TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Rauški, Aleksandra
AU  - Kosec, Duško
AU  - Radojević, Katarina
AU  - Pilipović, Ivan
AU  - Leposavić, Gordana
PY  - 2009
UR  - http://intor.torlakinstitut.com/handle/123456789/282
AB  - A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009
PB  - Sage Publications Ltd, London
T2  - Experimental Biology and Medicine
T1  - Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats
EP  - 1074
IS  - 9
SP  - 1067
VL  - 234
DO  - 10.3181/0902-RM-63
ER  - 

@article{
author = "Stojić-Vukanić, Zorica and Rauški, Aleksandra and Kosec, Duško and Radojević, Katarina and Pilipović, Ivan and Leposavić, Gordana",
year = "2009",
abstract = "A number of different experimental approaches have been used to elucidate the impact: of basal levels of adrenal gland-derived glucocorticoids (GCs) on T cell development, and thereby T cell-mediated immune responses. However, the relevance of the adrenal GCs to T cell development is still far from clear. This study was undertaken to explore the relevance of basal levels of GCs to T cell differentiation/maturation. Eight days post-adrenalectomy in adult male rats the thymocyte yield, apoptotic and proliferative rate and the relationship amongst major thymocyte subsets, as defined by TCR alpha beta/CD4/CD8 expression, were examined using flow cytometry. Adrenal GC deprivation decreased thymocyte apoptosis and altered the kinetics of T cell differentiation/maturation. In the adrenalectomized rats there was increased thymic hypercellularity and an over-representation of the CD4+CD8+ double positive (DP) TCR alpha beta(low) cells entering selection, as well as increased numbers of their DP TCR alpha beta(-) immediate precursors. These changes were accompanied with under-representation of the postselected DP TCR alpha beta(high) and the most mature CD4-CD8+ and, particularly, CD4+CD8-single positive (SP) TCR alpha beta(high) cells. This data suggests that withdrawal of adrenal GCs produces alterations in the thymocyte selection processes, possibly affecting the diversity of functional T cell repertoire and generation of potentially self-reactive cells as indicated by the reduced proportion and number of CD4-CD8- double negative TCR alpha beta(high) cells. In addition, it indicates that GCs influence the post-selection maturation of thymocytes and plays a regulatory role in controlling the ratio of mature CD4+CD8-/CD4-CD8+ SP TCR alpha beta(high) cells. Exp Biol Med 234:1067-1074, 2009",
publisher = "Sage Publications Ltd, London",
journal = "Experimental Biology and Medicine",
title = "Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats",
pages = "1074-1067",
number = "9",
volume = "234",
doi = "10.3181/0902-RM-63"
}

Stojić-Vukanić, Z., Rauški, A., Kosec, D., Radojević, K., Pilipović, I.,& Leposavić, G.. (2009). Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats. in Experimental Biology and Medicine
Sage Publications Ltd, London., 234(9), 1067-1074.
https://doi.org/10.3181/0902-RM-63

Stojić-Vukanić Z, Rauški A, Kosec D, Radojević K, Pilipović I, Leposavić G. Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats. in Experimental Biology and Medicine. 2009;234(9):1067-1074.
doi:10.3181/0902-RM-63 .

