TY  - JOUR
AU  - Dimitrijević, Ljiljana
AU  - Stojanović, M.
AU  - Cirić, B.
AU  - Radulović, M.
AU  - Stojanović, R.
AU  - Popović, Zoran
AU  - Inić-Kanada, Aleksandra
AU  - Živković, Irena
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/178
AB  - In this paper we report data regarding the IgM Y7 cross-reactive idiotope (CRIo) obtained by analysis of: 1) its V-gene subgroup dependance, 2) the frequency of its expression on human monoclonal IgMs and IgM molecules from normal and pathological sera. Furthermore, comparison of epitopic repertoire and nature of binding of human monoclonal IgMs expressing Y7 CRIo was performed to confirm the natural antibody properties of these molecules. IgM isolated from sera of patient DJ (IgM DJ) which expresses the Y7 idiotope has been classified to V(H)3/V(L)2 subgroup. From ten IgMs tested only IgM from patient RD (IgM RD) has been shown to express Y7 idiotope. Y7(+) human IgMs bound to ssDNA, lactic acid bacteria, mouse laminin, porcine thyroglobulin and mouse IgG. Higher percentage of the expression of Y7 CRIo was detected in the sera of patients suffering from autoimmune diseases such as lupus, rheumatoid arthritis and psoriasis vulgaris as well as in patients suffering from chronic infections of the lower urinary tract. Antigen binding repertoire and properties of Y7(+) monoclonal IgM, frequency of Y7 expression on monoclonal IgMs and its concentration in normal and pathological sera indicate the important biological role of this CRIo within the immune system.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Immunological Investigations
T1  - Expression of Y7 cross-reactive idiotope on human IgM molecules
EP  - 14
IS  - 1
SP  - 1
VL  - 33
DO  - 10.1081/IMM-120027680
ER  - 

@article{
author = "Dimitrijević, Ljiljana and Stojanović, M. and Cirić, B. and Radulović, M. and Stojanović, R. and Popović, Zoran and Inić-Kanada, Aleksandra and Živković, Irena",
year = "2004",
abstract = "In this paper we report data regarding the IgM Y7 cross-reactive idiotope (CRIo) obtained by analysis of: 1) its V-gene subgroup dependance, 2) the frequency of its expression on human monoclonal IgMs and IgM molecules from normal and pathological sera. Furthermore, comparison of epitopic repertoire and nature of binding of human monoclonal IgMs expressing Y7 CRIo was performed to confirm the natural antibody properties of these molecules. IgM isolated from sera of patient DJ (IgM DJ) which expresses the Y7 idiotope has been classified to V(H)3/V(L)2 subgroup. From ten IgMs tested only IgM from patient RD (IgM RD) has been shown to express Y7 idiotope. Y7(+) human IgMs bound to ssDNA, lactic acid bacteria, mouse laminin, porcine thyroglobulin and mouse IgG. Higher percentage of the expression of Y7 CRIo was detected in the sera of patients suffering from autoimmune diseases such as lupus, rheumatoid arthritis and psoriasis vulgaris as well as in patients suffering from chronic infections of the lower urinary tract. Antigen binding repertoire and properties of Y7(+) monoclonal IgM, frequency of Y7 expression on monoclonal IgMs and its concentration in normal and pathological sera indicate the important biological role of this CRIo within the immune system.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Immunological Investigations",
title = "Expression of Y7 cross-reactive idiotope on human IgM molecules",
pages = "14-1",
number = "1",
volume = "33",
doi = "10.1081/IMM-120027680"
}

Dimitrijević, L., Stojanović, M., Cirić, B., Radulović, M., Stojanović, R., Popović, Z., Inić-Kanada, A.,& Živković, I.. (2004). Expression of Y7 cross-reactive idiotope on human IgM molecules. in Immunological Investigations
Taylor & Francis Inc, Philadelphia., 33(1), 1-14.
https://doi.org/10.1081/IMM-120027680

Dimitrijević L, Stojanović M, Cirić B, Radulović M, Stojanović R, Popović Z, Inić-Kanada A, Živković I. Expression of Y7 cross-reactive idiotope on human IgM molecules. in Immunological Investigations. 2004;33(1):1-14.
doi:10.1081/IMM-120027680 .

Dimitrijević, Ljiljana, Stojanović, M., Cirić, B., Radulović, M., Stojanović, R., Popović, Zoran, Inić-Kanada, Aleksandra, Živković, Irena, "Expression of Y7 cross-reactive idiotope on human IgM molecules" in Immunological Investigations, 33, no. 1 (2004):1-14,
https://doi.org/10.1081/IMM-120027680 . .

