TY  - JOUR
AU  - Madi, Haowa
AU  - Lukić, Jovanka
AU  - Vasiljević, Zorica
AU  - Biocanin, Marjan
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Lozo, Jelena
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/923
UR  - http://intor.torlakinstitut.com/handle/123456789/745
AB  - Background Stenotrophomonas maltophilia is an environmental bacterium and an opportunistic pathogen usually associated with healthcare-associated infections, which has recently been recognized as a globally multi-drug resistant organism. The aim of this study was genotyping and physiological characterization of Stenotrophomonas maltophilia isolated in a large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national reference cystic fibrosis (CF) center for pediatric and adult patients. Methods We characterized 42 strains of cystic fibrosis (CF) and 46 strains of non-cystic fibrosis (non-CF) origin isolated from 2013 to 2015 in order to investigate their genetic relatedness and phenotypic traits. Genotyping was performed using sequencing of 16S rRNA gene, Pulse Field Gel Electrophoresis (PFGE) and Multi locus sequencing typing (MLST) analysis. Sensitivity to five relevant antimicrobial agents was determined, namely trimethoprim/sulfamethoxazole (TMP/SMX), chloramphenicol, ciprofloxacin, levofloxacin and tetracycline. Surface characteristics, motility, biofilm formation and adhesion to mucin were tested in all strains. Statistical approach was used to determine correlations between obtained results. Results Most of the isolates were not genetically related. Six new sequence types were determined. Strains were uniformly sensitive to all tested antimicrobial agents. The majority of isolates (89.8%) were able to form biofilm with almost equal representation in both CF and non-CF strains. Swimming motility was observed in all strains, while none of them exhibited swarming motility. Among strains able to adhere to mucin, no differences between CF and non-CF isolates were observed. Conclusions High genetic diversity among isolates implies the absence of clonal spread within the hospital. Positive correlation between motility, biofilm formation and adhesion to mucin was demonstrated. Biofilm formation and motility were more pronounced among non-CF than CF isolates.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia
IS  - 10
VL  - 11
DO  - 10.1371/journal.pone.0165660
ER  - 

@article{
author = "Madi, Haowa and Lukić, Jovanka and Vasiljević, Zorica and Biocanin, Marjan and Kojić, Milan and Jovčić, Branko and Lozo, Jelena",
year = "2016",
abstract = "Background Stenotrophomonas maltophilia is an environmental bacterium and an opportunistic pathogen usually associated with healthcare-associated infections, which has recently been recognized as a globally multi-drug resistant organism. The aim of this study was genotyping and physiological characterization of Stenotrophomonas maltophilia isolated in a large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national reference cystic fibrosis (CF) center for pediatric and adult patients. Methods We characterized 42 strains of cystic fibrosis (CF) and 46 strains of non-cystic fibrosis (non-CF) origin isolated from 2013 to 2015 in order to investigate their genetic relatedness and phenotypic traits. Genotyping was performed using sequencing of 16S rRNA gene, Pulse Field Gel Electrophoresis (PFGE) and Multi locus sequencing typing (MLST) analysis. Sensitivity to five relevant antimicrobial agents was determined, namely trimethoprim/sulfamethoxazole (TMP/SMX), chloramphenicol, ciprofloxacin, levofloxacin and tetracycline. Surface characteristics, motility, biofilm formation and adhesion to mucin were tested in all strains. Statistical approach was used to determine correlations between obtained results. Results Most of the isolates were not genetically related. Six new sequence types were determined. Strains were uniformly sensitive to all tested antimicrobial agents. The majority of isolates (89.8%) were able to form biofilm with almost equal representation in both CF and non-CF strains. Swimming motility was observed in all strains, while none of them exhibited swarming motility. Among strains able to adhere to mucin, no differences between CF and non-CF isolates were observed. Conclusions High genetic diversity among isolates implies the absence of clonal spread within the hospital. Positive correlation between motility, biofilm formation and adhesion to mucin was demonstrated. Biofilm formation and motility were more pronounced among non-CF than CF isolates.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia",
number = "10",
volume = "11",
doi = "10.1371/journal.pone.0165660"
}

Madi, H., Lukić, J., Vasiljević, Z., Biocanin, M., Kojić, M., Jovčić, B.,& Lozo, J.. (2016). Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia. in PLoS One
Public Library Science, San Francisco., 11(10).
https://doi.org/10.1371/journal.pone.0165660

Madi H, Lukić J, Vasiljević Z, Biocanin M, Kojić M, Jovčić B, Lozo J. Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia. in PLoS One. 2016;11(10).
doi:10.1371/journal.pone.0165660 .

Madi, Haowa, Lukić, Jovanka, Vasiljević, Zorica, Biocanin, Marjan, Kojić, Milan, Jovčić, Branko, Lozo, Jelena, "Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia" in PLoS One, 11, no. 10 (2016),
https://doi.org/10.1371/journal.pone.0165660 . .