Stojić-Vukanić, Zorica, Rauški, Aleksandra, Kosec, Duško, Radojević, Katarina, Pilipović, Ivan, Leposavić, Gordana, "Dysregulation of T-Cell Development in Adrenal Glucocorticoid-Deprived Rats" in Experimental Biology and Medicine, 234, no. 9 (2009):1067-1074,
https://doi.org/10.3181/0902-RM-63 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Rauški, Aleksandra
AU  - Radojević, Katarina
AU  - Kosec, Duško
AU  - Stanojević, Stanislava
AU  - Pilipović, Ivan
AU  - Leposavić, Gordana
PY  - 2007
UR  - http://intor.torlakinstitut.com/handle/123456789/230
AB  - As glucocorticoids influence both catecholamine synthesis and adrenoceptor expression by immune cells, the current study was undertaken to distinguish their direct effects on the development of experimental allergic encephalomyelitis from those induced by alteration of catecholamine signaling. We examined the influence of 16-day-long beta-adrenoceptor blockade with propranolol (0.40 mg/100 g body weight/day, s.c.) beginning 3 days before immunization on the development of experimental allergic encephalomyelitis in adrenalectomized (7 days before immunization) and in non-operated male Dark Agouti rats. Adrenalectomy aggravated the clinical course of experimental allergic encephalomyelitis. In contrast, propranolol attenuated both the clinical signs of the disease and decreased the number of lesions in the spinal cord. Furthermore, propranolol prevented adrenalectomy-induced aggravation of the disease course without affecting mortality. We also found that the percentage of CD4(+)CD25(+) T lymphocytes (recently activated or regulatory cells) was increased in peripheral blood of experimental allergic encephalomyelitis rats over that in the corresponding non-immunized and bovine serum albumin immunized rats. However, the percentage of these cells was reduced in adrenalectomized and/or propranolol-treated experimental allergic encephalomyelitis rats compared to control experimental allergic encephalomyelitis rats. Our findings, coupled with the clinical course of the disease and the underlying pathomorphological changes, clearly suggest that differential mechanisms were responsible for the changes in the percentage of CD4(+)CD25(+) T lymphocytes in propranolol-treated adrenalectomized rats and only propranolol-treated rats with experimental allergic encephalomyelitis. Our results, when viewed globally, indicate that: i) beta-adrenoceptor-dependent mechanisms are involved in the immunopathogenesis of experimental allergic encephalomyelitis, ii) experimental allergic encephalomyelitis has a more severe course in adrenalectomized rats and iii) beta-adrenoceptor-mediated mechanisms operate in adrenalectomy-induced aggravation of the disease. (c) 2007 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - European Journal of Pharmacology
T1  - Beta-adrenoceptor blockade ameliorates the clinical course of experimental allergic encephalomyelitis and diminishes its aggravation in adrenalectomized rats
EP  - 182
IS  - 1-3
SP  - 170
VL  - 577
DO  - 10.1016/j.ejphar.2007.08.021
ER  - 

@article{
author = "Dimitrijević, Mirjana and Rauški, Aleksandra and Radojević, Katarina and Kosec, Duško and Stanojević, Stanislava and Pilipović, Ivan and Leposavić, Gordana",
year = "2007",
abstract = "As glucocorticoids influence both catecholamine synthesis and adrenoceptor expression by immune cells, the current study was undertaken to distinguish their direct effects on the development of experimental allergic encephalomyelitis from those induced by alteration of catecholamine signaling. We examined the influence of 16-day-long beta-adrenoceptor blockade with propranolol (0.40 mg/100 g body weight/day, s.c.) beginning 3 days before immunization on the development of experimental allergic encephalomyelitis in adrenalectomized (7 days before immunization) and in non-operated male Dark Agouti rats. Adrenalectomy aggravated the clinical course of experimental allergic encephalomyelitis. In contrast, propranolol attenuated both the clinical signs of the disease and decreased the number of lesions in the spinal cord. Furthermore, propranolol prevented adrenalectomy-induced aggravation of the disease course without affecting mortality. We also found that the percentage of CD4(+)CD25(+) T lymphocytes (recently activated or regulatory cells) was increased in peripheral blood of experimental allergic encephalomyelitis rats over that in the corresponding non-immunized and bovine serum albumin immunized rats. However, the percentage of these cells was reduced in adrenalectomized and/or propranolol-treated experimental allergic encephalomyelitis rats compared to control experimental allergic encephalomyelitis rats. Our findings, coupled with the clinical course of the disease and the underlying pathomorphological changes, clearly suggest that differential mechanisms were responsible for the changes in the percentage of CD4(+)CD25(+) T lymphocytes in propranolol-treated adrenalectomized rats and only propranolol-treated rats with experimental allergic encephalomyelitis. Our results, when viewed globally, indicate that: i) beta-adrenoceptor-dependent mechanisms are involved in the immunopathogenesis of experimental allergic encephalomyelitis, ii) experimental allergic encephalomyelitis has a more severe course in adrenalectomized rats and iii) beta-adrenoceptor-mediated mechanisms operate in adrenalectomy-induced aggravation of the disease. (c) 2007 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "European Journal of Pharmacology",
title = "Beta-adrenoceptor blockade ameliorates the clinical course of experimental allergic encephalomyelitis and diminishes its aggravation in adrenalectomized rats",
pages = "182-170",
number = "1-3",
volume = "577",
doi = "10.1016/j.ejphar.2007.08.021"
}

