Vojinović, Dragica

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orcid::0000-0002-4890-3239
  • Vojinović, Dragica (5)
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Supplementary information for the article: Vasić, A.; Răileanu, C.; Körsten, C.; Vojinović, D.; Manić, M.; Urošević, A.; Nikolić, N.; Dulović, O.; Tews, B. A.; Petrović, T.; Silaghi, C.; Valčić, M.; Gligić, A. West Nile Virus in the Republic of Serbia—Diagnostic Performance of Five Serological Tests in Dog and Horse Sera. Transboundary and Emerging Diseases 2022, 69 (5), e2506–e2515. https://doi.org/10.1111/tbed.14593.

Vasić, Ana; Răileanu, Cristian; Körsten, Christin; Vojinović, Dragica; Manić, Marija; Urošević, Aleksandar; Nikolić, Nataša; Dulović, Olga; Tews, Birke Andrea; Petrović, Tamaš; Silaghi, Cornelia; Valčić, Miroslav; Gligić, Ana

(2022)

TY  - DATA
AU  - Vasić, Ana
AU  - Răileanu, Cristian
AU  - Körsten, Christin
AU  - Vojinović, Dragica
AU  - Manić, Marija
AU  - Urošević, Aleksandar
AU  - Nikolić, Nataša
AU  - Dulović, Olga
AU  - Tews, Birke Andrea
AU  - Petrović, Tamaš
AU  - Silaghi, Cornelia
AU  - Valčić, Miroslav
AU  - Gligić, Ana
PY  - 2022
UR  - http://intor.torlakinstitut.com/handle/123456789/648
AB  - Supplementary file 1.  Dog and horse sera sampled from 15 locations from 2011 to 2013 for the serological study. Supplementary file 2.  Seropositivity in dogs and horses in Serbia in period 2011-2013. Supplementary file 3.  The results of „in-house“ and commercial ELISA tests in dog sera. Supplementary file 4.  The results of „in-house“ and commercial IFA and ELISA tests in horse  sera
T2  - Transboundary and Emerging Diseases
T1  - Supplementary information for the article: Vasić, A.; Răileanu, C.; Körsten, C.; Vojinović, D.; Manić, M.; Urošević,  A.; Nikolić, N.; Dulović, O.; Tews, B. A.; Petrović, T.; Silaghi, C.; Valčić, M.; Gligić, A. West Nile Virus in the Republic  of Serbia—Diagnostic Performance of Five Serological Tests in Dog and Horse Sera. Transboundary and Emerging Diseases 2022,  69 (5), e2506–e2515. https://doi.org/10.1111/tbed.14593.
EP  - e2515
IS  - 5
SP  - e2506
VL  - 69
UR  - https://hdl.handle.net/21.15107/rcub_intor_648
ER  - 
@misc{
author = "Vasić, Ana and Răileanu, Cristian and Körsten, Christin and Vojinović, Dragica and Manić, Marija and Urošević, Aleksandar and Nikolić, Nataša and Dulović, Olga and Tews, Birke Andrea and Petrović, Tamaš and Silaghi, Cornelia and Valčić, Miroslav and Gligić, Ana",
year = "2022",
abstract = "Supplementary file 1.  Dog and horse sera sampled from 15 locations from 2011 to 2013 for the serological study. Supplementary file 2.  Seropositivity in dogs and horses in Serbia in period 2011-2013. Supplementary file 3.  The results of „in-house“ and commercial ELISA tests in dog sera. Supplementary file 4.  The results of „in-house“ and commercial IFA and ELISA tests in horse  sera",
journal = "Transboundary and Emerging Diseases",
