Abughren, Mohamed

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  • Abughren, Mohamed (3)
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Author's Bibliography

Optimization of the heterologous expression of banana glucanase in Escherichia coli (vol 77, pg 43, 2012)

Abughren, Mohamed; Popović, Milica; Dimitrijević, Rajna; Burazer, Lidija; Grozdanović, Milica; Atanasković-Marković, Marina; Gavrović-Jankulović, Marija

(Srpsko hemijsko društvo, Beograd, 2012)

TY  - JOUR
AU  - Abughren, Mohamed
AU  - Popović, Milica
AU  - Dimitrijević, Rajna
AU  - Burazer, Lidija
AU  - Grozdanović, Milica
AU  - Atanasković-Marković, Marina
AU  - Gavrović-Jankulović, Marija
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/364
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Optimization of the heterologous expression of banana glucanase in Escherichia coli (vol 77, pg 43, 2012)
EP  - 258
IS  - 2
SP  - 257
VL  - 77
UR  - conv_284
ER  - 
@article{
author = "Abughren, Mohamed and Popović, Milica and Dimitrijević, Rajna and Burazer, Lidija and Grozdanović, Milica and Atanasković-Marković, Marina and Gavrović-Jankulović, Marija",
year = "2012",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Optimization of the heterologous expression of banana glucanase in Escherichia coli (vol 77, pg 43, 2012)",
pages = "258-257",
number = "2",
volume = "77",
url = "conv_284"
}
Abughren, M., Popović, M., Dimitrijević, R., Burazer, L., Grozdanović, M., Atanasković-Marković, M.,& Gavrović-Jankulović, M.. (2012). Optimization of the heterologous expression of banana glucanase in Escherichia coli (vol 77, pg 43, 2012). in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(2), 257-258.
conv_284
Abughren M, Popović M, Dimitrijević R, Burazer L, Grozdanović M, Atanasković-Marković M, Gavrović-Jankulović M. Optimization of the heterologous expression of banana glucanase in Escherichia coli (vol 77, pg 43, 2012). in Journal of the Serbian Chemical Society. 2012;77(2):257-258.
conv_284 .
Abughren, Mohamed, Popović, Milica, Dimitrijević, Rajna, Burazer, Lidija, Grozdanović, Milica, Atanasković-Marković, Marina, Gavrović-Jankulović, Marija, "Optimization of the heterologous expression of banana glucanase in Escherichia coli (vol 77, pg 43, 2012)" in Journal of the Serbian Chemical Society, 77, no. 2 (2012):257-258,
conv_284 .

Optimization of the heterologous expression of banana glucanase in Escherichia coli

Abughren, Mohamed; Popović, Milica; Dimitrijević, Rajna; Burazer, Lidija; Grozdanović, Milica; Atanasković-Marković, Marina; Gavrović-Jankulović, Marija

(Srpsko hemijsko društvo, Beograd, 2012)