Dimitrijević, M., Rauški, A., Radojević, K., Kosec, D., Stanojević, S., Pilipović, I.,& Leposavić, G.. (2007). Beta-adrenoceptor blockade ameliorates the clinical course of experimental allergic encephalomyelitis and diminishes its aggravation in adrenalectomized rats. in European Journal of Pharmacology
Elsevier Science Bv, Amsterdam., 577(1-3), 170-182.
https://doi.org/10.1016/j.ejphar.2007.08.021

Dimitrijević M, Rauški A, Radojević K, Kosec D, Stanojević S, Pilipović I, Leposavić G. Beta-adrenoceptor blockade ameliorates the clinical course of experimental allergic encephalomyelitis and diminishes its aggravation in adrenalectomized rats. in European Journal of Pharmacology. 2007;577(1-3):170-182.
doi:10.1016/j.ejphar.2007.08.021 .

Dimitrijević, Mirjana, Rauški, Aleksandra, Radojević, Katarina, Kosec, Duško, Stanojević, Stanislava, Pilipović, Ivan, Leposavić, Gordana, "Beta-adrenoceptor blockade ameliorates the clinical course of experimental allergic encephalomyelitis and diminishes its aggravation in adrenalectomized rats" in European Journal of Pharmacology, 577, no. 1-3 (2007):170-182,
https://doi.org/10.1016/j.ejphar.2007.08.021 . .

TY  - CONF
AU  - Rauški, Aleksandra
AU  - Kosec, Duško
AU  - Leposavić, Gordana
PY  - 2004
UR  - http://intor.torlakinstitut.com/handle/123456789/180
PB  - Elsevier, Amsterdam
C3  - Journal of Neuroimmunology
T1  - Catecholamines influence development of EAE
EP  - 86
IS  - 1-2
SP  - 86
VL  - 154
UR  - https://hdl.handle.net/21.15107/rcub_intor_180
ER  - 

@conference{
author = "Rauški, Aleksandra and Kosec, Duško and Leposavić, Gordana",
year = "2004",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Catecholamines influence development of EAE",
pages = "86-86",
number = "1-2",
volume = "154",
url = "https://hdl.handle.net/21.15107/rcub_intor_180"
}

Rauški, A., Kosec, D.,& Leposavić, G.. (2004). Catecholamines influence development of EAE. in Journal of Neuroimmunology
Elsevier, Amsterdam., 154(1-2), 86-86.
https://hdl.handle.net/21.15107/rcub_intor_180

Rauški A, Kosec D, Leposavić G. Catecholamines influence development of EAE. in Journal of Neuroimmunology. 2004;154(1-2):86-86.
https://hdl.handle.net/21.15107/rcub_intor_180 .

Rauški, Aleksandra, Kosec, Duško, Leposavić, Gordana, "Catecholamines influence development of EAE" in Journal of Neuroimmunology, 154, no. 1-2 (2004):86-86,
https://hdl.handle.net/21.15107/rcub_intor_180 .

TY  - JOUR
AU  - Rauški, Aleksandra
AU  - Kosec, Duško
AU  - Vidić-Danković, Biljana
AU  - Plećaš-Solarović, Bosiljka
AU  - Leposavić, Gordana
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/162
AB  - The study revealed that beta-adrenoceptor blockade with propranolol (0.40 mg/100 g/day, s.c.) in adult male DA rats: (i) increased the thymocyte proliferation and apoptosis, (ii) caused disturbances in kinetics of T cell differentiation leading to distinguishable changes in relative proportion of thymocytes at distinct maturational steps and to an expansion of the most mature single positive (CD4+, CD8+) thymocyte pool, (iii) affected the relative proportion of neither CD4+ nor CD8+ peripheral blood lymphocytes (PBL), and (iv) augmented the relative number of CD8+CD25+ cells. Thus, the results suggest the role of beta-adrenoceptors in fine-tuning of T cell maturation, and, possibly, distribution and activation of distinct PBL subsets.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Neuroscience
T1  - Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes
EP  - 1673
IS  - 12
SP  - 1653
VL  - 113
DO  - 10.1080/00207450390245216
ER  - 

