TY  - JOUR
AU  - Gavrović-Jankulović, Marija
AU  - Ćirković, Tanja
AU  - Bukilica, Mirjana
AU  - Fahlbusch, B.
AU  - Petrović, Spomenka
AU  - Jankov, Ratko
PY  - 2000
UR  - http://intor.torlakinstitut.com/handle/123456789/116
AB  - More than 75% of grass pollen-allergic patients produce specific IgE antibodies against group-4 allergens. Purification and characterization of different grass group-4 allergens should help to further understand their allergenicity. In this study, an attempt was made to isolate and characterize Fes p 4 allergen by several biochemical and immunochemical methods. Fes p 4 was purified by a combination of chromatographic techniques (gel permeation and ion exchange chromatography). Isolated protein revealed four main spots at a molecular weight of 60 kDa and a pl ranging from 8.7 to 9.1. Eight sera were selected from patients with positive result of skin prick test to the mixture of grass pollen extracts. ELISA inhibition technique was used to study Fes p 4-specific IgE in the patients' sera. ELISA to Festuca pratensis was inhibited up to 80% by F. pratensis pollen extract and up to 48% by Fes p 4. 2D-PAGE-immunoblot was used to identify allergenic and antigenic components of Fes p 4 with patients' IgE and monoclonal antibodies (MABs). Three components of purified protein expressed IgE binding ability. Two MABs which recognized unrelated regions on Phl p 4, bound three components of Fes p 4. The role of the carbohydrate moiety in allergenicity was examined with individual patient sera by using periodate-treated Fes p 4. Six out of eight patients reduced IgE binding to periodate-treated allergen. Isolated Fes p 4 glycoprotein consisted of four components, three of which were allergenic, and share common epitopes specific for grass group-4 homologs. The results of periodate oxidation of Fes p 4 suggest that the carbohydrate moiety is involved in IgE binding.
T2  - Journal of Investigational Allergology and Clinical Immunology
T1  - Isolation and partial characterization of Fes p 4 allergen
EP  - 367
IS  - 6
SP  - 361
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_intor_116
ER  - 

@article{
author = "Gavrović-Jankulović, Marija and Ćirković, Tanja and Bukilica, Mirjana and Fahlbusch, B. and Petrović, Spomenka and Jankov, Ratko",
year = "2000",
abstract = "More than 75% of grass pollen-allergic patients produce specific IgE antibodies against group-4 allergens. Purification and characterization of different grass group-4 allergens should help to further understand their allergenicity. In this study, an attempt was made to isolate and characterize Fes p 4 allergen by several biochemical and immunochemical methods. Fes p 4 was purified by a combination of chromatographic techniques (gel permeation and ion exchange chromatography). Isolated protein revealed four main spots at a molecular weight of 60 kDa and a pl ranging from 8.7 to 9.1. Eight sera were selected from patients with positive result of skin prick test to the mixture of grass pollen extracts. ELISA inhibition technique was used to study Fes p 4-specific IgE in the patients' sera. ELISA to Festuca pratensis was inhibited up to 80% by F. pratensis pollen extract and up to 48% by Fes p 4. 2D-PAGE-immunoblot was used to identify allergenic and antigenic components of Fes p 4 with patients' IgE and monoclonal antibodies (MABs). Three components of purified protein expressed IgE binding ability. Two MABs which recognized unrelated regions on Phl p 4, bound three components of Fes p 4. The role of the carbohydrate moiety in allergenicity was examined with individual patient sera by using periodate-treated Fes p 4. Six out of eight patients reduced IgE binding to periodate-treated allergen. Isolated Fes p 4 glycoprotein consisted of four components, three of which were allergenic, and share common epitopes specific for grass group-4 homologs. The results of periodate oxidation of Fes p 4 suggest that the carbohydrate moiety is involved in IgE binding.",
journal = "Journal of Investigational Allergology and Clinical Immunology",
title = "Isolation and partial characterization of Fes p 4 allergen",
pages = "367-361",
number = "6",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_intor_116"
}

Gavrović-Jankulović, M., Ćirković, T., Bukilica, M., Fahlbusch, B., Petrović, S.,& Jankov, R.. (2000). Isolation and partial characterization of Fes p 4 allergen. in Journal of Investigational Allergology and Clinical Immunology, 10(6), 361-367.
https://hdl.handle.net/21.15107/rcub_intor_116

Gavrović-Jankulović M, Ćirković T, Bukilica M, Fahlbusch B, Petrović S, Jankov R. Isolation and partial characterization of Fes p 4 allergen. in Journal of Investigational Allergology and Clinical Immunology. 2000;10(6):361-367.
https://hdl.handle.net/21.15107/rcub_intor_116 .

Gavrović-Jankulović, Marija, Ćirković, Tanja, Bukilica, Mirjana, Fahlbusch, B., Petrović, Spomenka, Jankov, Ratko, "Isolation and partial characterization of Fes p 4 allergen" in Journal of Investigational Allergology and Clinical Immunology, 10, no. 6 (2000):361-367,
https://hdl.handle.net/21.15107/rcub_intor_116 .

TY  - CONF
AU  - Đorđević, I.L.
AU  - Marković, B.M.
AU  - Lazarević, M.
AU  - Bukilica, Mirjana
AU  - Đurić, V.J.
AU  - Janković, B.D.
PY  - 1990
UR  - http://intor.torlakinstitut.com/handle/123456789/39
PB  - Hrvatsko prirodoslovno društvo, Zagreb
C3  - Periodicum Biologorum
T1  - Acute stress and anaphylactic shock in the rat
EP  - 79
IS  - 1
SP  - 78
VL  - 92
UR  - https://hdl.handle.net/21.15107/rcub_intor_39
ER  - 

@conference{
author = "Đorđević, I.L. and Marković, B.M. and Lazarević, M. and Bukilica, Mirjana and Đurić, V.J. and Janković, B.D.",
year = "1990",
publisher = "Hrvatsko prirodoslovno društvo, Zagreb",
journal = "Periodicum Biologorum",
title = "Acute stress and anaphylactic shock in the rat",
pages = "79-78",
number = "1",
volume = "92",
url = "https://hdl.handle.net/21.15107/rcub_intor_39"
}

Đorđević, I.L., Marković, B.M., Lazarević, M., Bukilica, M., Đurić, V.J.,& Janković, B.D.. (1990). Acute stress and anaphylactic shock in the rat. in Periodicum Biologorum
Hrvatsko prirodoslovno društvo, Zagreb., 92(1), 78-79.
https://hdl.handle.net/21.15107/rcub_intor_39

Đorđević I, Marković B, Lazarević M, Bukilica M, Đurić V, Janković B. Acute stress and anaphylactic shock in the rat. in Periodicum Biologorum. 1990;92(1):78-79.
https://hdl.handle.net/21.15107/rcub_intor_39 .

Đorđević, I.L., Marković, B.M., Lazarević, M., Bukilica, Mirjana, Đurić, V.J., Janković, B.D., "Acute stress and anaphylactic shock in the rat" in Periodicum Biologorum, 92, no. 1 (1990):78-79,
https://hdl.handle.net/21.15107/rcub_intor_39 .