TY  - JOUR
AU  - Sekulić, M.
AU  - Lovren, M.
AU  - Milošević, V.
AU  - Micić, M.
AU  - Balint-Perić, L.
PY  - 1998
UR  - http://intor.torlakinstitut.com/handle/123456789/95
AB  - The structure and function of C cells of middle-aged female rats (14-months old) treated with estradiol dipropionate (EDP), calcium (Ca) or a combination of EDP+Ca were studied. A stereological method was used to determine the volume of calcitonin (CT)-immunoreactive C cells and their nuclei, and the relative volume density and mean number of the C cells per section were calculated. Serum levels of CT, osteocalcin, parathyroid hormone (PTH), and beta-estradiol were also measured. A significant decrease in body weight of the rats treated with EDP or EDP+Ca was observed. These treatments led to a significant decrease in cellular and nuclear volumes, relative volume density, and mean number of C cells per section, in comparison with the corresponding controls. A reduction of the serum level of CT, PTH, and osteocalcin was also recorded in EDP-and EDP+Ca-treated animals. No statistically significant differences between Ca- and vehicle-injected rats, with regard to all morphometric C cell parameters and biochemical values determined, were seen. However, a conspicuous degranulation of the C cells and decreased immunoreactivity for CT in the Ca-receiving group, which could be interpreted as the signs of increased activity of these cells, were noticed. This effect of Ca was also observed in rats injected with EDP and Ca in combination, when the inhibitory effect of EDP on C cell function was less noticeable than in the group treated with EDP alone.
PB  - Springer, New York
T2  - Histochemistry and Cell Biology
T1  - Thyroid C cells of middle-aged rats treated with estradiol or calcium
EP  - 262
IS  - 3
SP  - 257
VL  - 109
DO  - 10.1007/s004180050225
ER  - 

@article{
author = "Sekulić, M. and Lovren, M. and Milošević, V. and Micić, M. and Balint-Perić, L.",
year = "1998",
abstract = "The structure and function of C cells of middle-aged female rats (14-months old) treated with estradiol dipropionate (EDP), calcium (Ca) or a combination of EDP+Ca were studied. A stereological method was used to determine the volume of calcitonin (CT)-immunoreactive C cells and their nuclei, and the relative volume density and mean number of the C cells per section were calculated. Serum levels of CT, osteocalcin, parathyroid hormone (PTH), and beta-estradiol were also measured. A significant decrease in body weight of the rats treated with EDP or EDP+Ca was observed. These treatments led to a significant decrease in cellular and nuclear volumes, relative volume density, and mean number of C cells per section, in comparison with the corresponding controls. A reduction of the serum level of CT, PTH, and osteocalcin was also recorded in EDP-and EDP+Ca-treated animals. No statistically significant differences between Ca- and vehicle-injected rats, with regard to all morphometric C cell parameters and biochemical values determined, were seen. However, a conspicuous degranulation of the C cells and decreased immunoreactivity for CT in the Ca-receiving group, which could be interpreted as the signs of increased activity of these cells, were noticed. This effect of Ca was also observed in rats injected with EDP and Ca in combination, when the inhibitory effect of EDP on C cell function was less noticeable than in the group treated with EDP alone.",
publisher = "Springer, New York",
journal = "Histochemistry and Cell Biology",
title = "Thyroid C cells of middle-aged rats treated with estradiol or calcium",
pages = "262-257",
number = "3",
volume = "109",
doi = "10.1007/s004180050225"
}

Sekulić, M., Lovren, M., Milošević, V., Micić, M.,& Balint-Perić, L.. (1998). Thyroid C cells of middle-aged rats treated with estradiol or calcium. in Histochemistry and Cell Biology
Springer, New York., 109(3), 257-262.
https://doi.org/10.1007/s004180050225

Sekulić M, Lovren M, Milošević V, Micić M, Balint-Perić L. Thyroid C cells of middle-aged rats treated with estradiol or calcium. in Histochemistry and Cell Biology. 1998;109(3):257-262.
doi:10.1007/s004180050225 .