@article{
author = "Rauški, Aleksandra and Kosec, Duško and Vidić-Danković, Biljana and Plećaš-Solarović, Bosiljka and Leposavić, Gordana",
year = "2003",
abstract = "The study revealed that beta-adrenoceptor blockade with propranolol (0.40 mg/100 g/day, s.c.) in adult male DA rats: (i) increased the thymocyte proliferation and apoptosis, (ii) caused disturbances in kinetics of T cell differentiation leading to distinguishable changes in relative proportion of thymocytes at distinct maturational steps and to an expansion of the most mature single positive (CD4+, CD8+) thymocyte pool, (iii) affected the relative proportion of neither CD4+ nor CD8+ peripheral blood lymphocytes (PBL), and (iv) augmented the relative number of CD8+CD25+ cells. Thus, the results suggest the role of beta-adrenoceptors in fine-tuning of T cell maturation, and, possibly, distribution and activation of distinct PBL subsets.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Neuroscience",
title = "Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes",
pages = "1673-1653",
number = "12",
volume = "113",
doi = "10.1080/00207450390245216"
}

Rauški, A., Kosec, D., Vidić-Danković, B., Plećaš-Solarović, B.,& Leposavić, G.. (2003). Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes. in International Journal of Neuroscience
Taylor & Francis Ltd, Abingdon., 113(12), 1653-1673.
https://doi.org/10.1080/00207450390245216

Rauški A, Kosec D, Vidić-Danković B, Plećaš-Solarović B, Leposavić G. Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes. in International Journal of Neuroscience. 2003;113(12):1653-1673.
doi:10.1080/00207450390245216 .

Rauški, Aleksandra, Kosec, Duško, Vidić-Danković, Biljana, Plećaš-Solarović, Bosiljka, Leposavić, Gordana, "Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes" in International Journal of Neuroscience, 113, no. 12 (2003):1653-1673,
https://doi.org/10.1080/00207450390245216 . .

TY  - JOUR
AU  - Rauški, Aleksandra
AU  - Kosec, Duško
AU  - Vidić-Danković, Biljana
AU  - Radojević, Katarina
AU  - Plećaš-Solarović, Bosiljka
AU  - Leposavić, Gordana
PY  - 2003
UR  - http://intor.torlakinstitut.com/handle/123456789/161
AB  - The present study was undertaken in order to further clarify putative role of the adrenergic innervation in the regulation of the intrathymic T-cell maturation. For this purpose adult male DA rats were subjected to either 4-day- or 16-day-long propranolol treatment (0.40 mg propranolol/100 g/day, s.c.) and the expression of CD4/8/TCRalphabeta on thymocytes, as well as thymocyte proliferative and apoptotic index, was assessed in these animals by flow cytometric analysis. Propranolol treatment, in spite of duration, increased both the thymocyte proliferative and apoptotic index (vs. respective vehicle-treated controls). In 4-day-treated animals the thymus cellularity and thymus weight remained unaltered, while in 16-day-treated rats the values of both of these parameters were reduced (since increase in the thymocyte apoptotic index overcame that in the proliferative index). The treatments of both durations affected the thymocyte phenotypic profile in a similar pattern, but the changes were more pronounced in rats exposed to the treatment of longer duration. The relative proportion of the least mature CD4-8- double negative (DN) TCRalphabeta(-) cells was increased, those of thymocytes at distinct differentiational stages on the transitional route to the CD4+8+ double positive (DP) TCRalphabeta(low) stage decreased (all subsets of TCRalphabeta(-) in both groups of rats, and those. with low expression of TCRalphabeta in rats subjected to 16-day-long treatment) or unaltered (all subsets of TCRalphabeta(low) cells in 4-day-treated rats). Furthermore, the percentage of CD4+8+ DP TCRalphabeta(low) cells was significantly elevated, as well as those of the most mature CD4+8-TCRalphabeta(high) and CD4-8+TCRalphabeta(high) cells (the increase in the percentage of former was much more conspicuous than that of the latter), while the relative proportion of their direct detectable precursors (CD4+8+ DP TCRalphabeta(high)) Was reduced. Thus, the present study: i) further supports notion of pharmacological manipulation of adrenergic action as an efficient means in modulation of the T-cell development, and hence T-cell-dependent immune response, and ii) provides more specific insight into T-cell maturation sequence point/s particularly sensitive to beta-adrenoceptor ligand action.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Immunopharmacology and Immunotoxicology
T1  - Thymopoiesis following chronic blockade of beta-adrenoceptors
EP  - 528
IS  - 4
SP  - 513
VL  - 25
DO  - 10.1081/IPH-120026437
ER  - 

