and Marinković, Emilija and Filipović, Ana and Miljković, Radmila and Barisani-Asenbauer, Talin and Wiedermann, Ursula and Stojanović, Marijana",
year = "2019",
publisher = "Wiley, Hoboken",
journal = "European Journal of Immunology",
title = "Beneficial heterologous effects of a tetanus vaccination: the role of molecular mimicry and/or trained immunity",
pages = "1694-1693",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_intor_528"
}

Inić-Kanada, A., Lukić, I., Marinković, E., Filipović, A., Miljković, R., Barisani-Asenbauer, T., Wiedermann, U.,& Stojanović, M.. (2019). Beneficial heterologous effects of a tetanus vaccination: the role of molecular mimicry and/or trained immunity. in European Journal of Immunology
Wiley, Hoboken., 49, 1693-1694.
https://hdl.handle.net/21.15107/rcub_intor_528

Inić-Kanada A, Lukić I, Marinković E, Filipović A, Miljković R, Barisani-Asenbauer T, Wiedermann U, Stojanović M. Beneficial heterologous effects of a tetanus vaccination: the role of molecular mimicry and/or trained immunity. in European Journal of Immunology. 2019;49:1693-1694.
https://hdl.handle.net/21.15107/rcub_intor_528 .

Inić-Kanada, Aleksandra, Lukić, Ivana, Marinković, Emilija, Filipović, Ana, Miljković, Radmila, Barisani-Asenbauer, Talin, Wiedermann, Ursula, Stojanović, Marijana, "Beneficial heterologous effects of a tetanus vaccination: the role of molecular mimicry and/or trained immunity" in European Journal of Immunology, 49 (2019):1693-1694,
https://hdl.handle.net/21.15107/rcub_intor_528 .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Filipović, Ana
AU  - Inić-Kanada, Aleksandra
AU  - Marinković, Emilija
AU  - Miljković, Radmila
AU  - Stojanović, Marijana
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/510
AB  - Oligoclonal combinations of several monoclonal antibodies (MAbs) are being considered for the treatment of various infectious pathologies. These combinations are less sensitive to antigen structural changes than individual MAbs; at the same time, their characteristics can be more efficiently controlled than those of polyclonal antibodies. The main goal of this study was to evaluate the binding characteristics of six biclonal equimolar preparations (BEP) of tetanus toxin (TeNT)-specific MAbs and to investigate how the MAb combination influences the BEPs' protective capacity. We show that a combination of TeNT-specific MAbs, which not only bind TeNT but also exert positive cooperative effects, results in a BEP with superior binding characteristics and protective capacity, when compared with the individual component MAbs. Furthermore, we show that a MAb with only partial protective capacity but positive effects on the binding of the other BEP component can be used as a valuable constituent of the BEP. (C) 2018 Elsevier Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Vaccine
T1  - Cooperative binding of anti-tetanus toxin monoclonal antibodies: Implications for designing an efficient biclonal preparation to prevent tetanus toxin intoxication
EP  - 3771
IS  - 26
SP  - 3764
VL  - 36
DO  - 10.1016/j.vaccine.2018.05.058
ER  - 

@article{
author = "Lukić, Ivana and Filipović, Ana and Inić-Kanada, Aleksandra and Marinković, Emilija and Miljković, Radmila and Stojanović, Marijana",
year = "2018",
abstract = "Oligoclonal combinations of several monoclonal antibodies (MAbs) are being considered for the treatment of various infectious pathologies. These combinations are less sensitive to antigen structural changes than individual MAbs; at the same time, their characteristics can be more efficiently controlled than those of polyclonal antibodies. The main goal of this study was to evaluate the binding characteristics of six biclonal equimolar preparations (BEP) of tetanus toxin (TeNT)-specific MAbs and to investigate how the MAb combination influences the BEPs' protective capacity. We show that a combination of TeNT-specific MAbs, which not only bind TeNT but also exert positive cooperative effects, results in a BEP with superior binding characteristics and protective capacity, when compared with the individual component MAbs. Furthermore, we show that a MAb with only partial protective capacity but positive effects on the binding of the other BEP component can be used as a valuable constituent of the BEP. (C) 2018 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Vaccine",
title = "Cooperative binding of anti-tetanus toxin monoclonal antibodies: Implications for designing an efficient biclonal preparation to prevent tetanus toxin intoxication",
pages = "3771-3764",
number = "26",
volume = "36",
doi = "10.1016/j.vaccine.2018.05.058"
}

Lukić, I., Filipović, A., Inić-Kanada, A., Marinković, E., Miljković, R.,& Stojanović, M.. (2018). Cooperative binding of anti-tetanus toxin monoclonal antibodies: Implications for designing an efficient biclonal preparation to prevent tetanus toxin intoxication. in Vaccine
Elsevier Sci Ltd, Oxford., 36(26), 3764-3771.
https://doi.org/10.1016/j.vaccine.2018.05.058

Lukić I, Filipović A, Inić-Kanada A, Marinković E, Miljković R, Stojanović M. Cooperative binding of anti-tetanus toxin monoclonal antibodies: Implications for designing an efficient biclonal preparation to prevent tetanus toxin intoxication. in Vaccine. 2018;36(26):3764-3771.
doi:10.1016/j.vaccine.2018.05.058 .

Lukić, Ivana, Filipović, Ana, Inić-Kanada, Aleksandra, Marinković, Emilija, Miljković, Radmila, Stojanović, Marijana, "Cooperative binding of anti-tetanus toxin monoclonal antibodies: Implications for designing an efficient biclonal preparation to prevent tetanus toxin intoxication" in Vaccine, 36, no. 26 (2018):3764-3771,
https://doi.org/10.1016/j.vaccine.2018.05.058 . .

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stein, Elisabeth
AU  - Stojanović, Marijana
AU  - Schuerer, Nadine
AU  - Ghasemian, Ehsan
AU  - Filipović, Ana
AU  - Marinković, Emilija
AU  - Kosanović, Dejana
AU  - Barisani-Asenbauer, Talin
PY  - 2018
UR  - http://intor.torlakinstitut.com/handle/123456789/502
AB  - Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.
PB  - Springer, Dordrecht
T2  - Journal of Applied Phycology
T1  - Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo
EP  - 2610
IS  - 4
SP  - 2601
VL  - 30
DO  - 10.1007/s10811-018-1435-0
ER  - 

@article{
author = "Inić-Kanada, Aleksandra and Stein, Elisabeth and Stojanović, Marijana and Schuerer, Nadine and Ghasemian, Ehsan and Filipović, Ana and Marinković, Emilija and Kosanović, Dejana and Barisani-Asenbauer, Talin",
year = "2018",
abstract = "Ocular chlamydial infections with the ocular serovars A, B, Ba, and C of Chlamydia trachomatis represent the world's leading cause of infectious blindness. Carrageenans are naturally occurring, sulfated polysaccharides generally considered safe for food and topical applications. Carrageenans can inhibit infection caused by a variety of viruses and bacteria. To investigate whether iota-carrageenan (I-C) isolated from the red alga Chondrus crispus could prevent ocular chlamydial infection, we assessed if targeted treatment of the conjunctival mucosa with I-C affects chlamydial attachment, entry, and replication in the host cell. Immortalized human conjunctival epithelial cells were treated with I-C prior to C. trachomatis infection and analyzed by flow cytometry and immunofluorescence microscopy. In vivo effects were evaluated in an ocular guinea pig inclusion conjunctivitis model. Ocular pathology was graded daily, and chlamydial clearance was investigated. Our study showed that I-C reduces the infectivity of C. trachomatis in vitro. In vivo results showed a slight reduced ocular pathology and significantly less shedding of infectious elementary bodies by infected animals. Our results indicate that I-C could be a promising agent to reduce the transmission of ocular chlamydial infection and opens perspectives to develop prophylactic approaches to block C. trachomatis entry into the host cell.",
publisher = "Springer, Dordrecht",
journal = "Journal of Applied Phycology",
title = "Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo",
pages = "2610-2601",
number = "4",
volume = "30",
doi = "10.1007/s10811-018-1435-0"
}

Inić-Kanada, A., Stein, E., Stojanović, M., Schuerer, N., Ghasemian, E., Filipović, A., Marinković, E., Kosanović, D.,& Barisani-Asenbauer, T.. (2018). Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo. in Journal of Applied Phycology
Springer, Dordrecht., 30(4), 2601-2610.
https://doi.org/10.1007/s10811-018-1435-0

Inić-Kanada A, Stein E, Stojanović M, Schuerer N, Ghasemian E, Filipović A, Marinković E, Kosanović D, Barisani-Asenbauer T. Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo. in Journal of Applied Phycology. 2018;30(4):2601-2610.
doi:10.1007/s10811-018-1435-0 .

Inić-Kanada, Aleksandra, Stein, Elisabeth, Stojanović, Marijana, Schuerer, Nadine, Ghasemian, Ehsan, Filipović, Ana, Marinković, Emilija, Kosanović, Dejana, Barisani-Asenbauer, Talin, "Effects of iota-carrageenan on ocular Chlamydia trachomatis infection in vitro and in vivo" in Journal of Applied Phycology, 30, no. 4 (2018):2601-2610,
https://doi.org/10.1007/s10811-018-1435-0 . .