title = "Supplementary information for the article: Vasić, A.; Răileanu, C.; Körsten, C.; Vojinović, D.; Manić, M.; Urošević,  A.; Nikolić, N.; Dulović, O.; Tews, B. A.; Petrović, T.; Silaghi, C.; Valčić, M.; Gligić, A. West Nile Virus in the Republic  of Serbia—Diagnostic Performance of Five Serological Tests in Dog and Horse Sera. Transboundary and Emerging Diseases 2022,  69 (5), e2506–e2515. https://doi.org/10.1111/tbed.14593.",
pages = "e2515-e2506",
number = "5",
volume = "69",
url = "https://hdl.handle.net/21.15107/rcub_intor_648"
}
Vasić, A., Răileanu, C., Körsten, C., Vojinović, D., Manić, M., Urošević, A., Nikolić, N., Dulović, O., Tews, B. A., Petrović, T., Silaghi, C., Valčić, M.,& Gligić, A.. (2022). Supplementary information for the article: Vasić, A.; Răileanu, C.; Körsten, C.; Vojinović, D.; Manić, M.; Urošević,  A.; Nikolić, N.; Dulović, O.; Tews, B. A.; Petrović, T.; Silaghi, C.; Valčić, M.; Gligić, A. West Nile Virus in the Republic  of Serbia—Diagnostic Performance of Five Serological Tests in Dog and Horse Sera. Transboundary and Emerging Diseases 2022,  69 (5), e2506–e2515. https://doi.org/10.1111/tbed.14593.. in Transboundary and Emerging Diseases, 69(5), e2506-e2515.
https://hdl.handle.net/21.15107/rcub_intor_648
Vasić A, Răileanu C, Körsten C, Vojinović D, Manić M, Urošević A, Nikolić N, Dulović O, Tews BA, Petrović T, Silaghi C, Valčić M, Gligić A. Supplementary information for the article: Vasić, A.; Răileanu, C.; Körsten, C.; Vojinović, D.; Manić, M.; Urošević,  A.; Nikolić, N.; Dulović, O.; Tews, B. A.; Petrović, T.; Silaghi, C.; Valčić, M.; Gligić, A. West Nile Virus in the Republic  of Serbia—Diagnostic Performance of Five Serological Tests in Dog and Horse Sera. Transboundary and Emerging Diseases 2022,  69 (5), e2506–e2515. https://doi.org/10.1111/tbed.14593.. in Transboundary and Emerging Diseases. 2022;69(5):e2506-e2515.
https://hdl.handle.net/21.15107/rcub_intor_648 .
Vasić, Ana, Răileanu, Cristian, Körsten, Christin, Vojinović, Dragica, Manić, Marija, Urošević, Aleksandar, Nikolić, Nataša, Dulović, Olga, Tews, Birke Andrea, Petrović, Tamaš, Silaghi, Cornelia, Valčić, Miroslav, Gligić, Ana, "Supplementary information for the article: Vasić, A.; Răileanu, C.; Körsten, C.; Vojinović, D.; Manić, M.; Urošević,  A.; Nikolić, N.; Dulović, O.; Tews, B. A.; Petrović, T.; Silaghi, C.; Valčić, M.; Gligić, A. West Nile Virus in the Republic  of Serbia—Diagnostic Performance of Five Serological Tests in Dog and Horse Sera. Transboundary and Emerging Diseases 2022,  69 (5), e2506–e2515. https://doi.org/10.1111/tbed.14593." in Transboundary and Emerging Diseases, 69, no. 5 (2022):e2506-e2515,
https://hdl.handle.net/21.15107/rcub_intor_648 .

West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera

Vasić, Ana; Răileanu, Cristian; Körsten, Christin; Vojinović, Dragica; Manić, Marija; Urošević, Aleksandar; Nikolić, Nataša; Dulović, Olga; Tews, Birke Andrea; Petrović, Tamaš; Silaghi, Cornelia; Valčić, Miroslav; Gligić, Ana

(Wiley, 2022)