@article{
author = "Rauški, Aleksandra and Kosec, Duško and Vidić-Danković, Biljana and Radojević, Katarina and Plećaš-Solarović, Bosiljka and Leposavić, Gordana",
year = "2003",
abstract = "The present study was undertaken in order to further clarify putative role of the adrenergic innervation in the regulation of the intrathymic T-cell maturation. For this purpose adult male DA rats were subjected to either 4-day- or 16-day-long propranolol treatment (0.40 mg propranolol/100 g/day, s.c.) and the expression of CD4/8/TCRalphabeta on thymocytes, as well as thymocyte proliferative and apoptotic index, was assessed in these animals by flow cytometric analysis. Propranolol treatment, in spite of duration, increased both the thymocyte proliferative and apoptotic index (vs. respective vehicle-treated controls). In 4-day-treated animals the thymus cellularity and thymus weight remained unaltered, while in 16-day-treated rats the values of both of these parameters were reduced (since increase in the thymocyte apoptotic index overcame that in the proliferative index). The treatments of both durations affected the thymocyte phenotypic profile in a similar pattern, but the changes were more pronounced in rats exposed to the treatment of longer duration. The relative proportion of the least mature CD4-8- double negative (DN) TCRalphabeta(-) cells was increased, those of thymocytes at distinct differentiational stages on the transitional route to the CD4+8+ double positive (DP) TCRalphabeta(low) stage decreased (all subsets of TCRalphabeta(-) in both groups of rats, and those. with low expression of TCRalphabeta in rats subjected to 16-day-long treatment) or unaltered (all subsets of TCRalphabeta(low) cells in 4-day-treated rats). Furthermore, the percentage of CD4+8+ DP TCRalphabeta(low) cells was significantly elevated, as well as those of the most mature CD4+8-TCRalphabeta(high) and CD4-8+TCRalphabeta(high) cells (the increase in the percentage of former was much more conspicuous than that of the latter), while the relative proportion of their direct detectable precursors (CD4+8+ DP TCRalphabeta(high)) Was reduced. Thus, the present study: i) further supports notion of pharmacological manipulation of adrenergic action as an efficient means in modulation of the T-cell development, and hence T-cell-dependent immune response, and ii) provides more specific insight into T-cell maturation sequence point/s particularly sensitive to beta-adrenoceptor ligand action.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Immunopharmacology and Immunotoxicology",
title = "Thymopoiesis following chronic blockade of beta-adrenoceptors",
pages = "528-513",
number = "4",
volume = "25",
doi = "10.1081/IPH-120026437"
}

Rauški, A., Kosec, D., Vidić-Danković, B., Radojević, K., Plećaš-Solarović, B.,& Leposavić, G.. (2003). Thymopoiesis following chronic blockade of beta-adrenoceptors. in Immunopharmacology and Immunotoxicology
Taylor & Francis Ltd, Abingdon., 25(4), 513-528.
https://doi.org/10.1081/IPH-120026437

Rauški A, Kosec D, Vidić-Danković B, Radojević K, Plećaš-Solarović B, Leposavić G. Thymopoiesis following chronic blockade of beta-adrenoceptors. in Immunopharmacology and Immunotoxicology. 2003;25(4):513-528.
doi:10.1081/IPH-120026437 .

Rauški, Aleksandra, Kosec, Duško, Vidić-Danković, Biljana, Radojević, Katarina, Plećaš-Solarović, Bosiljka, Leposavić, Gordana, "Thymopoiesis following chronic blockade of beta-adrenoceptors" in Immunopharmacology and Immunotoxicology, 25, no. 4 (2003):513-528,
https://doi.org/10.1081/IPH-120026437 . .