TY  - JOUR
AU  - Dimitrijević, Ljiljana
AU  - Radulović, M.
AU  - Cirić, B.
AU  - Inić, Aleksandra
AU  - Apostolski, Slobodan
PY  - 1996
UR  - http://intor.torlakinstitut.com/handle/123456789/69
AB  - We have already characterized Y7 idiotype as natural idiotype by showing its expression on IgM molecules derived form cord sera. The aim of this study was to investigate the binding properties of two human monoclonal IgM antibodies isolated from the sera of patients with Waldenstrom macroglobulinaemia, which express Y7 idiotype. In order to estimate the binding repertoire of these two IgM molecules (IgM DJ and IgM RD) we tested the isolated IgM molecules and their F(ab)2 and Fab fragments over the panel of 23 different protein and non-protein antigens, nine different bacterial strains and cell membranes from neuronal and epithelial tissues. These tests were performed by means of direct and competitive ELISA, immunoblot and immunofluorescence assays. Both antibodies reacted with ssDNA, oligonucleotide fragments, and lactobacteria. IgM DJ showed reactivity with myelin associated glycoprotein (MAG) and blood vessel endothelial cells. Binding for DNA oligonucleotide fragments and lactobacteria was interrupted up to 70% with 0.5 M NaCl, which indicated that these interactions were of low affinity. Avidity dependent binding was also evidenced by use of F(ab)2 fragments. Determined characteristics such as polyreactivity which included autoantigens, low affinity and avidity dependent binding, qualified these two monoclonal IgM as natural autoantibodies. Expression of natural idiotype Y7 on two immunoglobulins M,which possessed natural autoantibody properties, indicated the connection of natural autoantibody specificity and the natural idiotype expression and suggested the involvement of network interactions in selection of malignant B cell clones.
PB  - Forefront Publishing Group
T2  - Human Antibodies and Hybridomas
T1  - Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype
IS  - 2
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_intor_69
ER  - 

@article{
author = "Dimitrijević, Ljiljana and Radulović, M. and Cirić, B. and Inić, Aleksandra and Apostolski, Slobodan",
year = "1996",
abstract = "We have already characterized Y7 idiotype as natural idiotype by showing its expression on IgM molecules derived form cord sera. The aim of this study was to investigate the binding properties of two human monoclonal IgM antibodies isolated from the sera of patients with Waldenstrom macroglobulinaemia, which express Y7 idiotype. In order to estimate the binding repertoire of these two IgM molecules (IgM DJ and IgM RD) we tested the isolated IgM molecules and their F(ab)2 and Fab fragments over the panel of 23 different protein and non-protein antigens, nine different bacterial strains and cell membranes from neuronal and epithelial tissues. These tests were performed by means of direct and competitive ELISA, immunoblot and immunofluorescence assays. Both antibodies reacted with ssDNA, oligonucleotide fragments, and lactobacteria. IgM DJ showed reactivity with myelin associated glycoprotein (MAG) and blood vessel endothelial cells. Binding for DNA oligonucleotide fragments and lactobacteria was interrupted up to 70% with 0.5 M NaCl, which indicated that these interactions were of low affinity. Avidity dependent binding was also evidenced by use of F(ab)2 fragments. Determined characteristics such as polyreactivity which included autoantigens, low affinity and avidity dependent binding, qualified these two monoclonal IgM as natural autoantibodies. Expression of natural idiotype Y7 on two immunoglobulins M,which possessed natural autoantibody properties, indicated the connection of natural autoantibody specificity and the natural idiotype expression and suggested the involvement of network interactions in selection of malignant B cell clones.",
publisher = "Forefront Publishing Group",
journal = "Human Antibodies and Hybridomas",
title = "Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype",
number = "2",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_intor_69"
}

Dimitrijević, L., Radulović, M., Cirić, B., Inić, A.,& Apostolski, S.. (1996). Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype. in Human Antibodies and Hybridomas
Forefront Publishing Group., 7(2).
https://hdl.handle.net/21.15107/rcub_intor_69

Dimitrijević L, Radulović M, Cirić B, Inić A, Apostolski S. Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype. in Human Antibodies and Hybridomas. 1996;7(2).
https://hdl.handle.net/21.15107/rcub_intor_69 .

Dimitrijević, Ljiljana, Radulović, M., Cirić, B., Inić, Aleksandra, Apostolski, Slobodan, "Paratopic specificity of two human monoclonal immunoglobulins M expressing Y7 idiotype" in Human Antibodies and Hybridomas, 7, no. 2 (1996),
https://hdl.handle.net/21.15107/rcub_intor_69 .