TY  - JOUR
AU  - Kosanović, Dejana
AU  - Sheehan, Gerard
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/559
AB  - Green mould disease is caused by Trichoderma aggressivum which colonizes mushroom compost and reduces yield. Two Pseudomonas species are associated with mushroom compost: Pseudomonas putida, which stimulates mushroom pinning, and Pseudomonas tolaasii which has a negative effect on crop production. The aim of this work was to characterize T. aggressivum – Pseudomonas interactions as these may be important factors in the development of green mould disease. P. tolaasii supernatant inhibited growth by 57% but P. putida stimulated growth of T.aggressivum by 44%. Tolaasin production was identified in P. tolaasii cultures with a peak at 96 h. Fluorescent microscopy examination of T. aggressivum hyphae revealed that exposure to P. tolaasii supernatant decreased mycelial formation while increasing the abundance of conidia. Label free proteomic analysis of changes in the abundance of T. aggressivum proteins indicated that exposure to P. tolaasii supernatant lead to an oxidative stress response and catabolic enzyme activation (mitochondrial import inner membrane translocase complex (5.7-fold), oxidoreductase (5.2-fold), glucoamylase (5.1-fold)). Exposure of T. aggressivum to P. putida supernatant lead to an increase in the abundance of proteins associated with growth and development (structural constituents of ribosome (20-fold), H/ACA ribonucleoprotein complex subunit (18-fold), DNA binding and nucleosome assembly protein (5.3-fold), and prefoldin (5-fold)). These results indicate that exposure to P. putida can stimulate the growth of T. aggressivum and this interaction may be an important factor in increasing green mould disease in mushroom crops and so reducing yield.
T2  - European Journal of Plant Pathology
T1  - Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum
EP  - 121
IS  - 1
SP  - 111
VL  - 156
DO  - 10.1007/s10658-019-01867-z
UR  - https://hdl.handle.net/21.15107/rcub_intor_559
ER  - 

@article{
author = "Kosanović, Dejana and Sheehan, Gerard and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Green mould disease is caused by Trichoderma aggressivum which colonizes mushroom compost and reduces yield. Two Pseudomonas species are associated with mushroom compost: Pseudomonas putida, which stimulates mushroom pinning, and Pseudomonas tolaasii which has a negative effect on crop production. The aim of this work was to characterize T. aggressivum – Pseudomonas interactions as these may be important factors in the development of green mould disease. P. tolaasii supernatant inhibited growth by 57% but P. putida stimulated growth of T.aggressivum by 44%. Tolaasin production was identified in P. tolaasii cultures with a peak at 96 h. Fluorescent microscopy examination of T. aggressivum hyphae revealed that exposure to P. tolaasii supernatant decreased mycelial formation while increasing the abundance of conidia. Label free proteomic analysis of changes in the abundance of T. aggressivum proteins indicated that exposure to P. tolaasii supernatant lead to an oxidative stress response and catabolic enzyme activation (mitochondrial import inner membrane translocase complex (5.7-fold), oxidoreductase (5.2-fold), glucoamylase (5.1-fold)). Exposure of T. aggressivum to P. putida supernatant lead to an increase in the abundance of proteins associated with growth and development (structural constituents of ribosome (20-fold), H/ACA ribonucleoprotein complex subunit (18-fold), DNA binding and nucleosome assembly protein (5.3-fold), and prefoldin (5-fold)). These results indicate that exposure to P. putida can stimulate the growth of T. aggressivum and this interaction may be an important factor in increasing green mould disease in mushroom crops and so reducing yield.",
journal = "European Journal of Plant Pathology",
title = "Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum",
pages = "121-111",
number = "1",
volume = "156",
doi = "10.1007/s10658-019-01867-z",
url = "https://hdl.handle.net/21.15107/rcub_intor_559"
}

Kosanović, D., Sheehan, G., Grogan, H.,& Kavanagh, K.. (2020). Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum. in European Journal of Plant Pathology, 156(1), 111-121.
https://doi.org/10.1007/s10658-019-01867-z
https://hdl.handle.net/21.15107/rcub_intor_559

Kosanović D, Sheehan G, Grogan H, Kavanagh K. Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum. in European Journal of Plant Pathology. 2020;156(1):111-121.
doi:10.1007/s10658-019-01867-z
https://hdl.handle.net/21.15107/rcub_intor_559 .

