TY  - CONF
AU  - Milovanović, Mina
AU  - Atanasković-Marković, Marina
AU  - Vučković, Olga
AU  - Becker, Wolf-Meinhard
AU  - Petersen, Arnd
AU  - Gavrović-Jankulović, Marija
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/271
PB  - Wiley-Blackwell Publishing, Inc, Malden
C3  - Allergy
T1  - Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy
EP  - 80
SP  - 80
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_intor_271
ER  - 

@conference{
author = "Milovanović, Mina and Atanasković-Marković, Marina and Vučković, Olga and Becker, Wolf-Meinhard and Petersen, Arnd and Gavrović-Jankulović, Marija",
year = "2009",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "Allergy",
title = "Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy",
pages = "80-80",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_intor_271"
}

Milovanović, M., Atanasković-Marković, M., Vučković, O., Becker, W., Petersen, A.,& Gavrović-Jankulović, M.. (2009). Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy. in Allergy
Wiley-Blackwell Publishing, Inc, Malden., 64, 80-80.
https://hdl.handle.net/21.15107/rcub_intor_271

Milovanović M, Atanasković-Marković M, Vučković O, Becker W, Petersen A, Gavrović-Jankulović M. Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy. in Allergy. 2009;64:80-80.
https://hdl.handle.net/21.15107/rcub_intor_271 .

Milovanović, Mina, Atanasković-Marković, Marina, Vučković, Olga, Becker, Wolf-Meinhard, Petersen, Arnd, Gavrović-Jankulović, Marija, "Genetically engineered Phl p 1 is a suitable diagnostic marker for in vivo allergy diagnosis of timothy grass pollen allergy" in Allergy, 64 (2009):80-80,
https://hdl.handle.net/21.15107/rcub_intor_271 .

TY  - JOUR
AU  - Gavrović-Jankulović, Marija
AU  - Spasic, Milena
AU  - Veličković, Tanja
AU  - Stojanović, Marijana
AU  - Inić-Kanada, Aleksandra
AU  - Dimitrijevic, Ljiliana
AU  - Lindner, Buko
AU  - Petersen, Arnd
AU  - Becker, Wolf-Meinhard
AU  - Jankov, Ratko
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/248
AB  - Thaumatin-like proteins (TLPs) have been established as a new family of fruit and pollen allergens. The aim of this study was to develop a two-site ELISA for the quantification of the thaumatin-like kiwi allergen (Act d 2) in kiwifruit extracts and kiwifruit-containing food products. Genomic DNA (gDNA) of Act d 2 was amplified and the deduced amino acid sequence was determined to obtain a primary structure. Act d 2 purified from kiwifruit extract by HPLC was identified by Edman degradation and MS. Balb/c mice were immunized with Act d 2 for the production of mAbs by hybridoma technology. The optimized ELISA measured Act d 2 concentrations ranging from 0.2 to 9.0 ng/mL, with intra- and interassay coefficients of variation of 3.65 and 10.44%, respectively. The developed ELISA is a useful method for the quantification of the thaumatin-like kiwi allergen in kiwifruit extracts as well as the allergen level in kiwifruit-containing food products. It may be a helpful analytical tool for the evaluation of the stability (integrity) of fruit allergen extracts for in vitro diagnosis.
T2  - Molecular Nutrition and Food Research
T1  - Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA
EP  - 707
IS  - 6
SP  - 701
VL  - 52
DO  - 10.1002/mnfr.200700286
ER  - 

@article{
author = "Gavrović-Jankulović, Marija and Spasic, Milena and Veličković, Tanja and Stojanović, Marijana and Inić-Kanada, Aleksandra and Dimitrijevic, Ljiliana and Lindner, Buko and Petersen, Arnd and Becker, Wolf-Meinhard and Jankov, Ratko",
year = "2008",
abstract = "Thaumatin-like proteins (TLPs) have been established as a new family of fruit and pollen allergens. The aim of this study was to develop a two-site ELISA for the quantification of the thaumatin-like kiwi allergen (Act d 2) in kiwifruit extracts and kiwifruit-containing food products. Genomic DNA (gDNA) of Act d 2 was amplified and the deduced amino acid sequence was determined to obtain a primary structure. Act d 2 purified from kiwifruit extract by HPLC was identified by Edman degradation and MS. Balb/c mice were immunized with Act d 2 for the production of mAbs by hybridoma technology. The optimized ELISA measured Act d 2 concentrations ranging from 0.2 to 9.0 ng/mL, with intra- and interassay coefficients of variation of 3.65 and 10.44%, respectively. The developed ELISA is a useful method for the quantification of the thaumatin-like kiwi allergen in kiwifruit extracts as well as the allergen level in kiwifruit-containing food products. It may be a helpful analytical tool for the evaluation of the stability (integrity) of fruit allergen extracts for in vitro diagnosis.",
journal = "Molecular Nutrition and Food Research",
title = "Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA",
pages = "707-701",
number = "6",
volume = "52",
doi = "10.1002/mnfr.200700286"
}