TY  - JOUR
AU  - Marinković, Emilija
AU  - Đokić, Radmila
AU  - Lukić, Ivana
AU  - Filipović, Ana
AU  - Inić-Kanada, Aleksandra
AU  - Kosanović, Dejana
AU  - Gavrović-Jankulović, Marija
AU  - Stojanović, Marijana
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/493
AB  - We demonstrated that a recombinant banana lectin (rBanLec), which structural characteristics and physiological impacts highly resemble those reported for its natural counterparts, binds murine peritoneal macrophages and specifically modulates their functional characteristics. By using rBanLec in concentrations ranging from 1 mu g to 10 mu g to stimulate resident (RMs) and thioglycollate-elicited (TGMs) peritoneal macrophages from BALB/c and C57BL/6 mice, we have shown that effects of rBanLec stimulation depend on its concentration but also on the functional status of macrophages and their genetic background. rBanLec, in a positive dose-dependent manner, promotes the proliferation of TGMs from both BALB/c and C57BL/6 mice, while its mitogenic influence on RMs is significantly lower (BALB/c mice) or not detectable (C57BL/6 mice). In all peritoneal macrophages, irrespective of their type and genetic background, rBanLec, in a positive dose dependent manner, enhances the secretion of IL-10. rBanLec stimulation of RMs from both BALB/c and C57BL/6 resulted in a positive dose-dependent promotion of proinflammatory phenotype (enhancement of NO production and IL-12 and TNF alpha secretion, reduction of arginase activity). Positive dose-dependent skewing toward proinflammatory phenotype was also observed in TGMs from C57BL/6 mice. However, the enhancement of rBanLec stimulation promotes skewing of TGMs from BALB/c mice towards anti-inflammatory profile (reduction of NO production and IL-12 secretion, enhancement of arginase activity and TGF alpha and IL-4 secretion). Moreover, we established that rBanLec binds oligosaccharide structures of TLR2 and CD14 and that blocking of signaling via these receptors significantly impairs the production of TNFa and NO in BALB/c macrophages. Since the outcome of rBanLec stimulation depends on rBanLec concentration as well as on the functional characteristics of its target cells and their genetic background, further studies are needed to investigate its effects under physiological and specific pathological conditions.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin
IS  - 2
VL  - 12
DO  - 10.1371/journal.pone.0172469
ER  - 

@article{
author = "Marinković, Emilija and Đokić, Radmila and Lukić, Ivana and Filipović, Ana and Inić-Kanada, Aleksandra and Kosanović, Dejana and Gavrović-Jankulović, Marija and Stojanović, Marijana",
year = "2017",
abstract = "We demonstrated that a recombinant banana lectin (rBanLec), which structural characteristics and physiological impacts highly resemble those reported for its natural counterparts, binds murine peritoneal macrophages and specifically modulates their functional characteristics. By using rBanLec in concentrations ranging from 1 mu g to 10 mu g to stimulate resident (RMs) and thioglycollate-elicited (TGMs) peritoneal macrophages from BALB/c and C57BL/6 mice, we have shown that effects of rBanLec stimulation depend on its concentration but also on the functional status of macrophages and their genetic background. rBanLec, in a positive dose-dependent manner, promotes the proliferation of TGMs from both BALB/c and C57BL/6 mice, while its mitogenic influence on RMs is significantly lower (BALB/c mice) or not detectable (C57BL/6 mice). In all peritoneal macrophages, irrespective of their type and genetic background, rBanLec, in a positive dose dependent manner, enhances the secretion of IL-10. rBanLec stimulation of RMs from both BALB/c and C57BL/6 resulted in a positive dose-dependent promotion of proinflammatory phenotype (enhancement of NO production and IL-12 and TNF alpha secretion, reduction of arginase activity). Positive dose-dependent skewing toward proinflammatory phenotype was also observed in TGMs from C57BL/6 mice. However, the enhancement of rBanLec stimulation promotes skewing of TGMs from BALB/c mice towards anti-inflammatory profile (reduction of NO production and IL-12 secretion, enhancement of arginase activity and TGF alpha and IL-4 secretion). Moreover, we established that rBanLec binds oligosaccharide structures of TLR2 and CD14 and that blocking of signaling via these receptors significantly impairs the production of TNFa and NO in BALB/c macrophages. Since the outcome of rBanLec stimulation depends on rBanLec concentration as well as on the functional characteristics of its target cells and their genetic background, further studies are needed to investigate its effects under physiological and specific pathological conditions.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin",
number = "2",
volume = "12",
doi = "10.1371/journal.pone.0172469"
}

Marinković, E., Đokić, R., Lukić, I., Filipović, A., Inić-Kanada, A., Kosanović, D., Gavrović-Jankulović, M.,& Stojanović, M.. (2017). Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin. in PLoS One
Public Library Science, San Francisco., 12(2).
https://doi.org/10.1371/journal.pone.0172469

Marinković E, Đokić R, Lukić I, Filipović A, Inić-Kanada A, Kosanović D, Gavrović-Jankulović M, Stojanović M. Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin. in PLoS One. 2017;12(2).
doi:10.1371/journal.pone.0172469 .

Marinković, Emilija, Đokić, Radmila, Lukić, Ivana, Filipović, Ana, Inić-Kanada, Aleksandra, Kosanović, Dejana, Gavrović-Jankulović, Marija, Stojanović, Marijana, "Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin" in PLoS One, 12, no. 2 (2017),
https://doi.org/10.1371/journal.pone.0172469 . .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Lukić, Ivana
AU  - Stojanović, Marijana
AU  - Stein, Elisabeth
AU  - Marinković, Emilija
AU  - Filipović, Ana
AU  - Đokić, Radmila
AU  - Kosanović, Dejana
AU  - Schuerer, Nadine
AU  - Ghasemian, Ehsan
AU  - Barisani-Asenbauer, Talin
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/486
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.
IS  - 8
VL  - 58
UR  - https://hdl.handle.net/21.15107/rcub_intor_486
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Lukić, Ivana and Stojanović, Marijana and Stein, Elisabeth and Marinković, Emilija and Filipović, Ana and Đokić, Radmila and Kosanović, Dejana and Schuerer, Nadine and Ghasemian, Ehsan and Barisani-Asenbauer, Talin",
year = "2017",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.",
number = "8",
volume = "58",
url = "https://hdl.handle.net/21.15107/rcub_intor_486"
}

Inić-Kanada, A., Lukić, I., Stojanović, M., Stein, E., Marinković, E., Filipović, A., Đokić, R., Kosanović, D., Schuerer, N., Ghasemian, E.,& Barisani-Asenbauer, T.. (2017). Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 58(8).
https://hdl.handle.net/21.15107/rcub_intor_486

Inić-Kanada A, Lukić I, Stojanović M, Stein E, Marinković E, Filipović A, Đokić R, Kosanović D, Schuerer N, Ghasemian E, Barisani-Asenbauer T. Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection.. in Investigative Ophthalmology & Visual Science. 2017;58(8).
https://hdl.handle.net/21.15107/rcub_intor_486 .

Inić-Kanada, Aleksandra, Lukić, Ivana, Stojanović, Marijana, Stein, Elisabeth, Marinković, Emilija, Filipović, Ana, Đokić, Radmila, Kosanović, Dejana, Schuerer, Nadine, Ghasemian, Ehsan, Barisani-Asenbauer, Talin, "Tetanus vaccination related to the decline of trachoma in the Western World? Anti-tetanus antibodies confer partial protection against ocular chlamydial infection." in Investigative Ophthalmology & Visual Science, 58, no. 8 (2017),
https://hdl.handle.net/21.15107/rcub_intor_486 .

TY  - CONF
AU  - Marinković, Emilija
AU  - Đokić, Radmila
AU  - Filipović, Ana
AU  - Lukić, Ivana
AU  - Kosanović, Dejana
AU  - Inić-Kanada, Aleksandra
AU  - Gavrović-Jankulović, Marija
AU  - Stojanović, Marijana
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/478
PB  - Wiley, Hoboken
C3  - FEBS Journal
T1  - Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice
EP  - 125
SP  - 124
VL  - 284
UR  - https://hdl.handle.net/21.15107/rcub_intor_478
ER  - 

@conference{
author = "Marinković, Emilija and Đokić, Radmila and Filipović, Ana and Lukić, Ivana and Kosanović, Dejana and Inić-Kanada, Aleksandra and Gavrović-Jankulović, Marija and Stojanović, Marijana",
year = "2017",
publisher = "Wiley, Hoboken",
journal = "FEBS Journal",
title = "Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice",
pages = "125-124",
volume = "284",
url = "https://hdl.handle.net/21.15107/rcub_intor_478"
}

Marinković, E., Đokić, R., Filipović, A., Lukić, I., Kosanović, D., Inić-Kanada, A., Gavrović-Jankulović, M.,& Stojanović, M.. (2017). Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice. in FEBS Journal
Wiley, Hoboken., 284, 124-125.
https://hdl.handle.net/21.15107/rcub_intor_478

Marinković E, Đokić R, Filipović A, Lukić I, Kosanović D, Inić-Kanada A, Gavrović-Jankulović M, Stojanović M. Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice. in FEBS Journal. 2017;284:124-125.
https://hdl.handle.net/21.15107/rcub_intor_478 .

Marinković, Emilija, Đokić, Radmila, Filipović, Ana, Lukić, Ivana, Kosanović, Dejana, Inić-Kanada, Aleksandra, Gavrović-Jankulović, Marija, Stojanović, Marijana, "Prophylactic effect of recombinant banana lectin on TNBS-induced colitis in BALB/c mice" in FEBS Journal, 284 (2017):124-125,
https://hdl.handle.net/21.15107/rcub_intor_478 .

TY  - JOUR
AU  - Filipović, Ana
AU  - Ghasemian, Ehsan
AU  - Inić-Kanada, Aleksandra
AU  - Lukić, Ivana
AU  - Stein, Elisabeth
AU  - Marinković, Emilija
AU  - Đokić, Radmila
AU  - Kosanović, Dejana
AU  - Schuerer, Nadine
AU  - Chalabi, Hadeel
AU  - Belij-Rammerstorfer, Sandra
AU  - Stojanović, Marijana
AU  - Barisani-Asenbauer, Talin
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/477
AB  - Following infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1x10(2), 1x10(4), and 1x10(6) inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4(+) and CD8(+) cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4(+) and CD8(+) cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4(+) and CD8(+) cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1x10(4), and 1x10(6) IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection
IS  - 7
VL  - 12
DO  - 10.1371/journal.pone.0180551
ER  - 

@article{
author = "Filipović, Ana and Ghasemian, Ehsan and Inić-Kanada, Aleksandra and Lukić, Ivana and Stein, Elisabeth and Marinković, Emilija and Đokić, Radmila and Kosanović, Dejana and Schuerer, Nadine and Chalabi, Hadeel and Belij-Rammerstorfer, Sandra and Stojanović, Marijana and Barisani-Asenbauer, Talin",
year = "2017",
abstract = "Following infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1x10(2), 1x10(4), and 1x10(6) inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4(+) and CD8(+) cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4(+) and CD8(+) cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4(+) and CD8(+) cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1x10(4), and 1x10(6) IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection",
number = "7",
volume = "12",
doi = "10.1371/journal.pone.0180551"
}

Filipović, A., Ghasemian, E., Inić-Kanada, A., Lukić, I., Stein, E., Marinković, E., Đokić, R., Kosanović, D., Schuerer, N., Chalabi, H., Belij-Rammerstorfer, S., Stojanović, M.,& Barisani-Asenbauer, T.. (2017). The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection. in PLoS One
Public Library Science, San Francisco., 12(7).
https://doi.org/10.1371/journal.pone.0180551

Filipović A, Ghasemian E, Inić-Kanada A, Lukić I, Stein E, Marinković E, Đokić R, Kosanović D, Schuerer N, Chalabi H, Belij-Rammerstorfer S, Stojanović M, Barisani-Asenbauer T. The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection. in PLoS One. 2017;12(7).
doi:10.1371/journal.pone.0180551 .