TY  - JOUR
AU  - Vasić, Ana
AU  - Răileanu, Cristian
AU  - Körsten, Christin
AU  - Vojinović, Dragica
AU  - Manić, Marija
AU  - Urošević, Aleksandar
AU  - Nikolić, Nataša
AU  - Dulović, Olga
AU  - Tews, Birke Andrea
AU  - Petrović, Tamaš
AU  - Silaghi, Cornelia
AU  - Valčić, Miroslav
AU  - Gligić, Ana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1238
UR  - http://intor.torlakinstitut.com/handle/123456789/623
AB  - West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.
PB  - Wiley
T2  - Transboundary and Emerging Diseases
T1  - West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera
EP  - e2515
IS  - 5
SP  - e2506
VL  - 69
DO  - 10.1111/tbed.14593
ER  - 
@article{
author = "Vasić, Ana and Răileanu, Cristian and Körsten, Christin and Vojinović, Dragica and Manić, Marija and Urošević, Aleksandar and Nikolić, Nataša and Dulović, Olga and Tews, Birke Andrea and Petrović, Tamaš and Silaghi, Cornelia and Valčić, Miroslav and Gligić, Ana",
year = "2022",
abstract = "West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.",
publisher = "Wiley",
journal = "Transboundary and Emerging Diseases",
title = "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera",
pages = "e2515-e2506",
number = "5",
volume = "69",
doi = "10.1111/tbed.14593"
}
Vasić, A., Răileanu, C., Körsten, C., Vojinović, D., Manić, M., Urošević, A., Nikolić, N., Dulović, O., Tews, B. A., Petrović, T., Silaghi, C., Valčić, M.,& Gligić, A.. (2022). West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases
Wiley., 69(5), e2506-e2515.
https://doi.org/10.1111/tbed.14593
Vasić A, Răileanu C, Körsten C, Vojinović D, Manić M, Urošević A, Nikolić N, Dulović O, Tews BA, Petrović T, Silaghi C, Valčić M, Gligić A. West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera. in Transboundary and Emerging Diseases. 2022;69(5):e2506-e2515.
doi:10.1111/tbed.14593 .
Vasić, Ana, Răileanu, Cristian, Körsten, Christin, Vojinović, Dragica, Manić, Marija, Urošević, Aleksandar, Nikolić, Nataša, Dulović, Olga, Tews, Birke Andrea, Petrović, Tamaš, Silaghi, Cornelia, Valčić, Miroslav, Gligić, Ana, "West Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera" in Transboundary and Emerging Diseases, 69, no. 5 (2022):e2506-e2515,
https://doi.org/10.1111/tbed.14593 . .
2
1
1

Seroprevalence of ehrlichia canis infection in stray dogs from Serbia

Bogićević, Nataša; Elezović-Radovanović, Milica; Vasić, Ana; Manić, Marija; Marić, Jovan; Vojinović, Dragica; Rogožarski, Dragan; Gligić, Ana; Valčić, Miroslav

(Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje, 2017)

