TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Stein, Elisabeth
AU  - Ladurner, Angela
AU  - Mayr, Ulrike Beate
AU  - Lubitz, Werner
AU  - Barisani-Asenbauer, Talin
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/415
AB  - Purpose: In this work we explored the efficacies of Salmonella typhimurium (ST) Bacterial Ghosts (BGs), using the conjunctival route of immunization, to induce mucosal and systemic immunity against ST.

Methods: BALB/c and C57BL/6 mice were immunized via the conjunctiva with ST BGs in three different doses (50, 25 and 12.5 μg/mouse). Three immunizations were performed at 2 weeks interval and the evaluation of local and systemic immune response was done 2 and 8 weeks after the last immunization. This research was conducted in compliance with the “ARVO Statement of the Use of Animals in Ophthalmic and Visual Research” and the “Declaration of Helsinki”.

Results: ST BGs were well tolerated; non-toxic and successfully uptaken by the mouse conjunctival cells. We have found ST BG-specific IgG and IgA in tears and sera of both mice strains, as well as, IgG positive ST BG-specific cells. It was shown that the amount of ST BG-specific antibodies in mice' sera strongly correlated with the presence of ST BG-specific B cells in draining lymph nodes. 8 weeks after the last immunization, the levels of anti-ST BG IgG and IgA in the sera of BALB/c mice were higher than in C57BL/6. BALB/c mice exhibited also more prominent anti-ST BG IgA concentration in mice’ tears. The strongest IgG and IgA response was obtained with the highest dose used for immunization (50 μg/mouse). The conjunctival application of ST BGs in both mouse strains induced skewing of ST BG-specific immune responses toward a Th1/Th17 profile, as determined by the stimulation of IFNγ and IL-17A secretion and/or the concurrent pronounced reduction of IL-4 secretion.

Conclusions: Conjunctival immunization induced a strong ST BG-specific local and systemic immune response, and a Th1-biased response that is critical for control of Salmonella species. ST BGs, non-living shells of Gram negative ST bacteria, possess excellent safety properties and could be used as a candidate vaccine for an ocular delivery.
PB  - Assoc Research Vision Ophthalmology Inc, Rockville
C3  - Investigative Ophthalmology & Visual Science
T1  - The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts
IS  - 13
VL  - 55
UR  - https://hdl.handle.net/21.15107/rcub_intor_415
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Stein, Elisabeth and Ladurner, Angela and Mayr, Ulrike Beate and Lubitz, Werner and Barisani-Asenbauer, Talin",
year = "2014",
abstract = "Purpose: In this work we explored the efficacies of Salmonella typhimurium (ST) Bacterial Ghosts (BGs), using the conjunctival route of immunization, to induce mucosal and systemic immunity against ST.

Methods: BALB/c and C57BL/6 mice were immunized via the conjunctiva with ST BGs in three different doses (50, 25 and 12.5 μg/mouse). Three immunizations were performed at 2 weeks interval and the evaluation of local and systemic immune response was done 2 and 8 weeks after the last immunization. This research was conducted in compliance with the “ARVO Statement of the Use of Animals in Ophthalmic and Visual Research” and the “Declaration of Helsinki”.

Results: ST BGs were well tolerated; non-toxic and successfully uptaken by the mouse conjunctival cells. We have found ST BG-specific IgG and IgA in tears and sera of both mice strains, as well as, IgG positive ST BG-specific cells. It was shown that the amount of ST BG-specific antibodies in mice' sera strongly correlated with the presence of ST BG-specific B cells in draining lymph nodes. 8 weeks after the last immunization, the levels of anti-ST BG IgG and IgA in the sera of BALB/c mice were higher than in C57BL/6. BALB/c mice exhibited also more prominent anti-ST BG IgA concentration in mice’ tears. The strongest IgG and IgA response was obtained with the highest dose used for immunization (50 μg/mouse). The conjunctival application of ST BGs in both mouse strains induced skewing of ST BG-specific immune responses toward a Th1/Th17 profile, as determined by the stimulation of IFNγ and IL-17A secretion and/or the concurrent pronounced reduction of IL-4 secretion.

Conclusions: Conjunctival immunization induced a strong ST BG-specific local and systemic immune response, and a Th1-biased response that is critical for control of Salmonella species. ST BGs, non-living shells of Gram negative ST bacteria, possess excellent safety properties and could be used as a candidate vaccine for an ocular delivery.",
publisher = "Assoc Research Vision Ophthalmology Inc, Rockville",
journal = "Investigative Ophthalmology & Visual Science",
title = "The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts",
number = "13",
volume = "55",
url = "https://hdl.handle.net/21.15107/rcub_intor_415"
}

Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Stein, E., Ladurner, A., Mayr, U. B., Lubitz, W.,& Barisani-Asenbauer, T.. (2014). The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts. in Investigative Ophthalmology & Visual Science
Assoc Research Vision Ophthalmology Inc, Rockville., 55(13).
https://hdl.handle.net/21.15107/rcub_intor_415

Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Stein E, Ladurner A, Mayr UB, Lubitz W, Barisani-Asenbauer T. The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts. in Investigative Ophthalmology & Visual Science. 2014;55(13).
https://hdl.handle.net/21.15107/rcub_intor_415 .

Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Stein, Elisabeth, Ladurner, Angela, Mayr, Ulrike Beate, Lubitz, Werner, Barisani-Asenbauer, Talin, "The ocular conjunctiva and conjunctiva-associated lymphoid tissue as a mucosal immunization route: humoral and cellular immune responses against Salmonella typhimurium Bacterial Ghosts" in Investigative Ophthalmology & Visual Science, 55, no. 13 (2014),
https://hdl.handle.net/21.15107/rcub_intor_415 .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Montanaro, Jacqueline
AU  - Stein, Elisabeth
AU  - Bintner, Nora
AU  - Schuerer, Nadine
AU  - Ladurner, Angela
AU  - Barisani-Asenbauer, Talin
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/404
PB  - Wiley-Blackwell, Hoboken
C3  - Immunology
T1  - Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis
EP  - 25
SP  - 25
VL  - 143
UR  - https://hdl.handle.net/21.15107/rcub_intor_404
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Montanaro, Jacqueline and Stein, Elisabeth and Bintner, Nora and Schuerer, Nadine and Ladurner, Angela and Barisani-Asenbauer, Talin",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Immunology",
title = "Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis",
pages = "25-25",
volume = "143",
url = "https://hdl.handle.net/21.15107/rcub_intor_404"
}

Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Montanaro, J., Stein, E., Bintner, N., Schuerer, N., Ladurner, A.,& Barisani-Asenbauer, T.. (2014). Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis. in Immunology
Wiley-Blackwell, Hoboken., 143, 25-25.
https://hdl.handle.net/21.15107/rcub_intor_404

Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Montanaro J, Stein E, Bintner N, Schuerer N, Ladurner A, Barisani-Asenbauer T. Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis. in Immunology. 2014;143:25-25.
https://hdl.handle.net/21.15107/rcub_intor_404 .

Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Montanaro, Jacqueline, Stein, Elisabeth, Bintner, Nora, Schuerer, Nadine, Ladurner, Angela, Barisani-Asenbauer, Talin, "Immunization via conjunctiva and CALT as a route for chlamydia vaccine delivery: Immune responses to PmpC, an outer membrane protein of C. trachomatis" in Immunology, 143 (2014):25-25,
https://hdl.handle.net/21.15107/rcub_intor_404 .

TY  - JOUR
AU  - Stojanović, Marijana
AU  - Petrušić, Vladimir
AU  - Živković, Irena
AU  - Inić-Kanada, Aleksandra
AU  - Stojičević, Ivana
AU  - Marinković, Emilija
AU  - Dimitrijević, Ljiljana
PY  - 2013
UR  - http://intor.torlakinstitut.com/handle/123456789/395
AB  - It is known that tetanus toxoid (TTd)-hyperimmunization induces increased titer of sera beta 2-glycoprotein I (beta 2GPI)-specific antibodies (Abs) in Balb/c mice. The concentrations of such induced anti-beta 2GPI Abs as well as their pathogenic potential are strongly influenced by the context of TTd application. beta 2GPI-specific immune response is established as a part of TTd-specific immune response by molecular mimicry mechanism due to structural homology between TTd and beta 2GPI. This finding is supported by the following facts: (1) cross-reactive Abs that recognize both TTd and beta 2GPI epitopes are present in Balb/c mice sera; (2) anti-TTd Abs secretion in splenic cultures is induced after beta 2GPI stimulation and vice versa. However, analyses of (1) IL-10 production following in vitro stimulation of immunized Balb/c mice splenocytes by TTd, beta 2GPI or glutaraldehyde-treated beta 2GPI and (2) specific impact of ConA and agonists of TLR2, TLR4, and TLR9 on anti-TTd and autoreactive Abs secretion strongly imply that these two branches of the TTd-induced immune response do not use identical cell populations and are regulated in a different way. Results presented in this paper describe that structural homology between foreign and self-antigens could focus mounted autoreactive immune response toward specific self-structure, but the context of antigen application, including a history of previous immune stimulations and adjuvants applied together with the antigen, are the main factors which determine the outcome of the induced immune response.
PB  - Humana Press Inc, Totowa
T2  - Immunologic Research
T1  - Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid
EP  - 31
IS  - 1
SP  - 20
VL  - 56
DO  - 10.1007/s12026-012-8343-1
ER  - 