Filipović, Ana, Ghasemian, Ehsan, Inić-Kanada, Aleksandra, Lukić, Ivana, Stein, Elisabeth, Marinković, Emilija, Đokić, Radmila, Kosanović, Dejana, Schuerer, Nadine, Chalabi, Hadeel, Belij-Rammerstorfer, Sandra, Stojanović, Marijana, Barisani-Asenbauer, Talin, "The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection" in PLoS One, 12, no. 7 (2017),
https://doi.org/10.1371/journal.pone.0180551 . .

TY  - THES
AU  - Marinković, Emilija
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5511
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:16902/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025177010
UR  - http://nardus.mpn.gov.rs/handle/123456789/9052
UR  - http://intor.torlakinstitut.com/handle/123456789/575
AB  - Banana lectin (BanLec) is primarily isolated from the fruit of banana (Musa paradisiac). It is glucose / mannose-specific lectin that belongs to the jackalin superfamily of lectins. There are several naturally occurring BanLec isoforms. Nowadays BanLec can also be produced by recombinant technology (rBanLec). rBanLec possesses structural and functional characteristics that highly resemble those reported for its natural counterparts. As most plant lectins, (r)BanLec is resistant to denaturation/proteolytic cleavage in the gastrointestinal tract. It has been reported that rBanLec attaches specifically to the mucosal surface of small intestine and passes into subepithelial compartment. (r)BanLec has been recognized as a potential immunomodulator. It has been shown that (r)BanLec modulates functional characteristics of lymphocytes but the effects of (r)BanLec stimulation in other immune cells are not yet elucidated. The aim of this study was to investigate immunomodulatory / immunostimulatory activity of rBanLec in the colon of BALB/c mice. This aim was accomplished through evaluation of the impact of rBanLec stimulation on 1) functional characteristics of antigen presenting cells (APC) isolated from BALB/c mice 2) local immune system in the large intestine of healthy BALB/c mice, and 3) the course of TNBS-induced experimental colitis in BALB/c mice. Peritoneal macrophages and spleen dendritic cells (DC) isolated from BALB/c mice were used in vitro for the evaluation of rBanLec influence on functional characteristics of APC. Generally, it is shown that rBanLec in a dose-dependent manner modulated the functional characteristics of APCs. By using resident (RMs) and thioglycollate-elicited (TGMs) peritoneal macrophages, it has been shown that effects of rBanLec stimulation depend on its concentration but also on the functional status of macrophages. Obtained results have clearly shown that rBanLec, in a positive dosedependent manner, promotes pro-inflammatory phenotype with BALB/c RMs (enhancement of NO and IL-12, reduction of arginase activity and IL-4 secretion) while, in the same manner, it tends to skew BALB/c TGMs towards anti-inflammatory profile (reduction of NO and IL-12 production, enhancement of arginase activity and IL-4 secretion). The dose-dependent changes in production of TGF-β by peritoneal macrophages also depended on their functional status: rBanLec stimulated the production of TGF-β by RMs in a negative dose-dependent manner, while in TGMs production of TGF-β positively correlated to the rBanLec concentration. The activity of myeloperoxidase (MPO) and productions of TNF-α and IL-10 were enhanced upon rBanLec stimulation in a positive dose-dependent manner with both RMs and TGMs. Further, it was shown that interactions of rBanLec with TLR2 and TLR4 / CD14 are important for initiations of the production of pro-inflammatory mediators by peritoneal macrophages irrespective to their functional status. rBanLec also stimulated in a specific dose-dependent manner the secretion of effector cytokines IFN-γ and IL-4 (negative dose dependent manner) and regulatory cytokine IL-10 (positive dose-dependent manner) by spleen DCs...
AB  - Lektin banane (BanLec) pripada podgrupi lektina koji vezuju glukozu ili manoza- u okviru familije lektina sličnih žakalinu. U plodu banane, koji predstavlja prirodni izvor BanLec-a, javlja se u više izoformi, a može se proizvesti i rekombinantnom DNK tehnologijom. Rekombinantna izoforma BanLec-a (rBanLec) je po svojim strukturnim karakteristikama i specifičnosti vrlo slična prirodnim izoformama. Kao i većina biljnih lektina, (r)BanLec ne podleže brzo denaturaciji / razgradnji u uslovima digestivnog trakta, a pokazano je da se vezuje za epitel tankog creva i postepeno prolazi u subepitelni prostor. Danas je poznato da BanLec, nezavisno od izoforme, ima imunomodulatorni potencijal. Dosadašnja istraživanja su se dominantno bavila uticajem (r)BanLec-a na funkcionalne karakteristike limfocita i pokazano je da stimulacije određene ćelijske populacije prirodnim i rekombinantnom izoformom kvalitativno imaju isti ishod. Uticaj (r)BanLec-a na funkcionalne karakteristike drugih ćelija imunskog sistema nije detaljno analiziran. Cilj ove doktorske teze je da se kroz ispitivanja (1) modulatornog dejstva rBanLeca na funkcionalne karakteristike antigen-prezentujućih ćelija (APĆ) BALB/c miša, (2) imunomodulatornog dejstva rBanLec-a u debelom crevu BALB/c miša u fiziološkim uslovima, i (3) efekata profilaktičke i terapijske primene rBanLec-a u modelu TNBS-om indukovanog kolitisa kod BALB/c miša, dobije uvid u imunomodulatorni efekat rBanLec-a na imunski sistem u mukozi debelog creva miševa BALB/c soja pod specifičnim uslovima. Koristeći peritonealne makrofage i dendritske ćelije slezine (DĆ) kao in vitro model sisteme, pokazano je da rBanLec dozno-zavisno moduliše funkcionalne karakteristike APĆ BALB/c miševa. Analiza uticaja rBanLec stimulacije na karakteristike rezidentnih (RM) i tioglikolatom-indukovanh (TGM) peritonealnh makrofaga, pokazala je da ishod stimulacije određene ćelijske populacije nije jednoznačan već zavisi od njihovog funkcionalnog stanja. rBanLec na pozitivan dozno-zavisan način pospešuje proinflamatorni kapacitet RM (povećanje produkcije NO i IL-12 i smanjenje aktivnosti arginaze i sekrecije IL-4), a na isti način smanjuje proinflamatorni kapacitet TGM (smanjenje produkcije NO i IL-12 i povećanje aktivnosti arginaze i sekrecije IL-4). Uticaj rBanLec-a na produkciju TGF-β kod peritonealnih makrofaga takođe zavisi od funkcionalnog stanja makrofaga (kod RM, rBanLec negativno dozno-zavisan, a kod TGM pozitivno dozno-zavisno utiče na produkciju TGF-β). I kod RM i kod TGM, rBanLec na pozitivan dozno-zavisan način podstiče aktivnost mijeloperoksidaze (MPO), produkciju TNF-α i IL-10. Za stimulaciju produkcije proinflamatornih medijatora, nezavisno od funkcionalnog stanja makrofaga, značajno je vezivanje rBanLec-a za TLR2 i TLR4 / CD14. rBanLec utiče na sekreciju efektorskih citokina IFN-γ i IL-4 (negativno dozno-zavisno) i regulatornog citokina IL-10 (pozitivno dozno-zavisno) od strane DĆ slezine. rBanLec TLR2-posredovana stimulacija DĆ slezine nije ključna za promene u ekspresiji Ifn-γ i Il-4...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Immunomodulatory activity of recombinant banana lectin isoform in large intestine of BALB/c mice under physiological and pathological conditions
T1  - Imunomodulatorna aktivnost rekombinanatne izoforme lektina iz banane u fiziološkim i patološkim uslovima u debelom crevu miševa BALB/c soja
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9052
ER  - 