TY  - JOUR
AU  - Bogićević, Nataša
AU  - Elezović-Radovanović, Milica
AU  - Vasić, Ana
AU  - Manić, Marija
AU  - Marić, Jovan
AU  - Vojinović, Dragica
AU  - Rogožarski, Dragan
AU  - Gligić, Ana
AU  - Valčić, Miroslav
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/494
AB  - Canine Monocytic Ehrlichiosis is a zoonotic bacterial disease with worldwide distribution. With regards to the population of stray dogs, the disease is facilitated due to their lifestyle and the lack of anti-parasitic protection. The aim of this study was to provide serological data on the presence of a specific Ehrlichia canis IgG antibodies in stray dogs, originating from 7 municipalities in Serbia. During the period from April 2013 to June 2014, 217 canine sera were submitted to the laboratory of the Department of Infectious Diseases of Animals and Bees, Faculty of Veterinary Medicine in Belgrade. An immunofluorescent antibody test (IFAT) was performed to detect antibodies to Ehrlichia canis (cut off, 1: 50). Seropositive dogs were found in 5 out of 7 counties with a seroprevalence varying from 3.57% to 20% and an overall seroprevalence of 11.06% (24/217). There was no statistically significant difference between the prevalence of infection and the host age or gender. Results showed that stray dogs contribute to maintaining and spreading of Ehrlichia canis in Serbia. Due to the close relationship between people and dogs, it is of great importance to constantly monitor and improve prevention of this disease.
PB  - Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje
T2  - Macedonian Veterinary Review
T1  - Seroprevalence of ehrlichia canis infection in stray dogs from Serbia
EP  - 42
IS  - 1
SP  - 37
VL  - 40
DO  - 10.1515/macvetrev-2016-0096
ER  - 
@article{
author = "Bogićević, Nataša and Elezović-Radovanović, Milica and Vasić, Ana and Manić, Marija and Marić, Jovan and Vojinović, Dragica and Rogožarski, Dragan and Gligić, Ana and Valčić, Miroslav",
year = "2017",
abstract = "Canine Monocytic Ehrlichiosis is a zoonotic bacterial disease with worldwide distribution. With regards to the population of stray dogs, the disease is facilitated due to their lifestyle and the lack of anti-parasitic protection. The aim of this study was to provide serological data on the presence of a specific Ehrlichia canis IgG antibodies in stray dogs, originating from 7 municipalities in Serbia. During the period from April 2013 to June 2014, 217 canine sera were submitted to the laboratory of the Department of Infectious Diseases of Animals and Bees, Faculty of Veterinary Medicine in Belgrade. An immunofluorescent antibody test (IFAT) was performed to detect antibodies to Ehrlichia canis (cut off, 1: 50). Seropositive dogs were found in 5 out of 7 counties with a seroprevalence varying from 3.57% to 20% and an overall seroprevalence of 11.06% (24/217). There was no statistically significant difference between the prevalence of infection and the host age or gender. Results showed that stray dogs contribute to maintaining and spreading of Ehrlichia canis in Serbia. Due to the close relationship between people and dogs, it is of great importance to constantly monitor and improve prevention of this disease.",
publisher = "Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje",
journal = "Macedonian Veterinary Review",
title = "Seroprevalence of ehrlichia canis infection in stray dogs from Serbia",
pages = "42-37",
number = "1",
volume = "40",
doi = "10.1515/macvetrev-2016-0096"
}
Bogićević, N., Elezović-Radovanović, M., Vasić, A., Manić, M., Marić, J., Vojinović, D., Rogožarski, D., Gligić, A.,& Valčić, M.. (2017). Seroprevalence of ehrlichia canis infection in stray dogs from Serbia. in Macedonian Veterinary Review
Univ Sv Kiril & Metodij Skopje, Fak Veterinarna Medicina, Skopje., 40(1), 37-42.
https://doi.org/10.1515/macvetrev-2016-0096
Bogićević N, Elezović-Radovanović M, Vasić A, Manić M, Marić J, Vojinović D, Rogožarski D, Gligić A, Valčić M. Seroprevalence of ehrlichia canis infection in stray dogs from Serbia. in Macedonian Veterinary Review. 2017;40(1):37-42.
doi:10.1515/macvetrev-2016-0096 .
Bogićević, Nataša, Elezović-Radovanović, Milica, Vasić, Ana, Manić, Marija, Marić, Jovan, Vojinović, Dragica, Rogožarski, Dragan, Gligić, Ana, Valčić, Miroslav, "Seroprevalence of ehrlichia canis infection in stray dogs from Serbia" in Macedonian Veterinary Review, 40, no. 1 (2017):37-42,
https://doi.org/10.1515/macvetrev-2016-0096 . .
12
4
8

Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia

Đuričić, Bosiljka; Vasić, Ana; Rogožarski, Dragan; Vojinović, Dragica; Elezović-Radovanović, Milica; Manić, Marija; Marić, Jovan; Prokić, Nataša; Ilić, Živka; Novotny, N.; Gligić, Ana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2013)

