Eijsink, Vincent G.H.

Link to this page

Authority KeyName Variants
bca8b8b0-3d6d-41ea-b1ce-e8e20f3c93b0
  • Eijsink, Vincent G.H. (2)
Projects

Author's Bibliography

Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens

Kuczkowska, Katarzyna; Kleiveland, Charlotte R.; Minić, Rajna; Moen, Lars F.; Overland, Lise; Tjaland, Rannei; Carlsen, Harald; Lea, Tor; Mathiesen, Geir; Eijsink, Vincent G.H.

(Amer Soc Microbiology, Washington, 2017)

TY  - JOUR
AU  - Kuczkowska, Katarzyna
AU  - Kleiveland, Charlotte R.
AU  - Minić, Rajna
AU  - Moen, Lars F.
AU  - Overland, Lise
AU  - Tjaland, Rannei
AU  - Carlsen, Harald
AU  - Lea, Tor
AU  - Mathiesen, Geir
AU  - Eijsink, Vincent G.H.
PY  - 2017
UR  - http://intor.torlakinstitut.com/handle/123456789/492
AB  - Tuberculosis (TB) remains among the most deadly diseases in the world. The only available vaccine against tuberculosis is the bacille Calmette-Guerin (BCG) vaccine, which does not ensure full protection in adults. There is a global urgency for the development of an effective vaccine for preventing disease transmission, and it requires novel approaches. We are exploring the use of lactic acid bacteria (LAB) as a vector for antigen delivery to mucosal sites. Here, we demonstrate the successful expression and surface display of a Mycobacterium tuberculosis fusion antigen (comprising Ag85B and ESAT-6, referred to as AgE6) on Lactobacillus plantarum. The AgE6 fusion antigen was targeted to the bacterial surface using two different anchors, a lipoprotein anchor directing the protein to the cell membrane and a covalent cell wall anchor. AgE6-producing L. plantarum strains using each of the two anchors induced antigen-specific proliferative responses in lymphocytes purified from TB-positive donors. Similarly, both strains induced immune responses in mice after nasal or oral immunization. The impact of the anchoring strategies was reflected in dissimilarities in the immune responses generated by the two L. plantarum strains in vivo. The present study comprises an initial step toward the development of L. plantarum as a vector for M. tuberculosis antigen delivery. IMPORTANCE This work presents the development of Lactobacillus plantarum as a candidate mucosal vaccine against tuberculosis. Tuberculosis remains one of the top infectious diseases worldwide, and the only available vaccine, bacille Calmette-Guerin (BCG), fails to protect adults and adolescents. Direct antigen delivery to mucosal sites is a promising strategy in tuberculosis vaccine development, and lactic acid bacteria potentially provide easy, safe, and low-cost delivery vehicles for mucosal immunization. We have engineered L. plantarum strains to produce a Mycobacterium tuberculosis fusion antigen and to anchor this antigen to the bacterial cell wall or to the cell membrane. The recombinant strains elicited proliferative antigenspecific T-cell responses in white blood cells from tuberculosis-positive humans and induced specific immune responses after nasal and oral administrations in mice.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens
IS  - 2
VL  - 83
DO  - 10.1128/AEM.02782-16
UR  - conv_403
ER  - 
@article{
author = "Kuczkowska, Katarzyna and Kleiveland, Charlotte R. and Minić, Rajna and Moen, Lars F. and Overland, Lise and Tjaland, Rannei and Carlsen, Harald and Lea, Tor and Mathiesen, Geir and Eijsink, Vincent G.H.",
year = "2017",
abstract = "Tuberculosis (TB) remains among the most deadly diseases in the world. The only available vaccine against tuberculosis is the bacille Calmette-Guerin (BCG) vaccine, which does not ensure full protection in adults. There is a global urgency for the development of an effective vaccine for preventing disease transmission, and it requires novel approaches. We are exploring the use of lactic acid bacteria (LAB) as a vector for antigen delivery to mucosal sites. Here, we demonstrate the successful expression and surface display of a Mycobacterium tuberculosis fusion antigen (comprising Ag85B and ESAT-6, referred to as AgE6) on Lactobacillus plantarum. The AgE6 fusion antigen was targeted to the bacterial surface using two different anchors, a lipoprotein anchor directing the protein to the cell membrane and a covalent cell wall anchor. AgE6-producing L. plantarum strains using each of the two anchors induced antigen-specific proliferative responses in lymphocytes purified from TB-positive donors. Similarly, both strains induced immune responses in mice after nasal or oral immunization. The impact of the anchoring strategies was reflected in dissimilarities in the immune responses generated by the two L. plantarum strains in vivo. The present study comprises an initial step toward the development of L. plantarum as a vector for M. tuberculosis antigen delivery. IMPORTANCE This work presents the development of Lactobacillus plantarum as a candidate mucosal vaccine against tuberculosis. Tuberculosis remains one of the top infectious diseases worldwide, and the only available vaccine, bacille Calmette-Guerin (BCG), fails to protect adults and adolescents. Direct antigen delivery to mucosal sites is a promising strategy in tuberculosis vaccine development, and lactic acid bacteria potentially provide easy, safe, and low-cost delivery vehicles for mucosal immunization. We have engineered L. plantarum strains to produce a Mycobacterium tuberculosis fusion antigen and to anchor this antigen to the bacterial cell wall or to the cell membrane. The recombinant strains elicited proliferative antigenspecific T-cell responses in white blood cells from tuberculosis-positive humans and induced specific immune responses after nasal and oral administrations in mice.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens",
number = "2",
volume = "83",
doi = "10.1128/AEM.02782-16",
url = "conv_403"
}
Kuczkowska, K., Kleiveland, C. R., Minić, R., Moen, L. F., Overland, L., Tjaland, R., Carlsen, H., Lea, T., Mathiesen, G.,& Eijsink, V. G.H.. (2017). Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 83(2).
https://doi.org/10.1128/AEM.02782-16
conv_403
Kuczkowska K, Kleiveland CR, Minić R, Moen LF, Overland L, Tjaland R, Carlsen H, Lea T, Mathiesen G, Eijsink VG. Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens. in Applied and Environmental Microbiology. 2017;83(2).
doi:10.1128/AEM.02782-16
conv_403 .
Kuczkowska, Katarzyna, Kleiveland, Charlotte R., Minić, Rajna, Moen, Lars F., Overland, Lise, Tjaland, Rannei, Carlsen, Harald, Lea, Tor, Mathiesen, Geir, Eijsink, Vincent G.H., "Immunogenic Properties of Lactobacillus plantarum Producing Surface-Displayed Mycobacterium tuberculosis Antigens" in Applied and Environmental Microbiology, 83, no. 2 (2017),
https://doi.org/10.1128/AEM.02782-16 .,
conv_403 .
3
31
24
28