@phdthesis{
author = "Marinković, Emilija",
year = "2017",
abstract = "Banana lectin (BanLec) is primarily isolated from the fruit of banana (Musa paradisiac). It is glucose / mannose-specific lectin that belongs to the jackalin superfamily of lectins. There are several naturally occurring BanLec isoforms. Nowadays BanLec can also be produced by recombinant technology (rBanLec). rBanLec possesses structural and functional characteristics that highly resemble those reported for its natural counterparts. As most plant lectins, (r)BanLec is resistant to denaturation/proteolytic cleavage in the gastrointestinal tract. It has been reported that rBanLec attaches specifically to the mucosal surface of small intestine and passes into subepithelial compartment. (r)BanLec has been recognized as a potential immunomodulator. It has been shown that (r)BanLec modulates functional characteristics of lymphocytes but the effects of (r)BanLec stimulation in other immune cells are not yet elucidated. The aim of this study was to investigate immunomodulatory / immunostimulatory activity of rBanLec in the colon of BALB/c mice. This aim was accomplished through evaluation of the impact of rBanLec stimulation on 1) functional characteristics of antigen presenting cells (APC) isolated from BALB/c mice 2) local immune system in the large intestine of healthy BALB/c mice, and 3) the course of TNBS-induced experimental colitis in BALB/c mice. Peritoneal macrophages and spleen dendritic cells (DC) isolated from BALB/c mice were used in vitro for the evaluation of rBanLec influence on functional characteristics of APC. Generally, it is shown that rBanLec in a dose-dependent manner modulated the functional characteristics of APCs. By using resident (RMs) and thioglycollate-elicited (TGMs) peritoneal macrophages, it has been shown that effects of rBanLec stimulation depend on its concentration but also on the functional status of macrophages. Obtained results have clearly shown that rBanLec, in a positive dosedependent manner, promotes pro-inflammatory phenotype with BALB/c RMs (enhancement of NO and IL-12, reduction of arginase activity and IL-4 secretion) while, in the same manner, it tends to skew BALB/c TGMs towards anti-inflammatory profile (reduction of NO and IL-12 production, enhancement of arginase activity and IL-4 secretion). The dose-dependent changes in production of TGF-β by peritoneal macrophages also depended on their functional status: rBanLec stimulated the production of TGF-β by RMs in a negative dose-dependent manner, while in TGMs production of TGF-β positively correlated to the rBanLec concentration. The activity of myeloperoxidase (MPO) and productions of TNF-α and IL-10 were enhanced upon rBanLec stimulation in a positive dose-dependent manner with both RMs and TGMs. Further, it was shown that interactions of rBanLec with TLR2 and TLR4 / CD14 are important for initiations of the production of pro-inflammatory mediators by peritoneal macrophages irrespective to their functional status. rBanLec also stimulated in a specific dose-dependent manner the secretion of effector cytokines IFN-γ and IL-4 (negative dose dependent manner) and regulatory cytokine IL-10 (positive dose-dependent manner) by spleen DCs..., Lektin banane (BanLec) pripada podgrupi lektina koji vezuju glukozu ili manoza- u okviru familije lektina sličnih žakalinu. U plodu banane, koji predstavlja prirodni izvor BanLec-a, javlja se u više izoformi, a može se proizvesti i rekombinantnom DNK tehnologijom. Rekombinantna izoforma BanLec-a (rBanLec) je po svojim strukturnim karakteristikama i specifičnosti vrlo slična prirodnim izoformama. Kao i većina biljnih lektina, (r)BanLec ne podleže brzo denaturaciji / razgradnji u uslovima digestivnog trakta, a pokazano je da se vezuje za epitel tankog creva i postepeno prolazi u subepitelni prostor. Danas je poznato da BanLec, nezavisno od izoforme, ima imunomodulatorni potencijal. Dosadašnja istraživanja su se dominantno bavila uticajem (r)BanLec-a na funkcionalne karakteristike limfocita i pokazano je da stimulacije određene ćelijske populacije prirodnim i rekombinantnom izoformom kvalitativno imaju isti ishod. Uticaj (r)BanLec-a na funkcionalne karakteristike drugih ćelija imunskog sistema nije detaljno analiziran. Cilj ove doktorske teze je da se kroz ispitivanja (1) modulatornog dejstva rBanLeca na funkcionalne karakteristike antigen-prezentujućih ćelija (APĆ) BALB/c miša, (2) imunomodulatornog dejstva rBanLec-a u debelom crevu BALB/c miša u fiziološkim uslovima, i (3) efekata profilaktičke i terapijske primene rBanLec-a u modelu TNBS-om indukovanog kolitisa kod BALB/c miša, dobije uvid u imunomodulatorni efekat rBanLec-a na imunski sistem u mukozi debelog creva miševa BALB/c soja pod specifičnim uslovima. Koristeći peritonealne makrofage i dendritske ćelije slezine (DĆ) kao in vitro model sisteme, pokazano je da rBanLec dozno-zavisno moduliše funkcionalne karakteristike APĆ BALB/c miševa. Analiza uticaja rBanLec stimulacije na karakteristike rezidentnih (RM) i tioglikolatom-indukovanh (TGM) peritonealnh makrofaga, pokazala je da ishod stimulacije određene ćelijske populacije nije jednoznačan već zavisi od njihovog funkcionalnog stanja. rBanLec na pozitivan dozno-zavisan način pospešuje proinflamatorni kapacitet RM (povećanje produkcije NO i IL-12 i smanjenje aktivnosti arginaze i sekrecije IL-4), a na isti način smanjuje proinflamatorni kapacitet TGM (smanjenje produkcije NO i IL-12 i povećanje aktivnosti arginaze i sekrecije IL-4). Uticaj rBanLec-a na produkciju TGF-β kod peritonealnih makrofaga takođe zavisi od funkcionalnog stanja makrofaga (kod RM, rBanLec negativno dozno-zavisan, a kod TGM pozitivno dozno-zavisno utiče na produkciju TGF-β). I kod RM i kod TGM, rBanLec na pozitivan dozno-zavisan način podstiče aktivnost mijeloperoksidaze (MPO), produkciju TNF-α i IL-10. Za stimulaciju produkcije proinflamatornih medijatora, nezavisno od funkcionalnog stanja makrofaga, značajno je vezivanje rBanLec-a za TLR2 i TLR4 / CD14. rBanLec utiče na sekreciju efektorskih citokina IFN-γ i IL-4 (negativno dozno-zavisno) i regulatornog citokina IL-10 (pozitivno dozno-zavisno) od strane DĆ slezine. rBanLec TLR2-posredovana stimulacija DĆ slezine nije ključna za promene u ekspresiji Ifn-γ i Il-4...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Immunomodulatory activity of recombinant banana lectin isoform in large intestine of BALB/c mice under physiological and pathological conditions, Imunomodulatorna aktivnost rekombinanatne izoforme lektina iz banane u fiziološkim i patološkim uslovima u debelom crevu miševa BALB/c soja",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9052"
}

Marinković, E.. (2017). Immunomodulatory activity of recombinant banana lectin isoform in large intestine of BALB/c mice under physiological and pathological conditions. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_9052

Marinković E. Immunomodulatory activity of recombinant banana lectin isoform in large intestine of BALB/c mice under physiological and pathological conditions. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_9052 .

Marinković, Emilija, "Immunomodulatory activity of recombinant banana lectin isoform in large intestine of BALB/c mice under physiological and pathological conditions" (2017),
https://hdl.handle.net/21.15107/rcub_nardus_9052 .

TY  - JOUR
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Kosanović, Dejana
AU  - Inić-Kanada, Aleksandra
AU  - Gavrović-Jankulović, Marija
AU  - Stojanović, Marijana
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/471
AB  - Recombinant banana lectin isoform (rBanLec) attaches specifically to the mucosal surface, crosses the epithelial barrier and then directly affects the immune response in mouse colon. Structural characteristics, specificity and physiological impacts of rBanLec reported until now highly resemble those of its natural counterpart. Here, we demonstrated that a dose dependent stimulation of the colon with rBanLec skewed the immune response towards Th1/Th17 direction and this effect was counterbalanced by the rise in IL-10 production. Qualitative and quantitative characteristics of the established cytokine network were dependent on the applied rBanLec concentration. In addition, rBanLec enhanced local NO production and myeloperoxidase activity and promoted an increase in local IgA and IgG production. Stimulation with rBanLec can be beneficial in prevention of pathologies raised due to inappropriate cell-mediated immune response as well as in prevention of the pathogen invasion via the colon. (C) 2015 Elsevier Ltd. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Journal of Functional Foods
T1  - Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon
EP  - 78
SP  - 68
VL  - 20
DO  - 10.1016/j.jff.2015.10.019
ER  - 

@article{
author = "Marinković, Emilija and Lukić, Ivana and Kosanović, Dejana and Inić-Kanada, Aleksandra and Gavrović-Jankulović, Marija and Stojanović, Marijana",
year = "2016",
abstract = "Recombinant banana lectin isoform (rBanLec) attaches specifically to the mucosal surface, crosses the epithelial barrier and then directly affects the immune response in mouse colon. Structural characteristics, specificity and physiological impacts of rBanLec reported until now highly resemble those of its natural counterpart. Here, we demonstrated that a dose dependent stimulation of the colon with rBanLec skewed the immune response towards Th1/Th17 direction and this effect was counterbalanced by the rise in IL-10 production. Qualitative and quantitative characteristics of the established cytokine network were dependent on the applied rBanLec concentration. In addition, rBanLec enhanced local NO production and myeloperoxidase activity and promoted an increase in local IgA and IgG production. Stimulation with rBanLec can be beneficial in prevention of pathologies raised due to inappropriate cell-mediated immune response as well as in prevention of the pathogen invasion via the colon. (C) 2015 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Functional Foods",
title = "Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon",
pages = "78-68",
volume = "20",
doi = "10.1016/j.jff.2015.10.019"
}

Marinković, E., Lukić, I., Kosanović, D., Inić-Kanada, A., Gavrović-Jankulović, M.,& Stojanović, M.. (2016). Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon. in Journal of Functional Foods
Elsevier, Amsterdam., 20, 68-78.
https://doi.org/10.1016/j.jff.2015.10.019

Marinković E, Lukić I, Kosanović D, Inić-Kanada A, Gavrović-Jankulović M, Stojanović M. Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon. in Journal of Functional Foods. 2016;20:68-78.
doi:10.1016/j.jff.2015.10.019 .

Marinković, Emilija, Lukić, Ivana, Kosanović, Dejana, Inić-Kanada, Aleksandra, Gavrović-Jankulović, Marija, Stojanović, Marijana, "Recombinantly produced banana lectin isoform promotes balanced pro-inflammatory response in the colon" in Journal of Functional Foods, 20 (2016):68-78,
https://doi.org/10.1016/j.jff.2015.10.019 . .

TY  - JOUR
AU  - Belij-Rammerstorfer, Sandra
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Stein, Elisabeth
AU  - Montanaro, Jacqueline
AU  - Bintner, Nora
AU  - Schuerer, Nadine
AU  - Ghasemian, Ehsan
AU  - Kundi, Michael
AU  - Barisani-Asenbauer, Talin
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/470
AB  - The aim of this study was to determine whether infectious dose of Chlamydia caviae after repeated infections influences the immunological responses and subsequent clearance of pathogen at the ocular surface of guinea pigs. Animals were infected three times via the conjunctiva at six- and twelve-week intervals by applying either 1 x 10(4) or 1 x 10(6) inclusion-forming units (IFUs) of C. caviae. Ocular pathology, infection course, C. caviae-specific serum IgG levels and their capacity to bind and neutralize infection ex vivo were assessed. Animals infected with 1 x 10(4) IFUs had completely diminished ocular infection and pathology after the 2nd infection with increased levels of C. caviae-specific serum IgG and their effective capacity to bind and neutralize C. caviae. Only partial protection was observed in animals infected with 1 x 10(6) IFUs after the 2nd and 3rd infections. Our findings show that full protection was observed in animals repeatedly infected with the lower dose. The lower dose appeared not to compromise the host immune system, thereby enabling fast clearance of the pathogen and the establishment of competent neutralizing antibodies. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Microbes and Infection
T1  - Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection
EP  - 262
IS  - 4
SP  - 254
VL  - 18
DO  - 10.1016/j.micinf.2015.12.001
ER  - 