TY  - JOUR
AU  - Đuričić, Bosiljka
AU  - Vasić, Ana
AU  - Rogožarski, Dragan
AU  - Vojinović, Dragica
AU  - Elezović-Radovanović, Milica
AU  - Manić, Marija
AU  - Marić, Jovan
AU  - Prokić, Nataša
AU  - Ilić, Živka
AU  - Novotny, N.
AU  - Gligić, Ana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/366
AB  - The disease caused by the West Nile virus (WNV) has been known since 1937 when it was described for the first time in Uganda. After spreading to Europe and the Middle East, the disease has changed its primary location. Today WN infection is a significant health problem in the world. As a result of the current epizootiological and epidemiological situation in Europe studies on the occurrence of WND were introduced in Serbia, also. The investigation on the presence of WNV antibodies was intensified in the period from 2008 to 2012. In this period a total of 3618 serum samples were tested from 18 localities (2736 animal sera from 8 different species and 882 human sera samples). The method applied was gel immunodiffusion and the representative samples were confirmed by the plaque reduction test (PRNT-90). Out of the total number of samples WNV antibodies were present in 3.97% horses, 0.93% dogs, 0.31% poultry and 1.36% man. In one horse serum sample there was a positive reaction with a positive control serum, thus indicating the presence of WNV antigen. The results have confirmed that WNV antibodies are present in 9 out of 18 tested locations in the Republic of Serbia. The percentage of seropositive samples varies from 0.42% in Pozarevac (horses and humans) up to 6.45% in Novi Pazar (dogs). Out of the investigated species the highest seropositivity was recorded in horses (3.97%), and lowest in poultry (0.31%). WNV is present and widespread in the Republic of Serbia, thus enabling distribution mapping.
AB  - Bolest koju izaziva virus Zapadnog Nila (WNV) je poznata još od 1937. kada je po prvi put opisana u Ugandi. Posle širenja virusa u Evropu i na Bliski istok, bolest je promenila prvobitnu lokaciju. Danas je WN infekcija značajan zdravstveni problem u svetu. Kao rezultat trenutne epizootiološko-epidemiološke situacije u Evropi, uvedena su istraživanja prisustva WNV i u Srbiji. Istraživanja prisustva antitela za WNV intenzivirana su u periodu od 2008. do 2012. godine. U ovom periodu ukupno je ispitano 3618 seruma sa 18 lokaliteta (2736 seruma životinja, od 8 različitih vrsta i 882 seruma ljudi). Korišćen je metod imunodifuzije u gelu a reprezentativni uzorak potvrđen je testom neutralizacije plaka (PRNT- 90). Od ukupnog broja uzoraka na prisustvo antitela za WNV bilo je pozitivno 3,97% konja, 0,93% pasa, 0,31% domaće živine i 1,36% ljudi. U serumu jednog konja pojavila se pozitivna reakcija sa pozitivnim kontrolnim serumom, ukazujući na prisustvo antigena WNV. Dobijeni rezultati su potvrdili da su antitela za WNV prisutna u 9 od 18 ispitivanih lokacija u Republici Srbiji. Procenat seropozitivnih varira od 0.42% u Požarevcu (konji i ljudi) do 6,45% u Novom Pazaru (psi). Od ispitivanih vrsta najveća seropozitivnost je registrovana kod konja (3,97%), a najniža kod živine (0,31%). Raspoloživi podaci ukazuju da je WNV prisutan i značajno raširen u Republici Srbiji, što je omogućilo i mapiranje njegovog širenja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia
T1  - Seroepizootiološko-epidemiološka istraživanja i mapiranje infekcije Zapadnog Nila u Republici Srbiji
EP  - 579
IS  - 5-6
SP  - 569
VL  - 63
DO  - 10.2298/AVB1306569D
ER  - 
@article{
author = "Đuričić, Bosiljka and Vasić, Ana and Rogožarski, Dragan and Vojinović, Dragica and Elezović-Radovanović, Milica and Manić, Marija and Marić, Jovan and Prokić, Nataša and Ilić, Živka and Novotny, N. and Gligić, Ana",
year = "2013",
