TY  - JOUR
AU  - Maslovarić, Irina
AU  - Vukov, Nataša
AU  - Stojković, Aleksandra
AU  - Kosanović, Dejana
AU  - Jovanova-Nešić, Katica
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/368
AB  - Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund's adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mice.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic
EP  - 1322
IS  - 4
SP  - 1317
VL  - 65
DO  - 10.2298/ABS1304317M
ER  - 

@article{
author = "Maslovarić, Irina and Vukov, Nataša and Stojković, Aleksandra and Kosanović, Dejana and Jovanova-Nešić, Katica",
year = "2013",
abstract = "Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund's adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mice.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic",
pages = "1322-1317",
number = "4",
volume = "65",
doi = "10.2298/ABS1304317M"
}

Maslovarić, I., Vukov, N., Stojković, A., Kosanović, D.,& Jovanova-Nešić, K.. (2013). Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(4), 1317-1322.
https://doi.org/10.2298/ABS1304317M

Maslovarić I, Vukov N, Stojković A, Kosanović D, Jovanova-Nešić K. Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic. in Archives of Biological Sciences. 2013;65(4):1317-1322.
doi:10.2298/ABS1304317M .

Maslovarić, Irina, Vukov, Nataša, Stojković, Aleksandra, Kosanović, Dejana, Jovanova-Nešić, Katica, "Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic" in Archives of Biological Sciences, 65, no. 4 (2013):1317-1322,
https://doi.org/10.2298/ABS1304317M . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Vojnović, Sandra
AU  - Vukov, Nataša
AU  - Vasiljević, Branka
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/813
AB  - 16S rRNK metilaze su eksprimirane u većini bakterija koje proizvode antibiotike da bi se zaštitile od dejstva antibiotika putem metilacije 16S rRNK na pozicijama koje su bitne za njihovo dejstvo. Gen sgm koji je odgovoran za rezistenciju na sisomicin i gentamicin u soju Micromonospora zionensis, metilu je G1405 u okviru A mesta 16S rRNA gde se nalazi i CCGCCC heksanukleotid. Isti heksanukleotid se nalazi i 14 nukleotida ispred mesta vezivanja ribozoma na sgm informacionoj RNK. Predloženi model translacione regulacije sgm gena pretpostavlja da se Sgm protein vezuje za ovaj motiv kako na 16S rRNK, tako i na 5’ netranslirajućem regionu (UTR) sopstvene informacione RNK. 5’ UTR sekvenca je overeksprimirana na sgm informacionoj RNK sa skraćenim 3’ krajem i testiran je efekat na gentamicinsku rezistenciju u ćelijama E. coli i Micromonospora melanosporea. Overekspresija ove regulatorne sekvence dovodi do smanjenja rezistencije u oba testirana soja najverovatnije zbog titracije Sgm molekula od strane 5’ UTR-a.
AB  - The 16S rRNA methylases are expressed by most of the antibiotic producing bacteria in order to protect themselves against antibiotics by methylation of 16S rRNA at positions which are crucial for their action. The sgm sisomicin-gentamicin resistance gene from Micromonospora zionensis methylates G1405 positioned in the A site of 16S rRNA, which includes a CCGCCC hexanucleotide. The same hexanucleotide is also present 14 nucleotides in front of the ribosome binding site of sgm mRNA. The model proposed for translational regulation of sgm assumes that Sgm binds to this motif, both on 16S rRNA and on the 5’ untranslated region (UTR) of its own mRNA. The 5’ UTR mRNA sequence was overexpressed on 3’-truncated sgm mRNA, and the effect on gentamicin resistance conferred by Sgm was tested in Escherichia coli and in Micromonospora melanosporea. Overexpression of the sgm mRNA regulatory region decreases the resistance to gentamicin in both E. coli and M. melanosporea. This effect is likely to be due to titration of Sgm molecules by the overexpressed 5’ UTR.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Overekspresija 5’ UTR mRNK sgm gena smanjuje rezistenciju na gentamicin u ćelijama Escherichia coli i Micromonospora melanosporea
T1  - Overexpression of sgm 5’ UTR mRNA reduces gentamicin resistance in both Escherichia coli and Micromonospora melanosporea cells
EP  - 280
IS  - 4
SP  - 273
VL  - 59
DO  - 10.2298/ABS0704273K
ER  - 