@article{
author = "Stojanović, Marijana and Petrušić, Vladimir and Živković, Irena and Inić-Kanada, Aleksandra and Stojičević, Ivana and Marinković, Emilija and Dimitrijević, Ljiljana",
year = "2013",
abstract = "It is known that tetanus toxoid (TTd)-hyperimmunization induces increased titer of sera beta 2-glycoprotein I (beta 2GPI)-specific antibodies (Abs) in Balb/c mice. The concentrations of such induced anti-beta 2GPI Abs as well as their pathogenic potential are strongly influenced by the context of TTd application. beta 2GPI-specific immune response is established as a part of TTd-specific immune response by molecular mimicry mechanism due to structural homology between TTd and beta 2GPI. This finding is supported by the following facts: (1) cross-reactive Abs that recognize both TTd and beta 2GPI epitopes are present in Balb/c mice sera; (2) anti-TTd Abs secretion in splenic cultures is induced after beta 2GPI stimulation and vice versa. However, analyses of (1) IL-10 production following in vitro stimulation of immunized Balb/c mice splenocytes by TTd, beta 2GPI or glutaraldehyde-treated beta 2GPI and (2) specific impact of ConA and agonists of TLR2, TLR4, and TLR9 on anti-TTd and autoreactive Abs secretion strongly imply that these two branches of the TTd-induced immune response do not use identical cell populations and are regulated in a different way. Results presented in this paper describe that structural homology between foreign and self-antigens could focus mounted autoreactive immune response toward specific self-structure, but the context of antigen application, including a history of previous immune stimulations and adjuvants applied together with the antigen, are the main factors which determine the outcome of the induced immune response.",
publisher = "Humana Press Inc, Totowa",
journal = "Immunologic Research",
title = "Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid",
pages = "31-20",
number = "1",
volume = "56",
doi = "10.1007/s12026-012-8343-1"
}

Stojanović, M., Petrušić, V., Živković, I., Inić-Kanada, A., Stojičević, I., Marinković, E.,& Dimitrijević, L.. (2013). Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid. in Immunologic Research
Humana Press Inc, Totowa., 56(1), 20-31.
https://doi.org/10.1007/s12026-012-8343-1

Stojanović M, Petrušić V, Živković I, Inić-Kanada A, Stojičević I, Marinković E, Dimitrijević L. Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid. in Immunologic Research. 2013;56(1):20-31.
doi:10.1007/s12026-012-8343-1 .

Stojanović, Marijana, Petrušić, Vladimir, Živković, Irena, Inić-Kanada, Aleksandra, Stojičević, Ivana, Marinković, Emilija, Dimitrijević, Ljiljana, "Role of molecular mimicry and polyclonal cell activation in the induction of pathogenic beta 2-glycoprotein I-directed immune response in Balb/c mice upon hyperimmunization with tetanus toxoid" in Immunologic Research, 56, no. 1 (2013):20-31,
https://doi.org/10.1007/s12026-012-8343-1 . .

TY  - CONF
AU  - Inić-Kanada, Aleksandra
AU  - Belij, Sandra
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Stojičević, Ivana
AU  - Montanaro, Jacqueline
AU  - Schlacher, Simone
AU  - Stein, Elisabeth
AU  - Bintner, Nora
AU  - Barisani-Asenbauer, Talin
PY  - 2012
UR  - http://intor.torlakinstitut.com/handle/123456789/341
PB  - Wiley-Blackwell, Hoboken
C3  - Immunology
T1  - Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen
EP  - 767
SP  - 767
VL  - 137
UR  - https://hdl.handle.net/21.15107/rcub_intor_341
ER  - 

@conference{
author = "Inić-Kanada, Aleksandra and Belij, Sandra and Stojanović, Marijana and Marinković, Emilija and Stojičević, Ivana and Montanaro, Jacqueline and Schlacher, Simone and Stein, Elisabeth and Bintner, Nora and Barisani-Asenbauer, Talin",
year = "2012",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Immunology",
title = "Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen",
pages = "767-767",
volume = "137",
url = "https://hdl.handle.net/21.15107/rcub_intor_341"
}

Inić-Kanada, A., Belij, S., Stojanović, M., Marinković, E., Stojičević, I., Montanaro, J., Schlacher, S., Stein, E., Bintner, N.,& Barisani-Asenbauer, T.. (2012). Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen. in Immunology
Wiley-Blackwell, Hoboken., 137, 767-767.
https://hdl.handle.net/21.15107/rcub_intor_341

Inić-Kanada A, Belij S, Stojanović M, Marinković E, Stojičević I, Montanaro J, Schlacher S, Stein E, Bintner N, Barisani-Asenbauer T. Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen. in Immunology. 2012;137:767-767.
https://hdl.handle.net/21.15107/rcub_intor_341 .

Inić-Kanada, Aleksandra, Belij, Sandra, Stojanović, Marijana, Marinković, Emilija, Stojičević, Ivana, Montanaro, Jacqueline, Schlacher, Simone, Stein, Elisabeth, Bintner, Nora, Barisani-Asenbauer, Talin, "Novel vaccine strategy for ocular surface infections using conjunctiva-associated lymphoid tissue as a route of immunization: local and systemic responses to model antigen" in Immunology, 137 (2012):767-767,
https://hdl.handle.net/21.15107/rcub_intor_341 .