abstract = "The disease caused by the West Nile virus (WNV) has been known since 1937 when it was described for the first time in Uganda. After spreading to Europe and the Middle East, the disease has changed its primary location. Today WN infection is a significant health problem in the world. As a result of the current epizootiological and epidemiological situation in Europe studies on the occurrence of WND were introduced in Serbia, also. The investigation on the presence of WNV antibodies was intensified in the period from 2008 to 2012. In this period a total of 3618 serum samples were tested from 18 localities (2736 animal sera from 8 different species and 882 human sera samples). The method applied was gel immunodiffusion and the representative samples were confirmed by the plaque reduction test (PRNT-90). Out of the total number of samples WNV antibodies were present in 3.97% horses, 0.93% dogs, 0.31% poultry and 1.36% man. In one horse serum sample there was a positive reaction with a positive control serum, thus indicating the presence of WNV antigen. The results have confirmed that WNV antibodies are present in 9 out of 18 tested locations in the Republic of Serbia. The percentage of seropositive samples varies from 0.42% in Pozarevac (horses and humans) up to 6.45% in Novi Pazar (dogs). Out of the investigated species the highest seropositivity was recorded in horses (3.97%), and lowest in poultry (0.31%). WNV is present and widespread in the Republic of Serbia, thus enabling distribution mapping., Bolest koju izaziva virus Zapadnog Nila (WNV) je poznata još od 1937. kada je po prvi put opisana u Ugandi. Posle širenja virusa u Evropu i na Bliski istok, bolest je promenila prvobitnu lokaciju. Danas je WN infekcija značajan zdravstveni problem u svetu. Kao rezultat trenutne epizootiološko-epidemiološke situacije u Evropi, uvedena su istraživanja prisustva WNV i u Srbiji. Istraživanja prisustva antitela za WNV intenzivirana su u periodu od 2008. do 2012. godine. U ovom periodu ukupno je ispitano 3618 seruma sa 18 lokaliteta (2736 seruma životinja, od 8 različitih vrsta i 882 seruma ljudi). Korišćen je metod imunodifuzije u gelu a reprezentativni uzorak potvrđen je testom neutralizacije plaka (PRNT- 90). Od ukupnog broja uzoraka na prisustvo antitela za WNV bilo je pozitivno 3,97% konja, 0,93% pasa, 0,31% domaće živine i 1,36% ljudi. U serumu jednog konja pojavila se pozitivna reakcija sa pozitivnim kontrolnim serumom, ukazujući na prisustvo antigena WNV. Dobijeni rezultati su potvrdili da su antitela za WNV prisutna u 9 od 18 ispitivanih lokacija u Republici Srbiji. Procenat seropozitivnih varira od 0.42% u Požarevcu (konji i ljudi) do 6,45% u Novom Pazaru (psi). Od ispitivanih vrsta najveća seropozitivnost je registrovana kod konja (3,97%), a najniža kod živine (0,31%). Raspoloživi podaci ukazuju da je WNV prisutan i značajno raširen u Republici Srbiji, što je omogućilo i mapiranje njegovog širenja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia, Seroepizootiološko-epidemiološka istraživanja i mapiranje infekcije Zapadnog Nila u Republici Srbiji",
pages = "579-569",
number = "5-6",
volume = "63",
doi = "10.2298/AVB1306569D"
}
Đuričić, B., Vasić, A., Rogožarski, D., Vojinović, D., Elezović-Radovanović, M., Manić, M., Marić, J., Prokić, N., Ilić, Ž., Novotny, N.,& Gligić, A.. (2013). Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 569-579.
https://doi.org/10.2298/AVB1306569D
Đuričić B, Vasić A, Rogožarski D, Vojinović D, Elezović-Radovanović M, Manić M, Marić J, Prokić N, Ilić Ž, Novotny N, Gligić A. Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia. in Acta veterinaria - Beograd. 2013;63(5-6):569-579.
doi:10.2298/AVB1306569D .
Đuričić, Bosiljka, Vasić, Ana, Rogožarski, Dragan, Vojinović, Dragica, Elezović-Radovanović, Milica, Manić, Marija, Marić, Jovan, Prokić, Nataša, Ilić, Živka, Novotny, N., Gligić, Ana, "Seroepizootiological-epidemiological investigation and mapping of West Nile infection in the Republic of Serbia" in Acta veterinaria - Beograd, 63, no. 5-6 (2013):569-579,
https://doi.org/10.2298/AVB1306569D . .
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Review of new multicausal chronical poultry disease

