TY  - JOUR
AU  - Trtić, Tatjana
AU  - Vuksanović, L.
AU  - Paranos, Svetlana
AU  - Petrović, S.
AU  - Vujčić, Zoran
AU  - Plećaš, Ilija B.
AU  - Jankov, Ratko
PY  - 1996
UR  - http://intor.torlakinstitut.com/handle/123456789/64
AB  - One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Journal of Radioanalytical and Nuclear Chemistry-Articles
T1  - Development of RAST assay for determination of anti-Populus canadensis IgE antibodies
EP  - 149
IS  - 1
SP  - 145
VL  - 206
DO  - 10.1007/BF02040051
ER  - 

@article{
author = "Trtić, Tatjana and Vuksanović, L. and Paranos, Svetlana and Petrović, S. and Vujčić, Zoran and Plećaš, Ilija B. and Jankov, Ratko",
year = "1996",
abstract = "One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Journal of Radioanalytical and Nuclear Chemistry-Articles",
title = "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies",
pages = "149-145",
number = "1",
volume = "206",
doi = "10.1007/BF02040051"
}

Trtić, T., Vuksanović, L., Paranos, S., Petrović, S., Vujčić, Z., Plećaš, I. B.,& Jankov, R.. (1996). Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry-Articles
Akademiai Kiado Zrt, Budapest., 206(1), 145-149.
https://doi.org/10.1007/BF02040051

Trtić T, Vuksanović L, Paranos S, Petrović S, Vujčić Z, Plećaš IB, Jankov R. Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry-Articles. 1996;206(1):145-149.
doi:10.1007/BF02040051 .

Trtić, Tatjana, Vuksanović, L., Paranos, Svetlana, Petrović, S., Vujčić, Zoran, Plećaš, Ilija B., Jankov, Ratko, "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies" in Journal of Radioanalytical and Nuclear Chemistry-Articles, 206, no. 1 (1996):145-149,
https://doi.org/10.1007/BF02040051 . .