TY  - JOUR
AU  - Miljković, Marija
AU  - Thomas, Muriel
AU  - Serror, Pascale
AU  - Rigottier-Gois, Lionel
AU  - Kojić, Milan
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1259
UR  - http://intor.torlakinstitut.com/handle/123456789/701
AB  - Surface properties like hydrophobicity, aggregation ability, adhesion to mucosal surfaces and epithelial cells and transit time are key features for the characterization of probiotic strains. In this study, we used two Lactobacillus paracasei subsp. paracasei strains (BGNJ1-64 and BGSJ2-8) strains which were previously described with very strong aggregation capacity. The aggregation promoting factor (AggLb) expressed in these strains showed high level of binding to collagen and fibronectin, components of extracellular matrix. The working hypothesis was that strains able to aggregate have an advantage to resist in intestinal tract. So, we assessed whether these strains and their derivatives (without aggLb gene) are able to bind or not to intestinal components and we compared the transit time of each strains in mice. In that purpose parental strains (BGNJ1-64 and BGSJ2-8) and their aggregation negative derivatives (BGNJ1-641 and BGSJ2-83) were marked with double antibiotic resistance in order to be tracked in in vivo experiments in mice. Comparative analysis of binding ability of WT and aggregation negative strains to different human intestinal cell lines and mucin revealed no significant difference among them, excluding involvement of AggLb in interaction with surface of intestinal cells and mucin. In vivo experiments showed that surviving and transit time of marked strains in mice did not drastically depend on the presence of the AggLb aggregation factor.
PB  - Springer, Dordrecht
T2  - World Journal of Microbiology & Biotechnology
T1  - Binding activity to intestinal cells and transient colonization in mice of two Lactobacillus paracasei subsp. paracasei strains with high aggregation potential
IS  - 6
VL  - 35
DO  - 10.1007/s11274-019-2663-4
ER  - 

@article{
author = "Miljković, Marija and Thomas, Muriel and Serror, Pascale and Rigottier-Gois, Lionel and Kojić, Milan",
year = "2019",
abstract = "Surface properties like hydrophobicity, aggregation ability, adhesion to mucosal surfaces and epithelial cells and transit time are key features for the characterization of probiotic strains. In this study, we used two Lactobacillus paracasei subsp. paracasei strains (BGNJ1-64 and BGSJ2-8) strains which were previously described with very strong aggregation capacity. The aggregation promoting factor (AggLb) expressed in these strains showed high level of binding to collagen and fibronectin, components of extracellular matrix. The working hypothesis was that strains able to aggregate have an advantage to resist in intestinal tract. So, we assessed whether these strains and their derivatives (without aggLb gene) are able to bind or not to intestinal components and we compared the transit time of each strains in mice. In that purpose parental strains (BGNJ1-64 and BGSJ2-8) and their aggregation negative derivatives (BGNJ1-641 and BGSJ2-83) were marked with double antibiotic resistance in order to be tracked in in vivo experiments in mice. Comparative analysis of binding ability of WT and aggregation negative strains to different human intestinal cell lines and mucin revealed no significant difference among them, excluding involvement of AggLb in interaction with surface of intestinal cells and mucin. In vivo experiments showed that surviving and transit time of marked strains in mice did not drastically depend on the presence of the AggLb aggregation factor.",
publisher = "Springer, Dordrecht",
journal = "World Journal of Microbiology & Biotechnology",
title = "Binding activity to intestinal cells and transient colonization in mice of two Lactobacillus paracasei subsp. paracasei strains with high aggregation potential",
number = "6",
volume = "35",
doi = "10.1007/s11274-019-2663-4"
}

Miljković, M., Thomas, M., Serror, P., Rigottier-Gois, L.,& Kojić, M.. (2019). Binding activity to intestinal cells and transient colonization in mice of two Lactobacillus paracasei subsp. paracasei strains with high aggregation potential. in World Journal of Microbiology & Biotechnology
Springer, Dordrecht., 35(6).
https://doi.org/10.1007/s11274-019-2663-4

Miljković M, Thomas M, Serror P, Rigottier-Gois L, Kojić M. Binding activity to intestinal cells and transient colonization in mice of two Lactobacillus paracasei subsp. paracasei strains with high aggregation potential. in World Journal of Microbiology & Biotechnology. 2019;35(6).
doi:10.1007/s11274-019-2663-4 .

Miljković, Marija, Thomas, Muriel, Serror, Pascale, Rigottier-Gois, Lionel, Kojić, Milan, "Binding activity to intestinal cells and transient colonization in mice of two Lactobacillus paracasei subsp. paracasei strains with high aggregation potential" in World Journal of Microbiology & Biotechnology, 35, no. 6 (2019),
https://doi.org/10.1007/s11274-019-2663-4 . .