Sekulić, M., Lovren, M., Milošević, V., Micić, M., Balint-Perić, L., "Thyroid C cells of middle-aged rats treated with estradiol or calcium" in Histochemistry and Cell Biology, 109, no. 3 (1998):257-262,
https://doi.org/10.1007/s004180050225 . .

TY  - JOUR
AU  - Lovren, M.
AU  - Sekulić, M.
AU  - Milošević, V.
AU  - Micić, M.
AU  - Radulović, N.
PY  - 1997
UR  - http://intor.torlakinstitut.com/handle/123456789/79
AB  - Immunocytochemical and morphometric changes of pituitary FSH and LH cells in juvenile (17th day of life) female rats neonatally (4th day of life) treated with five 0.25 mg doses of estradiol dipropionate (EDP) or human chorionic gonadotrophin (14th and 16th day of life) and with the combination of both were studied using rabbit antirat beta-follicle stimulating hormone (FSH) and beta-luteinising hormone (LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were applied to evaluate the changes in FSH- and LH-producing cells, number and volume densities of the cells and the nuclei. In EDP-treated females significantly increased pituitary mass and the number of chromophobes and PRL cells were observed while all morphometric parameters of FSH/LH cells were decreased in comparison with the corresponding controls. This difference was much more prominent in FSH-beta- than in LH-beta cells. Treatment with hCG led to an increase in the number of FSH and LH cells, but it was much more pronounced in the latter cells. In the group receiving EDP and hCG in combination, the effect of EDP was predominant in FSH cells and that of hCG in LH cells.
PB  - Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Jugoslovenska Medicinska Biohemija - Yugoslav Medical Biochemistry
T1  - Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment
EP  - 87
IS  - 2
SP  - 83
VL  - 16
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1877
ER  - 

@article{
author = "Lovren, M. and Sekulić, M. and Milošević, V. and Micić, M. and Radulović, N.",
year = "1997",
abstract = "Immunocytochemical and morphometric changes of pituitary FSH and LH cells in juvenile (17th day of life) female rats neonatally (4th day of life) treated with five 0.25 mg doses of estradiol dipropionate (EDP) or human chorionic gonadotrophin (14th and 16th day of life) and with the combination of both were studied using rabbit antirat beta-follicle stimulating hormone (FSH) and beta-luteinising hormone (LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Morphometry and stereology were applied to evaluate the changes in FSH- and LH-producing cells, number and volume densities of the cells and the nuclei. In EDP-treated females significantly increased pituitary mass and the number of chromophobes and PRL cells were observed while all morphometric parameters of FSH/LH cells were decreased in comparison with the corresponding controls. This difference was much more prominent in FSH-beta- than in LH-beta cells. Treatment with hCG led to an increase in the number of FSH and LH cells, but it was much more pronounced in the latter cells. In the group receiving EDP and hCG in combination, the effect of EDP was predominant in FSH cells and that of hCG in LH cells.",
publisher = "Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Jugoslovenska Medicinska Biohemija - Yugoslav Medical Biochemistry",
title = "Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment",
pages = "87-83",
number = "2",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1877"
}

Lovren, M., Sekulić, M., Milošević, V., Micić, M.,& Radulović, N.. (1997). Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment. in Jugoslovenska Medicinska Biohemija - Yugoslav Medical Biochemistry
Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd., 16(2), 83-87.
https://hdl.handle.net/21.15107/rcub_ibiss_1877

Lovren M, Sekulić M, Milošević V, Micić M, Radulović N. Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment. in Jugoslovenska Medicinska Biohemija - Yugoslav Medical Biochemistry. 1997;16(2):83-87.
https://hdl.handle.net/21.15107/rcub_ibiss_1877 .

Lovren, M., Sekulić, M., Milošević, V., Micić, M., Radulović, N., "Alterations of pituitary immunocytochemical and morphometric parameters in juvenile female rats following estradiol and hCG treatment" in Jugoslovenska Medicinska Biohemija - Yugoslav Medical Biochemistry, 16, no. 2 (1997):83-87,
https://hdl.handle.net/21.15107/rcub_ibiss_1877 .