@article{
author = "Belij-Rammerstorfer, Sandra and Inić-Kanada, Aleksandra and Stojanović, Marijana and Marinković, Emilija and Lukić, Ivana and Stein, Elisabeth and Montanaro, Jacqueline and Bintner, Nora and Schuerer, Nadine and Ghasemian, Ehsan and Kundi, Michael and Barisani-Asenbauer, Talin",
year = "2016",
abstract = "The aim of this study was to determine whether infectious dose of Chlamydia caviae after repeated infections influences the immunological responses and subsequent clearance of pathogen at the ocular surface of guinea pigs. Animals were infected three times via the conjunctiva at six- and twelve-week intervals by applying either 1 x 10(4) or 1 x 10(6) inclusion-forming units (IFUs) of C. caviae. Ocular pathology, infection course, C. caviae-specific serum IgG levels and their capacity to bind and neutralize infection ex vivo were assessed. Animals infected with 1 x 10(4) IFUs had completely diminished ocular infection and pathology after the 2nd infection with increased levels of C. caviae-specific serum IgG and their effective capacity to bind and neutralize C. caviae. Only partial protection was observed in animals infected with 1 x 10(6) IFUs after the 2nd and 3rd infections. Our findings show that full protection was observed in animals repeatedly infected with the lower dose. The lower dose appeared not to compromise the host immune system, thereby enabling fast clearance of the pathogen and the establishment of competent neutralizing antibodies. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Microbes and Infection",
title = "Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection",
pages = "262-254",
number = "4",
volume = "18",
doi = "10.1016/j.micinf.2015.12.001"
}

Belij-Rammerstorfer, S., Inić-Kanada, A., Stojanović, M., Marinković, E., Lukić, I., Stein, E., Montanaro, J., Bintner, N., Schuerer, N., Ghasemian, E., Kundi, M.,& Barisani-Asenbauer, T.. (2016). Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection. in Microbes and Infection
Elsevier Science Bv, Amsterdam., 18(4), 254-262.
https://doi.org/10.1016/j.micinf.2015.12.001

Belij-Rammerstorfer S, Inić-Kanada A, Stojanović M, Marinković E, Lukić I, Stein E, Montanaro J, Bintner N, Schuerer N, Ghasemian E, Kundi M, Barisani-Asenbauer T. Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection. in Microbes and Infection. 2016;18(4):254-262.
doi:10.1016/j.micinf.2015.12.001 .

Belij-Rammerstorfer, Sandra, Inić-Kanada, Aleksandra, Stojanović, Marijana, Marinković, Emilija, Lukić, Ivana, Stein, Elisabeth, Montanaro, Jacqueline, Bintner, Nora, Schuerer, Nadine, Ghasemian, Ehsan, Kundi, Michael, Barisani-Asenbauer, Talin, "Infectious dose and repeated infections are key factors influencing immune response characteristics in guinea pig ocular chlamydial infection" in Microbes and Infection, 18, no. 4 (2016):254-262,
https://doi.org/10.1016/j.micinf.2015.12.001 . .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Glamoclija, Jasmina
AU  - Nikolić, Milos
AU  - Soković, Marina
AU  - Milošević, Verica
AU  - Jarić, Ivana
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Barisani-Asenbauer, Talin
AU  - Prodanović, Radivoje
AU  - Jovanović, Miloš
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/467
AB  - Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP-Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - International Journal of Antimicrobial Agents
T1  - Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment
EP  - 735
IS  - 6
SP  - 732
VL  - 48
DO  - 10.1016/j.ijantimicag.2016.08.014
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Glamoclija, Jasmina and Nikolić, Milos and Soković, Marina and Milošević, Verica and Jarić, Ivana and Stojanović, Marijana and Marinković, Emilija and Barisani-Asenbauer, Talin and Prodanović, Radivoje and Jovanović, Miloš and Radotić, Ksenija",
year = "2016",
abstract = "Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP-Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Antimicrobial Agents",
title = "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment",
pages = "735-732",
number = "6",
volume = "48",
doi = "10.1016/j.ijantimicag.2016.08.014"
}

Spasojević, D., Zmejkoski, D., Glamoclija, J., Nikolić, M., Soković, M., Milošević, V., Jarić, I., Stojanović, M., Marinković, E., Barisani-Asenbauer, T., Prodanović, R., Jovanović, M.,& Radotić, K.. (2016). Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. in International Journal of Antimicrobial Agents
Elsevier, Amsterdam., 48(6), 732-735.
https://doi.org/10.1016/j.ijantimicag.2016.08.014

Spasojević D, Zmejkoski D, Glamoclija J, Nikolić M, Soković M, Milošević V, Jarić I, Stojanović M, Marinković E, Barisani-Asenbauer T, Prodanović R, Jovanović M, Radotić K. Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. in International Journal of Antimicrobial Agents. 2016;48(6):732-735.
doi:10.1016/j.ijantimicag.2016.08.014 .

Spasojević, Dragica, Zmejkoski, Danica, Glamoclija, Jasmina, Nikolić, Milos, Soković, Marina, Milošević, Verica, Jarić, Ivana, Stojanović, Marijana, Marinković, Emilija, Barisani-Asenbauer, Talin, Prodanović, Radivoje, Jovanović, Miloš, Radotić, Ksenija, "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment" in International Journal of Antimicrobial Agents, 48, no. 6 (2016):732-735,
https://doi.org/10.1016/j.ijantimicag.2016.08.014 . .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stein, Elisabeth
AU  - Lukić, Ivana
AU  - Belij, Sandra
AU  - Schuerer, Nadine
AU  - Montanaro, Jacqueline
AU  - Ghasemian, Ehsan
AU  - Đokić, Radmila
AU  - Barisani-Asenbauer, Talin
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/459
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization
IS  - 12
VL  - 57
UR  - https://hdl.handle.net/21.15107/rcub_intor_459
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Marinković, Emilija and Stein, Elisabeth and Lukić, Ivana and Belij, Sandra and Schuerer, Nadine and Montanaro, Jacqueline and Ghasemian, Ehsan and Đokić, Radmila and Barisani-Asenbauer, Talin",
year = "2016",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization",
number = "12",
volume = "57",
url = "https://hdl.handle.net/21.15107/rcub_intor_459"
}

Inić-Kanada, A., Stojanović, M., Marinković, E., Stein, E., Lukić, I., Belij, S., Schuerer, N., Montanaro, J., Ghasemian, E., Đokić, R.,& Barisani-Asenbauer, T.. (2016). Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 57(12).
https://hdl.handle.net/21.15107/rcub_intor_459

Inić-Kanada A, Stojanović M, Marinković E, Stein E, Lukić I, Belij S, Schuerer N, Montanaro J, Ghasemian E, Đokić R, Barisani-Asenbauer T. Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization. in Investigative Ophthalmology & Visual Science. 2016;57(12).
https://hdl.handle.net/21.15107/rcub_intor_459 .

Inić-Kanada, Aleksandra, Stojanović, Marijana, Marinković, Emilija, Stein, Elisabeth, Lukić, Ivana, Belij, Sandra, Schuerer, Nadine, Montanaro, Jacqueline, Ghasemian, Ehsan, Đokić, Radmila, Barisani-Asenbauer, Talin, "Immune Responses Induced by chlamydial Polymorphic Membrane Protein C formulated with probiotic Lactobacillus rhamnosus as an Adjuvant in Conjunctival Immunization" in Investigative Ophthalmology & Visual Science, 57, no. 12 (2016),
https://hdl.handle.net/21.15107/rcub_intor_459 .

TY  - CONF
AU  - Schuerer, Nadine
AU  - Stein, Elisabeth
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Montanaro, Jacqueline
AU  - Ghasemian, Ehsan
AU  - Marinković, Emilija
AU  - Filipović, Ana
AU  - Barisani-Asenbauer, Talin
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/458
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo
IS  - 12
VL  - 57
UR  - https://hdl.handle.net/21.15107/rcub_intor_458
ER  - 

@conference{
author = "Schuerer, Nadine and Stein, Elisabeth and Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Montanaro, Jacqueline and Ghasemian, Ehsan and Marinković, Emilija and Filipović, Ana and Barisani-Asenbauer, Talin",
year = "2016",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo",
number = "12",
volume = "57",
url = "https://hdl.handle.net/21.15107/rcub_intor_458"
}

Schuerer, N., Stein, E., Inić-Kanada, A., Belij, S., Stojanović, M., Montanaro, J., Ghasemian, E., Marinković, E., Filipović, A.,& Barisani-Asenbauer, T.. (2016). Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 57(12).
https://hdl.handle.net/21.15107/rcub_intor_458

Schuerer N, Stein E, Inić-Kanada A, Belij S, Stojanović M, Montanaro J, Ghasemian E, Marinković E, Filipović A, Barisani-Asenbauer T. Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo. in Investigative Ophthalmology & Visual Science. 2016;57(12).
https://hdl.handle.net/21.15107/rcub_intor_458 .

Schuerer, Nadine, Stein, Elisabeth, Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Montanaro, Jacqueline, Ghasemian, Ehsan, Marinković, Emilija, Filipović, Ana, Barisani-Asenbauer, Talin, "Carrageenan - a natural inhibitor of ocular chlamydial infection in vitro and in vivo" in Investigative Ophthalmology & Visual Science, 57, no. 12 (2016),
https://hdl.handle.net/21.15107/rcub_intor_458 .

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Becker, Elisabeth
AU  - Stein, Elisabeth
AU  - Lukić, Ivana
AU  - Đokić, Radmila
AU  - Schuerer, Nadine
AU  - Hegemann, Johannes H.
AU  - Barisani-Asenbauer, Talin
PY  - 2016
UR  - http://intor.torlakinstitut.com/handle/123456789/455
AB  - Recent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving the way for new possibilities to oppose the societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate the risks associated with the use of a whole-cell vaccine. Our rationale for the design of an efficient subunit vaccine against Chlamydia trachomatis (Ct) is based on the membrane proteins involved in the initial Ct-host cell contact and on the route of immunization that mimics the natural infection process (i.e., via the ocular mucosa). The first aim of our study was to characterize the specific conjunctival and vaginal immune responses following eye drop immunization in BALB/c mice, using the N-terminal portion of the Ct serovar E polymorphic membrane protein C (N-PmpC) as the subunit vaccine antigen. Second, we aimed to examine the adjuvant properties of the probiotic Lactobacillus rhamnosus (LB) when formulated with N-PmpC. N-PmpC applied alone stimulated the production of N-PmpC-and Ct serovar B-specific antibodies in serum, tears and vaginal washes, whereas the combination with LB significantly enhanced these responses. The N-PmpC/LB combination initiated a T cell response characterized by an elevated percentage of CD25+ T cells and CD8+ effector T cells, enhanced CD4+ T-helper 1 skewing, and increased regulatory T cell responses. Together, these results show that eye drop vaccination with combined use of N-PmpC and a live probiotic LB stimulates specific cellular and humoral immune responses, not only locally in the conjunctiva but also in the vaginal mucosa, which could be a promising approach in Ct vaccine development.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0157875
ER  - 

