TY  - CONF
AU  - Ćirković, Valentina
AU  - Stamenković, Gorana
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stanojević, Maja
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/533
PB  - Elsevier Sci Ltd, Oxford
C3  - International Journal of Infectious Diseases
T1  - Tula virus phylogeography
EP  - 121
SP  - 121
VL  - 79
DO  - 10.1016/j.ijid.2018.11.297
ER  - 

@conference{
author = "Ćirković, Valentina and Stamenković, Gorana and Siljić, Marina and Gligić, Ana and Stanojević, Maja",
year = "2019",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Journal of Infectious Diseases",
title = "Tula virus phylogeography",
pages = "121-121",
volume = "79",
doi = "10.1016/j.ijid.2018.11.297"
}

Ćirković, V., Stamenković, G., Siljić, M., Gligić, A.,& Stanojević, M.. (2019). Tula virus phylogeography. in International Journal of Infectious Diseases
Elsevier Sci Ltd, Oxford., 79, 121-121.
https://doi.org/10.1016/j.ijid.2018.11.297

Ćirković V, Stamenković G, Siljić M, Gligić A, Stanojević M. Tula virus phylogeography. in International Journal of Infectious Diseases. 2019;79:121-121.
doi:10.1016/j.ijid.2018.11.297 .

Ćirković, Valentina, Stamenković, Gorana, Siljić, Marina, Gligić, Ana, Stanojević, Maja, "Tula virus phylogeography" in International Journal of Infectious Diseases, 79 (2019):121-121,
https://doi.org/10.1016/j.ijid.2018.11.297 . .

TY  - JOUR
AU  - Stanojević, Maja
AU  - Ćirković, Valentina
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stamenković, Gorana
PY  - 2019
UR  - http://intor.torlakinstitut.com/handle/123456789/530
AB  - Background: Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. Methods: Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral anti-bodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. Results: PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. Conclusions: Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation. (C) 2019 Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
PB  - Elsevier Science London, London
T2  - Journal of Infection and Public Health
T1  - Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011
EP  - 649
IS  - 5
SP  - 645
VL  - 12
DO  - 10.1016/j.jiph.2019.02.021
ER  - 

@article{
author = "Stanojević, Maja and Ćirković, Valentina and Siljić, Marina and Gligić, Ana and Stamenković, Gorana",
year = "2019",
abstract = "Background: Hantaviruses are etiological agents of emerging zoonotic diseases worldwide, including hemorrhagic fever with renal syndrome (HFRS). A number of hantavirus species is known to be present in Europe. In Serbia, existing data on hantavirus presence and prevalence rely in serological findings. In this study, molecular analysis was performed in order to characterize HFRS causing hantaviruses in Serbia. Methods: Sixty four serum samples of HFRS cases, previously found seropositive to anti-hantaviral anti-bodies, were included in the study. Partial hantaviral L and S segments were PCR amplified producing 390nt and 598nt amplicons, respectively, in parallel with human beta-actin mRNA as external reverse transcription positive control. Hantavirus specific PCR products were DNA sequenced in both direction and the obtained sequences phylogenetically confirmed and analyzed. Results: PCR detection of hantavirus L and S genome segments was positive in 18/64 and 11/64 tested samples, respectively. Positive PCR results involved samples obtained from different locations, mostly from central and southern parts of Serbia. All the obtained sequences were identified as Dobrava-Belgrade virus (DOBV). In the phylogenetic analysis sequences from Serbia tended to cluster in distinctive, geographically related clusters. Conclusions: Our findings indicate DOBV as the main HFRS causing hantavirus in Serbia, the site of its initial isolation. (C) 2019 Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).",
publisher = "Elsevier Science London, London",
journal = "Journal of Infection and Public Health",
title = "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011",
pages = "649-645",
number = "5",
volume = "12",
doi = "10.1016/j.jiph.2019.02.021"
}

Stanojević, M., Ćirković, V., Siljić, M., Gligić, A.,& Stamenković, G.. (2019). Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011. in Journal of Infection and Public Health
Elsevier Science London, London., 12(5), 645-649.
https://doi.org/10.1016/j.jiph.2019.02.021

Stanojević M, Ćirković V, Siljić M, Gligić A, Stamenković G. Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011. in Journal of Infection and Public Health. 2019;12(5):645-649.
doi:10.1016/j.jiph.2019.02.021 .