Miljković, Biljana; Spalević, Ljiljana; Vojinović, Dragica; Pavlović, Ivan; Mićić, Mileva; Živković, Radmila

(Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad, 2002)

TY  - JOUR
AU  - Miljković, Biljana
AU  - Spalević, Ljiljana
AU  - Vojinović, Dragica
AU  - Pavlović, Ivan
AU  - Mićić, Mileva
AU  - Živković, Radmila
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/144
AB  - Aim of our work was to review our examination of new chronically poultry disease occurred at parent flocks, at 4 different epizootiological area. Disease was registries only at one poultry provinience (Hybro-G) between 27-28 weeks of old when they had 40-60% of laying capacity. Clinical signs of disease was same at all flocks: pale coulored feety like diarrhea with or without urats loos of feders at beck, near cloaca and at abdomen with characteristic skin inflammation. Because loss so much water via faeces in objects of breeding we felt a characteristic odur smell of diarrhea and increased oh humidity. From same reason flocks look like older birds. Parallel with this signs we occurred rupture of skin at lateral part of the one side of body, near cloaca predominately in female. During observation of poultry flocks at 2 epizootiological area we sow fever and hypersensitivity during catching to used blood samples and clacal swabs. Skin rupture was not occurred at all flocks. At same time we had decreased of production results but we not examined same. Died and sacrified birds we examined by patoanatomic examination when we did not see any pathological changes. Using by immunoflorescent tests we occurred Brachyspira spp.
AB  - Opis pojave novog hroničnog obolenja živine, u kategoriji brojlerskih roditelja & eksploataciji na četiri različita epizootiološka područja. Pojava je uočena kod samo jedne provenience (Hybro-C) sa početkom u 27-28. nedelji, a pri nosivosti od 40-60%. Klinička zapažanja su bila sledeća: proliv specifičnog mirisa, svetlije boje, masnog i vodenog penušavog izgleda sa ili bez urata, gubitak perja u predelu leđa oko kloake i u predelu trbuha sa karakterističnom upaljenom kožom. Zbog velike količine vode koje jedinke izluče fecesom objekat sa prisutnim jedinkama ima specifičan miris a vlažan mikroklimat. Zbog dehidratacije jato poprima izgled starije kateogrije. Uz navedene simptome zapaženo je i jednostrano pucanje koze predominantno ispoljeno kod kokica. Opserviranjem živih jedinki sa dva epizootiološka područja uočeno je febrilno stanje i hipersenzitivnost na zahvate hvatanja prilikom uzimanja krvi i kloakalnih briseva. Pucanje kože nije bilo ispoljeno kod svih ispitanih jata. Proizvodni rezultati su se razlikovali ali oni nisu bill predmet naših ispitivanja. Uginule i žrtvovane kokice i petlovi pri patoanatomskom pregledu nisu imali promene na unutrašnjim organima. Imunofluorescentnim testom koji nije rađen u našoj laboratoriji ustanovljeno je prisustvo Brachyspire sp. Sveži razmazi krvi i kloakalnih briseva analizovani su mikroskopskim pregledom u tamnom polju. Napravljeni su nativni preparati od svežih razmaza organa i obojeni May-Grunwald-Gimza bojom i analizovani su svetlosnom mikroskopijom. Rezultati naših ispitivanja ukazali su na prisustvo filarodinih morfoloških struktura u testisima, krvi iz predela srca i periferne krvi bubrega, jetre, kože oko kloake a retko u slezini, plućima i oku. Primenom Knott's metode u pregledanoj krvi nađena je struktura koja je slična nematodama. Naš nalaz sugeriše da se radi o dvojnoj infekciji što zahteva dodatno ispitivanje uz identifikaciju uzročnika radi iznalaženja rešenja suzbijanja i preveniranja ovog obolenja.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Review of new multicausal chronical poultry disease
T1  - Prikaz novog multikauzalnog hroničnog obolenja živine
EP  - 308
IS  - 3-4
SP  - 301
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_intor_144
ER  - 
@article{
author = "Miljković, Biljana and Spalević, Ljiljana and Vojinović, Dragica and Pavlović, Ivan and Mićić, Mileva and Živković, Radmila",
year = "2002",