@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Marinković, Emilija and Becker, Elisabeth and Stein, Elisabeth and Lukić, Ivana and Đokić, Radmila and Schuerer, Nadine and Hegemann, Johannes H. and Barisani-Asenbauer, Talin",
year = "2016",
abstract = "Recent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving the way for new possibilities to oppose the societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate the risks associated with the use of a whole-cell vaccine. Our rationale for the design of an efficient subunit vaccine against Chlamydia trachomatis (Ct) is based on the membrane proteins involved in the initial Ct-host cell contact and on the route of immunization that mimics the natural infection process (i.e., via the ocular mucosa). The first aim of our study was to characterize the specific conjunctival and vaginal immune responses following eye drop immunization in BALB/c mice, using the N-terminal portion of the Ct serovar E polymorphic membrane protein C (N-PmpC) as the subunit vaccine antigen. Second, we aimed to examine the adjuvant properties of the probiotic Lactobacillus rhamnosus (LB) when formulated with N-PmpC. N-PmpC applied alone stimulated the production of N-PmpC-and Ct serovar B-specific antibodies in serum, tears and vaginal washes, whereas the combination with LB significantly enhanced these responses. The N-PmpC/LB combination initiated a T cell response characterized by an elevated percentage of CD25+ T cells and CD8+ effector T cells, enhanced CD4+ T-helper 1 skewing, and increased regulatory T cell responses. Together, these results show that eye drop vaccination with combined use of N-PmpC and a live probiotic LB stimulates specific cellular and humoral immune responses, not only locally in the conjunctiva but also in the vaginal mucosa, which could be a promising approach in Ct vaccine development.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0157875"
}

Inić-Kanada, A., Stojanović, M., Marinković, E., Becker, E., Stein, E., Lukić, I., Đokić, R., Schuerer, N., Hegemann, J. H.,& Barisani-Asenbauer, T.. (2016). A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C. in PLoS One
Public Library Science, San Francisco., 11(9).
https://doi.org/10.1371/journal.pone.0157875

Inić-Kanada A, Stojanović M, Marinković E, Becker E, Stein E, Lukić I, Đokić R, Schuerer N, Hegemann JH, Barisani-Asenbauer T. A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C. in PLoS One. 2016;11(9).
doi:10.1371/journal.pone.0157875 .

Inić-Kanada, Aleksandra, Stojanović, Marijana, Marinković, Emilija, Becker, Elisabeth, Stein, Elisabeth, Lukić, Ivana, Đokić, Radmila, Schuerer, Nadine, Hegemann, Johannes H., Barisani-Asenbauer, Talin, "A Probiotic Adjuvant Lactobacillus rhamnosus Enhances Specific Immune Responses after Ocular Mucosal Immunization with Chlamydial Polymorphic Membrane Protein C" in PLoS One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0157875 . .

TY  - JOUR
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
AU  - Schlacher, Simone
AU  - Stein, Elisabeth
AU  - Belij-Rammerstorfer, Sandra
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Montanaro, Jacqueline
AU  - Schuerer, Nadine
AU  - Bintner, Nora
AU  - Kovačević-Jovanović, Vesna
AU  - Krnjaja, Ognjen
AU  - Mayr, Ulrike Beate
AU  - Lubitz, Werner
AU  - Barisani-Asenbauer, Talin
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/434
AB  - Trachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains the world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As the mechanisms engaged in the pathology of the disease are not fully understood, designing a subunit vaccine specific to chlamydial antigens could improve safety for human use. We propose the delivery of chlamydia-specific antigens to the ocular mucosa using particulate carriers, bacterial ghosts (BGs). We therefore characterized humoral and cellular immune responses after conjunctival and subcutaneous immunization with a N-terminal portion (amino acid 1-893) of the chlamydial polymorphic membrane protein C (PmpC) of Ct serovar B, expressed in probiotic Escherichia coli Nissle 1917 bacterial ghosts (EcN BGs) in BALB/cmice. Three immunizations were performed at two-week intervals, and the immune responses were evaluated two weeks after the final immunization in mice. In a guinea pig model of ocular infection animals were immunized in the same manner as the mice, and protection against challenge was assessed two weeks after the last immunization. N-PmpC was successfully expressed within BGs and delivery to the ocularmucosa was well tolerated without signs of inflammation. N-PmpC- specific mucosal IgA levels in tears yielded significantly increased levels in the group immunized via the conjunctiva compared with the subcutaneously immunized mice. Immunization with N-PmpC EcN BGs via both immunization routes prompted the establishment of an N-PmpC-specific IFN gamma immune response. Immunization via the conjunctiva resulted in a decrease in intensity of the transitional inflammatory reaction in conjunctiva of challenged guinea pigs compared with subcutaneously and non-immunized animals. The delivery of the chlamydial subunit vaccine to the ocular mucosa using a particulate carrier, such as BGs, induced both humoral and cellular immune responses. Further investigations are needed to improve the immunization scheme and dosage.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers
IS  - 12
VL  - 10
DO  - 10.1371/journal.pone.0144380
ER  - 

@article{
author = "Inić-Kanada, Aleksandra and Stojanović, Marijana and Schlacher, Simone and Stein, Elisabeth and Belij-Rammerstorfer, Sandra and Marinković, Emilija and Lukić, Ivana and Montanaro, Jacqueline and Schuerer, Nadine and Bintner, Nora and Kovačević-Jovanović, Vesna and Krnjaja, Ognjen and Mayr, Ulrike Beate and Lubitz, Werner and Barisani-Asenbauer, Talin",
year = "2015",
abstract = "Trachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains the world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As the mechanisms engaged in the pathology of the disease are not fully understood, designing a subunit vaccine specific to chlamydial antigens could improve safety for human use. We propose the delivery of chlamydia-specific antigens to the ocular mucosa using particulate carriers, bacterial ghosts (BGs). We therefore characterized humoral and cellular immune responses after conjunctival and subcutaneous immunization with a N-terminal portion (amino acid 1-893) of the chlamydial polymorphic membrane protein C (PmpC) of Ct serovar B, expressed in probiotic Escherichia coli Nissle 1917 bacterial ghosts (EcN BGs) in BALB/cmice. Three immunizations were performed at two-week intervals, and the immune responses were evaluated two weeks after the final immunization in mice. In a guinea pig model of ocular infection animals were immunized in the same manner as the mice, and protection against challenge was assessed two weeks after the last immunization. N-PmpC was successfully expressed within BGs and delivery to the ocularmucosa was well tolerated without signs of inflammation. N-PmpC- specific mucosal IgA levels in tears yielded significantly increased levels in the group immunized via the conjunctiva compared with the subcutaneously immunized mice. Immunization with N-PmpC EcN BGs via both immunization routes prompted the establishment of an N-PmpC-specific IFN gamma immune response. Immunization via the conjunctiva resulted in a decrease in intensity of the transitional inflammatory reaction in conjunctiva of challenged guinea pigs compared with subcutaneously and non-immunized animals. The delivery of the chlamydial subunit vaccine to the ocular mucosa using a particulate carrier, such as BGs, induced both humoral and cellular immune responses. Further investigations are needed to improve the immunization scheme and dosage.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers",
number = "12",
volume = "10",
doi = "10.1371/journal.pone.0144380"
}

Inić-Kanada, A., Stojanović, M., Schlacher, S., Stein, E., Belij-Rammerstorfer, S., Marinković, E., Lukić, I., Montanaro, J., Schuerer, N., Bintner, N., Kovačević-Jovanović, V., Krnjaja, O., Mayr, U. B., Lubitz, W.,& Barisani-Asenbauer, T.. (2015). Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers. in PLoS One
Public Library Science, San Francisco., 10(12).
https://doi.org/10.1371/journal.pone.0144380

Inić-Kanada A, Stojanović M, Schlacher S, Stein E, Belij-Rammerstorfer S, Marinković E, Lukić I, Montanaro J, Schuerer N, Bintner N, Kovačević-Jovanović V, Krnjaja O, Mayr UB, Lubitz W, Barisani-Asenbauer T. Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers. in PLoS One. 2015;10(12).
doi:10.1371/journal.pone.0144380 .

Inić-Kanada, Aleksandra, Stojanović, Marijana, Schlacher, Simone, Stein, Elisabeth, Belij-Rammerstorfer, Sandra, Marinković, Emilija, Lukić, Ivana, Montanaro, Jacqueline, Schuerer, Nadine, Bintner, Nora, Kovačević-Jovanović, Vesna, Krnjaja, Ognjen, Mayr, Ulrike Beate, Lubitz, Werner, Barisani-Asenbauer, Talin, "Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers" in PLoS One, 10, no. 12 (2015),
https://doi.org/10.1371/journal.pone.0144380 . .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Filipović, Ana
AU  - Krnjaja, Ognjen
AU  - Kosanović, Dejana
AU  - Inić-Kanada, Aleksandra
AU  - Stojanović, Marijana
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/425
AB  - Antibodies capable to neutralize tetanus toxin (TeNT) are key factors in protection against tetanus disease. Although antibody-based therapeutics for treatment of tetanus exist on the market its production is tedious. Hence, the tetanus-specific antibodies preparation that could be easily produced in large scale in vitro would be beneficial. Monoclonal antibodies (MAbs) are considered for a long time as a reagent of choice, but the core drawback is how to select a MAb that would be safe in providing efficacious protection. In this study we have investigated the parameters crucial for a single MAb to be assigned as protective. Eight murine MAbs were characterized in vitro for their reactivity toward TeNT and assessed in vivo for protectiveness against TeNT intoxication. Correlation of in vitro and in vivo data has revealed that in vitro selection of MAb that is protective in vivo could be performed by a combination of two assays: the measurement of MAb affinity toward TeNT taking Ka 1 x 10(8) M-1 as a threshold level, and the evaluation of its capability to prevent TeNT-ganglioside interaction. Single MAb could be taken into consideration as a potential therapeutic only if it has a capacity to completely inhibits TeNT-ganglioside complex formation. (C) 2015 Elsevier Ltd. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Toxicon
T1  - Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction
EP  - 144
SP  - 135
VL  - 103
DO  - 10.1016/j.toxicon.2015.06.025
ER  - 

@article{
author = "Lukić, Ivana and Marinković, Emilija and Filipović, Ana and Krnjaja, Ognjen and Kosanović, Dejana and Inić-Kanada, Aleksandra and Stojanović, Marijana",
year = "2015",
abstract = "Antibodies capable to neutralize tetanus toxin (TeNT) are key factors in protection against tetanus disease. Although antibody-based therapeutics for treatment of tetanus exist on the market its production is tedious. Hence, the tetanus-specific antibodies preparation that could be easily produced in large scale in vitro would be beneficial. Monoclonal antibodies (MAbs) are considered for a long time as a reagent of choice, but the core drawback is how to select a MAb that would be safe in providing efficacious protection. In this study we have investigated the parameters crucial for a single MAb to be assigned as protective. Eight murine MAbs were characterized in vitro for their reactivity toward TeNT and assessed in vivo for protectiveness against TeNT intoxication. Correlation of in vitro and in vivo data has revealed that in vitro selection of MAb that is protective in vivo could be performed by a combination of two assays: the measurement of MAb affinity toward TeNT taking Ka 1 x 10(8) M-1 as a threshold level, and the evaluation of its capability to prevent TeNT-ganglioside interaction. Single MAb could be taken into consideration as a potential therapeutic only if it has a capacity to completely inhibits TeNT-ganglioside complex formation. (C) 2015 Elsevier Ltd. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicon",
title = "Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction",
pages = "144-135",
volume = "103",
doi = "10.1016/j.toxicon.2015.06.025"
}

Lukić, I., Marinković, E., Filipović, A., Krnjaja, O., Kosanović, D., Inić-Kanada, A.,& Stojanović, M.. (2015). Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction. in Toxicon
Pergamon-Elsevier Science Ltd, Oxford., 103, 135-144.
https://doi.org/10.1016/j.toxicon.2015.06.025

Lukić I, Marinković E, Filipović A, Krnjaja O, Kosanović D, Inić-Kanada A, Stojanović M. Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction. in Toxicon. 2015;103:135-144.
doi:10.1016/j.toxicon.2015.06.025 .