Stanojević, Maja, Ćirković, Valentina, Siljić, Marina, Gligić, Ana, Stamenković, Gorana, "Molecular characterization of Dobrava-Belgrade hantavirus in Serbia, 2007-2011" in Journal of Infection and Public Health, 12, no. 5 (2019):645-649,
https://doi.org/10.1016/j.jiph.2019.02.021 . .

TY  - JOUR
AU  - Nikolić, Valentina
AU  - Stajković, Novica
AU  - Stamenković, Gorana
AU  - Čekanac, Radovan
AU  - Marušić, Predrag
AU  - Siljić, Marina
AU  - Gligić, Ana
AU  - Stanojević, Maja
PY  - 2014
UR  - http://intor.torlakinstitut.com/handle/123456789/414
AB  - Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.
PB  - Elsevier, Amsterdam
T2  - Infection Genetics and Evolution
T1  - Evidence of recombination in Tula virus strains from Serbia
EP  - 478
SP  - 472
VL  - 21
DO  - 10.1016/j.meegid.2013.08.020
ER  - 

@article{
author = "Nikolić, Valentina and Stajković, Novica and Stamenković, Gorana and Čekanac, Radovan and Marušić, Predrag and Siljić, Marina and Gligić, Ana and Stanojević, Maja",
year = "2014",
abstract = "Tula hantavirus (TULV) belongs to Bunyaviridae family, with negative sense RNA genome. Segmented nature of hantaviral genome allows for genetic reassortment, but the evidence of homologous recombination also exists. In this study we analyzed TULV sequences isolated in Serbia on different occasions and from different rodent hosts: 1987 strain from Microtus subterraneus and 2007 strain from Microtus arvalis. Phylogenetic analysis of both L and S segment sequences is suggestive of geographically related clustering, as previously shown for majority of hantaviruses. Reconstruction of phylogenetic tree for TULV S segment showed that both sequences from Serbia clustered together with sequences from East Slovakia, which had previously been shown to be recombinants (Kosice strain). Exploratory recombination analysis, supported by phylogenetic and amino acid pattern analysis, revealed the presence of recombination in the S segment sequences from Serbia, resulting in mosaic-like structure of TULV S segment similar to the one of Kosice strain. Although recombination is considered a rare event in molecular evolution of negative strand RNA viruses, obtained molecular data in this study support evidence of recombination in TULV, in geographically distant regions of Europe. (C) 2013 Elsevier B.V. All rights reserved.",
publisher = "Elsevier, Amsterdam",
journal = "Infection Genetics and Evolution",
title = "Evidence of recombination in Tula virus strains from Serbia",
pages = "478-472",
volume = "21",
doi = "10.1016/j.meegid.2013.08.020"
}

Nikolić, V., Stajković, N., Stamenković, G., Čekanac, R., Marušić, P., Siljić, M., Gligić, A.,& Stanojević, M.. (2014). Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution
Elsevier, Amsterdam., 21, 472-478.
https://doi.org/10.1016/j.meegid.2013.08.020

Nikolić V, Stajković N, Stamenković G, Čekanac R, Marušić P, Siljić M, Gligić A, Stanojević M. Evidence of recombination in Tula virus strains from Serbia. in Infection Genetics and Evolution. 2014;21:472-478.
doi:10.1016/j.meegid.2013.08.020 .

Nikolić, Valentina, Stajković, Novica, Stamenković, Gorana, Čekanac, Radovan, Marušić, Predrag, Siljić, Marina, Gligić, Ana, Stanojević, Maja, "Evidence of recombination in Tula virus strains from Serbia" in Infection Genetics and Evolution, 21 (2014):472-478,
https://doi.org/10.1016/j.meegid.2013.08.020 . .