TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Montanaro, Jacqueline
AU  - Stein, Elisabeth
AU  - Bintner, Nora
AU  - Schuerer, Nadine
AU  - Ladurner, Angela
AU  - Barisani-Asenbauer, Talin
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/404
PB  - Wiley-Blackwell, Hoboken
C3  - Immunology
T1  - Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis
EP  - 25
SP  - 25
VL  - 143
UR  - https://hdl.handle.net/21.15107/rcub_intor_404
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Montanaro, Jacqueline and Stein, Elisabeth and Bintner, Nora and Schuerer, Nadine and Ladurner, Angela and Barisani-Asenbauer, Talin",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Immunology",
title = "Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis",
pages = "25-25",
volume = "143",
url = "https://hdl.handle.net/21.15107/rcub_intor_404"
}

Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Montanaro, J., Stein, E., Bintner, N., Schuerer, N., Ladurner, A.,& Barisani-Asenbauer, T.. (2014). Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis. in Immunology
Wiley-Blackwell, Hoboken., 143, 25-25.
https://hdl.handle.net/21.15107/rcub_intor_404

Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Montanaro J, Stein E, Bintner N, Schuerer N, Ladurner A, Barisani-Asenbauer T. Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis. in Immunology. 2014;143:25-25.
https://hdl.handle.net/21.15107/rcub_intor_404 .

Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Montanaro, Jacqueline, Stein, Elisabeth, Bintner, Nora, Schuerer, Nadine, Ladurner, Angela, Barisani-Asenbauer, Talin, "Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis" in Immunology, 143 (2014):25-25,
https://hdl.handle.net/21.15107/rcub_intor_404 .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Stein, Elisabeth
AU  - Ladurner, Angela
AU  - Mayr, Ulrike Beate
AU  - Lubitz, Werner
AU  - Barisani-Asenbauer, Talin
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/415
AB  - Purpose: In this work we explored the efficacies of Salmonella typhimurium (ST) Bacterial Ghosts (BGs), using the conjunctival route of immunization, to induce mucosal and systemic immunity against ST.

Methods: BALB/c and C57BL/6 mice were immunized via the conjunctiva with ST BGs in three different doses (50, 25 and 12.5 μg/mouse). Three immunizations were performed at 2 weeks interval and the evaluation of local and systemic immune response was done 2 and 8 weeks after the last immunization. This research was conducted in compliance with the “ARVO Statement of the Use of Animals in Ophthalmic and Visual Research” and the “Declaration of Helsinki”.

Results: ST BGs were well tolerated; non-toxic and successfully uptaken by the mouse conjunctival cells. We have found ST BG-specific IgG and IgA in tears and sera of both mice strains, as well as, IgG positive ST BG-specific cells. It was shown that the amount of ST BG-specific antibodies in mice' sera strongly correlated with the presence of ST BG-specific B cells in draining lymph nodes. 8 weeks after the last immunization, the levels of anti-ST BG IgG and IgA in the sera of BALB/c mice were higher than in C57BL/6. BALB/c mice exhibited also more prominent anti-ST BG IgA concentration in mice’ tears. The strongest IgG and IgA response was obtained with the highest dose used for immunization (50 μg/mouse). The conjunctival application of ST BGs in both mouse strains induced skewing of ST BG-specific immune responses toward a Th1/Th17 profile, as determined by the stimulation of IFNγ and IL-17A secretion and/or the concurrent pronounced reduction of IL-4 secretion.

Conclusions: Conjunctival immunization induced a strong ST BG-specific local and systemic immune response, and a Th1-biased response that is critical for control of Salmonella species. ST BGs, non-living shells of Gram negative ST bacteria, possess excellent safety properties and could be used as a candidate vaccine for an ocular delivery.
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts
IS  - 13
VL  - 55
UR  - https://hdl.handle.net/21.15107/rcub_intor_415
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Stein, Elisabeth and Ladurner, Angela and Mayr, Ulrike Beate and Lubitz, Werner and Barisani-Asenbauer, Talin",
year = "2014",
abstract = "Purpose: In this work we explored the efficacies of Salmonella typhimurium (ST) Bacterial Ghosts (BGs), using the conjunctival route of immunization, to induce mucosal and systemic immunity against ST.

Methods: BALB/c and C57BL/6 mice were immunized via the conjunctiva with ST BGs in three different doses (50, 25 and 12.5 μg/mouse). Three immunizations were performed at 2 weeks interval and the evaluation of local and systemic immune response was done 2 and 8 weeks after the last immunization. This research was conducted in compliance with the “ARVO Statement of the Use of Animals in Ophthalmic and Visual Research” and the “Declaration of Helsinki”.

Results: ST BGs were well tolerated; non-toxic and successfully uptaken by the mouse conjunctival cells. We have found ST BG-specific IgG and IgA in tears and sera of both mice strains, as well as, IgG positive ST BG-specific cells. It was shown that the amount of ST BG-specific antibodies in mice' sera strongly correlated with the presence of ST BG-specific B cells in draining lymph nodes. 8 weeks after the last immunization, the levels of anti-ST BG IgG and IgA in the sera of BALB/c mice were higher than in C57BL/6. BALB/c mice exhibited also more prominent anti-ST BG IgA concentration in mice’ tears. The strongest IgG and IgA response was obtained with the highest dose used for immunization (50 μg/mouse). The conjunctival application of ST BGs in both mouse strains induced skewing of ST BG-specific immune responses toward a Th1/Th17 profile, as determined by the stimulation of IFNγ and IL-17A secretion and/or the concurrent pronounced reduction of IL-4 secretion.

Conclusions: Conjunctival immunization induced a strong ST BG-specific local and systemic immune response, and a Th1-biased response that is critical for control of Salmonella species. ST BGs, non-living shells of Gram negative ST bacteria, possess excellent safety properties and could be used as a candidate vaccine for an ocular delivery.",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts",
number = "13",
volume = "55",
url = "https://hdl.handle.net/21.15107/rcub_intor_415"
}

Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Stein, E., Ladurner, A., Mayr, U. B., Lubitz, W.,& Barisani-Asenbauer, T.. (2014). The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 55(13).
https://hdl.handle.net/21.15107/rcub_intor_415

Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Stein E, Ladurner A, Mayr UB, Lubitz W, Barisani-Asenbauer T. The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts. in Investigative Ophthalmology & Visual Science. 2014;55(13).
https://hdl.handle.net/21.15107/rcub_intor_415 .

Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Stein, Elisabeth, Ladurner, Angela, Mayr, Ulrike Beate, Lubitz, Werner, Barisani-Asenbauer, Talin, "The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts" in Investigative Ophthalmology & Visual Science, 55, no. 13 (2014),
https://hdl.handle.net/21.15107/rcub_intor_415 .