TY  - JOUR
AU  - Ilić, V.
AU  - Lazarević, M.
AU  - Rauški, Aleksandra
AU  - Popović, N.
AU  - Kosec, Duško
PY  - 2001
UR  - http://intor.torlakinstitut.com/handle/123456789/133
AB  - We investigated the influence of an antiparasitic drug, levamisole (2,3,5,6 - tetrahydro - 6- phenyl-imidazo (2,1 - b) thiazole -hydrochloride) with potent immunomodulatory properties on the course and development of experimental autoimmune encepha-lomyelitis (EAE). EAE was induced in female Dark Agouti (DA) rats aged two months by immunization with guinea pig spinal cord in complete Freunds adjuvant. Following immunization animals were subcutaneously treated every other day with 2.2 mg/kg levamisole. The course, development and characteristics of this autoimmune process were monitored as indirect indicators of immune system activity. Our results indicate that in EAE levamisole exerts immunosuppressive effects when administered every other day from the moment of immunization until the end of the disease. This application regime and dose postponed the onset of the first clinical signs, shortened the duration of the disease, abrogated the severity of clinical symptoms and accelerated the recovery of sick animals. In the period of induction and during EAE, levamisole also decreased the severity of changes in the cerebral perivascular spaces. In the peripheral blood of levamisole treated animals with induced EAE, a significant increase of CD4-CD8+ T cells was demonstrated. Furthermore, all rats with induced EAE had decreased numbers of CD4+CD8- T cells in their blood. These changes were in correlation with clinical signs of EAE.
AB  - U ovom radu su prikazani rezultati ispitivanja anthelmintika levamizola (2,3,5,6 tetrahidro - 6 - fenil - imidazo (2,1 - b) tiazol hidrohlorida) sa snažnim imunomodulatornim svojstvima na tok i razvoj eksperimentalnog autoimunog encefalomijelitisa (EAE). EAE je indukovan imunizacijom ženki pacova soja DA (Dark Agouti) starih dva meseca pomoću homogenata kičmene moždine zamorčeta u kompletnom Freundovom adjuvansu. Posle imunizacije, životinje su tretirane subkutanim injekcijama levamizola (2.2 mg/kg) svaki drugi dan a praćeni su tok, razvoj i karakteristike ovog autoimunog oboljenja kao indirektni indikatori aktivnosti imunološkog sistema. Postignuti rezultati ukazuju dalevamizol ispoljava imunosupresivno delovanje u modelu EAE ako se aplikuje svaki drugi dan od momenta imunizacije do kraja bolesti. Primenjena doza i režim aplikacije odložili su momenat pojavljivanja prvih kliničkih simptoma, skratili trajanje bolesti, ublažili ispoljavanje simptoma i ubrzali oporavak bolesnih životinja. U periodu indukcije i tokom EAE-a levamizol je smanjio stepen promena u cerebralnim perivaskularnim prostorima. U ženskoj krvi ženki pacova sa indukovanim EAE i tretiranim levamizolom uočeno je značajno povećanje broja CD4-CD8+ T ćelija. Osim toga, u obe imunizovane grupe životinja zapaženo je smanjenje broja CD4+CD8- ćelija. Ove promene su bile u skladu sa kliničkom slikom bolesti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria - Beograd
T1  - Levamisole modulates experimental autoimmune encephalomyelitis (EAE) in DA rats
T1  - Modulacija eksperimentalnog autoimunog encefalomijelitisa (EAE) DA pacova levamizolom
EP  - 100
IS  - 2-3
SP  - 89
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_171
ER  - 