Lukić, Ivana, Marinković, Emilija, Filipović, Ana, Krnjaja, Ognjen, Kosanović, Dejana, Inić-Kanada, Aleksandra, Stojanović, Marijana, "Key protection factors against tetanus: Anti-tetanus toxin antibody affinity and its ability to prevent tetanus toxin - ganglioside interaction" in Toxicon, 103 (2015):135-144,
https://doi.org/10.1016/j.toxicon.2015.06.025 . .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Stein, Elisabeth
AU  - Ladurner, Angela
AU  - Mayr, Ulrike Beate
AU  - Lubitz, Werner
AU  - Barisani-Asenbauer, Talin
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/415
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts
IS  - 13
VL  - 55
UR  - https://hdl.handle.net/21.15107/rcub_intor_415
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Stein, Elisabeth and Ladurner, Angela and Mayr, Ulrike Beate and Lubitz, Werner and Barisani-Asenbauer, Talin",
year = "2014",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts",
number = "13",
volume = "55",
url = "https://hdl.handle.net/21.15107/rcub_intor_415"
}

Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Stein, E., Ladurner, A., Mayr, U. B., Lubitz, W.,& Barisani-Asenbauer, T.. (2014). The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 55(13).
https://hdl.handle.net/21.15107/rcub_intor_415

Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Stein E, Ladurner A, Mayr UB, Lubitz W, Barisani-Asenbauer T. The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts. in Investigative Ophthalmology & Visual Science. 2014;55(13).
https://hdl.handle.net/21.15107/rcub_intor_415 .

Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Stein, Elisabeth, Ladurner, Angela, Mayr, Ulrike Beate, Lubitz, Werner, Barisani-Asenbauer, Talin, "The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts" in Investigative Ophthalmology & Visual Science, 55, no. 13 (2014),
https://hdl.handle.net/21.15107/rcub_intor_415 .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Montanaro, Jacqueline
AU  - Stein, Elisabeth
AU  - Bintner, Nora
AU  - Schuerer, Nadine
AU  - Ladurner, Angela
AU  - Barisani-Asenbauer, Talin
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/404
PB  - Wiley-Blackwell, Hoboken
C3  - Immunology
T1  - Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis
EP  - 25
SP  - 25
VL  - 143
UR  - https://hdl.handle.net/21.15107/rcub_intor_404
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Montanaro, Jacqueline and Stein, Elisabeth and Bintner, Nora and Schuerer, Nadine and Ladurner, Angela and Barisani-Asenbauer, Talin",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Immunology",
title = "Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis",
pages = "25-25",
volume = "143",
url = "https://hdl.handle.net/21.15107/rcub_intor_404"
}

Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Montanaro, J., Stein, E., Bintner, N., Schuerer, N., Ladurner, A.,& Barisani-Asenbauer, T.. (2014). Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis. in Immunology
Wiley-Blackwell, Hoboken., 143, 25-25.
https://hdl.handle.net/21.15107/rcub_intor_404

Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Montanaro J, Stein E, Bintner N, Schuerer N, Ladurner A, Barisani-Asenbauer T. Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis. in Immunology. 2014;143:25-25.
https://hdl.handle.net/21.15107/rcub_intor_404 .

Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Montanaro, Jacqueline, Stein, Elisabeth, Bintner, Nora, Schuerer, Nadine, Ladurner, Angela, Barisani-Asenbauer, Talin, "Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis" in Immunology, 143 (2014):25-25,
https://hdl.handle.net/21.15107/rcub_intor_404 .

TY  - JOUR
AU  - Stojanović, Marijana
AU  - Petrušić, Vladimir
AU  - Živković, Irena
AU  - Inić-Kanada, Aleksandra
AU  - Stojičević, Ivana
AU  - Marinković, Emilija
AU  - Dimitrijević, Ljiljana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/395
AB  - It is known that tetanus toxoid (TTd)-hyperimmunization induces increased titer of sera beta 2-glycoprotein I (beta 2GPI)-specific antibodies (Abs) in Balb/c mice. The concentrations of such induced anti-beta 2GPI Abs as well as their pathogenic potential are strongly influenced by the context of TTd application. beta 2GPI-specific immune response is established as a part of TTd-specific immune response by molecular mimicry mechanism due to structural homology between TTd and beta 2GPI. This finding is supported by the following facts: (1) cross-reactive Abs that recognize both TTd and beta 2GPI epitopes are present in Balb/c mice sera; (2) anti-TTd Abs secretion in splenic cultures is induced after beta 2GPI stimulation and vice versa. However, analyses of (1) IL-10 production following in vitro stimulation of immunized Balb/c mice splenocytes by TTd, beta 2GPI or glutaraldehyde-treated beta 2GPI and (2) specific impact of ConA and agonists of TLR2, TLR4, and TLR9 on anti-TTd and autoreactive Abs secretion strongly imply that these two branches of the TTd-induced immune response do not use identical cell populations and are regulated in a different way. Results presented in this paper describe that structural homology between foreign and self-antigens could focus mounted autoreactive immune response toward specific self-structure, but the context of antigen application, including a history of previous immune stimulations and adjuvants applied together with the antigen, are the main factors which determine the outcome of the induced immune response.
PB  - Humana Press Inc, Totowa
T2  - Immunologic Research
T1  - Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid
EP  - 31
IS  - 1
SP  - 20
VL  - 56
DO  - 10.1007/s12026-012-8343-1
ER  - 

@article{
author = "Stojanović, Marijana and Petrušić, Vladimir and Živković, Irena and Inić-Kanada, Aleksandra and Stojičević, Ivana and Marinković, Emilija and Dimitrijević, Ljiljana",
year = "2013",
abstract = "It is known that tetanus toxoid (TTd)-hyperimmunization induces increased titer of sera beta 2-glycoprotein I (beta 2GPI)-specific antibodies (Abs) in Balb/c mice. The concentrations of such induced anti-beta 2GPI Abs as well as their pathogenic potential are strongly influenced by the context of TTd application. beta 2GPI-specific immune response is established as a part of TTd-specific immune response by molecular mimicry mechanism due to structural homology between TTd and beta 2GPI. This finding is supported by the following facts: (1) cross-reactive Abs that recognize both TTd and beta 2GPI epitopes are present in Balb/c mice sera; (2) anti-TTd Abs secretion in splenic cultures is induced after beta 2GPI stimulation and vice versa. However, analyses of (1) IL-10 production following in vitro stimulation of immunized Balb/c mice splenocytes by TTd, beta 2GPI or glutaraldehyde-treated beta 2GPI and (2) specific impact of ConA and agonists of TLR2, TLR4, and TLR9 on anti-TTd and autoreactive Abs secretion strongly imply that these two branches of the TTd-induced immune response do not use identical cell populations and are regulated in a different way. Results presented in this paper describe that structural homology between foreign and self-antigens could focus mounted autoreactive immune response toward specific self-structure, but the context of antigen application, including a history of previous immune stimulations and adjuvants applied together with the antigen, are the main factors which determine the outcome of the induced immune response.",
publisher = "Humana Press Inc, Totowa",
journal = "Immunologic Research",
title = "Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid",
pages = "31-20",
number = "1",
volume = "56",
doi = "10.1007/s12026-012-8343-1"
}

Stojanović, M., Petrušić, V., Živković, I., Inić-Kanada, A., Stojičević, I., Marinković, E.,& Dimitrijević, L.. (2013). Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid. in Immunologic Research
Humana Press Inc, Totowa., 56(1), 20-31.
https://doi.org/10.1007/s12026-012-8343-1

Stojanović M, Petrušić V, Živković I, Inić-Kanada A, Stojičević I, Marinković E, Dimitrijević L. Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid. in Immunologic Research. 2013;56(1):20-31.
doi:10.1007/s12026-012-8343-1 .

Stojanović, Marijana, Petrušić, Vladimir, Živković, Irena, Inić-Kanada, Aleksandra, Stojičević, Ivana, Marinković, Emilija, Dimitrijević, Ljiljana, "Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid" in Immunologic Research, 56, no. 1 (2013):20-31,
https://doi.org/10.1007/s12026-012-8343-1 . .

