TY  - JOUR
AU  - Aguilar, C
AU  - Friscina, A
AU  - Devescovi, G
AU  - Kojić, Milan
AU  - Venturi, V
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/798
AB  - Quorum sensing is a regulatory mechanism (operating in response to cell density) which in gram-negative bacteria usually involves the production of N-acyl homoserine lactones (HSL). Quorum sensing in Burkholderia cepacia has been associated with the regulation of expression of extracellular proteins and siderophores and also with the regulation of swarming and biofilm formation. In the present study, several quorum-sensing-controlled gene promoters of B. cepacia ATCC 25416 were identified and characterized. A total of 28 putative gene promoters show CepR-C-8-HSL-dependent expression, suggesting that quorum sensing in B. cepacia is a global regulatory system.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - Identification of quorum-sensing-regulated genes of Burkholderia cepacia
EP  - 6462
IS  - 21
SP  - 6456
VL  - 185
DO  - 10.1128/JB.185.21.6456-6462.2003
ER  - 

@article{
author = "Aguilar, C and Friscina, A and Devescovi, G and Kojić, Milan and Venturi, V",
year = "2003",
abstract = "Quorum sensing is a regulatory mechanism (operating in response to cell density) which in gram-negative bacteria usually involves the production of N-acyl homoserine lactones (HSL). Quorum sensing in Burkholderia cepacia has been associated with the regulation of expression of extracellular proteins and siderophores and also with the regulation of swarming and biofilm formation. In the present study, several quorum-sensing-controlled gene promoters of B. cepacia ATCC 25416 were identified and characterized. A total of 28 putative gene promoters show CepR-C-8-HSL-dependent expression, suggesting that quorum sensing in B. cepacia is a global regulatory system.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "Identification of quorum-sensing-regulated genes of Burkholderia cepacia",
pages = "6462-6456",
number = "21",
volume = "185",
doi = "10.1128/JB.185.21.6456-6462.2003"
}

Aguilar, C., Friscina, A., Devescovi, G., Kojić, M.,& Venturi, V.. (2003). Identification of quorum-sensing-regulated genes of Burkholderia cepacia. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 185(21), 6456-6462.
https://doi.org/10.1128/JB.185.21.6456-6462.2003

Aguilar C, Friscina A, Devescovi G, Kojić M, Venturi V. Identification of quorum-sensing-regulated genes of Burkholderia cepacia. in Journal of Bacteriology. 2003;185(21):6456-6462.
doi:10.1128/JB.185.21.6456-6462.2003 .

Aguilar, C, Friscina, A, Devescovi, G, Kojić, Milan, Venturi, V, "Identification of quorum-sensing-regulated genes of Burkholderia cepacia" in Journal of Bacteriology, 185, no. 21 (2003):6456-6462,
https://doi.org/10.1128/JB.185.21.6456-6462.2003 . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Aguilar, C
AU  - Venturi, V
PY  - 2002
UR  - http://intor.torlakinstitut.com/handle/123456789/797
AB  - We have previously described a Pseudomonas gene, psrA, which enhances transcription of the rpoS sigma factor gene at stationary phase. We present molecular data which demonstrate that in Pseudomonas putida PsrA binds specifically to the rpoS and psrA promoters in DNA regions having similar palindromic sequences, C/GAAAC N2-4 GTTTG/C, where N is any nucleotide. The position of the initiation of transcription was determined for both promoters, and PsrA binds from positions - 59 to -35 in the rpoS promoter and from - 18 to + 20 in the psrA promoter with respect to the +1 transcription site. Expression studies with a psrA-lacZ transcriptional fusion in wild-type and psrA::Tn5 knockout mutants revealed that psrA was under additional control in response to growth phase. A model for the role of PsrA in the regulation of rpoS and psrA is presented.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters
EP  - 2330
IS  - 8
SP  - 2324
VL  - 184
DO  - 10.1128/JB.184.8.2324-2330.2002
ER  - 

@article{
author = "Kojić, Milan and Aguilar, C and Venturi, V",
year = "2002",
abstract = "We have previously described a Pseudomonas gene, psrA, which enhances transcription of the rpoS sigma factor gene at stationary phase. We present molecular data which demonstrate that in Pseudomonas putida PsrA binds specifically to the rpoS and psrA promoters in DNA regions having similar palindromic sequences, C/GAAAC N2-4 GTTTG/C, where N is any nucleotide. The position of the initiation of transcription was determined for both promoters, and PsrA binds from positions - 59 to -35 in the rpoS promoter and from - 18 to + 20 in the psrA promoter with respect to the +1 transcription site. Expression studies with a psrA-lacZ transcriptional fusion in wild-type and psrA::Tn5 knockout mutants revealed that psrA was under additional control in response to growth phase. A model for the role of PsrA in the regulation of rpoS and psrA is presented.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters",
pages = "2330-2324",
number = "8",
volume = "184",
doi = "10.1128/JB.184.8.2324-2330.2002"
}

Kojić, M., Aguilar, C.,& Venturi, V.. (2002). TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 184(8), 2324-2330.
https://doi.org/10.1128/JB.184.8.2324-2330.2002

Kojić M, Aguilar C, Venturi V. TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters. in Journal of Bacteriology. 2002;184(8):2324-2330.
doi:10.1128/JB.184.8.2324-2330.2002 .

Kojić, Milan, Aguilar, C, Venturi, V, "TetR family member PsrA directly binds the Pseudomonas rpoS and psrA promoters" in Journal of Bacteriology, 184, no. 8 (2002):2324-2330,
https://doi.org/10.1128/JB.184.8.2324-2330.2002 . .