TY  - JOUR
AU  - Abughren, Mohamed
AU  - Popović, Milica
AU  - Dimitrijević, Rajna
AU  - Burazer, Lidija
AU  - Grozdanović, Milica
AU  - Atanasković-Marković, Marina
AU  - Gavrović-Jankulović, Marija
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/350
AB  - For the heterologous production of a banana glucanase in Escherichia coli, its gene (GenBank GQ268963) was cloned into a pG EX-4T expression vector as a fusion protein with glutathione-S-transferase (GST). BL21 cells transformed with the GST-Mus a 5 con struct were employed for production of the protein induced by 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG). The conditions for protein expression were optimized by varying the temperature (25, 30 and 37°C) and duration of protein expression (3, 6 and 12 h). The level of protein production was analyzed by densitometry of the sodium dodecyl sulfate-polyacrylamide gel (SDS-PAG) after electrophoretic resolution of the respective cell lysates. The optimal protein expression for downstream processing was obtained after 12 h of cell growth at 25°C upon addition of IPTG. Recombinant GST-Mus a 5 purified by glutathione affinity chromatography revealed a molecular mass of a bout 60 kDa. The IgE and IgG reactivity of the rGST-Mus a 5 was confirmed by dot blot an analysis with sera of individual patients from subjects with banana allergy and polyclonal rabbit antibodies against banana extract, respectively. The purified recombinant glucanase is a potential candidate for banana allergy diagnosis.
AB  - Za potrebe proizvodnje u Escherichia coli gen glukanaze iz banane (GenBank GQ268963) je ukloniran u ekspresioni vektor pGEX-4T sa glutation-S-transferazom (GST). Proizvodnja ovog proteina u ćelijama je indukovana 1 mM izopropil-β-D-tiogalaktopiranozidom (IPTG). Uslovi za ekspresiju proteina su optimizovani variranjem temperature (25, 30 i 37°C) i dužine trajanja proteinske sinteze (3, 6 i 12 h). Nivo proizvodnje proteina je analiziran denzitometrijom SDS-PA gela nakon elektroforetskog razdvajanja ćelijskih lizata. Optimalna proizvodnja proteina za njegovo dalje procesovanje je dobijena gajenjem ćelija nakon dodatka IPTG na 25°C tokom 12 h. Rekombinantni GST-Mus a 5 prečišćen afinitetnom hromatografijom sa glutationom pokazuje molekulsku masu od 60 kDa. IgE i IgG reaktivnost izolovane glukanaze potvrđena je u 'dot blot' sa pojedinačnim serumima osoba alergičnih na bananu, i sa poliklonskim zečijim antitelima na ekstrakt banane, redom. Prečišćena rekombinantna glukanaza je potencijalan kandidat za dijagnozu alergije na bananu.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Optimization of the heterologous expression of banana glucanase in Escherichia coli
T1  - Optimizacija heterologe proizvodnje glukanaze iz banane u E. coli
EP  - 52
IS  - 1
SP  - 43
VL  - 77
DO  - 10.2298/JSC110309158A
UR  - conv_24
ER  - 
@article{
author = "Abughren, Mohamed and Popović, Milica and Dimitrijević, Rajna and Burazer, Lidija and Grozdanović, Milica and Atanasković-Marković, Marina and Gavrović-Jankulović, Marija",
year = "2012",
abstract = "For the heterologous production of a banana glucanase in Escherichia coli, its gene (GenBank GQ268963) was cloned into a pG EX-4T expression vector as a fusion protein with glutathione-S-transferase (GST). BL21 cells transformed with the GST-Mus a 5 con struct were employed for production of the protein induced by 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG). The conditions for protein expression were optimized by varying the temperature (25, 30 and 37°C) and duration of protein expression (3, 6 and 12 h). The level of protein production was analyzed by densitometry of the sodium dodecyl sulfate-polyacrylamide gel (SDS-PAG) after electrophoretic resolution of the respective cell lysates. The optimal protein expression for downstream processing was obtained after 12 h of cell growth at 25°C upon addition of IPTG. Recombinant GST-Mus a 5 purified by glutathione affinity chromatography revealed a molecular mass of a bout 60 kDa. The IgE and IgG reactivity of the rGST-Mus a 5 was confirmed by dot blot an analysis with sera of individual patients from subjects with banana allergy and polyclonal rabbit antibodies against banana extract, respectively. The purified recombinant glucanase is a potential candidate for banana allergy diagnosis., Za potrebe proizvodnje u Escherichia coli gen glukanaze iz banane (GenBank GQ268963) je ukloniran u ekspresioni vektor pGEX-4T sa glutation-S-transferazom (GST). Proizvodnja ovog proteina u ćelijama je indukovana 1 mM izopropil-β-D-tiogalaktopiranozidom (IPTG). Uslovi za ekspresiju proteina su optimizovani variranjem temperature (25, 30 i 37°C) i dužine trajanja proteinske sinteze (3, 6 i 12 h). Nivo proizvodnje proteina je analiziran denzitometrijom SDS-PA gela nakon elektroforetskog razdvajanja ćelijskih lizata. Optimalna proizvodnja proteina za njegovo dalje procesovanje je dobijena gajenjem ćelija nakon dodatka IPTG na 25°C tokom 12 h. Rekombinantni GST-Mus a 5 prečišćen afinitetnom hromatografijom sa glutationom pokazuje molekulsku masu od 60 kDa. IgE i IgG reaktivnost izolovane glukanaze potvrđena je u 'dot blot' sa pojedinačnim serumima osoba alergičnih na bananu, i sa poliklonskim zečijim antitelima na ekstrakt banane, redom. Prečišćena rekombinantna glukanaza je potencijalan kandidat za dijagnozu alergije na bananu.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Optimization of the heterologous expression of banana glucanase in Escherichia coli, Optimizacija heterologe proizvodnje glukanaze iz banane u E. coli",
pages = "52-43",
number = "1",
volume = "77",
doi = "10.2298/JSC110309158A",
url = "conv_24"
}
Abughren, M., Popović, M., Dimitrijević, R., Burazer, L., Grozdanović, M., Atanasković-Marković, M.,& Gavrović-Jankulović, M.. (2012). Optimization of the heterologous expression of banana glucanase in Escherichia coli. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(1), 43-52.
https://doi.org/10.2298/JSC110309158A
conv_24
Abughren M, Popović M, Dimitrijević R, Burazer L, Grozdanović M, Atanasković-Marković M, Gavrović-Jankulović M. Optimization of the heterologous expression of banana glucanase in Escherichia coli. in Journal of the Serbian Chemical Society. 2012;77(1):43-52.
doi:10.2298/JSC110309158A
conv_24 .
Abughren, Mohamed, Popović, Milica, Dimitrijević, Rajna, Burazer, Lidija, Grozdanović, Milica, Atanasković-Marković, Marina, Gavrović-Jankulović, Marija, "Optimization of the heterologous expression of banana glucanase in Escherichia coli" in Journal of the Serbian Chemical Society, 77, no. 1 (2012):43-52,
https://doi.org/10.2298/JSC110309158A .,
conv_24 .
1
2
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Erratum: Complementary-DNA (cDNA): (Journal of the Serbian Chemical Society (2012) 77:1 (257-258))