abstract = "Aim of our work was to review our examination of new chronically poultry disease occurred at parent flocks, at 4 different epizootiological area. Disease was registries only at one poultry provinience (Hybro-G) between 27-28 weeks of old when they had 40-60% of laying capacity. Clinical signs of disease was same at all flocks: pale coulored feety like diarrhea with or without urats loos of feders at beck, near cloaca and at abdomen with characteristic skin inflammation. Because loss so much water via faeces in objects of breeding we felt a characteristic odur smell of diarrhea and increased oh humidity. From same reason flocks look like older birds. Parallel with this signs we occurred rupture of skin at lateral part of the one side of body, near cloaca predominately in female. During observation of poultry flocks at 2 epizootiological area we sow fever and hypersensitivity during catching to used blood samples and clacal swabs. Skin rupture was not occurred at all flocks. At same time we had decreased of production results but we not examined same. Died and sacrified birds we examined by patoanatomic examination when we did not see any pathological changes. Using by immunoflorescent tests we occurred Brachyspira spp., Opis pojave novog hroničnog obolenja živine, u kategoriji brojlerskih roditelja & eksploataciji na četiri različita epizootiološka područja. Pojava je uočena kod samo jedne provenience (Hybro-C) sa početkom u 27-28. nedelji, a pri nosivosti od 40-60%. Klinička zapažanja su bila sledeća: proliv specifičnog mirisa, svetlije boje, masnog i vodenog penušavog izgleda sa ili bez urata, gubitak perja u predelu leđa oko kloake i u predelu trbuha sa karakterističnom upaljenom kožom. Zbog velike količine vode koje jedinke izluče fecesom objekat sa prisutnim jedinkama ima specifičan miris a vlažan mikroklimat. Zbog dehidratacije jato poprima izgled starije kateogrije. Uz navedene simptome zapaženo je i jednostrano pucanje koze predominantno ispoljeno kod kokica. Opserviranjem živih jedinki sa dva epizootiološka područja uočeno je febrilno stanje i hipersenzitivnost na zahvate hvatanja prilikom uzimanja krvi i kloakalnih briseva. Pucanje kože nije bilo ispoljeno kod svih ispitanih jata. Proizvodni rezultati su se razlikovali ali oni nisu bill predmet naših ispitivanja. Uginule i žrtvovane kokice i petlovi pri patoanatomskom pregledu nisu imali promene na unutrašnjim organima. Imunofluorescentnim testom koji nije rađen u našoj laboratoriji ustanovljeno je prisustvo Brachyspire sp. Sveži razmazi krvi i kloakalnih briseva analizovani su mikroskopskim pregledom u tamnom polju. Napravljeni su nativni preparati od svežih razmaza organa i obojeni May-Grunwald-Gimza bojom i analizovani su svetlosnom mikroskopijom. Rezultati naših ispitivanja ukazali su na prisustvo filarodinih morfoloških struktura u testisima, krvi iz predela srca i periferne krvi bubrega, jetre, kože oko kloake a retko u slezini, plućima i oku. Primenom Knott's metode u pregledanoj krvi nađena je struktura koja je slična nematodama. Naš nalaz sugeriše da se radi o dvojnoj infekciji što zahteva dodatno ispitivanje uz identifikaciju uzročnika radi iznalaženja rešenja suzbijanja i preveniranja ovog obolenja.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Review of new multicausal chronical poultry disease, Prikaz novog multikauzalnog hroničnog obolenja živine",
pages = "308-301",
number = "3-4",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_intor_144"
}
Miljković, B., Spalević, L., Vojinović, D., Pavlović, I., Mićić, M.,& Živković, R.. (2002). Review of new multicausal chronical poultry disease. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 51(3-4), 301-308.
https://hdl.handle.net/21.15107/rcub_intor_144
Miljković B, Spalević L, Vojinović D, Pavlović I, Mićić M, Živković R. Review of new multicausal chronical poultry disease. in Savremena poljoprivreda. 2002;51(3-4):301-308.
https://hdl.handle.net/21.15107/rcub_intor_144 .
Miljković, Biljana, Spalević, Ljiljana, Vojinović, Dragica, Pavlović, Ivan, Mićić, Mileva, Živković, Radmila, "Review of new multicausal chronical poultry disease" in Savremena poljoprivreda, 51, no. 3-4 (2002):301-308,
https://hdl.handle.net/21.15107/rcub_intor_144 .