Kosanović, Dejana, Sheehan, Gerard, Grogan, Helen, Kavanagh, Kevin, "Characterisation of the interaction of Pseudomonas putida and Pseudomonas tolaasii with Trichoderma aggressivum" in European Journal of Plant Pathology, 156, no. 1 (2020):111-121,
https://doi.org/10.1007/s10658-019-01867-z .,
https://hdl.handle.net/21.15107/rcub_intor_559 .

TY  - DATA
AU  - Kosanović, Dejana
AU  - Sheehan, Gerard
AU  - Grogan, Helen
AU  - Kavanagh, Kevin
PY  - 2020
UR  - http://intor.torlakinstitut.com/handle/123456789/641
AB  - Figure S1. Pathogenicity assay on mushroom caps. Top of the pilei were inoculated with 20 μl of Pseudomonas tolaasii (1×1013 CFU ml−1). Sterile water was used as a negative control. Brown blotch on mushroom caps, 48h after inoculation (b), no blotch on a control (a); Figure S2. Zones of Inhibition due to P. putida (a, b) and P. tolaasii (c, d) supernatants on PDA (b, d) and ME (a, c) plates inoculated with with 104 T. aggressivum conidia and wells filled with 50 µl of 96h SN, after 48h, 30 °C; Figure S3. Comparison of superimposed P. putida and P. tolaasii supernatant chromatographs. Tolaasin Rt = 17.418 min. detected only in 96- and 120-hours P. tolaasii supernatants; Figure S4. PCA analysis. ◦ - P. putida group, ∆ - P. tolaasii group and ▫- Control group; Figure S5. Gene Ontology analysis by Blast2GO software tool. Biological process, molecular function, and cellular components significantly enriched within the proteome of P. putida/tolaasii treated T. aggressivum.
T2  - European Journal of Plant Pathology
T1  - Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.
EP  - 121
IS  - 1
SP  - 111
VL  - 156
UR  - https://hdl.handle.net/21.15107/rcub_intor_641
ER  - 

@misc{
author = "Kosanović, Dejana and Sheehan, Gerard and Grogan, Helen and Kavanagh, Kevin",
year = "2020",
abstract = "Figure S1. Pathogenicity assay on mushroom caps. Top of the pilei were inoculated with 20 μl of Pseudomonas tolaasii (1×1013 CFU ml−1). Sterile water was used as a negative control. Brown blotch on mushroom caps, 48h after inoculation (b), no blotch on a control (a); Figure S2. Zones of Inhibition due to P. putida (a, b) and P. tolaasii (c, d) supernatants on PDA (b, d) and ME (a, c) plates inoculated with with 104 T. aggressivum conidia and wells filled with 50 µl of 96h SN, after 48h, 30 °C; Figure S3. Comparison of superimposed P. putida and P. tolaasii supernatant chromatographs. Tolaasin Rt = 17.418 min. detected only in 96- and 120-hours P. tolaasii supernatants; Figure S4. PCA analysis. ◦ - P. putida group, ∆ - P. tolaasii group and ▫- Control group; Figure S5. Gene Ontology analysis by Blast2GO software tool. Biological process, molecular function, and cellular components significantly enriched within the proteome of P. putida/tolaasii treated T. aggressivum.",
journal = "European Journal of Plant Pathology",
title = "Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.",
pages = "121-111",
number = "1",
volume = "156",
url = "https://hdl.handle.net/21.15107/rcub_intor_641"
}

Kosanović, D., Sheehan, G., Grogan, H.,& Kavanagh, K.. (2020). Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.. in European Journal of Plant Pathology, 156(1), 111-121.
https://hdl.handle.net/21.15107/rcub_intor_641

Kosanović D, Sheehan G, Grogan H, Kavanagh K. Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z.. in European Journal of Plant Pathology. 2020;156(1):111-121.
https://hdl.handle.net/21.15107/rcub_intor_641 .

Kosanović, Dejana, Sheehan, Gerard, Grogan, Helen, Kavanagh, Kevin, "Supplementary information for the article: Kosanović, D.; Sheehan, G.; Grogan, H.; Kavanagh, K. Characterisation of the Interaction of Pseudomonas Putida and Pseudomonas Tolaasii with Trichoderma Aggressivum. European Journal of Plant Pathology 2020, 156 (1), 111–121. https://doi.org/10.1007/s10658-019-01867-z." in European Journal of Plant Pathology, 156, no. 1 (2020):111-121,
https://hdl.handle.net/21.15107/rcub_intor_641 .