Effects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in mice

Minić, Rajna; Gavrović-Jankulović, Marija; Petrušić, Vladimir; Živković, Irena; Eijsink, Vincent G.H.; Dimitrijević, Ljiljana; Mathiesen, Geir

(Elsevier Science Bv, Amsterdam, 2015)

TY  - JOUR
AU  - Minić, Rajna
AU  - Gavrović-Jankulović, Marija
AU  - Petrušić, Vladimir
AU  - Živković, Irena
AU  - Eijsink, Vincent G.H.
AU  - Dimitrijević, Ljiljana
AU  - Mathiesen, Geir
PY  - 2015
UR  - http://intor.torlakinstitut.com/handle/123456789/447
AB  - Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinant Fes p1 allergen, from Festuca pratensis was tested in a mouse model of Fes p1 allergy. For surface expression of Fes p1 allergen in L. plantarum WCFS1 pSIP system with inducible expression was used. Balb/c mice were sensitized with Fes p1 protein in alum and subsequently received live recombinant L. plantarum orally. Antibody levels (IgE, total IgG, IgG1, IgG2a, and IgA) were determined by ELISA. Differential eosinophil count in peripheral blood was performed. Reduced peripheral blood eosinophilia and increased serum IgG2A levels was detected in both groups which received live L. plantarum orally. Specific serum IgA levels were increased only in mice treated with the recombinant bacteria. Oral application of L. plantarum WCFS1 has a beneficial therapeutic effect in a mouse model of Fes p1 allergy. Cell surface expression of Fes p1 allergen potentiates this phenomenon in an allergen specific way. (C) 2015 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - Effects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in mice
EP  - 28
SP  - 23
VL  - 199
DO  - 10.1016/j.jbiotec.2015.01.028
UR  - conv_357
ER  - 
@article{
author = "Minić, Rajna and Gavrović-Jankulović, Marija and Petrušić, Vladimir and Živković, Irena and Eijsink, Vincent G.H. and Dimitrijević, Ljiljana and Mathiesen, Geir",
year = "2015",
abstract = "Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinant Fes p1 allergen, from Festuca pratensis was tested in a mouse model of Fes p1 allergy. For surface expression of Fes p1 allergen in L. plantarum WCFS1 pSIP system with inducible expression was used. Balb/c mice were sensitized with Fes p1 protein in alum and subsequently received live recombinant L. plantarum orally. Antibody levels (IgE, total IgG, IgG1, IgG2a, and IgA) were determined by ELISA. Differential eosinophil count in peripheral blood was performed. Reduced peripheral blood eosinophilia and increased serum IgG2A levels was detected in both groups which received live L. plantarum orally. Specific serum IgA levels were increased only in mice treated with the recombinant bacteria. Oral application of L. plantarum WCFS1 has a beneficial therapeutic effect in a mouse model of Fes p1 allergy. Cell surface expression of Fes p1 allergen potentiates this phenomenon in an allergen specific way. (C) 2015 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Effects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in mice",
pages = "28-23",
volume = "199",
doi = "10.1016/j.jbiotec.2015.01.028",
url = "conv_357"
}
Minić, R., Gavrović-Jankulović, M., Petrušić, V., Živković, I., Eijsink, V. G.H., Dimitrijević, L.,& Mathiesen, G.. (2015). Effects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in mice. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 199, 23-28.
https://doi.org/10.1016/j.jbiotec.2015.01.028
conv_357
Minić R, Gavrović-Jankulović M, Petrušić V, Živković I, Eijsink VG, Dimitrijević L, Mathiesen G. Effects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in mice. in Journal of Biotechnology. 2015;199:23-28.
doi:10.1016/j.jbiotec.2015.01.028
conv_357 .
Minić, Rajna, Gavrović-Jankulović, Marija, Petrušić, Vladimir, Živković, Irena, Eijsink, Vincent G.H., Dimitrijević, Ljiljana, Mathiesen, Geir, "Effects of orally applied Fes p1-displaying L. plantarum WCFS1 on Fes p1 induced allergy in mice" in Journal of Biotechnology, 199 (2015):23-28,
https://doi.org/10.1016/j.jbiotec.2015.01.028 .,
conv_357 .
1
14
11
12