@article{
author = "Ilić, V. and Lazarević, M. and Rauški, Aleksandra and Popović, N. and Kosec, Duško",
year = "2001",
abstract = "We investigated the influence of an antiparasitic drug, levamisole (2,3,5,6 - tetrahydro - 6- phenyl-imidazo (2,1 - b) thiazole -hydrochloride) with potent immunomodulatory properties on the course and development of experimental autoimmune encepha-lomyelitis (EAE). EAE was induced in female Dark Agouti (DA) rats aged two months by immunization with guinea pig spinal cord in complete Freunds adjuvant. Following immunization animals were subcutaneously treated every other day with 2.2 mg/kg levamisole. The course, development and characteristics of this autoimmune process were monitored as indirect indicators of immune system activity. Our results indicate that in EAE levamisole exerts immunosuppressive effects when administered every other day from the moment of immunization until the end of the disease. This application regime and dose postponed the onset of the first clinical signs, shortened the duration of the disease, abrogated the severity of clinical symptoms and accelerated the recovery of sick animals. In the period of induction and during EAE, levamisole also decreased the severity of changes in the cerebral perivascular spaces. In the peripheral blood of levamisole treated animals with induced EAE, a significant increase of CD4-CD8+ T cells was demonstrated. Furthermore, all rats with induced EAE had decreased numbers of CD4+CD8- T cells in their blood. These changes were in correlation with clinical signs of EAE., U ovom radu su prikazani rezultati ispitivanja anthelmintika levamizola (2,3,5,6 tetrahidro - 6 - fenil - imidazo (2,1 - b) tiazol hidrohlorida) sa snažnim imunomodulatornim svojstvima na tok i razvoj eksperimentalnog autoimunog encefalomijelitisa (EAE). EAE je indukovan imunizacijom ženki pacova soja DA (Dark Agouti) starih dva meseca pomoću homogenata kičmene moždine zamorčeta u kompletnom Freundovom adjuvansu. Posle imunizacije, životinje su tretirane subkutanim injekcijama levamizola (2.2 mg/kg) svaki drugi dan a praćeni su tok, razvoj i karakteristike ovog autoimunog oboljenja kao indirektni indikatori aktivnosti imunološkog sistema. Postignuti rezultati ukazuju dalevamizol ispoljava imunosupresivno delovanje u modelu EAE ako se aplikuje svaki drugi dan od momenta imunizacije do kraja bolesti. Primenjena doza i režim aplikacije odložili su momenat pojavljivanja prvih kliničkih simptoma, skratili trajanje bolesti, ublažili ispoljavanje simptoma i ubrzali oporavak bolesnih životinja. U periodu indukcije i tokom EAE-a levamizol je smanjio stepen promena u cerebralnim perivaskularnim prostorima. U ženskoj krvi ženki pacova sa indukovanim EAE i tretiranim levamizolom uočeno je značajno povećanje broja CD4-CD8+ T ćelija. Osim toga, u obe imunizovane grupe životinja zapaženo je smanjenje broja CD4+CD8- ćelija. Ove promene su bile u skladu sa kliničkom slikom bolesti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria - Beograd",
title = "Levamisole modulates experimental autoimmune encephalomyelitis (EAE) in DA rats, Modulacija eksperimentalnog autoimunog encefalomijelitisa (EAE) DA pacova levamizolom",
pages = "100-89",
number = "2-3",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_171"
}

Ilić, V., Lazarević, M., Rauški, A., Popović, N.,& Kosec, D.. (2001). Levamisole modulates experimental autoimmune encephalomyelitis (EAE) in DA rats. in Acta veterinaria - Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 51(2-3), 89-100.
https://hdl.handle.net/21.15107/rcub_veterinar_171

Ilić V, Lazarević M, Rauški A, Popović N, Kosec D. Levamisole modulates experimental autoimmune encephalomyelitis (EAE) in DA rats. in Acta veterinaria - Beograd. 2001;51(2-3):89-100.
https://hdl.handle.net/21.15107/rcub_veterinar_171 .

Ilić, V., Lazarević, M., Rauški, Aleksandra, Popović, N., Kosec, Duško, "Levamisole modulates experimental autoimmune encephalomyelitis (EAE) in DA rats" in Acta veterinaria - Beograd, 51, no. 2-3 (2001):89-100,
https://hdl.handle.net/21.15107/rcub_veterinar_171 .

TY  - JOUR
AU  - Radojević, Katarina
AU  - Velikinac, S.
AU  - Rauški, Aleksandra
AU  - Vidić, Biljana
AU  - Jovanova-Nešić, Katica
PY  - 1999
UR  - http://intor.torlakinstitut.com/handle/123456789/106
AB  - In the present study we investigated the effect of selectivity destroyed dopaminergic neurons of the caudate-putamen (CP) on immune reactivity in the rat. Unilateral and bilateral lesioning of CP was performed by one direct stereotaxic injection of 6-hydroxydopamine solution. Sham-lesioned and intact rats served as controls. Two weeks after the operation, the animals were immunized with sheep red blood cells or bovine serum albumin for determination of plaque forming cell-response and Arthus and delayed hypersensitivity skin reactions, respectively. Unilateral and bilateral lesions of CP considerably suppressed PFC-response and Arthus and delayed the hypersensitivity reactions in comparison to control rats, while no differences were observed between unilaterally and bilaterally lesioned animals. (C) 1999 Elsevier Science Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Parkinsonism & Related Disorders
T1  - Effect of unilateral and bilateral lesions of caudate-putamen on immune response in the rat
EP  - 82
IS  - 1-2
SP  - 77
VL  - 5
DO  - 10.1016/S1353-8020(99)00014-0
ER  - 