@article{
author = "Kojić, Milan and Vojnović, Sandra and Vukov, Nataša and Vasiljević, Branka",
year = "2007",
abstract = "16S rRNK metilaze su eksprimirane u većini bakterija koje proizvode antibiotike da bi se zaštitile od dejstva antibiotika putem metilacije 16S rRNK na pozicijama koje su bitne za njihovo dejstvo. Gen sgm koji je odgovoran za rezistenciju na sisomicin i gentamicin u soju Micromonospora zionensis, metilu je G1405 u okviru A mesta 16S rRNA gde se nalazi i CCGCCC heksanukleotid. Isti heksanukleotid se nalazi i 14 nukleotida ispred mesta vezivanja ribozoma na sgm informacionoj RNK. Predloženi model translacione regulacije sgm gena pretpostavlja da se Sgm protein vezuje za ovaj motiv kako na 16S rRNK, tako i na 5’ netranslirajućem regionu (UTR) sopstvene informacione RNK. 5’ UTR sekvenca je overeksprimirana na sgm informacionoj RNK sa skraćenim 3’ krajem i testiran je efekat na gentamicinsku rezistenciju u ćelijama E. coli i Micromonospora melanosporea. Overekspresija ove regulatorne sekvence dovodi do smanjenja rezistencije u oba testirana soja najverovatnije zbog titracije Sgm molekula od strane 5’ UTR-a., The 16S rRNA methylases are expressed by most of the antibiotic producing bacteria in order to protect themselves against antibiotics by methylation of 16S rRNA at positions which are crucial for their action. The sgm sisomicin-gentamicin resistance gene from Micromonospora zionensis methylates G1405 positioned in the A site of 16S rRNA, which includes a CCGCCC hexanucleotide. The same hexanucleotide is also present 14 nucleotides in front of the ribosome binding site of sgm mRNA. The model proposed for translational regulation of sgm assumes that Sgm binds to this motif, both on 16S rRNA and on the 5’ untranslated region (UTR) of its own mRNA. The 5’ UTR mRNA sequence was overexpressed on 3’-truncated sgm mRNA, and the effect on gentamicin resistance conferred by Sgm was tested in Escherichia coli and in Micromonospora melanosporea. Overexpression of the sgm mRNA regulatory region decreases the resistance to gentamicin in both E. coli and M. melanosporea. This effect is likely to be due to titration of Sgm molecules by the overexpressed 5’ UTR.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Overekspresija 5’ UTR mRNK sgm gena smanjuje rezistenciju na gentamicin u ćelijama Escherichia coli i Micromonospora melanosporea, Overexpression of sgm 5’ UTR mRNA reduces gentamicin resistance in both Escherichia coli and Micromonospora melanosporea cells",
pages = "280-273",
number = "4",
volume = "59",
doi = "10.2298/ABS0704273K"
}

Kojić, M., Vojnović, S., Vukov, N.,& Vasiljević, B.. (2007). Overekspresija 5’ UTR mRNK sgm gena smanjuje rezistenciju na gentamicin u ćelijama Escherichia coli i Micromonospora melanosporea. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 59(4), 273-280.
https://doi.org/10.2298/ABS0704273K

Kojić M, Vojnović S, Vukov N, Vasiljević B. Overekspresija 5’ UTR mRNK sgm gena smanjuje rezistenciju na gentamicin u ćelijama Escherichia coli i Micromonospora melanosporea. in Archives of Biological Sciences. 2007;59(4):273-280.
doi:10.2298/ABS0704273K .

Kojić, Milan, Vojnović, Sandra, Vukov, Nataša, Vasiljević, Branka, "Overekspresija 5’ UTR mRNK sgm gena smanjuje rezistenciju na gentamicin u ćelijama Escherichia coli i Micromonospora melanosporea" in Archives of Biological Sciences, 59, no. 4 (2007):273-280,
https://doi.org/10.2298/ABS0704273K . .