Abughren, Mohamed; Popović, Milica; Dimitrijević, Rajna; Burazer, Lidija; Grozdanović, Milica; Atanasković-Marković, Marina; Gavrović-Jankulović, Marija

(Srpsko hemijsko društvo, Beograd, 2012)

TY  - JOUR
AU  - Abughren, Mohamed
AU  - Popović, Milica
AU  - Dimitrijević, Rajna
AU  - Burazer, Lidija
AU  - Grozdanović, Milica
AU  - Atanasković-Marković, Marina
AU  - Gavrović-Jankulović, Marija
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/365
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Erratum: Complementary-DNA (cDNA): (Journal of the Serbian Chemical Society (2012) 77:1 (257-258))
EP  - 258
IS  - 2
SP  - 257
VL  - 77
UR  - conv_590
ER  - 
@article{
author = "Abughren, Mohamed and Popović, Milica and Dimitrijević, Rajna and Burazer, Lidija and Grozdanović, Milica and Atanasković-Marković, Marina and Gavrović-Jankulović, Marija",
year = "2012",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Erratum: Complementary-DNA (cDNA): (Journal of the Serbian Chemical Society (2012) 77:1 (257-258))",
pages = "258-257",
number = "2",
volume = "77",
url = "conv_590"
}
Abughren, M., Popović, M., Dimitrijević, R., Burazer, L., Grozdanović, M., Atanasković-Marković, M.,& Gavrović-Jankulović, M.. (2012). Erratum: Complementary-DNA (cDNA): (Journal of the Serbian Chemical Society (2012) 77:1 (257-258)). in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(2), 257-258.
conv_590
Abughren M, Popović M, Dimitrijević R, Burazer L, Grozdanović M, Atanasković-Marković M, Gavrović-Jankulović M. Erratum: Complementary-DNA (cDNA): (Journal of the Serbian Chemical Society (2012) 77:1 (257-258)). in Journal of the Serbian Chemical Society. 2012;77(2):257-258.
conv_590 .
Abughren, Mohamed, Popović, Milica, Dimitrijević, Rajna, Burazer, Lidija, Grozdanović, Milica, Atanasković-Marković, Marina, Gavrović-Jankulović, Marija, "Erratum: Complementary-DNA (cDNA): (Journal of the Serbian Chemical Society (2012) 77:1 (257-258))" in Journal of the Serbian Chemical Society, 77, no. 2 (2012):257-258,
conv_590 .