TY  - JOUR
AU  - Barisani-Asenbauer, Talin
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Marinković, Emilija
AU  - Lukić, Ivana
AU  - Montanaro, Jacqueline
AU  - Stein, Elisabeth
AU  - Bintner, Nora
AU  - Stojanović, Marijana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/393
AB  - Background: In a quest for a needle-free vaccine administration strategy, we evaluated the ocular conjunctiva as an alternative mucosal immunization route by profiling and comparing the local and systemic immune responses to the subcutaneous or conjunctival administration of tetanus toxoid (TTd), a model antigen. Materials and methods: BALB/c and C57BL/6 mice were immunized either subcutaneously with TTd alone or via the conjunctiva with TTd alone, TTd mixed with 2% glycerol or TTd with merthiolate-inactivated whole-cell B. pertussis (wBP) as adjuvants. Mice were immunized on days 0, 7 and 14 via both routes, and an evaluation of the local and systemic immune responses was performed two weeks after the last immunization. Four weeks after the last immunization, the mice were challenged with a lethal dose (2 x LD50) of tetanus toxin. Results: The conjunctival application of TTd in BALB/c mice induced TTd-specific secretory IgA production and skewed the TTd-specific immune response toward a Th1/Th17 profile, as determined by the stimulation of IFN gamma and IL-17A secretion and/or the concurrent pronounced reduction of IL-4 secretion, irrespective of the adjuvant. In conjunctivaly immunized C57BL/6 mice, only TTd administered with wBP promoted the establishment of a mixed Th1/Th17 TTd-specific immune response, whereas TTd alone or TTd in conjunction with glycerol initiated a dominant Th1 response against TTd. Immunization via the conjunctiva with TTd plus wBP adjuvant resulted in a 33% survival rate of challenged mice compared to a 0% survival rate in non-immunized animals (p lt 0.05). Conclusion: Conjunctival immunization with TTd alone or with various adjuvants induced TTd-specific local and systemic immune responses, predominantly of the Th1 type. The strongest immune responses developed in mice that received TTd together with wBP, which implies that this alternative route might tailor the immune response to fight intracellular bacteria or viruses more effectively.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - The Ocular Conjunctiva as a Mucosal Immunization Route: A Profile of the Immune Response to the Model Antigen Tetanus Toxoid
IS  - 4
VL  - 8
DO  - 10.1371/journal.pone.0060682
ER  - 

@article{
author = "Barisani-Asenbauer, Talin and Inić-Kanada, Aleksandra and Belij, Sandra and Marinković, Emilija and Lukić, Ivana and Montanaro, Jacqueline and Stein, Elisabeth and Bintner, Nora and Stojanović, Marijana",
year = "2013",
abstract = "Background: In a quest for a needle-free vaccine administration strategy, we evaluated the ocular conjunctiva as an alternative mucosal immunization route by profiling and comparing the local and systemic immune responses to the subcutaneous or conjunctival administration of tetanus toxoid (TTd), a model antigen. Materials and methods: BALB/c and C57BL/6 mice were immunized either subcutaneously with TTd alone or via the conjunctiva with TTd alone, TTd mixed with 2% glycerol or TTd with merthiolate-inactivated whole-cell B. pertussis (wBP) as adjuvants. Mice were immunized on days 0, 7 and 14 via both routes, and an evaluation of the local and systemic immune responses was performed two weeks after the last immunization. Four weeks after the last immunization, the mice were challenged with a lethal dose (2 x LD50) of tetanus toxin. Results: The conjunctival application of TTd in BALB/c mice induced TTd-specific secretory IgA production and skewed the TTd-specific immune response toward a Th1/Th17 profile, as determined by the stimulation of IFN gamma and IL-17A secretion and/or the concurrent pronounced reduction of IL-4 secretion, irrespective of the adjuvant. In conjunctivaly immunized C57BL/6 mice, only TTd administered with wBP promoted the establishment of a mixed Th1/Th17 TTd-specific immune response, whereas TTd alone or TTd in conjunction with glycerol initiated a dominant Th1 response against TTd. Immunization via the conjunctiva with TTd plus wBP adjuvant resulted in a 33% survival rate of challenged mice compared to a 0% survival rate in non-immunized animals (p lt 0.05). Conclusion: Conjunctival immunization with TTd alone or with various adjuvants induced TTd-specific local and systemic immune responses, predominantly of the Th1 type. The strongest immune responses developed in mice that received TTd together with wBP, which implies that this alternative route might tailor the immune response to fight intracellular bacteria or viruses more effectively.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "The Ocular Conjunctiva as a Mucosal Immunization Route: A Profile of the Immune Response to the Model Antigen Tetanus Toxoid",
number = "4",
volume = "8",
doi = "10.1371/journal.pone.0060682"
}

Barisani-Asenbauer, T., Inić-Kanada, A., Belij, S., Marinković, E., Lukić, I., Montanaro, J., Stein, E., Bintner, N.,& Stojanović, M.. (2013). The Ocular Conjunctiva as a Mucosal Immunization Route: A Profile of the Immune Response to the Model Antigen Tetanus Toxoid. in PLoS One
Public Library Science, San Francisco., 8(4).
https://doi.org/10.1371/journal.pone.0060682

Barisani-Asenbauer T, Inić-Kanada A, Belij S, Marinković E, Lukić I, Montanaro J, Stein E, Bintner N, Stojanović M. The Ocular Conjunctiva as a Mucosal Immunization Route: A Profile of the Immune Response to the Model Antigen Tetanus Toxoid. in PLoS One. 2013;8(4).
doi:10.1371/journal.pone.0060682 .

Barisani-Asenbauer, Talin, Inić-Kanada, Aleksandra, Belij, Sandra, Marinković, Emilija, Lukić, Ivana, Montanaro, Jacqueline, Stein, Elisabeth, Bintner, Nora, Stojanović, Marijana, "The Ocular Conjunctiva as a Mucosal Immunization Route: A Profile of the Immune Response to the Model Antigen Tetanus Toxoid" in PLoS One, 8, no. 4 (2013),
https://doi.org/10.1371/journal.pone.0060682 . .

TY  - JOUR
AU  - Petrušić, Vladimir
AU  - Živković, Irena
AU  - Stojanović, Marijana
AU  - Lukić, Ivana
AU  - Marinković, Emilija
AU  - Dimitrijević, Ljiljana
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/349
AB  - Tetanus neurotoxin (TeNT) represents a potent toxin that binds to its receptors on neurons and inhibits the release of neurotransmitters. Additionally, its fragments are used to transport pharmacological substances to neuronal cell bodies. The main objective of this study was the development of a suitable model system to study internalization of the TeNT. We have produced a monoclonal antibody (MoAb) specific for TeNT by hybridoma technology, after immunization of BALB/c mice with tetanus toxoid, and have named it T-62. The immunochemical characteristics of MoAb T-62 were tested using ELISA, PAGE and immunoblotting. Finally, we have used an immunohistochemical method to detect specific binding of MoAb T-62 to TeNT bound to PC 12 cells. Our results show that MoAb T-62 is highly specific for TeNT, even when it is bound to its receptor, and that it could be of considerable importance in studies regarding fundamental research on TeNT receptors, intracellular transport of TeNT, as well as retrograde transport of pharmaceutical substances and non-invasive delivery of polypeptides through the blood brain barrier. In addition, MoAb T-62 is an invaluable tool in TeNT vaccine production as it can be used for the detection of reverse toxicity, which could drastically reduce the need to use animals in these experiments. (C) 2011 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Acta Histochemica
T1  - Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62
EP  - 486
IS  - 5
SP  - 480
VL  - 114
DO  - 10.1016/j.acthis.2011.09.001
ER  - 

@article{
author = "Petrušić, Vladimir and Živković, Irena and Stojanović, Marijana and Lukić, Ivana and Marinković, Emilija and Dimitrijević, Ljiljana",
year = "2012",
abstract = "Tetanus neurotoxin (TeNT) represents a potent toxin that binds to its receptors on neurons and inhibits the release of neurotransmitters. Additionally, its fragments are used to transport pharmacological substances to neuronal cell bodies. The main objective of this study was the development of a suitable model system to study internalization of the TeNT. We have produced a monoclonal antibody (MoAb) specific for TeNT by hybridoma technology, after immunization of BALB/c mice with tetanus toxoid, and have named it T-62. The immunochemical characteristics of MoAb T-62 were tested using ELISA, PAGE and immunoblotting. Finally, we have used an immunohistochemical method to detect specific binding of MoAb T-62 to TeNT bound to PC 12 cells. Our results show that MoAb T-62 is highly specific for TeNT, even when it is bound to its receptor, and that it could be of considerable importance in studies regarding fundamental research on TeNT receptors, intracellular transport of TeNT, as well as retrograde transport of pharmaceutical substances and non-invasive delivery of polypeptides through the blood brain barrier. In addition, MoAb T-62 is an invaluable tool in TeNT vaccine production as it can be used for the detection of reverse toxicity, which could drastically reduce the need to use animals in these experiments. (C) 2011 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Acta Histochemica",
title = "Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62",
pages = "486-480",
number = "5",
volume = "114",
doi = "10.1016/j.acthis.2011.09.001"
}

Petrušić, V., Živković, I., Stojanović, M., Lukić, I., Marinković, E.,& Dimitrijević, L.. (2012). Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62. in Acta Histochemica
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 114(5), 480-486.
https://doi.org/10.1016/j.acthis.2011.09.001

Petrušić V, Živković I, Stojanović M, Lukić I, Marinković E, Dimitrijević L. Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62. in Acta Histochemica. 2012;114(5):480-486.
doi:10.1016/j.acthis.2011.09.001 .

Petrušić, Vladimir, Živković, Irena, Stojanović, Marijana, Lukić, Ivana, Marinković, Emilija, Dimitrijević, Ljiljana, "Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62" in Acta Histochemica, 114, no. 5 (2012):480-486,
https://doi.org/10.1016/j.acthis.2011.09.001 . .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Montanaro, Jacqueline
AU  - Schlacher, Simone
AU  - Stein, Elisabeth
AU  - Bintner, Nora
AU  - Barisani-Asenbauer, Talin
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/341
PB  - Wiley-Blackwell, Hoboken
C3  - Immunology
T1  - Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen
EP  - 767
SP  - 767
VL  - 137
UR  - https://hdl.handle.net/21.15107/rcub_intor_341
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Montanaro, Jacqueline and Schlacher, Simone and Stein, Elisabeth and Bintner, Nora and Barisani-Asenbauer, Talin",
year = "2012",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Immunology",
title = "Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen",
pages = "767-767",
volume = "137",
url = "https://hdl.handle.net/21.15107/rcub_intor_341"
}

Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Montanaro, J., Schlacher, S., Stein, E., Bintner, N.,& Barisani-Asenbauer, T.. (2012). Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen. in Immunology
Wiley-Blackwell, Hoboken., 137, 767-767.
https://hdl.handle.net/21.15107/rcub_intor_341

Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Montanaro J, Schlacher S, Stein E, Bintner N, Barisani-Asenbauer T. Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen. in Immunology. 2012;137:767-767.
https://hdl.handle.net/21.15107/rcub_intor_341 .

Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Montanaro, Jacqueline, Schlacher, Simone, Stein, Elisabeth, Bintner, Nora, Barisani-Asenbauer, Talin, "Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen" in Immunology, 137 (2012):767-767,
https://hdl.handle.net/21.15107/rcub_intor_341 .