TY  - JOUR
AU  - Jovčić, Branko
AU  - Bertani, Iris
AU  - Venturi, Vittorio
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/333
UR  - http://intor.torlakinstitut.com/handle/123456789/679
AB  - The sigma(S) subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.
PB  - Microbiology Soc Korea, Seoul
T2  - Journal of Microbiology
T1  - 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation
EP  - 61
IS  - 1
SP  - 56
VL  - 46
DO  - 10.1007/s12275-007-0127-2
ER  - 

@article{
author = "Jovčić, Branko and Bertani, Iris and Venturi, Vittorio and Topisirović, Ljubiša and Kojić, Milan",
year = "2008",
abstract = "The sigma(S) subunit of RNA polymerase is a central regulator which governs the expression of a host of stationary phase-induced and osmotically regulated genes in Gram-negative bacteria. The Pseudomonas putida rpoS gene is transcribed as a monocistronic rpoS mRNA with a 368 nucleotide-long 5' untranslated region (5' UTR). In this study, we investigate the posttranscriptional control of RpoS synthesis using rpoS-lacZ transcriptional and translational fusions consisting of the native promoter and deletions of 5' UTR or insertion into UTR. The differing activity of constructed translational fusions strongly indicated that the 5' UTR is involved in the translational regulation of RpoS expression in the stationary phase. The results obtained herein demonstrated that the structure of UTR performs an important function in the translational regulation of the rpoS gene.",
publisher = "Microbiology Soc Korea, Seoul",
journal = "Journal of Microbiology",
title = "5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation",
pages = "61-56",
number = "1",
volume = "46",
doi = "10.1007/s12275-007-0127-2"
}

Jovčić, B., Bertani, I., Venturi, V., Topisirović, L.,& Kojić, M.. (2008). 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. in Journal of Microbiology
Microbiology Soc Korea, Seoul., 46(1), 56-61.
https://doi.org/10.1007/s12275-007-0127-2

Jovčić B, Bertani I, Venturi V, Topisirović L, Kojić M. 5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation. in Journal of Microbiology. 2008;46(1):56-61.
doi:10.1007/s12275-007-0127-2 .

Jovčić, Branko, Bertani, Iris, Venturi, Vittorio, Topisirović, Ljubiša, Kojić, Milan, "5 ' untranslated region of the Pseudomonas putida WCS358 stationary phase sigma factor rpoS mRNA is involved in RpoS translational regulation" in Journal of Microbiology, 46, no. 1 (2008):56-61,
https://doi.org/10.1007/s12275-007-0127-2 . .