Gavrović-Jankulović, M., Spasic, M., Veličković, T., Stojanović, M., Inić-Kanada, A., Dimitrijevic, L., Lindner, B., Petersen, A., Becker, W.,& Jankov, R.. (2008). Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA. in Molecular Nutrition and Food Research, 52(6), 701-707.
https://doi.org/10.1002/mnfr.200700286

Gavrović-Jankulović M, Spasic M, Veličković T, Stojanović M, Inić-Kanada A, Dimitrijevic L, Lindner B, Petersen A, Becker W, Jankov R. Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA. in Molecular Nutrition and Food Research. 2008;52(6):701-707.
doi:10.1002/mnfr.200700286 .

Gavrović-Jankulović, Marija, Spasic, Milena, Veličković, Tanja, Stojanović, Marijana, Inić-Kanada, Aleksandra, Dimitrijevic, Ljiliana, Lindner, Buko, Petersen, Arnd, Becker, Wolf-Meinhard, Jankov, Ratko, "Quantification of the thaumatin-like kiwi allergen by a monoclonal antibody-based ELISA" in Molecular Nutrition and Food Research, 52, no. 6 (2008):701-707,
https://doi.org/10.1002/mnfr.200700286 . .

TY  - CONF
AU  - Popović, Milica
AU  - Burazer, Lidija
AU  - Atanasković-Marković, Marina
AU  - Milovanović, Mina
AU  - Ćirković-Veličković, Tanja
AU  - Petersen, Arnd
AU  - Jankov, Ratko
AU  - Becker, Wolf-Meinhard
AU  - Gavrović-Jankulović, Marija
PY  - 2008
UR  - http://intor.torlakinstitut.com/handle/123456789/255
PB  - Blackwell Publishing, Oxford
C3  - Allergy
T1  - A recombinant kiwi cystatin is a novel reagent for evaluation of the clinical relevance on phytocystatins in kiwi fruit allergy
EP  - 573
SP  - 573
VL  - 63
UR  - https://hdl.handle.net/21.15107/rcub_intor_255
ER  - 

@conference{
author = "Popović, Milica and Burazer, Lidija and Atanasković-Marković, Marina and Milovanović, Mina and Ćirković-Veličković, Tanja and Petersen, Arnd and Jankov, Ratko and Becker, Wolf-Meinhard and Gavrović-Jankulović, Marija",
year = "2008",
publisher = "Blackwell Publishing, Oxford",
journal = "Allergy",
title = "A recombinant kiwi cystatin is a novel reagent for evaluation of the clinical relevance on phytocystatins in kiwi fruit allergy",
pages = "573-573",
volume = "63",
url = "https://hdl.handle.net/21.15107/rcub_intor_255"
}

Popović, M., Burazer, L., Atanasković-Marković, M., Milovanović, M., Ćirković-Veličković, T., Petersen, A., Jankov, R., Becker, W.,& Gavrović-Jankulović, M.. (2008). A recombinant kiwi cystatin is a novel reagent for evaluation of the clinical relevance on phytocystatins in kiwi fruit allergy. in Allergy
Blackwell Publishing, Oxford., 63, 573-573.
https://hdl.handle.net/21.15107/rcub_intor_255

Popović M, Burazer L, Atanasković-Marković M, Milovanović M, Ćirković-Veličković T, Petersen A, Jankov R, Becker W, Gavrović-Jankulović M. A recombinant kiwi cystatin is a novel reagent for evaluation of the clinical relevance on phytocystatins in kiwi fruit allergy. in Allergy. 2008;63:573-573.
https://hdl.handle.net/21.15107/rcub_intor_255 .

Popović, Milica, Burazer, Lidija, Atanasković-Marković, Marina, Milovanović, Mina, Ćirković-Veličković, Tanja, Petersen, Arnd, Jankov, Ratko, Becker, Wolf-Meinhard, Gavrović-Jankulović, Marija, "A recombinant kiwi cystatin is a novel reagent for evaluation of the clinical relevance on phytocystatins in kiwi fruit allergy" in Allergy, 63 (2008):573-573,
https://hdl.handle.net/21.15107/rcub_intor_255 .