@article{
author = "Radojević, Katarina and Velikinac, S. and Rauški, Aleksandra and Vidić, Biljana and Jovanova-Nešić, Katica",
year = "1999",
abstract = "In the present study we investigated the effect of selectivity destroyed dopaminergic neurons of the caudate-putamen (CP) on immune reactivity in the rat. Unilateral and bilateral lesioning of CP was performed by one direct stereotaxic injection of 6-hydroxydopamine solution. Sham-lesioned and intact rats served as controls. Two weeks after the operation, the animals were immunized with sheep red blood cells or bovine serum albumin for determination of plaque forming cell-response and Arthus and delayed hypersensitivity skin reactions, respectively. Unilateral and bilateral lesions of CP considerably suppressed PFC-response and Arthus and delayed the hypersensitivity reactions in comparison to control rats, while no differences were observed between unilaterally and bilaterally lesioned animals. (C) 1999 Elsevier Science Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Parkinsonism & Related Disorders",
title = "Effect of unilateral and bilateral lesions of caudate-putamen on immune response in the rat",
pages = "82-77",
number = "1-2",
volume = "5",
doi = "10.1016/S1353-8020(99)00014-0"
}

Radojević, K., Velikinac, S., Rauški, A., Vidić, B.,& Jovanova-Nešić, K.. (1999). Effect of unilateral and bilateral lesions of caudate-putamen on immune response in the rat. in Parkinsonism & Related Disorders
Elsevier Sci Ltd, Oxford., 5(1-2), 77-82.
https://doi.org/10.1016/S1353-8020(99)00014-0

Radojević K, Velikinac S, Rauški A, Vidić B, Jovanova-Nešić K. Effect of unilateral and bilateral lesions of caudate-putamen on immune response in the rat. in Parkinsonism & Related Disorders. 1999;5(1-2):77-82.
doi:10.1016/S1353-8020(99)00014-0 .

Radojević, Katarina, Velikinac, S., Rauški, Aleksandra, Vidić, Biljana, Jovanova-Nešić, Katica, "Effect of unilateral and bilateral lesions of caudate-putamen on immune response in the rat" in Parkinsonism & Related Disorders, 5, no. 1-2 (1999):77-82,
https://doi.org/10.1016/S1353-8020(99)00014-0 . .

TY  - CONF
AU  - Cupić, V.
AU  - Rauški, Aleksandra
AU  - Varagić, V.M.
PY  - 1998
UR  - http://intor.torlakinstitut.com/handle/123456789/88
PB  - Springer, New York
C3  - Naunyn-Schmiedebergs Archives of Pharmacology
T1  - The effect of xylazine on the mitogen-stimulated proliferative ability of thymus and spleen cells in culture in vitro
EP  - R751
IS  - 1
SP  - R751
VL  - 358
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_93
ER  - 

@conference{
author = "Cupić, V. and Rauški, Aleksandra and Varagić, V.M.",
year = "1998",
publisher = "Springer, New York",
journal = "Naunyn-Schmiedebergs Archives of Pharmacology",
title = "The effect of xylazine on the mitogen-stimulated proliferative ability of thymus and spleen cells in culture in vitro",
pages = "R751-R751",
number = "1",
volume = "358",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_93"
}

Cupić, V., Rauški, A.,& Varagić, V.M.. (1998). The effect of xylazine on the mitogen-stimulated proliferative ability of thymus and spleen cells in culture in vitro. in Naunyn-Schmiedebergs Archives of Pharmacology
Springer, New York., 358(1), R751-R751.
https://hdl.handle.net/21.15107/rcub_veterinar_93

Cupić V, Rauški A, Varagić V. The effect of xylazine on the mitogen-stimulated proliferative ability of thymus and spleen cells in culture in vitro. in Naunyn-Schmiedebergs Archives of Pharmacology. 1998;358(1):R751-R751.
https://hdl.handle.net/21.15107/rcub_veterinar_93 .

Cupić, V., Rauški, Aleksandra, Varagić, V.M., "The effect of xylazine on the mitogen-stimulated proliferative ability of thymus and spleen cells in culture in vitro" in Naunyn-Schmiedebergs Archives of Pharmacology, 358, no. 1 (1998):R751-R751,
https://hdl.handle.net/21.15107/rcub_veterinar_93 .